TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3336981.2.1
(510 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE216598.1|BE216598 HV_CEb0010O18f Hordeum vulgare seedl... 171 2e-042
gb|BG310018.1|BG310018 HVSMEc0016B06f Hordeum vulgare seedl... 147 2e-041
gb|BI777732.2|BI777732 EBro08_SQ001_L03_R root, 3 week, dro... 132 3e-039
gb|BQ468452.1|BQ468452 HM01E18T HM Hordeum vulgare subsp. v... 145 1e-034
gb|BJ449299.1|BJ449299 BJ449299 K. Sato unpublished cDNA li... 118 1e-033
gb|BJ452141.1|BJ452141 BJ452141 K. Sato unpublished cDNA li... 105 1e-029
gb|BE216587.2|BE216587 HV_CEb0010O06f Hordeum vulgare seedl... 102 1e-021
>gb|BE216598.1|BE216598 HV_CEb0010O18f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0010O18f, mRNA sequence
Length = 725
Score = 171 bits (368), Expect = 2e-042
Identities = 67/141 (47%), Positives = 95/141 (67%)
Frame = +1 / +1
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVI+NTISA P
Sbjct: 52 YTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTISALP 231
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIV 447
+L + L G + Y++ L G H+++F IA+V+P+FLMGII+
Sbjct: 232 FLVLICFLSGTVCYFMVRLHPGFSHYIFFVLNLYASVTVVESLMMAIASVIPNFLMGIII 411
Query: 448 GAGVQGVMMLNGGFFRLPNEL 510
GAG+QG+ ML G+FRLP ++
Sbjct: 412 GAGIQGIFMLVSGYFRLPYDI 474
>gb|BG310018.1|BG310018 HVSMEc0016B06f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0016B06f, mRNA sequence
Length = 808
Score = 147 bits (315), Expect(2) = 2e-041
Identities = 61/143 (42%), Positives = 91/143 (63%)
Frame = +1 / +3
Query: 82 STYSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISA 261
++Y++IQ+R + +TFMSIGGFPSF+E++KVF ER +GHYGV+ ++ISN +S+
Sbjct: 78 NSYTAIQARASCGGFVSGFMTFMSIGGFPSFIEEMKVFSLERQNGHYGVAAYIISNFLSS 257
Query: 262 TPYLAGVSLLPGAMLYYLTGLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGI 441
P+L +S ++ Y++ G +F +F I+A+VP+FLMG+
Sbjct: 258 MPFLLTMSWASASITYWMVKFRPGFSYFAFFALNLYGGVSVIESLMMIISALVPNFLMGL 437
Query: 442 IVGAGVQGVMMLNGGFFRLPNEL 510
I+GAGV G+MML GFFRL EL
Sbjct: 438 ILGAGVIGIMMLTSGFFRLLPEL 506
Score = 41.4 bits (84), Expect(2) = 2e-041
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = +1 / +3
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLGYS 84
GYY +R+ IY+ + +CLGTI+Y +G S
Sbjct: 3 GYYRLRIIIYVLMAICLGTIYYDVGNS 83
>gb|BI777732.2|BI777732 EBro08_SQ001_L03_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ001_L03 5', mRNA sequence
Length = 447
Score = 132 bits (283), Expect(2) = 3e-039
Identities = 52/78 (66%), Positives = 66/78 (84%)
Frame = +1 / +1
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y SIQ+R ++M+ A LTFM+IGGFPSFVED+K+F +ERL+GHYGVS FVI+NT+S+TP
Sbjct: 214 YGSIQARGSMLMFVGAFLTFMAIGGFPSFVEDMKIFGRERLNGHYGVSSFVIANTVSSTP 393
Query: 268 YLAGVSLLPGAMLYYLTG 321
YL +SL+PGAM YYL G
Sbjct: 394 YLLLISLVPGAMAYYLVG 447
Score = 48.3 bits (99), Expect(2) = 3e-039
Identities = 15/26 (57%), Positives = 21/26 (80%)
Frame = +1 / +1
Query: 1 VGYYWMRLAIYMGIGVCLGTIFYQLG 78
+GYYW+R AIY+ + +C GTIFY +G
Sbjct: 130 LGYYWLRFAIYIALCLCCGTIFYDIG 207
>gb|BQ468452.1|BQ468452 HM01E18T HM Hordeum vulgare subsp. vulgare cDNA clone HM01E18
5-PRIME, mRNA sequence
Length = 620
Score = 145 bits (312), Expect = 1e-034
Identities = 56/116 (48%), Positives = 80/116 (68%)
Frame = +1 / +2
Query: 163 FPSFVEDVKVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLTGLSKGADH 342
FPSFVE++KVF++ERL+GHYGV+ FVI+NTISA P+L + L G + Y++ L G H
Sbjct: 2 FPSFVEEMKVFQRERLNGHYGVAAFVIANTISALPFLVLICFLSGTVCYFMVRLHPGFSH 181
Query: 343 FVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQGVMMLNGGFFRLPNEL 510
+++F IA+V+P+FLMGII+GAG+QG+ ML G+FRLP ++
Sbjct: 182 YIFFVLNLYASVTVVESLMMAIASVIPNFLMGIIIGAGIQGIFMLVSGYFRLPYDI 349
>gb|BJ449299.1|BJ449299 BJ449299 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak23f10 5', mRNA sequence
Length = 652
Score = 118 bits (252), Expect(2) = 1e-033
Identities = 45/89 (50%), Positives = 64/89 (71%)
Frame = +1 / +2
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVI+NTISA P
Sbjct: 377 YTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTISALP 556
Query: 268 YLAGVSLLPGAMLYYLTGLSKGADHFVYF 354
+L + L G + Y++ L G H+++F
Sbjct: 557 FLVLICFLSGTVCYFMVRLHPGFSHYIFF 643
Score = 44.1 bits (90), Expect(2) = 1e-033
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1 / +2
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + VC+GTI+ +G
Sbjct: 296 GYYWLRLLIYLLVTVCIGTIYLDVG 370
>gb|BJ452141.1|BJ452141 BJ452141 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak37o18 5', mRNA sequence
Length = 661
Score = 105 bits (223), Expect(2) = 1e-029
Identities = 41/75 (54%), Positives = 56/75 (74%)
Frame = +1 / +2
Query: 88 YSSIQSRCEVIMYTIALLTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATP 267
Y+SI +R + +TFMSIGGFPSFVE++KVF++ERL+GHYGV+ FVI+NTISA P
Sbjct: 437 YTSILARAACAAFVFGFVTFMSIGGFPSFVEEMKVFQRERLNGHYGVAAFVIANTISALP 616
Query: 268 YLAGVSLLPGAMLYY 312
+L + L G + Y+
Sbjct: 617 FLVLICFLSGTVCYF 661
Score = 44.1 bits (90), Expect(2) = 1e-029
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +1 / +2
Query: 4 GYYWMRLAIYMGIGVCLGTIFYQLG 78
GYYW+RL IY+ + VC+GTI+ +G
Sbjct: 356 GYYWLRLLIYLLVTVCIGTIYLDVG 430
>gb|BE216587.2|BE216587 HV_CEb0010O06f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0010O06f, mRNA sequence
Length = 770
Score = 102 bits (218), Expect = 1e-021
Identities = 44/109 (40%), Positives = 62/109 (56%)
Frame = +1 / +2
Query: 139 LTFMSIGGFPSFVEDVKVFRKERLSGHYGVSEFVISNTISATPYLAGVSLLPGAMLYYLT 318
L F+ I G PSF E + VF+ ERL+G Y V+ VI+NTIS P+L + L G + Y++
Sbjct: 101 LIFIPIAGLPSFAEQMNVFQWERLNGPYSVAAIVIANTISDLPFLDLICFLSGTVCYFMV 280
Query: 319 GLSKGADHFVYFXXXXXXXXXXXXXXXXXIAAVVPDFLMGIIVGAGVQG 465
L G H++ F IA+ +P+FLMGII+GA +QG
Sbjct: 281 RLHPGFSHYILFVLNLYASVTVVESLMMAIASGIPNFLMGIIIGAEIQG 427
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 204,091,061
Number of Sequences: 312970
Number of extensions: 2570342
Number of successful extensions: 75424
Number of sequences better than 1.0e-020: 9
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5084831801
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)