TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3204398.2.1
(666 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedl... 193 6e-049
gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. v... 192 2e-048
gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots... 191 4e-048
gb|BF259816.3|BF259816 HVSMEf0020F12f Hordeum vulgare seedl... 147 5e-035
gb|AL502881.1|AL502881 AL502881 Hordeum vulgare Barke roots... 124 7e-033
gb|BF258077.2|BF258077 HVSMEf0014L07f Hordeum vulgare seedl... 72 8e-022
>gb|BE559029.2|BE559029 HV_CEb0022G04f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0022G04f, mRNA sequence
Length = 669
Score = 193 bits (417), Expect = 6e-049
Identities = 80/89 (89%), Positives = 86/89 (96%)
Frame = +1 / +3
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSGNFE AL+ ILRCAENPAKYFAK+LRK+MKGLGTDDKTL+RVVVTRTEIDMQYIKAEY
Sbjct: 153 TSGNFEVALITILRCAENPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEY 332
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVG 267
+KKYKKPLA+AIHSETSG YRTFLLSLVG
Sbjct: 333 YKKYKKPLADAIHSETSGGYRTFLLSLVG 419
Score = 163 bits (351), Expect = 7e-040
Identities = 73/88 (82%), Positives = 74/88 (84%)
Frame = -2 / -3
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMA 87
PT E RNVR* PDVSEWIASANGFLYFL YSALIYCISISVLVTTTL SVLSSVPRPFM
Sbjct: 418 PTRESRNVR*PPDVSEWIASANGFLYFL*YSALIYCISISVLVTTTLTSVLSSVPRPFMD 239
Query: 86 FRKSFAKYFAGFSAHLRIAKSANSKFPD 3
F + AKY AGFSAHLRI A SKFPD
Sbjct: 238 FLNTLAKYLAGFSAHLRIVIRATSKFPD 155
Score = 133 bits (286), Expect = 7e-031
Identities = 56/88 (63%), Positives = 73/88 (82%)
Frame = +3 / +2
Query: 3 IWKL*IRTFSYPQMRGKPSKVFCKALTKGHERSRH**QDTYKGCGDED*N*YAIYQGRVL 182
IWKL* + +YPQM + S VFC + + HERSR+**QDT KGCG+ED*+*YA+YQGR+L
Sbjct: 155 IWKL*SCSDNYPQMC*ESS*VFC*GVKEVHERSRY**QDTCKGCGNED*D*YAVYQGRIL 334
Query: 183 QEV*EAIS*GYPLRNIRKLSDIPSFTCW 266
QE+ +A+S* YP RNIR+LSD+P+F+CW
Sbjct: 335 QEIQKAVS*CYPFRNIRRLSDVPAFSCW 418
Score = 104 bits (222), Expect = 5e-022
Identities = 54/88 (61%), Positives = 59/88 (67%)
Frame = -3 / -1
Query: 265 QQVKEGMSDSFLMFRSG*PQLMASYTS*STLP*YIAYQFQSSSPQPL*VSCHQCLDLSWP 86
QQ K G SD+ LMF +G* QL A S S LP*Y AYQ QSS PQPL VSCHQ LDLSW
Sbjct: 417 QQEKAGTSDNLLMFLNG*HQLTAFCISCSILP*YTAYQSQSSLPQPLQVSCHQYLDLSWT 238
Query: 85 FVRALQNTLLGFPRI*G*LKVRIQSFQM 2
+ QNT L I*G*L ++QSFQM
Sbjct: 237 SLTP*QNT*LDSQHI*G*LSEQLQSFQM 154
>gb|BQ663389.1|BQ663389 HU02G23u HU Hordeum vulgare subsp. vulgare cDNA clone HU02G23
3-PRIME, mRNA sequence
Length = 495
Score = 192 bits (413), Expect = 2e-048
Identities = 79/89 (88%), Positives = 86/89 (96%)
Frame = +1 / -2
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSGNFE AL+ ILRCAENPAKYFAK+LRK+MKGLGTDDKTL+RVVVTRTEIDMQYIKAEY
Sbjct: 386 TSGNFEVALITILRCAENPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEY 207
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLVG 267
+KKYKKPLA+AIHS+TSG YRTFLLSLVG
Sbjct: 206 YKKYKKPLADAIHSKTSGGYRTFLLSLVG 120
Score = 160 bits (345), Expect = 5e-039
Identities = 72/88 (81%), Positives = 73/88 (82%)
Frame = -2 / +1
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMA 87
PT E RNVR* PDV EWIASANGFLYFL YSALIYCISISVLVTTTL SVLSSVPRPFM
Sbjct: 121 PTRESRNVR*PPDVFEWIASANGFLYFL*YSALIYCISISVLVTTTLTSVLSSVPRPFMD 300
Query: 86 FRKSFAKYFAGFSAHLRIAKSANSKFPD 3
F + AKY AGFSAHLRI A SKFPD
Sbjct: 301 FLNTLAKYLAGFSAHLRIVIRATSKFPD 384
Score = 132 bits (283), Expect = 2e-030
Identities = 55/88 (62%), Positives = 73/88 (82%)
Frame = +3 / -1
Query: 3 IWKL*IRTFSYPQMRGKPSKVFCKALTKGHERSRH**QDTYKGCGDED*N*YAIYQGRVL 182
IWKL* + +YPQM + S VFC + + HERSR+**QDT KGCG+ED*+*YA+YQGR+L
Sbjct: 384 IWKL*SCSDNYPQMC*ESS*VFC*GVKEVHERSRY**QDTCKGCGNED*D*YAVYQGRIL 205
Query: 183 QEV*EAIS*GYPLRNIRKLSDIPSFTCW 266
QE+ +A+S* YP +NIR+LSD+P+F+CW
Sbjct: 204 QEIQKAVS*CYPFKNIRRLSDVPAFSCW 121
Score = 104 bits (222), Expect = 5e-022
Identities = 54/88 (61%), Positives = 59/88 (67%)
Frame = -3 / +2
Query: 265 QQVKEGMSDSFLMFRSG*PQLMASYTS*STLP*YIAYQFQSSSPQPL*VSCHQCLDLSWP 86
QQ K G SD+ LMF +G* QL A S S LP*Y AYQ QSS PQPL VSCHQ LDLSW
Sbjct: 122 QQEKAGTSDNLLMFLNG*HQLTAFCISCSILP*YTAYQSQSSLPQPLQVSCHQYLDLSWT 301
Query: 85 FVRALQNTLLGFPRI*G*LKVRIQSFQM 2
+ QNT L I*G*L ++QSFQM
Sbjct: 302 SLTP*QNT*LDSQHI*G*LSEQLQSFQM 385
>gb|AL505620.1|AL505620 AL505620 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09A09V 5', mRNA sequence
Length = 468
Score = 191 bits (411), Expect = 4e-048
Identities = 79/88 (89%), Positives = 85/88 (96%)
Frame = +1 / +1
Query: 1 TSGNFEFALLAILRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEY 180
TSGNFE AL+ ILRCAENPAKYFAK+LRK+MKGLGTDDKTL+RVVVTRTEIDMQYIKAEY
Sbjct: 205 TSGNFEVALITILRCAENPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEY 384
Query: 181 FKKYKKPLAEAIHSETSGNYRTFLLSLV 264
+KKYKKPLA+AIHSETSG YRTFLLSLV
Sbjct: 385 YKKYKKPLADAIHSETSGGYRTFLLSLV 468
Score = 160 bits (344), Expect = 7e-039
Identities = 72/87 (82%), Positives = 73/87 (83%)
Frame = -2 / -2
Query: 263 TSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMAF 84
T E RNVR* PDVSEWIASANGFLYFL YSALIYCISISVLVTTTL SVLSSVPRPFM F
Sbjct: 467 TRESRNVR*PPDVSEWIASANGFLYFL*YSALIYCISISVLVTTTLTSVLSSVPRPFMDF 288
Query: 83 RKSFAKYFAGFSAHLRIAKSANSKFPD 3
+ AKY AGFSAHLRI A SKFPD
Sbjct: 287 LNTLAKYLAGFSAHLRIVIRATSKFPD 207
Score = 128 bits (275), Expect = 2e-029
Identities = 55/87 (63%), Positives = 72/87 (82%)
Frame = +3 / +3
Query: 3 IWKL*IRTFSYPQMRGKPSKVFCKALTKGHERSRH**QDTYKGCGDED*N*YAIYQGRVL 182
IWKL* + +YPQM + S VFC + + HERSR+**QDT KGCG+ED*+*YA+YQGR+L
Sbjct: 207 IWKL*SCSDNYPQMC*ESS*VFC*GVKEVHERSRY**QDTCKGCGNED*D*YAVYQGRIL 386
Query: 183 QEV*EAIS*GYPLRNIRKLSDIPSFTC 263
QE+ +A+S* YP RNIR+LSD+P+F+C
Sbjct: 387 QEIQKAVS*CYPFRNIRRLSDVPAFSC 467
Score = 102 bits (217), Expect = 2e-021
Identities = 53/87 (60%), Positives = 58/87 (66%)
Frame = -3 / -3
Query: 262 QVKEGMSDSFLMFRSG*PQLMASYTS*STLP*YIAYQFQSSSPQPL*VSCHQCLDLSWPF 83
Q K G SD+ LMF +G* QL A S S LP*Y AYQ QSS PQPL VSCHQ LDLSW
Sbjct: 466 QEKAGTSDNLLMFLNG*HQLTAFCISCSILP*YTAYQSQSSLPQPLQVSCHQYLDLSWTS 287
Query: 82 VRALQNTLLGFPRI*G*LKVRIQSFQM 2
+ QNT L I*G*L ++QSFQM
Sbjct: 286 LTP*QNT*LDSQHI*G*LSEQLQSFQM 206
>gb|BF259816.3|BF259816 HVSMEf0020F12f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020F12f, mRNA sequence
Length = 502
Score = 147 bits (316), Expect = 5e-035
Identities = 62/77 (80%), Positives = 67/77 (87%)
Frame = +1 / +1
Query: 37 LRCAENPAKYFAKLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEYFKKYKKPLAEAI 216
LRCA+ PAKYFAK+L KAMKGLGT + LIRVVVTRTE+DM+YIK EY KYK LAEAI
Sbjct: 1 LRCADTPAKYFAKVLHKAMKGLGTSNAALIRVVVTRTEVDMKYIKVEYHNKYKGSLAEAI 180
Query: 217 HSETSGNYRTFLLSLVG 267
HSETSGNYRTFLLSLVG
Sbjct: 181 HSETSGNYRTFLLSLVG 231
Score = 106 bits (227), Expect = 9e-023
Identities = 50/76 (65%), Positives = 57/76 (75%)
Frame = -2 / -3
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMA 87
PTSERR V *FPDVSE IASA+ LY L YS L+Y +S SVLVTTT +S VPRPF+A
Sbjct: 230 PTSERRKVL*FPDVSECIASASDPLYLLWYSTLMYFMSTSVLVTTTRMSAALLVPRPFIA 51
Query: 86 FRKSFAKYFAGFSAHL 39
++ AKYFAG SAHL
Sbjct: 50 LCRTLAKYFAGVSAHL 3
>gb|AL502881.1|AL502881 AL502881 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW09A09u 3', mRNA sequence
Length = 658
Score = 124 bits (266), Expect(3) = 7e-033
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1 / -3
Query: 94 KGLGTDDKTLIRVVVTRTEIDMQYIKAEYFKKYKKPLAEAIHSETSGNYRTFLLSLVG 267
K GTDDKTL+RVVVTRTEIDMQYIKAEY+KKYKKPLA+AIHSETSG YRTFLLSLVG
Sbjct: 500 KVXGTDDKTLVRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSGGYRTFLLSLVG 327
Score = 113 bits (242), Expect = 8e-025
Identities = 54/76 (71%), Positives = 56/76 (73%)
Frame = -2 / +1
Query: 266 PTSERRNVR*FPDVSEWIASANGFLYFLKYSALIYCISISVLVTTTLISVLSSVPRPFMA 87
PT E RNVR* PDVSEWIASANGFLYFL YSALIYCISISVLVTTTL SVLSSVP F
Sbjct: 328 PTRESRNVR*PPDVSEWIASANGFLYFL*YSALIYCISISVLVTTTLTSVLSSVPXTFHG 507
Query: 86 FRKSFAKYFAGFSAHL 39
+K S H+
Sbjct: 508 LP*HLSKILXLDSQHI 555
Score = 110 bits (235), Expect = 7e-024
Identities = 44/69 (63%), Positives = 57/69 (82%)
Frame = +3 / -2
Query: 60 KVFCKALTKGHERSRH**QDTYKGCGDED*N*YAIYQGRVLQEV*EAIS*GYPLRNIRKL 239
K F K L K + R+**QDT KGCG+ED*+*YA+YQGR+LQE+ +A+S* YP RNIR+L
Sbjct: 534 KYFAKVLRKSMKGXRY**QDTCKGCGNED*D*YAVYQGRILQEIQKAVS*CYPFRNIRRL 355
Query: 240 SDIPSFTCW 266
SD+P+F+CW
Sbjct: 354 SDVPAFSCW 328
Score = 29.9 bits (59), Expect(3) = 7e-033
Identities = 11/13 (84%), Positives = 13/13 (100%)
Frame = +1 / -2
Query: 61 KYFAKLLRKAMKG 99
KYFAK+LRK+MKG
Sbjct: 534 KYFAKVLRKSMKG 496
Score = 23.5 bits (45), Expect(3) = 7e-033
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / -1
Query: 37 LRCAENPAKYF 69
LRCAENPA F
Sbjct: 559 LRCAENPA*VF 527
>gb|BF258077.2|BF258077 HVSMEf0014L07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0014L07f, mRNA sequence
Length = 573
Score = 71.6 bits (150), Expect(2) = 8e-022
Identities = 29/41 (70%), Positives = 34/41 (82%)
Frame = +1 / +2
Query: 73 KLLRKAMKGLGTDDKTLIRVVVTRTEIDMQYIKAEYFKKYK 195
++L KAMKGLGT + LIRVVVTRTE+DM+YIK EY KYK
Sbjct: 443 QVLHKAMKGLGTSNAALIRVVVTRTEVDMKYIKVEYHNKYK 565
Score = 51.5 bits (106), Expect(2) = 8e-022
Identities = 19/25 (76%), Positives = 22/25 (88%)
Frame = +1 / +3
Query: 1 TSGNFEFALLAILRCAENPAKYFAK 75
T+GNF+F LL ILRCA+ PAKYFAK
Sbjct: 372 TAGNFQFGLLTILRCADTPAKYFAK 446
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 289,994,905
Number of Sequences: 312970
Number of extensions: 4127674
Number of successful extensions: 31290
Number of sequences better than 1.0e-020: 8
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7210840530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)