TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3197099.2.1
(631 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV921670.1|AV921670 AV921670 K. Sato unpublished cDNA li... 175 3e-051
gb|CB874756.1|CB874756 HX06D16w HX Hordeum vulgare subsp. v... 149 2e-043
gb|BG415995.2|BG415995 HVSMEk0009F13f Hordeum vulgare testa... 149 8e-043
gb|BJ468650.1|BJ468650 BJ468650 K. Sato unpublished cDNA li... 149 1e-042
gb|CB874722.1|CB874722 HX06C02w HX Hordeum vulgare subsp. v... 149 1e-042
gb|CA030113.1|CA030113 HX06C02r HX Hordeum vulgare subsp. v... 149 1e-042
gb|BJ473415.1|BJ473415 BJ473415 K. Sato unpublished cDNA li... 149 1e-042
gb|AV916245.1|AV916245 AV916245 K. Sato unpublished cDNA li... 152 1e-036
gb|BJ451080.1|BJ451080 BJ451080 K. Sato unpublished cDNA li... 144 4e-034
gb|CB875233.1|CB875233 HX07K22w HX Hordeum vulgare subsp. v... 116 2e-033
gb|BU973725.1|BU973725 HB25M17r BC Hordeum vulgare subsp. v... 127 4e-033
gb|BM100617.2|BM100617 EBma01_SQ005_L23_R maternal, 4 DPA, ... 130 8e-030
gb|BU996805.1|BU996805 HM14N04r HM Hordeum vulgare subsp. v... 126 1e-028
gb|BU971142.1|BU971142 HB16L19r BC Hordeum vulgare subsp. v... 124 5e-028
gb|BE215344.1|BE215344 HV_CEb0006I15f Hordeum vulgare seedl... 120 6e-027
gb|BJ457634.1|BJ457634 BJ457634 K. Sato unpublished cDNA li... 90 1e-022
gb|BE195976.3|BE195976 HVSMEh0091A07f Hordeum vulgare 5-45 ... 95 6e-022
gb|BU975642.1|BU975642 HB31N05r BC Hordeum vulgare subsp. v... 95 1e-021
>gb|AV921670.1|AV921670 AV921670 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags12e13 3', mRNA sequence
Length = 589
Score = 175 bits (377), Expect(2) = 6e-050
Identities = 77/101 (76%), Positives = 82/101 (81%)
Frame = +3 / +2
Query: 282 CQQ*NIILFTFSFTAGRGFTETPLGPLEVLDVWLSNISGSMXXXXXXXXKCA*YAGGTTQ 461
CQQ*NI LFTFSFTAG+GFTE P PLE+ DVW S+ SGSM +CA*YAGGTT+
Sbjct: 203 CQQ*NITLFTFSFTAGKGFTEVPFVPLELFDVWFSDSSGSM*NRARNAARCA*YAGGTTE 382
Query: 462 TERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE*AGLLVPW 584
TERVHRA*V*QRLF N CISSA KLFSSQRTW *AGLLVPW
Sbjct: 383 TERVHRA*V*QRLFVNVCISSAEKLFSSQRTW**AGLLVPW 505
Score = 171 bits (369), Expect(2) = 3e-051
Identities = 71/101 (70%), Positives = 78/101 (77%)
Frame = -1 / -2
Query: 583 QGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXXXXXXXXXXXXXXXRFY 404
QGTSRPAH HVLWDENNF+ADEMQ L NNLCYTYARCTRSV RFY
Sbjct: 504 QGTSRPAHYHVLWDENNFSADEMQTLTNNLCYTYARCTRSVSVVPPAYYAHLAAFRARFY 325
Query: 403 MEPEMLDNQTSKTSNGPSGVSVKPLPAVKEKVKRMMFYC*Q 281
MEPE+ +N TSK+S+G +G SVKPLPAVKEKVKR+MFYC*Q
Sbjct: 324 MEPELSENHTSKSSSGTNGTSVKPLPAVKEKVKRVMFYC*Q 202
Score = 127 bits (272), Expect(2) = 9e-035
Identities = 49/78 (62%), Positives = 56/78 (71%)
Frame = +1 / +3
Query: 352 SGHWRSWTSGCPTSLVPCRTEPAMLPSVRSMQEGQHKPSVCTGHKCSKGCLAMAAFRLL* 531
S HW S T G PT+ PCRT P M P R+MQ G+ K S CT KCSKGCL+ +AFRLL
Sbjct: 273 SSHWSSSTCGSPTAPAPCRTAPGMQPDARNMQVGRRKQSACTVRKCSKGCLSTSAFRLLR 452
Query: 532 NCSHPRGHGSEQAYSFPG 585
+CSHPRGHGSE AY +PG
Sbjct: 453 SCSHPRGHGSELAYLYPG 506
Score = 120 bits (257), Expect(2) = 1e-032
Identities = 58/110 (52%), Positives = 69/110 (62%)
Frame = -3 / -1
Query: 584 PGNE*ACSLPCPLG*EQFHSRRNAAIAKQPLLHLCPVHTLGLCCPSCILRTLGSIAGSVL 405
PG +*A SLPCPLG*EQ SRRNA + KQPLLHL VH L P+CILR G I G+VL
Sbjct: 505 PGYK*ASSLPCPLG*EQLLSRRNADVDKQPLLHLRTVHALCFRRPTCILRASGCIPGAVL 326
Query: 404 HGTRDVGQPDVQDLQWPERSLXXXXXXXXXXXEKDDVLLLTRSPLNHSHA 255
HG VG+P V++LQW E L E+ DVLLLT + +H+
Sbjct: 325 HGAGAVGEPHVEELQWDEWYLREAFARCEREGEEGDVLLLTEGLVLTTHS 176
Score = 119 bits (255), Expect(2) = 5e-032
Identities = 56/98 (57%), Positives = 64/98 (65%)
Frame = +2 / +1
Query: 290 VEHHPFHLLLHSRQGLHRDSARAIGGLGRLVVQHLWFHVEPSPXXXXXXXXXRRDNTNRA 469
VEHHP HLL HS Q LH + R G L R+V++ L HVEP P R D+ NRA
Sbjct: 211 VEHHPLHLLFHSGQRLHGGTIRPTGALRRVVLRQLRLHVEPRPECSQMRVICRWDDGNRA 390
Query: 470 CAPGISVAKVVWQWLHFVCCEIVLIPEDMGVSRPTRSL 583
AP +SVAKVV Q LHFVC E+VLIPEDM VS PT +L
Sbjct: 391 RAPCVSVAKVVCQRLHFVC*EVVLIPEDMVVSWPTCTL 504
Score = 94.1 bits (199), Expect(2) = 6e-023
Identities = 42/68 (61%), Positives = 45/68 (66%)
Frame = -2 / -3
Query: 582 RERVGLLTPMSSGMRTISQQTKCSHCQTTFATLMPGAHARFVLSLLHTTHTWQHCGLGST 403
R +VG LT MSSGMRT SQQTKC QTTFATL GA A F S LH T W H G GST
Sbjct: 503 RVQVGQLTTMSSGMRTTSQQTKCRR*QTTFATLTHGARALFPSSHLHITRIWLHSGRGST 324
Query: 402 WNQRCWTT 379
W++ C T
Sbjct: 323 WSRSCRRT 300
Score = 50.1 bits (103), Expect(2) = 3e-051
Identities = 17/18 (94%), Positives = 17/18 (94%)
Frame = -1 / -1
Query: 631 CHPTEFDFYLCSHAGIQG 578
CHPTEFDFYLCSHAGI G
Sbjct: 553 CHPTEFDFYLCSHAGIPG 500
Score = 41.8 bits (85), Expect(2) = 6e-050
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = +1 / +1
Query: 583 GFQHDYRGRNQTPLGG 630
GFQ DYRGRNQTPLGG
Sbjct: 505 GFQRDYRGRNQTPLGG 552
Score = 39.1 bits (79), Expect(2) = 9e-035
Identities = 17/19 (89%), Positives = 17/19 (89%)
Frame = +3 / +3
Query: 573 LVPWIPA*LQR*KSNSVGW 629
L P IPA*LQR*KSNSVGW
Sbjct: 495 LYPGIPA*LQR*KSNSVGW 551
Score = 38.6 bits (78), Expect(2) = 1e-032
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = -3 / -3
Query: 629 PPNGV*FLPL*SCWNP 582
PPNGV*FLPL*S WNP
Sbjct: 551 PPNGV*FLPL*SRWNP 504
Score = 37.7 bits (76), Expect(2) = 5e-032
Identities = 15/16 (93%), Positives = 16/16 (100%)
Frame = +2 / +2
Query: 584 DSSMTTEVEIKLRWVA 631
DSS+TTEVEIKLRWVA
Sbjct: 506 DSSVTTEVEIKLRWVA 553
Score = 32.7 bits (65), Expect(2) = 6e-023
Identities = 15/17 (88%), Positives = 16/17 (94%)
Frame = -2 / -2
Query: 630 ATQRSLISTSVVMLESR 580
ATQRSLISTSVV LES+
Sbjct: 552 ATQRSLISTSVVTLESQ 502
>gb|CB874756.1|CB874756 HX06D16w HX Hordeum vulgare subsp. vulgare cDNA clone HX06D16
3-PRIME, mRNA sequence
Length = 635
Score = 149 bits (321), Expect(2) = 2e-043
Identities = 59/84 (70%), Positives = 62/84 (73%)
Frame = -1 / -3
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDEN FTADE+Q L NNLCYTYARCTRSV
Sbjct: 567 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIV 388
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDN 380
RFYMEP+ D+
Sbjct: 387 PPAYYAHLAAFRARFYMEPDTSDS 316
Score = 101 bits (215), Expect(2) = 3e-025
Identities = 52/83 (62%), Positives = 56/83 (67%)
Frame = +3 / +2
Query: 381 LSNISGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE 560
LS +SGSM KCA*YAGGT TERVH A V* +LF C SSAV LFSS T
Sbjct: 317 LSEVSGSM*NRARKAAKCA*YAGGTIDTERVHLAYV*HKLFVRVCNSSAVNLFSSHST** 496
Query: 561 *AGLLVPWIPA*LQR*KSNSVGW 629
*AG LVP +PA L +*KSNSVGW
Sbjct: 497 *AGRLVP*MPAWLHK*KSNSVGW 565
Score = 45.5 bits (93), Expect(2) = 2e-043
Identities = 18/34 (52%), Positives = 24/34 (70%)
Frame = -1 / -3
Query: 379 QTSKTSNGPSGVSVKPLPAVKEKVKRMMFYC*QG 278
Q + + V+V+PLPA+KE VKR+MFYC* G
Sbjct: 282 QGGRNNRAFGNVAVRPLPALKENVKRVMFYC*GG 181
Score = 33.1 bits (66), Expect(2) = 3e-025
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +3 / +2
Query: 288 Q*NIILFTFSFTAGRGFTET 347
Q*N+ FTFSF AGRG T T
Sbjct: 191 Q*NMTRFTFSFRAGRGLTAT 250
>gb|BG415995.2|BG415995 HVSMEk0009F13f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0009F13f, mRNA sequence
Length = 970
Score = 149 bits (321), Expect(2) = 8e-043
Identities = 59/84 (70%), Positives = 62/84 (73%)
Frame = -1 / +1
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDEN FTADE+Q L NNLCYTYARCTRSV
Sbjct: 124 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIV 303
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDN 380
RFYMEP+ D+
Sbjct: 304 PPAYYAHLAAFRARFYMEPDTSDS 375
Score = 102 bits (217), Expect(2) = 7e-024
Identities = 53/83 (63%), Positives = 57/83 (68%)
Frame = +3 / -3
Query: 381 LSNISGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE 560
LS +SGSM + A*YAGGT TERVHRA V* RLF C SSAV LFSSQRT
Sbjct: 374 LSEVSGSM*NRARKAARWA*YAGGTIDTERVHRAYV*HRLFVRVCNSSAVNLFSSQRT** 195
Query: 561 *AGLLVPWIPA*LQR*KSNSVGW 629
AG LVP +PA L +*KSNSVGW
Sbjct: 194 WAGRLVP*MPAWLHK*KSNSVGW 126
Score = 43.7 bits (89), Expect(2) = 8e-043
Identities = 17/25 (68%), Positives = 21/25 (84%)
Frame = -1 / +2
Query: 358 GPSGVSVKPLPAVKEKVKRMMFYC* 284
G V+V+PLPA+KE VKR+MFYC*
Sbjct: 437 GFGNVAVRPLPALKENVKRVMFYC* 511
Score = 27.6 bits (54), Expect(2) = 7e-024
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / -2
Query: 288 Q*NIILFTFSFTAGRGFTET 347
Q*N+ TFS AGRG T T
Sbjct: 507 Q*NMTRLTFSLRAGRGLTAT 448
>gb|BJ468650.1|BJ468650 BJ468650 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags39l05 3', mRNA sequence
Length = 673
Score = 149 bits (321), Expect(2) = 1e-042
Identities = 59/84 (70%), Positives = 62/84 (73%)
Frame = -1 / -3
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDEN FTADE+Q L NNLCYTYARCTRSV
Sbjct: 671 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIV 492
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDN 380
RFYMEP+ D+
Sbjct: 491 PPAYYAHLAAFRARFYMEPDTSDS 420
Score = 102 bits (217), Expect(2) = 7e-024
Identities = 53/83 (63%), Positives = 57/83 (68%)
Frame = +3 / +1
Query: 381 LSNISGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE 560
LS +SGSM + A*YAGGT TERVHRA V* RLF C SSAV LFSSQRT
Sbjct: 421 LSEVSGSM*NRARKAARWA*YAGGTIDTERVHRAYV*HRLFVRVCNSSAVNLFSSQRT** 600
Query: 561 *AGLLVPWIPA*LQR*KSNSVGW 629
AG LVP +PA L +*KSNSVGW
Sbjct: 601 WAGRLVP*MPAWLHK*KSNSVGW 669
Score = 43.2 bits (88), Expect(2) = 1e-042
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = -1 / -3
Query: 370 KTSNGPSGVSVKPLPAVKEKVKRMMFYC* 284
++S V+V+PLPA+KE VKR+MFYC*
Sbjct: 371 RSSTRFGNVAVRPLPALKENVKRVMFYC* 285
Score = 27.6 bits (54), Expect(2) = 7e-024
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / +1
Query: 288 Q*NIILFTFSFTAGRGFTET 347
Q*N+ TFS AGRG T T
Sbjct: 289 Q*NMTRLTFSLRAGRGLTAT 348
>gb|CB874722.1|CB874722 HX06C02w HX Hordeum vulgare subsp. vulgare cDNA clone HX06C02
3-PRIME, mRNA sequence
Length = 645
Score = 149 bits (321), Expect(2) = 1e-042
Identities = 59/84 (70%), Positives = 62/84 (73%)
Frame = -1 / -2
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDEN FTADE+Q L NNLCYTYARCTRSV
Sbjct: 572 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIV 393
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDN 380
RFYMEP+ D+
Sbjct: 392 PPAYYAHLAAFRARFYMEPDTSDS 321
Score = 102 bits (217), Expect(2) = 7e-024
Identities = 53/83 (63%), Positives = 57/83 (68%)
Frame = +3 / +1
Query: 381 LSNISGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE 560
LS +SGSM + A*YAGGT TERVHRA V* RLF C SSAV LFSSQRT
Sbjct: 322 LSEVSGSM*NRARKAARWA*YAGGTIDTERVHRAYV*HRLFVRVCNSSAVNLFSSQRT** 501
Query: 561 *AGLLVPWIPA*LQR*KSNSVGW 629
AG LVP +PA L +*KSNSVGW
Sbjct: 502 WAGRLVP*MPAWLHK*KSNSVGW 570
Score = 43.2 bits (88), Expect(2) = 1e-042
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = -1 / -2
Query: 370 KTSNGPSGVSVKPLPAVKEKVKRMMFYC* 284
++S V+V+PLPA+KE VKR+MFYC*
Sbjct: 272 RSSTRFGNVAVRPLPALKENVKRVMFYC* 186
Score = 27.6 bits (54), Expect(2) = 7e-024
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / +1
Query: 288 Q*NIILFTFSFTAGRGFTET 347
Q*N+ TFS AGRG T T
Sbjct: 190 Q*NMTRLTFSLRAGRGLTAT 249
>gb|CA030113.1|CA030113 HX06C02r HX Hordeum vulgare subsp. vulgare cDNA clone HX06C02
5-PRIME, mRNA sequence
Length = 624
Score = 149 bits (321), Expect(2) = 1e-042
Identities = 59/84 (70%), Positives = 62/84 (73%)
Frame = -1 / +3
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDEN FTADE+Q L NNLCYTYARCTRSV
Sbjct: 237 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIV 416
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDN 380
RFYMEP+ D+
Sbjct: 417 PPAYYAHLAAFRARFYMEPDTSDS 488
Score = 102 bits (217), Expect(2) = 7e-024
Identities = 53/83 (63%), Positives = 57/83 (68%)
Frame = +3 / -3
Query: 381 LSNISGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE 560
LS +SGSM + A*YAGGT TERVHRA V* RLF C SSAV LFSSQRT
Sbjct: 487 LSEVSGSM*NRARKAARWA*YAGGTIDTERVHRAYV*HRLFVRVCNSSAVNLFSSQRT** 308
Query: 561 *AGLLVPWIPA*LQR*KSNSVGW 629
AG LVP +PA L +*KSNSVGW
Sbjct: 307 WAGRLVP*MPAWLHK*KSNSVGW 239
Score = 43.2 bits (88), Expect(2) = 1e-042
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = -1 / +3
Query: 370 KTSNGPSGVSVKPLPAVKEKVKRMMFYC* 284
++S V+V+PLPA+KE VKR+MFYC*
Sbjct: 537 RSSTRFGNVAVRPLPALKENVKRVMFYC* 623
Score = 27.6 bits (54), Expect(2) = 7e-024
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / -3
Query: 288 Q*NIILFTFSFTAGRGFTET 347
Q*N+ TFS AGRG T T
Sbjct: 619 Q*NMTRLTFSLRAGRGLTAT 560
>gb|BJ473415.1|BJ473415 BJ473415 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal12f16 3', mRNA sequence
Length = 588
Score = 149 bits (321), Expect(2) = 1e-042
Identities = 59/84 (70%), Positives = 62/84 (73%)
Frame = -1 / -3
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDEN FTADE+Q L NNLCYTYARCTRSV
Sbjct: 523 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIV 344
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDN 380
RFYMEP+ D+
Sbjct: 343 PPAYYAHLAAFRARFYMEPDTSDS 272
Score = 102 bits (217), Expect(2) = 7e-024
Identities = 53/83 (63%), Positives = 57/83 (68%)
Frame = +3 / +3
Query: 381 LSNISGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE 560
LS +SGSM + A*YAGGT TERVHRA V* RLF C SSAV LFSSQRT
Sbjct: 273 LSEVSGSM*NRARKAARWA*YAGGTIDTERVHRAYV*HRLFVRVCNSSAVNLFSSQRT** 452
Query: 561 *AGLLVPWIPA*LQR*KSNSVGW 629
AG LVP +PA L +*KSNSVGW
Sbjct: 453 WAGRLVP*MPAWLHK*KSNSVGW 521
Score = 43.2 bits (88), Expect(2) = 1e-042
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = -1 / -3
Query: 370 KTSNGPSGVSVKPLPAVKEKVKRMMFYC* 284
++S V+V+PLPA+KE VKR+MFYC*
Sbjct: 223 RSSTRFGNVAVRPLPALKENVKRVMFYC* 137
Score = 27.6 bits (54), Expect(2) = 7e-024
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / +3
Query: 288 Q*NIILFTFSFTAGRGFTET 347
Q*N+ TFS AGRG T T
Sbjct: 141 Q*NMTRLTFSLRAGRGLTAT 200
>gb|AV916245.1|AV916245 AV916245 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags12e13 5', mRNA sequence
Length = 481
Score = 152 bits (327), Expect = 1e-036
Identities = 60/81 (74%), Positives = 62/81 (76%)
Frame = -1 / +1
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDENNF+ADEMQ L NNLCYTYARCTRSV
Sbjct: 238 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFSADEMQTLTNNLCYTYARCTRSVSVV 417
Query: 451 XXXXXXXXXXXXXRFYMEPEM 389
RFYMEPE+
Sbjct: 418 PPAYYAHLAAFRARFYMEPEL 480
Score = 145 bits (312), Expect = 2e-034
Identities = 56/81 (69%), Positives = 63/81 (77%)
Frame = +1 / -1
Query: 388 TSLVPCRTEPAMLPSVRSMQEGQHKPSVCTGHKCSKGCLAMAAFRLL*NCSHPRGHGSEQ 567
T+ PCRT P M P R+MQ G+ K S CT KCSKGCL+ +AFRLL +CSHPRGHGSE
Sbjct: 481 TAPAPCRTAPGMQPDARNMQVGRRKQSACTVRKCSKGCLSTSAFRLLRSCSHPRGHGSEL 302
Query: 568 AYSFPGFQHDYRGRNQTPLGG 630
AY +PGFQ DYRGRNQTPLGG
Sbjct: 301 AYLYPGFQRDYRGRNQTPLGG 239
Score = 142 bits (304), Expect = 2e-033
Identities = 65/79 (82%), Positives = 67/79 (84%)
Frame = +3 / -3
Query: 393 SGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE*AGL 572
SGSM +CA*YAGGTT+TERVHRA*V*QRLF N CISSA KLFSSQRTW *AGL
Sbjct: 476 SGSM*NRARNAARCA*YAGGTTETERVHRA*V*QRLFVNVCISSAEKLFSSQRTW**AGL 297
Query: 573 LVPWIPA*LQR*KSNSVGW 629
LVPWIPA*LQR*KSNSVGW
Sbjct: 296 LVPWIPA*LQR*KSNSVGW 240
Score = 123 bits (264), Expect = 7e-028
Identities = 55/77 (71%), Positives = 59/77 (76%)
Frame = -3 / +3
Query: 629 PPNGV*FLPL*SCWNPGNE*ACSLPCPLG*EQFHSRRNAAIAKQPLLHLCPVHTLGLCCP 450
PPNGV*FLPL*S WNPG +*A SLPCPLG*EQ SRRNA + KQPLLHL VH L P
Sbjct: 240 PPNGV*FLPL*SRWNPGYK*ASSLPCPLG*EQLLSRRNADVDKQPLLHLRTVHALCFRRP 419
Query: 449 SCILRTLGSIAGSVLHG 399
+CILR G I G+VLHG
Sbjct: 420 TCILRASGCIPGAVLHG 470
Score = 122 bits (262), Expect = 1e-027
Identities = 56/81 (69%), Positives = 59/81 (72%)
Frame = -2 / +2
Query: 630 ATQRSLISTSVVMLESRERVGLLTPMSSGMRTISQQTKCSHCQTTFATLMPGAHARFVLS 451
ATQRSLISTSVV LESR +VG LT MSSGMRT SQQTKC QTTFATL GA A F S
Sbjct: 239 ATQRSLISTSVVTLESRVQVGQLTTMSSGMRTTSQQTKCRR*QTTFATLTHGARALFPSS 418
Query: 450 LLHTTHTWQHCGLGSTWNQRC 388
LH T W H G GSTW++ C
Sbjct: 419 HLHITRIWLHSGRGSTWSRSC 481
Score = 111 bits (238), Expect = 3e-024
Identities = 53/80 (66%), Positives = 58/80 (72%)
Frame = +2 / -2
Query: 392 LWFHVEPSPXXXXXXXXXRRDNTNRACAPGISVAKVVWQWLHFVCCEIVLIPEDMGVSRP 571
L HVEP P R D+ NRA AP +SVAKVV Q LHFVC E+VLIPEDM VS P
Sbjct: 477 LRLHVEPRPECSQMRVICRWDDGNRARAPCVSVAKVVCQRLHFVC*EVVLIPEDMVVSWP 298
Query: 572 TRSLDSSMTTEVEIKLRWVA 631
T +LDSS+TTEVEIKLRWVA
Sbjct: 297 TCTLDSSVTTEVEIKLRWVA 238
>gb|BJ451080.1|BJ451080 BJ451080 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak32j21 5', mRNA sequence
Length = 576
Score = 144 bits (309), Expect = 4e-034
Identities = 58/83 (69%), Positives = 60/83 (72%)
Frame = -1 / +1
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDFYLCSHAGIQGTSRPAH HVLWDENNFTAD +Q L NNLCYTYARCTRSV
Sbjct: 232 CHPTEFDFYLCSHAGIQGTSRPAHYHVLWDENNFTADGLQTLTNNLCYTYARCTRSVSIV 411
Query: 451 XXXXXXXXXXXXXRFYMEPEMLD 383
RFYME + D
Sbjct: 412 PPAYYAHLAAFRARFYMELDTSD 480
Score = 107 bits (229), Expect = 5e-023
Identities = 55/82 (67%), Positives = 57/82 (69%)
Frame = +3 / -2
Query: 384 SNISGSMXXXXXXXXKCA*YAGGTTQTERVHRA*V*QRLFGNGCISSAVKLFSSQRTWE* 563
S +S SM + A*YAGGT TERVH A*V* RLF C SAVKLFSSQRTW
Sbjct: 479 SEVSSSM*NRARKAAR*A*YAGGTIDTERVHLA*V*HRLFVRVCKPSAVKLFSSQRTW*C 300
Query: 564 AGLLVPWIPA*LQR*KSNSVGW 629
AG LVP IPA LQR*KSNSVGW
Sbjct: 299 AGRLVP*IPAWLQR*KSNSVGW 234
>gb|CB875233.1|CB875233 HX07K22w HX Hordeum vulgare subsp. vulgare cDNA clone HX07K22
3-PRIME, mRNA sequence
Length = 603
Score = 116 bits (249), Expect(2) = 2e-033
Identities = 48/73 (65%), Positives = 51/73 (69%)
Frame = -1 / -3
Query: 598 SHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXXXXXXXXXXXXX 419
SHAGIQGTSRPAH HVLWDEN FTADE+Q L NNLCYTYARCTRSV
Sbjct: 601 SHAGIQGTSRPAHYHVLWDENKFTADELQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAF 422
Query: 418 XXRFYMEPEMLDN 380
RFYMEP+ D+
Sbjct: 421 RARFYMEPDTSDS 383
Score = 45.5 bits (93), Expect(2) = 2e-033
Identities = 18/34 (52%), Positives = 24/34 (70%)
Frame = -1 / -3
Query: 379 QTSKTSNGPSGVSVKPLPAVKEKVKRMMFYC*QG 278
Q + + V+V+PLPA+KE VKR+MFYC* G
Sbjct: 349 QGGRNNRAFGNVAVRPLPALKENVKRVMFYC*GG 248
>gb|BU973725.1|BU973725 HB25M17r BC Hordeum vulgare subsp. vulgare cDNA clone HB25M17
5-PRIME, mRNA sequence
Length = 506
Score = 127 bits (272), Expect(2) = 4e-033
Identities = 54/90 (60%), Positives = 58/90 (64%)
Frame = -1 / +1
Query: 625 PTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXXXX 446
PTEFDFYLCSHAGIQGTSRP H HVL+DEN FTAD +Q L NNLCYTYARCTRSV
Sbjct: 13 PTEFDFYLCSHAGIQGTSRPTHYHVLFDENGFTADGLQQLTNNLCYTYARCTRSVSVVPP 192
Query: 445 XXXXXXXXXXXRFYMEPEMLDNQTSKTSNG 356
R+Y EP + S S G
Sbjct: 193 AYYAHLAAFRARYYDEPSEGSDSASIVSGG 282
Score = 33.6 bits (67), Expect(2) = 4e-033
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = -1 / +1
Query: 337 KPLPAVKEKVKRMMFYC*QG 278
+PLP +++ VK +MFYC* G
Sbjct: 337 RPLPRIRDNVKEVMFYC*IG 396
>gb|BM100617.2|BM100617 EBma01_SQ005_L23_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ005_L23 5', mRNA sequence
Length = 670
Score = 130 bits (278), Expect = 8e-030
Identities = 49/57 (85%), Positives = 51/57 (89%)
Frame = -1 / +3
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSV 461
CHPTEFDFYLCSHAGIQGTSRP H HVL+DEN FTAD +Q L NNLCYTYARCTRSV
Sbjct: 456 CHPTEFDFYLCSHAGIQGTSRPTHYHVLFDENGFTADGLQQLTNNLCYTYARCTRSV 626
>gb|BU996805.1|BU996805 HM14N04r HM Hordeum vulgare subsp. vulgare cDNA clone HM14N04
5-PRIME, mRNA sequence
Length = 612
Score = 126 bits (269), Expect = 1e-028
Identities = 49/77 (63%), Positives = 56/77 (72%)
Frame = -1 / +2
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHPTEFDF+LCSHAGI+GTSRPAH HVL D+NNF+AD++Q L NNLCYTYA CTRSV
Sbjct: 314 CHPTEFDFFLCSHAGIKGTSRPAHYHVLRDDNNFSADDLQSLTNNLCYTYASCTRSVSTA 493
Query: 451 XXXXXXXXXXXXXRFYM 401
RFY+
Sbjct: 494 PPAYYAHKLAFRARFYL 544
>gb|BU971142.1|BU971142 HB16L19r BC Hordeum vulgare subsp. vulgare cDNA clone HB16L19
5-PRIME, mRNA sequence
Length = 470
Score = 124 bits (265), Expect = 5e-028
Identities = 46/54 (85%), Positives = 48/54 (88%)
Frame = -1 / +1
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCT 470
CHPTEFDFYLCSHAGIQGTSRP H HVL+DEN FTAD +Q L NNLCYTYARCT
Sbjct: 307 CHPTEFDFYLCSHAGIQGTSRPTHYHVLFDENGFTADGLQQLTNNLCYTYARCT 468
>gb|BE215344.1|BE215344 HV_CEb0006I15f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0006I15f, mRNA sequence
Length = 679
Score = 120 bits (257), Expect = 6e-027
Identities = 45/57 (78%), Positives = 49/57 (85%)
Frame = -1 / +2
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSV 461
CHPTEFDFYLC+HAGIQGTSRPA+ HVLWDEN FTADE+Q L LCY YA+ TRSV
Sbjct: 413 CHPTEFDFYLCNHAGIQGTSRPANYHVLWDENKFTADELQTLKKKLCYNYAKGTRSV 583
>gb|BJ457634.1|BJ457634 BJ457634 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak28j10 3', mRNA sequence
Length = 463
Score = 89.5 bits (189), Expect(2) = 1e-022
Identities = 37/61 (60%), Positives = 39/61 (63%)
Frame = -1 / -2
Query: 565 AHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXXXXXXXXXXXXXXXRFYMEPEML 386
AH HVLWDENNFTAD +Q L NNLCYTYARCTRSV RFYMEP+
Sbjct: 462 AHYHVLWDENNFTADGLQTLTNNLCYTYARCTRSVSIVPPAYYAHLAAFRARFYMEPDTS 283
Query: 385 D 383
D
Sbjct: 282 D 280
Score = 36.3 bits (73), Expect(2) = 1e-022
Identities = 15/27 (55%), Positives = 18/27 (66%)
Frame = -1 / -2
Query: 364 SNGPSGVSVKPLPAVKEKVKRMMFYC* 284
S V+VKPLP + VKR+MFYC*
Sbjct: 219 SRAAGNVAVKPLPDLN*NVKRVMFYC* 139
>gb|BE195976.3|BE195976 HVSMEh0091A07f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0091A07f, mRNA sequence
Length = 1009
Score = 94.5 bits (200), Expect(2) = 6e-022
Identities = 38/102 (37%), Positives = 54/102 (52%)
Frame = -1 / +1
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHP FDFY+C+HAG+ GTSRP H HVL DE F+ADE+Q ++L Y Y R T ++
Sbjct: 208 CHPKNFDFYMCAHAGMIGTSRPTHYHVLHDEIGFSADELQEFVHSLSYVYQRSTTAISVA 387
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDNQTSKTSNGPSGVSVKPLP 326
+M+ E + + +S G + V +P
Sbjct: 388 APIAYAHLAAAQVGTFMKFEDMSDTSSSQGGGHTSVGSASVP 513
Score = 29.0 bits (57), Expect(2) = 6e-022
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = -1 / +1
Query: 343 SVKPLPAVKEKVKRMMFYC* 284
SV LP + EKVK MF+C*
Sbjct: 505 SVPELPRLHEKVKSSMFFC* 564
>gb|BU975642.1|BU975642 HB31N05r BC Hordeum vulgare subsp. vulgare cDNA clone HB31N05
5-PRIME, mRNA sequence
Length = 617
Score = 94.5 bits (200), Expect(2) = 1e-021
Identities = 38/102 (37%), Positives = 54/102 (52%)
Frame = -1 / +2
Query: 631 CHPTEFDFYLCSHAGIQGTSRPAHSHVLWDENNFTADEMQPLPNNLCYTYARCTRSVCXX 452
CHP FDFY+C+HAG+ GTSRP H HVL DE F+ADE+Q ++L Y Y R T ++
Sbjct: 152 CHPKNFDFYMCAHAGMIGTSRPTHYHVLHDEIGFSADELQEFVHSLSYVYQRSTTAISVA 331
Query: 451 XXXXXXXXXXXXXRFYMEPEMLDNQTSKTSNGPSGVSVKPLP 326
+M+ E + + +S G + V +P
Sbjct: 332 APIAYAHLAAAQVGTFMKFEDMSDTSSSQGGGHTSVGSASVP 457
Score = 27.6 bits (54), Expect(2) = 1e-021
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -1 / +2
Query: 343 SVKPLPAVKEKVKRMMFYC* 284
SV LP + EKV+ MF+C*
Sbjct: 449 SVPELPRLHEKVRSSMFFC* 508
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 266,044,545
Number of Sequences: 312970
Number of extensions: 3927724
Number of successful extensions: 43044
Number of sequences better than 1.0e-020: 24
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6701840022
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)