TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071743.2.1
(586 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF621611.1|BF621611 HVSMEa0011J15f Hordeum vulgare seedl... 110 7e-024
gb|BM817109.1|BM817109 HC02A08_T3.ab1 HC Hordeum vulgare su... 108 3e-023
gb|AL506334.1|AL506334 AL506334 Hordeum vulgare Barke devel... 107 7e-023
gb|BJ470706.1|BJ470706 BJ470706 K. Sato unpublished cDNA li... 107 7e-023
gb|BJ473004.1|BJ473004 BJ473004 K. Sato unpublished cDNA li... 107 7e-023
gb|BQ765720.1|BQ765720 EBro03_SQ008_A02_R root, 3 week, wat... 107 7e-023
gb|BU998550.1|BU998550 HI11G16r HI Hordeum vulgare subsp. v... 107 7e-023
gb|BF627268.3|BF627268 HVSMEb0004G12f Hordeum vulgare seedl... 104 3e-022
gb|BU997001.1|BU997001 HI06J07r HI Hordeum vulgare subsp. v... 104 4e-022
gb|BF625895.2|BF625895 HVSMEa0014B15f Hordeum vulgare seedl... 102 2e-021
gb|CA032790.1|CA032790 HX14D10r HX Hordeum vulgare subsp. v... 101 3e-021
gb|BE558795.1|BE558795 HV_CEb0020G12f Hordeum vulgare seedl... 100 5e-021
gb|BQ758546.1|BQ758546 EBma07_SQ002_G03_R maternal, 21 DPA,... 100 5e-021
gb|BQ758625.1|BQ758625 EBma07_SQ002_G09_R maternal, 21 DPA,... 100 5e-021
gb|BQ758717.1|BQ758717 EBma07_SQ002_I14_R maternal, 21 DPA,... 100 5e-021
gb|BQ760943.1|BQ760943 EBro03_SQ003_O16_R root, 3 week, wat... 100 5e-021
gb|CA008548.1|CA008548 HU11E24r HU Hordeum vulgare subsp. v... 100 5e-021
gb|CA025237.1|CA025237 HZ51O06r HZ Hordeum vulgare subsp. v... 100 5e-021
gb|CA030194.1|CA030194 HX06F18r HX Hordeum vulgare subsp. v... 100 5e-021
>gb|BF621611.1|BF621611 HVSMEa0011J15f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011J15f, mRNA sequence
Length = 868
Score = 110 bits (237), Expect = 7e-024
Identities = 46/60 (76%), Positives = 52/60 (86%)
Frame = +1 / +2
Query: 406 TANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
+A K R+RWT ELHE FV++V KL G EKATPKGVLKLMKV+GLTIYHVKSHLQKYR A+
Sbjct: 32 SAAKQRMRWTPELHECFVDSVNKLGGSEKATPKGVLKLMKVDGLTIYHVKSHLQKYRTAR 211
>gb|BM817109.1|BM817109 HC02A08_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC02A08_T3.ab1 similar to (AF219972) CDPK substrate
protein 1; CSP1 [Mesembryanthemum crystallinum], mRNA
sequence
Length = 828
Score = 108 bits (233), Expect = 3e-023
Identities = 45/60 (75%), Positives = 50/60 (83%)
Frame = +1 / +1
Query: 406 TANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
T KSR+RWT ELHE+FV+AV KL G EKATPK V K+MKVEGLTIYHVKSHLQKYR +
Sbjct: 151 TPTKSRMRWTPELHEKFVDAVDKLGGSEKATPKAVQKVMKVEGLTIYHVKSHLQKYRTVR 330
>gb|AL506334.1|AL506334 AL506334 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY02M13T 5',
mRNA sequence
Length = 700
Score = 107 bits (230), Expect = 7e-023
Identities = 44/59 (74%), Positives = 51/59 (86%)
Frame = +1 / +3
Query: 409 ANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
A + RLRWT ELHE+FVEAV +L GP++ATPKGVL++M GLTIYHVKSHLQKYRLAK
Sbjct: 348 AARQRLRWTDELHEQFVEAVTQLGGPDRATPKGVLRIMGTPGLTIYHVKSHLQKYRLAK 524
>gb|BJ470706.1|BJ470706 BJ470706 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal26b02 5', mRNA sequence
Length = 537
Score = 107 bits (230), Expect = 7e-023
Identities = 44/59 (74%), Positives = 51/59 (86%)
Frame = +1 / +1
Query: 409 ANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
A + RLRWT ELHE+FVEAV +L GP++ATPKGVL++M GLTIYHVKSHLQKYRLAK
Sbjct: 256 AARQRLRWTDELHEQFVEAVTQLGGPDRATPKGVLRIMGTPGLTIYHVKSHLQKYRLAK 432
>gb|BJ473004.1|BJ473004 BJ473004 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40f15 5', mRNA sequence
Length = 499
Score = 107 bits (230), Expect = 7e-023
Identities = 44/59 (74%), Positives = 51/59 (86%)
Frame = +1 / +3
Query: 409 ANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
A + RLRWT ELHE+FVEAV +L GP++ATPKGVL++M GLTIYHVKSHLQKYRLAK
Sbjct: 243 AARQRLRWTDELHEQFVEAVTQLGGPDRATPKGVLRIMGTPGLTIYHVKSHLQKYRLAK 419
>gb|BQ765720.1|BQ765720 EBro03_SQ008_A02_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ008_A02 5', mRNA sequence
Length = 626
Score = 107 bits (230), Expect = 7e-023
Identities = 44/59 (74%), Positives = 51/59 (86%)
Frame = +1 / +1
Query: 409 ANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
A + RLRWT ELHE+FVEAV +L GP++ATPKGVL++M GLTIYHVKSHLQKYRLAK
Sbjct: 268 AARQRLRWTDELHEQFVEAVTQLGGPDRATPKGVLRIMGTPGLTIYHVKSHLQKYRLAK 444
>gb|BU998550.1|BU998550 HI11G16r HI Hordeum vulgare subsp. vulgare cDNA clone HI11G16
5-PRIME, mRNA sequence
Length = 625
Score = 107 bits (230), Expect = 7e-023
Identities = 44/59 (74%), Positives = 51/59 (86%)
Frame = +1 / +1
Query: 409 ANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
A + RLRWT ELHE+FVEAV +L GP++ATPKGVL++M GLTIYHVKSHLQKYRLAK
Sbjct: 268 AARQRLRWTDELHEQFVEAVTQLGGPDRATPKGVLRIMGTPGLTIYHVKSHLQKYRLAK 444
>gb|BF627268.3|BF627268 HVSMEb0004G12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0004G12f, mRNA sequence
Length = 875
Score = 104 bits (225), Expect = 3e-022
Identities = 43/58 (74%), Positives = 50/58 (86%)
Frame = +1 / +1
Query: 409 ANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLA 582
A + RLRWT ELHE+FVEAV +L GP++ATPKGVL++M GLTIYHVKSHLQKYRLA
Sbjct: 292 AARQRLRWTDELHEQFVEAVTQLGGPDRATPKGVLRIMGTPGLTIYHVKSHLQKYRLA 465
>gb|BU997001.1|BU997001 HI06J07r HI Hordeum vulgare subsp. vulgare cDNA clone HI06J07
5-PRIME, mRNA sequence
Length = 542
Score = 104 bits (224), Expect = 4e-022
Identities = 41/59 (69%), Positives = 50/59 (84%)
Frame = +1 / +3
Query: 409 ANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
+ K RLRWT +LH RFV+A+ +L GP++ATPKGVL +M V G+TIYHVKSHLQKYRLAK
Sbjct: 243 SGKQRLRWTSDLHSRFVDAIAQLGGPDRATPKGVLTVMGVPGITIYHVKSHLQKYRLAK 419
>gb|BF625895.2|BF625895 HVSMEa0014B15f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0014B15f, mRNA sequence
Length = 486
Score = 102 bits (220), Expect = 2e-021
Identities = 43/56 (76%), Positives = 47/56 (83%)
Frame = +1 / +3
Query: 406 TANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKY 573
T KSR+RWT ELHE+FV+AV KL G EKATPK V K+MKVEGLTIYHVKSHLQ Y
Sbjct: 195 TPTKSRMRWTPELHEKFVDAVDKLGGSEKATPKAVQKVMKVEGLTIYHVKSHLQVY 362
>gb|CA032790.1|CA032790 HX14D10r HX Hordeum vulgare subsp. vulgare cDNA clone HX14D10
5-PRIME, mRNA sequence
Length = 563
Score = 101 bits (218), Expect = 3e-021
Identities = 39/60 (65%), Positives = 52/60 (86%)
Frame = +1 / +1
Query: 406 TANKSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
T K RLRWT+ELH+RFV+AV +L GP+KATPK ++++M V+GLT+YH+KSHLQK+RL K
Sbjct: 232 TDPKPRLRWTVELHDRFVDAVAQLGGPDKATPKTIMRVMGVKGLTLYHLKSHLQKFRLGK 411
>gb|BE558795.1|BE558795 HV_CEb0020G12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0020G12f, mRNA sequence
Length = 823
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +2
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 209 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 379
>gb|BQ758546.1|BQ758546 EBma07_SQ002_G03_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ002_G03 5', mRNA sequence
Length = 678
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +1
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 82 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 252
>gb|BQ758625.1|BQ758625 EBma07_SQ002_G09_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ002_G09 5', mRNA sequence
Length = 686
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +1
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 82 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 252
>gb|BQ758717.1|BQ758717 EBma07_SQ002_I14_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ002_I14 5', mRNA sequence
Length = 592
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +1
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 82 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 252
>gb|BQ760943.1|BQ760943 EBro03_SQ003_O16_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ003_O16 5', mRNA sequence
Length = 540
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +3
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 135 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 305
>gb|CA008548.1|CA008548 HU11E24r HU Hordeum vulgare subsp. vulgare cDNA clone HU11E24
5-PRIME, mRNA sequence
Length = 601
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +1
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 187 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 357
>gb|CA025237.1|CA025237 HZ51O06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ51O06
5-PRIME, mRNA sequence
Length = 578
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +2
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 161 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 331
>gb|CA030194.1|CA030194 HX06F18r HX Hordeum vulgare subsp. vulgare cDNA clone HX06F18
5-PRIME, mRNA sequence
Length = 639
Score = 100 bits (216), Expect = 5e-021
Identities = 39/57 (68%), Positives = 49/57 (85%)
Frame = +1 / +1
Query: 415 KSRLRWTLELHERFVEAVKKLEGPEKATPKGVLKLMKVEGLTIYHVKSHLQKYRLAK 585
K RLRWT +LHERFV+AV +L GPEKATPK +L+ M V+GLT++H+KSHLQKYR+ K
Sbjct: 178 KPRLRWTADLHERFVDAVAQLGGPEKATPKTILRTMGVKGLTLFHLKSHLQKYRMGK 348
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.314 0.132 0.386
Matrix: BLOSUM62
Number of Hits to DB: 246,899,609
Number of Sequences: 312970
Number of extensions: 2925497
Number of successful extensions: 30967
Number of sequences better than 1.0e-020: 26
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 5892498429
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 42 (22.0 bits)