TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3696537.2.1
(592 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF625611.2|BF625611 HVSMEa0011O14f Hordeum vulgare seedl... 251 2e-066
gb|CD662384.1|CD662384 UCRHV18_02aa05_b1 Drought-stressed D... 251 2e-066
gb|AV928554.1|AV928554 AV928554 K. Sato unpublished cDNA li... 238 2e-062
gb|BG369930.2|BG369930 HVSMEi0026J11f Hordeum vulgare 20 DA... 217 5e-056
gb|BQ135330.1|BQ135330 LP10034 Lemma/palea-enriched cDNA li... 213 6e-055
gb|AW983496.2|AW983496 HVSMEg0010N10f Hordeum vulgare pre-a... 194 6e-055
gb|BF255168.2|BF255168 HVSMEf0006C06f Hordeum vulgare seedl... 171 2e-042
gb|CD663353.1|CD663353 UCRHV18_05ah10_b1 Drought-stressed D... 114 6e-041
gb|AV936335.1|AV936335 AV936335 K. Sato unpublished cDNA li... 114 6e-041
gb|CB880964.1|CB880964 HM08F20w HM Hordeum vulgare subsp. v... 114 6e-041
gb|CB882449.1|CB882449 HL01O07w HL Hordeum vulgare subsp. v... 114 6e-041
gb|CV059125.1|CV059125 BNEL44f5 Barley EST endosperm librar... 111 3e-038
gb|BF629044.2|BF629044 HVSMEb0009K16f Hordeum vulgare seedl... 138 3e-032
gb|BJ467124.1|BJ467124 BJ467124 K. Sato unpublished cDNA li... 85 4e-030
gb|BQ467017.1|BQ467017 HS02E20r HS Hordeum vulgare subsp. v... 123 6e-028
gb|BG343770.2|BG343770 HVSMEg0006L08f Hordeum vulgare pre-a... 88 2e-027
gb|BQ466698.1|BQ466698 HS01E20T HS Hordeum vulgare subsp. v... 119 2e-026
gb|BQ664207.1|BQ664207 HV02F10u HV Hordeum vulgare subsp. v... 73 8e-026
gb|CA010449.1|CA010449 HT09N07u HT Hordeum vulgare subsp. v... 73 4e-025
gb|AL505025.1|AL505025 AL505025 Hordeum vulgare Barke roots... 112 2e-024
gb|AV928420.1|AV928420 AV928420 K. Sato unpublished cDNA li... 73 7e-024
gb|AV935894.1|AV935894 AV935894 K. Sato unpublished cDNA li... 84 9e-024
gb|BJ475980.1|BJ475980 BJ475980 K. Sato unpublished cDNA li... 84 9e-024
gb|AV920842.1|AV920842 AV920842 K. Sato unpublished cDNA li... 81 2e-022
gb|CA001227.1|CA001227 HS18N03u HS Hordeum vulgare subsp. v... 104 5e-022
gb|AV911026.1|AV911026 AV911026 K. Sato unpublished cDNA li... 73 6e-021
>gb|BF625611.2|BF625611 HVSMEa0011O14f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011O14f, mRNA sequence
Length = 638
Score = 251 bits (543), Expect = 2e-066
Identities = 93/117 (79%), Positives = 101/117 (86%)
Frame = -2 / +2
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MH NCPVC EYLFDS ISVLHCGHTIHLECL EMR H FSCPVC RSAC+M+D WQK
Sbjct: 179 MHQNCPVCYEYLFDSTMDISVLHCGHTIHLECLNEMRVHHHFSCPVCSRSACDMTDAWQK 358
Query: 411 LDQQVAASPMPAIYQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQTRGDPA 241
LDQ+VAA+PMP YQKKMVWILCNDCG TS+V+FH+LA KCPGCSSYNTR+TRG PA
Sbjct: 359 LDQEVAATPMPEFYQKKMVWILCNDCGATSSVRFHVLAQKCPGCSSYNTRETRGGPA 529
Score = 177 bits (380), Expect = 6e-044
Identities = 79/117 (67%), Positives = 87/117 (74%)
Frame = +2 / -3
Query: 242 AGSPLVCRVL*ELHPGHLCAKMWNCTLDVTPQSLQRIHTIFF**IAGIGDAATC*SSFCQ 421
AG PLV VL*ELHPGH CA+ W TLDV PQSLQRIHTIFF * +G+G AAT SSFC
Sbjct: 528 AGPPLVSLVL*ELHPGHFCARTWKRTLDVAPQSLQRIHTIFFW*NSGMGVAATSWSSFCH 349
Query: 422 MSDMLQADLRHTGHENCWCALIS*RHSRWMVCPQWSTLIAFIESNRYSMQTGQL*CI 592
S M QAD TGHE LIS RHS+W+VCPQ STL++ +ESNRYS QTGQ *CI
Sbjct: 348 ASVMSQADREQTGHEKW**TLISFRHSKWIVCPQCSTLMSMVESNRYS*QTGQF*CI 178
Score = 161 bits (346), Expect = 3e-039
Identities = 70/118 (59%), Positives = 89/118 (75%)
Frame = +1 / -2
Query: 238 CGWIAPRLPGVVRAASRALVRQDVELHVGRHATVVAKDPYHLLLIDRWHRGRCNLLIQLL 417
C APRLPGVVRAA L+RQDVE H R VVA+DPYHLLL++ WH +LL++LL
Sbjct: 532 CSRAAPRLPGVVRAAPGTLLRQDVEAHARRRPAVVAEDPYHLLLVELWHGCGGDLLVELL 353
Query: 418 PDVGHVAGRPQAHRA*KLLVRSHLVKAFQVDGVSAVEHADSFHRVE*ILHADRAVVMH 591
P +GHVAGRP+A RA ++++ HLV+A Q+D V AV++AD RV+*IL ADRAV+MH
Sbjct: 352 PRIGHVAGRPRADRAREVVMNPHLVQALQMDRVPAVQYADVHGRVK*ILVADRAVLMH 179
Score = 127 bits (272), Expect = 5e-029
Identities = 61/114 (53%), Positives = 75/114 (65%)
Frame = -1 / +1
Query: 589 ASQLPCLHGVSIRLDESYQRAPLRTHHPPGMPLRDESAPAVFMPGVPEVCLQHVRHLAKA 410
AS+LPCL VSI LD +QR LR H P G+P RDE + + +PG+ V L+H R +A+A
Sbjct: 181 ASKLPCLLRVSI*LDHGHQRTALRAHDPFGVPERDEGSSPLLVPGLLAVGLRHDRCVAEA 360
Query: 409 GSAGCSVPDASDLSEEDGMDPLQRLWRDVQRAVPHLGAQVPWMQLLQHPADEGR 248
G A L EEDGMDPLQRL DV+RA+P GA+V +QLLQH DEGR
Sbjct: 361 RPGGRRHTHARVLPEEDGMDPLQRLRGDVERALPRPGAKVSRVQLLQHQGDEGR 522
Score = 111 bits (237), Expect = 3e-024
Identities = 55/114 (48%), Positives = 64/114 (56%)
Frame = +3 / -1
Query: 249 RPSSAGCCKSCIQGTCAPRCGTARWTSRHSRCKGSIPSSSDRSLASGTLQPADPAFARCR 428
RPSS CCKSC + T AP G+AR TS SRC+GSIPSSS R+LA +P A A R
Sbjct: 521 RPSSPWCCKSCTRDTFAPGRGSARSTSPRSRCRGSIPSSSGRTLAWVWRRPPGRASATHR 342
Query: 429 TCCRQTSGTPGMKTAGALSSRKGIPGGWCVXXXXXXXXXXXXIDTPCRQGSCDA 590
+C R T+ PG ++ SSR G P G C IDT RQGS DA
Sbjct: 341 SCRRPTASRPGTRSGDEPSSRSGTPNGSCARSAVR*CPWSSQIDTRSRQGSFDA 180
Score = 63.4 bits (132), Expect(2) = 1e-021
Identities = 26/37 (70%), Positives = 27/37 (72%)
Frame = -3 / +3
Query: 359 WYGSFATTVA*RPTCSSTSWRTSALDAALTTPGRRGA 249
WYGS ATT R C+STSWR S AALTTPGRRGA
Sbjct: 411 WYGSSATTAGRRRACASTSWRKSVPGAALTTPGRRGA 521
Score = 59.3 bits (123), Expect(2) = 1e-021
Identities = 33/60 (55%), Positives = 37/60 (61%)
Frame = -3 / +3
Query: 590 CITTALSAWSIYSTR*KLSACSTADTPSTWNAFTR*ERTSSFHARCA*GLPATCPTSGKS 411
CI TALSA SIY TR SA TA T S W+A TR* ++ AR A G PAT P G+S
Sbjct: 180 CIKTALSATSIYLTRPWTSAYCTAGTRSIWSA*TR*GFITTSRARSARGRPAT*PMRGRS 359
>gb|CD662384.1|CD662384 UCRHV18_02aa05_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_02aa05, mRNA sequence
Length = 896
Score = 251 bits (543), Expect = 2e-066
Identities = 93/117 (79%), Positives = 101/117 (86%)
Frame = -2 / -3
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MH NCPVC EYLFDS ISVLHCGHTIHLECL EMR H FSCPVC RSAC+M+D WQK
Sbjct: 792 MHQNCPVCYEYLFDSTMDISVLHCGHTIHLECLNEMRVHHHFSCPVCSRSACDMTDAWQK 613
Query: 411 LDQQVAASPMPAIYQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQTRGDPA 241
LDQ+VAA+PMP YQKKMVWILCNDCG TS+V+FH+LA KCPGCSSYNTR+TRG PA
Sbjct: 612 LDQEVAATPMPEFYQKKMVWILCNDCGATSSVRFHVLAQKCPGCSSYNTRETRGGPA 442
Score = 177 bits (380), Expect = 6e-044
Identities = 79/117 (67%), Positives = 87/117 (74%)
Frame = +2 / +2
Query: 242 AGSPLVCRVL*ELHPGHLCAKMWNCTLDVTPQSLQRIHTIFF**IAGIGDAATC*SSFCQ 421
AG PLV VL*ELHPGH CA+ W TLDV PQSLQRIHTIFF * +G+G AAT SSFC
Sbjct: 443 AGPPLVSLVL*ELHPGHFCARTWKRTLDVAPQSLQRIHTIFFW*NSGMGVAATSWSSFCH 622
Query: 422 MSDMLQADLRHTGHENCWCALIS*RHSRWMVCPQWSTLIAFIESNRYSMQTGQL*CI 592
S M QAD TGHE LIS RHS+W+VCPQ STL++ +ESNRYS QTGQ *CI
Sbjct: 623 ASVMSQADREQTGHEKW**TLISFRHSKWIVCPQCSTLMSMVESNRYS*QTGQF*CI 793
Score = 159 bits (341), Expect = 2e-038
Identities = 69/114 (60%), Positives = 88/114 (77%)
Frame = +1 / +1
Query: 250 APRLPGVVRAASRALVRQDVELHVGRHATVVAKDPYHLLLIDRWHRGRCNLLIQLLPDVG 429
APRLPGVVRAA L+RQDVE H R VVA+DPYHLLL++ WH +LL++LLP +G
Sbjct: 451 APRLPGVVRAAPGTLLRQDVEAHARRRPAVVAEDPYHLLLVELWHGCGGDLLVELLPRIG 630
Query: 430 HVAGRPQAHRA*KLLVRSHLVKAFQVDGVSAVEHADSFHRVE*ILHADRAVVMH 591
HVAGRP+A RA ++++ HLV+A Q+D V AV++AD RV+*IL ADRAV+MH
Sbjct: 631 HVAGRPRADRAREVVMNPHLVQALQMDRVPAVQYADVHGRVK*ILVADRAVLMH 792
Score = 127 bits (272), Expect = 5e-029
Identities = 61/114 (53%), Positives = 75/114 (65%)
Frame = -1 / -2
Query: 589 ASQLPCLHGVSIRLDESYQRAPLRTHHPPGMPLRDESAPAVFMPGVPEVCLQHVRHLAKA 410
AS+LPCL VSI LD +QR LR H P G+P RDE + + +PG+ V L+H R +A+A
Sbjct: 790 ASKLPCLLRVSI*LDHGHQRTALRAHDPFGVPERDEGSSPLLVPGLLAVGLRHDRCVAEA 611
Query: 409 GSAGCSVPDASDLSEEDGMDPLQRLWRDVQRAVPHLGAQVPWMQLLQHPADEGR 248
G A L EEDGMDPLQRL DV+RA+P GA+V +QLLQH DEGR
Sbjct: 610 RPGGRRHTHARVLPEEDGMDPLQRLRGDVERALPRPGAKVSRVQLLQHQGDEGR 449
Score = 111 bits (237), Expect = 3e-024
Identities = 55/114 (48%), Positives = 64/114 (56%)
Frame = +3 / +3
Query: 249 RPSSAGCCKSCIQGTCAPRCGTARWTSRHSRCKGSIPSSSDRSLASGTLQPADPAFARCR 428
RPSS CCKSC + T AP G+AR TS SRC+GSIPSSS R+LA +P A A R
Sbjct: 450 RPSSPWCCKSCTRDTFAPGRGSARSTSPRSRCRGSIPSSSGRTLAWVWRRPPGRASATHR 629
Query: 429 TCCRQTSGTPGMKTAGALSSRKGIPGGWCVXXXXXXXXXXXXIDTPCRQGSCDA 590
+C R T+ PG ++ SSR G P G C IDT RQGS DA
Sbjct: 630 SCRRPTASRPGTRSGDEPSSRSGTPNGSCARSAVR*CPWSSQIDTRSRQGSFDA 791
Score = 63.4 bits (132), Expect(2) = 1e-021
Identities = 26/37 (70%), Positives = 27/37 (72%)
Frame = -3 / -1
Query: 359 WYGSFATTVA*RPTCSSTSWRTSALDAALTTPGRRGA 249
WYGS ATT R C+STSWR S AALTTPGRRGA
Sbjct: 560 WYGSSATTAGRRRACASTSWRKSVPGAALTTPGRRGA 450
Score = 59.3 bits (123), Expect(2) = 1e-021
Identities = 33/60 (55%), Positives = 37/60 (61%)
Frame = -3 / -1
Query: 590 CITTALSAWSIYSTR*KLSACSTADTPSTWNAFTR*ERTSSFHARCA*GLPATCPTSGKS 411
CI TALSA SIY TR SA TA T S W+A TR* ++ AR A G PAT P G+S
Sbjct: 791 CIKTALSATSIYLTRPWTSAYCTAGTRSIWSA*TR*GFITTSRARSARGRPAT*PMRGRS 612
>gb|AV928554.1|AV928554 AV928554 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdn20 3', mRNA sequence
Length = 751
Score = 238 bits (515), Expect = 2e-062
Identities = 89/112 (79%), Positives = 97/112 (86%)
Frame = -2 / -3
Query: 576 PVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQV 397
PVC EYLFDS ISVLHCGHTIHLECL EMR H FSCPVC RSAC+M+D WQKLDQ+V
Sbjct: 731 PVCYEYLFDSTMDISVLHCGHTIHLECLNEMRVHHHFSCPVCSRSACDMTDAWQKLDQEV 552
Query: 396 AASPMPAIYQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQTRGDPA 241
AA+PMP YQKKMVWILCNDCG TS+V+FH+LA KCPGCSSYNTR+TRG PA
Sbjct: 551 AATPMPEFYQKKMVWILCNDCGATSSVRFHVLAQKCPGCSSYNTRETRGGPA 396
Score = 168 bits (361), Expect = 3e-041
Identities = 75/112 (66%), Positives = 83/112 (74%)
Frame = +2 / +1
Query: 242 AGSPLVCRVL*ELHPGHLCAKMWNCTLDVTPQSLQRIHTIFF**IAGIGDAATC*SSFCQ 421
AG PLV VL*ELHPGH CA+ W TLDV PQSLQRIHTIFF * +G+G AAT SSFC
Sbjct: 397 AGPPLVSLVL*ELHPGHFCARTWKRTLDVAPQSLQRIHTIFFW*NSGMGVAATSWSSFCH 576
Query: 422 MSDMLQADLRHTGHENCWCALIS*RHSRWMVCPQWSTLIAFIESNRYSMQTG 577
S M QAD TGHE LIS RHS+W+VCPQ STL++ +ESNRYS QTG
Sbjct: 577 ASVMSQADREQTGHEKW**TLISFRHSKWIVCPQCSTLMSMVESNRYS*QTG 732
Score = 149 bits (319), Expect = 2e-035
Identities = 65/109 (59%), Positives = 83/109 (76%)
Frame = +1 / +3
Query: 250 APRLPGVVRAASRALVRQDVELHVGRHATVVAKDPYHLLLIDRWHRGRCNLLIQLLPDVG 429
APRLPGVVRAA L+RQDVE H R VVA+DPYHLLL++ WH +LL++LLP +G
Sbjct: 405 APRLPGVVRAAPGTLLRQDVEAHARRRPAVVAEDPYHLLLVELWHGCGGDLLVELLPRIG 584
Query: 430 HVAGRPQAHRA*KLLVRSHLVKAFQVDGVSAVEHADSFHRVE*ILHADR 576
HVAGRP+A RA ++++ HLV+A Q+D V AV++AD RV+*IL ADR
Sbjct: 585 HVAGRPRADRAREVVMNPHLVQALQMDRVPAVQYADVHGRVK*ILVADR 731
Score = 121 bits (259), Expect = 3e-027
Identities = 58/110 (52%), Positives = 71/110 (64%)
Frame = -1 / -2
Query: 577 PCLHGVSIRLDESYQRAPLRTHHPPGMPLRDESAPAVFMPGVPEVCLQHVRHLAKAGSAG 398
PCL VSI LD +QR LR H P G+P RDE + + +PG+ V L+H R +A+A G
Sbjct: 732 PCLLRVSI*LDHGHQRTALRAHDPFGVPERDEGSSPLLVPGLLAVGLRHDRCVAEARPGG 553
Query: 397 CSVPDASDLSEEDGMDPLQRLWRDVQRAVPHLGAQVPWMQLLQHPADEGR 248
A L EEDGMDPLQRL DV+RA+P GA+V +QLLQH DEGR
Sbjct: 552 RRHTHARVLPEEDGMDPLQRLRGDVERALPRPGAKVSRVQLLQHQGDEGR 403
Score = 105 bits (225), Expect = 2e-022
Identities = 52/110 (47%), Positives = 61/110 (55%)
Frame = +3 / +2
Query: 249 RPSSAGCCKSCIQGTCAPRCGTARWTSRHSRCKGSIPSSSDRSLASGTLQPADPAFARCR 428
RPSS CCKSC + T AP G+AR TS SRC+GSIPSSS R+LA +P A A R
Sbjct: 404 RPSSPWCCKSCTRDTFAPGRGSARSTSPRSRCRGSIPSSSGRTLAWVWRRPPGRASATHR 583
Query: 429 TCCRQTSGTPGMKTAGALSSRKGIPGGWCVXXXXXXXXXXXXIDTPCRQG 578
+C R T+ PG ++ SSR G P G C IDT RQG
Sbjct: 584 SCRRPTASRPGTRSGDEPSSRSGTPNGSCARSAVR*CPWSSQIDTRSRQG 733
>gb|BG369930.2|BG369930 HVSMEi0026J11f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0026J11f, mRNA sequence
Length = 682
Score = 217 bits (468), Expect = 5e-056
Identities = 80/105 (76%), Positives = 87/105 (82%)
Frame = -2 / +3
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHHNCPVC EYLFDS ISVLHCGHTIHLECL EMR H FSCPV R AC+M+D WQK
Sbjct: 366 MHHNCPVCYEYLFDSTMDISVLHCGHTIHLECLNEMRVHHHFSCPV*SRPACDMTDAWQK 545
Query: 411 LDQQVAASPMPAIYQKKMVWILCNDCGVTSNVQFHILAHKCPGCS 277
LDQ+VA +PMP YQKKMVWILC DCG TS+V+FH+LA KCPGCS
Sbjct: 546 LDQEVAVTPMPEFYQKKMVWILCKDCGATSSVRFHVLAQKCPGCS 680
Score = 159 bits (341), Expect = 2e-038
Identities = 70/106 (66%), Positives = 78/106 (73%)
Frame = +2 / -1
Query: 275 ELHPGHLCAKMWNCTLDVTPQSLQRIHTIFF**IAGIGDAATC*SSFCQMSDMLQADLRH 454
ELHPGH CA+ W TLDV PQSLQRIHTIFF * +G+G AT SSFC S M QA
Sbjct: 682 ELHPGHFCARTWKRTLDVAPQSLQRIHTIFFW*NSGMGVTATSWSSFCHASVMSQAGRDQ 503
Query: 455 TGHENCWCALIS*RHSRWMVCPQWSTLIAFIESNRYSMQTGQL*CI 592
TGHE LIS RHS+W+VCPQ STL++ +ESNRYS QTGQL*CI
Sbjct: 502 TGHEKW**TLISFRHSKWIVCPQCSTLMSMVESNRYS*QTGQL*CI 365
Score = 133 bits (284), Expect = 1e-030
Identities = 58/105 (55%), Positives = 77/105 (73%)
Frame = +1 / -3
Query: 277 AASRALVRQDVELHVGRHATVVAKDPYHLLLIDRWHRGRCNLLIQLLPDVGHVAGRPQAH 456
AA +RQDVE H R V A+DPYHLLL++ WH +LL++LLP +GHVAGRP++
Sbjct: 680 AAPGTFLRQDVEAHA*RRPAVFAEDPYHLLLVELWHGCDGHLLVELLPRIGHVAGRPRSD 501
Query: 457 RA*KLLVRSHLVKAFQVDGVSAVEHADSFHRVE*ILHADRAVVMH 591
RA ++++ HLV+A Q+D V AV++AD RV+*IL ADRAVVMH
Sbjct: 500 RAREVVMNPHLVQALQMDRVPAVQYADVHGRVK*ILVADRAVVMH 366
Score = 113 bits (242), Expect = 7e-025
Identities = 54/105 (51%), Positives = 68/105 (64%)
Frame = -1 / +2
Query: 589 ASQLPCLHGVSIRLDESYQRAPLRTHHPPGMPLRDESAPAVFMPGVPEVCLQHVRHLAKA 410
ASQLPCL VSI LD +QR LR H P G+P RDE + + +PG+ CL+H R +A+A
Sbjct: 368 ASQLPCLLRVSI*LDHGHQRTALRAHDPFGVPERDEGSSPLLVPGLIAACLRHDRCVAEA 547
Query: 409 GSAGCSVPDASDLSEEDGMDPLQRLWRDVQRAVPHLGAQVPWMQL 275
G A L EEDGMDPLQRL DV+RA+P GA++ +QL
Sbjct: 548 RPGGGRHTHARVLPEEDGMDPLQRLRGDVKRALPRPGAKMSRVQL 682
>gb|BQ135330.1|BQ135330 LP10034 Lemma/palea-enriched cDNA library from elongation stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 1-34,
mRNA sequence
Length = 338
Score = 213 bits (460), Expect = 6e-055
Identities = 78/97 (80%), Positives = 86/97 (88%)
Frame = -2 / -1
Query: 531 VLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQVAASPMPAIYQKKMVW 352
VLHCGHTIHLECL EMR H FSCPVC RSAC+M+D WQKLDQ+VAA+PMP YQKKMVW
Sbjct: 338 VLHCGHTIHLECLNEMRVHHHFSCPVCSRSACDMTDAWQKLDQEVAATPMPEFYQKKMVW 159
Query: 351 ILCNDCGVTSNVQFHILAHKCPGCSSYNTRQTRGDPA 241
ILCNDCG TS+V+FH+LA KCPGCSSYNTR+TRG PA
Sbjct: 158 ILCNDCGATSSVRFHVLAQKCPGCSSYNTRETRGGPA 48
Score = 142 bits (304), Expect = 2e-033
Identities = 64/96 (66%), Positives = 69/96 (71%)
Frame = +2 / +1
Query: 242 AGSPLVCRVL*ELHPGHLCAKMWNCTLDVTPQSLQRIHTIFF**IAGIGDAATC*SSFCQ 421
AG PLV VL*ELHPGH CA+ W TLDV PQSLQRIHTIFF * +G+G AAT SSFC
Sbjct: 49 AGPPLVSLVL*ELHPGHFCARTWKRTLDVAPQSLQRIHTIFFW*NSGMGVAATSWSSFCH 228
Query: 422 MSDMLQADLRHTGHENCWCALIS*RHSRWMVCPQWS 529
S M QAD TGHE LIS RHS+W+VCPQ S
Sbjct: 229 ASVMSQADREQTGHEKW**TLISFRHSKWIVCPQCS 336
Score = 132 bits (283), Expect = 2e-030
Identities = 55/94 (58%), Positives = 72/94 (76%)
Frame = +1 / +3
Query: 250 APRLPGVVRAASRALVRQDVELHVGRHATVVAKDPYHLLLIDRWHRGRCNLLIQLLPDVG 429
APRLPGVVRAA L+RQDVE H R VVA+DPYHLLL++ WH +LL++LLP +G
Sbjct: 57 APRLPGVVRAAPGTLLRQDVEAHARRRPAVVAEDPYHLLLVELWHGCGGDLLVELLPRIG 236
Query: 430 HVAGRPQAHRA*KLLVRSHLVKAFQVDGVSAVEH 531
HVAGRP+A RA ++++ HLV+A Q+D V AV++
Sbjct: 237 HVAGRPRADRAREVVMNPHLVQALQMDRVPAVQY 338
Score = 101 bits (215), Expect = 4e-021
Identities = 48/92 (52%), Positives = 60/92 (65%)
Frame = -1 / -3
Query: 523 LRTHHPPGMPLRDESAPAVFMPGVPEVCLQHVRHLAKAGSAGCSVPDASDLSEEDGMDPL 344
LR H P G+P RDE + + +PG+ V L+H R +A+A G A L EEDGMDPL
Sbjct: 330 LRAHDPFGVPERDEGSSPLLVPGLLAVGLRHDRCVAEARPGGRRHTHARVLPEEDGMDPL 151
Query: 343 QRLWRDVQRAVPHLGAQVPWMQLLQHPADEGR 248
QRL DV+RA+P GA+V +QLLQH DEGR
Sbjct: 150 QRLRGDVERALPRPGAKVSRVQLLQHQGDEGR 55
>gb|AW983496.2|AW983496 HVSMEg0010N10f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0010N10f, mRNA sequence
Length = 884
Score = 194 bits (419), Expect(2) = 6e-055
Identities = 73/96 (76%), Positives = 81/96 (84%)
Frame = -2 / +2
Query: 537 ISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQVAASPMPAIYQKKM 358
ISVLHCGHTIHLECL EMR H FS PVC RSAC+M+D WQKLDQ+VAA+PMP YQKKM
Sbjct: 164 ISVLHCGHTIHLECLNEMRVHHHFSGPVCSRSACDMTDAWQKLDQEVAATPMPEFYQKKM 343
Query: 357 VWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQTRG 250
VWILCNDCG TS+V+FH+LA KCPGCS YNT +G
Sbjct: 344 VWILCNDCGATSSVRFHVLAQKCPGCSFYNTPGEQG 451
Score = 131 bits (280), Expect(2) = 5e-034
Identities = 59/92 (64%), Positives = 67/92 (72%)
Frame = +2 / -3
Query: 266 VL*ELHPGHLCAKMWNCTLDVTPQSLQRIHTIFF**IAGIGDAATC*SSFCQMSDMLQAD 445
VL*+LHPGH CA+ W TLDV PQSLQRIHTIFF * +G+G AAT SSFC S M QAD
Sbjct: 435 VL*KLHPGHFCARTWKRTLDVAPQSLQRIHTIFFW*NSGMGVAATSWSSFCHASVMSQAD 256
Query: 446 LRHTGHENCWCALIS*RHSRWMVCPQWSTLIA 541
TG E LIS RHS+W+VCPQ STL++
Sbjct: 255 REQTGPEKW**TLISFRHSKWIVCPQCSTLMS 160
Score = 121 bits (258), Expect(2) = 2e-029
Identities = 50/91 (54%), Positives = 69/91 (75%)
Frame = +1 / -2
Query: 265 GVVRAASRALVRQDVELHVGRHATVVAKDPYHLLLIDRWHRGRCNLLIQLLPDVGHVAGR 444
GVV+AA L+RQ+VE H R VVA+DPYHLLL++ WH +LL++LLP +GHVAGR
Sbjct: 436 GVVKAAPGTLLRQNVEAHARRRPAVVAEDPYHLLLVELWHGCGGDLLVELLPRIGHVAGR 257
Query: 445 PQAHRA*KLLVRSHLVKAFQVDGVSAVEHAD 537
P+A RA ++++ HLV+A Q+D V AV++AD
Sbjct: 256 PRADRAREVVMNPHLVQALQMDRVPAVQYAD 164
Score = 99.6 bits (211), Expect(2) = 4e-023
Identities = 48/96 (50%), Positives = 60/96 (62%)
Frame = -1 / +1
Query: 538 YQRAPLRTHHPPGMPLRDESAPAVFMPGVPEVCLQHVRHLAKAGSAGCSVPDASDLSEED 359
+QR LR H P G+P RDE + + PG+ V L+H R +A+A G A L EED
Sbjct: 163 HQRTALRAHDPFGVPERDEGSSPLLGPGLLAVGLRHDRCVAEARPGGRRHTHARVLPEED 342
Query: 358 GMDPLQRLWRDVQRAVPHLGAQVPWMQLLQHPADEG 251
GMDPLQRL DV+RA+P GA+V +QLLQHP G
Sbjct: 343 GMDPLQRLRGDVERALPRSGAKVSRVQLLQHPRRTG 450
Score = 39.1 bits (79), Expect(2) = 6e-055
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = -2 / +1
Query: 591 MHHNCPVCMEYLFDS 547
MH NCPVC EYLFDS
Sbjct: 109 MHQNCPVCYEYLFDS 153
Score = 32.7 bits (65), Expect(2) = 5e-034
Identities = 13/16 (81%), Positives = 14/16 (87%)
Frame = +2 / -1
Query: 545 IESNRYSMQTGQL*CI 592
+ESNRYS QTGQ *CI
Sbjct: 155 VESNRYS*QTGQF*CI 108
Score = 27.6 bits (54), Expect(2) = 2e-029
Identities = 13/18 (72%), Positives = 15/18 (83%)
Frame = +1 / -3
Query: 538 SFHRVE*ILHADRAVVMH 591
S RV+*IL ADRAV+MH
Sbjct: 162 SHGRVK*ILVADRAVLMH 109
Score = 27.6 bits (54), Expect(2) = 4e-023
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -3 / +2
Query: 590 CITTALSAWSIYSTR 546
CI TALSA SIY TR
Sbjct: 110 CIKTALSATSIYLTR 154
>gb|BF255168.2|BF255168 HVSMEf0006C06f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006C06f, mRNA sequence
Length = 605
Score = 171 bits (369), Expect = 2e-042
Identities = 62/79 (78%), Positives = 70/79 (88%)
Frame = -2 / +1
Query: 477 HQQFSCPVCLRSACNMSDIWQKLDQQVAASPMPAIYQKKMVWILCNDCGVTSNVQFHILA 298
H FSCPVC RSAC+M+D WQKLDQ+VAA+PMP YQKKMVWILCNDCG TS+V+FH+LA
Sbjct: 4 HHHFSCPVCSRSACDMTDAWQKLDQEVAATPMPEFYQKKMVWILCNDCGATSSVRFHVLA 183
Query: 297 HKCPGCSSYNTRQTRGDPA 241
KCPGCSSYNTR+TRG PA
Sbjct: 184 QKCPGCSSYNTRETRGGPA 240
Score = 116 bits (248), Expect = 1e-025
Identities = 52/75 (69%), Positives = 55/75 (73%)
Frame = +2 / -1
Query: 242 AGSPLVCRVL*ELHPGHLCAKMWNCTLDVTPQSLQRIHTIFF**IAGIGDAATC*SSFCQ 421
AG PLV VL*ELHPGH CA+ W TLDV PQSLQRIHTIFF * +G+G AAT SSFC
Sbjct: 239 AGPPLVSLVL*ELHPGHFCARTWKRTLDVAPQSLQRIHTIFFW*NSGMGVAATSWSSFCH 60
Query: 422 MSDMLQADLRHTGHE 466
S M QAD TGHE
Sbjct: 59 ASVMSQADREQTGHE 15
Score = 111 bits (238), Expect = 2e-024
Identities = 46/71 (64%), Positives = 55/71 (77%)
Frame = +1 / -3
Query: 250 APRLPGVVRAASRALVRQDVELHVGRHATVVAKDPYHLLLIDRWHRGRCNLLIQLLPDVG 429
APRLPGVVRAA L+RQDVE H R VVA+DPYHLLL++ WH +LL++LLP +G
Sbjct: 231 APRLPGVVRAAPGTLLRQDVEAHARRRPAVVAEDPYHLLLVELWHGCGGDLLVELLPRIG 52
Query: 430 HVAGRPQAHRA 462
HVAGRP+A RA
Sbjct: 51 HVAGRPRADRA 19
>gb|CD663353.1|CD663353 UCRHV18_05ah10_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_05ah10, mRNA sequence
Length = 706
Score = 114 bits (243), Expect(2) = 6e-041
Identities = 38/66 (57%), Positives = 52/66 (78%)
Frame = -2 / -2
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHH+CP+C EYLF+S +SVL CGHTIH +CL EM+ H QF+CP+C +S C+MS W++
Sbjct: 588 MHHDCPICFEYLFESRNDVSVLPCGHTIHEKCLKEMKEHCQFACPLCSKSVCDMSKAWER 409
Query: 411 LDQQVA 394
LD ++A
Sbjct: 408 LDAELA 391
Score = 73.0 bits (153), Expect(2) = 6e-041
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / -2
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 375 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 262
>gb|AV936335.1|AV936335 AV936335 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal10p08 3', mRNA sequence
Length = 678
Score = 114 bits (243), Expect(2) = 6e-041
Identities = 38/66 (57%), Positives = 52/66 (78%)
Frame = -2 / -3
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHH+CP+C EYLF+S +SVL CGHTIH +CL EM+ H QF+CP+C +S C+MS W++
Sbjct: 511 MHHDCPICFEYLFESRNDVSVLPCGHTIHEKCLKEMKEHCQFACPLCSKSVCDMSKAWER 332
Query: 411 LDQQVA 394
LD ++A
Sbjct: 331 LDAELA 314
Score = 73.0 bits (153), Expect(2) = 6e-041
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / -3
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 298 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 185
>gb|CB880964.1|CB880964 HM08F20w HM Hordeum vulgare subsp. vulgare cDNA clone HM08F20
3-PRIME, mRNA sequence
Length = 663
Score = 114 bits (243), Expect(2) = 6e-041
Identities = 38/66 (57%), Positives = 52/66 (78%)
Frame = -2 / -2
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHH+CP+C EYLF+S +SVL CGHTIH +CL EM+ H QF+CP+C +S C+MS W++
Sbjct: 590 MHHDCPICFEYLFESRNDVSVLPCGHTIHEKCLKEMKEHCQFACPLCSKSVCDMSKAWER 411
Query: 411 LDQQVA 394
LD ++A
Sbjct: 410 LDAELA 393
Score = 73.0 bits (153), Expect(2) = 6e-041
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / -2
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 377 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 264
>gb|CB882449.1|CB882449 HL01O07w HL Hordeum vulgare subsp. vulgare cDNA clone HL01O07
3-PRIME, mRNA sequence
Length = 593
Score = 114 bits (243), Expect(2) = 6e-041
Identities = 38/66 (57%), Positives = 52/66 (78%)
Frame = -2 / +1
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHH+CP+C EYLF+S +SVL CGHTIH +CL EM+ H QF+CP+C +S C+MS W++
Sbjct: 166 MHHDCPICFEYLFESRNDVSVLPCGHTIHEKCLKEMKEHCQFACPLCSKSVCDMSKAWER 345
Query: 411 LDQQVA 394
LD ++A
Sbjct: 346 LDAELA 363
Score = 73.0 bits (153), Expect(2) = 6e-041
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / +1
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 379 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 492
>gb|CV059125.1|CV059125 BNEL44f5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL44f5 5' similar to zinc finger,
mRNA sequence
Length = 562
Score = 111 bits (238), Expect(2) = 3e-038
Identities = 37/78 (47%), Positives = 57/78 (73%)
Frame = -2 / +3
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
M NCP+C EYLFDS+K VL+CGHT+H++C EM H +++CP+C ++A +M+ W
Sbjct: 213 MRQNCPICYEYLFDSLKGTRVLNCGHTMHMDCFAEMVDHNKYTCPICSKTALDMTVHWGM 392
Query: 411 LDQQVAASPMPAIYQKKM 358
LDQ++ A+ MP +Y+ K+
Sbjct: 393 LDQEIEATIMPPVYRYKV 446
Score = 66.1 bits (138), Expect(2) = 3e-038
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = -2 / +3
Query: 357 VWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQT 256
VW+LCNDC S V FH++ HKC C+SYNTR T
Sbjct: 444 VWVLCNDCNKVSEVDFHVIGHKCSHCNSYNTRST 545
>gb|BF629044.2|BF629044 HVSMEb0009K16f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0009K16f, mRNA sequence
Length = 852
Score = 138 bits (295), Expect = 3e-032
Identities = 53/77 (68%), Positives = 59/77 (76%)
Frame = -2 / +3
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MH NCPVC EYLFDS ISVLHCGHTIHLECL EMR H FSCPVC RSAC+M+D QK
Sbjct: 576 MHQNCPVCYEYLFDSTMDISVLHCGHTIHLECLNEMRGHHHFSCPVCSRSACDMTDALQK 755
Query: 411 LDQQVAASPMPAIYQKK 361
LDQ+VA P + +K+
Sbjct: 756 LDQEVARPPSQILPKKR 806
>gb|BJ467124.1|BJ467124 BJ467124 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags36c23 3', mRNA sequence
Length = 672
Score = 84.9 bits (179), Expect(2) = 4e-030
Identities = 28/66 (42%), Positives = 47/66 (71%)
Frame = -2 / -1
Query: 555 FDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQVAASPMPA 376
FDS+K V +CGHT+H++C EM H +++CP+C ++A +M+ W LDQ++ A+ MP
Sbjct: 672 FDSLKGTRVXNCGHTMHMDCFAEMVDHNKYTCPICSKTALDMTVHWGMLDQEIEATIMPP 493
Query: 375 IYQKKM 358
+Y+ K+
Sbjct: 492 VYRYKV 475
Score = 66.1 bits (138), Expect(2) = 4e-030
Identities = 21/34 (61%), Positives = 25/34 (73%)
Frame = -2 / -1
Query: 357 VWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQT 256
VW+LCNDC S V FH++ HKC C+SYNTR T
Sbjct: 477 VWVLCNDCNKVSEVDFHVIGHKCSHCNSYNTRST 376
>gb|BQ467017.1|BQ467017 HS02E20r HS Hordeum vulgare subsp. vulgare cDNA clone HS02E20
5-PRIME, mRNA sequence
Length = 587
Score = 123 bits (264), Expect = 6e-028
Identities = 45/58 (77%), Positives = 47/58 (81%)
Frame = -2 / +2
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIW 418
MH NCPVC EYLFDS ISVLHCGHTIHLECL EMR H FSCPVC RSAC+M+D W
Sbjct: 413 MHQNCPVCYEYLFDSTMDISVLHCGHTIHLECLNEMRVHHHFSCPVCSRSACDMTDAW 586
>gb|BG343770.2|BG343770 HVSMEg0006L08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006L08f, mRNA sequence
Length = 996
Score = 87.7 bits (185), Expect(2) = 2e-027
Identities = 33/45 (73%), Positives = 33/45 (73%)
Frame = -2 / +2
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCP 457
MH NCPVC EYLFDS ISVLHCGHTIHLECL EMR H P
Sbjct: 545 MHQNCPVCYEYLFDSTMDISVLHCGHTIHLECLNEMRVHHHXLVP 679
Score = 54.2 bits (112), Expect(2) = 2e-027
Identities = 20/34 (58%), Positives = 25/34 (73%)
Frame = -2 / +3
Query: 465 SCPVCLRSACNMSDIWQKLDQQVAASPMPAIYQK 364
SCPVC RSAC+M+D W KLD VAA P ++ +K
Sbjct: 672 SCPVCSRSACDMTDAWXKLDXXVAAHPCQSLXKK 773
>gb|BQ466698.1|BQ466698 HS01E20T HS Hordeum vulgare subsp. vulgare cDNA clone HS01E20
5-PRIME, mRNA sequence
Length = 583
Score = 119 bits (254), Expect = 2e-026
Identities = 44/56 (78%), Positives = 46/56 (82%)
Frame = -2 / +2
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSD 424
MH NCPVC EYLFDS ISVLHCGHTIHLECL EMR H FSCPVC RSAC+M+D
Sbjct: 413 MHQNCPVCYEYLFDSTMDISVLHCGHTIHLECLNEMRVHHHFSCPVCSRSACDMTD 580
>gb|BQ664207.1|BQ664207 HV02F10u HV Hordeum vulgare subsp. vulgare cDNA clone HV02F10
3-PRIME, mRNA sequence
Length = 497
Score = 73.0 bits (153), Expect(2) = 8e-026
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / -3
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 360 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 247
Score = 63.4 bits (132), Expect(2) = 8e-026
Identities = 21/40 (52%), Positives = 31/40 (77%)
Frame = -2 / -3
Query: 513 TIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQVA 394
TIH +CL EM+ H QF+CP+C +S C+MS W++LD ++A
Sbjct: 495 TIHEKCLKEMKEHCQFACPLCSKSVCDMSKAWERLDAELA 376
>gb|CA010449.1|CA010449 HT09N07u HT Hordeum vulgare subsp. vulgare cDNA clone HT09N07
3-PRIME, mRNA sequence
Length = 488
Score = 73.0 bits (153), Expect(2) = 4e-025
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / -1
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 356 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 243
Score = 61.1 bits (127), Expect(2) = 4e-025
Identities = 20/39 (51%), Positives = 30/39 (76%)
Frame = -2 / -1
Query: 510 IHLECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQVA 394
IH +CL EM+ H QF+CP+C +S C+MS W++LD ++A
Sbjct: 488 IHEKCLKEMKEHCQFACPLCSKSVCDMSKAWERLDAELA 372
>gb|AL505025.1|AL505025 AL505025 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07C11V 5', mRNA sequence
Length = 478
Score = 112 bits (239), Expect = 2e-024
Identities = 37/66 (56%), Positives = 51/66 (77%)
Frame = -2 / +3
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHH+CP+C EYLF+S +SVL CGHTIH +CL EM+ H QF+CP+C +S C+M W++
Sbjct: 261 MHHDCPICFEYLFESRNDVSVLPCGHTIHEKCLKEMKEHCQFACPLCSKSVCDMXKAWER 440
Query: 411 LDQQVA 394
LD ++A
Sbjct: 441 LDAELA 458
>gb|AV928420.1|AV928420 AV928420 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basdg02 3', mRNA sequence
Length = 752
Score = 73.0 bits (153), Expect(2) = 7e-024
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / -1
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 626 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 513
Score = 57.0 bits (118), Expect(2) = 7e-024
Identities = 18/36 (50%), Positives = 28/36 (77%)
Frame = -2 / -1
Query: 501 ECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQVA 394
+CL EM+ H QF+CP+C +S C+MS W++LD ++A
Sbjct: 749 KCLKEMKEHCQFACPLCSKSVCDMSKAWERLDAELA 642
>gb|AV935894.1|AV935894 AV935894 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6o11 3', mRNA sequence
Length = 699
Score = 84.0 bits (177), Expect(2) = 9e-024
Identities = 34/79 (43%), Positives = 46/79 (58%)
Frame = -2 / -2
Query: 468 FSCPVCLRSACNMSDIWQKLDQQVAASPMPAIYQKKMVWILCNDCGVTSNVQFHILAHKC 289
++CP+C +S +M+ + LD +AA +P Y+ + ILCNDC QFH L HKC
Sbjct: 347 YTCPICCKSLGDMAVYFGMLDALLAAEELPEEYRDRCQDILCNDCERKGRSQFHWLYHKC 168
Query: 288 PGCSSYNTRQTRGDPAACS 232
C SYNTR + D A CS
Sbjct: 167 GSCGSYNTRVIKTDTADCS 111
Score = 45.5 bits (93), Expect(2) = 9e-024
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -2 / -2
Query: 582 NCPVCMEYLFDSMKAISVLHCGHTIHLEC 496
NCP+C ++LF S A+ L CGH +H C
Sbjct: 458 NCPICCDFLFTSSAAVRALPCGHFMHSAC 372
>gb|BJ475980.1|BJ475980 BJ475980 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal30o13 3', mRNA sequence
Length = 648
Score = 84.0 bits (177), Expect(2) = 9e-024
Identities = 34/79 (43%), Positives = 46/79 (58%)
Frame = -2 / -2
Query: 468 FSCPVCLRSACNMSDIWQKLDQQVAASPMPAIYQKKMVWILCNDCGVTSNVQFHILAHKC 289
++CP+C +S +M+ + LD +AA +P Y+ + ILCNDC QFH L HKC
Sbjct: 350 YTCPICCKSLGDMAVYFGMLDALLAAEELPEEYRDRCQDILCNDCERKGRSQFHWLYHKC 171
Query: 288 PGCSSYNTRQTRGDPAACS 232
C SYNTR + D A CS
Sbjct: 170 GSCGSYNTRVIKTDTADCS 114
Score = 45.5 bits (93), Expect(2) = 9e-024
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -2 / -2
Query: 582 NCPVCMEYLFDSMKAISVLHCGHTIHLEC 496
NCP+C ++LF S A+ L CGH +H C
Sbjct: 461 NCPICCDFLFTSSAAVRALPCGHFMHSAC 375
>gb|AV920842.1|AV920842 AV920842 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15m20 3', mRNA sequence
Length = 712
Score = 80.8 bits (170), Expect(2) = 2e-022
Identities = 32/79 (40%), Positives = 46/79 (58%)
Frame = -2 / -1
Query: 468 FSCPVCLRSACNMSDIWQKLDQQVAASPMPAIYQKKMVWILCNDCGVTSNVQFHILAHKC 289
++CP+C +S +M+ + LD +AA +P Y+ + ILCNDCG +FH L HKC
Sbjct: 400 YTCPICSKSLGDMTVYFGMLDGLLAAEELPEEYRNRCQDILCNDCGRKGLSRFHWLYHKC 221
Query: 288 PGCSSYNTRQTRGDPAACS 232
C SYNTR + + CS
Sbjct: 220 GACGSYNTRVIKTEAPGCS 164
Score = 44.1 bits (90), Expect(2) = 2e-022
Identities = 14/29 (48%), Positives = 19/29 (65%)
Frame = -2 / -1
Query: 582 NCPVCMEYLFDSMKAISVLHCGHTIHLEC 496
NCP+C ++LF S A+ L CGH +H C
Sbjct: 511 NCPICCDFLFTSSAAVRGLPCGHFMHSAC 425
>gb|CA001227.1|CA001227 HS18N03u HS Hordeum vulgare subsp. vulgare cDNA clone HS18N03
3-PRIME, mRNA sequence
Length = 568
Score = 104 bits (221), Expect = 5e-022
Identities = 37/44 (84%), Positives = 41/44 (93%)
Frame = -2 / -1
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQTRGDPA 241
YQKKMVWILCNDCG TS+V+FH+LA KCPGCSSYNTR+TRG PA
Sbjct: 562 YQKKMVWILCNDCGATSSVRFHVLAQKCPGCSSYNTRETRGGPA 431
>gb|AV911026.1|AV911026 AV911026 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11m03 3', mRNA sequence
Length = 685
Score = 73.0 bits (153), Expect(2) = 6e-021
Identities = 26/38 (68%), Positives = 29/38 (76%)
Frame = -2 / -3
Query: 372 YQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+ KMV ILCNDCG S VQFH++AHKC C SYNTRQ
Sbjct: 578 FDDKMVRILCNDCGAVSEVQFHLIAHKCHSCKSYNTRQ 465
Score = 46.9 bits (96), Expect(2) = 6e-021
Identities = 14/30 (46%), Positives = 23/30 (76%)
Frame = -2 / -3
Query: 483 RAHQQFSCPVCLRSACNMSDIWQKLDQQVA 394
+ H QF+CP+C +S C+MS W++LD ++A
Sbjct: 683 KEHCQFACPLCSKSVCDMSKAWERLDAELA 594
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 365,627,981
Number of Sequences: 312970
Number of extensions: 6808358
Number of successful extensions: 101195
Number of sequences better than 1.0e-020: 35
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6150422805
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)