TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3345642.2.1
(600 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green... 347 3e-095
gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa... 318 4e-087
gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA li... 259 1e-068
gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA li... 259 1e-068
gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm librar... 249 8e-066
gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgar... 249 8e-066
gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA li... 248 3e-065
gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. v... 248 3e-065
gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. v... 243 5e-064
gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA li... 187 6e-060
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 226 1e-058
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 221 4e-057
gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-a... 216 6e-056
gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-a... 198 2e-050
gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day ger... 195 1e-049
gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA li... 193 7e-049
gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-a... 182 1e-045
gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day ger... 172 1e-042
gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA li... 168 3e-041
gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA li... 164 5e-040
gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, dro... 95 4e-038
gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no... 153 7e-037
gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA li... 88 8e-037
gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA li... 153 1e-036
gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day ger... 151 2e-036
gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA li... 147 6e-035
gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. v... 142 2e-033
gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA li... 141 4e-033
gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA li... 140 5e-033
gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA li... 140 5e-033
gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA li... 140 5e-033
gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA li... 132 2e-030
gb|AJ432742.1|AJ432742 AJ432742 S00002 Hordeum vulgare subs... 121 3e-027
gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. v... 120 6e-027
gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA li... 119 1e-026
gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. v... 92 6e-026
gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. v... 116 8e-026
gb|BU986456.1|BU986456 HF11O19r HF Hordeum vulgare subsp. v... 114 4e-025
gb|AV834161.1|AV834161 AV834161 K. Sato unpublished cDNA li... 92 3e-024
gb|BQ460226.1|BQ460226 HA06N23r HA Hordeum vulgare subsp. v... 105 2e-022
>gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0025O15f, mRNA sequence
Length = 674
Score = 347 bits (752), Expect = 3e-095
Identities = 137/159 (86%), Positives = 144/159 (90%)
Frame = +1 / +1
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
GNVIGAVETCLESFHRYHK+ FS P ISSKIDP+TCGWAFGMNIFDLIAWRKANATSLY
Sbjct: 109 GNVIGAVETCLESFHRYHKWGGFSQPIISSKIDPYTCGWAFGMNIFDLIAWRKANATSLY 288
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHY 366
HYWQEQN+D LLWRTGTLPAGLLTFYGLMEPLDRRWH+LGLGYDVDIDDRLIESAAVVHY
Sbjct: 289 HYWQEQNADQLLWRTGTLPAGLLTFYGLMEPLDRRWHLLGLGYDVDIDDRLIESAAVVHY 468
Query: 367 NGNMKPWLKLAIRRYKYIWERYVNISHPYVRECMLH*KV 483
NGNMKPWLKLAI YK IW VN SHP++RE + H* V
Sbjct: 469 NGNMKPWLKLAIHPYKSIWGADVNFSHPHIREGVFH*AV 585
Score = 267 bits (578), Expect = 3e-071
Identities = 123/160 (76%), Positives = 132/160 (82%)
Frame = -2 / -1
Query: 485 QTF*CNIHSLTYGCEIFTYRSHIYL*RRIASFSQGFMFPL*CTTAALSIRRSSMSTSYPR 306
QT * N SL GCE T HI L +A+FSQGFMFPL*CTTAALSI RSSMSTSYPR
Sbjct: 587 QTA**NTPSLICGCEKLTSAPHIDLYG*MANFSQGFMFPL*CTTAALSINRSSMSTSYPR 408
Query: 305 PKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFIPK 126
P CHLRSRGSIRP*NV+RPAG VPVLQSN S FCSCQ** +DVAFAFLQAI+S +FIPK
Sbjct: 407 PNRCHLRSRGSIRP*NVRRPAGSVPVLQSN*SAFCSCQ***SDVAFAFLQAIRSNIFIPK 228
Query: 125 AHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
AHPQV GSI ELI+GCEK +L*YR*NDSK VSTAP+TLP
Sbjct: 227 AHPQVYGSIFELIIGCEKPPHL*YR*NDSKHVSTAPITLP 108
Score = 225 bits (486), Expect = 2e-058
Identities = 99/158 (62%), Positives = 120/158 (75%)
Frame = -1 / -3
Query: 483 DFLMQHTLSNIRV*DIHIPFPYILVTANSQLQPGFHVSIIVHNSSTLNQTIIYVYIIPET 304
D L++HTLSN+ V*+I I PY LV N QLQPGFHV I+VHNSSTLNQ +IYVYIIP+T
Sbjct: 585 DCLVKHTLSNMWV*EIDIRSPYRLVRVNGQLQPGFHVPIVVHNSSTLNQPVIYVYIIPKT 406
Query: 303 QDMPSAV*GLHQAIKCQKACRKSSCSPEQ*VRILLLPIMIQ*CGVCLPPSYQVKNVHSKS 124
Q +PS V*GLHQAI+CQ+AC K + P+Q + IL LPIMI+ C +C PPS VK +HSKS
Sbjct: 405 Q*VPSPV*GLHQAIECQEACWKCARPPKQLISILFLPIMIKRCRICFPPSN*VKYIHSKS 226
Query: 123 PSTSMWINLGADSRMRKI*ILVIPMKRL*TSFHCSNDI 10
P TS+W+NL AD +R+ VIPMK L*T HC N+I
Sbjct: 225 PPTSIWVNL*ADYWLRETPPFVIPMK*L*TRLHCPNNI 112
Score = 220 bits (475), Expect = 5e-057
Identities = 94/158 (59%), Positives = 116/158 (73%)
Frame = +3 / +3
Query: 12 CHWSSGNLFRXXXXXXXXXKFFASYYQLQD*STYLWMGFWNEHF*LDSLEEGKRHIIVSL 191
C+W SG++FR F A+ QL+D* YLW+GFWNE+ * + LEE K I +SL
Sbjct: 114 CYWGSGDVFRVISSVSQMGGFLATNNQLKD*PIYLWVGFWNEYI*PNCLEESKCDIALSL 293
Query: 192 LARAKF*PIALENRNSSCRPFDILWPDGAPRPQMACLGSRV*CRHR*SSD*ECCCCAL*W 371
LA + * IALE+ ++S RP DILWPDGAPRP+MA +GS V*CRHR* D*ECCCCAL W
Sbjct: 294 LAGTEC*SIALEDGHTSSRPPDILWPDGAPRPEMAPIGSWV*CRHR*PVD*ECCCCALQW 473
Query: 372 KHETLAEAGYSPLQVYMGTVCEYLTPVC*RVYVALESL 485
+HETLAE G+SP+QVYMG+ C++LTP *R V+L SL
Sbjct: 474 EHETLAEVGHSPVQVYMGSGCQFLTPTY*RGCVSLGSL 587
Score = 197 bits (424), Expect = 6e-050
Identities = 99/158 (62%), Positives = 112/158 (70%)
Frame = +2 / +2
Query: 11 MSLEQWKLV*SRFIGITSI*IFRILLSAPRLIHILVDGLLE*TFLT**LGGRQTPHHCII 190
M L QW+ V*S FIGIT+ + R SA RL HILV GLLE* +LT* LGG+Q H II
Sbjct: 113 MLLGQWRRV*SHFIGITNGGVSRNQ*SAQRLTHILVGGLLE*IYLT*LLGGKQMRHRFII 292
Query: 191 IGKSKILTYCSGEQELFLQAF*HFMA*WSP*TADGMSWVSGMM*T*MIV*LRVLLLCTIM 370
IG++++L C G + F QA *H MA*WSP*T DG WV GMM*T*M *LRVLLLCT M
Sbjct: 293 IGRNRMLINCFGGRAHFQQAS*HSMA*WSP*TGDGTYWVLGMM*T*MTG*LRVLLLCTTM 472
Query: 371 ET*NPG*SWLFAVTSIYGNGM*ISHTRMLESVCCIRKS 484
T*NPG*SW F TS+YG M ISHT +LE VC R+S
Sbjct: 473 GT*NPG*SWPFTRTSLYGERMSISHTHILERVCFTRQS 586
Score = 179 bits (386), Expect = 1e-044
Identities = 91/162 (56%), Positives = 101/162 (62%)
Frame = -3 / -2
Query: 496 TEICRLSNATYTL*HTGVRYSHTVPIYTCNGE*PASARVSCFHYSAXXXXXXXXXHLCLH 317
T RL + T+ L*+ GVR H +PI TC GE P SARVSC H SA HLCLH
Sbjct: 598 TRDARLPSETHPL*YVGVRN*HPLPI*TCTGEWPTSARVSCSHCSAQQQHSQSTGHLCLH 419
Query: 316 HTRDPRHAICGLGAPSGHKMSKGLQEEFLFSRAIGQNFALANNDTMMWRLPSSKLSSQKC 137
HT+DP AI GLGAPSGH+MS GL E S+AI Q+ ANND M L SSK Q
Sbjct: 418 HTQDPIGAISGLGAPSGHRMSGGLLEVCPSSKAIDQHSVPANNDKAMSHLLSSKQLGQIY 239
Query: 136 SFQKPIHKYVDQSWS***DAKNLDTCDTDETTLNKFPLLQ*H 11
SFQKP HKY+ QS S* A+N CDTDE TLN PL Q*H
Sbjct: 238 SFQKPTHKYMGQSLS*LLVARNPPICDTDEMTLNTSPLPQ*H 113
>gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016D06f, mRNA sequence
Length = 490
Score = 318 bits (689), Expect(2) = 4e-087
Identities = 122/135 (90%), Positives = 128/135 (94%)
Frame = +1 / +3
Query: 82 PTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNSDLLLWRTGTLPAGLLTF 261
P ISSKIDP+TCGWAFGMNIFDLIAWRKANATSLYHYWQEQN+D LLWRTGTLPAGLLTF
Sbjct: 6 PIISSKIDPYTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNADQLLWRTGTLPAGLLTF 185
Query: 262 YGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIRRYKYIWERYVNI 441
YGLMEPLDRRWH+LGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAI RYK IWER+VN
Sbjct: 186 YGLMEPLDRRWHLLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIHRYKSIWERHVNF 365
Query: 442 SHPYVRECMLH*KVC 486
SHP++RECM H* VC
Sbjct: 366 SHPHIRECMFH*AVC 410
Score = 245 bits (529), Expect(2) = 3e-065
Identities = 110/137 (80%), Positives = 117/137 (85%)
Frame = -2 / -1
Query: 488 LQTF*CNIHSLTYGCEIFTYRSHIYL*RRIASFSQGFMFPL*CTTAALSIRRSSMSTSYP 309
LQT * NIHSL GCE T RSHI L R +A+FSQGFMFPL*CTTAALSI RSSMSTSYP
Sbjct: 412 LQTA**NIHSLICGCEKLTCRSHIDLYR*MANFSQGFMFPL*CTTAALSINRSSMSTSYP 233
Query: 308 RPKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFIP 129
RP CHLRSRGSIRP*NV+RPAG VPVLQSN S FCSCQ** +DVAFAFLQAI+S +FIP
Sbjct: 232 RPNRCHLRSRGSIRP*NVRRPAGSVPVLQSN*SAFCSCQ***SDVAFAFLQAIRSNIFIP 53
Query: 128 KAHPQVCGSILELIVGC 78
KAHPQV GSI ELI+GC
Sbjct: 52 KAHPQVYGSIFELIIGC 2
Score = 211 bits (455), Expect = 3e-054
Identities = 90/137 (65%), Positives = 108/137 (78%)
Frame = +3 / +2
Query: 78 ASYYQLQD*STYLWMGFWNEHF*LDSLEEGKRHIIVSLLARAKF*PIALENRNSSCRPFD 257
A+ QL+D* YLW+GFWNE+ * + LEE K I +SLLA + * IALE+ ++S RP D
Sbjct: 2 ATNNQLKD*PIYLWVGFWNEYI*PNCLEESKCDIALSLLAGTEC*SIALEDGHTSSRPPD 181
Query: 258 ILWPDGAPRPQMACLGSRV*CRHR*SSD*ECCCCAL*WKHETLAEAGYSPLQVYMGTVCE 437
ILWPDGAPRP+MA +GS V*CRHR* D*ECCCCAL W+HETLAE G+SP+QVYMG C+
Sbjct: 182 ILWPDGAPRPEMAPIGSWV*CRHR*PVD*ECCCCALQWEHETLAEVGHSPVQVYMGAACQ 361
Query: 438 YLTPVC*RVYVALESLQ 488
+LTP *RVYV+L SLQ
Sbjct: 362 FLTPTY*RVYVSLGSLQ 412
Score = 207 bits (446), Expect = 5e-053
Identities = 87/133 (65%), Positives = 106/133 (79%)
Frame = -1 / -3
Query: 486 ADFLMQHTLSNIRV*DIHIPFPYILVTANSQLQPGFHVSIIVHNSSTLNQTIIYVYIIPE 307
AD L++HTLSN+ V*+I +P PY LV N QLQPGFHV I+VHNSSTLNQ +IYVYIIP+
Sbjct: 410 ADCLVKHTLSNMWV*EIDMPLPYRLVPVNGQLQPGFHVPIVVHNSSTLNQPVIYVYIIPK 231
Query: 306 TQDMPSAV*GLHQAIKCQKACRKSSCSPEQ*VRILLLPIMIQ*CGVCLPPSYQVKNVHSK 127
TQ +PS V*GLHQAI+CQ+AC K + P+Q + IL LPIMI+ C +C PPS VK +HSK
Sbjct: 230 TQ*VPSPV*GLHQAIECQEACWKCARPPKQLISILFLPIMIKRCRICFPPSN*VKYIHSK 51
Query: 126 SPSTSMWINLGAD 88
SP TS+W+NL AD
Sbjct: 50 SPPTSIWVNL*AD 12
Score = 184 bits (397), Expect = 3e-046
Identities = 89/133 (66%), Positives = 100/133 (75%)
Frame = +2 / +1
Query: 89 SAPRLIHILVDGLLE*TFLT**LGGRQTPHHCIIIGKSKILTYCSGEQELFLQAF*HFMA 268
SA RL HILV GLLE* +LT* LGG+Q H IIIG++++L C G + F QA *H MA
Sbjct: 13 SAQRLTHILVGGLLE*IYLT*LLGGKQMRHRFIIIGRNRMLINCFGGRAHFQQAS*HSMA 192
Query: 269 *WSP*TADGMSWVSGMM*T*MIV*LRVLLLCTIMET*NPG*SWLFAVTSIYGNGM*ISHT 448
*WSP*T DG WV GMM*T*M *LRVLLLCT M T*NPG*SW F TS+YG+GM ISHT
Sbjct: 193 *WSP*TGDGTYWVLGMM*T*MTG*LRVLLLCTTMGT*NPG*SWPFTGTSLYGSGMSISHT 372
Query: 449 RMLESVCCIRKSA 487
+LESVC R+SA
Sbjct: 373 HILESVCFTRQSA 411
Score = 160 bits (344), Expect = 6e-039
Identities = 78/133 (58%), Positives = 85/133 (63%)
Frame = -3 / -2
Query: 487 CRLSNATYTL*HTGVRYSHTVPIYTCNGE*PASARVSCFHYSAXXXXXXXXXHLCLHHTR 308
CRL + TYTL*+ GVR H PI TC GE P SARVSC H SA HLCLHHT+
Sbjct: 411 CRLPSETYTL*YVGVRN*HAAPI*TCTGEWPTSARVSCSHCSAQQQHSQSTGHLCLHHTQ 232
Query: 307 DPRHAICGLGAPSGHKMSKGLQEEFLFSRAIGQNFALANNDTMMWRLPSSKLSSQKCSFQ 128
DP AI GLGAPSGH+MS GL E S+AI Q+ ANND M L SSK Q SFQ
Sbjct: 231 DPIGAISGLGAPSGHRMSGGLLEVCPSSKAIDQHSVPANNDKAMSHLLSSKQLGQIYSFQ 52
Query: 127 KPIHKYVDQSWS* 89
KP HKY+ QS S*
Sbjct: 51 KPTHKYMGQSLS* 13
Score = 23.5 bits (45), Expect(2) = 3e-065
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = -1 / -1
Query: 552 KQPHVKKLKHAASVPCKNI 496
K +KKLK ASVP K I
Sbjct: 484 KATPLKKLKFLASVPWKTI 428
Score = 23.1 bits (44), Expect(2) = 4e-087
Identities = 9/14 (64%), Positives = 9/14 (64%)
Frame = +2 / +3
Query: 497 MFLHGTEAACFSFF 538
M HGTEA SFF
Sbjct: 429 MVFHGTEARNLSFF 470
>gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 5', mRNA sequence
Length = 630
Score = 259 bits (559), Expect = 1e-068
Identities = 96/154 (62%), Positives = 111/154 (72%)
Frame = +1 / +2
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ YLNFSHP IS DP CGWAFGMN+FDL W+K N T +
Sbjct: 20 KGMVNGAVETCKESFHRFDTYLNFSHPKISENFDPRACGWAFGMNMFDLKEWKKRNITGI 199
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVH 363
YHYWQ N D LW+ GTLP GL+TFY L PLD WHVLGLGYD +D IE+AAVVH
Sbjct: 200 YHYWQNLNEDRKLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAAVVH 379
Query: 364 YNGNMKPWLKLAIRRYKYIWERYVNISHPYVREC 465
YNGN KPWL LAI +YK W +YV++ ++R C
Sbjct: 380 YNGNYKPWLDLAISKYKAYWSKYVDVDSSHIRHC 481
Score = 133 bits (284), Expect = 1e-030
Identities = 71/143 (49%), Positives = 82/143 (57%)
Frame = -2 / -1
Query: 434 TYRSHIYL*RRIASFSQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NV 255
TY L +A SQG FPL* TTAA SI S + S PRPKTC + S G ++ *NV
Sbjct: 450 TYLDQ*ALYLEMARSSQGL*FPL**TTAAFSISAMSTAGS*PRPKTCQV*SNGCVKL*NV 271
Query: 254 KRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFIPKAHPQVCGSILELIVGCE 75
P G VP + RS F CQ* Y V F F + +S MFIPKA PQ GS I+GCE
Sbjct: 270 ISPGGNVPSFHNLRSSFKFCQ*WYIPVMFRFFHSFRSNMFIPKAQPQARGSKFSEILGCE 91
Query: 74 KFRYL*YR*NDSKQVSTAPMTLP 6
K R + R S QVSTAP T+P
Sbjct: 90 KLR*VSKRWKLSLQVSTAPFTIP 22
>gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 3', mRNA sequence
Length = 565
Score = 259 bits (559), Expect = 1e-068
Identities = 96/154 (62%), Positives = 111/154 (72%)
Frame = +1 / -1
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ YLNFSHP IS DP CGWAFGMN+FDL W+K N T +
Sbjct: 550 KGMVNGAVETCKESFHRFDTYLNFSHPKISENFDPRACGWAFGMNMFDLKEWKKRNITGI 371
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVH 363
YHYWQ N D LW+ GTLP GL+TFY L PLD WHVLGLGYD +D IE+AAVVH
Sbjct: 370 YHYWQNLNEDRKLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGYDPAVDIAEIENAAVVH 191
Query: 364 YNGNMKPWLKLAIRRYKYIWERYVNISHPYVREC 465
YNGN KPWL LAI +YK W +YV++ ++R C
Sbjct: 190 YNGNYKPWLDLAISKYKAYWSKYVDVDSSHIRHC 89
Score = 133 bits (284), Expect = 1e-030
Identities = 71/143 (49%), Positives = 82/143 (57%)
Frame = -2 / +3
Query: 434 TYRSHIYL*RRIASFSQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NV 255
TY L +A SQG FPL* TTAA SI S + S PRPKTC + S G ++ *NV
Sbjct: 120 TYLDQ*ALYLEMARSSQGL*FPL**TTAAFSISAMSTAGS*PRPKTCQV*SNGCVKL*NV 299
Query: 254 KRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFIPKAHPQVCGSILELIVGCE 75
P G VP + RS F CQ* Y V F F + +S MFIPKA PQ GS I+GCE
Sbjct: 300 ISPGGNVPSFHNLRSSFKFCQ*WYIPVMFRFFHSFRSNMFIPKAQPQARGSKFSEILGCE 479
Query: 74 KFRYL*YR*NDSKQVSTAPMTLP 6
K R + R S QVSTAP T+P
Sbjct: 480 KLR*VSKRWKLSLQVSTAPFTIP 548
>gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B7 5' similar to 68 kDa
protein, mRNA sequence
Length = 616
Score = 249 bits (539), Expect = 8e-066
Identities = 92/155 (59%), Positives = 113/155 (72%)
Frame = +1 / +1
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
G V GAV TC ESFHR+ KYLNFS+P I+ DP+ CGWA+GMNIFDL W+ + T +Y
Sbjct: 13 GMVNGAVFTCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGIY 192
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHY 366
H WQ N D +LW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ID I++AAV HY
Sbjct: 193 HKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHY 372
Query: 367 NGNMKPWLKLAIRRYKYIWERYVNISHPYVRECML 471
NGNMKPWL+LA+ +Y+ W RY+ HPY+R C L
Sbjct: 373 NGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 477
Score = 83.5 bits (176), Expect(2) = 1e-027
Identities = 40/69 (57%), Positives = 42/69 (60%)
Frame = -2 / -3
Query: 401 IASFSQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NVKRPAGRVPVLQ 222
IAS S GFMFPL* TAALSI SM YP P+TCH SRG + *NV RP G VP Q
Sbjct: 407 IASSSHGFMFPL**ATAALSISERSMLGLYPSPRTCHDLSRGCVSL*NVMRPGGSVPSFQ 228
Query: 221 SNRSEFCSC 195
S F C
Sbjct: 227 RTLSSFMFC 201
Score = 58.8 bits (122), Expect(2) = 1e-027
Identities = 27/53 (50%), Positives = 32/53 (60%)
Frame = -2 / -3
Query: 164 FLQAIKSKMFIPKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
F +SKMFIP A PQ GS + G EK RYL R S QV+TAP+T+P
Sbjct: 170 FFHCFRSKMFIP*AQPQALGSKFRAMCGFEKLRYLSKRWKLSPQVNTAPLTIP 12
>gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010D065 5-PRIME, mRNA sequence
Length = 786
Score = 249 bits (539), Expect = 8e-066
Identities = 92/155 (59%), Positives = 113/155 (72%)
Frame = +1 / +1
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
G V GAV TC ESFHR+ KYLNFS+P I+ DP+ CGWA+GMNIFDL W+ + T +Y
Sbjct: 52 GMVNGAVFTCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGIY 231
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHY 366
H WQ N D +LW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ID I++AAV HY
Sbjct: 232 HKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHY 411
Query: 367 NGNMKPWLKLAIRRYKYIWERYVNISHPYVRECML 471
NGNMKPWL+LA+ +Y+ W RY+ HPY+R C L
Sbjct: 412 NGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 516
Score = 83.5 bits (176), Expect(2) = 1e-027
Identities = 40/69 (57%), Positives = 42/69 (60%)
Frame = -2 / -2
Query: 401 IASFSQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NVKRPAGRVPVLQ 222
IAS S GFMFPL* TAALSI SM YP P+TCH SRG + *NV RP G VP Q
Sbjct: 446 IASSSHGFMFPL**ATAALSISERSMLGLYPSPRTCHDLSRGCVSL*NVMRPGGSVPSFQ 267
Query: 221 SNRSEFCSC 195
S F C
Sbjct: 266 RTLSSFMFC 240
Score = 58.8 bits (122), Expect(2) = 1e-027
Identities = 27/53 (50%), Positives = 32/53 (60%)
Frame = -2 / -2
Query: 164 FLQAIKSKMFIPKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
F +SKMFIP A PQ GS + G EK RYL R S QV+TAP+T+P
Sbjct: 209 FFHCFRSKMFIP*AQPQALGSKFRAMCGFEKLRYLSKRWKLSPQVNTAPLTIP 51
>gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 3', mRNA sequence
Length = 643
Score = 248 bits (535), Expect = 3e-065
Identities = 87/157 (55%), Positives = 116/157 (73%)
Frame = +1 / -3
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ +YLNFS P I+ DP CGWAFGMN+FDL WR+ N T L
Sbjct: 629 KGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITEL 450
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVH 363
YH+WQ+ N D LLW+ GTLP GL+TF+ PLDR WHVLGLGY+ + R +E AAV+H
Sbjct: 449 YHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIH 270
Query: 364 YNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECMLH 474
YNGN+KPWL++ + +++ W +Y++ P++REC ++
Sbjct: 269 YNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNIN 159
Score = 98.7 bits (209), Expect(2) = 6e-023
Identities = 49/102 (48%), Positives = 58/102 (56%)
Frame = -2 / +1
Query: 311 PRPKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFI 132
PRP+TCH RSRG++ NV RP GRVP S+ S CQ + V F L + +S FI
Sbjct: 322 PRPRTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFI 501
Query: 131 PKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
PKA P GS I G EKF+ R S QVSTAP+T P
Sbjct: 502 PKAQPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFP 627
Score = 28.1 bits (55), Expect(2) = 6e-023
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -2 / +1
Query: 389 SQGFMFPL*CTTAALS 342
SQGF PL*C TAA S
Sbjct: 244 SQGFRLPL*CMTAARS 291
>gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. vulgare cDNA clone HX12J15
5-PRIME, mRNA sequence
Length = 653
Score = 248 bits (535), Expect = 3e-065
Identities = 87/157 (55%), Positives = 116/157 (73%)
Frame = +1 / -3
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ +YLNFS P I+ DP CGWAFGMN+FDL WR+ N T L
Sbjct: 642 KGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITEL 463
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVH 363
YH+WQ+ N D LLW+ GTLP GL+TF+ PLDR WHVLGLGY+ + R +E AAV+H
Sbjct: 462 YHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIH 283
Query: 364 YNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECMLH 474
YNGN+KPWL++ + +++ W +Y++ P++REC ++
Sbjct: 282 YNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNIN 172
Score = 98.7 bits (209), Expect(2) = 6e-023
Identities = 49/102 (48%), Positives = 58/102 (56%)
Frame = -2 / +2
Query: 311 PRPKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFI 132
PRP+TCH RSRG++ NV RP GRVP S+ S CQ + V F L + +S FI
Sbjct: 335 PRPRTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFI 514
Query: 131 PKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
PKA P GS I G EKF+ R S QVSTAP+T P
Sbjct: 515 PKAQPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFP 640
Score = 28.1 bits (55), Expect(2) = 6e-023
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -2 / +2
Query: 389 SQGFMFPL*CTTAALS 342
SQGF PL*C TAA S
Sbjct: 257 SQGFRLPL*CMTAARS 304
>gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. vulgare cDNA clone HX14D07
5-PRIME, mRNA sequence
Length = 529
Score = 243 bits (526), Expect = 5e-064
Identities = 85/153 (55%), Positives = 113/153 (73%)
Frame = +1 / +2
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
G V GAVETC SFHRY +Y+NFSHP I +K +P+ CGWA+GMN FDL +WR+ +T Y
Sbjct: 71 GKVNGAVETCFGSFHRYWQYMNFSHPLIKAKFNPNACGWAYGMNFFDLNSWRREKSTEQY 250
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHY 366
HYWQ QN + LLW+ GTLP GL+TFY +PLD+ WHVLGLGY+ I I +AAVVH+
Sbjct: 251 HYWQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHF 430
Query: 367 NGNMKPWLKLAIRRYKYIWERYVNISHPYVREC 465
NGNMKPWL + + +++ +W +YV+ ++R+C
Sbjct: 431 NGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 529
Score = 143 bits (307), Expect = 8e-034
Identities = 69/128 (53%), Positives = 77/128 (60%)
Frame = -2 / -2
Query: 389 SQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRS 210
SQGF+FPL CTTAA I M YPRP+TC SRG + *NV P G VP S+R
Sbjct: 453 SQGFIFPLKCTTAAFLISSMLMLGLYPRPRTCQDLSRGFVVE*NVINPGGNVPNFHSSRF 274
Query: 209 EFCSCQ**YNDVAFAFLQAIKSKMFIPKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQV 30
F CQ* Y V F+ LQ ++SK F+P A P G LI GCEKF Y YR*ND K V
Sbjct: 273 SF*VCQ*WYCSVDFSLLQELRSKKFMP*AQPHALGLNFALIRGCEKFIYCQYR*NDPKHV 94
Query: 29 STAPMTLP 6
STAP T P
Sbjct: 93 STAPFTFP 70
>gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 5', mRNA sequence
Length = 612
Score = 187 bits (403), Expect(2) = 6e-060
Identities = 70/116 (60%), Positives = 84/116 (72%)
Frame = +1 / +2
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ +YLNFS P I+ DP CGWAFGMN+FDL WR+ N T L
Sbjct: 95 KGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITEL 274
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESA 351
YH+WQ+ N D LLW+ GTLP GL+TF+ PLDR WHVLGLGY+ + R +E A
Sbjct: 275 YHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERA 442
Score = 98.7 bits (209), Expect(2) = 4e-022
Identities = 49/102 (48%), Positives = 58/102 (56%)
Frame = -2 / -1
Query: 311 PRPKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFI 132
PRP+TCH RSRG++ NV RP GRVP S+ S CQ + V F L + +S FI
Sbjct: 402 PRPRTCHDRSRGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFI 223
Query: 131 PKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
PKA P GS I G EKF+ R S QVSTAP+T P
Sbjct: 222 PKAQPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFP 97
Score = 63.4 bits (132), Expect(2) = 6e-060
Identities = 17/41 (41%), Positives = 32/41 (78%)
Frame = +1 / +3
Query: 352 AVVHYNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECMLH 474
AV+HYNGN+KPWL++ + +++ W +Y++ P++REC ++
Sbjct: 444 AVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNIN 566
Score = 25.4 bits (49), Expect(2) = 4e-022
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = -2 / -3
Query: 389 SQGFMFPL*CTTA 351
SQGF PL*C TA
Sbjct: 481 SQGFRLPL*CMTA 443
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 226 bits (487), Expect = 1e-058
Identities = 84/139 (60%), Positives = 103/139 (74%)
Frame = +1 / +2
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
G V GAV TC ESFHR+ KYLNFS+P I+ DP+ CGWA+GMNIFDL W+ + T +Y
Sbjct: 179 GMVNGAVFTCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGIY 358
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHY 366
H WQ N D +LW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ID I++AAV HY
Sbjct: 359 HKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHY 538
Query: 367 NGNMKPWLKLAIRRYKYIW 423
NGNMKPWL+LA+ +Y+ W
Sbjct: 539 NGNMKPWLELAMTKYRPYW 595
Score = 83.5 bits (176), Expect(2) = 1e-027
Identities = 40/69 (57%), Positives = 42/69 (60%)
Frame = -2 / -2
Query: 401 IASFSQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NVKRPAGRVPVLQ 222
IAS S GFMFPL* TAALSI SM YP P+TCH SRG + *NV RP G VP Q
Sbjct: 573 IASSSHGFMFPL**ATAALSISERSMLGLYPSPRTCHDLSRGCVSL*NVMRPGGSVPSFQ 394
Query: 221 SNRSEFCSC 195
S F C
Sbjct: 393 RTLSSFMFC 367
Score = 58.8 bits (122), Expect(2) = 1e-027
Identities = 27/53 (50%), Positives = 32/53 (60%)
Frame = -2 / -2
Query: 164 FLQAIKSKMFIPKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
F +SKMFIP A PQ GS + G EK RYL R S QV+TAP+T+P
Sbjct: 336 FFHCFRSKMFIP*AQPQALGSKFRAMCGFEKLRYLSKRWKLSPQVNTAPLTIP 178
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 221 bits (476), Expect = 4e-057
Identities = 80/127 (62%), Positives = 98/127 (77%)
Frame = +1 / +3
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
GNV GAVETC ESFHR+ KYLNFS+P IS DP+ CGWA+GMN+FDL W+K + T +Y
Sbjct: 303 GNVNGAVETCGESFHRFDKYLNFSNPNISQNFDPNACGWAYGMNMFDLEEWKKKDITGIY 482
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHY 366
H WQ N + LLW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ++ I++AAV+HY
Sbjct: 483 HKWQNMNENRLLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPTVEHAEIDTAAVIHY 662
Query: 367 NGNMKPW 387
NGNMKPW
Sbjct: 663 NGNMKPW 683
>gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006H13f, mRNA sequence
Length = 1018
Score = 216 bits (467), Expect = 6e-056
Identities = 79/137 (57%), Positives = 100/137 (72%)
Frame = +1 / +1
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ +YLNFS P I+ DP CGWAFGMN+FDL WR+ N T L
Sbjct: 262 KGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITEL 441
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVH 363
YH+WQ+ N D LLW+ GTLP GL+TF+ PLDR WHVLGLGY+ + R +E AAV+H
Sbjct: 442 YHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIH 621
Query: 364 YNGNMKPWLKLAIRRYK 414
Y G++KPWL+ + ++
Sbjct: 622 YXGHLKPWLEXGLPSFE 672
Score = 95.9 bits (203), Expect(2) = 7e-024
Identities = 48/102 (47%), Positives = 57/102 (55%)
Frame = -2 / -3
Query: 311 PRPKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRSEFCSCQ**YNDVAFAFLQAIKSKMFI 132
PRP+TCH RS G++ NV RP GRVP S+ S CQ + V F L + +S FI
Sbjct: 569 PRPRTCHDRSXGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFI 390
Query: 131 PKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
PKA P GS I G EKF+ R S QVSTAP+T P
Sbjct: 389 PKAQPHASGSKFFAITGLEKFK*RPKRWKLSPQVSTAPLTFP 264
Score = 34.1 bits (68), Expect(2) = 7e-024
Identities = 11/39 (28%), Positives = 24/39 (61%)
Frame = -1 / -2
Query: 408 TANSQLQPGFHVSIIVHNSSTLNQTIIYVYIIPETQDMP 292
T + L+PG V ++++ TL + Y++++ T++MP
Sbjct: 666 TGEAXLKPGLQVPXVMYDCGTLQVSTAYMWVVT*TENMP 550
>gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018F09f, mRNA sequence
Length = 856
Score = 198 bits (427), Expect = 2e-050
Identities = 70/119 (58%), Positives = 87/119 (73%)
Frame = +1 / +1
Query: 115 CGWAFGMNIFDLIAWRKANATSLYHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRW 294
CGWA+GMNIFDL W+ + T +YH WQ N D +LW+ GTLP GL+TFY L PLD+ W
Sbjct: 4 CGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSW 183
Query: 295 HVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECML 471
HVLGLGY+ ID I++AAV HYNGNMKPWL+LA+ +Y+ W RY+ HPY+R C L
Sbjct: 184 HVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 360
>gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ001_M07 5', mRNA sequence
Length = 512
Score = 195 bits (421), Expect = 1e-049
Identities = 68/103 (66%), Positives = 83/103 (80%)
Frame = +1 / +3
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
GNV+GAVETC+E+FHR+HKYLN SHP I + DP CGWAFGMN+ DL+ WR N T +Y
Sbjct: 180 GNVMGAVETCMETFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIY 359
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGY 315
HYWQE+N+D LW+ G+LP GLL FYGL+E L+ +WHVLGLGY
Sbjct: 360 HYWQERNADHTLWKLGSLPPGLLAFYGLVEALNPKWHVLGLGY 488
>gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 3', mRNA sequence
Length = 625
Score = 193 bits (416), Expect = 7e-049
Identities = 65/124 (52%), Positives = 91/124 (73%)
Frame = +1 / -3
Query: 94 SKIDPHTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNSDLLLWRTGTLPAGLLTFYGLM 273
+K +P+ CGWA+GMN FDL +WR+ +T YHYWQ QN + LLW+ GTLP GL+TFY
Sbjct: 620 AKFNPNACGWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLGTLPPGLITFYSTT 441
Query: 274 EPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIRRYKYIWERYVNISHPY 453
+PLD+ WHVLGLGY+ I I +AAVVH+NGNMKPWL + + +++ +W +YV+ +
Sbjct: 440 KPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSF 261
Query: 454 VREC 465
+R+C
Sbjct: 260 IRQC 249
Score = 100 bits (213), Expect = 7e-021
Identities = 48/95 (50%), Positives = 56/95 (58%)
Frame = -2 / +1
Query: 389 SQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NVKRPAGRVPVLQSNRS 210
SQGF+FPL CTTAA I M YPRP+TC SRG + *NV P G VP S+R
Sbjct: 325 SQGFIFPLKCTTAAFLISSMLMLGLYPRPRTCQDLSRGFVVE*NVINPGGNVPNFHSSRF 504
Query: 209 EFCSCQ**YNDVAFAFLQAIKSKMFIPKAHPQVCG 105
F CQ* Y V F+ LQ ++SK F+P A P G
Sbjct: 505 SF*VCQ*WYCSVDFSLLQELRSKKFMP*AQPHALG 609
>gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012C17f, mRNA sequence
Length = 785
Score = 182 bits (392), Expect = 1e-045
Identities = 67/105 (63%), Positives = 79/105 (75%)
Frame = +1 / +1
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ +YLNFS P I+ DP CGWAFGMN+FDL WR+ N T L
Sbjct: 262 KGKVNGAVETCGESFHRFGRYLNFSSPVIAKNFDPDACGWAFGMNVFDLAEWRRQNITEL 441
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYD 318
YH+WQ+ N D LLW+ GTLP GL+TF+ PLDR WHVLGLGY+
Sbjct: 442 YHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYN 576
>gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_D08 5', mRNA sequence
Length = 464
Score = 172 bits (371), Expect = 1e-042
Identities = 60/93 (64%), Positives = 74/93 (79%)
Frame = +1 / +3
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
GNV+GAVETC+E+FHR+HKYLN SHP I + DP CGWAFGMN+ DL+ WR N T +Y
Sbjct: 180 GNVMGAVETCMETFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIY 359
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLD 285
HYWQE+N+D LW+ G+LP GLL FYGL+E L+
Sbjct: 360 HYWQERNADHTLWKLGSLPPGLLAFYGLVEALN 458
>gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd23f10, mRNA
sequence
Length = 643
Score = 168 bits (361), Expect = 3e-041
Identities = 59/105 (56%), Positives = 75/105 (71%)
Frame = +1 / -2
Query: 157 WRKANATSLYHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDR 336
W+ + T +YH WQ N D +LW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ID
Sbjct: 636 WKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRS 457
Query: 337 LIESAAVVHYNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECML 471
I++AAV HYNGNMKPWL+LA+ +Y+ W RY+ HPY+R C L
Sbjct: 456 EIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 322
>gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 3', mRNA sequence
Length = 634
Score = 164 bits (352), Expect = 5e-040
Identities = 59/104 (56%), Positives = 74/104 (71%)
Frame = +1 / -1
Query: 160 RKANATSLYHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRL 339
R + T +YH WQ N D +LW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ID
Sbjct: 631 RTKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSE 452
Query: 340 IESAAVVHYNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECML 471
I++AAV HYNGNMKPWL+LA+ +Y+ W RY+ HPY+R C L
Sbjct: 451 IDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 320
>gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ002_D17 5', mRNA sequence
Length = 562
Score = 95.0 bits (201), Expect(2) = 4e-038
Identities = 35/37 (94%), Positives = 36/37 (97%)
Frame = +1 / +3
Query: 112 TCGWAFGMNIFDLIAWRKANATSLYHYWQEQNSDLLL 222
TCGWAFGMNIFDLIAWRKANATSLYHYWQEQN+D LL
Sbjct: 450 TCGWAFGMNIFDLIAWRKANATSLYHYWQEQNADQLL 560
Score = 82.6 bits (174), Expect(2) = 4e-038
Identities = 32/35 (91%), Positives = 33/35 (94%)
Frame = +1 / +2
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPH 111
GNVIGAVETCLESFHRYHKYLNFS P ISSKIDP+
Sbjct: 344 GNVIGAVETCLESFHRYHKYLNFSQPIISSKIDPY 448
Score = 71.6 bits (150), Expect(2) = 2e-027
Identities = 31/38 (81%), Positives = 34/38 (89%)
Frame = -2 / -1
Query: 224 QSNRSEFCSCQ**YNDVAFAFLQAIKSKMFIPKAHPQV 111
QSN S FCSCQ** +DVAFAFLQAI+S +FIPKAHPQV
Sbjct: 562 QSN*SAFCSCQ***SDVAFAFLQAIRSNIFIPKAHPQV 449
Score = 70.3 bits (147), Expect(2) = 2e-027
Identities = 29/34 (85%), Positives = 31/34 (91%)
Frame = -2 / -2
Query: 107 GSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
GSI ELI+GCEK RYL*YR*NDSK VSTAP+TLP
Sbjct: 444 GSIFELIIGCEKLRYL*YR*NDSKHVSTAPITLP 343
>gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_G13 5', mRNA sequence
Length = 523
Score = 153 bits (329), Expect = 7e-037
Identities = 55/93 (59%), Positives = 68/93 (73%)
Frame = +1 / +2
Query: 193 WQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNG 372
WQ N D +LW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ID I++AAV HYNG
Sbjct: 2 WQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNG 181
Query: 373 NMKPWLKLAIRRYKYIWERYVNISHPYVRECML 471
NMKPWL+LA+ +Y+ W RY+ HPY+R C L
Sbjct: 182 NMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 280
>gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags20p21, mRNA sequence
Length = 494
Score = 87.7 bits (185), Expect(2) = 8e-037
Identities = 28/52 (53%), Positives = 35/52 (67%)
Frame = +1 / +3
Query: 52 RYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLYHYWQEQN 207
R+ Y NFSHP I +DP C WA+GMN+FDL AWRK N YH+W ++N
Sbjct: 33 RFRTYFNFSHPVIDRSLDPDECAWAYGMNVFDLEAWRKTNIRDTYHFWLKEN 188
Score = 85.8 bits (181), Expect(2) = 8e-037
Identities = 32/64 (50%), Positives = 41/64 (64%)
Frame = +1 / +3
Query: 214 LLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLK 393
L LW+ GTLP L+ F G + +D WH+LGLGY D ++ AAVVHYNG KPWL
Sbjct: 201 LTLWKFGTLPPALIAFRGHVHGIDPSWHMLGLGYQESTDIESVKKAAVVHYNGQCKPWLD 380
Query: 394 LAIR 405
+A +
Sbjct: 381 IAFK 392
>gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 5', mRNA sequence
Length = 606
Score = 153 bits (328), Expect = 1e-036
Identities = 54/86 (62%), Positives = 66/86 (76%)
Frame = +1 / +1
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
G V GAVETC SFHRY +Y+NFSHP I +K +P+ CGWA+GMN FDL +WR+ +T Y
Sbjct: 346 GKVNGAVETCFGSFHRYWQYMNFSHPLIKAKFNPNACGWAYGMNFFDLNSWRREKSTEQY 525
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFY 264
HYWQ QN + LLW+ GTLP GL+TFY
Sbjct: 526 HYWQTQNENRLLWKLGTLPPGLITFY 603
>gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ006_P20 5', mRNA sequence
Length = 417
Score = 151 bits (325), Expect = 2e-036
Identities = 51/79 (64%), Positives = 63/79 (79%)
Frame = +1 / +3
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
GNV+GAVETC+E+FHR+HKYLN SHP I + DP CGWAFGMN+ DL+ WR N T +Y
Sbjct: 180 GNVMGAVETCMETFHRFHKYLNHSHPLIRAHFDPDACGWAFGMNVLDLVEWRNKNVTGIY 359
Query: 187 HYWQEQNSDLLLWRTGTLP 243
HYWQE+N+D LW+ G+LP
Sbjct: 360 HYWQERNADHTLWKLGSLP 416
>gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19o15 3', mRNA sequence
Length = 635
Score = 147 bits (315), Expect = 6e-035
Identities = 49/97 (50%), Positives = 72/97 (74%)
Frame = +1 / -2
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVH 363
YH+WQ+ N D LLW+ GTLP GL+TF+ PLDR WHVLGLGY+ + R +E AAV+H
Sbjct: 634 YHFWQKLNEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIH 455
Query: 364 YNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECMLH 474
YNGN+KPWL++ + +++ W +Y++ P++REC ++
Sbjct: 454 YNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLRECNIN 344
>gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. vulgare cDNA clone HV09J15
5-PRIME, mRNA sequence
Length = 586
Score = 142 bits (304), Expect = 2e-033
Identities = 55/58 (94%), Positives = 56/58 (96%)
Frame = +1 / +2
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATS 180
GNVIGAVETCLESFHRYHKYLNFS P ISSKIDP+TCGWAFGMNIFDLIAWRKANATS
Sbjct: 413 GNVIGAVETCLESFHRYHKYLNFSQPIISSKIDPYTCGWAFGMNIFDLIAWRKANATS 586
Score = 116 bits (249), Expect = 8e-026
Identities = 49/58 (84%), Positives = 53/58 (91%)
Frame = -2 / -2
Query: 179 DVAFAFLQAIKSKMFIPKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
DVAFAFLQAI+S +FIPKAHPQV GSI ELI+GCEK RYL*YR*NDSK VSTAP+TLP
Sbjct: 585 DVAFAFLQAIRSNIFIPKAHPQVYGSIFELIIGCEKLRYL*YR*NDSKHVSTAPITLP 412
>gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 3', mRNA sequence
Length = 527
Score = 141 bits (302), Expect = 4e-033
Identities = 48/93 (51%), Positives = 68/93 (73%)
Frame = +1 / -2
Query: 187 HYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHY 366
+YWQ QN + LLW+ GTLP GL+TFY +PLD+ WHVLGLGY+ I I +AAVVH+
Sbjct: 526 NYWQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHF 347
Query: 367 NGNMKPWLKLAIRRYKYIWERYVNISHPYVREC 465
NGNMKPWL + + +++ +W +YV+ ++R+C
Sbjct: 346 NGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 248
>gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 5', mRNA sequence
Length = 586
Score = 140 bits (301), Expect = 5e-033
Identities = 48/92 (52%), Positives = 67/92 (72%)
Frame = +1 / +2
Query: 190 YWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYN 369
YWQ QN + LLW+ GTLP GL+TFY +PLD+ WHVLGLGY+ I I +AAVVH+N
Sbjct: 5 YWQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFN 184
Query: 370 GNMKPWLKLAIRRYKYIWERYVNISHPYVREC 465
GNMKPWL + + +++ +W +YV+ ++R+C
Sbjct: 185 GNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 280
>gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 5', mRNA sequence
Length = 589
Score = 140 bits (301), Expect = 5e-033
Identities = 48/92 (52%), Positives = 67/92 (72%)
Frame = +1 / +2
Query: 190 YWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYN 369
YWQ QN + LLW+ GTLP GL+TFY +PLD+ WHVLGLGY+ I I +AAVVH+N
Sbjct: 8 YWQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFN 187
Query: 370 GNMKPWLKLAIRRYKYIWERYVNISHPYVREC 465
GNMKPWL + + +++ +W +YV+ ++R+C
Sbjct: 188 GNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 283
>gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 3', mRNA sequence
Length = 525
Score = 140 bits (301), Expect = 5e-033
Identities = 48/92 (52%), Positives = 67/92 (72%)
Frame = +1 / -1
Query: 190 YWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYN 369
YWQ QN + LLW+ GTLP GL+TFY +PLD+ WHVLGLGY+ I I +AAVVH+N
Sbjct: 522 YWQTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFN 343
Query: 370 GNMKPWLKLAIRRYKYIWERYVNISHPYVREC 465
GNMKPWL + + +++ +W +YV+ ++R+C
Sbjct: 342 GNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 247
>gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25o11 3', mRNA sequence
Length = 495
Score = 132 bits (282), Expect = 2e-030
Identities = 45/90 (50%), Positives = 66/90 (73%)
Frame = +1 / -1
Query: 205 NSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKP 384
N D LLW+ GTLP GL+TF+ PLDR WHVLGLGY+ + R +E AAV+HYNGN+KP
Sbjct: 495 NEDGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLKP 316
Query: 385 WLKLAIRRYKYIWERYVNISHPYVRECMLH 474
WL++ + +++ W +Y++ P++REC ++
Sbjct: 315 WLEIGLPKFRSYWSKYLDYDQPFLRECNIN 226
>gb|AJ432742.1|AJ432742 AJ432742 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200078D03F1, mRNA sequence
Length = 447
Score = 121 bits (259), Expect = 3e-027
Identities = 44/74 (59%), Positives = 55/74 (74%)
Frame = +1 / +1
Query: 250 LLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIRRYKYIWER 429
L+TFY L PLD+ WHVLGLGY+ ID I++AAV HYNGNMKPWL+LA+ +Y+ W R
Sbjct: 7 LMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTR 186
Query: 430 YVNISHPYVRECML 471
Y+ HPY+R C L
Sbjct: 187 YIKYDHPYIRGCNL 228
>gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. vulgare cDNA clone HA17H16
5-PRIME, mRNA sequence
Length = 554
Score = 120 bits (257), Expect = 6e-027
Identities = 43/69 (62%), Positives = 52/69 (75%)
Frame = +1 / +3
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
GNV GAVETC ESFHR+ KYLNFS+P IS DP+ CGWA+GMN+FDL W+K + T +Y
Sbjct: 345 GNVNGAVETCGESFHRFDKYLNFSNPNISQNFDPNACGWAYGMNMFDLEEWKKKDITGIY 524
Query: 187 HYWQEQNSD 213
H WQ N +
Sbjct: 525 HKWQNMNEN 551
>gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 3', mRNA sequence
Length = 703
Score = 119 bits (255), Expect = 1e-026
Identities = 43/77 (55%), Positives = 55/77 (71%)
Frame = +1 / -1
Query: 127 FGMNIFDLIAWRKANATSLYHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLG 306
FGMN+ DL+ WR N T +YHYWQE+N+D LW+ G+LP GLL FYGL+E L+ +WHVLG
Sbjct: 703 FGMNVLDLVEWRNKNVTGIYHYWQERNADHTLWKLGSLPPGLLAFYGLVEALNPKWHVLG 524
Query: 307 LGYDVDIDDRLIESAAV 357
LGY D + E A +
Sbjct: 523 LGYTTVDPDTIKEGAVL 473
>gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. vulgare cDNA clone HB08B19
5-PRIME, mRNA sequence
Length = 521
Score = 92.3 bits (195), Expect(2) = 6e-026
Identities = 35/57 (61%), Positives = 42/57 (73%)
Frame = +1 / +2
Query: 7 GNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANAT 177
G V GAV TC ESFHR+ KYLNFS+P I+ DP+ CGWA+GMNIFDL W+ + T
Sbjct: 272 GMVNGAVFTCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDIT 442
Score = 44.6 bits (91), Expect(2) = 6e-026
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +1 / +3
Query: 181 LYHYWQEQNSDLLLWRTGTLPAGLL 255
+YH WQ N D +LW+ GTLP GL+
Sbjct: 447 IYHKWQNMNEDRVLWKLGTLPPGLM 521
>gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. vulgare cDNA clone HM10N01
3-PRIME, mRNA sequence
Length = 535
Score = 116 bits (249), Expect = 8e-026
Identities = 40/78 (51%), Positives = 57/78 (73%)
Frame = +1 / -3
Query: 232 GTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIRRY 411
GTLP GL+TFY +PLD+ WHVLGLGY+ I I +AAVVH+NGNMKPWL + + ++
Sbjct: 530 GTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMKPWLDIGMNQF 351
Query: 412 KYIWERYVNISHPYVREC 465
+ +W +YV+ ++R+C
Sbjct: 350 RQLWTKYVDYDDSFIRQC 297
>gb|BU986456.1|BU986456 HF11O19r HF Hordeum vulgare subsp. vulgare cDNA clone HF11O19
5-PRIME, mRNA sequence
Length = 478
Score = 114 bits (244), Expect = 4e-025
Identities = 41/82 (50%), Positives = 57/82 (69%)
Frame = +1 / +3
Query: 220 LWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLA 399
L T LPAGL+TFYG +PLD+ WHV+GLGY+ I +I AAV+H+NGNMKPWL +A
Sbjct: 27 LGSTSVLPAGLMTFYGNTKPLDKSWHVMGLGYNPSISPEVIRGAAVIHFNGNMKPWLDVA 206
Query: 400 IRRYKYIWERYVNISHPYVREC 465
+YK +W ++V+ ++ C
Sbjct: 207 FNQYKALWTKHVDTEMEFLTLC 272
>gb|AV834161.1|AV834161 AV834161 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags5h22, mRNA sequence
Length = 714
Score = 91.8 bits (194), Expect(2) = 3e-024
Identities = 38/90 (42%), Positives = 52/90 (57%)
Frame = +1 / -1
Query: 196 QEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGN 375
Q + D + R LL+F L+ PLD R + GLGYD ID + +S+A +HYNGN
Sbjct: 603 QVKKKDEVSLREAAFSLSLLSFQNLIYPLDGRSTLSGLGYDYGIDPEVAQSSAALHYNGN 424
Query: 376 MKPWLKLAIRRYKYIWERYVNISHPYVREC 465
MKPWL+L I YK W R++ ++ EC
Sbjct: 423 MKPWLELGIPDYKKYWRRFLTREDRFMDEC 334
Score = 39.6 bits (80), Expect(2) = 3e-024
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +1 / -1
Query: 94 SKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
+K DP +C W G+N+ +L WRK T Y
Sbjct: 711 TKYDPKSCAWMSGVNVINLDKWRKHKVTENY 619
>gb|BQ460226.1|BQ460226 HA06N23r HA Hordeum vulgare subsp. vulgare cDNA clone HA06N23
5-PRIME, mRNA sequence
Length = 567
Score = 105 bits (225), Expect = 2e-022
Identities = 38/84 (45%), Positives = 53/84 (63%)
Frame = +1 / +2
Query: 214 LLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLK 393
L LW+ GTLP L+ F G + +D WH+LGLGY D ++ AAVVHYNG KPWL
Sbjct: 17 LTLWKFGTLPPALIAFRGHVHGIDPSWHMLGLGYQESTDIESVKKAAVVHYNGQCKPWLD 196
Query: 394 LAIRRYKYIWERYVNISHPYVREC 465
+A + + W ++VN S+ ++R C
Sbjct: 197 IAFKNLQPFWTKHVNYSNDFIRNC 268
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 299,544,681
Number of Sequences: 312970
Number of extensions: 4588417
Number of successful extensions: 47742
Number of sequences better than 1.0e-020: 49
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6277672932
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)