TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3642920.2.1
(570 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgar... 100 5e-043
gb|BU973760.1|BU973760 HB25O06r BC Hordeum vulgare subsp. v... 100 7e-043
gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA li... 96 3e-042
gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA li... 95 1e-041
gb|BF266558.3|BF266558 HV_CEa0015H07f Hordeum vulgare seedl... 106 5e-040
gb|BU988833.1|BU988833 HF18N17r HF Hordeum vulgare subsp. v... 155 1e-037
gb|BG416331.2|BG416331 HVSMEk0011G12f Hordeum vulgare testa... 68 1e-029
gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. v... 90 2e-029
gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. v... 105 8e-029
gb|AV833832.1|AV833832 AV833832 K. Sato unpublished cDNA li... 100 1e-027
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 105 4e-025
gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA li... 106 5e-025
gb|BF264428.1|BF264428 HV_CEa0009G10f Hordeum vulgare seedl... 110 8e-024
gb|CB883229.1|CB883229 HQ01J14w HQ Hordeum vulgare subsp. v... 105 1e-022
gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, dro... 90 5e-022
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 101 3e-021
gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. v... 100 5e-021
>gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P119 5-PRIME, mRNA sequence
Length = 787
Score = 100 bits (213), Expect(2) = 5e-043
Identities = 41/92 (44%), Positives = 57/92 (61%)
Frame = +3 / +1
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
H+ + SDNI+A SVV+ ST +S PE+IVFH++TD+ Y M +WFALN AIVE++
Sbjct: 148 HYCVFSDNIIAVSVVINSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALNDYRGAIVEIQ 327
Query: 186 GVHQFDWLTQENVPVLESIETQRAARDRYHGS 281
V F WL VPVL+ ++ + GS
Sbjct: 328 KVEDFTWLNASYVPVLKQLQDAATQNFYFSGS 423
Score = 93.6 bits (198), Expect(2) = 5e-043
Identities = 35/57 (61%), Positives = 48/57 (84%)
Frame = +3 / +1
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETC 515
+P Y ++LNH+R Y+PE++P L+KV+FLDDD+VVQ DLS L+ I+L+G V GAVETC
Sbjct: 454 NPKYLSMLNHLRFYIPEIYPELHKVLFLDDDIVVQKDLSDLFTINLNGNVMGAVETC 624
>gb|BU973760.1|BU973760 HB25O06r BC Hordeum vulgare subsp. vulgare cDNA clone HB25O06
5-PRIME, mRNA sequence
Length = 563
Score = 99.6 bits (211), Expect(2) = 7e-043
Identities = 43/102 (42%), Positives = 59/102 (57%)
Frame = +3 / +1
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
H+ L SDN+LAASVVV STI ++ +PE+ VFH++TDK + AM+ WF LN A + V+
Sbjct: 82 HYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLNPPGKATIHVE 261
Query: 186 GVHQFDWLTQENVPVLESIETQRAARDRYHGSRRPITSANDS 311
V +F WL PVL +E+ + R SA S
Sbjct: 262 NVDEFKWLNSSYCPVLRQLESVAMKEYYFKADRPATLSAGSS 387
Score = 94.1 bits (199), Expect(2) = 7e-043
Identities = 32/60 (53%), Positives = 50/60 (83%)
Frame = +3 / +1
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
++ L+ +P Y ++LNH+R Y+P+++P L+K++FLDDD+VVQ DL+ LWD+DL+G VNGA
Sbjct: 382 SSNLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGA 561
>gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 5', mRNA sequence
Length = 596
Score = 96.4 bits (204), Expect(2) = 3e-042
Identities = 40/87 (45%), Positives = 54/87 (62%)
Frame = +3 / +3
Query: 21 SDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQF 200
SDNI+A SVV+ ST +S PE+IVFH++TD+ Y M +WFALN AIVE++ V F
Sbjct: 18 SDNIIAVSVVINSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALNDYRGAIVEIQKVEDF 197
Query: 201 DWLTQENVPVLESIETQRAARDRYHGS 281
WL VPVL+ ++ + GS
Sbjct: 198 TWLNASYVPVLKQLQDAATQNFYFSGS 278
Score = 95.0 bits (201), Expect(2) = 3e-042
Identities = 36/57 (63%), Positives = 48/57 (84%)
Frame = +3 / +3
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETC 515
+P Y ++LNH+R Y+PE++P L+KVVFLDDD+VVQ DLS L+ I+L+G V GAVETC
Sbjct: 309 NPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETC 479
>gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g14 5', mRNA sequence
Length = 500
Score = 95.0 bits (201), Expect(2) = 1e-041
Identities = 36/57 (63%), Positives = 48/57 (84%)
Frame = +3 / +2
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETC 515
+P Y ++LNH+R Y+PE++P L+KVVFLDDD+VVQ DLS L+ I+L+G V GAVETC
Sbjct: 308 NPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVETC 478
Score = 94.5 bits (200), Expect(2) = 1e-041
Identities = 40/87 (45%), Positives = 53/87 (60%)
Frame = +3 / +2
Query: 21 SDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQF 200
SDNI+A SVV ST +S PE+IVFH++TD+ Y M +WFALN AIVE++ V F
Sbjct: 17 SDNIIAVSVVXNSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALNDYRGAIVEIQKVEDF 196
Query: 201 DWLTQENVPVLESIETQRAARDRYHGS 281
WL VPVL+ ++ + GS
Sbjct: 197 TWLNASYVPVLKQLQDAATQNFYFSGS 277
>gb|BF266558.3|BF266558 HV_CEa0015H07f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0015H07f, mRNA sequence
Length = 767
Score = 106 bits (227), Expect(2) = 5e-040
Identities = 39/66 (59%), Positives = 53/66 (80%)
Frame = +3 / +3
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
A+ ++ +P Y ++LNH+R Y+PE++P L K++FLDDDVVVQ DL+ LW ID+ GKVNGA
Sbjct: 390 ASNMKFRNPKYLSMLNHLRFYLPEMYPKLQKILFLDDDVVVQRDLTGLWKIDMDGKVNGA 569
Query: 504 VETCRG 521
VETC G
Sbjct: 570 VETCFG 587
Score = 77.1 bits (162), Expect(2) = 5e-040
Identities = 35/81 (43%), Positives = 47/81 (58%)
Frame = +3 / +3
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
H+ + SDN+LAASVVVRS + +S P + VFHV+TD+ AM L L A EVK
Sbjct: 93 HYAIFSDNVLAASVVVRSCVANSQDPSKHVFHVVTDRMNLGAMQVIIRLMDLQGAHYEVK 272
Query: 186 GVHQFDWLTQENVPVLESIET 248
+ +L VPVL +E+
Sbjct: 273 AYEDYKFLNSSYVPVLRQLES 335
>gb|BU988833.1|BU988833 HF18N17r HF Hordeum vulgare subsp. vulgare cDNA clone HF18N17
5-PRIME, mRNA sequence
Length = 610
Score = 155 bits (334), Expect = 1e-037
Identities = 63/70 (90%), Positives = 68/70 (97%)
Frame = +3 / +1
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
HFVLASDNILAASVVVRST+RS+LKPERIVFHVITDKKTYPAMHSWFALN L PAI+EVK
Sbjct: 400 HFVLASDNILAASVVVRSTVRSALKPERIVFHVITDKKTYPAMHSWFALNPLYPAIIEVK 579
Query: 186 GVHQFDWLTQ 215
GVHQF+WLT+
Sbjct: 580 GVHQFEWLTK 609
Score = 121 bits (259), Expect = 3e-027
Identities = 55/69 (79%), Positives = 58/69 (84%)
Frame = -3 / -3
Query: 211 VNQSN**TPFTSTIAGLREFKANHECMAG*VFLSVITWKTILSGFSDDLIVDLTTTEAAR 32
VN SN TPFTS +AG R F ANHECMAG*VFLSV+T KTILSGF+ DL VDLTTTEAAR
Sbjct: 605 VNHSNWWTPFTSIMAG*RGFNANHECMAG*VFLSVMT*KTILSGFNADLTVDLTTTEAAR 426
Query: 31 MLSDANTKW 5
MLSDA TKW
Sbjct: 425 MLSDAKTKW 399
Score = 102 bits (218), Expect = 1e-021
Identities = 42/69 (60%), Positives = 50/69 (72%)
Frame = +2 / +3
Query: 5 PFCVSIR*HPCSLSCGQINN*IITEA*EDCFPCYY**KDLSCHAFMVCFKFSQSCYC*GE 184
P C +R HPCS CGQIN+*I EA*ED C+Y* +DLSCHAFMVC + S SC+ *GE
Sbjct: 399 PLCFGVRQHPCSFGCGQINS*ICIEA*EDSLSCHY*QEDLSCHAFMVCIESSLSCHY*GE 578
Query: 185 RCSSI*LVN 211
C I*+V+
Sbjct: 579 GCPPI*MVD 605
>gb|BG416331.2|BG416331 HVSMEk0011G12f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0011G12f, mRNA sequence
Length = 1003
Score = 68.0 bits (142), Expect(3) = 1e-029
Identities = 33/94 (35%), Positives = 52/94 (55%)
Frame = +3 / +1
Query: 183 KGVHQFDWLTQENVPVLESIETQRAARDRYHGSRRPITSANDSPRVFAAKLQAGSPTYTN 362
K ++ FD LT+ENVPVL++ + R ++ +HG+ I+ + PR FA+KLQA SP +
Sbjct: 625 KVLNHFDCLTRENVPVLKAFKNHRGVKNHFHGNSGTISHGSAHPRAFASKLQAQSPKSIS 804
Query: 363 VLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSP 464
+ H F SL+K ++VV + P
Sbjct: 805 AV*HCAHLFAIPFSSLHKRGLS*KELVVTRNFLP 906
Score = 62.0 bits (129), Expect(3) = 1e-029
Identities = 21/28 (75%), Positives = 27/28 (96%)
Frame = +3 / +2
Query: 81 PERIVFHVITDKKTYPAMHSWFALNSLS 164
PE++VFHV+T+KKT+P MHSWFALNS+S
Sbjct: 521 PEKVVFHVLTNKKTFPGMHSWFALNSVS 604
Score = 36.8 bits (74), Expect(3) = 1e-029
Identities = 14/21 (66%), Positives = 18/21 (85%)
Frame = +3 / +1
Query: 6 HFVLASDNILAASVVVRSTIR 68
H++LASDNILAAS+ ST+R
Sbjct: 445 HYILASDNILAASICGNSTVR 507
>gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. vulgare cDNA clone HV09J15
5-PRIME, mRNA sequence
Length = 586
Score = 89.5 bits (189), Expect(2) = 2e-029
Identities = 34/57 (59%), Positives = 46/57 (80%)
Frame = +3 / +2
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETC 515
+P + ++LNH+R Y+P++ P+L KVVFLDDDVVVQ DL+ L+ I+L G V GAVETC
Sbjct: 272 NPKFVSLLNHLRFYIPQILPNLEKVVFLDDDVVVQKDLTQLFSIELHGNVIGAVETC 442
Score = 60.2 bits (125), Expect(2) = 2e-029
Identities = 21/53 (39%), Positives = 34/53 (64%)
Frame = +3 / +2
Query: 48 VVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQFDW 206
VV ST+ ++ P+++VFHV+TD+ + AM +WF +N VEV + +F W
Sbjct: 11 VVNSTVSNANHPQQLVFHVVTDRINFGAMSTWFLINDFKGCTVEVHCIDEFSW 169
>gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. vulgare cDNA clone HA17H16
5-PRIME, mRNA sequence
Length = 554
Score = 105 bits (223), Expect(2) = 8e-029
Identities = 36/64 (56%), Positives = 54/64 (84%)
Frame = +3 / +3
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
++ L+ +P Y ++LNH+R Y+P+++P LNK++FLDDD+VVQ DL+ LW++DL+G VNGA
Sbjct: 183 SSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQKDLTGLWEVDLNGNVNGA 362
Query: 504 VETC 515
VETC
Sbjct: 363 VETC 374
Score = 41.4 bits (84), Expect(2) = 8e-029
Identities = 18/57 (31%), Positives = 25/57 (43%)
Frame = +3 / +3
Query: 141 WFALNSLSPAIVEVKGVHQFDWLTQENVPVLESIETQRAARDRYHGSRRPITSANDS 311
WF LN A + V+ V F WL PVL+ +E+ + R+ SA S
Sbjct: 18 WFLLNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRQKTLSAGSS 188
>gb|AV833832.1|AV833832 AV833832 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags20k03, mRNA sequence
Length = 526
Score = 100 bits (214), Expect(2) = 1e-027
Identities = 35/64 (54%), Positives = 53/64 (82%)
Frame = +3 / +2
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
++ L+ +P Y ++LNH+R Y+P+++P L+K++FLDDD+VVQ DL+ LWD+DL+G VNGA
Sbjct: 206 SSNLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGA 385
Query: 504 VETC 515
V TC
Sbjct: 386 VFTC 397
Score = 41.4 bits (84), Expect(2) = 1e-027
Identities = 18/61 (29%), Positives = 27/61 (44%)
Frame = +3 / +2
Query: 129 AMHSWFALNSLSPAIVEVKGVHQFDWLTQENVPVLESIETQRAARDRYHGSRRPITSAND 308
+++ WF LN A + V+ V +F WL PVL +E+ + R SA
Sbjct: 29 SLNMWFLLNPPGKATIHVENVDEFKWLNSSYCPVLRQLESVAMKEYYFKADRPATLSAGS 208
Query: 309 S 311
S
Sbjct: 209 S 211
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 105 bits (223), Expect(2) = 4e-025
Identities = 36/64 (56%), Positives = 54/64 (84%)
Frame = +3 / +3
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
++ L+ +P Y ++LNH+R Y+P+++P LNK++FLDDD+VVQ DL+ LW++DL+G VNGA
Sbjct: 141 SSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQKDLTGLWEVDLNGNVNGA 320
Query: 504 VETC 515
VETC
Sbjct: 321 VETC 332
Score = 29.0 bits (57), Expect(2) = 4e-025
Identities = 13/44 (29%), Positives = 19/44 (43%)
Frame = +3 / +3
Query: 180 VKGVHQFDWLTQENVPVLESIETQRAARDRYHGSRRPITSANDS 311
V+ V F WL PVL+ +E+ + R+ SA S
Sbjct: 15 VENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRQKTLSAGSS 146
>gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 5', mRNA sequence
Length = 606
Score = 106 bits (227), Expect(2) = 5e-025
Identities = 39/66 (59%), Positives = 53/66 (80%)
Frame = +3 / +1
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
A+ ++ +P Y ++LNH+R Y+PE++P L K++FLDDDVVVQ DL+ LW ID+ GKVNGA
Sbjct: 184 ASNMKFRNPKYLSMLNHLRFYLPEMYPKLQKILFLDDDVVVQRDLTGLWKIDMDGKVNGA 363
Query: 504 VETCRG 521
VETC G
Sbjct: 364 VETCFG 381
Score = 26.7 bits (52), Expect(2) = 5e-025
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +3 / +1
Query: 129 AMHSWFALNSLSPAIVEVKGVHQFDWLTQENVPVLESIET 248
AM L L A EVK + +L VPVL +E+
Sbjct: 10 AMQVIIRLMDLQGAHYEVKAYEDYKFLNSSYVPVLRQLES 129
>gb|BF264428.1|BF264428 HV_CEa0009G10f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0009G10f, mRNA sequence
Length = 839
Score = 110 bits (234), Expect = 8e-024
Identities = 41/67 (61%), Positives = 53/67 (79%)
Frame = +3 / +3
Query: 6 HFVLASDNILAASVVVRSTIRSSLKPERIVFHVITDKKTYPAMHSWFALNSLSPAIVEVK 185
H + +DN+LA +V V S R+S P R+VFHV+TDKK+Y MHSWFAL+ +SPA+VEVK
Sbjct: 144 HVCIVTDNVLAGAVAVSSAGRASAGPSRLVFHVVTDKKSYVPMHSWFALHPVSPAVVEVK 323
Query: 186 GVHQFDW 206
G+HQFDW
Sbjct: 324 GLHQFDW 344
>gb|CB883229.1|CB883229 HQ01J14w HQ Hordeum vulgare subsp. vulgare cDNA clone HQ01J14
3-PRIME, mRNA sequence
Length = 147
Score = 105 bits (225), Expect = 1e-022
Identities = 41/48 (85%), Positives = 44/48 (91%)
Frame = +3 / +3
Query: 90 IVFHVITDKKTYPAMHSWFALNSLSPAIVEVKGVHQFDWLTQENVPVL 233
IVFHVITDKKTYPAMHSWFALN L PAI+EVKGVH F+WLT+EN PVL
Sbjct: 3 IVFHVITDKKTYPAMHSWFALNPLYPAIIEVKGVHHFEWLTKENGPVL 146
>gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ002_D17 5', mRNA sequence
Length = 562
Score = 89.5 bits (189), Expect(2) = 5e-022
Identities = 34/57 (59%), Positives = 46/57 (80%)
Frame = +3 / +2
Query: 345 SPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGAVETC 515
+P + ++LNH+R Y+P++ P+L KVVFLDDDVVVQ DL+ L+ I+L G V GAVETC
Sbjct: 203 NPKFVSLLNHLRFYIPQILPNLEKVVFLDDDVVVQKDLTQLFSIELHGNVIGAVETC 373
Score = 35.4 bits (71), Expect(2) = 5e-022
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +3 / +2
Query: 108 TDKKTYPAMHSWFALNSLSPAIVEVKGVHQFDW 206
TD+ + AM +WF +N VEV + +F W
Sbjct: 2 TDRINFGAMSTWFLINDFKGCTVEVHCIDEFSW 100
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 101 bits (216), Expect = 3e-021
Identities = 37/69 (53%), Positives = 55/69 (79%)
Frame = +3 / +2
Query: 309 SPRVFAAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSG 488
S R ++ L+ +P Y ++LNH+R Y+P+++P L+K++FLDDD+VVQ DL+ LWD+DL+G
Sbjct: 2 SARGGSSNLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNG 181
Query: 489 KVNGAVETC 515
VNGAV TC
Sbjct: 182 MVNGAVFTC 208
>gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. vulgare cDNA clone HB08B19
5-PRIME, mRNA sequence
Length = 521
Score = 100 bits (214), Expect = 5e-021
Identities = 35/64 (54%), Positives = 53/64 (82%)
Frame = +3 / +2
Query: 324 AAKLQAGSPTYTNVLNHIRIYMPELFPSLNKVVFLDDDVVVQHDLSPLWDIDLSGKVNGA 503
++ L+ +P Y ++LNH+R Y+P+++P L+K++FLDDD+VVQ DL+ LWD+DL+G VNGA
Sbjct: 110 SSNLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGA 289
Query: 504 VETC 515
V TC
Sbjct: 290 VFTC 301
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 254,129,634
Number of Sequences: 312970
Number of extensions: 3658592
Number of successful extensions: 40253
Number of sequences better than 1.0e-020: 21
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 5853505842
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)