TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.2
(682 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow st... 141 6e-056
gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow st... 116 2e-048
gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow st... 96 7e-041
gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow st... 96 6e-040
gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow st... 139 1e-032
gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow st... 96 2e-027
>gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G09 5', mRNA sequence
Length = 588
Score = 141 bits (302), Expect(2) = 6e-056
Identities = 57/82 (69%), Positives = 62/82 (75%)
Frame = +1 / +3
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNV 180
GIS+GSLGRYKDEKDV D+ V C G+TNGLRIKSYEDSKS L ASK YE I MD+
Sbjct: 27 GISIGSLGRYKDEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDS 206
Query: 181 SYPIIIDQKYCPNNICVKSGAS 246
YPIIIDQKYCPN +C G S
Sbjct: 207 GYPIIIDQKYCPNKLCTAKGDS 272
Score = 96.4 bits (204), Expect(2) = 6e-056
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = +1 / +3
Query: 232 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 411
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 261 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 440
Query: 412 KNAIGKSIGLAKELAC 459
N + G+ K AC
Sbjct: 441 TNVKVTATGVDKANAC 488
>gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_F02 5', mRNA sequence
Length = 693
Score = 116 bits (248), Expect(2) = 2e-048
Identities = 47/71 (66%), Positives = 51/71 (71%)
Frame = +1 / +2
Query: 34 DEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYC 213
DEKDV D+ V C G+TNGLRIKSYEDSKS L ASK YE I MD+ YPIIIDQKYC
Sbjct: 2 DEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYC 181
Query: 214 PNNICVKSGAS 246
PN +C G S
Sbjct: 182 PNKLCTAKGDS 214
Score = 96.4 bits (204), Expect(2) = 2e-048
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = +1 / +2
Query: 232 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 411
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 203 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 382
Query: 412 KNAIGKSIGLAKELAC 459
N + G+ K AC
Sbjct: 383 TNVKVTATGVDKANAC 430
>gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E14 5', mRNA sequence
Length = 454
Score = 95.9 bits (203), Expect(2) = 7e-041
Identities = 39/55 (70%), Positives = 42/55 (76%)
Frame = +1 / +3
Query: 82 GTTNGLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGAS 246
G+TNGLRIKSYEDSKS L ASK YE I MD+ YPIIIDQKYCPN +C G S
Sbjct: 15 GSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 179
Score = 91.3 bits (193), Expect(2) = 7e-041
Identities = 38/76 (50%), Positives = 52/76 (68%)
Frame = +1 / +3
Query: 232 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 411
K +S+V V DV FKNI GTS+TPEA++L C++ P +GV + +V I+Y+G NKTMAVC
Sbjct: 168 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPYEGVTMSDVKIEYSGKNNKTMAVC 347
Query: 412 KNAIGKSIGLAKELAC 459
N + G+ K AC
Sbjct: 348 TNVKVTATGVDKANAC 395
>gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_L07 5', mRNA sequence
Length = 436
Score = 96.4 bits (204), Expect(2) = 6e-040
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = +1 / +2
Query: 232 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 411
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 143 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 322
Query: 412 KNAIGKSIGLAKELAC 459
N + G+ K AC
Sbjct: 323 TNVKVTATGVDKANAC 370
Score = 87.7 bits (185), Expect(2) = 6e-040
Identities = 36/51 (70%), Positives = 38/51 (74%)
Frame = +1 / +2
Query: 94 GLRIKSYEDSKSSLKASKFLYEGITMDNVSYPIIIDQKYCPNNICVKSGAS 246
GLRIKSYEDSKS L ASK YE I MD+ YPIIIDQKYCPN +C G S
Sbjct: 2 GLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 154
>gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L02 5', mRNA sequence
Length = 377
Score = 139 bits (299), Expect = 1e-032
Identities = 56/80 (70%), Positives = 62/80 (77%)
Frame = +1 / +2
Query: 1 GISVGSLGRYKDEKDVEDVQVTGCTIAGTTNGLRIKSYEDSKSSLKASKFLYEGITMDNV 180
GIS+GSLGRYKDEKDV D+ V C G+TNGLRIKSYEDSKS L ASK YE I MD+
Sbjct: 134 GISIGSLGRYKDEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDS 313
Query: 181 SYPIIIDQKYCPNNICVKSG 240
SYPIIIDQKYCP+ +C G
Sbjct: 314 SYPIIIDQKYCPHKLCTAKG 373
>gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_C11 5', mRNA sequence
Length = 647
Score = 96.4 bits (204), Expect(2) = 2e-027
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = +1 / +1
Query: 232 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLVNVDIKYNGSGNKTMAVC 411
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+G NKTMAVC
Sbjct: 64 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 243
Query: 412 KNAIGKSIGLAKELAC 459
N + G+ K AC
Sbjct: 244 TNVKVTATGVDKANAC 291
Score = 46.0 bits (94), Expect(2) = 2e-027
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +1 / +1
Query: 172 DNVSYPIIIDQKYCPNNICVKSGAS 246
D+ YPIIIDQKYCPN +C G S
Sbjct: 1 DDSGYPIIIDQKYCPNKLCTAKGDS 75
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 275,941,485
Number of Sequences: 312970
Number of extensions: 4425008
Number of successful extensions: 68637
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7422924075
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)