TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115184.2.1
(642 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow st... 144 1e-055
gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow st... 113 3e-046
gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow st... 93 1e-038
gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow st... 92 9e-038
gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow st... 140 8e-033
gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow st... 92 4e-026
gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke devel... 75 1e-021
>gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G09 5', mRNA sequence
Length = 588
Score = 144 bits (309), Expect(2) = 1e-055
Identities = 56/84 (66%), Positives = 65/84 (77%)
Frame = +3 / +3
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKFLYQDVTMD 182
GHGIS+GSLGRYKDEKDV D+ V C G+TNGLRIKSYEDSKS L A+K Y+++ MD
Sbjct: 21 GHGISIGSLGRYKDEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMD 200
Query: 183 NVSYPIIIDQKYCPNNICVKSGAS 254
+ YPIIIDQKYCPN +C G S
Sbjct: 201 DSGYPIIIDQKYCPNKLCTAKGDS 272
Score = 91.8 bits (194), Expect(2) = 1e-055
Identities = 37/76 (48%), Positives = 53/76 (69%)
Frame = +3 / +3
Query: 240 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLINVDIKYNRSDNKTMSVC 419
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+ +NKTM+VC
Sbjct: 261 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 440
Query: 420 KNAIGKSIGMAKELAC 467
N + G+ K AC
Sbjct: 441 TNVKVTATGVDKANAC 488
>gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_F02 5', mRNA sequence
Length = 693
Score = 113 bits (241), Expect(2) = 3e-046
Identities = 44/71 (61%), Positives = 52/71 (73%)
Frame = +3 / +2
Query: 42 DEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKFLYQDVTMDNVSYPIIIDQKYC 221
DEKDV D+ V C G+TNGLRIKSYEDSKS L A+K Y+++ MD+ YPIIIDQKYC
Sbjct: 2 DEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYC 181
Query: 222 PNNICVKSGAS 254
PN +C G S
Sbjct: 182 PNKLCTAKGDS 214
Score = 91.8 bits (194), Expect(2) = 3e-046
Identities = 37/76 (48%), Positives = 53/76 (69%)
Frame = +3 / +2
Query: 240 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLINVDIKYNRSDNKTMSVC 419
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+ +NKTM+VC
Sbjct: 203 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 382
Query: 420 KNAIGKSIGMAKELAC 467
N + G+ K AC
Sbjct: 383 TNVKVTATGVDKANAC 430
>gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E14 5', mRNA sequence
Length = 454
Score = 93.2 bits (197), Expect(2) = 1e-038
Identities = 36/55 (65%), Positives = 43/55 (78%)
Frame = +3 / +3
Query: 90 GTTNGLRIKSYEDSKSSLKATKFLYQDVTMDNVSYPIIIDQKYCPNNICVKSGAS 254
G+TNGLRIKSYEDSKS L A+K Y+++ MD+ YPIIIDQKYCPN +C G S
Sbjct: 15 GSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 179
Score = 86.8 bits (183), Expect(2) = 1e-038
Identities = 36/76 (47%), Positives = 52/76 (68%)
Frame = +3 / +3
Query: 240 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLINVDIKYNRSDNKTMSVC 419
K +S+V V DV FKNI GTS+TPEA++L C++ P +GV + +V I+Y+ +NKTM+VC
Sbjct: 168 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPYEGVTMSDVKIEYSGKNNKTMAVC 347
Query: 420 KNAIGKSIGMAKELAC 467
N + G+ K AC
Sbjct: 348 TNVKVTATGVDKANAC 395
>gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_L07 5', mRNA sequence
Length = 436
Score = 91.8 bits (194), Expect(2) = 9e-038
Identities = 37/76 (48%), Positives = 53/76 (69%)
Frame = +3 / +2
Query: 240 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLINVDIKYNRSDNKTMSVC 419
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+ +NKTM+VC
Sbjct: 143 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 322
Query: 420 KNAIGKSIGMAKELAC 467
N + G+ K AC
Sbjct: 323 TNVKVTATGVDKANAC 370
Score = 84.9 bits (179), Expect(2) = 9e-038
Identities = 33/51 (64%), Positives = 39/51 (76%)
Frame = +3 / +2
Query: 102 GLRIKSYEDSKSSLKATKFLYQDVTMDNVSYPIIIDQKYCPNNICVKSGAS 254
GLRIKSYEDSKS L A+K Y+++ MD+ YPIIIDQKYCPN +C G S
Sbjct: 2 GLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 154
>gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L02 5', mRNA sequence
Length = 377
Score = 140 bits (300), Expect = 8e-033
Identities = 54/81 (66%), Positives = 64/81 (79%)
Frame = +3 / +2
Query: 6 HGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKFLYQDVTMDN 185
HGIS+GSLGRYKDEKDV D+ V C G+TNGLRIKSYEDSKS L A+K Y+++ MD+
Sbjct: 131 HGISIGSLGRYKDEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDD 310
Query: 186 VSYPIIIDQKYCPNNICVKSG 248
SYPIIIDQKYCP+ +C G
Sbjct: 311 SSYPIIIDQKYCPHKLCTAKG 373
>gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_C11 5', mRNA sequence
Length = 647
Score = 91.8 bits (194), Expect(2) = 4e-026
Identities = 37/76 (48%), Positives = 53/76 (69%)
Frame = +3 / +1
Query: 240 KSGASKVAVNDVVFKNIHGTSNTPEAITLNCANNLPCQGVQLINVDIKYNRSDNKTMSVC 419
K +S+V V DV FKNI GTS+TPEA++L C++ PC+GV + +V I+Y+ +NKTM+VC
Sbjct: 64 KGDSSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVC 243
Query: 420 KNAIGKSIGMAKELAC 467
N + G+ K AC
Sbjct: 244 TNVKVTATGVDKANAC 291
Score = 46.0 bits (94), Expect(2) = 4e-026
Identities = 16/25 (64%), Positives = 18/25 (72%)
Frame = +3 / +1
Query: 180 DNVSYPIIIDQKYCPNNICVKSGAS 254
D+ YPIIIDQKYCPN +C G S
Sbjct: 1 DDSGYPIIIDQKYCPNKLCTAKGDS 75
>gb|AL506753.1|AL506753 AL506753 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04C10T 5',
mRNA sequence
Length = 507
Score = 74.8 bits (157), Expect(2) = 1e-021
Identities = 29/73 (39%), Positives = 49/73 (67%)
Frame = +3 / +2
Query: 162 YQDVTMDNVSYPIIIDQKYCPNNICVKSGASKVAVNDVVFKNIHGTSNTPEAITLNCANN 341
+ +V +D V +PIIIDQ YC + + ++ VAV++V+++NI GTS EAI L C++
Sbjct: 167 FTNVLVDGVDHPIIIDQFYCDSRTPCANRSTNVAVSNVMYRNISGTSTRDEAIKLACSDA 346
Query: 342 LPCQGVQLINVDI 380
+PC + L N+++
Sbjct: 347 VPCSDIVLSNINL 385
Score = 47.8 bits (98), Expect(2) = 1e-021
Identities = 20/52 (38%), Positives = 31/52 (59%)
Frame = +3 / +2
Query: 3 GHGISVGSLGRYKDEKDVEDVKVTGCTLAGTTNGLRIKSYEDSKSSLKATKF 158
GHGIS+GSLG+ VE V + G +A NG+RIK+++ ++ +F
Sbjct: 14 GHGISIGSLGQGGSYAAVEGVTLDGARIARAQNGVRIKTWQGGAGYVRNVRF 169
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 186,679,419
Number of Sequences: 312970
Number of extensions: 1977527
Number of successful extensions: 40183
Number of sequences better than 1.0e-020: 7
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6871506858
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)