TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.3
(1548 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow st... 161 1e-061
gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow st... 222 3e-057
gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow st... 116 4e-048
gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow st... 168 6e-041
gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. v... 157 1e-040
gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow st... 96 2e-040
gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow st... 96 2e-039
gb|BQ464404.1|BQ464404 HF02C24r HF Hordeum vulgare subsp. v... 138 5e-035
gb|BQ470839.1|BQ470839 HX04D02r HX Hordeum vulgare subsp. v... 114 1e-030
gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow st... 96 4e-027
gb|BG301028.1|BG301028 HVSMEb0019E22f Hordeum vulgare seedl... 74 2e-025
gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachi... 109 4e-023
gb|BG365247.2|BG365247 HVSMEi0002A02f Hordeum vulgare 20 DA... 59 7e-023
gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow st... 81 2e-022
gb|CA030305.1|CA030305 HX06K19r HX Hordeum vulgare subsp. v... 68 4e-021
gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke devel... 74 6e-021
>gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G09 5', mRNA sequence
Length = 588
Score = 161 bits (347), Expect(2) = 1e-061
Identities = 64/90 (71%), Positives = 69/90 (76%)
Frame = -3 / +3
Query: 751 GVKCGPGHGISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLY 572
GV CGPGHGIS+GSLGRYKDEKDV D+ V C G+TNGLRIKSYEDSKS L ASK Y
Sbjct: 3 GVTCGPGHGISIGSLGRYKDEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISY 182
Query: 571 EGITMDNVSYPIIIDQKYCPNNICVKSGAS 482
E I MD+ YPIIIDQKYCPN +C G S
Sbjct: 183 ENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 272
Score = 96.4 bits (204), Expect(2) = 1e-061
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = -3 / +3
Query: 496 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 317
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 261 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 440
Query: 316 KNAIGKSIGLAKELAC 269
N + G+ K AC
Sbjct: 441 TNVKVTATGVDKANAC 488
Score = 64.8 bits (135), Expect(3) = 4e-022
Identities = 34/55 (61%), Positives = 37/55 (67%)
Frame = +3 / -3
Query: 483 EAPDLTQMLLGQYFWSMMMG*ETLSMVMPSYRNLLALSDDLESSYDLMRRPFVVP 647
E+P Q LLGQYF SMMMG* S++M S L A DLESSYDLMRRP V P
Sbjct: 271 ESPLAVQSLLGQYF*SMMMG*PESSILMFS*LILDATRGDLESSYDLMRRPLVDP 107
Score = 49.6 bits (102), Expect(3) = 4e-022
Identities = 25/46 (54%), Positives = 28/46 (60%)
Frame = +2 / -2
Query: 320 DGHGLVAGSIVLDVDVDELHALAWQVVGAVERDGLRRVGGAVDVLE 457
D HGLV + VLD+DV A A VGA + DGLR GGA DV E
Sbjct: 437 DSHGLVVLAGVLDLDVGHCDAFARLFVGAEQADGLRGGGGAGDVFE 300
Score = 28.6 bits (56), Expect(3) = 4e-022
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +3 / -3
Query: 723 MPWPGPHLTP 752
MPWPGP +TP
Sbjct: 31 MPWPGPQVTP 2
>gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L02 5', mRNA sequence
Length = 377
Score = 222 bits (480), Expect = 3e-057
Identities = 89/124 (71%), Positives = 97/124 (78%)
Frame = -3 / +2
Query: 859 GIHIGDSSNVTISSTTIGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDV 680
GIH+GDSSNV I TTI VGDDCISIGPGSK + I GV CGP HGIS+GSLGRYKDEKDV
Sbjct: 2 GIHMGDSSNVNIIDTTIAVGDDCISIGPGSKQVNISGVTCGPSHGISIGSLGRYKDEKDV 181
Query: 679 EDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNIC 500
D+ V C G+TNGLRIKSYEDSKS L ASK YE I MD+ SYPIIIDQKYCP+ +C
Sbjct: 182 TDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSSYPIIIDQKYCPHKLC 361
Query: 499 VKSG 488
G
Sbjct: 362 TAKG 373
>gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_F02 5', mRNA sequence
Length = 693
Score = 116 bits (248), Expect(2) = 4e-048
Identities = 47/71 (66%), Positives = 51/71 (71%)
Frame = -3 / +2
Query: 694 DEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYC 515
DEKDV D+ V C G+TNGLRIKSYEDSKS L ASK YE I MD+ YPIIIDQKYC
Sbjct: 2 DEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYC 181
Query: 514 PNNICVKSGAS 482
PN +C G S
Sbjct: 182 PNKLCTAKGDS 214
Score = 96.4 bits (204), Expect(2) = 4e-048
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = -3 / +2
Query: 496 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 317
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 203 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 382
Query: 316 KNAIGKSIGLAKELAC 269
N + G+ K AC
Sbjct: 383 TNVKVTATGVDKANAC 430
>gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_F12 5', mRNA sequence
Length = 311
Score = 168 bits (362), Expect = 6e-041
Identities = 66/102 (64%), Positives = 79/102 (77%)
Frame = -3 / +3
Query: 1114 ENLSINGHGTIDGQGALVWSKNQCQHSYNCKILPNSLVLDFVTNVQIRGITLLNSKFFHL 935
+NL I G G+ID QG VW+KN CQ +YNCKILPNSLVLDF + I I+++NSKFFH+
Sbjct: 3 KNLVITGKGSIDRQGKAVWTKNSCQKNYNCKILPNSLVLDFCDDALIESISIINSKFFHM 182
Query: 934 NIFECKNVLIDKVTVKAPGDSPNTDGIHIGDSSNVTISSTTI 809
NI+ECK V++ V V APGDSPNTDGIH+GDS NV I TTI
Sbjct: 183 NIYECKGVIVKDVKVSAPGDSPNTDGIHMGDSFNVNIIDTTI 308
Score = 106 bits (227), Expect = 3e-022
Identities = 53/91 (58%), Positives = 58/91 (63%)
Frame = +3 / -2
Query: 840 ESPMWMPSVLGLSPGALTVTLSISTFLHSKMLRWKNLLLSSVMPRIWTFVTKSSTKLFGR 1019
ESPMWMPSVLGLSPGALT T T LHS M WKNL L M + KSSTK FG+
Sbjct: 277 ESPMWMPSVLGLSPGALTFTSLTITPLHS*MFMWKNLELMIEMLSMSASSQKSSTKEFGK 98
Query: 1020 ILQL*ECWHWFLLHTRAPCPSMVPWPLMDRF 1112
ILQL* W FL+ T P S+ P P+M RF
Sbjct: 97 ILQL*FFWQLFLVQTALP*RSIDPLPVMTRF 5
>gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. vulgare cDNA clone HM10P12
3-PRIME, mRNA sequence
Length = 608
Score = 157 bits (338), Expect(2) = 1e-040
Identities = 62/150 (41%), Positives = 95/150 (63%)
Frame = -3 / +3
Query: 1027 CKILPNSLVLDFVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHI 848
CK P ++ +D V++RG+T+ N++ HL + + V +D + ++APGDSPNTDGIH+
Sbjct: 81 CKGAPTAVTIDSCRGVRVRGLTIQNAQQMHLTVSRSRGVRLDGMAIQAPGDSPNTDGIHV 260
Query: 847 GDSSNVTISSTTIGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDVEDVQ 668
+S+ VTI+ IG GDDC+SI S +++ G+ C PGHGIS+GSLG+ VE V
Sbjct: 261 AESTAVTITGARIGTGDDCVSISNASFAVKMKGIVCDPGHGISIGSLGQGGSYAAVEGVT 440
Query: 667 VTGCTIAGTTNGLRIKSYEDSKSSLKASKF 578
+ G IA NG+RIK+ + ++ +F
Sbjct: 441 LDGARIARAQNGVRIKTXQGGAGYVRNVRF 530
Score = 30.4 bits (60), Expect(2) = 1e-040
Identities = 10/20 (50%), Positives = 14/20 (70%)
Frame = -3 / +3
Query: 574 YEGITMDNVSYPIIIDQKYC 515
+ + +D V +PIIIDQ YC
Sbjct: 528 FTNVLVDGVDHPIIIDQFYC 587
>gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E14 5', mRNA sequence
Length = 454
Score = 95.9 bits (203), Expect(2) = 2e-040
Identities = 39/55 (70%), Positives = 42/55 (76%)
Frame = -3 / +3
Query: 646 GTTNGLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGAS 482
G+TNGLRIKSYEDSKS L ASK YE I MD+ YPIIIDQKYCPN +C G S
Sbjct: 15 GSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 179
Score = 91.3 bits (193), Expect(2) = 2e-040
Identities = 38/76 (50%), Positives = 52/76 (68%)
Frame = -3 / +3
Query: 496 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 317
K +S+V V DV FKNI GTS+TPEA++L C++ P +GV + +V I+Y+G NKTMAVC
Sbjct: 168 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPYEGVTMSDVKIEYSGKNNKTMAVC 347
Query: 316 KNAIGKSIGLAKELAC 269
N + G+ K AC
Sbjct: 348 TNVKVTATGVDKANAC 395
>gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_L07 5', mRNA sequence
Length = 436
Score = 96.4 bits (204), Expect(2) = 2e-039
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = -3 / +2
Query: 496 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 317
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 143 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 322
Query: 316 KNAIGKSIGLAKELAC 269
N + G+ K AC
Sbjct: 323 TNVKVTATGVDKANAC 370
Score = 87.7 bits (185), Expect(2) = 2e-039
Identities = 36/51 (70%), Positives = 38/51 (74%)
Frame = -3 / +2
Query: 634 GLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGAS 482
GLRIKSYEDSKS L ASK YE I MD+ YPIIIDQKYCPN +C G S
Sbjct: 2 GLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 154
>gb|BQ464404.1|BQ464404 HF02C24r HF Hordeum vulgare subsp. vulgare cDNA clone HF02C24
5-PRIME, mRNA sequence
Length = 579
Score = 138 bits (297), Expect(2) = 5e-035
Identities = 59/138 (42%), Positives = 85/138 (61%)
Frame = -3 / +2
Query: 1024 KILPNSLVLDFVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHIG 845
++ P +L +NV + GIT++NS HL C+ VL+ +T+ +P +S NTDGIH+
Sbjct: 152 RVKPTALRFYGSSNVVVAGITIVNSSQCHLKFDNCQGVLVHDLTISSPENSLNTDGIHLQ 331
Query: 844 DSSNVTISSTTIGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDVEDVQV 665
+S +V+I T + GDDCISI G I IH V CGPGHGIS+G LGR + V +V V
Sbjct: 332 NSKDVSIHHTNLACGDDCISIQTGCSNIYIHNVNCGPGHGISIGGLGRDNTKACVSNVTV 511
Query: 664 TGCTIAGTTNGLRIKSYE 611
+ T G+RIK+++
Sbjct: 512 RDVNMFRTMTGVRIKTWQ 565
Score = 29.9 bits (59), Expect(2) = 5e-035
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -3 / +2
Query: 1132 IEIENVENLSINGHGTIDGQGALVWS 1055
+E + LSI G GTI+G+G W+
Sbjct: 11 LEFTKLSGLSIQGSGTINGRGQQWWT 88
>gb|BQ470839.1|BQ470839 HX04D02r HX Hordeum vulgare subsp. vulgare cDNA clone HX04D02
5-PRIME, mRNA sequence
Length = 650
Score = 114 bits (243), Expect(3) = 1e-030
Identities = 50/138 (36%), Positives = 77/138 (55%)
Frame = -3 / +2
Query: 991 VTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHIGDSSNVTISSTT 812
V V+++G+TL N + HL C N + V +P SP+TDGI + +S +V I+
Sbjct: 50 VHGVRVQGVTLQNGQQHHLTFTRCSNARASFLRVASPESSPDTDGIRLVNSISVHINDIH 229
Query: 811 IGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDVEDVQVTGCTIAGTTNG 632
I G C+S+ +R+ + CGPG+GIS+GS+G +E ++V IA TTNG
Sbjct: 230 IATGGACVSMAGNCTDVRLRYISCGPGNGISIGSIGETPVADRLEKIEVDTMFIANTTNG 409
Query: 631 LRIKSYEDSKSSLKASKF 578
L IK+++D + KF
Sbjct: 410 LLIKTWQDGCGYARKVKF 463
Score = 29.0 bits (57), Expect(3) = 1e-030
Identities = 11/27 (40%), Positives = 18/27 (66%)
Frame = -3 / +2
Query: 478 VAVNDVVFKNIHGTSNTPEAITLNCAN 398
VAV + + NI GTS + A+T +C++
Sbjct: 563 VAVEKIDYVNIAGTSASKRAVTFSCSD 643
Score = 28.6 bits (56), Expect(3) = 1e-030
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -3 / +2
Query: 589 ASKFLYEGITMDNVSYPIIIDQ 524
A K + + M NVS PIIIDQ
Sbjct: 446 ARKVKFANVVMKNVSNPIIIDQ 511
>gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_C11 5', mRNA sequence
Length = 647
Score = 96.4 bits (204), Expect(2) = 4e-027
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = -3 / +1
Query: 496 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 317
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 64 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 243
Query: 316 KNAIGKSIGLAKELAC 269
N + G+ K AC
Sbjct: 244 TNVKVTATGVDKANAC 291
Score = 46.0 bits (94), Expect(2) = 4e-027
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = -3 / +1
Query: 556 DNVSYPIIIDQKYCPNNICVKSGAS 482
D+ YPIIIDQKYCPN +C G S
Sbjct: 1 DDSGYPIIIDQKYCPNKLCTAKGDS 75
>gb|BG301028.1|BG301028 HVSMEb0019E22f Hordeum vulgare seedling shoot EST library HVcDNA0002
(Dehydration stress) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEb0019E22f, mRNA sequence
Length = 635
Score = 73.9 bits (155), Expect(3) = 2e-025
Identities = 27/81 (33%), Positives = 48/81 (59%)
Frame = -3 / +3
Query: 1027 CKILPNSLVLDFVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHI 848
CK P ++ +D V++R +T+ N++ HL + + V +D + ++APGDSPNTDGIH+
Sbjct: 393 CKGAPTAVTIDSCRGVRVRDLTIQNAQQMHLTVSRSRGVRLDGMAIQAPGDSPNTDGIHV 572
Query: 847 GDSSNVTISSTTIGVGDDCIS 785
+S+ + + C+S
Sbjct: 573 AESTR*PLPARASVPATTCVS 635
Score = 53.8 bits (111), Expect(3) = 2e-025
Identities = 21/73 (28%), Positives = 34/73 (46%)
Frame = -3 / +3
Query: 1363 IAQLGAKGDGKSDSTPMILKAWKNACEATGVQKIVIPPGNYLTGGLELKGPCKSSIIIRL 1184
+ GA GDG +D T KAW+ AC +V Y G + GPCK +++ +
Sbjct: 42 VDSFGAVGDGCADDTEAFAKAWEKACSLKDAVLVVTAGRRYKVGPSKFMGPCKERLVVLI 221
Query: 1183 DGNLLGTGDLSAY 1145
G ++ + S +
Sbjct: 222 HGTIVAPEEPSQW 260
Score = 26.7 bits (52), Expect(3) = 2e-025
Identities = 10/31 (32%), Positives = 15/31 (48%)
Frame = -3 / +3
Query: 1141 RNWIEIENVENLSINGHGTIDGQGALVWSKN 1049
R W+ + I G G IDG G+ W+ +
Sbjct: 276 RLWLLFGGLVGARIQGGGVIDGSGSKWWANS 368
>gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachis EST library HVcDNA0015 (normal)
Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0008L17f,
mRNA sequence
Length = 458
Score = 109 bits (233), Expect = 4e-023
Identities = 42/104 (40%), Positives = 65/104 (62%)
Frame = -3 / +1
Query: 1015 PNSLVLDFVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDGIHIGDSS 836
P +L NV ++G+ + NS FH C+ V + +++ +P SPNTDGIH+ ++
Sbjct: 52 PVALRFFMSNNVTVQGLKVQNSPEFHFRFDSCRGVRVYSLSIISPALSPNTDGIHVENTQ 231
Query: 835 NVTISSTTIGVGDDCISIGPGSKMIRIHGVKCGPGHGISVGSLG 704
+V I++T + GD+C+ IG G+ + I V CGPGHGI +GSLG
Sbjct: 232 DVLITNTVVSNGDNCVCIGAGTLNVHIENVTCGPGHGIIIGSLG 363
>gb|BG365247.2|BG365247 HVSMEi0002A02f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0002A02f, mRNA sequence
Length = 744
Score = 59.3 bits (123), Expect(4) = 7e-023
Identities = 22/57 (38%), Positives = 36/57 (63%)
Frame = -3 / +3
Query: 1027 CKILPNSLVLDFVTNVQIRGITLLNSKFFHLNIFECKNVLIDKVTVKAPGDSPNTDG 857
C P +L D N+Q+ + L+NS+ HL++ +C V + ++++ APG SPNTDG
Sbjct: 558 CTKAPTALTFDQCNNLQVDNLKLVNSQQMHLSVEDCSAVQLARLSLTAPGTSPNTDG 728
Score = 40.5 bits (82), Expect(4) = 7e-023
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -3 / +3
Query: 1351 GAKGDGKSDSTPMILKAWKNACEATGVQKIVIPPG 1247
GA+GDGK D + KAW AC ++ +++P G
Sbjct: 213 GARGDGKHDDAQPLAKAWNAACSSSRPAVLLVPEG 317
Score = 36.8 bits (74), Expect(4) = 7e-023
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -3 / +3
Query: 1135 WIEIENVENLSINGHGTIDGQGALVWSKN 1049
WI I+ V L+++G GTI+G G + WS +
Sbjct: 447 WILIQGVTGLTVSGGGTINGNGDVWWSNS 533
Score = 26.3 bits (51), Expect(4) = 7e-023
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -3 / +3
Query: 1243 YLTGGLELKGPCKSSIII 1190
YL + L GPCKSS ++
Sbjct: 324 YLLSPVTLSGPCKSSSVV 377
>gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_B18 5', mRNA sequence
Length = 431
Score = 81.3 bits (171), Expect(2) = 2e-022
Identities = 32/54 (59%), Positives = 39/54 (72%)
Frame = -3 / +1
Query: 1366 DIAQLGAKGDGKSDSTPMILKAWKNACEATGVQKIVIPPGNYLTGGLELKGPCK 1205
DI +LGAK DGK+DST + +AW +AC TG Q IVIP G++LTG L GPCK
Sbjct: 160 DIIKLGAKPDGKTDSTKAVEEAWASACGGTGKQTIVIPKGDFLTGALNFTGPCK 321
Score = 45.5 bits (93), Expect(2) = 2e-022
Identities = 17/33 (51%), Positives = 24/33 (72%)
Frame = -3 / +1
Query: 1198 IIIRLDGNLLGTGDLSAYQRNWIEIENVENLSI 1100
+ I+L+GNLL + DLS + NWIEI ++NL I
Sbjct: 331 VTIKLEGNLLASNDLSMFPSNWIEIMRIKNLVI 429
>gb|CA030305.1|CA030305 HX06K19r HX Hordeum vulgare subsp. vulgare cDNA clone HX06K19
5-PRIME, mRNA sequence
Length = 546
Score = 67.5 bits (141), Expect(3) = 4e-021
Identities = 28/75 (37%), Positives = 43/75 (57%)
Frame = -3 / +1
Query: 802 GDDCISIGPGSKMIRIHGVKCGPGHGISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRI 623
G C+S+ +R+ + CGPG+GIS+GS+G +E ++V IA TTNGL I
Sbjct: 22 GGACVSMAGNCTDVRLRYISCGPGNGISIGSIGETPVADRLEKIEVDTMFIANTTNGLLI 201
Query: 622 KSYEDSKSSLKASKF 578
K+++D + KF
Sbjct: 202 KTWQDGCGYARKVKF 246
Score = 43.7 bits (89), Expect(3) = 4e-021
Identities = 17/42 (40%), Positives = 29/42 (69%)
Frame = -3 / +1
Query: 478 VAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIK 353
VAV + + NI GTS + A+T +C++ +PC+ V L +V++K
Sbjct: 346 VAVEKIDYVNIAGTSASKRAVTFSCSDVVPCRQVSLKDVNLK 471
Score = 28.6 bits (56), Expect(3) = 4e-021
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -3 / +1
Query: 589 ASKFLYEGITMDNVSYPIIIDQ 524
A K + + M NVS PIIIDQ
Sbjct: 229 ARKVKFANVVMKNVSNPIIIDQ 294
>gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04C10T 5',
mRNA sequence
Length = 507
Score = 73.9 bits (155), Expect(2) = 6e-021
Identities = 28/73 (38%), Positives = 48/73 (65%)
Frame = -3 / +2
Query: 574 YEGITMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANN 395
+ + +D V +PIIIDQ YC + + ++ VAV++V+++NI GTS EAI L C++
Sbjct: 167 FTNVLVDGVDHPIIIDQFYCDSRTPCANRSTNVAVSNVMYRNISGTSTRDEAIKLACSDA 346
Query: 394 LPCQGVQLVNVDI 356
+PC + L N+++
Sbjct: 347 VPCSDIVLSNINL 385
Score = 47.8 bits (98), Expect(2) = 6e-021
Identities = 21/52 (40%), Positives = 31/52 (59%)
Frame = -3 / +2
Query: 733 GHGISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKF 578
GHGIS+GSLG+ VE V + G IA NG+RIK+++ ++ +F
Sbjct: 14 GHGISIGSLGQGGSYAAVEGVTLDGARIARAQNGVRIKTWQGGAGYVRNVRF 169
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 511,051,264
Number of Sequences: 312970
Number of extensions: 7200768
Number of successful extensions: 74578
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 19307335278
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)