TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3230315.2.1
(457 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 221 1e-057
gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA li... 200 4e-051
gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no... 198 2e-050
gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. v... 123 4e-034
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 144 4e-034
gb|AV919291.1|AV919291 AV919291 K. Sato unpublished cDNA li... 118 1e-026
gb|BG416061.2|BG416061 HVSMEk0009J20f Hordeum vulgare testa... 98 2e-026
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 110 6e-024
gb|BG417076.2|BG417076 HVSMEk0016B22f Hordeum vulgare testa... 87 2e-023
gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. v... 101 2e-021
gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. v... 101 2e-021
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 221 bits (478), Expect = 1e-057
Identities = 85/102 (83%), Positives = 97/102 (95%)
Frame = -1 / +3
Query: 451 AKTYLGRPWKQYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGR 272
A++YLGRPWKQYSRTV++QSELDS+V+PAGWL W+G+FALDTLYYGEY NTGPGAGTSGR
Sbjct: 111 ARSYLGRPWKQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGR 290
Query: 271 VKWKGYRVITSAAEASAFTVGNFIDGDLWLAGTSIPFTTGL* 146
V WKGYRVITSA+EAS FTVG+FIDGD+WLAGTSIPF+ GL*
Sbjct: 291 VTWKGYRVITSASEASTFTVGSFIDGDVWLAGTSIPFSAGL* 416
Score = 188 bits (406), Expect = 1e-047
Identities = 80/99 (80%), Positives = 84/99 (84%)
Frame = +3 / -3
Query: 147 HKPVVKGMDVPASQRSPSMKLPTVKALASAALVMTRYPFHLTRPDVPAPGPVFMYSP**S 326
H P GMDVPASQ SPSMK PTVK LAS ALVMTRYPFH+T PDVPAPGPVF YSP**S
Sbjct: 415 HSPAENGMDVPASQTSPSMKEPTVKVLASDALVMTRYPFHVTLPDVPAPGPVFWYSP**S 236
Query: 327 VSSAKLPFHSSQPAGSTSESSSDCRYTVREYCFHGRPR* 443
VSSAK+P H+SQPAG T+ SSSDC TVREYCFHGRPR*
Sbjct: 235 VSSAKVPSHASQPAGLTTLSSSDCMKTVREYCFHGRPR* 119
>gb|BJ468665.1|BJ468665 BJ468665 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 3', mRNA sequence
Length = 606
Score = 200 bits (431), Expect = 4e-051
Identities = 78/93 (83%), Positives = 88/93 (94%)
Frame = -1 / -3
Query: 424 KQYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGRVKWKGYRVI 245
KQYSRTV++QSELDS+V+PAGWL W+G+FALDTLYYGEY NTGPGAGTSGRV WKGYRVI
Sbjct: 604 KQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVI 425
Query: 244 TSAAEASAFTVGNFIDGDLWLAGTSIPFTTGL* 146
TSA+EAS FTVG+FIDGD+WLAGTSIPF+ GL*
Sbjct: 424 TSASEASTFTVGSFIDGDVWLAGTSIPFSAGL* 326
Score = 174 bits (374), Expect = 3e-043
Identities = 74/93 (79%), Positives = 78/93 (83%)
Frame = +3 / +3
Query: 147 HKPVVKGMDVPASQRSPSMKLPTVKALASAALVMTRYPFHLTRPDVPAPGPVFMYSP**S 326
H P GMDVPASQ SPSMK PTVK LAS ALVMTRYPFH+T PDVPAPGPVF YSP**S
Sbjct: 327 HSPAENGMDVPASQTSPSMKEPTVKVLASDALVMTRYPFHVTLPDVPAPGPVFWYSP**S 506
Query: 327 VSSAKLPFHSSQPAGSTSESSSDCRYTVREYCF 425
VSSAK+P H+SQPAG T+ SSSDC TVREYCF
Sbjct: 507 VSSAKVPSHASQPAGLTTLSSSDCMKTVREYCF 605
>gb|BM374246.2|BM374246 EBpi03_SQ003_B10_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_B10 5', mRNA sequence
Length = 449
Score = 198 bits (426), Expect = 2e-050
Identities = 77/92 (83%), Positives = 87/92 (94%)
Frame = -1 / +2
Query: 421 QYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGRVKWKGYRVIT 242
QYSRTV++QSELDS+V+PAGWL W+G+FALDTLYYGEY NTGPGAGTSGRV WKGYRVIT
Sbjct: 2 QYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVTWKGYRVIT 181
Query: 241 SAAEASAFTVGNFIDGDLWLAGTSIPFTTGL* 146
SA+EAS FTVG+FIDGD+WLAGTSIPF+ GL*
Sbjct: 182 SASEASTFTVGSFIDGDVWLAGTSIPFSAGL* 277
Score = 171 bits (368), Expect = 2e-042
Identities = 73/92 (79%), Positives = 77/92 (83%)
Frame = +3 / -3
Query: 147 HKPVVKGMDVPASQRSPSMKLPTVKALASAALVMTRYPFHLTRPDVPAPGPVFMYSP**S 326
H P GMDVPASQ SPSMK PTVK LAS ALVMTRYPFH+T PDVPAPGPVF YSP**S
Sbjct: 276 HSPAENGMDVPASQTSPSMKEPTVKVLASDALVMTRYPFHVTLPDVPAPGPVFWYSP**S 97
Query: 327 VSSAKLPFHSSQPAGSTSESSSDCRYTVREYC 422
VSSAK+P H+SQPAG T+ SSSDC TVREYC
Sbjct: 96 VSSAKVPSHASQPAGLTTLSSSDCMKTVREYC 1
>gb|BU990192.1|BU990192 HF24F16r HF Hordeum vulgare subsp. vulgare cDNA clone HF24F16
5-PRIME, mRNA sequence
Length = 463
Score = 123 bits (263), Expect(2) = 4e-034
Identities = 46/60 (76%), Positives = 49/60 (81%)
Frame = -1 / +2
Query: 442 YLGRPWKQYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGRVKW 263
YLGRPWK YSRTV + S LD + PAGWLEW+G FAL TLYYGEY NTGPGAGT GRVKW
Sbjct: 5 YLGRPWKAYSRTVVMGSSLDRWIAPAGWLEWSGQFALSTLYYGEYGNTGPGAGTGGRVKW 184
Score = 40.0 bits (81), Expect(2) = 4e-034
Identities = 16/32 (50%), Positives = 22/32 (68%)
Frame = -1 / +2
Query: 241 SAAEASAFTVGNFIDGDLWLAGTSIPFTTGL* 146
S +A+ FTV +FI GD WL T + +T+GL*
Sbjct: 197 STVDATRFTVRDFILGDSWLGDTGVSYTSGL* 292
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 144 bits (308), Expect = 4e-034
Identities = 53/93 (56%), Positives = 65/93 (69%)
Frame = -1 / +2
Query: 427 WKQYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGRVKWKGYRV 248
WK YSRTV +QS++ +VDPAGW+ W+G FAL TLYY EY N+G G+ TS RV W GY V
Sbjct: 2 WKMYSRTVIMQSDVAGIVDPAGWMPWSGDFALSTLYYAEYDNSGAGSDTSRRVNWPGYHV 181
Query: 247 ITSAAEASAFTVGNFIDGDLWLAGTSIPFTTGL 149
+ S +A FTV N + GD WL T +PFT GL
Sbjct: 182 LNSTVDAGNFTVANMVLGDFWLPQTGVPFTVGL 280
>gb|AV919291.1|AV919291 AV919291 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags5g01 3', mRNA sequence
Length = 339
Score = 118 bits (253), Expect = 1e-026
Identities = 47/55 (85%), Positives = 51/55 (92%)
Frame = -1 / -1
Query: 310 YMNTGPGAGTSGRVKWKGYRVITSAAEASAFTVGNFIDGDLWLAGTSIPFTTGL* 146
Y NTGPGAGTSGRV WKGYRVITSA+EAS FTVG+FIDGD+WLAGTSIPF+ GL*
Sbjct: 339 YQNTGPGAGTSGRVTWKGYRVITSASEASTFTVGSFIDGDVWLAGTSIPFSAGL* 175
Score = 105 bits (223), Expect = 2e-022
Identities = 43/53 (81%), Positives = 44/53 (83%)
Frame = +3 / +2
Query: 147 HKPVVKGMDVPASQRSPSMKLPTVKALASAALVMTRYPFHLTRPDVPAPGPVF 305
H P GMDVPASQ SPSMK PTVK LAS ALVMTRYPFH+T PDVPAPGPVF
Sbjct: 176 HSPAENGMDVPASQTSPSMKEPTVKVLASDALVMTRYPFHVTLPDVPAPGPVF 334
>gb|BG416061.2|BG416061 HVSMEk0009J20f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009J20f, mRNA sequence
Length = 465
Score = 98.2 bits (208), Expect(2) = 2e-026
Identities = 37/50 (74%), Positives = 40/50 (80%)
Frame = -1 / +2
Query: 412 RTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGRVKW 263
RTV + S LD + PAGWLEW+G FAL TLYYGEY NTGPGAGT GRVKW
Sbjct: 2 RTVVMGSSLDRWIAPAGWLEWSGQFALSTLYYGEYGNTGPGAGTGGRVKW 151
Score = 39.1 bits (79), Expect(2) = 2e-026
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = -1 / +2
Query: 241 SAAEASAFTVGNFIDGDLWLAGTSIPFTTGL* 146
S +A+ FTV +FI GD W+ T + +T+GL*
Sbjct: 164 STVDATRFTVRDFILGDSWMGDTGVSYTSGL* 259
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 110 bits (234), Expect = 6e-024
Identities = 39/67 (58%), Positives = 50/67 (74%)
Frame = -1 / +2
Query: 445 TYLGRPWKQYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGRVK 266
+YLGRPWK++SR V ++S++ + P G++ WNG F L TLYY EY N GPGAGTS RV
Sbjct: 314 SYLGRPWKEFSRLVIMESQIADFIKPEGYMPWNGDFGLKTLYYAEYGNRGPGAGTSKRVT 493
Query: 265 WKGYRVI 245
W G+RVI
Sbjct: 494 WPGFRVI 514
>gb|BG417076.2|BG417076 HVSMEk0016B22f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016B22f, mRNA sequence
Length = 401
Score = 86.8 bits (183), Expect(2) = 2e-023
Identities = 31/36 (86%), Positives = 32/36 (88%)
Frame = -1 / +3
Query: 370 PAGWLEWNGSFALDTLYYGEYMNTGPGAGTSGRVKW 263
PAGWLEW+G FAL TLYYGEY NTGPGAGT GRVKW
Sbjct: 3 PAGWLEWSGQFALSTLYYGEYGNTGPGAGTGGRVKW 110
Score = 40.0 bits (81), Expect(2) = 2e-023
Identities = 16/32 (50%), Positives = 22/32 (68%)
Frame = -1 / +3
Query: 241 SAAEASAFTVGNFIDGDLWLAGTSIPFTTGL* 146
S +A+ FTV +FI GD WL T + +T+GL*
Sbjct: 123 STVDATRFTVRDFILGDSWLGDTGVSYTSGL* 218
>gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09G17
3-PRIME, mRNA sequence
Length = 565
Score = 101 bits (216), Expect = 2e-021
Identities = 36/57 (63%), Positives = 45/57 (78%)
Frame = -1 / -1
Query: 448 KTYLGRPWKQYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTS 278
+ +LGRPWK+YSRTV++ L +V PAGW+ W+G FALDTLYYGEY + GPGAG S
Sbjct: 382 RVFLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRS 212
>gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. vulgare cDNA clone HI12E03
3-PRIME, mRNA sequence
Length = 521
Score = 101 bits (216), Expect = 2e-021
Identities = 36/57 (63%), Positives = 45/57 (78%)
Frame = -1 / -1
Query: 448 KTYLGRPWKQYSRTVYLQSELDSLVDPAGWLEWNGSFALDTLYYGEYMNTGPGAGTS 278
+ +LGRPWK+YSRTV++ L +V PAGW+ W+G FALDTLYYGEY + GPGAG S
Sbjct: 383 RVFLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRS 213
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 183,050,975
Number of Sequences: 312970
Number of extensions: 2552729
Number of successful extensions: 113978
Number of sequences better than 1.0e-020: 14
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 4315697579
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)