TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3665525.2.1
(620 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa... 206 1e-052
gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa... 116 2e-050
gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgar... 182 1e-045
>gb|BG414688.2|BG414688 HVSMEk0003F21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0003F21f, mRNA sequence
Length = 625
Score = 206 bits (444), Expect = 1e-052
Identities = 83/113 (73%), Positives = 89/113 (78%)
Frame = -2 / +3
Query: 529 LHAYGPIYXXXXXXXXXXXXLRNNSFRMFSI*DVTIGSWMLAMNVNHENTHALCEPDCTE 350
LHAYGPIY LRNNSFRMF+ DVTIGSWMLAMNVNHENTHALCEP+CT
Sbjct: 3 LHAYGPIYALSADVVASLVALRNNSFRMFNNEDVTIGSWMLAMNVNHENTHALCEPECTA 182
Query: 349 SSVAVWDIPKCSGLCHPEVKMLELHQRKECTGGPTVAAEVSESED**FLGWNT 191
SS+AVWDIPKCSGLCHPEVKMLELH+RKECTGGPT AAE ++* LG T
Sbjct: 183 SSIAVWDIPKCSGLCHPEVKMLELHRRKECTGGPTEAAETETDDE*CLLGLET 341
Score = 158 bits (339), Expect = 3e-038
Identities = 63/94 (67%), Positives = 72/94 (76%)
Frame = -1 / +2
Query: 527 SCIWANLCFIS*RGG*LGCFEEQQFPYV*YLGRYNWIVDACYERQP*EHTCAL*T*LHGV 348
SCIWA LCFIS* GG LGC E+QQFP V GRY+WI+DACYERQP EH C L +HG+
Sbjct: 5 SCIWAYLCFIS*CGGKLGCSEKQQFPNVQQRGRYHWILDACYERQPREHPCPLRARVHGI 184
Query: 347 FSCCLGHSKMLRAMPPRGKNARASSKKRMHRWSN 246
C LGH KM RAMPPRGK+A AS +R+HRWS+
Sbjct: 185 LDCRLGHPKMFRAMPPRGKDAGASPAERVHRWSD 286
Score = 155 bits (333), Expect = 2e-037
Identities = 69/113 (61%), Positives = 74/113 (65%)
Frame = +3 / -1
Query: 192 VFQPRNHQSSDSDTSAATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQ 371
V +P H SS SAA+VGPPVHSF IFTSGWHSPEHFGMSQTA ED+V SGSQ
Sbjct: 340 VSRPSKHHSSSVSVSAASVGPPVHSFRR*SSSIFTSGWHSPEHFGMSQTAIEDAVHSGSQ 161
Query: 372 SACVFSWLTFIASIHDPIVTS*ILNIRXXXXXXXXXXXXXXXXXXXIGPYA*R 530
A VFSWLTFIASI DP+VTS +LNIR IGPYA*R
Sbjct: 160 RAWVFSWLTFIASIQDPMVTSSLLNIRKLLFLRATKLATTSADKA*IGPYA*R 2
Score = 146 bits (314), Expect = 9e-035
Identities = 63/93 (67%), Positives = 71/93 (76%)
Frame = +1 / -2
Query: 250 DHLCILFFDEALAFLPLGGIALSILECPKQQLKTPCSQVHRAHVCSHG*RS*QASTIQL* 429
DH C L EA A LPLGGIAL+IL CP++Q + PC++ R H CS G*RS*QAS IQ *
Sbjct: 282 DHRCTLSAGEAPASLPLGGIALNILGCPRRQSRMPCTRARRGHGCSRG*RS*QASRIQW* 103
Query: 430 RPRY*TYGNCCSSKQPS*PPRQLIKHKLAHMHE 528
RPR *T+GNCC S+QPS PP QLIKHK AHMHE
Sbjct: 102 RPRC*TFGNCCFSEQPSLPPHQLIKHK*AHMHE 4
Score = 138 bits (295), Expect = 4e-032
Identities = 62/93 (66%), Positives = 70/93 (75%)
Frame = +2 / -3
Query: 251 TTCAFFSLMKL*HFYLWVA*P*AFWNVPNSN*RLRAVRFTERMCVLMVDVHSKHPRSNCN 430
TT A F +KL H YLWVA*P* FW+VP+ N RA+ E M VL+VDVHSKHP SN N
Sbjct: 281 TTGALFPPVKLQHLYLWVA*P*TFWDVPDGNRGCRALGLAEGMGVLVVDVHSKHPGSNGN 102
Query: 431 VLDTKHTETVVPQSNQASHHVS**SINWPICMK 529
VL +H+ETVV QSNQA HH+S**SIN PICMK
Sbjct: 101 VLVVEHSETVVSQSNQACHHIS**SINRPICMK 3
>gb|BG416042.1|BG416042 HVSMEk0009I22f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009I22f, mRNA sequence
Length = 1066
Score = 116 bits (248), Expect(2) = 2e-050
Identities = 48/64 (75%), Positives = 49/64 (76%)
Frame = -2 / +2
Query: 619 IGCMKKGPVFTDPKLKWYEPQSFLLGSEYFLHAYGPIYXXXXXXXXXXXXLRNNSFRMFS 440
IGCMKKGPVFTDPKLKW EPQSFLLGSEYFLHAYGPIY LRNNSFRMF+
Sbjct: 26 IGCMKKGPVFTDPKLKWCEPQSFLLGSEYFLHAYGPIYALSADVVASLVALRNNSFRMFN 205
Query: 439 I*DV 428
DV
Sbjct: 206 NEDV 217
Score = 108 bits (230), Expect(3) = 3e-043
Identities = 48/64 (75%), Positives = 54/64 (84%)
Frame = +2 / -1
Query: 428 NVLDTKHTETVVPQSNQASHHVS**SINWPICMKKVF*SE*EGLGLIPFQLRISKNRPLL 607
NVL +H+ETVV QSNQA HH+S**SIN PICMK+VF*S+*EGL L PFQ IS+NRP L
Sbjct: 217 NVLVVEHSETVVSQSNQACHHIS**SINRPICMKEVF*SK*EGLWLTPFQFGISENRPFL 38
Query: 608 HASN 619
HASN
Sbjct: 37 HASN 26
Score = 107 bits (228), Expect(2) = 7e-033
Identities = 49/64 (76%), Positives = 52/64 (81%)
Frame = +1 / -3
Query: 427 *RPRY*TYGNCCSSKQPS*PPRQLIKHKLAHMHEESILIRVRRIGAHTIST*DQ*KQAPS 606
*RPR *T+GNCC S+QPS PP QLIKHK AHMHE SILI+V R AHTIS DQ*KQA S
Sbjct: 218 *RPRC*TFGNCCFSEQPSLPPHQLIKHK*AHMHEGSILIQVGRTVAHTISIWDQ*KQALS 39
Query: 607 SCIQ 618
SCIQ
Sbjct: 38 SCIQ 27
Score = 103 bits (220), Expect(2) = 9e-035
Identities = 46/64 (71%), Positives = 50/64 (78%)
Frame = -1 / +1
Query: 620 HWMHEEGACFY*S*VEMV*APILLTRIRILSSCIWANLCFIS*RGG*LGCFEEQQFPYV* 441
HWMHEE ACF+*S +EMV*A +L T IRIL SCIWA LCFIS* GG LGC E+QQFP V
Sbjct: 25 HWMHEERACFH*SQIEMV*ATVLPTWIRILPSCIWAYLCFIS*CGGKLGCSEKQQFPNVQ 204
Query: 440 YLGR 429
GR
Sbjct: 205 QRGR 216
Score = 102 bits (218), Expect(2) = 2e-050
Identities = 37/47 (78%), Positives = 41/47 (87%)
Frame = -2 / +3
Query: 430 VTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVK 290
+TIGSWMLAMNVNHEN HALCEP+C S+A WDIPKCSGLCHP+ K
Sbjct: 216 LTIGSWMLAMNVNHENPHALCEPECNGFSIADWDIPKCSGLCHPKGK 356
Score = 95.0 bits (201), Expect(2) = 6e-029
Identities = 40/65 (61%), Positives = 44/65 (67%)
Frame = +3 / -2
Query: 426 VTS*ILNIRXXXXXXXXXXXXXXXXXXXIGPYA*RKYSDPSKKDWGSYHFNLGSVKTGPF 605
+TS +LNIR IGPYA*RKYSDPS+KD GS+HFNLGSVKTGPF
Sbjct: 219 LTSSLLNIRKLLFLRATKLATTSADKA*IGPYA*RKYSDPSRKDCGSHHFNLGSVKTGPF 40
Query: 606 FMHPM 620
FMHPM
Sbjct: 39 FMHPM 25
Score = 81.7 bits (172), Expect(3) = 3e-043
Identities = 32/43 (74%), Positives = 36/43 (83%)
Frame = +3 / -1
Query: 300 GWHSPEHFGMSQTATEDSVQSGSQSACVFSWLTFIASIHDPIV 428
GWHSPEHFGMSQ+A E+ + SGSQ A FSWLTFIASI DP+V
Sbjct: 346 GWHSPEHFGMSQSAIENPLHSGSQRAWGFSWLTFIASIQDPMV 218
Score = 71.6 bits (150), Expect(2) = 2e-021
Identities = 31/41 (75%), Positives = 35/41 (85%)
Frame = -3 / +3
Query: 618 LDA*RRGLFLLILS*NGMSPNPSYSDQNTFFMHMGQFMLYQ 496
LDA*R+GLF LI +*NG+S +PSY DQNT FMHMG FMLYQ
Sbjct: 27 LDA*RKGLFSLIPN*NGVSHSPSYLDQNTSFMHMGLFMLYQ 149
Score = 62.9 bits (131), Expect(2) = 9e-035
Identities = 24/43 (55%), Positives = 29/43 (67%)
Frame = -1 / +2
Query: 425 NWIVDACYERQP*EHTCAL*T*LHGVFSCCLGHSKMLRAMPPR 297
+WI+DACYERQP E C L + + C LGH KM RAMPP+
Sbjct: 221 HWILDACYERQPREPPCPLRARVQRILDCRLGHPKMFRAMPPQ 349
Score = 52.8 bits (109), Expect(2) = 7e-033
Identities = 25/45 (55%), Positives = 29/45 (64%)
Frame = +2 / -3
Query: 290 FYLWVA*P*AFWNVPNSN*RLRAVRFTERMCVLMVDVHSKHPRSN 424
F VA*P* FW+VP N A+ + M VL+VDVHSKHP SN
Sbjct: 356 FTFGVA*P*TFWDVPVGNRESVALGLAKGMGVLVVDVHSKHPGSN 222
Score = 51.9 bits (107), Expect(2) = 6e-029
Identities = 23/41 (56%), Positives = 29/41 (70%)
Frame = +1 / -2
Query: 301 GGIALSILECPKQQLKTPCSQVHRAHVCSHG*RS*QASTIQ 423
GGIAL+IL CP +Q + C++ + H S G*RS*QAS IQ
Sbjct: 345 GGIALNILGCPSRQSRIRCTRARKGHGGSRG*RS*QASRIQ 223
Score = 50.1 bits (103), Expect(2) = 2e-021
Identities = 25/45 (55%), Positives = 29/45 (64%)
Frame = -3 / +1
Query: 423 LDRGCLL*TSTMRTHMRSVNLTARSLQLLFGTFQNAQGYATQR*K 289
LD GCLL*TST RT M + +A +L GT QN QGYAT + K
Sbjct: 223 LDPGCLL*TSTTRTPMPFASPSATDSRLPTGTSQNVQGYATPKVK 357
Score = 23.1 bits (44), Expect(3) = 3e-043
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +2 / -2
Query: 251 TTCAFFSLMKL*HFYLW 301
T C F KL H YLW
Sbjct: 396 TGCTFSRR*KLQHLYLW 346
>gb|CK122677.1|CK122677 BES1824105a18 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010A185 5-PRIME, mRNA sequence
Length = 627
Score = 182 bits (393), Expect = 1e-045
Identities = 72/90 (80%), Positives = 78/90 (86%)
Frame = -2 / +1
Query: 460 NSFRMFSI*DVTIGSWMLAMNVNHENTHALCEPDCTESSVAVWDIPKCSGLCHPEVKMLE 281
NSFRMF+ DVTIGSWMLAMNVNHENTHALCEP+CT SS+AVWDIPKCSGLCHPEVKMLE
Sbjct: 1 NSFRMFNNEDVTIGSWMLAMNVNHENTHALCEPECTASSIAVWDIPKCSGLCHPEVKMLE 180
Query: 280 LHQRKECTGGPTVAAEVSESED**FLGWNT 191
LH+RKECTGGPT AAE ++* LG T
Sbjct: 181 LHRRKECTGGPTEAAETETDDE*CLLGLET 270
Score = 146 bits (313), Expect = 1e-034
Identities = 62/87 (71%), Positives = 67/87 (77%)
Frame = +3 / -2
Query: 192 VFQPRNHQSSDSDTSAATVGPPVHSFLXXXXXIFTSGWHSPEHFGMSQTATEDSVQSGSQ 371
V +P H SS SAA+VGPPVHSF IFTSGWHSPEHFGMSQTA ED+V SGSQ
Sbjct: 269 VSRPSKHHSSSVSVSAASVGPPVHSFRR*SSSIFTSGWHSPEHFGMSQTAIEDAVHSGSQ 90
Query: 372 SACVFSWLTFIASIHDPIVTS*ILNIR 452
A VFSWLTFIASI DP+VTS +LNIR
Sbjct: 89 RAWVFSWLTFIASIQDPMVTSSLLNIR 9
Score = 121 bits (258), Expect = 5e-027
Identities = 47/71 (66%), Positives = 55/71 (77%)
Frame = -1 / +3
Query: 458 QFPYV*YLGRYNWIVDACYERQP*EHTCAL*T*LHGVFSCCLGHSKMLRAMPPRGKNARA 279
QFP V GRY+WI+DACYERQP*EH C L T +HG+ C LGH KM RAMPPRGK+A A
Sbjct: 3 QFPNVQQRGRYHWILDACYERQP*EHPCPLRTRVHGILDCRLGHPKMFRAMPPRGKDAGA 182
Query: 278 SSKKRMHRWSN 246
S +R+HRWS+
Sbjct: 183 SPAERVHRWSD 215
Score = 108 bits (231), Expect = 2e-023
Identities = 47/70 (67%), Positives = 54/70 (77%)
Frame = +1 / -3
Query: 250 DHLCILFFDEALAFLPLGGIALSILECPKQQLKTPCSQVHRAHVCSHG*RS*QASTIQL* 429
DH C L EA A LPLGGIAL+IL CP++Q + PC++V R H CSHG*RS*QAS IQ *
Sbjct: 211 DHRCTLSAGEAPASLPLGGIALNILGCPRRQSRMPCTRVRRGHGCSHG*RS*QASRIQW* 32
Query: 430 RPRY*TYGNC 459
RPR *T+GNC
Sbjct: 31 RPRC*TFGNC 2
Score = 100 bits (213), Expect = 7e-021
Identities = 45/70 (64%), Positives = 51/70 (72%)
Frame = +2 / -1
Query: 251 TTCAFFSLMKL*HFYLWVA*P*AFWNVPNSN*RLRAVRFTERMCVLMVDVHSKHPRSNCN 430
TT A F +KL H YLWVA*P* FW+VP+ N RA+ F E M VLMVDVHSKHP SN N
Sbjct: 210 TTGALFPPVKLQHLYLWVA*P*TFWDVPDGNRGCRALGFAEGMGVLMVDVHSKHPGSNGN 31
Query: 431 VLDTKHTETV 460
VL +H+ETV
Sbjct: 30 VLVVEHSETV 1
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 307,208,777
Number of Sequences: 312970
Number of extensions: 4781622
Number of successful extensions: 52091
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6574589895
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)