TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3696657.2.1
(1531 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow st... 144 8e-065
gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow st... 124 3e-053
gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow st... 191 1e-047
gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow st... 119 1e-043
gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow st... 124 1e-042
gb|BQ464404.1|BQ464404 HF02C24r HF Hordeum vulgare subsp. v... 139 4e-032
gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow st... 124 7e-032
gb|BQ470839.1|BQ470839 HX04D02r HX Hordeum vulgare subsp. v... 88 1e-027
gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. v... 111 4e-027
gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow st... 121 1e-026
gb|CA030305.1|CA030305 HX06K19r HX Hordeum vulgare subsp. v... 74 9e-025
gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachi... 105 6e-022
>gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G09 5', mRNA sequence
Length = 588
Score = 144 bits (309), Expect(2) = 8e-065
Identities = 54/90 (60%), Positives = 72/90 (80%)
Frame = -2 / +3
Query: 753 ITCGPGQGISVGCLGRYKDEKDVSDITVRNCVLKNTTNGVRIKSYVDAESVLTASHLTFE 574
+TCGPG GIS+G LGRYKDEKDV+DI V+NCV K +TNG+RIKSY D++S L AS +++E
Sbjct: 6 VTCGPGHGISIGSLGRYKDEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYE 185
Query: 573 NIRMEEVANPIVIDQYFCPQKVCPGKRSNS 484
NI+M++ PI+IDQ +CP K+C K +S
Sbjct: 186 NIKMDDSGYPIIIDQKYCPNKLCTAKGDSS 275
Score = 124 bits (265), Expect(2) = 8e-065
Identities = 50/75 (66%), Positives = 63/75 (84%)
Frame = -2 / +3
Query: 495 RSNSSHVSVKDVTFRNITGTSSTPEAISLLCSETQPCSGVSLIDVNVEYAGKNNKTMAVC 316
+ +SS V+VKDVTF+NITGTSSTPEA+SLLCS+ +PC GV++ DV +EY+GKNNKTMAVC
Sbjct: 261 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 440
Query: 315 SNAKGTAKGSIEALA 271
+N K TA G +A A
Sbjct: 441 TNVKVTATGVDKANA 485
Score = 100 bits (212), Expect(2) = 3e-026
Identities = 46/88 (52%), Positives = 53/88 (60%)
Frame = -1 / +2
Query: 748 LRPRAGHQRRLPRPLQGREGRERHHGAELRAQEHHQRRAHQVVRGRRVRADGLPSHLREH 569
LRPR HQ PR +QGREGR+RH ELR Q HQR HQVVRG +V + LREH
Sbjct: 11 LRPRPRHQYWQPREVQGREGRDRHPRQELRVQGIHQRPPHQVVRGLQVPPRRVQDQLREH 190
Query: 568 QDGGGGQPHRHRPVLLPAEGMPWQAEQL 485
QDG G PH HR +LP + + Q L
Sbjct: 191 QDGRLGLPHHHRSEVLPQQTLHRQGRLL 274
Score = 95.9 bits (203), Expect(2) = 2e-041
Identities = 47/72 (65%), Positives = 52/72 (72%)
Frame = +2 / -3
Query: 272 ASASMLPLAVPLALLQTAMVLLFLPAYSTLTSMRETPLHGCVSEQSRLMASGVDDVPVML 451
A A P+AV L L+QTAMVLLFLP YS LTS TP G +SEQSRL ASGV++VPVM
Sbjct: 484 ALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPSQGFLSEQSRLTASGVEEVPVMF 305
Query: 452 RNVTSLTETCEE 487
VTSLT T EE
Sbjct: 304 LKVTSLTVTLEE 269
Score = 94.5 bits (200), Expect(2) = 2e-041
Identities = 48/78 (61%), Positives = 54/78 (69%)
Frame = +2 / -3
Query: 518 GQKYWSMTMGLATSSILMFSKVRWEAVSTDSASTYDLMRTPLVVFLSTQFRTVMSLTSFS 697
GQ + SM MG SSILMFS + +A D S+YDLMR PLV L+TQF T MS+TSFS
Sbjct: 241 GQYF*SMMMG*PESSILMFS*LILDATRGDLESSYDLMRRPLVDPLNTQFLTWMSVTSFS 62
Query: 698 SL*RPRQPTLMPCPGPQV 751
SL PR P LMP PGPQV
Sbjct: 61 SLYLPRLPILMPWPGPQV 8
Score = 72.1 bits (151), Expect(2) = 8e-024
Identities = 33/61 (54%), Positives = 41/61 (67%)
Frame = +1 / -2
Query: 484 GVAPLARAYLLRAEVLVDDDGVGHLLHPDVLEGEMGGRQHGLGVHVRLDAHAVGGVLEHA 663
G L A + A +L+DDDGV ++H DVL ++G + GLGV VRLDA AVGG LEHA
Sbjct: 275 GGVALGGAEFVGAVLLIDDDGVARVVHLDVLVADLGRDEGGLGVLVRLDAEAVGGSLEHA 96
Query: 664 V 666
V
Sbjct: 95 V 93
Score = 59.3 bits (123), Expect(2) = 8e-024
Identities = 31/66 (46%), Positives = 35/66 (53%)
Frame = +1 / -2
Query: 295 GGALGVAADGHGFVVLAGVLHVDIDEGDAAAWLRLRAEQXXXXXXXXXXXXVAERDVLDG 474
GG V D HG VVLAGVL +D+ DA A L + AEQ V E +VLDG
Sbjct: 461 GGDFDVGTDSHGLVVLAGVLDLDVGHCDAFARLFVGAEQADGLRGGGGAGDVFEGNVLDG 282
Query: 475 DMRGVA 492
D GVA
Sbjct: 281 DPGGVA 264
Score = 42.3 bits (86), Expect(2) = 3e-026
Identities = 15/25 (60%), Positives = 20/25 (80%)
Frame = -3 / +1
Query: 353 STPARTTKPWPSAATPRAPPREASR 279
STPARTT+PW S T ++PPR ++R
Sbjct: 403 STPARTTRPWLSVPTSKSPPRGSTR 477
>gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_F02 5', mRNA sequence
Length = 693
Score = 124 bits (265), Expect(2) = 3e-053
Identities = 50/75 (66%), Positives = 63/75 (84%)
Frame = -2 / +2
Query: 495 RSNSSHVSVKDVTFRNITGTSSTPEAISLLCSETQPCSGVSLIDVNVEYAGKNNKTMAVC 316
+ +SS V+VKDVTF+NITGTSSTPEA+SLLCS+ +PC GV++ DV +EY+GKNNKTMAVC
Sbjct: 203 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 382
Query: 315 SNAKGTAKGSIEALA 271
+N K TA G +A A
Sbjct: 383 TNVKVTATGVDKANA 427
Score = 105 bits (224), Expect(2) = 3e-053
Identities = 40/72 (55%), Positives = 56/72 (77%)
Frame = -2 / +2
Query: 699 DEKDVSDITVRNCVLKNTTNGVRIKSYVDAESVLTASHLTFENIRMEEVANPIVIDQYFC 520
DEKDV+DI V+NCV K +TNG+RIKSY D++S L AS +++ENI+M++ PI+IDQ +C
Sbjct: 2 DEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYC 181
Query: 519 PQKVCPGKRSNS 484
P K+C K +S
Sbjct: 182 PNKLCTAKGDSS 217
Score = 95.9 bits (203), Expect(2) = 1e-034
Identities = 47/72 (65%), Positives = 52/72 (72%)
Frame = +2 / -1
Query: 272 ASASMLPLAVPLALLQTAMVLLFLPAYSTLTSMRETPLHGCVSEQSRLMASGVDDVPVML 451
A A P+AV L L+QTAMVLLFLP YS LTS TP G +SEQSRL ASGV++VPVM
Sbjct: 426 ALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPSQGFLSEQSRLTASGVEEVPVMF 247
Query: 452 RNVTSLTETCEE 487
VTSLT T EE
Sbjct: 246 LKVTSLTVTLEE 211
Score = 72.1 bits (151), Expect(2) = 1e-034
Identities = 33/61 (54%), Positives = 41/61 (67%)
Frame = +1 / -3
Query: 484 GVAPLARAYLLRAEVLVDDDGVGHLLHPDVLEGEMGGRQHGLGVHVRLDAHAVGGVLEHA 663
G L A + A +L+DDDGV ++H DVL ++G + GLGV VRLDA AVGG LEHA
Sbjct: 217 GGVALGGAEFVGAVLLIDDDGVARVVHLDVLVADLGRDEGGLGVLVRLDAEAVGGSLEHA 38
Query: 664 V 666
V
Sbjct: 37 V 35
Score = 68.4 bits (143), Expect(2) = 1e-022
Identities = 36/61 (59%), Positives = 42/61 (68%)
Frame = +2 / -1
Query: 518 GQKYWSMTMGLATSSILMFSKVRWEAVSTDSASTYDLMRTPLVVFLSTQFRTVMSLTSFS 697
GQ + SM MG SSILMFS + +A D S+YDLMR PLV L+TQF T MS+TSFS
Sbjct: 183 GQYF*SMMMG*PESSILMFS*LILDATRGDLESSYDLMRRPLVDPLNTQFLTWMSVTSFS 4
Query: 698 S 700
S
Sbjct: 3 S 1
Score = 59.3 bits (123), Expect(2) = 1e-022
Identities = 31/66 (46%), Positives = 35/66 (53%)
Frame = +1 / -3
Query: 295 GGALGVAADGHGFVVLAGVLHVDIDEGDAAAWLRLRAEQXXXXXXXXXXXXVAERDVLDG 474
GG V D HG VVLAGVL +D+ DA A L + AEQ V E +VLDG
Sbjct: 403 GGDFDVGTDSHGLVVLAGVLDLDVGHCDAFARLFVGAEQADGLRGGGGAGDVFEGNVLDG 224
Query: 475 DMRGVA 492
D GVA
Sbjct: 223 DPGGVA 206
>gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L02 5', mRNA sequence
Length = 377
Score = 191 bits (411), Expect = 1e-047
Identities = 70/123 (56%), Positives = 97/123 (78%)
Frame = -2 / +2
Query: 864 GVHVGGSSKISILNSTIGTGDDCVSIGPGCNGVLVDSITCGPGQGISVGCLGRYKDEKDV 685
G+H+G SS ++I+++TI GDDC+SIGPG V + +TCGP GIS+G LGRYKDEKDV
Sbjct: 2 GIHMGDSSNVNIIDTTIAVGDDCISIGPGSKQVNISGVTCGPSHGISIGSLGRYKDEKDV 181
Query: 684 SDITVRNCVLKNTTNGVRIKSYVDAESVLTASHLTFENIRMEEVANPIVIDQYFCPQKVC 505
+DI V+NCV K +TNG+RIKSY D++S L AS +++ENI+M++ + PI+IDQ +CP K+C
Sbjct: 182 TDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSSYPIIIDQKYCPHKLC 361
Query: 504 PGK 496
K
Sbjct: 362 TAK 370
Score = 129 bits (276), Expect = 4e-029
Identities = 66/116 (56%), Positives = 74/116 (63%)
Frame = +2 / -3
Query: 518 GQKYWSMTMGLATSSILMFSKVRWEAVSTDSASTYDLMRTPLVVFLSTQFRTVMSLTSFS 697
GQ + SM MG SSILMFS + +A D S+YDLMR PLV L+TQF T MS+TSFS
Sbjct: 348 GQYF*SMMMG*LESSILMFS*LILDATRGDLESSYDLMRRPLVDPLNTQFLTWMSVTSFS 169
Query: 698 SL*RPRQPTLMPCPGPQVMLSTKTPLHPGPIETQSSPVPMVELRMLILEEPPTWTP 865
SL PR P LMP GPQV T L PGP+E QSSP MV ML L+E P W P
Sbjct: 168 SLYLPRLPILMPWLGPQVTPLMLTCLEPGPMEMQSSPTAMVVSMMLTLDESPMWMP 1
>gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E14 5', mRNA sequence
Length = 454
Score = 119 bits (254), Expect(2) = 1e-043
Identities = 49/75 (65%), Positives = 62/75 (82%)
Frame = -2 / +3
Query: 495 RSNSSHVSVKDVTFRNITGTSSTPEAISLLCSETQPCSGVSLIDVNVEYAGKNNKTMAVC 316
+ +SS V+VKDVTF+NITGTSSTPEA+SLLCS+ +P GV++ DV +EY+GKNNKTMAVC
Sbjct: 168 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPYEGVTMSDVKIEYSGKNNKTMAVC 347
Query: 315 SNAKGTAKGSIEALA 271
+N K TA G +A A
Sbjct: 348 TNVKVTATGVDKANA 392
Score = 94.1 bits (199), Expect(2) = 5e-032
Identities = 47/72 (65%), Positives = 52/72 (72%)
Frame = +2 / -1
Query: 272 ASASMLPLAVPLALLQTAMVLLFLPAYSTLTSMRETPLHGCVSEQSRLMASGVDDVPVML 451
A A P+AV L L+QTAMVLLFLP YS LTS TP G +SEQSRL ASGV++VPVM
Sbjct: 391 ALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPS*GFLSEQSRLTASGVEEVPVMF 212
Query: 452 RNVTSLTETCEE 487
VTSLT T EE
Sbjct: 211 LKVTSLTVTLEE 176
Score = 78.5 bits (165), Expect(2) = 1e-043
Identities = 29/59 (49%), Positives = 44/59 (74%)
Frame = -2 / +3
Query: 660 VLKNTTNGVRIKSYVDAESVLTASHLTFENIRMEEVANPIVIDQYFCPQKVCPGKRSNS 484
+ K +TNG+RIKSY D++S L AS +++ENI+M++ PI+IDQ +CP K+C K +S
Sbjct: 6 LFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDSS 182
Score = 64.8 bits (135), Expect(2) = 5e-032
Identities = 30/58 (51%), Positives = 38/58 (65%)
Frame = +1 / -3
Query: 484 GVAPLARAYLLRAEVLVDDDGVGHLLHPDVLEGEMGGRQHGLGVHVRLDAHAVGGVLE 657
G L A + A +L+DDDGV ++H DVL ++G + GLGV VRLDA AVGG LE
Sbjct: 182 GGVALGGAEFVGAVLLIDDDGVARVVHLDVLVADLGRDEGGLGVLVRLDAEAVGGSLE 9
>gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_L07 5', mRNA sequence
Length = 436
Score = 124 bits (265), Expect(2) = 1e-042
Identities = 50/75 (66%), Positives = 63/75 (84%)
Frame = -2 / +2
Query: 495 RSNSSHVSVKDVTFRNITGTSSTPEAISLLCSETQPCSGVSLIDVNVEYAGKNNKTMAVC 316
+ +SS V+VKDVTF+NITGTSSTPEA+SLLCS+ +PC GV++ DV +EY+GKNNKTMAVC
Sbjct: 143 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 322
Query: 315 SNAKGTAKGSIEALA 271
+N K TA G +A A
Sbjct: 323 TNVKVTATGVDKANA 367
Score = 95.9 bits (203), Expect(2) = 2e-029
Identities = 47/72 (65%), Positives = 52/72 (72%)
Frame = +2 / -2
Query: 272 ASASMLPLAVPLALLQTAMVLLFLPAYSTLTSMRETPLHGCVSEQSRLMASGVDDVPVML 451
A A P+AV L L+QTAMVLLFLP YS LTS TP G +SEQSRL ASGV++VPVM
Sbjct: 366 ALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPSQGFLSEQSRLTASGVEEVPVMF 187
Query: 452 RNVTSLTETCEE 487
VTSLT T EE
Sbjct: 186 LKVTSLTVTLEE 151
Score = 70.3 bits (147), Expect(2) = 1e-042
Identities = 26/52 (50%), Positives = 39/52 (75%)
Frame = -2 / +2
Query: 639 GVRIKSYVDAESVLTASHLTFENIRMEEVANPIVIDQYFCPQKVCPGKRSNS 484
G+RIKSY D++S L AS +++ENI+M++ PI+IDQ +CP K+C K +S
Sbjct: 2 GLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDSS 157
Score = 54.2 bits (112), Expect(2) = 2e-029
Identities = 25/52 (48%), Positives = 33/52 (63%)
Frame = +1 / -1
Query: 484 GVAPLARAYLLRAEVLVDDDGVGHLLHPDVLEGEMGGRQHGLGVHVRLDAHA 639
G L A + A +L+DDDGV ++H DVL ++G + GLGV VRLDA A
Sbjct: 157 GGVALGGAEFVGAVLLIDDDGVARVVHLDVLVADLGRDEGGLGVLVRLDAEA 2
>gb|BQ464404.1|BQ464404 HF02C24r HF Hordeum vulgare subsp. vulgare cDNA clone HF02C24
5-PRIME, mRNA sequence
Length = 579
Score = 139 bits (298), Expect = 4e-032
Identities = 57/159 (35%), Positives = 92/159 (57%)
Frame = -2 / +2
Query: 1095 TGSGTLDGQGTAVYSKSKTDNVKAMPNTLVLFHVINATVAGIKLLNSKFFHINIDNSESI 916
T S D + Y++ + P L + N VAGI ++NS H+ DN + +
Sbjct: 86 TYSDPNDDEDDDTYNQELERMPRVKPTALRFYGSSNVVVAGITIVNSSQCHLKFDNCQGV 265
Query: 915 TVKDVNVTAPADVENTDGVHVGGSSKISILNSTIGTGDDCVSIGPGCNGVLVDSITCGPG 736
V D+ +++P + NTDG+H+ S +SI ++ + GDDC+SI GC+ + + ++ CGPG
Sbjct: 266 LVHDLTISSPENSLNTDGIHLQNSKDVSIHHTNLACGDDCISIQTGCSNIYIHNVNCGPG 445
Query: 735 QGISVGCLGRYKDEKDVSDITVRNCVLKNTTNGVRIKSY 619
GIS+G LGR + VS++TVR+ + T GVRIK++
Sbjct: 446 HGISIGGLGRDNTKACVSNVTVRDVNMFRTMTGVRIKTW 562
>gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_C11 5', mRNA sequence
Length = 647
Score = 124 bits (265), Expect(2) = 7e-032
Identities = 50/75 (66%), Positives = 63/75 (84%)
Frame = -2 / +1
Query: 495 RSNSSHVSVKDVTFRNITGTSSTPEAISLLCSETQPCSGVSLIDVNVEYAGKNNKTMAVC 316
+ +SS V+VKDVTF+NITGTSSTPEA+SLLCS+ +PC GV++ DV +EY+GKNNKTMAVC
Sbjct: 64 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 243
Query: 315 SNAKGTAKGSIEALA 271
+N K TA G +A A
Sbjct: 244 TNVKVTATGVDKANA 288
Score = 34.1 bits (68), Expect(2) = 7e-032
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -2 / +1
Query: 546 PIVIDQYFCPQKVCPGKRSNS 484
PI+IDQ +CP K+C K +S
Sbjct: 16 PIIIDQKYCPNKLCTAKGDSS 78
>gb|BQ470839.1|BQ470839 HX04D02r HX Hordeum vulgare subsp. vulgare cDNA clone HX04D02
5-PRIME, mRNA sequence
Length = 650
Score = 87.7 bits (185), Expect(2) = 1e-027
Identities = 40/123 (32%), Positives = 61/123 (49%)
Frame = -2 / +2
Query: 981 VAGIKLLNSKFFHINIDNSESITVKDVNVTAPADVENTDGVHVGGSSKISILNSTIGTGD 802
V G+ L N + H+ + + V +P +TDG+ + S + I + I TG
Sbjct: 65 VQGVTLQNGQQHHLTFTRCSNARASFLRVASPESSPDTDGIRLVNSISVHINDIHIATGG 244
Query: 801 DCVSIGPGCNGVLVDSITCGPGQGISVGCLGRYKDEKDVSDITVRNCVLKNTTNGVRIKS 622
CVS+ C V + I+CGPG GIS+G +G + I V + NTTNG+ IK+
Sbjct: 245 ACVSMAGNCTDVRLRYISCGPGNGISIGSIGETPVADRLEKIEVDTMFIANTTNGLLIKT 424
Query: 621 YVD 613
+ D
Sbjct: 425 WQD 433
Score = 56.5 bits (117), Expect(2) = 1e-027
Identities = 23/66 (34%), Positives = 40/66 (60%)
Frame = -2 / +2
Query: 594 ASHLTFENIRMEEVANPIVIDQYFCPQKVCPGKRSNSSHVSVKDVTFRNITGTSSTPEAI 415
A + F N+ M+ V+NPI+IDQY + G + + V+V+ + + NI GTS++ A+
Sbjct: 446 ARKVKFANVVMKNVSNPIIIDQYRSEHPIPCGSTAATRTVAVEKIDYVNIAGTSASKRAV 625
Query: 414 SLLCSE 397
+ CS+
Sbjct: 626 TFSCSD 643
>gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. vulgare cDNA clone HM10P12
3-PRIME, mRNA sequence
Length = 608
Score = 111 bits (236), Expect(2) = 4e-027
Identities = 47/136 (34%), Positives = 71/136 (52%)
Frame = -2 / +3
Query: 1029 KAMPNTLVLFHVINATVAGIKLLNSKFFHINIDNSESITVKDVNVTAPADVENTDGVHVG 850
K P + + V G+ + N++ H+ + S + + + + AP D NTDG+HV
Sbjct: 84 KGAPTAVTIDSCRGVRVRGLTIQNAQQMHLTVSRSRGVRLDGMAIQAPGDSPNTDGIHVA 263
Query: 849 GSSKISILNSTIGTGDDCVSIGPGCNGVLVDSITCGPGQGISVGCLGRYKDEKDVSDITV 670
S+ ++I + IGTGDDCVSI V + I C PG GIS+G LG+ V +T+
Sbjct: 264 ESTAVTITGARIGTGDDCVSISNASFAVKMKGIVCDPGHGISIGSLGQGGSYAAVEGVTL 443
Query: 669 RNCVLKNTTNGVRIKS 622
+ NGVRIK+
Sbjct: 444 DGARIARAQNGVRIKT 491
Score = 31.3 bits (62), Expect(2) = 4e-027
Identities = 9/26 (34%), Positives = 19/26 (73%)
Frame = -2 / +3
Query: 588 HLTFENIRMEEVANPIVIDQYFCPQK 511
++ F N+ ++ V +PI+IDQ++C +
Sbjct: 519 NVRFTNVLVDGVDHPIIIDQFYCDSR 596
>gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_B18 5', mRNA sequence
Length = 431
Score = 121 bits (258), Expect = 1e-026
Identities = 46/91 (50%), Positives = 59/91 (64%)
Frame = -2 / +1
Query: 1368 FDISKLGATSDGKTDCTKAVQDAWTSACEATGSATVVIPKGDYLVGPLNFTGPCKGSNIA 1189
+DI KLGA DGKTD TKAV++AW SAC TG T+VIPKGD+L G LNFTGPCKG +
Sbjct: 157 YDIIKLGAKPDGKTDSTKAVEEAWASACGGTGKQTIVIPKGDFLTGALNFTGPCKGDGVT 336
Query: 1188 IQXXXXXXXXXXXDKYTASWIELSHVNNIGI 1096
I+ + ++WIE+ + N+ I
Sbjct: 337 IKLEGNLLASNDLSMFPSNWIEIMRIKNLVI 429
Score = 105 bits (223), Expect = 9e-022
Identities = 50/81 (61%), Positives = 55/81 (67%)
Frame = +2 / -1
Query: 1124 SIQLAVYLSRSFDPSRLPSSWMAMLLPLHGPVKLRGPTR*SPLGITTVALPVASHADVHA 1303
SIQL + RS + S+LPSS M PLHGPVK R P R SPLG+T V LPV HA+ HA
Sbjct: 401 SIQLDGNMDRSLEASKLPSSLMVTPSPLHGPVKFRAPVRKSPLGMTIVCLPVPPHAEAHA 222
Query: 1304 SWTAFVQSVLPSLVAPSLEMS 1366
S TA V SVLPS APSL MS
Sbjct: 221 SSTALVLSVLPSGFAPSLMMS 159
>gb|CA030305.1|CA030305 HX06K19r HX Hordeum vulgare subsp. vulgare cDNA clone HX06K19
5-PRIME, mRNA sequence
Length = 546
Score = 73.9 bits (155), Expect(2) = 9e-025
Identities = 31/81 (38%), Positives = 50/81 (61%)
Frame = -2 / +1
Query: 594 ASHLTFENIRMEEVANPIVIDQYFCPQKVCPGKRSNSSHVSVKDVTFRNITGTSSTPEAI 415
A + F N+ M+ V+NPI+IDQY + G + + V+V+ + + NI GTS++ A+
Sbjct: 229 ARKVKFANVVMKNVSNPIIIDQYRSEHPIPCGSTAATRTVAVEKIDYVNIAGTSASKRAV 408
Query: 414 SLLCSETQPCSGVSLIDVNVE 352
+ CS+ PC VSL DVN++
Sbjct: 409 TFSCSDVVPCRQVSLKDVNLK 471
Score = 60.6 bits (126), Expect(2) = 9e-025
Identities = 26/65 (40%), Positives = 36/65 (55%)
Frame = -2 / +1
Query: 807 GDDCVSIGPGCNGVLVDSITCGPGQGISVGCLGRYKDEKDVSDITVRNCVLKNTTNGVRI 628
G CVS+ C V + I+CGPG GIS+G +G + I V + NTTNG+ I
Sbjct: 22 GGACVSMAGNCTDVRLRYISCGPGNGISIGSIGETPVADRLEKIEVDTMFIANTTNGLLI 201
Query: 627 KSYVD 613
K++ D
Sbjct: 202 KTWQD 216
>gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachis EST library HVcDNA0015 (normal)
Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0008L17f,
mRNA sequence
Length = 458
Score = 105 bits (224), Expect = 6e-022
Identities = 42/104 (40%), Positives = 61/104 (58%)
Frame = -2 / +1
Query: 1020 PNTLVLFHVINATVAGIKLLNSKFFHINIDNSESITVKDVNVTAPADVENTDGVHVGGSS 841
P L F N TV G+K+ NS FH D+ + V +++ +PA NTDG+HV +
Sbjct: 52 PVALRFFMSNNVTVQGLKVQNSPEFHFRFDSCRGVRVYSLSIISPALSPNTDGIHVENTQ 231
Query: 840 KISILNSTIGTGDDCVSIGPGCNGVLVDSITCGPGQGISVGCLG 709
+ I N+ + GD+CV IG G V ++++TCGPG GI +G LG
Sbjct: 232 DVLITNTVVSNGDNCVCIGAGTLNVHIENVTCGPGHGIIIGSLG 363
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 652,413,406
Number of Sequences: 312970
Number of extensions: 11022980
Number of successful extensions: 151895
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 19056590664
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)