TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3115079.2.1
         (636 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AH014393.1|SEG_AY643842S  Hordeum vulgare subsp. vulgare ...    83   1e-024
gb|AY643843.1|AY643842S2  Hordeum vulgare subsp. vulgare clo...    83   1e-024

>gb|AH014393.1|SEG_AY643842S Hordeum vulgare subsp. vulgare clones BAC 519K7, 799C8, and 122A5
              hardness locus region
          Length = 302994

 Score = 82.6 bits (174), Expect(2) = 1e-024
 Identities = 34/66 (51%), Positives = 48/66 (72%)
 Frame = -2 / -3

                                                                          
Query: 563    FNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYLRDELK 384
              ++ +FWAVHPGGPAIL ++E  L L   KL+ SR AL ++GN SSNTI YVLE + +E +
Sbjct: 138184 YDKMFWAVHPGGPAILTKMEGRLGLDGGKLRASRSALRDFGNASSNTIVYVLENMVEESR 138005

                    
Query: 383    KGAISE 366
              +  ++E
Sbjct: 138004 RQRMTE 137987




 Score = 49.6 bits (102), Expect(2) = 1e-024
 Identities = 18/23 (78%), Positives = 21/23 (91%)
 Frame = -2 / -3

                                     
Query: 365    EWGLILAFGPGITFEGLLVRGVK 297
              EWGLILAFGPGITFEG+L R ++
Sbjct: 137911 EWGLILAFGPGITFEGILARNLQ 137843



>gb|AY643843.1|AY643842S2 Hordeum vulgare subsp. vulgare clones BAC 519K7 and 799C8 hardness locus
              region
          Length = 129099

 Score = 82.6 bits (174), Expect(2) = 1e-024
 Identities = 34/66 (51%), Positives = 48/66 (72%)
 Frame = -2 / -2

                                                                          
Query: 563    FNDIFWAVHPGGPAILNRLEFCLKLQPEKLKISRKALMNYGNVSSNTIFYVLEYLRDELK 384
              ++ +FWAVHPGGPAIL ++E  L L   KL+ SR AL ++GN SSNTI YVLE + +E +
Sbjct: 125186 YDKMFWAVHPGGPAILTKMEGRLGLDGGKLRASRSALRDFGNASSNTIVYVLENMVEESR 125007

                    
Query: 383    KGAISE 366
              +  ++E
Sbjct: 125006 RQRMTE 124989




 Score = 49.6 bits (102), Expect(2) = 1e-024
 Identities = 18/23 (78%), Positives = 21/23 (91%)
 Frame = -2 / -2

                                     
Query: 365    EWGLILAFGPGITFEGLLVRGVK 297
              EWGLILAFGPGITFEG+L R ++
Sbjct: 124913 EWGLILAFGPGITFEGILARNLQ 124845



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 288,071,452
Number of Sequences: 312970
Number of extensions: 4052018
Number of successful extensions: 36273
Number of sequences better than 1.0e-020: 2
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6786673440
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)