TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3670899.2.1
(555 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL503069.1|AL503069 AL503069 Hordeum vulgare Barke roots... 265 1e-070
gb|AV934996.1|AV934996 AV934996 K. Sato unpublished cDNA li... 82 3e-033
gb|AV934849.1|AV934849 AV934849 K. Sato unpublished cDNA li... 82 3e-033
gb|BQ768402.1|BQ768402 EBro08_SQ011_I17_R root, 3 week, dro... 122 2e-027
gb|CB877731.1|CB877731 HP05P15T HP Hordeum vulgare subsp. v... 102 2e-021
>gb|AL503069.1|AL503069 AL503069 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09J19u 3', mRNA sequence
Length = 562
Score = 265 bits (573), Expect = 1e-070
Identities = 103/117 (88%), Positives = 110/117 (94%)
Frame = -3 / -1
Query: 487 PSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVAYSFAGVKPWILIRPDWN 308
PSHEE+Q+Y SN+HNQKALAEIYLLSYCDKI MSAWSTFGYVAY FAGVKPWIL+RPDW+
Sbjct: 529 PSHEEKQEYASNEHNQKALAEIYLLSYCDKIAMSAWSTFGYVAYGFAGVKPWILLRPDWD 350
Query: 307 RETSDVACVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKLFDS* 137
+E S VACVRSTSVEPCLHSPPILGCRAK +VDVA VKPYVRHCEDVGFGLKLFDS*
Sbjct: 349 KEVSQVACVRSTSVEPCLHSPPILGCRAKKEVDVARVKPYVRHCEDVGFGLKLFDS* 179
Score = 209 bits (450), Expect(2) = 1e-053
Identities = 93/117 (79%), Positives = 96/117 (82%)
Frame = +3 / +3
Query: 138 *LSNNLRPKPTSSQCRTYGLTAATSTSVLALHPRIGGECRHGSTDVDRTHATSEVSLFQS 317
*LS +LRP PTSSQCRT GLT ATSTS LA P IGGECRHGSTDVDRTHAT + SL QS
Sbjct: 180 *LSKSLRPNPTSSQCRT*GLTRATSTSFLARQPSIGGECRHGSTDVDRTHATCDTSLSQS 359
Query: 318 GRIRIHGFTPAKLYAT*PKVDQALIVILSQ*LSR*ISASAFWLWSLDVYCCCSSWLG 488
GR RIHG TPAK YAT P VDQAL+ ILSQ*LSR*ISASAFWL SLD Y C SSWLG
Sbjct: 360 GRSRIHGLTPAKPYATYPNVDQALMAILSQ*LSR*ISASAFWLCSLDAYSCFSSWLG 530
Score = 156 bits (336), Expect(2) = 4e-040
Identities = 71/93 (76%), Positives = 76/93 (81%)
Frame = +2 / +2
Query: 137 LAIEQLEAEADVLAVPDVRLDSGDVDVRLGPAPEDRRRVQARLHGRGPDARDVGGLPVPV 316
LAIE+LE E DVLAVPDV LD+G V + LGPA E RRRVQARL GRGPDAR + L VPV
Sbjct: 179 LAIEELETEPDVLAVPDVGLDAGHVHLLLGPAAEYRRRVQARLDGRGPDARHLRHLLVPV 358
Query: 317 RPDQDPRLHAGEAVRDVAEGGPGAHRDLVAVAE 415
RP+QDPRL AGEAVRDV E GPGAH DLVAVAE
Sbjct: 359 RPEQDPRLDAGEAVRDVPERGPGAHGDLVAVAE 457
Score = 104 bits (222), Expect = 4e-022
Identities = 50/81 (61%), Positives = 53/81 (65%)
Frame = -2 / -3
Query: 485 QPRGAAAVHVQRPQPEGAGRDLPAQLLRQDHDEXXXXXXXXXXXXXXREAVDPDPAGLEQ 306
+PRG A V VQR QPEGAG DLPAQLLRQD E R+AVDP PAGL Q
Sbjct: 527 EPRGEAGVRVQRAQPEGAGGDLPAQLLRQDRHERLVHVRVRRVRLRRRQAVDPAPAGLGQ 348
Query: 305 GDLRRRVRPVHVRGAVPALAA 243
G + VRPVHVR AVPALAA
Sbjct: 347 GGVAGGVRPVHVRRAVPALAA 285
Score = 27.6 bits (54), Expect(2) = 4e-040
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +1 / +1
Query: 445 CGRWTCTAAAPRG 483
C RWT T A+PRG
Sbjct: 487 CARWTRTPASPRG 525
Score = 21.7 bits (41), Expect(2) = 1e-053
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +2 / +3
Query: 485 GLEHRHDLP 511
G HRHDLP
Sbjct: 528 GXVHRHDLP 554
>gb|AV934996.1|AV934996 AV934996 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2h20 3', mRNA sequence
Length = 593
Score = 81.7 bits (172), Expect(2) = 3e-033
Identities = 30/57 (52%), Positives = 42/57 (73%)
Frame = -3 / -2
Query: 496 VFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVAYSFAGVKPWIL 326
V QPSHE QQ++ H+++AL+E+YLLS CD + + +STFGYVA GV+PWI+
Sbjct: 400 VHQPSHEGQQKWGDTSHDKRALSEMYLLSTCDVLVTTGFSTFGYVAQGLGGVRPWIM 230
Score = 79.4 bits (167), Expect(2) = 3e-033
Identities = 29/46 (63%), Positives = 34/46 (73%)
Frame = -3 / -2
Query: 286 CVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKL 149
CVR+ SVEPC HSP C A+ DVDV V PYVRHCEDV +G+K+
Sbjct: 172 CVRAMSVEPCFHSPSYYDCTARKDVDVGKVLPYVRHCEDVSWGIKI 35
Score = 63.8 bits (133), Expect(2) = 7e-023
Identities = 29/48 (60%), Positives = 33/48 (68%)
Frame = +3 / +3
Query: 153 LRPKPTSSQCRTYGLTAATSTSVLALHPRIGGECRHGSTDVDRTHATS 296
L P+ TSSQCRTYG T TSTS LA+ GE +HGSTD+ RTH S
Sbjct: 39 LIPQLTSSQCRTYGSTFPTSTSFLAVQS**DGEWKHGSTDIARTHGGS 182
Score = 62.5 bits (130), Expect(2) = 7e-023
Identities = 27/56 (48%), Positives = 33/56 (58%)
Frame = +3 / +3
Query: 330 IHGFTPAKLYAT*PKVDQALIVILSQ*LSR*ISASAFWLWSLDVYCCCSSWLGWNT 497
IHG TP AT P V++ ++ SQ LSR IS SA W + + CC SWLGW T
Sbjct: 234 IHGRTPPSPCATYPNVEKPVVTSTSQVLSRYISLSALLSWDVSPHFCCPSWLGWCT 401
>gb|AV934849.1|AV934849 AV934849 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1o03 3', mRNA sequence
Length = 554
Score = 81.7 bits (172), Expect(2) = 3e-033
Identities = 30/57 (52%), Positives = 42/57 (73%)
Frame = -3 / -2
Query: 496 VFQPSHEEQQQYTSNDHNQKALAEIYLLSYCDKITMSAWSTFGYVAYSFAGVKPWIL 326
V QPSHE QQ++ H+++AL+E+YLLS CD + + +STFGYVA GV+PWI+
Sbjct: 400 VHQPSHEGQQKWGDTSHDKRALSEMYLLSTCDVLVTTGFSTFGYVAQGLGGVRPWIM 230
Score = 79.4 bits (167), Expect(2) = 3e-033
Identities = 29/46 (63%), Positives = 34/46 (73%)
Frame = -3 / -2
Query: 286 CVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKL 149
CVR+ SVEPC HSP C A+ DVDV V PYVRHCEDV +G+K+
Sbjct: 172 CVRAMSVEPCFHSPSYYDCTARKDVDVGKVLPYVRHCEDVSWGIKI 35
Score = 63.8 bits (133), Expect(2) = 7e-023
Identities = 29/48 (60%), Positives = 33/48 (68%)
Frame = +3 / +3
Query: 153 LRPKPTSSQCRTYGLTAATSTSVLALHPRIGGECRHGSTDVDRTHATS 296
L P+ TSSQCRTYG T TSTS LA+ GE +HGSTD+ RTH S
Sbjct: 39 LIPQLTSSQCRTYGSTFPTSTSFLAVQS**DGEWKHGSTDIARTHGGS 182
Score = 62.5 bits (130), Expect(2) = 7e-023
Identities = 27/56 (48%), Positives = 33/56 (58%)
Frame = +3 / +3
Query: 330 IHGFTPAKLYAT*PKVDQALIVILSQ*LSR*ISASAFWLWSLDVYCCCSSWLGWNT 497
IHG TP AT P V++ ++ SQ LSR IS SA W + + CC SWLGW T
Sbjct: 234 IHGRTPPSPCATYPNVEKPVVTSTSQVLSRYISLSALLSWDVSPHFCCPSWLGWCT 401
>gb|BQ768402.1|BQ768402 EBro08_SQ011_I17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ011_I17 5', mRNA sequence
Length = 430
Score = 122 bits (261), Expect = 2e-027
Identities = 49/52 (94%), Positives = 50/52 (96%)
Frame = -3 / +3
Query: 292 VACVRSTSVEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKLFDS* 137
VACVRSTSVEPCLHSPPILGCRAK +VDVA VKPYVRHCEDVGFGLKLFDS*
Sbjct: 6 VACVRSTSVEPCLHSPPILGCRAKKEVDVARVKPYVRHCEDVGFGLKLFDS* 161
Score = 100 bits (214), Expect = 5e-021
Identities = 43/52 (82%), Positives = 44/52 (84%)
Frame = +3 / -1
Query: 138 *LSNNLRPKPTSSQCRTYGLTAATSTSVLALHPRIGGECRHGSTDVDRTHAT 293
*LS +LRP PTSSQCRT GLT ATSTS LA P IGGECRHGSTDVDRTHAT
Sbjct: 160 *LSKSLRPNPTSSQCRT*GLTRATSTSFLARQPSIGGECRHGSTDVDRTHAT 5
>gb|CB877731.1|CB877731 HP05P15T HP Hordeum vulgare subsp. vulgare cDNA clone HP05P15
5-PRIME, mRNA sequence
Length = 142
Score = 102 bits (217), Expect = 2e-021
Identities = 40/44 (90%), Positives = 41/44 (93%)
Frame = -3 / +3
Query: 268 VEPCLHSPPILGCRAKTDVDVAAVKPYVRHCEDVGFGLKLFDS* 137
VEPCLHSPPILGCRA +VDVA VKPYVRHCEDVGFGLKLFDS*
Sbjct: 3 VEPCLHSPPILGCRANKEVDVARVKPYVRHCEDVGFGLKLFDS* 134
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 258,657,670
Number of Sequences: 312970
Number of extensions: 4068067
Number of successful extensions: 117961
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5725776986
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)