TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115147.2.2
(1310 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442182.2|BM442182 EBan01_SQ002_H09_R anther, yellow st... 167 9e-059
gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. v... 92 4e-041
gb|CA022678.1|CA022678 HZ44A18r HZ Hordeum vulgare subsp. v... 55 3e-022
gb|CA022603.1|CA022603 HZ43M20r HZ Hordeum vulgare subsp. v... 55 3e-022
gb|BI950366.1|BI950366 HVSMEl0020G12f Hordeum vulgare spike... 67 4e-022
>gb|BM442182.2|BM442182 EBan01_SQ002_H09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_H09 5', mRNA sequence
Length = 687
Score = 167 bits (359), Expect(2) = 9e-059
Identities = 61/82 (74%), Positives = 74/82 (90%)
Frame = +1 / +2
Query: 826 IEGAIDTGFSFKNCSIGGVKGGQIYLGRAWGDSSRVVYSYTKMGEEVVPVGWDGWQIAKP 1005
IEGAI++GFSFKNC+I GG IYLGRAWGDSSRV+Y+YT+M +EVVPVGWDGW++ +P
Sbjct: 2 IEGAIESGFSFKNCTIMSEGGGDIYLGRAWGDSSRVIYAYTEMSKEVVPVGWDGWEVKQP 181
Query: 1006 ESSGIYYGEFKCFGPGADAKKK 1071
ESSGIYYGEFKC GPG+DA+K+
Sbjct: 182 ESSGIYYGEFKCSGPGSDARKR 247
Score = 116 bits (249), Expect(2) = 2e-038
Identities = 55/81 (67%), Positives = 61/81 (75%)
Frame = -1 / -1
Query: 1067 FLASAPGPKHLNSP**MPLLSGLAICQPSQPTGTTSSPIFVYE*TTREESPQARPR*ICP 888
FLAS PGP HLNSP**+PLLSG PS PTGTTS I V * TR+ESP A PR*I P
Sbjct: 243 FLASDPGPLHLNSP**IPLLSGCLTSHPSHPTGTTSLLISV*A*MTRDESPHALPR*ISP 64
Query: 887 PLTPPMLQFLNEKPVSMAPSM 825
P + M+QFLNEKP+S+APSM
Sbjct: 63 PPSLMMVQFLNEKPLSIAPSM 1
Score = 80.8 bits (170), Expect(2) = 9e-059
Identities = 29/40 (72%), Positives = 34/40 (85%)
Frame = +1 / +2
Query: 1051 GADAKKKKRVGWALDLTEAQAKPFVGTHYVLGDTWIQPPP 1170
G + +KRVGWA+DLTEAQAKPF+GTHYV GD+WI PPP
Sbjct: 221 GPGSDARKRVGWAVDLTEAQAKPFMGTHYVFGDSWILPPP 340
Score = 62.9 bits (131), Expect(2) = 2e-038
Identities = 27/38 (71%), Positives = 30/38 (78%)
Frame = -1 / -1
Query: 1184 GYFYLGGGWIHVSPRT*CVPTNGLACASVRSSAHPTLF 1071
G F +GGG I SP+T*CVP GLA ASVRS+AHPTLF
Sbjct: 354 GEFPVGGGRIQESPKT*CVPMKGLAWASVRSTAHPTLF 241
>gb|CA029858.1|CA029858 HX05G15r HX Hordeum vulgare subsp. vulgare cDNA clone HX05G15
5-PRIME, mRNA sequence
Length = 638
Score = 92.3 bits (195), Expect(5) = 4e-041
Identities = 37/85 (43%), Positives = 45/85 (52%)
Frame = +1 / +1
Query: 577 GAKGGQAVAVRLFGTKTQIYNCTIDGGQDTLYDHKGLHYFKGCLIRGSVDFIFGFGRSFY 756
G G VA+R+ C G QDTLYDH G HY+K C I GSVDFIFG S Y
Sbjct: 4 GRAGEAGVALRISADNAAFVGCNFLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLY 183
Query: 757 EDCRIESVVKEVAVLTAQQRSKSIE 831
E C + ++ LTAQ R ++
Sbjct: 184 EGCHVHAISPHYGALTAQNRRSMLD 258
Score = 55.1 bits (114), Expect(5) = 4e-041
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = +1 / +1
Query: 889 GQIYLGRAWGDSSRVVYSYTKMGEEVVPVGWDGW 990
G +YLGRAWG SRVV++YT M ++P GW W
Sbjct: 301 GALYLGRAWGTFSRVVFAYTYMDNIIIPRGWYNW 402
Score = 37.7 bits (76), Expect(5) = 4e-041
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1 / +1
Query: 1051 GADAKKKKRVGWALDLTEAQAKPFVGTHYVLGDTWIQ 1161
G A RV W+ +LT+ +AKPF+ ++ G W++
Sbjct: 454 GPGANHAGRVDWSRELTDEEAKPFISLSFIDGLEWLR 564
Score = 30.4 bits (60), Expect(5) = 4e-041
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +1 / +1
Query: 1018 IYYGEFKCFGPGAD 1059
++YG++KC GPGA+
Sbjct: 427 VFYGQYKCSGPGAN 468
Score = 26.7 bits (52), Expect(5) = 4e-041
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +1 / +1
Query: 841 DTGFSFKNCSIGG 879
DTGFSF NC + G
Sbjct: 259 DTGFSFLNCRVTG 297
>gb|CA022678.1|CA022678 HZ44A18r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ44A18
5-PRIME, mRNA sequence
Length = 546
Score = 55.1 bits (114), Expect(5) = 3e-022
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = +1 / +3
Query: 889 GQIYLGRAWGDSSRVVYSYTKMGEEVVPVGWDGW 990
G +YLGRAWG SRVV++YT M ++P GW W
Sbjct: 144 GALYLGRAWGTFSRVVFAYTYMDNIIIPRGWYNW 245
Score = 37.7 bits (76), Expect(5) = 3e-022
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1 / +3
Query: 1051 GADAKKKKRVGWALDLTEAQAKPFVGTHYVLGDTWIQ 1161
G A RV W+ +LT+ +AKPF+ ++ G W++
Sbjct: 297 GPGANHAGRVDWSRELTDEEAKPFISLSFIDGLEWLR 407
Score = 30.4 bits (60), Expect(5) = 3e-022
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +1 / +3
Query: 1018 IYYGEFKCFGPGAD 1059
++YG++KC GPGA+
Sbjct: 270 VFYGQYKCSGPGAN 311
Score = 26.7 bits (52), Expect(5) = 3e-022
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +1 / +3
Query: 841 DTGFSFKNCSIGG 879
DTGFSF NC + G
Sbjct: 102 DTGFSFLNCRVTG 140
Score = 26.7 bits (52), Expect(5) = 3e-022
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = +1 / +3
Query: 748 SFYEDCRIESVVKEVAVLTAQQRSKSIE 831
S YE C + ++ LTAQ R ++
Sbjct: 18 SLYEGCHVHAISPHYGALTAQNRRSMLD 101
>gb|CA022603.1|CA022603 HZ43M20r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ43M20
5-PRIME, mRNA sequence
Length = 450
Score = 55.1 bits (114), Expect(5) = 3e-022
Identities = 19/34 (55%), Positives = 24/34 (70%)
Frame = +1 / +2
Query: 889 GQIYLGRAWGDSSRVVYSYTKMGEEVVPVGWDGW 990
G +YLGRAWG SRVV++YT M ++P GW W
Sbjct: 143 GALYLGRAWGTFSRVVFAYTYMDNIIIPRGWYNW 244
Score = 37.7 bits (76), Expect(5) = 3e-022
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1 / +2
Query: 1051 GADAKKKKRVGWALDLTEAQAKPFVGTHYVLGDTWIQ 1161
G A RV W+ +LT+ +AKPF+ ++ G W++
Sbjct: 296 GPGANHAGRVDWSRELTDEEAKPFISLSFIDGLEWLR 406
Score = 30.4 bits (60), Expect(5) = 3e-022
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +1 / +2
Query: 1018 IYYGEFKCFGPGAD 1059
++YG++KC GPGA+
Sbjct: 269 VFYGQYKCSGPGAN 310
Score = 26.7 bits (52), Expect(5) = 3e-022
Identities = 9/13 (69%), Positives = 10/13 (76%)
Frame = +1 / +2
Query: 841 DTGFSFKNCSIGG 879
DTGFSF NC + G
Sbjct: 101 DTGFSFLNCRVTG 139
Score = 26.7 bits (52), Expect(5) = 3e-022
Identities = 9/28 (32%), Positives = 14/28 (50%)
Frame = +1 / +2
Query: 748 SFYEDCRIESVVKEVAVLTAQQRSKSIE 831
S YE C + ++ LTAQ R ++
Sbjct: 17 SLYEGCHVHAISPHYGALTAQNRRSMLD 100
>gb|BI950366.1|BI950366 HVSMEl0020G12f Hordeum vulgare spike EST library HVcDNA0012 (Fusarium
infected) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEl0020G12f, mRNA sequence
Length = 904
Score = 66.6 bits (139), Expect(3) = 4e-022
Identities = 27/60 (45%), Positives = 32/60 (53%)
Frame = +1 / +3
Query: 844 TGFSFKNCSIGGVKGGQIYLGRAWGDSSRVVYSYTKMGEEVVPVGWDGWQIAKPESSGIY 1023
TG+SF C + GV G LGR WG SRVV++ T M V P GWD W E + Y
Sbjct: 138 TGYSFVRCKLTGVGAGTSILGRPWGQYSRVVFALTDMSAAVNPRGWDHWNNTSKERTVFY 317
Score = 42.8 bits (87), Expect(3) = 4e-022
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +1 / +3
Query: 700 GCLIRGSVDFIFGFGRSFYEDCRIESVVKEVAVLTAQQRSK 822
GC + G DFI G GR+ +E C + S TAQ+ S+
Sbjct: 6 GCYVEGGTDFICGNGRALFEKCHLHSTSPNGGAFTAQRASE 128
Score = 33.6 bits (67), Expect(3) = 4e-022
Identities = 13/37 (35%), Positives = 22/37 (59%)
Frame = +1 / +3
Query: 1051 GADAKKKKRVGWALDLTEAQAKPFVGTHYVLGDTWIQ 1161
G +K RV WA +L++A+A PF+ + G W++
Sbjct: 336 GEGSKTDGRVKWARNLSKAEAAPFMTKARIGGQQWLR 446
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 462,936,074
Number of Sequences: 312970
Number of extensions: 7828831
Number of successful extensions: 88702
Number of sequences better than 1.0e-020: 5
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 16005864527
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)