TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071761.2.1
(638 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. v... 222 1e-057
gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedl... 222 2e-057
gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachi... 176 5e-054
gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 ... 203 1e-051
gb|AV833853.1|AV833853 AV833853 K. Sato unpublished cDNA li... 163 4e-049
gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hyd... 187 6e-047
gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke devel... 180 8e-045
gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgar... 160 9e-039
gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedl... 158 2e-038
gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa... 158 2e-038
gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedl... 147 2e-037
gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. v... 147 7e-035
gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA li... 129 2e-033
gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, dro... 138 2e-032
gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA li... 133 9e-031
gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots... 101 2e-028
gb|BQ757736.1|BQ757736 EBem10_SQ005_B05_R embryo, 2 Day ger... 108 3e-023
gb|CV059902.1|CV059902 BNEL52b8 Barley EST endosperm librar... 105 2e-022
gb|CV061694.1|CV061694 BNEL70h10 Barley EST endosperm libra... 105 2e-022
gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke devel... 101 3e-021
>gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. vulgare cDNA clone HL01L15
3-PRIME, mRNA sequence
Length = 490
Score = 222 bits (480), Expect = 1e-057
Identities = 92/110 (83%), Positives = 104/110 (94%)
Frame = +2 / +1
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDRLKEIIKYKGFQVPPAELEALLITH EIKDAAVVSM D+L GE+PVAFIVR EGS+
Sbjct: 61 FIVDRLKEIIKYKGFQVPPAELEALLITHAEIKDAAVVSMQDELTGEVPVAFIVRIEGSE 240
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGV 331
++E EIKQFVAKEVVFYK+IHKVFF +S+PK+P+GKILRKDLRA+LAAG+
Sbjct: 241 ISESEIKQFVAKEVVFYKRIHKVFFADSVPKSPAGKILRKDLRAKLAAGI 390
Score = 190 bits (410), Expect = 5e-048
Identities = 85/109 (77%), Positives = 90/109 (82%)
Frame = -3 / -3
Query: 327 PAASLALKSFLRILPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKAT 148
PAAS AL+SFLRILP G G +S KKTLWI L*KTTS ATNCLIS S+ EPS+RTM AT
Sbjct: 386 PAASFALRSFLRILPAGLFGTESAKKTLWILL*KTTSFATNCLISLSLISEPSIRTMNAT 207
Query: 147 GISPARSSFIDTTAASLISGCVMRSASSSAGGTWKPLYLMISLSLSTMK 1
G SP SS IDTTAASLIS CVMR ASSSAGGTW PLYL+ISLSLSTMK
Sbjct: 206 GTSPVSSSCIDTTAASLISACVMRRASSSAGGTWNPLYLIISLSLSTMK 60
>gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0001H21f, mRNA sequence
Length = 922
Score = 222 bits (479), Expect = 2e-057
Identities = 90/110 (81%), Positives = 105/110 (95%)
Frame = +2 / +1
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDRLKEI+KYKGFQV PAELEALLITHPEIKDAAVVS+ DDLAGE+P+AF++R EGS+
Sbjct: 154 FIVDRLKEIMKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSE 333
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLAAGV 331
+TED+IK+FVAKEV FYK+IHKVFFT+S+PKNPSG+ILRKDLRARLA G+
Sbjct: 334 ITEDDIKKFVAKEVGFYKRIHKVFFTDSMPKNPSGEILRKDLRARLATGI 483
Score = 190 bits (409), Expect = 7e-048
Identities = 87/109 (79%), Positives = 92/109 (84%)
Frame = -3 / -3
Query: 327 PAASLALKSFLRILPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKAT 148
P ASLALKSFLRI P+GFLG++SVKKTLWI L*K TS ATN L+SSSV EPS+R KA
Sbjct: 479 PVASLALKSFLRISPEGFLGIESVKKTLWILL*KPTSFATNFLMSSSVISEPSIRITKAI 300
Query: 147 GISPARSSFIDTTAASLISGCVMRSASSSAGGTWKPLYLMISLSLSTMK 1
G SPARSSF DTTAASLISG VMR ASSSAG TWKPLY MISLSLSTMK
Sbjct: 299 GTSPARSSFSDTTAASLISGWVMRRASSSAGATWKPLYFMISLSLSTMK 153
Score = 127 bits (271), Expect(2) = 5e-032
Identities = 54/76 (71%), Positives = 60/76 (78%)
Frame = +1 / +3
Query: 94 DQGRRRRINER*PCW*NPGRLHRADRRFSSHRG*DQAIRRQGGGFLQEDPQGLLHRIHPQ 273
DQGRRRRI E CW +P RL ADRR +HRG* Q IRR+GG FLQEDPQGLLHR++ Q
Sbjct: 246 DQGRRRRIAEGRSCWRSPDRLRYADRRL*NHRG*HQKIRREGGWFLQEDPQGLLHRLNAQ 425
Query: 274 EPVGQDPEEGLESQAR 321
EP +DPEEGLESQAR
Sbjct: 426 EPFRRDPEEGLESQAR 473
Score = 30.4 bits (60), Expect(2) = 5e-032
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = +1 / +3
Query: 1 LHRRQAQGDHQVQGL 45
LH QAQGDH+VQGL
Sbjct: 153 LHC*QAQGDHEVQGL 197
>gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0021P10f, mRNA sequence
Length = 657
Score = 176 bits (379), Expect(2) = 5e-054
Identities = 70/84 (83%), Positives = 82/84 (97%)
Frame = +2 / -1
Query: 80 ITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFT 259
ITHPEIKDAAVVS+ DDLAGE+P+AF++R EGS++TED+IK+FVAKEVVFYK+IHKVFFT
Sbjct: 552 ITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFT 373
Query: 260 ESIPKNPSGKILRKDLRARLAAGV 331
+SIPKNPSGKILRKDLRARLAAG+
Sbjct: 372 DSIPKNPSGKILRKDLRARLAAGI 301
Score = 145 bits (312), Expect(3) = 9e-046
Identities = 67/83 (80%), Positives = 72/83 (86%)
Frame = -3 / +2
Query: 327 PAASLALKSFLRILPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKAT 148
PAASLALKSFLRILP+GFLG++SVKKTLWI L*KTTS ATN L+SSSV EPS+R KA
Sbjct: 305 PAASLALKSFLRILPEGFLGIESVKKTLWILL*KTTSFATNFLMSSSVISEPSIRITKAI 484
Query: 147 GISPARSSFIDTTAASLISGCVM 79
G SPARSSF DTTAASLISG VM
Sbjct: 485 GTSPARSSFSDTTAASLISGWVM 553
Score = 134 bits (287), Expect(2) = 1e-034
Identities = 57/77 (74%), Positives = 62/77 (80%)
Frame = +1 / -3
Query: 94 DQGRRRRINER*PCW*NPGRLHRADRRFSSHRG*DQAIRRQGGGFLQEDPQGLLHRIHPQ 273
DQGRRRRI E CW +P RL ADRR +HRG* Q IRR+GG FLQEDPQGLLHR++PQ
Sbjct: 538 DQGRRRRIAEGRSCWRSPDRLRYADRRL*NHRG*HQKIRREGGCFLQEDPQGLLHRLNPQ 359
Query: 274 EPVGQDPEEGLESQARR 324
EP QDPEEGLESQARR
Sbjct: 358 EPFRQDPEEGLESQARR 308
Score = 105 bits (225), Expect(3) = 2e-024
Identities = 47/81 (58%), Positives = 53/81 (65%)
Frame = -2 / +1
Query: 343 RASVNTGGEPGSQVLPQDLARRVLGDGFGEEDLVDLLVENHXXXXXXXXXXXXXLRTFGP 164
R + GEPGSQVLPQDLA RVLGD GEEDLVD LVEN+ R F P
Sbjct: 289 RLXGDASGEPGSQVLPQDLA*RVLGD*VGEEDLVDPLVENNLLRDEFFDVILGDFRAFDP 468
Query: 163 HDEGDRDFTSKVIVH*YDGGV 101
H+EGDRDF SK+++ YDGGV
Sbjct: 469 HNEGDRDFASKIVLQRYDGGV 531
Score = 54.7 bits (113), Expect(3) = 9e-046
Identities = 23/24 (95%), Positives = 23/24 (95%)
Frame = -3 / +3
Query: 72 ASSSAGGTWKPLYLMISLSLSTMK 1
ASSSAG TWKPLYLMISLSLSTMK
Sbjct: 561 ASSSAGATWKPLYLMISLSLSTMK 632
Score = 54.7 bits (113), Expect(2) = 5e-054
Identities = 23/24 (95%), Positives = 23/24 (95%)
Frame = +2 / -3
Query: 2 FIVDRLKEIIKYKGFQVPPAELEA 73
FIVDRLKEIIKYKGFQV PAELEA
Sbjct: 631 FIVDRLKEIIKYKGFQVAPAELEA 560
Score = 31.8 bits (63), Expect(2) = 1e-034
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / -2
Query: 1 LHRRQAQGDHQVQGL 45
LH QAQGDHQVQGL
Sbjct: 632 LHC*QAQGDHQVQGL 588
Score = 23.5 bits (45), Expect(3) = 9e-046
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -3 / +2
Query: 573 NQACSLGQKQH 541
NQ CS QKQH
Sbjct: 95 NQPCSYAQKQH 127
Score = 23.1 bits (44), Expect(3) = 2e-024
Identities = 10/12 (83%), Positives = 12/12 (100%)
Frame = -1 / +1
Query: 38 CT**SP*ACRR* 3
CT**SP*AC++*
Sbjct: 595 CT**SP*ACQQ* 630
Score = 22.6 bits (43), Expect(3) = 2e-024
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -1 / +3
Query: 566 LVLWVKNNIETTSISF 519
LVL +KNN T ISF
Sbjct: 102 LVLMLKNNTSTYIISF 149
>gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0094P16f, mRNA sequence
Length = 685
Score = 203 bits (437), Expect = 1e-051
Identities = 82/96 (85%), Positives = 93/96 (96%)
Frame = +2 / +1
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDRLKEIIKYKGFQV PAELEALLITHPEIKDAAVVS+ DDLAGE+P+AF++R EGS+
Sbjct: 394 FIVDRLKEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSE 573
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGK 289
+TED+IK+FVAKEVVFYK+IHKVFFT+SIPKNPSGK
Sbjct: 574 ITEDDIKKFVAKEVVFYKRIHKVFFTDSIPKNPSGK 681
Score = 172 bits (370), Expect = 2e-042
Identities = 78/96 (81%), Positives = 83/96 (86%)
Frame = -3 / -3
Query: 288 LPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKATGISPARSSFIDTT 109
LP+GFLG++SVKKTLWI L*KTTS ATN L+SSSV EPS+R KA G SPARSSF DTT
Sbjct: 680 LPEGFLGIESVKKTLWILL*KTTSFATNFLMSSSVISEPSIRITKAIGTSPARSSFSDTT 501
Query: 108 AASLISGCVMRSASSSAGGTWKPLYLMISLSLSTMK 1
AASLISG VMR ASSSAG TWKPLYLMISLSLSTMK
Sbjct: 500 AASLISGWVMRRASSSAGATWKPLYLMISLSLSTMK 393
Score = 100 bits (214), Expect(2) = 1e-024
Identities = 43/65 (66%), Positives = 48/65 (73%)
Frame = +1 / +3
Query: 94 DQGRRRRINER*PCW*NPGRLHRADRRFSSHRG*DQAIRRQGGGFLQEDPQGLLHRIHPQ 273
DQGRRRRI E CW +P RL ADRR +HRG* Q IRR+ G FLQEDPQGL HR++PQ
Sbjct: 486 DQGRRRRIAEGRSCWRSPDRLRYADRRL*NHRG*HQKIRRERGCFLQEDPQGLFHRLNPQ 665
Query: 274 EPVGQ 288
EP Q
Sbjct: 666 EPFRQ 680
Score = 31.8 bits (63), Expect(2) = 1e-024
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / +3
Query: 1 LHRRQAQGDHQVQGL 45
LH QAQGDHQVQGL
Sbjct: 393 LHC*QAQGDHQVQGL 437
>gb|AV833853.1|AV833853 AV833853 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags8m01, mRNA sequence
Length = 599
Score = 163 bits (350), Expect(3) = 4e-049
Identities = 65/79 (82%), Positives = 77/79 (97%)
Frame = +2 / +3
Query: 95 IKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFTESIPK 274
IKDAAVVS+ DDLAGE+P+AF++R EGS++TED+IK+FVAKEVVFYK+IHKVFFT+SIPK
Sbjct: 168 IKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFTDSIPK 347
Query: 275 NPSGKILRKDLRARLAAGV 331
NPSGKILRKDLRARLAAG+
Sbjct: 348 NPSGKILRKDLRARLAAGI 404
Score = 136 bits (291), Expect(2) = 8e-040
Identities = 62/77 (80%), Positives = 67/77 (87%)
Frame = -3 / -2
Query: 327 PAASLALKSFLRILPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKAT 148
PAASLALKSFLRILP+GFLG++SVKKTLWI L*KTTS ATN L+SSSV EPS+R KA
Sbjct: 400 PAASLALKSFLRILPEGFLGIESVKKTLWILL*KTTSFATNFLMSSSVISEPSIRITKAI 221
Query: 147 GISPARSSFIDTTAASL 97
G SPARSSF DTTAASL
Sbjct: 220 GTSPARSSFSDTTAASL 170
Score = 131 bits (281), Expect(2) = 8e-034
Identities = 56/76 (73%), Positives = 61/76 (80%)
Frame = +1 / +2
Query: 97 QGRRRRINER*PCW*NPGRLHRADRRFSSHRG*DQAIRRQGGGFLQEDPQGLLHRIHPQE 276
QGRRRRI E CW +P RL ADRR +HRG* Q IRR+GG FLQEDPQGLLHR++PQE
Sbjct: 170 QGRRRRIAEGRSCWRSPDRLRYADRRL*NHRG*HQKIRREGGCFLQEDPQGLLHRLNPQE 349
Query: 277 PVGQDPEEGLESQARR 324
P QDPEEGLESQARR
Sbjct: 350 PFRQDPEEGLESQARR 397
Score = 106 bits (226), Expect(2) = 1e-023
Identities = 47/81 (58%), Positives = 54/81 (66%)
Frame = -2 / -1
Query: 343 RASVNTGGEPGSQVLPQDLARRVLGDGFGEEDLVDLLVENHXXXXXXXXXXXXXLRTFGP 164
R + + GEPGSQVLPQDLA RVLGD GEEDLVD LVEN+ R F P
Sbjct: 416 RLAGDASGEPGSQVLPQDLA*RVLGD*VGEEDLVDPLVENNLLRDEFFDVILGDFRAFDP 237
Query: 163 HDEGDRDFTSKVIVH*YDGGV 101
H+EGDRDF SK+++ YDGGV
Sbjct: 236 HNEGDRDFASKIVLQRYDGGV 174
Score = 47.3 bits (97), Expect(2) = 8e-040
Identities = 19/20 (95%), Positives = 19/20 (95%)
Frame = -3 / -1
Query: 60 AGGTWKPLYLMISLSLSTMK 1
AG TWKPLYLMISLSLSTMK
Sbjct: 131 AGATWKPLYLMISLSLSTMK 72
Score = 46.4 bits (95), Expect(3) = 4e-049
Identities = 19/20 (95%), Positives = 19/20 (95%)
Frame = +2 / +1
Query: 2 FIVDRLKEIIKYKGFQVPPA 61
FIVDRLKEIIKYKGFQV PA
Sbjct: 73 FIVDRLKEIIKYKGFQVAPA 132
Score = 31.8 bits (63), Expect(2) = 8e-034
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / +3
Query: 1 LHRRQAQGDHQVQGL 45
LH QAQGDHQVQGL
Sbjct: 72 LHC*QAQGDHQVQGL 116
Score = 23.5 bits (45), Expect(3) = 4e-049
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2 / +2
Query: 65 LEALLITHP 91
LEALLITHP
Sbjct: 137 LEALLITHP 163
Score = 23.1 bits (44), Expect(2) = 1e-023
Identities = 10/12 (83%), Positives = 12/12 (100%)
Frame = -1 / -2
Query: 38 CT**SP*ACRR* 3
CT**SP*AC++*
Sbjct: 109 CT**SP*ACQQ* 74
>gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_M23 5', mRNA sequence
Length = 590
Score = 187 bits (402), Expect = 6e-047
Identities = 76/91 (83%), Positives = 87/91 (95%)
Frame = +2 / +3
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDRLKEIIKYKGFQV PAELEALLITHPEIKDAAVVS+ DDLAGE+P+AF++R EGS+
Sbjct: 318 FIVDRLKEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSE 497
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPK 274
+TED+IK+FVAKE VFYK+IHKVFFT+SIPK
Sbjct: 498 ITEDDIKKFVAKEGVFYKRIHKVFFTDSIPK 590
Score = 158 bits (339), Expect = 3e-038
Identities = 73/91 (80%), Positives = 77/91 (84%)
Frame = -3 / -2
Query: 273 LGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKATGISPARSSFIDTTAASLI 94
LG++SVKKTLWI L*KT S ATN L+SSSV EPS+R KA G SPARSSF DTTAASLI
Sbjct: 589 LGIESVKKTLWILL*KTPSFATNFLMSSSVISEPSIRITKAIGTSPARSSFSDTTAASLI 410
Query: 93 SGCVMRSASSSAGGTWKPLYLMISLSLSTMK 1
SG VMR ASSSAG TWKPLYLMISLSLSTMK
Sbjct: 409 SGWVMRRASSSAGATWKPLYLMISLSLSTMK 317
Score = 100 bits (212), Expect(2) = 2e-024
Identities = 42/60 (70%), Positives = 47/60 (78%)
Frame = +1 / +2
Query: 94 DQGRRRRINER*PCW*NPGRLHRADRRFSSHRG*DQAIRRQGGGFLQEDPQGLLHRIHPQ 273
DQGRRRRI E CW +P RL ADRR +HRG* Q IRR+GG FLQEDPQGLLHR++PQ
Sbjct: 410 DQGRRRRIAEGRSCWRSPDRLRYADRRL*NHRG*HQKIRREGGCFLQEDPQGLLHRLNPQ 589
Score = 31.8 bits (63), Expect(2) = 2e-024
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +1 / +2
Query: 1 LHRRQAQGDHQVQGL 45
LH QAQGDHQVQGL
Sbjct: 317 LHC*QAQGDHQVQGL 361
>gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21T 5',
mRNA sequence
Length = 700
Score = 180 bits (387), Expect = 8e-045
Identities = 72/106 (67%), Positives = 90/106 (84%)
Frame = +2 / -2
Query: 5 IVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQV 184
IVDR+KE+IK+KGFQVPPAELEA LI HP I DAAVV DD AGE+PVAF+VR S +
Sbjct: 618 IVDRVKELIKFKGFQVPPAELEAXLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADSDI 439
Query: 185 TEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARLA 322
E+ IK+FV+K+VVFYK++HKV+FT +IPK+ SGKILRK+LRA+L+
Sbjct: 438 AEEAIKEFVSKQVVFYKRLHKVYFTHAIPKSASGKILRKELRAKLS 301
Score = 131 bits (280), Expect = 4e-030
Identities = 65/106 (61%), Positives = 71/106 (66%)
Frame = -3 / +2
Query: 318 SLALKSFLRILPDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKATGIS 139
SLAL SFL I PD LG+ VK TL L* TT L TN L++SS E + RT ATG S
Sbjct: 305 SLALSSFLSIFPDADLGIAWVKYTLCSLL*NTTCLDTNSLMASSAMSESAARTTNATGTS 484
Query: 138 PARSSFIDTTAASLISGCVMRSASSSAGGTWKPLYLMISLSLSTMK 1
PA SSF TTAAS + GC M ASSSAGGTWKPL LM SL+ ST K
Sbjct: 485 PAASSFCGTTAASAMEGCAMSXASSSAGGTWKPLNLMSSLTRSTXK 622
>gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K1510 5-PRIME, mRNA sequence
Length = 630
Score = 160 bits (343), Expect = 9e-039
Identities = 63/91 (69%), Positives = 77/91 (84%)
Frame = +2 / +1
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDR+KE+IK+KGFQVPPAELEALLI HP I DAAVV DD AGE+PVAF+VR S
Sbjct: 289 FIVDRVKELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADSD 468
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPK 274
+ E+ IK+FV+K+VVFYK++HKV+FT +IPK
Sbjct: 469 IAEEAIKEFVSKQVVFYKRLHKVYFTHAIPK 561
Score = 114 bits (243), Expect = 6e-025
Identities = 57/95 (60%), Positives = 63/95 (66%)
Frame = -3 / -2
Query: 285 PDGFLGMDSVKKTLWIFL*KTTSLATNCLISSSVT*EPSVRTMKATGISPARSSFIDTTA 106
P LG+ VK TL L* TT L TN L++SS E + RT ATG SPA SSF TTA
Sbjct: 572 PTPNLGIAWVKYTLCSLL*NTTCLDTNSLMASSAMSESAARTTNATGTSPAASSFCGTTA 393
Query: 105 ASLISGCVMRSASSSAGGTWKPLYLMISLSLSTMK 1
AS + GC M ASSSAGGTWKPL LM SL+ STMK
Sbjct: 392 ASAMEGCAMSRASSSAGGTWKPLNLMSSLTRSTMK 288
>gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0021D12f, mRNA sequence
Length = 806
Score = 158 bits (340), Expect = 2e-038
Identities = 63/102 (61%), Positives = 80/102 (78%)
Frame = +2 / +3
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
F+VDRLKE+IKYKG+QVPPAELEALL+THPE+ D AV+ D G+ P+A++VR +GS
Sbjct: 294 FVVDRLKELIKYKGYQVPPAELEALLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSN 473
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDL 307
++ E+ +FVAK+V YKK+ KV F IPKN SGKILRKDL
Sbjct: 474 LSAQEVMEFVAKQVAPYKKVRKVAFVTDIPKNASGKILRKDL 599
>gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0002N03f, mRNA sequence
Length = 876
Score = 158 bits (340), Expect = 2e-038
Identities = 59/106 (55%), Positives = 88/106 (83%)
Frame = +2 / +3
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
F+VDRLKE+IKYKGFQ+ PAELE LL++HPEI DA V+ DD AGE+P+A++VR+ S
Sbjct: 348 FVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASS 527
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARL 319
+TE ++++F+AK+V +YK++ +V F ES+PK+ +GKILR++L A++
Sbjct: 528 LTEVDVQKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRRELIAQV 665
>gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0015K22f, mRNA sequence
Length = 817
Score = 147 bits (315), Expect(2) = 2e-037
Identities = 64/78 (82%), Positives = 69/78 (88%)
Frame = +2 / +3
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDRLKEIIKYKGFQVPPAELE LLITH EIKDAAVVSM D+L GE+PVAFIVR EGS+
Sbjct: 525 FIVDRLKEIIKYKGFQVPPAELEPLLITHAEIKDAAVVSMQDELTGEVPVAFIVRIEGSE 704
Query: 182 VTEDEIKQFVAKEVVFYK 235
++E EIKQF AKEVV K
Sbjct: 705 ISESEIKQFGAKEVVSTK 758
Score = 130 bits (279), Expect = 6e-030
Identities = 58/76 (76%), Positives = 62/76 (81%)
Frame = -3 / -1
Query: 228 KTTSLATNCLISSSVT*EPSVRTMKATGISPARSSFIDTTAASLISGCVMRSASSSAGGT 49
+TTS A NCLIS S+ EPS+RTM ATG SP SS IDTTAASLIS CVMR SSSAGGT
Sbjct: 751 ETTSFAPNCLISLSLISEPSIRTMNATGTSPVSSSCIDTTAASLISACVMRRGSSSAGGT 572
Query: 48 WKPLYLMISLSLSTMK 1
W PLYL+ISLSLSTMK
Sbjct: 571 WNPLYLIISLSLSTMK 524
Score = 28.1 bits (55), Expect(2) = 2e-037
Identities = 8/15 (53%), Positives = 14/15 (93%)
Frame = +2 / +3
Query: 245 KVFFTESIPKNPSGK 289
+VFF +S+PK+P+G+
Sbjct: 765 QVFFADSVPKSPAGR 809
>gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. vulgare cDNA clone HF12A02
5-PRIME, mRNA sequence
Length = 577
Score = 147 bits (315), Expect = 7e-035
Identities = 59/102 (57%), Positives = 80/102 (78%)
Frame = +2 / +1
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
+IVDRLKE+IKYKG+QVPPAELE +L +HPEI DAAV+ D+ G++P+AFIVR GS
Sbjct: 127 YIVDRLKELIKYKGYQVPPAELEHILQSHPEIADAAVIGYPDEDVGQLPMAFIVRQPGSS 306
Query: 182 VTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDL 307
+T ++ +VAK V YKK+ +V F +IPK+P+GKILR++L
Sbjct: 307 LTGKQVMDYVAKHVAPYKKVRRVAFVAAIPKSPAGKILRREL 432
>gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 5', mRNA sequence
Length = 547
Score = 129 bits (276), Expect(2) = 2e-033
Identities = 47/83 (56%), Positives = 69/83 (83%)
Frame = +2 / +2
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
F+VDRLKE+IKYKGFQ+ PAELE LL++HPEI DA V+ DD AGE+P+A++VR+ S
Sbjct: 230 FVVDRLKELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASS 409
Query: 182 VTEDEIKQFVAKEVVFYKKIHKV 250
+TE ++++F+AK+V +YK++ +V
Sbjct: 410 LTEVDVQKFIAKQVTYYKRLRRV 478
Score = 32.7 bits (65), Expect(2) = 2e-033
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = +2 / +3
Query: 254 FTESIPKNPSGKILRKDLRAR 316
F ES+PK+ +GKILR++L A+
Sbjct: 483 FVESVPKSAAGKILRRELIAQ 545
>gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ005_H07 5', mRNA sequence
Length = 552
Score = 138 bits (297), Expect = 2e-032
Identities = 56/68 (82%), Positives = 65/68 (95%)
Frame = +2 / +3
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQ 181
FIVDRLKEIIKYKGFQV PAELEALLITHPEIKDAAVVS+ DDLAGE+P+AF++R EGS+
Sbjct: 348 FIVDRLKEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSE 527
Query: 182 VTEDEIKQ 205
+TED+IK+
Sbjct: 528 ITEDDIKK 551
Score = 118 bits (253), Expect = 2e-026
Identities = 55/67 (82%), Positives = 57/67 (85%)
Frame = -3 / -3
Query: 201 LISSSVT*EPSVRTMKATGISPARSSFIDTTAASLISGCVMRSASSSAGGTWKPLYLMIS 22
L+SSSV EPS+R KA G SPARSSF DTTAASLISG VMR ASSSAG TWKPLYLMIS
Sbjct: 547 LMSSSVISEPSIRITKAIGTSPARSSFSDTTAASLISGWVMRRASSSAGATWKPLYLMIS 368
Query: 21 LSLSTMK 1
LSLSTMK
Sbjct: 367 LSLSTMK 347
>gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 3', mRNA sequence
Length = 664
Score = 133 bits (285), Expect = 9e-031
Identities = 50/100 (50%), Positives = 78/100 (78%)
Frame = +2 / -3
Query: 20 KEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEI 199
K + KGFQ+ PAELE LL++HPEI DA V+ DD AGE+P+A++VR+ S +TE ++
Sbjct: 518 KS*LSTKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDV 339
Query: 200 KQFVAKEVVFYKKIHKVFFTESIPKNPSGKILRKDLRARL 319
++F+AK+V +YK++ +V F ES+PK+ +GKILR++L A++
Sbjct: 338 QKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRRELIAQV 219
>gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07I12u 3', mRNA sequence
Length = 592
Score = 101 bits (216), Expect(2) = 2e-028
Identities = 37/77 (48%), Positives = 61/77 (79%)
Frame = +2 / -1
Query: 89 PEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFTESI 268
PEI DA V+ DD AGE+P+A++VR+ S +TE ++++F+AK+V +YK++ +V F ES+
Sbjct: 520 PEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRVTFVESV 341
Query: 269 PKNPSGKILRKDLRARL 319
PK+ +GKILR++L A++
Sbjct: 340 PKSAAGKILRRELIAQV 290
Score = 43.7 bits (89), Expect(2) = 2e-028
Identities = 16/24 (66%), Positives = 20/24 (83%)
Frame = +2 / -3
Query: 20 KEIIKYKGFQVPPAELEALLITHP 91
KE+IK KGFQ+ PAE E LL++HP
Sbjct: 590 KELIKSKGFQIAPAEXEGLLLSHP 519
>gb|BQ757736.1|BQ757736 EBem10_SQ005_B05_R embryo, 2 Day germination, no treatment, cv
Optic, EBem10 Hordeum vulgare subsp. vulgare cDNA clone
EBem10_SQ005_B05 5', mRNA sequence
Length = 338
Score = 108 bits (230), Expect = 3e-023
Identities = 45/69 (65%), Positives = 56/69 (81%)
Frame = +2 / +2
Query: 101 DAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVFFTESIPKNP 280
+AAVV DD AGE+PVAF+V+ GS+V E +IK FVAK+VV YK+IH+VF +SIPKN
Sbjct: 2 EAAVVGYADDQAGELPVAFVVQRSGSKVHEAQIKDFVAKQVVHYKRIHRVFLVDSIPKNA 181
Query: 281 SGKILRKDL 307
+GKILRKDL
Sbjct: 182 AGKILRKDL 208
>gb|CV059902.1|CV059902 BNEL52b8 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL52b8 5' similar to Putative
AMP-binding protein, mRNA sequence
Length = 548
Score = 105 bits (225), Expect = 2e-022
Identities = 42/78 (53%), Positives = 56/78 (71%)
Frame = +2 / +1
Query: 74 LLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVF 253
LL+THPE+ D AV+ D G+ P+A++VR +GS ++ E+ +FVAK+V YKK+ KV
Sbjct: 1 LLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVA 180
Query: 254 FTESIPKNPSGKILRKDL 307
F IPKN SGKILRKDL
Sbjct: 181 FVTDIPKNASGKILRKDL 234
>gb|CV061694.1|CV061694 BNEL70h10 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL70h10 5' similar to Putative
AMP-binding protein, mRNA sequence
Length = 568
Score = 105 bits (225), Expect = 2e-022
Identities = 42/78 (53%), Positives = 56/78 (71%)
Frame = +2 / +1
Query: 74 LLITHPEIKDAAVVSMNDDLAGEIPVAFIVRTEGSQVTEDEIKQFVAKEVVFYKKIHKVF 253
LL+THPE+ D AV+ D G+ P+A++VR +GS ++ E+ +FVAK+V YKK+ KV
Sbjct: 1 LLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEVMEFVAKQVAPYKKVRKVA 180
Query: 254 FTESIPKNPSGKILRKDL 307
F IPKN SGKILRKDL
Sbjct: 181 FVTDIPKNASGKILRKDL 234
>gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21u 3',
mRNA sequence
Length = 508
Score = 101 bits (216), Expect = 3e-021
Identities = 41/53 (77%), Positives = 46/53 (86%)
Frame = +2 / +1
Query: 2 FIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMNDDLAGEIPVAFI 160
FIVDR+KE+IK+KGFQVPPAELEALLI HP I DAAVV DD AGE+PVAF+
Sbjct: 340 FIVDRVKELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEVPVAFV 498
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 282,338,598
Number of Sequences: 312970
Number of extensions: 4644092
Number of successful extensions: 87630
Number of sequences better than 1.0e-020: 26
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6829090149
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)