TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115208.2.1
(1469 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442103.2|BM442103 EBan01_SQ002_D11_R anther, yellow st... 261 e-104
gb|BU996673.1|BU996673 HM14F23r HM Hordeum vulgare subsp. v... 165 4e-082
gb|CV055163.1|CV055163 BNEL117e7 Barley EST endosperm libra... 160 9e-054
gb|BM442520.2|BM442520 EBan01_SQ003_G14_R anther, yellow st... 204 7e-052
gb|BE420880.1|BE420880 HWM003.E05 ITEC HWM Barley Leaf Libr... 114 5e-045
gb|CV063350.1|CV063350 BNEL89d11 Barley EST endosperm libra... 160 3e-044
gb|CK125352.1|CK125352 BES1824107m07 BES1824 Hordeum vulgar... 133 2e-043
gb|CV058428.1|CV058428 BNEL37h7 Barley EST endosperm librar... 160 7e-041
gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots... 138 1e-038
gb|CV059576.1|CV059576 BNEL49f3 Barley EST endosperm librar... 157 1e-037
gb|BF253711.2|BF253711 HVSMEf0001O16f Hordeum vulgare seedl... 108 1e-035
gb|AV836267.1|AV836267 AV836267 K. Sato unpublished cDNA li... 77 6e-034
gb|CV062756.1|CV062756 BNEL82f4 Barley EST endosperm librar... 144 2e-033
gb|AL507518.1|AL507518 AL507518 Hordeum vulgare Barke devel... 72 2e-032
gb|AJ461534.1|AJ461534 AJ461534 S00002 Hordeum vulgare subs... 126 4e-028
gb|CB880252.1|CB880252 HM05P11w HM Hordeum vulgare subsp. v... 122 5e-027
gb|AJ462026.1|AJ462026 AJ462026 S00002 Hordeum vulgare subs... 110 2e-023
>gb|BM442103.2|BM442103 EBan01_SQ002_D11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_D11 5', mRNA sequence
Length = 676
Score = 261 bits (565), Expect(2) = e-104
Identities = 102/145 (70%), Positives = 118/145 (81%)
Frame = +3 / +2
Query: 297 SGAPPAPNMTEPPAGWAWSINQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSR 476
S +PP+P + E PAGWAWSINQ RSFRWNLTASAARPNPQGSYHYGQINITRTIK+ SR
Sbjct: 242 SNSPPSPKLPEAPAGWAWSINQWRSFRWNLTASAARPNPQGSYHYGQINITRTIKLSTSR 421
Query: 477 GHIDGKLRYGFNGISHRDPETPVKLAEYFNATDGVFSYNQMGDVPPAVNGPLHVIPNVIT 656
G +DGK RY NG+SH D ETP+KLAEYFNAT GVF YN + D PP + + PNVIT
Sbjct: 422 GKVDGKERYALNGVSHADAETPLKLAEYFNATKGVFEYNLITDTPPKEGTLIKLAPNVIT 601
Query: 657 AEFRTFIEIVFENPEKSIDSLHVDG 731
AE RT+IE+VFENPE+SID+ H++G
Sbjct: 602 AEHRTYIEVVFENPERSIDTFHLNG 676
Score = 197 bits (424), Expect(2) = 1e-074
Identities = 92/142 (64%), Positives = 99/142 (69%)
Frame = -2 / -2
Query: 730 PSTWRESMLFSGFSKTISMKVRNSAVMTLGMTWRGPFTAGGTSPIWL*LNTPSVALKYSA 551
P W+ SML SGFSKT SM VR SAVMTLG + + GG S + L NTP VALKYSA
Sbjct: 675 PLRWKVSMLLSGFSKTTSM*VRCSAVMTLGASLMSVPSLGGVSVMRLYSNTPLVALKYSA 496
Query: 550 SFTGVSGSLWEMPLKP*RSLPSMWPRETMTLMVRVMLIWP*W*EPCGLGRAALAVRFQRN 371
S GVS S WE PL+ SLPS PR +LMVRVMLIWP*W*+PCGLG AALAVRFQR
Sbjct: 495 SLRGVSASAWETPLRAYLSLPSTLPRLVESLMVRVMLIWP*W*DPCGLGLAALAVRFQRK 316
Query: 370 DLAWLMDQAQPAGGSVMFGAGG 305
DL WLMD A PAG S G GG
Sbjct: 315 DLHWLMDHAHPAGASGSLGDGG 250
Score = 137 bits (293), Expect(2) = e-104
Identities = 53/85 (62%), Positives = 67/85 (78%)
Frame = +3 / +2
Query: 54 CNTGIKASLNFRIQGHDMKLVELEGSHTLQDVYDSLDVHVGHCLSVLVDADQAPGDYYMV 233
CN GIK +LN RIQGH +KLVE+EGSHT+Q+ YDSLDVH+G CLS L A+Q PGDY+++
Sbjct: 2 CNVGIKTTLNVRIQGHVVKLVEMEGSHTVQNEYDSLDVHIGQCLSFLTTANQKPGDYFLI 181
Query: 234 ASTRFIHDAKSASAVIRYAGSSGAP 308
ASTRFI + +AV+RY GS+ P
Sbjct: 182 ASTRFIKGVNTITAVMRYKGSNSPP 256
Score = 104 bits (221), Expect(2) = 1e-074
Identities = 48/84 (57%), Positives = 51/84 (60%)
Frame = -2 / -2
Query: 307 GAPLEPA*RMTAEADLASWMNRVEATM**SPGAWSASTSTERQWPTCTSSESYTSCRVCE 128
G LEP *RMTA L M RVEA *SPG W A ER WP TSSESY+ C V E
Sbjct: 255 GGELEPL*RMTAVMVLTPLMKRVEAMRK*SPGFWLAVVRKERHWPMWTSSESYSFCTVWE 76
Query: 127 PSSSTSFMSWPWMRKLSDALIPVL 56
PS STS +WPWMR L L+P L
Sbjct: 75 PSISTSLTTWPWMRTLRVVLMPTL 4
Score = 64.3 bits (134), Expect(3) = 8e-026
Identities = 24/31 (77%), Positives = 29/31 (93%)
Frame = +2 / +1
Query: 338 RLGLVHQPGQVVPLEPDGQRRAAQPAGLLPL 430
R+G+VHQP +V+PLEPDGQRR AQPAG+LPL
Sbjct: 283 RMGMVHQPVEVLPLEPDGQRRQAQPAGVLPL 375
Score = 62.9 bits (131), Expect(3) = 8e-026
Identities = 28/59 (47%), Positives = 35/59 (59%)
Frame = +2 / +1
Query: 491 QAPLRLQRHLP*GPRDPREARRVLQRHRRGVQLQPDGRRAPRRERSPPCHPQCHHRRVP 667
Q +R QR LP RD +ARRVLQRH+ V++QP R A + + PQ HHRR P
Sbjct: 436 QGEVRPQRRLPRRRRDAPQARRVLQRHQGSVRVQPHHRHATQGRHAHQARPQRHHRRAP 612
Score = 33.6 bits (67), Expect(3) = 8e-026
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = +2 / +1
Query: 53 VQHRDQGVAQLPHPGPRHEARRAGGLTY 136
+Q R Q Q PHPGP +A GGL Y
Sbjct: 1 LQRRHQDHPQRPHPGPCRQAGGDGGLPY 84
>gb|BU996673.1|BU996673 HM14F23r HM Hordeum vulgare subsp. vulgare cDNA clone HM14F23
5-PRIME, mRNA sequence
Length = 628
Score = 165 bits (355), Expect(2) = 4e-082
Identities = 63/82 (76%), Positives = 69/82 (84%)
Frame = +3 / +2
Query: 297 SGAPPAPNMTEPPAGWAWSINQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSR 476
S +PP+P + E PAGWAWSINQ RSFRWNLTASAARPNPQGSYHYGQINITRTIK+ SR
Sbjct: 383 SNSPPSPKLPEAPAGWAWSINQWRSFRWNLTASAARPNPQGSYHYGQINITRTIKLSTSR 562
Query: 477 GHIDGKLRYGFNGISHRDPETP 542
G +DGK RY NG+SH D ETP
Sbjct: 563 GKVDGKERYALNGVSHADAETP 628
Score = 160 bits (345), Expect(2) = 4e-082
Identities = 63/100 (63%), Positives = 77/100 (77%)
Frame = +3 / +2
Query: 9 PMLTFEAGKTYRLRVCNTGIKASLNFRIQGHDMKLVELEGSHTLQDVYDSLDVHVGHCLS 188
PM E GKTYR RVCN GIK +LN RIQGH +KLVE+EGSHT+Q+ YDSLDVH+G CLS
Sbjct: 98 PMYNVETGKTYRFRVCNVGIKTTLNVRIQGHVVKLVEMEGSHTVQNEYDSLDVHIGQCLS 277
Query: 189 VLVDADQAPGDYYMVASTRFIHDAKSASAVIRYAGSSGAP 308
L A+Q PGDY+++ASTRFI + +AV+RY GS+ P
Sbjct: 278 FLTTANQKPGDYFLIASTRFIKGVNTITAVMRYKGSNSPP 397
Score = 127 bits (273), Expect(2) = 7e-061
Identities = 58/100 (58%), Positives = 63/100 (63%)
Frame = -2 / -2
Query: 307 GAPLEPA*RMTAEADLASWMNRVEATM**SPGAWSASTSTERQWPTCTSSESYTSCRVCE 128
G LEP *RMTA L M RVEA *SPG W A ER WP TSSESY+ C V E
Sbjct: 396 GGELEPL*RMTAVMVLTPLMKRVEAMRK*SPGFWLAVVRKERHWPMWTSSESYSFCTVWE 217
Query: 127 PSSSTSFMSWPWMRKLSDALIPVLHTRRRYVLPASKVSMG 8
PS STS +WPWMR L L+P L TR+RYVLP S + MG
Sbjct: 216 PSISTSLTTWPWMRTLRVVLMPTLQTRKRYVLPVSTLYMG 97
Score = 127 bits (272), Expect(2) = 7e-061
Identities = 55/79 (69%), Positives = 58/79 (73%)
Frame = -2 / -2
Query: 541 GVSGSLWEMPLKP*RSLPSMWPRETMTLMVRVMLIWP*W*EPCGLGRAALAVRFQRNDLA 362
GVS S WE PL+ SLPS PR +LMVRVMLIWP*W*+PCGLG AALAVRFQR DL
Sbjct: 627 GVSASAWETPLRAYLSLPSTLPRLVESLMVRVMLIWP*W*DPCGLGLAALAVRFQRKDLH 448
Query: 361 WLMDQAQPAGGSVMFGAGG 305
WLMD A PAG S G GG
Sbjct: 447 WLMDHAHPAGASGSLGDGG 391
>gb|CV055163.1|CV055163 BNEL117e7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL117e7 5' similar to Oryza sativa,
mRNA sequence
Length = 674
Score = 160 bits (345), Expect(2) = 9e-054
Identities = 67/131 (51%), Positives = 91/131 (69%)
Frame = +3 / +2
Query: 174 GHCLSVLVDADQAPGDYYMVASTRFIHDAKSASAVIRYAGSSGAPPAPNMTEPPAGWAWS 353
G LSVL AD+ Y++ S+RF + +++AV+RYAGS+G+ P + +S
Sbjct: 5 GQSLSVLFTADRPARGYHIAVSSRFTNRTLNSTAVLRYAGSTGSVSTPMRAVNRSDVDFS 184
Query: 354 INQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSRGHIDGKLRYGFNGISHRDP 533
+NQARS R NLTAS RPNPQGSYHYG IN++RTI++ S GH+ GK RYG NG+S D
Sbjct: 185 LNQARSIRTNLTASGPRPNPQGSYHYGSINVSRTIRLANSAGHVGGKPRYGVNGVSFVDA 364
Query: 534 ETPVKLAEYFN 566
+TP+KLA+Y+N
Sbjct: 365 DTPLKLADYYN 397
Score = 106 bits (226), Expect = 3e-022
Identities = 57/128 (44%), Positives = 66/128 (51%)
Frame = -2 / -3
Query: 556 SASFTGVSGSLWEMPLKP*RSLPSMWPRETMTLMVRVMLIWP*W*EPCGLGRAALAVRFQ 377
SAS GVS S E P P LP P E +LMVR+ LI P*W EPCG GR LAVRF
Sbjct: 387 SASLRGVSASTKETPFTPYLGLPPTCPAELASLMVRLTLIEP*WYEPCGFGRGPLAVRFV 208
Query: 376 RNDLAWLMDQAQPAGGSVMFGAGGAPLEPA*RMTAEADLASWMNRVEATM**SPGAWSAS 197
+ AW +++ + G P+EPA*R TA +NR E M * SA
Sbjct: 207 LMERAWFNEKSTSLRFTARIGVETEPVEPA*RSTAVELRVRLVNRDETAMW*PLAGRSAV 28
Query: 196 TSTERQWP 173
STE WP
Sbjct: 27 KSTESDWP 4
Score = 70.7 bits (148), Expect(2) = 9e-054
Identities = 25/55 (45%), Positives = 39/55 (70%)
Frame = +3 / +2
Query: 648 VITAEFRTFIEIVFENPEKSIDSLHVDGYAFFGVGMGPGTWSPEMRKTYNLLDTV 812
V+ ++ R+F+E+VFEN E + S H+DGY+ F V M G W+ E +K+YNL+D +
Sbjct: 509 VMDSDHRSFVEVVFENSEDGVQSWHLDGYSVFVVRMDKGXWNEESKKSYNLVDAM 673
>gb|BM442520.2|BM442520 EBan01_SQ003_G14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G14 5', mRNA sequence
Length = 341
Score = 204 bits (441), Expect = 7e-052
Identities = 79/106 (74%), Positives = 87/106 (82%)
Frame = +3 / +2
Query: 297 SGAPPAPNMTEPPAGWAWSINQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSR 476
S +PP+P + E PAGWAWSINQ RSFRWNLTASAARPNPQGSYHYGQINITRTIK+ SR
Sbjct: 23 SNSPPSPKLPEAPAGWAWSINQWRSFRWNLTASAARPNPQGSYHYGQINITRTIKLSTSR 202
Query: 477 GHIDGKLRYGFNGISHRDPETPVKLAEYFNATDGVFSYNQMGDVPP 614
G +DGK RY NG+ H D ETP+KLAEYFNAT GVF YN + D PP
Sbjct: 203 GKVDGKERYALNGVFHADAETPLKLAEYFNATKGVFEYNLITDTPP 340
Score = 153 bits (329), Expect = 2e-036
Identities = 69/103 (66%), Positives = 74/103 (71%)
Frame = -2 / -3
Query: 613 GGTSPIWL*LNTPSVALKYSASFTGVSGSLWEMPLKP*RSLPSMWPRETMTLMVRVMLIW 434
GG S + L NTP VALKYSAS GVS S W+ PL+ SLPS PR +LMVRVMLIW
Sbjct: 339 GGVSVMRLYSNTPLVALKYSASLRGVSASAWKTPLRAYLSLPSTLPRLVESLMVRVMLIW 160
Query: 433 P*W*EPCGLGRAALAVRFQRNDLAWLMDQAQPAGGSVMFGAGG 305
P*W*+PCGLG AALAVRFQR DL WLMD A PAG S G GG
Sbjct: 159 P*W*DPCGLGLAALAVRFQRKDLHWLMDHAHPAGASGSLGDGG 31
>gb|BE420880.1|BE420880 HWM003.E05 ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM003.E05, mRNA sequence
Length = 890
Score = 114 bits (244), Expect(3) = 5e-045
Identities = 45/112 (40%), Positives = 70/112 (62%)
Frame = +3 / +2
Query: 621 NGPLHVIPNVITAEFRTFIEIVFENPEKSIDSLHVDGYAFFGVGMGPGTWSPEMRKTYNL 800
+GP + +VI + ++ F+EIVF+N + + + H+DGYAF+ VGM G W+ R TYN
Sbjct: 344 DGPPVIGSSVINSTYKNFMEIVFQNNDTKVQTYHIDGYAFWVVGMDYGEWTDSSRGTYNK 523
Query: 801 LDTVSRHTIQVYPRSWTAIMLTFDNAGMWSVRSNVWERYYLGEQFYISVISP 956
D VSR T QV+P +WT +L D+ W+VR+ +YL ++ Y+ V+ P
Sbjct: 524 WDGVSRCTTQVFPGAWTGGLLPLDSPAFWNVRTENLGPWYLWQETYLRVVEP 679
Score = 72.1 bits (151), Expect(3) = 5e-045
Identities = 28/52 (53%), Positives = 37/52 (71%)
Frame = +3 / +2
Query: 309 PAPNMTEPPAGWAWSINQARSFRWNLTASAARPNPQGSYHYGQINITRTIKV 464
P P+ ++S+NQARS R N+T AARPNPQGS+HYGQIN+T+ K+
Sbjct: 41 PLPDPPNDEYDKSFSMNQARSIRMNVTTGAARPNPQGSFHYGQINVTQVYKL 196
Score = 34.1 bits (68), Expect(3) = 5e-045
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +3 / +2
Query: 483 IDGKLRYGFNGISHRDPETPVKLAEYF 563
I+GK R NGIS+ P TP++LA+ +
Sbjct: 218 INGKKRTTLNGISYSPPATPLRLADLY 298
>gb|CV063350.1|CV063350 BNEL89d11 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL89d11 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 593
Score = 160 bits (345), Expect(2) = 3e-044
Identities = 67/131 (51%), Positives = 91/131 (69%)
Frame = +3 / +2
Query: 174 GHCLSVLVDADQAPGDYYMVASTRFIHDAKSASAVIRYAGSSGAPPAPNMTEPPAGWAWS 353
G LSVL AD+ Y++ S+RF + +++AV+RYAGS+G+ P + +S
Sbjct: 5 GQSLSVLFTADRPARGYHIAVSSRFTNRTLNSTAVLRYAGSTGSVSTPMRAVNRSDVDFS 184
Query: 354 INQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSRGHIDGKLRYGFNGISHRDP 533
+NQARS R NLTAS RPNPQGSYHYG IN++RTI++ S GH+ GK RYG NG+S D
Sbjct: 185 LNQARSIRTNLTASGPRPNPQGSYHYGSINVSRTIRLANSAGHVGGKPRYGVNGVSFVDA 364
Query: 534 ETPVKLAEYFN 566
+TP+KLA+Y+N
Sbjct: 365 DTPLKLADYYN 397
Score = 106 bits (226), Expect = 3e-022
Identities = 57/128 (44%), Positives = 66/128 (51%)
Frame = -2 / -3
Query: 556 SASFTGVSGSLWEMPLKP*RSLPSMWPRETMTLMVRVMLIWP*W*EPCGLGRAALAVRFQ 377
SAS GVS S E P P LP P E +LMVR+ LI P*W EPCG GR LAVRF
Sbjct: 387 SASLRGVSASTKETPFTPYLGLPPTCPAELASLMVRLTLIEP*WYEPCGFGRGPLAVRFV 208
Query: 376 RNDLAWLMDQAQPAGGSVMFGAGGAPLEPA*RMTAEADLASWMNRVEATM**SPGAWSAS 197
+ AW +++ + G P+EPA*R TA +NR E M * SA
Sbjct: 207 LMERAWFNEKSTSLRFTARIGVETEPVEPA*RSTAVELRVRLVNRDETAMW*PLAGRSAV 28
Query: 196 TSTERQWP 173
STE WP
Sbjct: 27 KSTESDWP 4
Score = 39.1 bits (79), Expect(2) = 3e-044
Identities = 13/28 (46%), Positives = 21/28 (75%)
Frame = +3 / +2
Query: 648 VITAEFRTFIEIVFENPEKSIDSLHVDG 731
V+ ++ R+F+E+VFEN E + S H+DG
Sbjct: 509 VMDSDHRSFVEVVFENSEDGVQSWHLDG 592
>gb|CK125352.1|CK125352 BES1824107m07 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010M077 5-PRIME, mRNA sequence
Length = 930
Score = 133 bits (284), Expect(3) = 2e-043
Identities = 48/122 (39%), Positives = 76/122 (62%)
Frame = +3 / +2
Query: 645 NVITAEFRTFIEIVFENPEKSIDSLHVDGYAFFGVGMGPGTWSPEMRKTYNLLDTVSRHT 824
+V+ A ++ F+E+VF+N E + + H+DGY+FF VGM G W+P R +YN D +SR T
Sbjct: 455 SVLNASYKGFLEMVFQNNETDVQTYHLDGYSFFVVGMDYGEWTPNSRGSYNKWDAISRST 634
Query: 825 IQVYPRSWTAIMLTFDNAGMWSVRSNVWERYYLGEQFYISVISPARSLRDEYNMPDNALR 1004
QV+P WTA++++ DN G+W++R+ + +Y G++ Y+ V P E P L
Sbjct: 635 TQVFPGGWTAVLVSLDNVGIWNIRAEKLDNWYNGQEVYVKVADPLGYNVTEMIAPKXTLY 814
Query: 1005 CG 1010
CG
Sbjct: 815 CG 820
Score = 63.8 bits (133), Expect(3) = 2e-043
Identities = 26/53 (49%), Positives = 36/53 (67%)
Frame = +3 / +2
Query: 297 SGAPPAPNMTEPPAGWAWSINQARSFRWNLTASAARPNPQGSYHYGQINITRT 455
S + P P + +S+NQAR+ R N+++ AARPNPQGS+ YG INIT+T
Sbjct: 116 SASGPLPGAPDDFYDKYYSMNQARTIRMNVSSGAARPNPQGSFRYGSINITQT 274
Score = 24.4 bits (47), Expect(3) = 2e-043
Identities = 9/14 (64%), Positives = 11/14 (78%)
Frame = +3 / +2
Query: 210 APGDYYMVASTRFI 251
A DYY+VAS RF+
Sbjct: 8 ASTDYYIVASPRFV 49
>gb|CV058428.1|CV058428 BNEL37h7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL37h7 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 568
Score = 160 bits (345), Expect(2) = 7e-041
Identities = 67/131 (51%), Positives = 91/131 (69%)
Frame = +3 / +2
Query: 174 GHCLSVLVDADQAPGDYYMVASTRFIHDAKSASAVIRYAGSSGAPPAPNMTEPPAGWAWS 353
G LSVL AD+ Y++ S+RF + +++AV+RYAGS+G+ P + +S
Sbjct: 5 GQSLSVLFTADRPARGYHIAVSSRFTNRTLNSTAVLRYAGSTGSVSTPMRAVNRSDVDFS 184
Query: 354 INQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSRGHIDGKLRYGFNGISHRDP 533
+NQARS R NLTAS RPNPQGSYHYG IN++RTI++ S GH+ GK RYG NG+S D
Sbjct: 185 LNQARSIRTNLTASGPRPNPQGSYHYGSINVSRTIRLANSAGHVGGKPRYGVNGVSFVDA 364
Query: 534 ETPVKLAEYFN 566
+TP+KLA+Y+N
Sbjct: 365 DTPLKLADYYN 397
Score = 106 bits (226), Expect = 3e-022
Identities = 57/128 (44%), Positives = 66/128 (51%)
Frame = -2 / -2
Query: 556 SASFTGVSGSLWEMPLKP*RSLPSMWPRETMTLMVRVMLIWP*W*EPCGLGRAALAVRFQ 377
SAS GVS S E P P LP P E +LMVR+ LI P*W EPCG GR LAVRF
Sbjct: 387 SASLRGVSASTKETPFTPYLGLPPTCPAELASLMVRLTLIEP*WYEPCGFGRGPLAVRFV 208
Query: 376 RNDLAWLMDQAQPAGGSVMFGAGGAPLEPA*RMTAEADLASWMNRVEATM**SPGAWSAS 197
+ AW +++ + G P+EPA*R TA +NR E M * SA
Sbjct: 207 LMERAWFNEKSTSLRFTARIGVETEPVEPA*RSTAVELRVRLVNRDETAMW*PLAGRSAV 28
Query: 196 TSTERQWP 173
STE WP
Sbjct: 27 KSTESDWP 4
Score = 27.6 bits (54), Expect(2) = 7e-041
Identities = 9/18 (50%), Positives = 15/18 (83%)
Frame = +3 / +2
Query: 648 VITAEFRTFIEIVFENPE 701
V+ ++ R+F+E+VFEN E
Sbjct: 509 VMDSDHRSFVEVVFENSE 562
>gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07D06V 5', mRNA sequence
Length = 694
Score = 138 bits (295), Expect(2) = 1e-038
Identities = 50/100 (50%), Positives = 70/100 (70%)
Frame = +3 / +3
Query: 624 GPLHVIPNVITAEFRTFIEIVFENPEKSIDSLHVDGYAFFGVGMGPGTWSPEMRKTYNLL 803
GP ++ +V+ + R ++E+VFEN E + S H+DGYAF+ VGM G W+P R+ YNL
Sbjct: 36 GPAYLQTSVMASNMRDYVEVVFENAEGYVQSWHIDGYAFWVVGMDGGQWTPASRQNYNLR 215
Query: 804 DTVSRHTIQVYPRSWTAIMLTFDNAGMWSVRSNVWERYYL 923
D ++R+T+QVYP +WTAI + DN GMW+VRS W R YL
Sbjct: 216 DAIARYTLQVYPGAWTAIYMPLDNVGMWNVRSESWGRQYL 335
Score = 43.2 bits (88), Expect(2) = 1e-038
Identities = 19/41 (46%), Positives = 24/41 (58%)
Frame = +3 / +2
Query: 927 EQFYISVISPARSLRDEYNMPDNALRCGKVVGLPLPPSYAP 1049
+QFY+ V SPA S R E +P NAL CG+ G P +P
Sbjct: 338 QQFYLRVYSPANSWRXENPIPKNALLCGRASGRRTRPF*SP 460
>gb|CV059576.1|CV059576 BNEL49f3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL49f3 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 502
Score = 157 bits (338), Expect = 1e-037
Identities = 66/131 (50%), Positives = 90/131 (68%)
Frame = +3 / +2
Query: 174 GHCLSVLVDADQAPGDYYMVASTRFIHDAKSASAVIRYAGSSGAPPAPNMTEPPAGWAWS 353
G LSVL AD+ Y++ S+RF + +++AV+RYAGS+G+ P + +S
Sbjct: 5 GQSLSVLFTADRPARGYHIAVSSRFTNRTLNSTAVLRYAGSTGSVSTPMRAVNRSDVDFS 184
Query: 354 INQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSRGHIDGKLRYGFNGISHRDP 533
+NQARS R NLTAS RPNPQGSYHYG IN++RTI++ S H+ GK RYG NG+S D
Sbjct: 185 LNQARSIRTNLTASGPRPNPQGSYHYGSINVSRTIRLANSAXHVGGKPRYGVNGVSFVDA 364
Query: 534 ETPVKLAEYFN 566
+TP+KLA+Y+N
Sbjct: 365 DTPLKLADYYN 397
Score = 102 bits (217), Expect = 6e-021
Identities = 56/128 (43%), Positives = 65/128 (50%)
Frame = -2 / -2
Query: 556 SASFTGVSGSLWEMPLKP*RSLPSMWPRETMTLMVRVMLIWP*W*EPCGLGRAALAVRFQ 377
SAS GVS S E P P LP E +LMVR+ LI P*W EPCG GR LAVRF
Sbjct: 387 SASLRGVSASTKETPFTPYLGLPPTCXAELASLMVRLTLIEP*WYEPCGFGRGPLAVRFV 208
Query: 376 RNDLAWLMDQAQPAGGSVMFGAGGAPLEPA*RMTAEADLASWMNRVEATM**SPGAWSAS 197
+ AW +++ + G P+EPA*R TA +NR E M * SA
Sbjct: 207 LMERAWFNEKSTSLRFTARIGVETEPVEPA*RSTAVELRVRLVNRDETAMW*PLAGRSAV 28
Query: 196 TSTERQWP 173
STE WP
Sbjct: 27 KSTESDWP 4
>gb|BF253711.2|BF253711 HVSMEf0001O16f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001O16f, mRNA sequence
Length = 614
Score = 108 bits (230), Expect(2) = 1e-035
Identities = 44/86 (51%), Positives = 58/86 (67%)
Frame = +3 / +3
Query: 57 NTGIKASLNFRIQGHDMKLVELEGSHTLQDVYDSLDVHVGHCLSVLVDADQAPGDYYMVA 236
N GI S+N RIQGH + LVE+EGSHT+++ Y SLDVH+G S LV DQ P DY +V
Sbjct: 3 NVGISTSVNIRIQGHSLLLVEVEGSHTMRNTYSSLDVHLGQSYSFLVTVDQPPQDYTIVV 182
Query: 237 STRFIHDAKSASAVIRYAGSSGAPPA 314
TRF + S +A++ Y+ S+G P A
Sbjct: 183 LTRFTNPVLSNTAMLHYSNSNGVPAA 260
Score = 62.9 bits (131), Expect(2) = 1e-035
Identities = 27/53 (50%), Positives = 35/53 (66%)
Frame = +3 / +3
Query: 348 WSINQARSFRWNLTASAARPNPQGSYHYGQINITRTIKVMVSRGHIDGKLRYG 506
WS+NQARS R NL S + NP G HYG++N TRT+++ R I+ KLR G
Sbjct: 297 WSLNQARSIRGNLAVSGTQANP*GLDHYGRVNPTRTMRLANFRVTINSKLRGG 455
>gb|AV836267.1|AV836267 AV836267 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd2p19, mRNA
sequence
Length = 654
Score = 77.1 bits (162), Expect(3) = 6e-034
Identities = 31/67 (46%), Positives = 43/67 (64%)
Frame = +3 / +2
Query: 42 RLRVCNTGIKASLNFRIQGHDMKLVELEGSHTLQDVYDSLDVHVGHCLSVLVDADQAPGD 221
R RV N G+ SLN RIQGH+M +VE EGS+T++ + +LD+HVG S LV DQ
Sbjct: 2 RFRVHNVGVSTSLNLRIQGHNMAMVETEGSYTMKQNFTNLDIHVGQSYSFLVTMDQNASS 181
Query: 222 YYMVAST 242
Y + ++
Sbjct: 182 DYYIVAS 202
Score = 72.1 bits (151), Expect(3) = 6e-034
Identities = 28/52 (53%), Positives = 37/52 (71%)
Frame = +3 / +2
Query: 309 PAPNMTEPPAGWAWSINQARSFRWNLTASAARPNPQGSYHYGQINITRTIKV 464
P P+ ++S+NQARS R N+T AARPNPQGS+HYGQIN+T+ K+
Sbjct: 290 PLPDPPNDEYDKSFSMNQARSIRMNVTTGAARPNPQGSFHYGQINVTQVYKL 445
Score = 34.1 bits (68), Expect(3) = 6e-034
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +3 / +2
Query: 483 IDGKLRYGFNGISHRDPETPVKLAEYF 563
I+GK R NGIS+ P TP++LA+ +
Sbjct: 467 INGKKRTTLNGISYSPPATPLRLADLY 547
>gb|CV062756.1|CV062756 BNEL82f4 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL82f4 5' similar to
OSJNBa0084K11.18, mRNA sequence
Length = 581
Score = 144 bits (308), Expect = 2e-033
Identities = 61/130 (46%), Positives = 89/130 (68%)
Frame = +3 / +3
Query: 18 TFEAGKTYRLRVCNTGIKASLNFRIQGHDMKLVELEGSHTLQDVYDSLDVHVGHCLSVLV 197
T E GKTYRLR+ N G++++LNF IQGH++ LVE+EG+HT+Q+ Y SLDVH G LSVL
Sbjct: 18 TVEQGKTYRLRISNVGLRSTLNFMIQGHNVTLVEVEGTHTVQNAYTSLDVHAGQSLSVLF 197
Query: 198 DADQAPGDYYMVASTRFIHDAKSASAVIRYAGSSGAPPAPNMTEPPAGWAWSINQARSFR 377
AD+ Y++ S+RF + +++AV+RYAGS+G+ P + +S+NQARS R
Sbjct: 198 TADRPARGYHIAVSSRFTNRTLNSTAVLRYAGSTGSVSTPMRAVNRSDVDFSLNQARSIR 377
Query: 378 WNLTASAARP 407
+ + + RP
Sbjct: 378 YYGSRRSDRP 407
Score = 87.7 bits (185), Expect(2) = 2e-026
Identities = 47/118 (39%), Positives = 56/118 (47%)
Frame = -2 / -2
Query: 364 AWLMDQAQPAGGSVMFGAGGAPLEPA*RMTAEADLASWMNRVEATM**SPGAWSASTSTE 185
AW +++ + G P+EPA*R TA +NR E M * SA STE
Sbjct: 364 AWFNEKSTSLRFTARIGVETEPVEPA*RSTAVELRVRLVNRDETAMW*PLAGRSAVKSTE 185
Query: 184 RQWPTCTSSESYTSCRVCEPSSSTSFMSWPWMRKLSDALIPVLHTRRRYVLPASKVSM 11
WP TS E C V PS+STS WPW+ KLS P L R YV P S V +
Sbjct: 184 SDWPAWTSREV*AFCTVWVPSTSTSVTLWPWIMKLSVLRRPTLEMRSLYVFPCSTVKL 11
Score = 52.4 bits (108), Expect(2) = 2e-026
Identities = 22/33 (66%), Positives = 24/33 (72%)
Frame = -2 / -1
Query: 478 PRETMTLMVRVMLIWP*W*EPCGLGRAALAVRF 380
P E +LMVR+ LI P*W EPCG GR LAVRF
Sbjct: 581 PAELASLMVRLTLIEP*WYEPCGFGRGPLAVRF 483
>gb|AL507518.1|AL507518 AL507518 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY06B03V 5',
mRNA sequence
Length = 700
Score = 72.1 bits (151), Expect(3) = 2e-032
Identities = 28/52 (53%), Positives = 37/52 (71%)
Frame = +3 / +3
Query: 309 PAPNMTEPPAGWAWSINQARSFRWNLTASAARPNPQGSYHYGQINITRTIKV 464
P P+ ++S+NQARS R N+T AARPNPQGS+HYGQIN+T+ K+
Sbjct: 276 PLPDPPNDEYDKSFSMNQARSIRMNVTTGAARPNPQGSFHYGQINVTQVYKL 431
Score = 72.1 bits (151), Expect(3) = 2e-032
Identities = 28/62 (45%), Positives = 40/62 (64%)
Frame = +3 / +3
Query: 57 NTGIKASLNFRIQGHDMKLVELEGSHTLQDVYDSLDVHVGHCLSVLVDADQAPGDYYMVA 236
N G+ SLN RIQGH+M +VE EGS+T++ + +LD+HVG S LV DQ Y +
Sbjct: 3 NVGVSTSLNLRIQGHNMAMVETEGSYTMKQNFTNLDIHVGQSYSFLVTMDQNASSDYYIV 182
Query: 237 ST 242
++
Sbjct: 183 AS 188
Score = 34.1 bits (68), Expect(3) = 2e-032
Identities = 13/27 (48%), Positives = 19/27 (70%)
Frame = +3 / +3
Query: 483 IDGKLRYGFNGISHRDPETPVKLAEYF 563
I+GK R NGIS+ P TP++LA+ +
Sbjct: 453 INGKKRTTLNGISYSPPATPLRLADLY 533
>gb|AJ461534.1|AJ461534 AJ461534 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200012C08F1, mRNA sequence
Length = 300
Score = 126 bits (269), Expect = 4e-028
Identities = 44/89 (49%), Positives = 65/89 (73%)
Frame = +3 / +1
Query: 762 GTWSPEMRKTYNLLDTVSRHTIQVYPRSWTAIMLTFDNAGMWSVRSNVWERYYLGEQFYI 941
G W+ R YNL+D +RHT+QVYP W+AI+++ DN GMW++RS W+R+YLG+Q Y+
Sbjct: 13 GQWTENERLNYNLVDAQARHTVQVYPNGWSAILVSLDNQGMWNLRSANWDRHYLGQQLYL 192
Query: 942 SVISPARSLRDEYNMPDNALRCGKVVGLP 1028
V + +SL +EY++P NA+ CG+ GLP
Sbjct: 193 RVWTAEQSLSNEYSIPTNAILCGRADGLP 279
>gb|CB880252.1|CB880252 HM05P11w HM Hordeum vulgare subsp. vulgare cDNA clone HM05P11
3-PRIME, mRNA sequence
Length = 664
Score = 122 bits (261), Expect = 5e-027
Identities = 49/97 (50%), Positives = 68/97 (70%)
Frame = +3 / +1
Query: 9 PMLTFEAGKTYRLRVCNTGIKASLNFRIQGHDMKLVELEGSHTLQDVYDSLDVHVGHCLS 188
P T+ + + +++LN RIQGH +KLVE+EGSHT+Q+ YDSLDVH+G CLS
Sbjct: 355 PCTTWRPARRTASASATSASRSTLNVRIQGHVVKLVEMEGSHTVQNEYDSLDVHIGQCLS 534
Query: 189 VLVDADQAPGDYYMVASTRFIHDAKSASAVIRYAGSS 299
L A+Q PGDY+++ASTRFI + +AV+RY GS+
Sbjct: 535 FLTTANQKPGDYFLIASTRFIKGVHTITAVMRYKGSN 645
Score = 94.1 bits (199), Expect(2) = 6e-022
Identities = 43/73 (58%), Positives = 45/73 (61%)
Frame = -2 / -3
Query: 298 LEPA*RMTAEADLASWMNRVEATM**SPGAWSASTSTERQWPTCTSSESYTSCRVCEPSS 119
LEP *RMTA M RVEA *SPG W A ER WP TSSESY+ C V EPS
Sbjct: 644 LEPL*RMTAVMVXTPLMKRVEAMRK*SPGFWLAVVRKERHWPMWTSSESYSFCTVWEPSI 465
Query: 118 STSFMSWPWMRKL 80
STS +WPWMR L
Sbjct: 464 STSLTTWPWMRTL 426
Score = 30.9 bits (61), Expect(2) = 6e-022
Identities = 15/22 (68%), Positives = 15/22 (68%)
Frame = -1 / -3
Query: 71 LDPGVAHAEAVRLARLEGEHGR 6
LD VA AEAVRLA L HGR
Sbjct: 416 LDADVADAEAVRLAGLHVVHGR 351
>gb|AJ462026.1|AJ462026 AJ462026 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200079D08F1, mRNA sequence
Length = 300
Score = 110 bits (235), Expect = 2e-023
Identities = 43/80 (53%), Positives = 55/80 (68%)
Frame = +3 / +1
Query: 810 VSRHTIQVYPRSWTAIMLTFDNAGMWSVRSNVWERYYLGEQFYISVISPARSLRDEYNMP 989
++R+T+QVYP +WTAI + DN GMW+VRS W R YLG+QFY+ V SPA S RDE +P
Sbjct: 1 IARYTLQVYPGAWTAIYMPLDNVGMWNVRSESWGRQYLGQQFYLRVYSPANSWRDENPIP 180
Query: 990 DNALRCGKVVGLPLPPSYAP 1049
NAL CG+ G P +P
Sbjct: 181 KNALLCGRASGRRTRPL*SP 240
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 626,934,198
Number of Sequences: 312970
Number of extensions: 10852075
Number of successful extensions: 138421
Number of sequences better than 1.0e-020: 25
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 18220775284
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)