TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3713493.2.1
(696 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI960320.1|BI960320 HVSMEn0024D13f Hordeum vulgare rachi... 133 1e-030
gb|BM376087.2|BM376087 EBma01_SQ002_E24_R maternal, 4 DPA, ... 132 2e-030
gb|BJ548121.1|BJ548121 BJ548121 K. Sato unpublished cDNA li... 131 4e-030
gb|BJ548884.1|BJ548884 BJ548884 K. Sato unpublished cDNA li... 131 4e-030
gb|BI956408.1|BI956408 HVSMEn0003C22f Hordeum vulgare rachi... 130 9e-030
gb|AL512051.1|AL512051 AL512051 Hordeum vulgare Barke devel... 118 4e-026
gb|BI956401.1|BI956401 HVSMEn0003C12f Hordeum vulgare rachi... 100 9e-021
>gb|BI960320.1|BI960320 HVSMEn0024D13f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024D13f, mRNA sequence
Length = 653
Score = 133 bits (285), Expect = 1e-030
Identities = 51/75 (68%), Positives = 61/75 (81%)
Frame = -2 / -1
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD VTP+KL FD + +SL YEIT+AV+GNPVIVD YSFGS+TWSDGAH
Sbjct: 341 YEAKVESPAGVDAKVTPAKLVFDEEHRSLAYEITLAVAGNPVIVDGKYSFGSVTWSDGAH 162
Query: 512 DVTSPIAVTWPSNGG 468
+VTSPIAVTWP + G
Sbjct: 161 NVTSPIAVTWPESAG 117
Score = 119 bits (255), Expect = 1e-026
Identities = 55/81 (67%), Positives = 59/81 (72%)
Frame = +1 / +1
Query: 436 ATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLC 615
+ A+ YM AAP L G VTAMGLVT CAPSL V DPNE L STITGLP TA VISY RL
Sbjct: 85 SVATSLYMDAAPALSGHVTAMGLVTLCAPSLHVTDPNEYLPSTITGLPATARVISYARLR 264
Query: 616 CFPSNASLLGVTVTSTPYGLA 678
C SN SL GVT+ STP GL+
Sbjct: 265 CSSSNTSLAGVTLASTPAGLS 327
>gb|BM376087.2|BM376087 EBma01_SQ002_E24_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ002_E24 5', mRNA sequence
Length = 436
Score = 132 bits (283), Expect = 2e-030
Identities = 51/91 (56%), Positives = 62/91 (68%)
Frame = -2 / +2
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD VTP+KL FD + +SL YEIT+AV+GNPVIVD YSFGS+TWSDG H
Sbjct: 92 YEAKVESPAGVDAKVTPAKLVFDEEHRSLAYEITLAVAGNPVIVDGKYSFGSVTWSDGVH 271
Query: 512 DVTSPIAVTWPSNGGXXXXXXXXXXXXVYRC 420
+VTSPIAVTWP + G + C
Sbjct: 272 NVTSPIAVTWPESAGAASMYRDVATLVSHDC 364
Score = 117 bits (251), Expect = 5e-026
Identities = 54/81 (66%), Positives = 58/81 (71%)
Frame = +1 / -2
Query: 436 ATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLC 615
+ A+ YM AAP L G VTAMGLVT C PSL V DPNE L STITGLP TA VISY RL
Sbjct: 348 SVATSLYMDAAPALSGHVTAMGLVTLCTPSLHVTDPNEYLPSTITGLPATARVISYARLR 169
Query: 616 CFPSNASLLGVTVTSTPYGLA 678
C SN SL GVT+ STP GL+
Sbjct: 168 CSSSNTSLAGVTLASTPAGLS 106
>gb|BJ548121.1|BJ548121 BJ548121 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal19e21 3', mRNA sequence
Length = 584
Score = 131 bits (281), Expect = 4e-030
Identities = 50/75 (66%), Positives = 60/75 (80%)
Frame = -2 / -1
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD VTP+KL FD + +SL YEIT+AV+GNPVIVD YSFGS+TWSDG H
Sbjct: 491 YEAKVESPAGVDAKVTPAKLVFDEEHRSLAYEITLAVAGNPVIVDGKYSFGSVTWSDGVH 312
Query: 512 DVTSPIAVTWPSNGG 468
+VTSPIAVTWP + G
Sbjct: 311 NVTSPIAVTWPESAG 267
Score = 117 bits (251), Expect = 5e-026
Identities = 54/81 (66%), Positives = 58/81 (71%)
Frame = +1 / +1
Query: 436 ATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLC 615
+ A+ YM AAP L G VTAMGLVT C PSL V DPNE L STITGLP TA VISY RL
Sbjct: 235 SVATSLYMDAAPALSGHVTAMGLVTLCTPSLHVTDPNEYLPSTITGLPATARVISYARLR 414
Query: 616 CFPSNASLLGVTVTSTPYGLA 678
C SN SL GVT+ STP GL+
Sbjct: 415 CSSSNTSLAGVTLASTPAGLS 477
>gb|BJ548884.1|BJ548884 BJ548884 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal37l10 3', mRNA sequence
Length = 523
Score = 131 bits (281), Expect = 4e-030
Identities = 50/75 (66%), Positives = 60/75 (80%)
Frame = -2 / -3
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD VTP+KL FD + +SL YEIT+AV+GNPVIVD YSFGS+TWSDG H
Sbjct: 488 YEAKVESPAGVDAKVTPAKLVFDEEHRSLAYEITLAVAGNPVIVDGKYSFGSVTWSDGVH 309
Query: 512 DVTSPIAVTWPSNGG 468
+VTSPIAVTWP + G
Sbjct: 308 NVTSPIAVTWPESAG 264
Score = 117 bits (251), Expect = 5e-026
Identities = 54/81 (66%), Positives = 58/81 (71%)
Frame = +1 / +1
Query: 436 ATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLC 615
+ A+ YM AAP L G VTAMGLVT C PSL V DPNE L STITGLP TA VISY RL
Sbjct: 232 SVATSLYMDAAPALSGHVTAMGLVTLCTPSLHVTDPNEYLPSTITGLPATARVISYARLR 411
Query: 616 CFPSNASLLGVTVTSTPYGLA 678
C SN SL GVT+ STP GL+
Sbjct: 412 CSSSNTSLAGVTLASTPAGLS 474
>gb|BI956408.1|BI956408 HVSMEn0003C22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0003C22f, mRNA sequence
Length = 637
Score = 130 bits (278), Expect = 9e-030
Identities = 50/75 (66%), Positives = 60/75 (80%)
Frame = -2 / +2
Query: 692 YQPTIASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAH 513
Y+ + SP GVD VTP+KL FD + +SL YEIT+AV+GNPVIVD YSFGS+TWSDGAH
Sbjct: 242 YEAKVESPAGVDAKVTPAKLVFDEEHRSLAYEITLAVAGNPVIVDGKYSFGSVTWSDGAH 421
Query: 512 DVTSPIAVTWPSNGG 468
+VTSPIA TWP + G
Sbjct: 422 NVTSPIAGTWPESAG 466
Score = 118 bits (253), Expect = 3e-026
Identities = 54/81 (66%), Positives = 59/81 (72%)
Frame = +1 / -2
Query: 436 ATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLC 615
+ A+ +YM AAP L G V AMGLVT CAPSL V DPNE L STITGLP TA VISY RL
Sbjct: 498 SVATSFYMDAAPALSGHVPAMGLVTLCAPSLHVTDPNEYLPSTITGLPATARVISYARLR 319
Query: 616 CFPSNASLLGVTVTSTPYGLA 678
C SN SL GVT+ STP GL+
Sbjct: 318 CSSSNTSLAGVTLASTPAGLS 256
>gb|AL512051.1|AL512051 AL512051 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY10D13u 3',
mRNA sequence
Length = 335
Score = 118 bits (252), Expect = 4e-026
Identities = 45/61 (73%), Positives = 53/61 (86%)
Frame = -2 / -2
Query: 650 VTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAHDVTSPIAVTWPSNG 471
VTP+KL FD + +SL YEIT+AV+GNPVIVD YSFGS+TWSDGAH+VTSPIAVTWP +
Sbjct: 334 VTPAKLVFDEEHRSLAYEITLAVAGNPVIVDGKYSFGSVTWSDGAHNVTSPIAVTWPESA 155
Query: 470 G 468
G
Sbjct: 154 G 152
Score = 107 bits (229), Expect = 5e-023
Identities = 50/72 (69%), Positives = 52/72 (72%)
Frame = +1 / +3
Query: 436 ATASVYYMAAAPPLDGQVTAMGLVTSCAPSLQVMDPNE*LESTITGLPDTAMVISYPRLC 615
+ A+ YM AAP L G VTAMGLVT CAPSL V DPNE L STITGLP TA VISY RL
Sbjct: 120 SVATSLYMDAAPALSGHVTAMGLVTLCAPSLHVTDPNEYLPSTITGLPATARVISYARLR 299
Query: 616 CFPSNASLLGVT 651
C SN SL GVT
Sbjct: 300 CSSSNTSLAGVT 335
>gb|BI956401.1|BI956401 HVSMEn0003C12f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0003C12f, mRNA sequence
Length = 878
Score = 100 bits (213), Expect = 9e-021
Identities = 40/66 (60%), Positives = 49/66 (74%)
Frame = -2 / +2
Query: 680 IASPYGVDVTVTPSKLAFDGKQQSLGYEITIAVSGNPVIVDSSYSFGSITWSDGAHDVTS 501
+ SP GVD VTP+KL F + +SL Y+IT+ V+GNPVIVD S G+ TWSDGAH+VTS
Sbjct: 254 VESPAGVDTKVTPAKLVFXEEHRSLAYKITLGVAGNPVIVDGKCSVGARTWSDGAHNVTS 433
Query: 500 PIAVTW 483
PI TW
Sbjct: 434 PIGGTW 451
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 403,687,789
Number of Sequences: 312970
Number of extensions: 7571391
Number of successful extensions: 93889
Number of sequences better than 1.0e-020: 11
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7635007620
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)