TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198833.2.1
(593 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike... 418 e-117
gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA,... 400 e-111
gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA li... 162 1e-039
gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthe... 158 3e-038
gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachi... 98 1e-034
gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachi... 98 3e-034
gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa... 121 4e-027
gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. v... 95 3e-025
gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. v... 77 1e-024
gb|BQ765947.1|BQ765947 EBro03_SQ008_I17_R root, 3 week, wat... 92 2e-024
gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. v... 95 5e-024
gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Li... 110 9e-024
gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgar... 89 2e-023
gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, wat... 106 1e-022
gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgar... 89 2e-022
>gb|BI950935.1|BI950935 HVSMEl0023L12f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023L12f, mRNA sequence
Length = 536
Score = 418 bits (908), Expect = e-117
Identities = 166/177 (93%), Positives = 172/177 (97%)
Frame = +1 / +2
Query: 49 LRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIVTRR 228
LRVQGDL+AF+NCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLI+TRR
Sbjct: 2 LRVQGDLSAFFNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVFQNCLIITRR 181
Query: 229 PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIPSYLGRPWKEFSRLVIM 408
PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLF DRFKIPSYLGRPWKEFSRLVIM
Sbjct: 182 PMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFVDRFKIPSYLGRPWKEFSRLVIM 361
Query: 409 ESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSKRVTWPGFHVIGRKDAE 579
ES IADFIKPEGYMPWNGDFG+KTLYYAEY NRGPGAGTSKRVTWPGF VI R++AE
Sbjct: 362 ESQIADFIKPEGYMPWNGDFGLKTLYYAEYGNRGPGAGTSKRVTWPGFRVIARQEAE 532
Score = 346 bits (750), Expect = 6e-095
Identities = 148/167 (88%), Positives = 150/167 (89%)
Frame = -1 / -3
Query: 581 CSASFRPMTWNPGQVTLLLVPAPGPRLLYSA**SVLMPKSPFQGM*PSGLMKSAMVLSMM 402
CSAS MT PGQVTLLLVPAPGPRL YSA**SVL PKSPF GM PSGLMKSA+ LSMM
Sbjct: 534 CSASCLAMTRKPGQVTLLLVPAPGPRLPYSA**SVLSPKSPFHGMYPSGLMKSAIWLSMM 355
Query: 401 TRRENSFQGRPRYEGILKRSGKSFWSGTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRR 222
T RENSF G PR +GILKRS KSFWSGTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRR
Sbjct: 354 TSRENSFHGLPR*DGILKRSTKSFWSGTRRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRR 175
Query: 221 VTMRQFWNTAAELPKMKSMVPEMTQLRKNWRRACTYSVSWNASNRQL 81
V MRQFWNTAAELPKMKSMVPEMTQLRKN RRACTYSVSWNASNRQL
Sbjct: 174 VMMRQFWNTAAELPKMKSMVPEMTQLRKNCRRACTYSVSWNASNRQL 34
Score = 189 bits (408), Expect(3) = 6e-059
Identities = 77/97 (79%), Positives = 83/97 (85%)
Frame = +3 / +1
Query: 198 VPELPHRHAAAHGQPAELGDRARPHGPQHEVGARHPELPPGARPEALPRPLQDPLVPGPT 377
VPELPH HAAAHGQPAELGDRAR HGPQHEV A HPELPPGA PEAL PLQ+P++PG
Sbjct: 151 VPELPHHHAAAHGQPAELGDRARAHGPQHEVRAGHPELPPGAGPEALRGPLQNPVLPGQA 330
Query: 378 LEGVLAPRHHGEHHRRLHQARGLHALERRLRHQDALL 488
+EGVLA HHGE RRLHQARG+HA+ERRLR QDALL
Sbjct: 331 VEGVLAAGHHGEPDRRLHQARGVHAVERRLRAQDALL 441
Score = 137 bits (293), Expect(4) = 3e-072
Identities = 63/99 (63%), Positives = 68/99 (68%)
Frame = -3 / -2
Query: 582 LLGVLPADDVEPRPGDPXXXXXXXXXXXXXXXVERLDAEVAVPGHVALGLDEVGDGALHD 403
+LG+LP DD E RPGDP VERL+ EVAVP HV LGLDEVGD ALHD
Sbjct: 535 MLGLLPGDDSEARPGDPLAGAGAGAAVAVLGVVERLEPEVAVPRHVPLGLDEVGDLALHD 356
Query: 402 DEARELLPGSAQVRGDLEAVGEELLVGHQAAVLDDEPRL 286
D+ RELLP AQV D EAV EELLV HQAAVLDD+P L
Sbjct: 355 DQPRELLPRPAQVGRDFEAVHEELLVRHQAAVLDDQPGL 239
Score = 87.7 bits (185), Expect(4) = 3e-072
Identities = 36/39 (92%), Positives = 37/39 (94%)
Frame = -3 / -2
Query: 228 PPRDDEAVLEHRRRVAEDEVNGAGDDAVAEELAPGVHVQ 112
PPRDDEAVLEH RRVAEDEV+GAGDDAVAEEL PGVHVQ
Sbjct: 181 PPRDDEAVLEHGRRVAEDEVDGAGDDAVAEELPPGVHVQ 65
Score = 59.7 bits (124), Expect(4) = 3e-072
Identities = 20/21 (95%), Positives = 20/21 (95%)
Frame = -2 / -1
Query: 283 CWGPCGRARSPSSAGCPWAAA 221
CWGPC RARSPSSAGCPWAAA
Sbjct: 236 CWGPCARARSPSSAGCPWAAA 174
Score = 47.8 bits (98), Expect(4) = 3e-072
Identities = 19/24 (79%), Positives = 20/24 (83%)
Frame = -2 / -1
Query: 136 TGAGRARTACPGTRRTGSCRTPRG 65
T AGRARTACPGTR+TGS R P G
Sbjct: 89 TAAGRARTACPGTRQTGS*RRPTG 18
Score = 47.8 bits (98), Expect(3) = 6e-059
Identities = 20/23 (86%), Positives = 21/23 (91%)
Frame = +3 / +1
Query: 75 VLQLPVRRVPGHAVRARPAPVLP 143
+LQLPV RVPGHAVRARPA VLP
Sbjct: 28 LLQLPV*RVPGHAVRARPAAVLP 96
Score = 28.6 bits (56), Expect(3) = 6e-059
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +3 / +1
Query: 531 EGHLAWVPRHRPEGRRA 581
EGHLA +P HR G RA
Sbjct: 484 EGHLAGLPSHRQAGGRA 534
>gb|BI780849.2|BI780849 EBma05_SQ001_C24_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ001_C24 5', mRNA sequence
Length = 602
Score = 400 bits (867), Expect = e-111
Identities = 159/166 (95%), Positives = 164/166 (98%)
Frame = +1 / +3
Query: 1 MGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIF 180
MGFHNTAGAE+HQAVALRVQGDL+AF+NCRFDAFQDTLYVHARRQFFRNCVISGTIDFIF
Sbjct: 105 MGFHNTAGAEKHQAVALRVQGDLSAFFNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIF 284
Query: 181 GNSAAVFQNCLIVTRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFPDRFKIP 360
GNSAAVFQNCLI+TRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLF DRFKIP
Sbjct: 285 GNSAAVFQNCLIITRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRLVPDQKLFVDRFKIP 464
Query: 361 SYLGRPWKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEY 498
SYLGRPWKEFSRLVIMES IADFIKPEGYMPWNGDFG+KTLYYAEY
Sbjct: 465 SYLGRPWKEFSRLVIMESQIADFIKPEGYMPWNGDFGLKTLYYAEY 602
Score = 324 bits (701), Expect = 3e-088
Identities = 137/165 (83%), Positives = 139/165 (84%)
Frame = -1 / -2
Query: 497 YSA**SVLMPKSPFQGM*PSGLMKSAMVLSMMTRRENSFQGRPRYEGILKRSGKSFWSGT 318
YSA**SVL PKSPF GM PSGLMKSA+ LSMMT RENSF G PR +GILKRS KSFWSGT
Sbjct: 601 YSA**SVLSPKSPFHGMYPSGLMKSAIWLSMMTSRENSFHGLPR*DGILKRSTKSFWSGT 422
Query: 317 RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVTMRQFWNTAAELPKMKSMVPEMTQLRK 138
RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRV MRQFWNTAAELPKMKSMVPEMTQLRK
Sbjct: 421 RRQFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVMMRQFWNTAAELPKMKSMVPEMTQLRK 242
Query: 137 NWRRACTYSVSWNASNRQL*NXXXXXXXXXXXAWCLSAPAVLWKP 3
N RRACTYSVSWNASNRQL AWC SAPAVLW P
Sbjct: 241 NCRRACTYSVSWNASNRQLKKADRSPCTLSATAWCFSAPAVLWNP 107
Score = 189 bits (408), Expect(2) = 5e-056
Identities = 77/97 (79%), Positives = 83/97 (85%)
Frame = +3 / +2
Query: 198 VPELPHRHAAAHGQPAELGDRARPHGPQHEVGARHPELPPGARPEALPRPLQDPLVPGPT 377
VPELPH HAAAHGQPAELGDRAR HGPQHEV A HPELPPGA PEAL PLQ+P++PG
Sbjct: 302 VPELPHHHAAAHGQPAELGDRARAHGPQHEVRAGHPELPPGAGPEALRGPLQNPVLPGQA 481
Query: 378 LEGVLAPRHHGEHHRRLHQARGLHALERRLRHQDALL 488
+EGVLA HHGE RRLHQARG+HA+ERRLR QDALL
Sbjct: 482 VEGVLAAGHHGEPDRRLHQARGVHAVERRLRAQDALL 592
Score = 110 bits (234), Expect(5) = 1e-068
Identities = 50/66 (75%), Positives = 53/66 (80%)
Frame = -3 / -1
Query: 483 ERLDAEVAVPGHVALGLDEVGDGALHDDEARELLPGSAQVRGDLEAVGEELLVGHQAAVL 304
ERL+ EVAVP HV LGLDEVGD ALHDD+ RELLP AQV D EAV EELLV HQAAVL
Sbjct: 587 ERLEPEVAVPRHVPLGLDEVGDLALHDDQPRELLPRPAQVGRDFEAVHEELLVRHQAAVL 408
Query: 303 DDEPRL 286
DD+P L
Sbjct: 407 DDQPGL 390
Score = 87.7 bits (185), Expect(5) = 1e-068
Identities = 36/39 (92%), Positives = 37/39 (94%)
Frame = -3 / -1
Query: 228 PPRDDEAVLEHRRRVAEDEVNGAGDDAVAEELAPGVHVQ 112
PPRDDEAVLEH RRVAEDEV+GAGDDAVAEEL PGVHVQ
Sbjct: 332 PPRDDEAVLEHGRRVAEDEVDGAGDDAVAEELPPGVHVQ 216
Score = 59.7 bits (124), Expect(5) = 1e-068
Identities = 20/21 (95%), Positives = 20/21 (95%)
Frame = -2 / -3
Query: 283 CWGPCGRARSPSSAGCPWAAA 221
CWGPC RARSPSSAGCPWAAA
Sbjct: 387 CWGPCARARSPSSAGCPWAAA 325
Score = 47.8 bits (98), Expect(5) = 1e-068
Identities = 19/24 (79%), Positives = 20/24 (83%)
Frame = -2 / -3
Query: 136 TGAGRARTACPGTRRTGSCRTPRG 65
T AGRARTACPGTR+TGS R P G
Sbjct: 240 TAAGRARTACPGTRQTGS*RRPTG 169
Score = 47.8 bits (98), Expect(2) = 5e-056
Identities = 20/23 (86%), Positives = 21/23 (91%)
Frame = +3 / +2
Query: 75 VLQLPVRRVPGHAVRARPAPVLP 143
+LQLPV RVPGHAVRARPA VLP
Sbjct: 179 LLQLPV*RVPGHAVRARPAAVLP 247
Score = 32.7 bits (65), Expect(5) = 1e-068
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = -3 / -1
Query: 63 SLNAERHRLVPLRAGRVVEAH 1
+L+ +RHRLV LRA RVVE H
Sbjct: 167 ALHPQRHRLVFLRARRVVEPH 105
>gb|BJ465418.1|BJ465418 BJ465418 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39m02 5', mRNA sequence
Length = 528
Score = 162 bits (349), Expect = 1e-039
Identities = 64/102 (62%), Positives = 76/102 (74%)
Frame = +1 / +1
Query: 13 NTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSA 192
N AG +HQAVALRV DL+AFY C F +QDTLYVH+ RQFFR C I GTIDF+FGNSA
Sbjct: 217 NAAGPSKHQAVALRVGADLSAFYRCSFVGYQDTLYVHSLRQFFRECDIYGTIDFVFGNSA 396
Query: 193 AVFQNCLIVTRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 318
AV Q+C + RRP+ NQ N TA GRTDPN +G+ IQ C++
Sbjct: 397 AVLQSCNLYARRPLPNQSNIYTAQGRTDPNQNTGISIQKCKV 522
Score = 112 bits (239), Expect = 2e-024
Identities = 51/97 (52%), Positives = 54/97 (55%)
Frame = -1 / -2
Query: 308 FWMTSPDFMLGSVRPCAVTEFCWLSMGRRVTMRQFWNTAAELPKMKSMVPEMTQLRKNWR 129
FWM P F LGSVRPCAV WL GRR Q W+TAAELPK KSMVP M+ RKN R
Sbjct: 512 FWMEMPVFWLGSVRPCAV*MLLWLGRGRRA*RLQLWSTAAELPKTKSMVP*MSHSRKN*R 333
Query: 128 RACTYSVSWNASNRQL*NXXXXXXXXXXXAWCLSAPA 18
R CT VSW + * AWCL PA
Sbjct: 332 RECT*RVSW*PTKLHR*KAERSAPTRSATAWCLDGPA 222
>gb|BQ754059.1|BQ754059 EBca01_SQ002_L07_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_L07 5', mRNA sequence
Length = 372
Score = 158 bits (339), Expect = 3e-038
Identities = 60/106 (56%), Positives = 78/106 (73%)
Frame = +1 / +2
Query: 1 MGFHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIF 180
M F NTAG +HQAVALR DL+ FY C F+ QDTLY H+ RQF+R+C ++GT+DF+F
Sbjct: 11 MTFENTAGPAKHQAVALRCDSDLSVFYRCAFEGHQDTLYAHSLRQFYRDCRVAGTVDFVF 190
Query: 181 GNSAAVFQNCLIVTRRPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 318
GN+AAVFQNCL++ R P+ Q+NSVTA GR + +M SG Q C +
Sbjct: 191 GNAAAVFQNCLLLARAPLPGQKNSVTAQGRFNASMNSGFRFQFCNV 328
Score = 114 bits (243), Expect = 5e-025
Identities = 50/99 (50%), Positives = 52/99 (52%)
Frame = -1 / -1
Query: 311 QFWMTSPDFMLGSVRPCAVTEFCWLSMGRRVTMRQFWNTAAELPKMKSMVPEMTQLRKNW 132
Q W P+FM RP AVTEF W G R RQFWNTAA LPK KS VP Q R NW
Sbjct: 321 QNWKRKPEFMEALKRPWAVTEFFWPGSGARARRRQFWNTAAALPKTKSTVPATRQSR*NW 142
Query: 131 RRACTYSVSWNASNRQL*NXXXXXXXXXXXAWCLSAPAV 15
R C YSVSW S * AWCL+ PAV
Sbjct: 141 RSECAYSVSWCPSKAHR*KTERSESQRSATAWCLAGPAV 25
>gb|BI956815.1|BI956815 HVSMEn0005H07f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005H07f, mRNA sequence
Length = 877
Score = 98.2 bits (208), Expect(3) = 1e-034
Identities = 36/71 (50%), Positives = 50/71 (70%)
Frame = +1 / +3
Query: 124 ARRQFFRNCVISGTIDFIFGNSAAVFQNCLIVTRRPMDNQQNSVTAHGRTDPNMKSGLVI 303
+ RQF+R CVISGT+DFI+GN+ AVFQ+CL++ R P+ + N +TA GR P+ SG
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 304 QNCRLVPDQKL 336
QNCR+ + L
Sbjct: 186 QNCRVTSQEDL 218
Score = 45.1 bits (92), Expect(3) = 1e-034
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +1 / +3
Query: 361 SYLGRPWKEFSRLVIMESTIADFIKPEGYMPWN 459
+Y GRPWK S +V M+S + + P G++ WN
Sbjct: 237 TYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAWN 335
Score = 40.9 bits (83), Expect(3) = 1e-034
Identities = 14/22 (63%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 478 TLYYAEYNNRGPGAGTSKRVTW 543
T++Y EY N GPGA SKRV W
Sbjct: 360 TVFYGEYGNTGPGANLSKRVPW 425
>gb|BI956859.1|BI956859 HVSMEn0005L01f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005L01f, mRNA sequence
Length = 867
Score = 98.2 bits (208), Expect(3) = 3e-034
Identities = 36/71 (50%), Positives = 50/71 (70%)
Frame = +1 / +3
Query: 124 ARRQFFRNCVISGTIDFIFGNSAAVFQNCLIVTRRPMDNQQNSVTAHGRTDPNMKSGLVI 303
+ RQF+R CVISGT+DFI+GN+ AVFQ+CL++ R P+ + N +TA GR P+ SG
Sbjct: 6 SNRQFYRECVISGTVDFIYGNAKAVFQDCLLLARAPLKGKHNVITAQGRDGPDGSSGFSF 185
Query: 304 QNCRLVPDQKL 336
QNCR+ + L
Sbjct: 186 QNCRVTSQEDL 218
Score = 45.1 bits (92), Expect(3) = 3e-034
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = +1 / +3
Query: 361 SYLGRPWKEFSRLVIMESTIADFIKPEGYMPWN 459
+Y GRPWK S +V M+S + + P G++ WN
Sbjct: 237 TYFGRPWKNHSHVVFMQSFLDTIVHPSGWVAWN 335
Score = 39.6 bits (80), Expect(3) = 3e-034
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = +1 / +3
Query: 478 TLYYAEYNNRGPGAGTSKRVTW 543
T++Y EY N GPGA +KRV W
Sbjct: 360 TVFYGEYGNTGPGANLTKRVPW 425
>gb|BG415761.2|BG415761 HVSMEk0007L15f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0007L15f, mRNA sequence
Length = 650
Score = 121 bits (258), Expect = 4e-027
Identities = 47/90 (52%), Positives = 55/90 (61%)
Frame = +1 / +2
Query: 13 NTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSA 192
NTA E H AVALRV D A CR D +QDTLY H R F+R+C + GT+DF F N+
Sbjct: 368 NTAVPEMHHAVALRVSADRAVINRCRVDGYQDTLYAHQLRDFYRDCAVCGTVDFFFSNAG 547
Query: 193 AVFQNCLIVTRRPMDNQQNSVTAHGRTDPN 282
V Q CL+ RR Q+ VT HG TDPN
Sbjct: 548 VVLQGCLLTERRAARGQKKRVTGHGMTDPN 637
>gb|CB859122.1|CB859122 HI09G17w HI Hordeum vulgare subsp. vulgare cDNA clone HI09G17
3-PRIME, mRNA sequence
Length = 565
Score = 95.5 bits (202), Expect(2) = 3e-025
Identities = 32/56 (57%), Positives = 44/56 (78%)
Frame = +1 / -1
Query: 364 YLGRPWKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSK 531
+LGRPWKE+SR V + T+A+ ++P G+MPW+GDF + TLYY EY + GPGAG S+
Sbjct: 376 FLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRSR 209
Score = 39.1 bits (79), Expect(2) = 3e-025
Identities = 13/31 (41%), Positives = 22/31 (70%)
Frame = +1 / -1
Query: 226 RPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 318
RP + +++TA GRTDP +G+V++ CR+
Sbjct: 526 RPEKGETDALTAQGRTDPAQPTGIVLRGCRV 434
>gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. vulgare cDNA clone HX05G15
5-PRIME, mRNA sequence
Length = 638
Score = 76.7 bits (161), Expect(2) = 1e-024
Identities = 28/63 (44%), Positives = 40/63 (63%)
Frame = +1 / +1
Query: 22 GAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSAAVF 201
G VALR+ D AAF C F QDTLY H R ++++C I G++DFIFGN+ +++
Sbjct: 4 GRAGEAGVALRISADNAAFVGCNFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLY 183
Query: 202 QNC 210
+ C
Sbjct: 184 EGC 192
Score = 57.4 bits (119), Expect(2) = 1e-024
Identities = 23/60 (38%), Positives = 32/60 (53%)
Frame = +1 / +1
Query: 364 YLGRPWKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSKRVTW 543
YLGR W FSR+V + + + I P G+ W T++Y +Y GPGA + RV W
Sbjct: 310 YLGRAWGTFSRVVFAYTYMDNIIIPRGWYNWGDPTREMTVFYGQYKCSGPGANHAGRVDW 489
>gb|BQ765947.1|BQ765947 EBro03_SQ008_I17_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_I17 5', mRNA sequence
Length = 723
Score = 91.8 bits (194), Expect(3) = 2e-024
Identities = 37/70 (52%), Positives = 48/70 (68%)
Frame = +1 / +3
Query: 7 FHNTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGN 186
F N+A QAVA+RV D AFY+CRF +QDTLY+H +Q+ R+C I G DFIFGN
Sbjct: 45 FQNSAPQGSGQAVAVRVTADKCAFYSCRFLGWQDTLYLHYGKQYLRDCYIEGNCDFIFGN 224
Query: 187 SAAVFQNCLI 216
S A+ ++C I
Sbjct: 225 SIALLEHCHI 254
Score = 38.2 bits (77), Expect(3) = 2e-024
Identities = 16/50 (32%), Positives = 25/50 (50%)
Frame = +1 / +1
Query: 394 RLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSKRVTW 543
R+V + + +K G+ W+ +T + EY GPG+ TS RV W
Sbjct: 400 RVVFAHTFMDRCVKATGWHNWDKSENERTACFYEYRCSGPGSRTSSRVAW 549
Score = 21.7 bits (41), Expect(3) = 2e-024
Identities = 6/11 (54%), Positives = 8/11 (72%)
Frame = +1 / +3
Query: 364 YLGRPWKEFSR 396
+LGRPW F +
Sbjct: 369 FLGRPWGPFGK 401
>gb|CB859989.1|CB859989 HI12E03w HI Hordeum vulgare subsp. vulgare cDNA clone HI12E03
3-PRIME, mRNA sequence
Length = 521
Score = 95.5 bits (202), Expect(2) = 5e-024
Identities = 32/56 (57%), Positives = 44/56 (78%)
Frame = +1 / -1
Query: 364 YLGRPWKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSK 531
+LGRPWKE+SR V + T+A+ ++P G+MPW+GDF + TLYY EY + GPGAG S+
Sbjct: 377 FLGRPWKEYSRTVFVGCTLAEIVQPAGWMPWSGDFALDTLYYGEYGSAGPGAGRSR 210
Score = 35.0 bits (70), Expect(2) = 5e-024
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = +1 / -1
Query: 241 QQNSVTAHGRTDPNMKSGLVIQNCRL 318
+ +++TA GRTDP +G+V++ CR+
Sbjct: 512 ETDALTAQGRTDPAQPTGIVLRGCRV 435
>gb|BE422260.1|BE422260 HWM022cC.01r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM022cC.01, mRNA sequence
Length = 1239
Score = 110 bits (234), Expect = 9e-024
Identities = 39/67 (58%), Positives = 49/67 (73%)
Frame = +1 / +3
Query: 361 SYLGRPWKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSKRVT 540
SYLGRPWK++SR V M+S + + P G++ W+G F + TLYY EY N GPGAGTS RVT
Sbjct: 117 SYLGRPWKQYSRTVFMQSELDSVVNPAGWLAWDGTFALDTLYYGEYQNTGPGAGTSGRVT 296
Query: 541 WPGFHVI 561
W G+ VI
Sbjct: 297 WKGYRVI 317
>gb|CK122985.1|CK122985 BES1824101k13 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K131 5-PRIME, mRNA sequence
Length = 611
Score = 89.0 bits (188), Expect(2) = 2e-023
Identities = 36/69 (52%), Positives = 44/69 (63%)
Frame = +1 / +1
Query: 13 NTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSA 192
NTAG + HQAVA R GD QDTLY HA RQF+ C+++GT+DFIFGNSA
Sbjct: 217 NTAGPDAHQAVAFRSTGDRTVLDTVELAGHQDTLYAHAMRQFYTRCLVAGTVDFIFGNSA 396
Query: 193 AVFQNCLIV 219
AV + +V
Sbjct: 397 AVLHDSDVV 423
Score = 39.1 bits (79), Expect(2) = 2e-023
Identities = 13/31 (41%), Positives = 22/31 (70%)
Frame = +1 / +1
Query: 226 RPMDNQQNSVTAHGRTDPNMKSGLVIQNCRL 318
RP + +++TA GRTDP +G+V++ CR+
Sbjct: 442 RPEXGETDALTAQGRTDPAQPTGIVLRGCRV 534
>gb|BQ765469.1|BQ765469 EBro03_SQ007_C09_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ007_C09 5', mRNA sequence
Length = 417
Score = 106 bits (226), Expect = 1e-022
Identities = 36/61 (59%), Positives = 48/61 (78%)
Frame = +1 / +2
Query: 379 WKEFSRLVIMESTIADFIKPEGYMPWNGDFGIKTLYYAEYNNRGPGAGTSKRVTWPGFHV 558
WK +SR VIM+S +A + P G+MPW+GDF + TLYYAEY+N G G+ TS+RV WPG+HV
Sbjct: 2 WKMYSRTVIMQSDVAGIVDPAGWMPWSGDFALSTLYYAEYDNSGAGSDTSRRVNWPGYHV 181
Query: 559 I 561
+
Sbjct: 182 L 184
>gb|CK122193.1|CK122193 BES1824101p15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P151 5-PRIME, mRNA sequence
Length = 641
Score = 89.0 bits (188), Expect(3) = 2e-022
Identities = 36/69 (52%), Positives = 44/69 (63%)
Frame = +1 / +1
Query: 13 NTAGAERHQAVALRVQGDLAAFYNCRFDAFQDTLYVHARRQFFRNCVISGTIDFIFGNSA 192
NTAG + HQAVA R GD QDTLY HA RQF+ C+++GT+DFIFGNSA
Sbjct: 217 NTAGPDAHQAVAFRSTGDRTVLDTVELAGHQDTLYAHAMRQFYTRCLVAGTVDFIFGNSA 396
Query: 193 AVFQNCLIV 219
AV + +V
Sbjct: 397 AVLHDSDVV 423
Score = 29.9 bits (59), Expect(3) = 2e-022
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +1 / +2
Query: 364 YLGRPWKEFSRLV 402
+LGRPWKE+SR V
Sbjct: 593 FLGRPWKEYSRPV 631
Score = 23.5 bits (45), Expect(3) = 2e-022
Identities = 14/41 (34%), Positives = 15/41 (36%)
Frame = +3 / +3
Query: 201 PELPHRHAAAHGQPAELGDRARPHGPQHEVGARHPELPPGA 323
P R AAA E D H GA H + PGA
Sbjct: 405 PRQRRRGAAAAAAAGEGRDGRAHGAGPHRPGAAHGDRAPGA 527
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 228,025,237
Number of Sequences: 312970
Number of extensions: 3949602
Number of successful extensions: 97421
Number of sequences better than 1.0e-020: 21
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6192839514
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)