TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3191506.2.1
(1208 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU973760.1|BU973760 HB25O06r BC Hordeum vulgare subsp. v... 420 e-117
gb|AL506751.1|AL506751 AL506751 Hordeum vulgare Barke devel... 328 e-104
gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. v... 318 5e-086
gb|AV833832.1|AV833832 AV833832 K. Sato unpublished cDNA li... 300 9e-081
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 281 7e-075
gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgar... 145 2e-068
gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA li... 133 3e-063
gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA li... 133 9e-063
gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. v... 231 8e-060
gb|BI950903.1|BI950903 HVSMEl0023J16f Hordeum vulgare spike... 211 7e-054
gb|CK122439.1|CK122439 BES1824103h01 BES1824 Hordeum vulgar... 80 2e-048
gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. v... 122 7e-047
gb|BQ759105.1|BQ759105 EBma07_SQ003_E23_R maternal, 21 DPA,... 164 5e-044
gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-a... 149 1e-042
gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-a... 149 1e-042
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 170 2e-041
gb|BU995595.1|BU995595 HM10M02r HM Hordeum vulgare subsp. v... 133 3e-039
gb|BF266558.3|BF266558 HV_CEa0015H07f Hordeum vulgare seedl... 149 5e-038
gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, dro... 122 1e-037
gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA li... 151 8e-036
gb|AV836570.1|AV836570 AV836570 K. Sato unpublished cDNA li... 135 5e-031
gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day ger... 133 1e-030
gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day ger... 133 1e-030
gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day ger... 133 1e-030
gb|BU988833.1|BU988833 HF18N17r HF Hordeum vulgare subsp. v... 95 1e-028
gb|CA012566.1|CA012566 HT05M19r HT Hordeum vulgare subsp. v... 100 1e-028
gb|AL450785.1|AL450785 AL450785 Hordeum vulgare Barke etiol... 90 4e-024
gb|BE455944.3|BE455944 HVSMEg0017N04f Hordeum vulgare pre-a... 109 4e-023
gb|AV836656.1|AV836656 AV836656 K. Sato unpublished cDNA li... 105 5e-022
>gb|BU973760.1|BU973760 HB25O06r BC Hordeum vulgare subsp. vulgare cDNA clone HB25O06
5-PRIME, mRNA sequence
Length = 563
Score = 420 bits (912), Expect = e-117
Identities = 170/184 (92%), Positives = 177/184 (96%)
Frame = +3 / +1
Query: 570 DYYLLSPEKRKFPNSENLENPDLYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTD 749
DYYLL EKRKFP SENLENP+LYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTD
Sbjct: 10 DYYLLPLEKRKFPRSENLENPELYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTD 189
Query: 750 KLNFGAMNMWFLLNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRPKT 929
KLNFGAMNMWFLLNPPG ATIHVENVD+FKWLNSSYCPVL+QLES AMKEYYFKADRP T
Sbjct: 190 KLNFGAMNMWFLLNPPGKATIHVENVDEFKWLNSSYCPVLRQLESVAMKEYYFKADRPAT 369
Query: 930 LSAGSSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGN 1109
LSAGSSNLKYRNPKYLSMLNHLRFYLPQ+YPKL+KILFLDDDIVVQ+DLTGLW+VDLNG
Sbjct: 370 LSAGSSNLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGM 549
Query: 1110 VNGA 1121
VNGA
Sbjct: 550 VNGA 561
Score = 315 bits (682), Expect = 3e-085
Identities = 141/185 (76%), Positives = 153/185 (82%)
Frame = -2 / -1
Query: 1123 TAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMDKYFGFRYFRLE 944
TAP T P RSTS +PV+S TT+SSSRK IL + G* CGR*NL+ SM++Y G RYFR E
Sbjct: 563 TAPLTIPFRSTSHNPVKSFCTTMSSSRKSILSNFG*ICGR*NLKWFSMERYLGLRYFRFE 384
Query: 943 EPAESVFGRSALK*YSFMAADSSCFRTGQ*EEFNHLKSSTFSTWIVASPGGFSKNHMFMA 764
EPAE V GRSALK*YSFMA DSS +TGQ +EFNHL SSTFSTWIVA PGGFSKNHMFMA
Sbjct: 383 EPAERVAGRSALK*YSFMATDSS*RKTGQYDEFNHLNSSTFSTWIVALPGGFSKNHMFMA 204
Query: 763 PKFSLSVTR*NTCFSGSLAFMMVELTTTDAAKTLSEKRA*W*RSGFSRFSLFGNFLFSGE 584
PKF+LSVTR TCFSGSLAFMMVE TTT+AAKTLSEK A*W* SGFS+FSL GNF FS
Sbjct: 203 PKFNLSVTRWKTCFSGSLAFMMVEFTTTEAAKTLSEKSA*W*SSGFSKFSLLGNFRFSRG 24
Query: 583 RR**S 569
RR**S
Sbjct: 23 RR**S 9
Score = 211 bits (455), Expect = 7e-054
Identities = 96/179 (53%), Positives = 131/179 (73%)
Frame = -1 / -3
Query: 1106 SIKINLPESS*VPLDNYIIIQEKNFIQLGIDLWEVKS*MIEHGQIFWVSILQIRRTSREC 927
SI+IN+P+S + L NY+II+EK IQL IDLWE+KS +++HG++ V++ + RT +
Sbjct: 546 SIQINIPQSCQIFLHNYVIIEEKYLIQLWIDLWEIKSQVVQHGEVLGVAVF*V*RTC*KS 367
Query: 926 FRTISLEIIFFHGCRLKLLQNRAVRRV*PFEVINIFHMDCCIPRWIQQKPHVHGPKVQFV 747
T+SLEII FHG RL+L NR V V P ++I+I +MD + RW+QQKPHVHG KVQ +
Sbjct: 366 GGTVSLEIILFHGNRLQLT*NRTV**VQPLKLIHILNMDSGLARWVQQKPHVHGSKVQLI 187
Query: 746 SNKMKYMFFRLLSIHDG*VDHN*CCQNIVREKSIMVKIRIFQVLTIWELSFLWREKIII 570
+KM+ MF RLLSIHDG*V +N Q+IV EK I+V++ IFQV T EL FL R++I+I
Sbjct: 186 GHKMENMFLRLLSIHDG*VYNNRGSQDIV*EKCIVVELWIFQVFTPRELPFLQRKEIVI 10
Score = 186 bits (400), Expect = 3e-046
Identities = 80/161 (49%), Positives = 110/161 (68%)
Frame = +2 / +3
Query: 635 SLPLCSFLGQCFGSISCGQLNHHEC*GA*KTCISSCY*QTELWGHEHVVFAESTWGCNNP 814
+LPLC+FL QC G + C +LNHHEC*GA*+TC SC ELW HEHVVFAE TW + P
Sbjct: 75 ALPLCTFLRQCLGCLCCCKLNHHEC*GA*ETCFPSCDR*VELWSHEHVVFAEPTWQGHYP 254
Query: 815 CGKC**LQMVKLFLLPCSEAA*VCSHERILFQG*SSENTLCWFF*SEVSKPKIFVHAQSS 994
C +C *+ +V+L +L C + VC HER+LFQG* S ++ FF ++ +P++ +HA+
Sbjct: 255 C*ECG*V*VVELIILSCFTSTGVCCHERVLFQG*PSRHSFSRFFKPKIPQPQVPLHAEPL 434
Query: 995 KILPPTSLSQAE*NSFPG**YSCPEGPNWTLGG*S*WKCKW 1117
+IL PT LS+ *++FP **+SC E + +G *S W +W
Sbjct: 435 EILSPTDLSKVG*DTFPR**HSCAERFDRIVGC*SEWNGQW 557
Score = 178 bits (383), Expect = 6e-044
Identities = 90/182 (49%), Positives = 100/182 (54%)
Frame = -3 / -2
Query: 1116 HLHFH*DQPPRVQLGPSGQLYHHPGKEFYSAWDRLVGGKILDD*AWTNILGFDTSD*KNQ 937
H FH DQ P + S QL HH GK Y DR VG KI AW G KN
Sbjct: 556 H*PFHSDQHPTILSNLSAQLCHHRGKVSYPTLDRSVGDKISSGSAWRGTWGCGILGLKNL 377
Query: 936 QRVFSDDQP*NNILSWLQTQAASEQGSKKSLTI*SHQHFPHGLLHPQVDSAKTTCSWPQS 757
+ + D QP*NN LSW QT +Q S S T *+H H HG QV SAKTTCSW QS
Sbjct: 376 LKEWRDGQP*NNTLSWQQTPVDVKQDSMMSSTT*THPHSQHG*WPCQVGSAKTTCSWLQS 197
Query: 756 SVCQ*QDEIHVFQAP*HS*WLS*PQLMLPKHCPRKEHNGKDQDFPGSHYLGTFFSLERED 577
S + QD HV QAP*HS*WLS Q P+HC RK H+G+ DFP H GT S E D
Sbjct: 196 STYRSQDGKHVSQAP*HS*WLSLQQQRQPRHCLRKVHSGRALDFPSFHSSGTSVSPEEGD 17
Query: 576 NN 571
+N
Sbjct: 16 SN 11
Score = 88.6 bits (187), Expect(2) = 6e-030
Identities = 47/91 (51%), Positives = 55/91 (60%)
Frame = +1 / +2
Query: 571 IIIFSLQRKESSQIVRTWKILIFTIMLFSRTMFWQHQLWSTQPS*MLRSLKNMYFIXXXX 750
I I L R SS+ V+TWKI T M FS+TM W L TQPS*MLRSL+NM+ I
Sbjct: 11 ITISFLWRNGSSRGVKTWKIQSSTTMHFSQTMSWLPLLL*TQPS*MLRSLRNMFSIL*PI 190
Query: 751 XXXXGP*TCGFC*IHLGMQQSMWKMLMTSNG 843
P*TCGFC* HL SM +M M+ +G
Sbjct: 191 S*TLEP*TCGFC*THLARPLSMLRMWMSLSG 283
Score = 62.9 bits (131), Expect(2) = 6e-030
Identities = 38/73 (52%), Positives = 44/73 (60%)
Frame = +1 / +2
Query: 889 P*KNIISRLIVRKHSLLVLLI*SIETQNICPCSII*DFTSHKSIPS*IKFFSWMMI*LSR 1068
P*K+IISRL V VL *+ T + PC *DF SH+SI S I++FS MM *L R
Sbjct: 329 P*KSIISRLTVPPLFQQVLQT*NTATPSTSPC*TT*DFISHRSIQSWIRYFSSMMT*LCR 508
Query: 1069 GT*LDSGRLILME 1107
* D G LI ME
Sbjct: 509 KI*QDCGMLI*ME 547
>gb|AL506751.1|AL506751 AL506751 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04C08T 5',
mRNA sequence
Length = 691
Score = 328 bits (710), Expect(2) = e-104
Identities = 143/175 (81%), Positives = 159/175 (90%)
Frame = +3 / +2
Query: 84 LVKLENAGIERSKAVDSAVLGKYSIWRRENENEKADSRVRLMRDQMIMARIYSVLAKSRD 263
LVKLENA IERSKAVDSAVLGKYSIWR+ENENE +DS VRLMRDQ+IMAR+YSVLAKSR+
Sbjct: 2 LVKLENAAIERSKAVDSAVLGKYSIWRKENENENSDSTVRLMRDQIIMARVYSVLAKSRN 181
Query: 264 KLDLYQELLARLKESQRSLGEATADAELPKSASDRTKAMGQVLSKARDLLYDCKEITQRL 443
K +YQEL +R+KESQR++GEATADA+L SA D+ + MGQVL+KAR+ LYDCK I+QRL
Sbjct: 182 KHGIYQELQSRIKESQRAVGEATADADLHHSAPDKIRVMGQVLTKAREELYDCKVISQRL 361
Query: 444 RAMLQSADEQVRSLKKQSTFLSQLAAKTIPNGIHCLSMRLTIDYYLLSPEKRKFP 608
RAMLQSADEQVRSLKKQSTFLSQLAAKTIPN IHCLSMRLTIDYYLL EKRKFP
Sbjct: 362 RAMLQSADEQVRSLKKQSTFLSQLAAKTIPNSIHCLSMRLTIDYYLLPLEKRKFP 526
Score = 166 bits (358), Expect(2) = 9e-049
Identities = 85/173 (49%), Positives = 111/173 (64%)
Frame = -1 / -1
Query: 605 ELSFLWREKIIINR*AHGKTMDAIWDCLSC*LAKEGALLLQAPDLLIC*LKHCSQTLGNF 426
EL FL R+ I+I *AH + +DA+W+ L LAK+ LLLQAPDL IC*LKHCS +L
Sbjct: 523 ELPFLQRKXIVIYC*AHRQAVDAVWNRLCSQLAKKCTLLLQAPDLFIC*LKHCS*SLTYH 344
Query: 425 LAIVQ*IPCF**NLAHCFGSIRSTLGKFSISSSFPKGSLAFLEPCKQLLIEIKLVPRFGK 246
LAI+Q *NL H +R T+ K SI SS LAFL+ Q LI+ LVP F K
Sbjct: 343 LAIIQFFSGLR*NLTHHSDFVRCTVMKVSICSSLSYSPLAFLDSALQFLIDTMLVP*FCK 164
Query: 245 NRIYSGHDHLISHQTDP*ICLFIFIFTSPDAVFSQHSRVNSF*TFDTCILKFN 87
+R+ +GH++L+ HQ D *I + I I SP AVF + SR+ S *+ ++CIL+ N
Sbjct: 163 HRVNTGHNNLVPHQPDC*I*VLILILFSPYAVFPKDSRIYSL*SLNSCILQLN 5
Score = 129 bits (276), Expect(3) = 1e-058
Identities = 63/107 (58%), Positives = 69/107 (64%)
Frame = -2 / -2
Query: 628 FSRFSLFGNFLFSGERR**SIVKRMERQWMPFGIVLAASWLRKVLCFFKLRTCSSAD*SI 449
F F GNF FS R**SIVKR+++QWM FGIV AA+WLR VLCFFKL TCSSAD*SI
Sbjct: 546 FPSFHSLGNFRFSRGXR**SIVKRIDKQWMLFGIVFAANWLRNVLCFFKLLTCSSAD*SI 367
Query: 448 ALKRWVISLQSYNRSLAFDKTWPIXXXXXXXXXXXXXXXXXSPRDRW 308
AL +I+LQSYN SLAF KT PI SP RW
Sbjct: 366 ALSL*LITLQSYNSSLAFVKT*PITLILSGAL**RSASAVASPTARW 226
Score = 95.0 bits (201), Expect(3) = 3e-028
Identities = 54/123 (43%), Positives = 73/123 (59%)
Frame = +1 / +3
Query: 88 LNLRMQVSNVQKLLTLLCWENTASGDVKMKMKRQIQGSV*CEIK*SWPEYILFLPNRGTS 267
L+ RMQ+ + Q+L LL NTA G+ +M+M+ QIQ S * K WP + L L N+GTS
Sbjct: 6 LSWRMQLLSDQRL*ILLSLGNTAYGEKRMRMRTQIQQSG**GTKLLWPVFTLCLQNQGTS 185
Query: 268 LISIRSCLQGSRKASDPLGKLLLMLNFPRVLRIEPKQWAKFYQKQGIYCTIARKLPSV*E 447
++SI++C SRKAS +LL ML F V + + W KF ++ C IAR *E
Sbjct: 186 MVSIKNCRAESRKASGL*ERLLQMLTFITVHLTKSE*WVKF*RRPEKNCMIAR**VRD*E 365
Query: 448 QCF 456
QCF
Sbjct: 366 QCF 374
Score = 80.8 bits (170), Expect(3) = 1e-023
Identities = 44/96 (45%), Positives = 55/96 (57%)
Frame = +2 / +1
Query: 188 RFKGPFDARSNDHGQNIFCSCQIEGQA*SLSGAACKAQGKPAIPWGSYC*C*TSQECFG* 367
RF D N +G + C+C+I+ QA LS A QGKPA YC C* S +C
Sbjct: 106 RFNSQVDEGPNYYGPCLLCACKIKEQAWYLSRTAEPNQGKPAGCRRGYCRC*PSSQCT*Q 285
Query: 368 NQSNGPSFIKSKGSIVRLQGNYPAFESNASVSR*AG 475
NQS+G SF + + IV LQG+ +SNASVSR* G
Sbjct: 286 NQSDGSSFNEGQRRIV*LQGDKSETKSNASVSR*TG 393
Score = 78.0 bits (164), Expect(3) = 1e-058
Identities = 38/54 (70%), Positives = 42/54 (77%)
Frame = -2 / -2
Query: 244 TEYILAMII*SRIKRTLESAFSFSFSRLQMLYFPSTAESTAFERSIPAFSSLTN 83
TE A+II S I T+ES FSFSFS L MLYFP TAESTAF+RSI AFSSLT+
Sbjct: 162 TE*TRAIIIWSLINLTVESEFSFSFSFLHMLYFPRTAESTAFDRSIAAFSSLTS 1
Score = 72.1 bits (151), Expect(2) = e-104
Identities = 30/31 (96%), Positives = 31/31 (100%)
Frame = +3 / +3
Query: 612 SENLENPDLYHYALFSDNVLAASVVVNSTIM 704
SENLENP+LYHYALFSDNVLAASVVVNSTIM
Sbjct: 531 SENLENPELYHYALFSDNVLAASVVVNSTIM 623
Score = 58.8 bits (122), Expect(3) = 1e-058
Identities = 26/31 (83%), Positives = 28/31 (90%)
Frame = -2 / -1
Query: 703 MMVELTTTDAAKTLSEKRA*W*RSGFSRFSL 611
MMVE TTT+AAKTLSEK A*W* SGFS+FSL
Sbjct: 622 MMVEXTTTEAAKTLSEKSA*W*SSGFSKFSL 530
Score = 47.8 bits (98), Expect(2) = 9e-049
Identities = 19/35 (54%), Positives = 22/35 (62%)
Frame = -3 / -2
Query: 702 *WLS*PQLMLPKHCPRKEHNGKDQDFPGSHYLGTF 598
*WLS Q P+HC RK H+G+ DFP H LG F
Sbjct: 621 *WLSXQQQRQPRHCLRKVHSGRALDFPSFHSLGNF 517
Score = 41.8 bits (85), Expect(3) = 3e-028
Identities = 14/23 (60%), Positives = 17/23 (73%)
Frame = +2 / +2
Query: 635 SLPLCSFLGQCFGSISCGQLNHH 703
+LPLC+FL QC G + C LNHH
Sbjct: 554 ALPLCTFLRQCLGCLCCCXLNHH 622
Score = 38.6 bits (78), Expect(3) = 1e-023
Identities = 17/30 (56%), Positives = 19/30 (63%)
Frame = +1 / +1
Query: 613 VRTWKILIFTIMLFSRTMFWQHQLWSTQPS 702
V+TWKI T M FS+TM W L TQPS
Sbjct: 532 VKTWKIQSSTTMHFSQTMSWLPLLLXTQPS 621
Score = 33.1 bits (66), Expect(3) = 1e-023
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +2 / +1
Query: 98 ECRYRTFKSC*LCCAGKIQHLE 163
EC Y K C CC +IQH+E
Sbjct: 16 ECSY*AIKGCRFCCPWEIQHME 81
Score = 26.7 bits (52), Expect(3) = 3e-028
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / +1
Query: 524 DNPKWHPLSFHALND*LLSSLS 589
D+ K HPL +ALN+ LLS S
Sbjct: 442 DDSKQHPLLVYALNNRLLSXSS 507
>gb|BU979822.1|BU979822 HA17H16r HA Hordeum vulgare subsp. vulgare cDNA clone HA17H16
5-PRIME, mRNA sequence
Length = 554
Score = 318 bits (688), Expect = 5e-086
Identities = 127/129 (98%), Positives = 128/129 (99%)
Frame = +3 / +3
Query: 771 NMWFLLNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRPKTLSAGSSN 950
NMWFLLNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADR KTLSAGSSN
Sbjct: 12 NMWFLLNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRQKTLSAGSSN 191
Query: 951 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVET 1130
LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQ+DLTGLWEVDLNGNVNGAVET
Sbjct: 192 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQKDLTGLWEVDLNGNVNGAVET 371
Query: 1131 CGESFHRFD 1157
CGESFHRFD
Sbjct: 372 CGESFHRFD 398
Score = 275 bits (594), Expect = 5e-073
Identities = 116/129 (89%), Positives = 123/129 (95%)
Frame = -2 / -2
Query: 1156 SNR*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMD 977
SNR KLSPHVSTAPFTFPLRSTS SPV+S WTT+SSSRKRILF+LG*TCGR*NL+*LSMD
Sbjct: 397 SNRWKLSPHVSTAPFTFPLRSTSHSPVKSFWTTMSSSRKRILFNLG*TCGR*NLK*LSMD 218
Query: 976 KYFGFRYFRLEEPAESVFGRSALK*YSFMAADSSCFRTGQ*EEFNHLKSSTFSTWIVASP 797
+YFGFRYFRLEEPAE VF RSALK*YSFMAADSSCF+TGQ EEFNHLKSSTFSTWIVASP
Sbjct: 217 RYFGFRYFRLEEPAERVF*RSALK*YSFMAADSSCFKTGQYEEFNHLKSSTFSTWIVASP 38
Query: 796 GGFSKNHMF 770
GGF+KNHMF
Sbjct: 37 GGFNKNHMF 11
Score = 215 bits (464), Expect = 4e-055
Identities = 91/129 (70%), Positives = 110/129 (85%)
Frame = -1 / -1
Query: 1157 IKSVKTLSTCFHGSIYISIKINLPESS*VPLDNYIIIQEKNFIQLGIDLWEVKS*MIEHG 978
+KSV+TL TCF+ ++YI IKINLP SS + LD+ +II+EK+FIQL IDLWEVKS*+IEHG
Sbjct: 398 VKSVETLPTCFYSTVYIPIKINLP*SSQILLDHNVIIEEKDFIQLRIDLWEVKS*VIEHG 219
Query: 977 QIFWVSILQIRRTSRECFRTISLEIIFFHGCRLKLLQNRAVRRV*PFEVINIFHMDCCIP 798
QIFWVS+ QIR T E F TI L+++ FHG RL+LLQNRAVRRV*PFEVI+IF+MDC IP
Sbjct: 218 QIFWVSVFQIRGTGGESFLTIRLKVVLFHGRRLELLQNRAVRRV*PFEVIHIFNMDCGIP 39
Query: 797 RWIQQKPHV 771
R IQQ+PHV
Sbjct: 38 RRIQQEPHV 12
Score = 200 bits (432), Expect = 1e-050
Identities = 88/129 (68%), Positives = 98/129 (75%)
Frame = -3 / -3
Query: 1155 QIGENSLHMFPRLHLHFH*DQPPRVQLGPSGQLYHHPGKEFYSAWDRLVGGKILDD*AWT 976
QIG NS HMF + LH H*DQPP VQ P G HH GK FYS DRLVGGKIL D*AWT
Sbjct: 396 QIGGNSPHMFLQHRLHSH*DQPPIVQSNPFGPQCHHRGKGFYST*DRLVGGKILSD*AWT 217
Query: 975 NILGFDTSD*KNQQRVFSDDQP*NNILSWLQTQAASEQGSKKSLTI*SHQHFPHGLLHPQ 796
+ILGF SD*+N++R F DD P*++ LSW QT+AAS+QGS KSLTI*SH HF HGL HPQ
Sbjct: 216 DILGFGISD*RNRRREFFDDPP*SSTLSWPQTRAASKQGSTKSLTI*SHPHFQHGLWHPQ 37
Query: 795 VDSAKTTCS 769
DS +TTCS
Sbjct: 36 EDSTRTTCS 10
Score = 198 bits (426), Expect = 7e-050
Identities = 88/129 (68%), Positives = 100/129 (77%)
Frame = +2 / +2
Query: 770 EHVVFAESTWGCNNPCGKC**LQMVKLFLLPCSEAA*VCSHERILFQG*SSENTLCWFF* 949
EHVV ES+WGC+NPC KC *LQMVKLF+LPC EAA VC HER+L G SS+N+L F *
Sbjct: 11 EHVVLVESSWGCHNPC*KCG*LQMVKLFVLPCFEAARVCGHERVLL*GGSSKNSLRRFL* 190
Query: 950 SEVSKPKIFVHAQSSKILPPTSLSQAE*NSFPG**YSCPEGPNWTLGG*S*WKCKWSRGN 1129
SE+ KPKI VHAQS KILPPTSLS E*N FP **+ P+G +WT+GG*S*W+CK N
Sbjct: 191 SEIPKPKISVHAQSLKILPPTSLS*VE*NPFPR**HCGPKGFDWTMGG*S*WECKRCCRN 370
Query: 1130 MWREFSPI* 1156
MW EF PI*
Sbjct: 371 MWGEFPPI* 397
Score = 140 bits (300), Expect = 2e-032
Identities = 78/128 (60%), Positives = 83/128 (64%)
Frame = +1 / +1
Query: 772 TCGFC*IHLGMQQSMWKMLMTSNGXXXXXXXXXXXXXXQP*KNIISRLIVRKHSLLVLLI 951
TCG C*I LGM QSM KM MTSNG +P*K+ R IV+K S V LI
Sbjct: 13 TCGSC*ILLGMPQSMLKMWMTSNG*TLRTALF*SSSSLRP*KSTTLRRIVKKLSPPVPLI 192
Query: 952 *SIETQNICPCSII*DFTSHKSIPS*IKFFSWMMI*LSRGT*LDSGRLILMEM*MEPWKH 1131
*+ ETQNICPCSI *DFTSHKSI S*IK FS MM S+ *LD GRLILM M* KH
Sbjct: 193 *NTETQNICPCSIT*DFTSHKSILS*IKSFSSMMTLWSKRI*LDYGRLILMGM*TVL*KH 372
Query: 1132 VERVFTDL 1155
V RV TDL
Sbjct: 373 VGRVSTDL 396
>gb|AV833832.1|AV833832 AV833832 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp. vulgare
cDNA clone bags20k03, mRNA sequence
Length = 526
Score = 300 bits (650), Expect = 9e-081
Identities = 118/131 (90%), Positives = 126/131 (96%)
Frame = +3 / +2
Query: 765 AMNMWFLLNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRPKTLSAGS 944
++NMWFLLNPPG ATIHVENVD+FKWLNSSYCPVL+QLES AMKEYYFKADRP TLSAGS
Sbjct: 29 SLNMWFLLNPPGKATIHVENVDEFKWLNSSYCPVLRQLESVAMKEYYFKADRPATLSAGS 208
Query: 945 SNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAV 1124
SNLKYRNPKYLSMLNHLRFYLPQ+YPKL+KILFLDDDIVVQ+DLTGLW+VDLNG VNGAV
Sbjct: 209 SNLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNGAV 388
Query: 1125 ETCGESFHRFD 1157
TCGESFHRFD
Sbjct: 389 FTCGESFHRFD 421
Score = 212 bits (457), Expect = 4e-054
Identities = 93/129 (72%), Positives = 103/129 (79%)
Frame = -2 / -2
Query: 1156 SNR*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMD 977
S R KLSP V+TAP T P RSTS +PV+S TT+SSSRK IL + G* CGR*NL+ SM+
Sbjct: 420 SKRWKLSPQVNTAPLTIPFRSTSHNPVKSFCTTMSSSRKSILSNFG*ICGR*NLKWFSME 241
Query: 976 KYFGFRYFRLEEPAESVFGRSALK*YSFMAADSSCFRTGQ*EEFNHLKSSTFSTWIVASP 797
+Y G RYFR EEPAE V GRSALK*YSFMA DSS +TGQ +EFNHL SSTFSTWIVA P
Sbjct: 240 RYLGLRYFRFEEPAERVAGRSALK*YSFMATDSS*RKTGQYDEFNHLNSSTFSTWIVALP 61
Query: 796 GGFSKNHMF 770
GGFSKNHMF
Sbjct: 60 GGFSKNHMF 34
Score = 149 bits (320), Expect = 3e-035
Identities = 66/133 (49%), Positives = 95/133 (71%)
Frame = -1 / -1
Query: 1157 IKSVKTLSTCFHGSIYISIKINLPESS*VPLDNYIIIQEKNFIQLGIDLWEVKS*MIEHG 978
+++V+TLST H +I SI+IN+P+S + L NY+II+EK IQL IDLWE+KS +++HG
Sbjct: 421 VETVETLSTGKHCTIDHSIQINIPQSCQIFLHNYVIIEEKYLIQLWIDLWEIKSQVVQHG 242
Query: 977 QIFWVSILQIRRTSRECFRTISLEIIFFHGCRLKLLQNRAVRRV*PFEVINIFHMDCCIP 798
++ V++ + RT + T+SLEII FHG RL+L NR V V P ++I+I +MD +
Sbjct: 241 EVLGVAVF*V*RTC*KSGGTVSLEIILFHGNRLQLT*NRTV**VQPLKLIHILNMDSGLA 62
Query: 797 RWIQQKPHVHGPK 759
RW+QQKPHV PK
Sbjct: 61 RWVQQKPHVQAPK 23
Score = 127 bits (271), Expect = 2e-028
Identities = 57/127 (44%), Positives = 83/127 (65%)
Frame = +2 / +1
Query: 770 EHVVFAESTWGCNNPCGKC**LQMVKLFLLPCSEAA*VCSHERILFQG*SSENTLCWFF* 949
EHVVFAE TW + PC +C *+ +V+L +L C + VC HER+LFQG* S ++ FF
Sbjct: 34 EHVVFAEPTWQGHYPC*ECG*V*VVELIILSCFTSTGVCCHERVLFQG*PSRHSFSRFFK 213
Query: 950 SEVSKPKIFVHAQSSKILPPTSLSQAE*NSFPG**YSCPEGPNWTLGG*S*WKCKWSRGN 1129
++ +P++ +HA+ +IL PT LS+ *++FP **+SC E + +G *S W +W
Sbjct: 214 PKIPQPQVPLHAEPLEILSPTDLSKVG*DTFPR**HSCAERFDRIVGC*SEWNGQWCSVY 393
Query: 1130 MWREFSP 1150
+WREF P
Sbjct: 394 LWREFPP 414
Score = 109 bits (232), Expect = 4e-023
Identities = 59/127 (46%), Positives = 64/127 (50%)
Frame = -3 / -3
Query: 1149 GENSLHMFPRLHLHFH*DQPPRVQLGPSGQLYHHPGKEFYSAWDRLVGGKILDD*AWTNI 970
G NSLH H FH DQ P + S QL HH GK Y DR VG KI AW
Sbjct: 413 GGNSLHR*TLHH*PFHSDQHPTILSNLSAQLCHHRGKVSYPTLDRSVGDKISSGSAWRGT 234
Query: 969 LGFDTSD*KNQQRVFSDDQP*NNILSWLQTQAASEQGSKKSLTI*SHQHFPHGLLHPQVD 790
G KN + + D QP*NN LSW QT +Q S S T *+H H HG QV
Sbjct: 233 WGCGILGLKNLLKEWRDGQP*NNTLSWQQTPVDVKQDSMMSSTT*THPHSQHG*WPCQVG 54
Query: 789 SAKTTCS 769
SAKTTCS
Sbjct: 53 SAKTTCS 33
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 281 bits (607), Expect = 7e-075
Identities = 113/115 (98%), Positives = 114/115 (99%)
Frame = +3 / +3
Query: 813 HVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRPKTLSAGSSNLKYRNPKYLSMLNH 992
HVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADR KTLSAGSSNLKYRNPKYLSMLNH
Sbjct: 12 HVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRQKTLSAGSSNLKYRNPKYLSMLNH 191
Query: 993 LRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVETCGESFHRFD 1157
LRFYLPQVYPKLNKILFLDDDIVVQ+DLTGLWEVDLNGNVNGAVETCGESFHRFD
Sbjct: 192 LRFYLPQVYPKLNKILFLDDDIVVQKDLTGLWEVDLNGNVNGAVETCGESFHRFD 356
Score = 242 bits (522), Expect = 4e-063
Identities = 103/115 (89%), Positives = 109/115 (94%)
Frame = -2 / -3
Query: 1156 SNR*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMD 977
SNR KLSPHVSTAPFTFPLRSTS SPV+S WTT+SSSRKRILF+LG*TCGR*NL+*LSMD
Sbjct: 355 SNRWKLSPHVSTAPFTFPLRSTSHSPVKSFWTTMSSSRKRILFNLG*TCGR*NLK*LSMD 176
Query: 976 KYFGFRYFRLEEPAESVFGRSALK*YSFMAADSSCFRTGQ*EEFNHLKSSTFSTW 812
+YFGFRYFRLEEPAE VF RSALK*YSFMAADSSCF+TGQ EEFNHLKSSTFSTW
Sbjct: 175 RYFGFRYFRLEEPAERVF*RSALK*YSFMAADSSCFKTGQYEEFNHLKSSTFSTW 11
Score = 191 bits (411), Expect = 8e-048
Identities = 81/118 (68%), Positives = 99/118 (83%)
Frame = -1 / -2
Query: 1157 IKSVKTLSTCFHGSIYISIKINLPESS*VPLDNYIIIQEKNFIQLGIDLWEVKS*MIEHG 978
+KSV+TL TCF+ ++YI IKINLP SS + LD+ +II+EK+FIQL IDLWEVKS*+IEHG
Sbjct: 356 VKSVETLPTCFYSTVYIPIKINLP*SSQILLDHNVIIEEKDFIQLRIDLWEVKS*VIEHG 177
Query: 977 QIFWVSILQIRRTSRECFRTISLEIIFFHGCRLKLLQNRAVRRV*PFEVINIFHMDCC 804
QIFWVS+ QIR T E F TI L+++ FHG RL+LLQNRAVRRV*PFEVI+IF+M C
Sbjct: 176 QIFWVSVFQIRGTGGESFLTIRLKVVLFHGRRLELLQNRAVRRV*PFEVIHIFNMASC 3
Score = 177 bits (382), Expect = 8e-044
Identities = 79/117 (67%), Positives = 89/117 (76%)
Frame = -3 / -1
Query: 1155 QIGENSLHMFPRLHLHFH*DQPPRVQLGPSGQLYHHPGKEFYSAWDRLVGGKILDD*AWT 976
QIG NS HMF + LH H*DQPP VQ P G HH GK FYS DRLVGGKIL D*AWT
Sbjct: 354 QIGGNSPHMFLQHRLHSH*DQPPIVQSNPFGPQCHHRGKGFYST*DRLVGGKILSD*AWT 175
Query: 975 NILGFDTSD*KNQQRVFSDDQP*NNILSWLQTQAASEQGSKKSLTI*SHQHFPHGLL 805
+ILGF SD*+N++R F DD P*++ LSW QT+AAS+QGS KSLTI*SH HF HGL+
Sbjct: 174 DILGFGISD*RNRRREFFDDPP*SSTLSWPQTRAASKQGSTKSLTI*SHPHFQHGLV 4
Score = 168 bits (362), Expect = 5e-041
Identities = 78/115 (67%), Positives = 88/115 (76%)
Frame = +2 / +2
Query: 812 PCGKC**LQMVKLFLLPCSEAA*VCSHERILFQG*SSENTLCWFF*SEVSKPKIFVHAQS 991
PC KC *LQMVKLF+LPC EAA VC HER+L G SS+N+L F *SE+ KPKI VHAQS
Sbjct: 11 PC*KCG*LQMVKLFVLPCFEAARVCGHERVLL*GGSSKNSLRRFL*SEIPKPKISVHAQS 190
Query: 992 SKILPPTSLSQAE*NSFPG**YSCPEGPNWTLGG*S*WKCKWSRGNMWREFSPI* 1156
KILPPTSLS E*N FP **+ P+G +WT+GG*S*W+CK NMW EF PI*
Sbjct: 191 LKILPPTSLS*VE*NPFPR**HCGPKGFDWTMGG*S*WECKRCCRNMWGEFPPI* 355
Score = 117 bits (251), Expect = 9e-026
Identities = 67/116 (57%), Positives = 74/116 (63%)
Frame = +1 / +1
Query: 808 QSMWKMLMTSNGXXXXXXXXXXXXXXQP*KNIISRLIVRKHSLLVLLI*SIETQNICPCS 987
++M KM MTSNG +P*K+ R IV+K S V LI*+ ETQNICPCS
Sbjct: 7 EAMLKMWMTSNG*TLRTALF*SSSSLRP*KSTTLRRIVKKLSPPVPLI*NTETQNICPCS 186
Query: 988 II*DFTSHKSIPS*IKFFSWMMI*LSRGT*LDSGRLILMEM*MEPWKHVERVFTDL 1155
I *DFTSHKSI S*IK FS MM S+ *LD GRLILM M* KHV RV TDL
Sbjct: 187 IT*DFTSHKSILS*IKSFSSMMTLWSKRI*LDYGRLILMGM*TVL*KHVGRVSTDL 354
>gb|CK124684.1|CK124684 BES1824109p11 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P119 5-PRIME, mRNA sequence
Length = 787
Score = 145 bits (311), Expect(2) = 2e-068
Identities = 57/114 (50%), Positives = 76/114 (66%)
Frame = +3 / +1
Query: 618 NLENPDLYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLNPP 797
NL + LYHY +FSDN++A SVV+NST +N+K PEK VFHLVTD++N+ M WF LN
Sbjct: 124 NLRDNSLYHYCVFSDNIIAVSVVINSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALNDY 303
Query: 798 GDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFKADRPKTLSAGSSNLKY 959
A + ++ V+DF WLN+SY PVLKQL+ AA + +YF + N KY
Sbjct: 304 RGAIVEIQKVEDFTWLNASYVPVLKQLQDAATQNFYFSGSGNRGTPIKFRNPKY 465
Score = 135 bits (289), Expect(2) = 2e-068
Identities = 50/68 (73%), Positives = 64/68 (94%)
Frame = +3 / +1
Query: 951 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVET 1130
+K+RNPKYLSMLNHLRFY+P++YP+L+K+LFLDDDIVVQ+DL+ L+ ++LNGNV GAVET
Sbjct: 442 IKFRNPKYLSMLNHLRFYIPEIYPELHKVLFLDDDIVVQKDLSDLFTINLNGNVMGAVET 621
Query: 1131 CGESFHRF 1154
C E+FHRF
Sbjct: 622 CMETFHRF 645
Score = 76.2 bits (160), Expect(2) = 9e-028
Identities = 41/86 (47%), Positives = 50/86 (58%)
Frame = -2 / -3
Query: 892 MAADSSCFRTGQ*EEFNHLKSSTFSTWIVASPGGFSKNHMFMAPKFSLSVTR*NTCFSGS 713
+AA SC RTG *E F+ +KSSTF +A F NH + + SVT+* T FSG
Sbjct: 398 VAASWSCLRTGT*EAFSQVKSSTFCISTMAPL*SFKANHALIGS*LTSSVTK*KTIFSGC 219
Query: 712 LAFMMVELTTTDAAKTLSEKRA*W*R 635
F +VEL TT+ A LSEK * *R
Sbjct: 218 FEFRVVELMTTETAMMLSEKTQ***R 141
Score = 68.0 bits (142), Expect(2) = 9e-028
Identities = 35/60 (58%), Positives = 38/60 (63%)
Frame = -2 / -3
Query: 1141 LSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMDKYFGF 962
+S VSTAP T PLR RS TT+SSSR L S G* G *NLR LS+DKYFGF
Sbjct: 632 VSMQVSTAPITLPLRLIVNKSDRSF*TTMSSSRNNTLCSSG*ISGM*NLRWLSIDKYFGF 453
>gb|BJ463761.1|BJ463761 BJ463761 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 5', mRNA sequence
Length = 596
Score = 133 bits (286), Expect(2) = 3e-063
Identities = 49/68 (72%), Positives = 64/68 (94%)
Frame = +3 / +3
Query: 951 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVET 1130
+K+RNPKYLSMLNHLRFY+P++YP+L+K++FLDDDIVVQ+DL+ L+ ++LNGNV GAVET
Sbjct: 297 IKFRNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVET 476
Query: 1131 CGESFHRF 1154
C E+FHRF
Sbjct: 477 CMETFHRF 500
Score = 129 bits (276), Expect(2) = 3e-063
Identities = 51/103 (49%), Positives = 69/103 (66%)
Frame = +3 / +3
Query: 651 LFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLNPPGDATIHVENVD 830
+FSDN++A SVV+NST +N+K PEK VFHLVTD++N+ M WF LN A + ++ V+
Sbjct: 12 VFSDNIIAVSVVINSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALNDYRGAIVEIQKVE 191
Query: 831 DFKWLNSSYCPVLKQLESAAMKEYYFKADRPKTLSAGSSNLKY 959
DF WLN+SY PVLKQL+ AA + +YF + N KY
Sbjct: 192 DFTWLNASYVPVLKQLQDAATQNFYFSGSGNRGTPIKFRNPKY 320
Score = 75.8 bits (159), Expect(2) = 2e-027
Identities = 38/80 (47%), Positives = 47/80 (58%)
Frame = -2 / -2
Query: 892 MAADSSCFRTGQ*EEFNHLKSSTFSTWIVASPGGFSKNHMFMAPKFSLSVTR*NTCFSGS 713
+AA SC RTG *E F+ +KSSTF +A F NH + + SVT+* T FSG
Sbjct: 253 VAASWSCLRTGT*EAFSQVKSSTFCISTMAPL*SFKANHALIGS*LTSSVTK*KTIFSGC 74
Query: 712 LAFMMVELTTTDAAKTLSEK 653
F +VEL TT+ A LSEK
Sbjct: 73 FEFRVVELMTTETAMMLSEK 14
Score = 67.5 bits (141), Expect(2) = 2e-027
Identities = 35/60 (58%), Positives = 38/60 (63%)
Frame = -2 / -2
Query: 1141 LSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMDKYFGF 962
+S VSTAP T PLR RS TT+SSSR L S G* G *NLR LS+DKYFGF
Sbjct: 487 VSMQVSTAPITLPLRLIVNKSDRSF*TTMSSSRNTTLCSSG*ISGM*NLRWLSIDKYFGF 308
>gb|BJ463762.1|BJ463762 BJ463762 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g14 5', mRNA sequence
Length = 500
Score = 133 bits (286), Expect(2) = 9e-063
Identities = 49/68 (72%), Positives = 64/68 (94%)
Frame = +3 / +2
Query: 951 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVET 1130
+K+RNPKYLSMLNHLRFY+P++YP+L+K++FLDDDIVVQ+DL+ L+ ++LNGNV GAVET
Sbjct: 296 IKFRNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVET 475
Query: 1131 CGESFHRF 1154
C E+FHRF
Sbjct: 476 CMETFHRF 499
Score = 127 bits (272), Expect(2) = 9e-063
Identities = 51/103 (49%), Positives = 68/103 (66%)
Frame = +3 / +2
Query: 651 LFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLNPPGDATIHVENVD 830
+FSDN++A SVV NST +N+K PEK VFHLVTD++N+ M WF LN A + ++ V+
Sbjct: 11 VFSDNIIAVSVVXNSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALNDYRGAIVEIQKVE 190
Query: 831 DFKWLNSSYCPVLKQLESAAMKEYYFKADRPKTLSAGSSNLKY 959
DF WLN+SY PVLKQL+ AA + +YF + N KY
Sbjct: 191 DFTWLNASYVPVLKQLQDAATQNFYFSGSGNRGTPIKFRNPKY 319
Score = 76.2 bits (160), Expect(2) = 1e-027
Identities = 38/80 (47%), Positives = 47/80 (58%)
Frame = -2 / -3
Query: 892 MAADSSCFRTGQ*EEFNHLKSSTFSTWIVASPGGFSKNHMFMAPKFSLSVTR*NTCFSGS 713
+AA SC RTG *E F+ +KSSTF +A F NH + + SVT+* T FSG
Sbjct: 252 VAASWSCLRTGT*EAFSQVKSSTFCISTMAPL*SFKANHALIGS*LTSSVTK*KTIFSGC 73
Query: 712 LAFMMVELTTTDAAKTLSEK 653
F +VEL TT+ A LSEK
Sbjct: 72 FEFRVVELXTTETAMMLSEK 13
Score = 67.5 bits (141), Expect(2) = 1e-027
Identities = 35/60 (58%), Positives = 38/60 (63%)
Frame = -2 / -3
Query: 1141 LSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMDKYFGF 962
+S VSTAP T PLR RS TT+SSSR L S G* G *NLR LS+DKYFGF
Sbjct: 486 VSMQVSTAPITLPLRLIVNKSDRSF*TTMSSSRNTTLCSSG*ISGM*NLRWLSIDKYFGF 307
>gb|BU968630.1|BU968630 HB08B19r BC Hordeum vulgare subsp. vulgare cDNA clone HB08B19
5-PRIME, mRNA sequence
Length = 521
Score = 231 bits (498), Expect = 8e-060
Identities = 92/101 (91%), Positives = 97/101 (96%)
Frame = +3 / +2
Query: 855 YCPVLKQLESAAMKEYYFKADRPKTLSAGSSNLKYRNPKYLSMLNHLRFYLPQVYPKLNK 1034
YCPVL+QLES AMKEYYFKADRP TLSAGSSNLKYRNPKYLSMLNHLRFYLPQ+YPKL+K
Sbjct: 23 YCPVLRQLESVAMKEYYFKADRPATLSAGSSNLKYRNPKYLSMLNHLRFYLPQIYPKLDK 202
Query: 1035 ILFLDDDIVVQRDLTGLWEVDLNGNVNGAVETCGESFHRFD 1157
ILFLDDDIVVQ+DLTGLW+VDLNG VNGAV TCGESFHRFD
Sbjct: 203 ILFLDDDIVVQKDLTGLWDVDLNGMVNGAVFTCGESFHRFD 325
Score = 150 bits (322), Expect = 2e-035
Identities = 68/100 (68%), Positives = 77/100 (77%)
Frame = -2 / -3
Query: 1156 SNR*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMD 977
S R KLSP V+TAP T P RSTS +PV+S TT+SSSRK IL + G* CGR*NL+ SM+
Sbjct: 324 SKRWKLSPQVNTAPLTIPFRSTSHNPVKSFCTTMSSSRKSILSNFG*ICGR*NLKWFSME 145
Query: 976 KYFGFRYFRLEEPAESVFGRSALK*YSFMAADSSCFRTGQ 857
+Y G RYFR EEPAE V GRSALK*YSFMA DSS +TGQ
Sbjct: 144 RYLGLRYFRFEEPAERVAGRSALK*YSFMATDSS*RKTGQ 25
Score = 111 bits (236), Expect = 1e-023
Identities = 50/101 (49%), Positives = 73/101 (72%)
Frame = -1 / -2
Query: 1157 IKSVKTLSTCFHGSIYISIKINLPESS*VPLDNYIIIQEKNFIQLGIDLWEVKS*MIEHG 978
+++V+TLST H +I SI+IN+P+S + L NY+II+EK IQL IDLWE+KS +++HG
Sbjct: 325 VETVETLSTGKHCTIDHSIQINIPQSCQIFLHNYVIIEEKYLIQLWIDLWEIKSQVVQHG 146
Query: 977 QIFWVSILQIRRTSRECFRTISLEIIFFHGCRLKLLQNRAV 855
++ V++ + RT + T+SLEII FHG RL+L NR V
Sbjct: 145 EVLGVAVF*V*RTC*KSGGTVSLEIILFHGNRLQLT*NRTV 23
>gb|BI950903.1|BI950903 HVSMEl0023J16f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0023J16f, mRNA sequence
Length = 536
Score = 211 bits (455), Expect = 7e-054
Identities = 85/173 (49%), Positives = 127/173 (73%)
Frame = +3 / +3
Query: 186 ADSRVRLMRDQMIMARIYSVLAKSRDKLDLYQELLARLKESQRSLGEATADAELPKSASD 365
+D+RVR +RDQ+I A++Y L R ++L R++E Q+ LG+A+ D++LPK+A++
Sbjct: 15 SDTRVRDIRDQLIKAKVYLGLGAIRANPQYLRDLRQRIREVQKVLGDASKDSDLPKNANE 194
Query: 366 RTKAMGQVLSKARDLLYDCKEITQRLRAMLQSADEQVRSLKKQSTFLSQLAAKTIPNGIH 545
+ KA+ Q L K + DC ++LRAML SA+EQ+ + KKQ+ FL+QLAAKT+P G+H
Sbjct: 195 KVKALEQTLVKGKQTQDDCSVAVKKLRAMLHSAEEQLHAQKKQTVFLTQLAAKTLPKGLH 374
Query: 546 CLSMRLTIDYYLLSPEKRKFPNSENLENPDLYHYALFSDNVLAASVVVNSTIM 704
CL +RL +Y+ L P +++FPN E L++P LYHYALFSDN+LA +VVVNST++
Sbjct: 375 CLPLRLANEYFSLDPSQQQFPNHEKLDDPKLYHYALFSDNILATAVVVNSTVL 533
>gb|CK122439.1|CK122439 BES1824103h01 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010H013 5-PRIME, mRNA sequence
Length = 653
Score = 80.3 bits (169), Expect(3) = 2e-040
Identities = 37/55 (67%), Positives = 38/55 (69%)
Frame = -2 / -3
Query: 178 FSFSRLQMLYFPSTAESTAFERSIPAFSSLTNASSSCCXTSIFFLTATLLNFMCV 14
F F LYFPSTAESTAFERSIPAFSSLT ASSSCC SIF NF+ V
Sbjct: 456 FHFHVSIKLYFPSTAESTAFERSIPAFSSLTRASSSCCTVSIFITPVLSPNFLRV 292
Score = 78.0 bits (164), Expect(3) = 2e-048
Identities = 32/35 (91%), Positives = 34/35 (97%)
Frame = +3 / +3
Query: 156 IWRRENENEKADSRVRLMRDQMIMARIYSVLAKSR 260
+WRRENENEKAD+ VRLMRDQMIMARIYSVLAKSR
Sbjct: 435 LWRRENENEKADANVRLMRDQMIMARIYSVLAKSR 539
Score = 77.6 bits (163), Expect(3) = 2e-048
Identities = 33/36 (91%), Positives = 36/36 (100%)
Frame = +3 / +1
Query: 261 DKLDLYQELLARLKESQRSLGEATADAELPKSASDR 368
DKLDLY+ELLAR+KESQRSLGEATADAELPKSAS+R
Sbjct: 541 DKLDLYRELLARIKESQRSLGEATADAELPKSASER 648
Score = 76.2 bits (160), Expect(3) = 2e-048
Identities = 36/48 (75%), Positives = 37/48 (77%)
Frame = +3 / +2
Query: 12 ITHMKLRRVAVRKKMEVXQQDDEALVKLENAGIERSKAVDSAVLGKYS 155
+T KL KME QQDDEALVKLENAGIERSKAVDSAVLGKYS
Sbjct: 290 VTRRKLGERTGVIKMETVQQDDEALVKLENAGIERSKAVDSAVLGKYS 433
Score = 71.6 bits (150), Expect(2) = 3e-022
Identities = 28/34 (82%), Positives = 30/34 (88%)
Frame = +2 / +3
Query: 260 GQA*SLSGAACKAQGKPAIPWGSYC*C*TSQECF 361
GQA SLSGA C+ QGKPA+PWGSYC*C*TS ECF
Sbjct: 540 GQARSLSGATCQDQGKPALPWGSYC*C*TS*ECF 641
Score = 68.0 bits (142), Expect(3) = 2e-040
Identities = 27/36 (75%), Positives = 31/36 (86%)
Frame = -3 / -2
Query: 366 YPKHSWEVQHQQ*LPQGIAGFP*ALQAAPDRDQACP 259
+ KHS EVQHQQ*LPQG AGFP*+ Q APD+D+ACP
Sbjct: 646 FQKHS*EVQHQQ*LPQGSAGFP*SWQVAPDKDRACP 539
Score = 61.1 bits (127), Expect(3) = 9e-032
Identities = 28/37 (75%), Positives = 32/37 (86%)
Frame = -1 / -3
Query: 368 SIRSTLGKFSISSSFPKGSLAFLEPCKQLLIEIKLVP 258
S RSTL KFSISSSFPKG+LAFL+P K L I+I+LVP
Sbjct: 648 SFRSTLRKFSISSSFPKGALAFLDPGK*LPIKIELVP 538
Score = 60.2 bits (125), Expect(3) = 9e-032
Identities = 27/47 (57%), Positives = 30/47 (63%)
Frame = -1 / -2
Query: 152 VFSQHSRVNSF*TFDTCILKFN*RFIILLXYFHFLPYSNSP*FHVCY 12
+FSQHSR+N *T +TCILKFN* FIILL FH P F Y
Sbjct: 430 IFSQHSRINCL*TLNTCILKFN*GFIILLHRFHLYHTGPLPQFPASY 290
Score = 56.5 bits (117), Expect(3) = 2e-040
Identities = 22/36 (61%), Positives = 27/36 (75%)
Frame = -1 / -1
Query: 266 LVPRFGKNRIYSGHDHLISHQTDP*ICLFIFIFTSP 159
L P F K+ +YSGHDHL+ HQT+ I L +FIFTSP
Sbjct: 545 LSPGFSKHGVYSGHDHLVPHQTNICIRLLVFIFTSP 438
Score = 54.2 bits (112), Expect(2) = 3e-022
Identities = 27/46 (58%), Positives = 32/46 (69%)
Frame = +2 / +1
Query: 14 NTHEIKESCCKEENGSXAAG**SVS*T*ECRYRTFKSC*LCCAGKI 151
N+ EI +++G+ AAG**S S*T*ECRY FK * CCAGKI
Sbjct: 292 NSQEIGGEDRCDKDGNGAAG**SPS*T*ECRY*AFKGS*FCCAGKI 429
Score = 54.2 bits (112), Expect(3) = 9e-032
Identities = 25/33 (75%), Positives = 26/33 (78%)
Frame = -2 / -2
Query: 244 TEYILAMII*SRIKRTLESAFSFSFSRLQMLYF 146
TEYILAMII SRI+RT SAFSFSFSRL F
Sbjct: 523 TEYILAMIIWSRIRRTFASAFSFSFSRLHKTIF 425
>gb|CA018769.1|CA018769 HV09J15r HV Hordeum vulgare subsp. vulgare cDNA clone HV09J15
5-PRIME, mRNA sequence
Length = 586
Score = 122 bits (262), Expect(2) = 7e-047
Identities = 45/75 (60%), Positives = 61/75 (81%)
Frame = +3 / +2
Query: 930 LSAGSSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGN 1109
L +K+ NPK++S+LNHLRFY+PQ+ P L K++FLDDD+VVQ+DLT L+ ++L+GN
Sbjct: 239 LKTQEREIKFHNPKFVSLLNHLRFYIPQILPNLEKVVFLDDDVVVQKDLTQLFSIELHGN 418
Query: 1110 VNGAVETCGESFHRF 1154
V GAVETC ESFHR+
Sbjct: 419 VIGAVETCLESFHRY 463
Score = 85.4 bits (180), Expect(2) = 7e-047
Identities = 31/71 (43%), Positives = 48/71 (67%)
Frame = +3 / +2
Query: 684 VVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLNPPGDATIHVENVDDFKWLNSSYCP 863
VVNST+ NA P++ VFH+VTD++NFGAM+ WFL+N T+ V +D+F W N++
Sbjct: 11 VVNSTVSNANHPQQLVFHVVTDRINFGAMSTWFLINDFKGCTVEVHCIDEFSWFNATASS 190
Query: 864 VLKQLESAAMK 896
++++L K
Sbjct: 191 LVRRLSDMETK 223
>gb|BQ759105.1|BQ759105 EBma07_SQ003_E23_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ003_E23 5', mRNA sequence
Length = 554
Score = 164 bits (353), Expect(2) = 5e-044
Identities = 67/161 (41%), Positives = 106/161 (65%)
Frame = +3 / +1
Query: 189 DSRVRLMRDQMIMARIYSVLAKSRDKLDLYQELLARLKESQRSLGEATADAELPKSASDR 368
D+ +R+++DQ+I A+ Y + SR +EL AR+K+ Q +LG+AT D LP++ +
Sbjct: 22 DATIRIIKDQLIRAKTYLGVLASRGNHGTAKELRARMKDIQIALGDATDDGMLPQNVHGK 201
Query: 369 TKAMGQVLSKARDLLYDCKEITQRLRAMLQSADEQVRSLKKQSTFLSQLAAKTIPNGIHC 548
KAM Q L + + + C RLR L S +E+++S +K + +L+QLAAK++P G+HC
Sbjct: 202 IKAMEQTLGRIKRMHDGCSSAVNRLRTSLHSTEERLQSHRKDANYLAQLAAKSLPKGLHC 381
Query: 549 LSMRLTIDYYLLSPEKRKFPNSENLENPDLYHYALFSDNVL 671
L +RLT +YYL + + FPN+E LE+P L+HYA+FSD +
Sbjct: 382 LPLRLTNEYYLSNSNNKDFPNTEKLEDPKLHHYAVFSDKCI 504
Score = 34.1 bits (68), Expect(2) = 5e-044
Identities = 14/19 (73%), Positives = 18/19 (94%)
Frame = +3 / +2
Query: 663 NVLAASVVVNSTIMNAKEP 719
NVLAA+VVVNST++ AK+P
Sbjct: 497 NVLAAAVVVNSTLVYAKKP 553
>gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006H13f, mRNA sequence
Length = 1018
Score = 149 bits (320), Expect(2) = 1e-042
Identities = 54/70 (77%), Positives = 66/70 (94%)
Frame = +3 / +1
Query: 945 SNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAV 1124
S+LKYRNPKYLSMLNHLRFYLP++YPKL+K++FLD D+VV++D+TGLW +D+ G VNGAV
Sbjct: 106 SDLKYRNPKYLSMLNHLRFYLPEIYPKLDKMVFLDGDVVVRKDITGLWSIDMKGKVNGAV 285
Query: 1125 ETCGESFHRF 1154
ETCGESFHRF
Sbjct: 286 ETCGESFHRF 315
Score = 93.6 bits (198), Expect(2) = 1e-022
Identities = 44/69 (63%), Positives = 49/69 (71%)
Frame = -2 / -3
Query: 1150 R*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMDKY 971
R KLSP VSTAP TFP S Q PV S TT S SRK IL S G* G+*NLR*LS+++Y
Sbjct: 311 RWKLSPQVSTAPLTFPFISIDQRPVMSFLTTTSPSRKTILSSFG*ISGK*NLR*LSIERY 132
Query: 970 FGFRYFRLE 944
FGF YF+ E
Sbjct: 131 FGFLYFKSE 105
Score = 44.6 bits (91), Expect(2) = 1e-042
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3 / +1
Query: 828 DDFKWLNSSYCPVLKQLESAAMKEYYFKADR 920
D+F WLN S VLKQL S +M +YYF+A R
Sbjct: 1 DEFTWLNDSSSLVLKQLGSQSMIDYYFRAQR 93
Score = 33.1 bits (66), Expect(2) = 1e-022
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = -2 / -3
Query: 937 AESVFGRSALK*YSFMAADSSCFRTGQ*EEFNHLKS 830
+ES F R ALK* S + D +CF T FNH+ S
Sbjct: 110 SESEFARCALK**SIID*DPNCFSTRLELSFNHVNS 3
>gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012C17f, mRNA sequence
Length = 785
Score = 149 bits (320), Expect(2) = 1e-042
Identities = 54/70 (77%), Positives = 66/70 (94%)
Frame = +3 / +1
Query: 945 SNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAV 1124
S+LKYRNPKYLSMLNHLRFYLP++YPKL+K++FLD D+VV++D+TGLW +D+ G VNGAV
Sbjct: 106 SDLKYRNPKYLSMLNHLRFYLPEIYPKLDKMVFLDGDVVVRKDITGLWSIDMKGKVNGAV 285
Query: 1125 ETCGESFHRF 1154
ETCGESFHRF
Sbjct: 286 ETCGESFHRF 315
Score = 93.6 bits (198), Expect(2) = 1e-022
Identities = 44/69 (63%), Positives = 49/69 (71%)
Frame = -2 / -1
Query: 1150 R*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMDKY 971
R KLSP VSTAP TFP S Q PV S TT S SRK IL S G* G+*NLR*LS+++Y
Sbjct: 311 RWKLSPQVSTAPLTFPFISIDQRPVMSFLTTTSPSRKTILSSFG*ISGK*NLR*LSIERY 132
Query: 970 FGFRYFRLE 944
FGF YF+ E
Sbjct: 131 FGFLYFKSE 105
Score = 44.6 bits (91), Expect(2) = 1e-042
Identities = 18/31 (58%), Positives = 22/31 (70%)
Frame = +3 / +1
Query: 828 DDFKWLNSSYCPVLKQLESAAMKEYYFKADR 920
D+F WLN S VLKQL S +M +YYF+A R
Sbjct: 1 DEFTWLNDSSSLVLKQLGSQSMIDYYFRAQR 93
Score = 33.1 bits (66), Expect(2) = 1e-022
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = -2 / -1
Query: 937 AESVFGRSALK*YSFMAADSSCFRTGQ*EEFNHLKS 830
+ES F R ALK* S + D +CF T FNH+ S
Sbjct: 110 SESEFARCALK**SIID*DPNCFSTRLELSFNHVNS 3
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 170 bits (365), Expect = 2e-041
Identities = 67/73 (91%), Positives = 71/73 (97%)
Frame = +3 / +2
Query: 939 GSSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNG 1118
GSSNLKYRNPKYLSMLNHLRFYLPQ+YPKL+KILFLDDDIVVQ+DLTGLW+VDLNG VNG
Sbjct: 14 GSSNLKYRNPKYLSMLNHLRFYLPQIYPKLDKILFLDDDIVVQKDLTGLWDVDLNGMVNG 193
Query: 1119 AVETCGESFHRFD 1157
AV TCGESFHRFD
Sbjct: 194 AVFTCGESFHRFD 232
Score = 109 bits (232), Expect = 4e-023
Identities = 47/73 (64%), Positives = 55/73 (75%)
Frame = -2 / -2
Query: 1156 SNR*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMD 977
S R KLSP V+TAP T P RSTS +PV+S TT+SSSRK IL + G* CGR*NL+ SM+
Sbjct: 231 SKRWKLSPQVNTAPLTIPFRSTSHNPVKSFCTTMSSSRKSILSNFG*ICGR*NLKWFSME 52
Query: 976 KYFGFRYFRLEEP 938
+Y G RYFR EEP
Sbjct: 51 RYLGLRYFRFEEP 13
>gb|BU995595.1|BU995595 HM10M02r HM Hordeum vulgare subsp. vulgare cDNA clone HM10M02
5-PRIME, mRNA sequence
Length = 657
Score = 133 bits (285), Expect(2) = 3e-039
Identities = 55/102 (53%), Positives = 76/102 (74%)
Frame = +3 / +1
Query: 606 PNSENLENPDLYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFL 785
P LE+P L+HYA+FSDNVLAASVVV S + N+++P KHVFH+VTD++N GAM +
Sbjct: 277 PPPAALEDPALFHYAIFSDNVLAASVVVRSCVANSQDPSKHVFHVVTDRMNLGAMQVIIR 456
Query: 786 LNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFK 911
L A V+ +D+K+LNSSY PVL+QLESA ++++YF+
Sbjct: 457 LMDLQGAHYEVKAYEDYKFLNSSYVPVLRQLESANLQKFYFE 582
Score = 49.2 bits (101), Expect(2) = 3e-039
Identities = 21/54 (38%), Positives = 35/54 (64%)
Frame = +3 / +1
Query: 411 LYDCKEITQRLRAMLQSADEQVRSLKKQSTFLSQLAAKTIPNGIHCLSMRLTID 572
L+D + QRL+ + + +E + KK+ F S +AAK+IP +HCL++RLT +
Sbjct: 79 LFDPQLKIQRLKDAIFAQNELLARAKKRGAFASLIAAKSIPKPLHCLAVRLTAE 240
>gb|BF266558.3|BF266558 HV_CEa0015H07f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0015H07f, mRNA sequence
Length = 767
Score = 149 bits (321), Expect = 2e-035
Identities = 57/89 (64%), Positives = 75/89 (84%)
Frame = +3 / +3
Query: 885 AAMKEYYFKADRPKTLSAGSSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVV 1064
+A + ++ ++ + + +SN+K+RNPKYLSMLNHLRFYLP++YPKL KILFLDDD+VV
Sbjct: 333 SANLQKFYFENKLENATKDASNMKFRNPKYLSMLNHLRFYLPEMYPKLQKILFLDDDVVV 512
Query: 1065 QRDLTGLWEVDLNGNVNGAVETCGESFHR 1151
QRDLTGLW++D++G VNGAVETC SFHR
Sbjct: 513 QRDLTGLWKIDMDGKVNGAVETCFGSFHR 599
Score = 133 bits (285), Expect(2) = 5e-038
Identities = 55/102 (53%), Positives = 76/102 (74%)
Frame = +3 / +3
Query: 606 PNSENLENPDLYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFL 785
P LE+P L+HYA+FSDNVLAASVVV S + N+++P KHVFH+VTD++N GAM +
Sbjct: 57 PPPAALEDPALFHYAIFSDNVLAASVVVRSCVANSQDPSKHVFHVVTDRMNLGAMQVIIR 236
Query: 786 LNPPGDATIHVENVDDFKWLNSSYCPVLKQLESAAMKEYYFK 911
L A V+ +D+K+LNSSY PVL+QLESA ++++YF+
Sbjct: 237 LMDLQGAHYEVKAYEDYKFLNSSYVPVLRQLESANLQKFYFE 362
Score = 80.3 bits (169), Expect(3) = 3e-029
Identities = 40/69 (57%), Positives = 47/69 (68%)
Frame = -2 / -2
Query: 1153 NR*KLSPHVSTAPFTFPLRSTSQSPVRSLWTTISSSRKRILFSLG*TCGR*NLR*LSMDK 974
+R* HVSTAPFTFP S +PVRS TT SSSRKRI SLG G+*N +*LS+++
Sbjct: 601 SR*NDPKHVSTAPFTFPSMSIFHNPVRSRCTTTSSSRKRIFCSLGYISGK*NRK*LSIER 422
Query: 973 YFGFRYFRL 947
Y GF F L
Sbjct: 421 YLGFLNFIL 395
Score = 57.0 bits (118), Expect(3) = 3e-029
Identities = 27/49 (55%), Positives = 31/49 (63%)
Frame = -2 / -2
Query: 769 MAPKFSLSVTR*NTCFSGSLAFMMVELTTTDAAKTLSEKRA*W*RSGFS 623
+AP+F SVT TCF GS E TTT+AA TLSEK A*W +G S
Sbjct: 220 IAPRFIRSVTTWKTCFEGSCELATQERTTTEAASTLSEKMA*WKSAGSS 74
Score = 45.1 bits (92), Expect(2) = 5e-038
Identities = 29/66 (43%), Positives = 34/66 (51%)
Frame = +1 / +1
Query: 934 LLVLLI*SIETQNICPCSII*DFTSHKSIPS*IKFFSWMMI*LSRGT*LDSGRLILMEM* 1113
L +L I*S +I C I D T + PS +FFSWMM+ G D GRL M *
Sbjct: 382 LKMLAI*SSGIPSIFLCLITCDSTCLRCTPSCRRFFSWMMMSSYSGISQDCGRLTWMGR* 561
Query: 1114 MEPWKH 1131
ME KH
Sbjct: 562 MEQ*KH 579
Score = 29.9 bits (59), Expect(3) = 3e-029
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = -2 / -2
Query: 886 ADSSCFRTGQ*EEFNHLKSS 827
ADSSC TG *EEF +L SS
Sbjct: 337 ADSSCRSTGT*EEFRNL*SS 278
>gb|BI777829.2|BI777829 EBro08_SQ002_D17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ002_D17 5', mRNA sequence
Length = 562
Score = 122 bits (262), Expect(2) = 1e-037
Identities = 45/75 (60%), Positives = 61/75 (81%)
Frame = +3 / +2
Query: 930 LSAGSSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGN 1109
L +K+ NPK++S+LNHLRFY+PQ+ P L K++FLDDD+VVQ+DLT L+ ++L+GN
Sbjct: 170 LKTQEREIKFHNPKFVSLLNHLRFYIPQILPNLEKVVFLDDDVVVQKDLTQLFSIELHGN 349
Query: 1110 VNGAVETCGESFHRF 1154
V GAVETC ESFHR+
Sbjct: 350 VIGAVETCLESFHRY 394
Score = 54.7 bits (113), Expect(2) = 1e-037
Identities = 18/45 (40%), Positives = 31/45 (68%)
Frame = +3 / +2
Query: 744 TDKLNFGAMNMWFLLNPPGDATIHVENVDDFKWLNSSYCPVLKQL 878
TD++NFGAM+ WFL+N T+ V +D+F W N++ ++++L
Sbjct: 2 TDRINFGAMSTWFLINDFKGCTVEVHCIDEFSWFNATASSLVRRL 136
>gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 5', mRNA sequence
Length = 606
Score = 151 bits (324), Expect = 8e-036
Identities = 57/90 (63%), Positives = 76/90 (84%)
Frame = +3 / +1
Query: 885 AAMKEYYFKADRPKTLSAGSSNLKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVV 1064
+A + ++ ++ + + +SN+K+RNPKYLSMLNHLRFYLP++YPKL KILFLDDD+VV
Sbjct: 127 SANLQKFYFENKLENATKDASNMKFRNPKYLSMLNHLRFYLPEMYPKLQKILFLDDDVVV 306
Query: 1065 QRDLTGLWEVDLNGNVNGAVETCGESFHRF 1154
QRDLTGLW++D++G VNGAVETC SFHR+
Sbjct: 307 QRDLTGLWKIDMDGKVNGAVETCFGSFHRY 396
>gb|AV836570.1|AV836570 AV836570 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd25o11, mRNA
sequence
Length = 423
Score = 135 bits (289), Expect = 5e-031
Identities = 56/124 (45%), Positives = 87/124 (70%)
Frame = +3 / +2
Query: 186 ADSRVRLMRDQMIMARIYSVLAKSRDKLDLYQELLARLKESQRSLGEATADAELPKSASD 365
+D+RVR +RDQ+I A++Y L R ++L R++E Q+ LG+A+ D++LPK+A++
Sbjct: 50 SDTRVRDIRDQLIKAKVYLGLGAIRANPQYLRDLRQRIREVQKVLGDASKDSDLPKNANE 229
Query: 366 RTKAMGQVLSKARDLLYDCKEITQRLRAMLQSADEQVRSLKKQSTFLSQLAAKTIPNGIH 545
+ KA+ Q L K + DC ++LRAML SA+EQ+ + KKQ+ FL+QLAAKT+P G+H
Sbjct: 230 KVKALEQTLVKGKQTQDDCSVAVKKLRAMLHSAEEQLHAQKKQTVFLTQLAAKTLPKGLH 409
Query: 546 CLSM 557
CL +
Sbjct: 410 CLPL 421
>gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day germination, no treatment, cv Optic,
EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_D08 5', mRNA sequence
Length = 464
Score = 133 bits (286), Expect = 1e-030
Identities = 49/68 (72%), Positives = 64/68 (94%)
Frame = +3 / +3
Query: 951 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVET 1130
+K+RNPKYLSMLNHLRFY+P++YP+L+K++FLDDDIVVQ+DL+ L+ ++LNGNV GAVET
Sbjct: 27 IKFRNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVET 206
Query: 1131 CGESFHRF 1154
C E+FHRF
Sbjct: 207 CMETFHRF 230
>gb|BM444949.1|BM444949 EBem09_SQ006_P20_R embryo, 1 Day germination, no treatment, cv Optic,
EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ006_P20 5', mRNA sequence
Length = 417
Score = 133 bits (286), Expect = 1e-030
Identities = 49/68 (72%), Positives = 64/68 (94%)
Frame = +3 / +3
Query: 951 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVET 1130
+K+RNPKYLSMLNHLRFY+P++YP+L+K++FLDDDIVVQ+DL+ L+ ++LNGNV GAVET
Sbjct: 27 IKFRNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVET 206
Query: 1131 CGESFHRF 1154
C E+FHRF
Sbjct: 207 CMETFHRF 230
>gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day germination, no treatment, cv Optic,
EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ001_M07 5', mRNA sequence
Length = 512
Score = 133 bits (286), Expect = 1e-030
Identities = 49/68 (72%), Positives = 64/68 (94%)
Frame = +3 / +3
Query: 951 LKYRNPKYLSMLNHLRFYLPQVYPKLNKILFLDDDIVVQRDLTGLWEVDLNGNVNGAVET 1130
+K+RNPKYLSMLNHLRFY+P++YP+L+K++FLDDDIVVQ+DL+ L+ ++LNGNV GAVET
Sbjct: 27 IKFRNPKYLSMLNHLRFYIPEIYPELHKVVFLDDDIVVQKDLSDLFTINLNGNVMGAVET 206
Query: 1131 CGESFHRF 1154
C E+FHRF
Sbjct: 207 CMETFHRF 230
>gb|BU988833.1|BU988833 HF18N17r HF Hordeum vulgare subsp. vulgare cDNA clone HF18N17
5-PRIME, mRNA sequence
Length = 610
Score = 94.5 bits (200), Expect(2) = 1e-028
Identities = 38/75 (50%), Positives = 52/75 (69%)
Frame = +3 / +1
Query: 621 LENPDLYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLNPPG 800
L + +H+ L SDN+LAASVVV ST+ +A +PE+ VFH++TDK + AM+ WF LNP
Sbjct: 379 LSDNSYFHFVLASDNILAASVVVRSTVRSALKPERIVFHVITDKKTYPAMHSWFALNPLY 558
Query: 801 DATIHVENVDDFKWL 845
A I V+ V F+WL
Sbjct: 559 PAIIEVKGVHQFEWL 603
Score = 52.8 bits (109), Expect(2) = 1e-028
Identities = 23/68 (33%), Positives = 38/68 (55%)
Frame = +3 / +1
Query: 414 YDCKEITQRLRAMLQSADEQVRSLKKQSTFLSQLAAKTIPNGIHCLSMRLTIDYYLLSPE 593
YD K RL+A +++ D+++RS + AA IP G++CLS+RLT Y +
Sbjct: 163 YDLKSFALRLKATMETMDKELRSSRLSEQLNKHYAAIAIPKGLYCLSLRLTDVYSSNALA 342
Query: 594 KRKFPNSE 617
+++ P E
Sbjct: 343 RKQLPPPE 366
>gb|CA012566.1|CA012566 HT05M19r HT Hordeum vulgare subsp. vulgare cDNA clone HT05M19
5-PRIME, mRNA sequence
Length = 352
Score = 100 bits (213), Expect(2) = 1e-028
Identities = 38/80 (47%), Positives = 56/80 (70%)
Frame = +3 / +1
Query: 657 SDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLNPPGDATIHVENVDDF 836
+DNVLA SVVVNST+ NA P++ VFH+VTD++NFGAM+ WFL+N T+ V +D+F
Sbjct: 10 ADNVLATSVVVNSTVSNANHPQQLVFHVVTDRINFGAMSTWFLINDFKGCTVEVHCIDEF 189
Query: 837 KWLNSSYCPVLKQLESAAMK 896
W N++ ++++L K
Sbjct: 190 SWFNATASSLVRRLSDMETK 249
Score = 46.9 bits (96), Expect(2) = 1e-028
Identities = 15/29 (51%), Positives = 22/29 (75%)
Frame = +3 / +1
Query: 930 LSAGSSNLKYRNPKYLSMLNHLRFYLPQV 1016
L +K+ NPK++S+LNHLRFY+PQ+
Sbjct: 265 LKTQEREIKFHNPKFVSLLNHLRFYIPQI 351
>gb|AL450785.1|AL450785 AL450785 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK04D14u 5', mRNA sequence
Length = 296
Score = 90.0 bits (190), Expect(2) = 4e-024
Identities = 34/58 (58%), Positives = 43/58 (74%)
Frame = +3 / +3
Query: 618 NLENPDLYHYALFSDNVLAASVVVNSTIMNAKEPEKHVFHLVTDKLNFGAMNMWFLLN 791
NL + LYHY +FS N+ A SVV+NST +N+K PEK VFHLVTD++N+ M WF LN
Sbjct: 9 NLRDNSLYHYCVFSXNIXAVSVVINSTTLNSKHPEKIVFHLVTDEVNYDPMRAWFALN 182
Score = 41.8 bits (85), Expect(2) = 4e-024
Identities = 16/24 (66%), Positives = 18/24 (75%)
Frame = +3 / +2
Query: 831 DFKWLNSSYCPVLKQLESAAMKEY 902
DF WLN+SY PVLKQL AA E+
Sbjct: 224 DFTWLNASYVPVLKQLXDAAYTEF 295
>gb|BE455944.3|BE455944 HVSMEg0017N04f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017N04f, mRNA sequence
Length = 805
Score = 109 bits (232), Expect = 4e-023
Identities = 47/116 (40%), Positives = 75/116 (64%)
Frame = +3 / +3
Query: 186 ADSRVRLMRDQMIMARIYSVLAKSRDKLDLYQELLARLKESQRSLGEATADAELPKSASD 365
+D+RVR +RDQ+I A++Y L R ++L R++E Q+ LG+A+ D++LPK+A++
Sbjct: 405 SDTRVRDIRDQLIKAKVYLGLGAIRANPQYLRDLRQRIREVQKVLGDASKDSDLPKNANE 584
Query: 366 RTKAMGQVLSKARDLLYDCKEITQRLRAMLQSADEQVRSLKKQSTFLSQLAAKTIP 533
+ KA+ Q L K + DC ++LRAML SA+EQ+ + K+Q+ FL L K P
Sbjct: 585 KVKALEQTLVKGKQTQDDCSXAVKKLRAMLHSAEEQLHAQKRQTVFLHNLQPKPFP 752
>gb|AV836656.1|AV836656 AV836656 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd23f10, mRNA
sequence
Length = 622
Score = 105 bits (224), Expect = 5e-022
Identities = 43/57 (75%), Positives = 52/57 (91%)
Frame = +3 / +1
Query: 45 RKKMEVXQQDDEALVKLENAGIERSKAVDSAVLGKYSIWRRENENEKADSRVRLMRD 215
++ +++ ++DDEALVKLENA IERSKAVDSAVLGKYSIWR+ENENE +DS VRLMRD
Sbjct: 451 KRAIDLVRKDDEALVKLENAAIERSKAVDSAVLGKYSIWRKENENENSDSTVRLMRD 621
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 506,590,463
Number of Sequences: 312970
Number of extensions: 7148522
Number of successful extensions: 32942
Number of sequences better than 1.0e-020: 37
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 14584978381
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)