TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067516.2.1
(605 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB880830.1|CB880830 HM07P10w HM Hordeum vulgare subsp. v... 117 4e-041
gb|BJ463876.1|BJ463876 BJ463876 K. Sato unpublished cDNA li... 145 2e-034
gb|BJ468047.1|BJ468047 BJ468047 K. Sato unpublished cDNA li... 74 6e-029
gb|BU971323.1|BU971323 HB17E13r BC Hordeum vulgare subsp. v... 107 2e-027
>gb|CB880830.1|CB880830 HM07P10w HM Hordeum vulgare subsp. vulgare cDNA clone HM07P10
3-PRIME, mRNA sequence
Length = 628
Score = 117 bits (251), Expect(4) = 4e-041
Identities = 49/56 (87%), Positives = 52/56 (92%)
Frame = -2 / -2
Query: 517 KVIYADPRSKLETLKDAFDVSIEAIINKVTKLRRDIIAGQEDKDFIEENSWKYSLL 350
KVIYADPRSKLETLKDAFDVS+ AIINKVT+LRRDII+ EDKDFIEENSWKY LL
Sbjct: 627 KVIYADPRSKLETLKDAFDVSVSAIINKVTQLRRDIISDSEDKDFIEENSWKYELL 460
Score = 100 bits (213), Expect(4) = 1e-040
Identities = 47/56 (83%), Positives = 48/56 (85%)
Frame = +3 / +2
Query: 351 NRLYFQLFSSIKSLSSWPAMISLLNFVTLLMIASMETSNASFNVSSFDLGSAYITF 518
N YFQLFSSIKSLSS MISLLN VTLLMIA ETSNASF+VSSFDLGSAYITF
Sbjct: 461 NSSYFQLFSSIKSLSSLSEMISLLNCVTLLMIAETETSNASFSVSSFDLGSAYITF 628
Score = 84.0 bits (177), Expect(4) = 7e-030
Identities = 37/56 (66%), Positives = 41/56 (73%)
Frame = +2 / +1
Query: 350 *QTILPAILFDKIFVFLACDDISSQLCHFVNDRLYGNIKCIFQCLQFRSWIGIYHL 517
*Q ILPAILF+KIF+F DDISSQLCHFV+D NIK + QFR WI IYHL
Sbjct: 460 *QFILPAILFNKIFIFAVGDDISSQLCHFVDDCRNRNIKRVL*RFQFRPWISIYHL 627
Score = 71.6 bits (150), Expect(4) = 1e-022
Identities = 36/56 (64%), Positives = 41/56 (73%)
Frame = -3 / -3
Query: 516 R*YMPIQDRNWRH*KMHLMFP*RRSLTK*QS*EEISSQAKKTKILSKRIAGSIVC* 349
R*YM IQ RNW+ *+ LMF R+S TK* +*EEISS K KIL KRIAGS+ C*
Sbjct: 626 R*YMLIQGRNWKR*RTRLMFRFRQSSTK*HN*EEISSPTAKIKILLKRIAGSMNC* 459
Score = 43.7 bits (89), Expect(4) = 1e-040
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = +3 / +2
Query: 285 LSLGFEKNGSHL*GPIACCPSSN 353
L LGFEKNGSH *GPI CP+S+
Sbjct: 401 LGLGFEKNGSHS*GPIVRCPNSS 469
Score = 42.8 bits (87), Expect(4) = 4e-041
Identities = 20/33 (60%), Positives = 22/33 (66%)
Frame = -3 / -3
Query: 303 SRSLKIKVEILVVHLQKLPRILGKVNREDRIEP 205
S S K V+ILV+H K PRILGK N E RI P
Sbjct: 413 SPSPKTWVQILVIHQLKQPRILGKSNEETRIRP 315
Score = 41.4 bits (84), Expect(4) = 1e-040
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = +3 / +2
Query: 228 SLFQEFLAASADEPLESPPLSLG 296
S+FQEFLAASADE ES P+SLG
Sbjct: 338 SIFQEFLAASADELPESAPMSLG 406
Score = 38.2 bits (77), Expect(4) = 7e-030
Identities = 19/31 (61%), Positives = 20/31 (64%)
Frame = +2 / +1
Query: 206 GSILSSLFTFPRILGSFCR*TTRISTFIFRL 298
G IL S F FPRILG F * TRI T +F L
Sbjct: 316 GLILVSSFDFPRILGCFS**ITRICTHVFGL 408
Score = 36.8 bits (74), Expect(4) = 4e-041
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -2
Query: 343 GQQAIGPHKWDPFF 302
GQ+ IGPH+WDPFF
Sbjct: 459 GQRTIGPHEWDPFF 418
Score = 36.8 bits (74), Expect(4) = 1e-040
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +2 / +2
Query: 35 FGGMASYNCRVT*IINDVHDHHNYWI 112
FGG SY C II D+HDH +Y I
Sbjct: 143 FGGTTSYTCCRDWIIKDIHDHQDYSI 220
Score = 33.1 bits (66), Expect(4) = 7e-030
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2 / +1
Query: 296 LREEWIPFVRSNCLLS 343
LREEWIP +RS+C LS
Sbjct: 412 LREEWIPLMRSDCPLS 459
Score = 32.2 bits (64), Expect(4) = 1e-022
Identities = 14/24 (58%), Positives = 18/24 (75%)
Frame = -2 / -2
Query: 259 AEAAKNSWKSEQRGQD*TMSWSSL 188
AEAAKNSWK E+ Q+*T ++L
Sbjct: 369 AEAAKNSWKIERGDQN*TAQLAAL 298
Score = 30.9 bits (61), Expect(4) = 1e-022
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = -3 / -3
Query: 342 DSRQLDLTNGIHSSRSLKIKVEILVVHLQKL 250
DS Q DL +GIHSSRS K + ++ + +L
Sbjct: 458 DSGQSDLMSGIHSSRSPSPKTWVQILVIHQL 366
Score = 29.9 bits (59), Expect(4) = 7e-030
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1 / +1
Query: 28 KAFWWNGFIQLQGDIDHQ*CS*SP 99
K FWW F+ L +DHQ +*SP
Sbjct: 136 KDFWWYHFLHLL*GLDHQGHT*SP 207
Score = 26.3 bits (51), Expect(4) = 4e-041
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -3 / -2
Query: 99 W*S*TSLMIYVTLQLYEAIPPK 34
W*S SLMI Q+ E +PPK
Sbjct: 207 W*SCMSLMIQSLQQV*EVVPPK 142
Score = 25.8 bits (50), Expect(4) = 1e-022
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = -2 / -1
Query: 112 DPVVMVIMNIIDDLCHPAVV*SHSTK 35
D VV+VIM ++DD A V S +TK
Sbjct: 220 DAVVLVIMYVLDDPVPTAGVRSGTTK 143
>gb|BJ463876.1|BJ463876 BJ463876 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30n18 5', mRNA sequence
Length = 680
Score = 145 bits (312), Expect = 2e-034
Identities = 62/72 (86%), Positives = 67/72 (93%)
Frame = -2 / +3
Query: 604 GNASIEERLKSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVSIEAIINKVTK 425
GN S+EE L+SIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVS+ AIINKVT+
Sbjct: 465 GNVSVEEILRSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVSVSAIINKVTQ 644
Query: 424 LRRDIIAGQEDK 389
LRRDII+ EDK
Sbjct: 645 LRRDIISDSEDK 680
Score = 124 bits (266), Expect = 3e-028
Identities = 58/72 (80%), Positives = 62/72 (86%)
Frame = +3 / -2
Query: 390 LSSWPAMISLLNFVTLLMIASMETSNASFNVSSFDLGSAYITFGIRLMTSSLICLTTSG* 569
LSS MISLLN VTLLMIA ETSNASF+VSSFDLGSAYITFG+RL+TSSLICLTTSG
Sbjct: 679 LSSLSEMISLLNCVTLLMIAETETSNASFSVSSFDLGSAYITFGMRLITSSLICLTTSGW 500
Query: 570 ILFSLSSMLAFP 605
IL S+SS L FP
Sbjct: 499 ILLSISSTLTFP 464
>gb|BJ468047.1|BJ468047 BJ468047 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30n18 3', mRNA sequence
Length = 573
Score = 73.9 bits (155), Expect(4) = 3e-028
Identities = 30/37 (81%), Positives = 33/37 (89%)
Frame = -2 / -3
Query: 460 VSIEAIINKVTKLRRDIIAGQEDKDFIEENSWKYSLL 350
VS+ AIINKVT+LRRDII+ EDKDFIEENSWKY LL
Sbjct: 571 VSVSAIINKVTQLRRDIISDSEDKDFIEENSWKYELL 461
Score = 60.2 bits (125), Expect(4) = 6e-029
Identities = 25/32 (78%), Positives = 28/32 (87%)
Frame = +2 / +2
Query: 350 *QTILPAILFDKIFVFLACDDISSQLCHFVND 445
*Q ILPAILF+KIF+F DDISSQLCHFV+D
Sbjct: 461 *QFILPAILFNKIFIFAVGDDISSQLCHFVDD 556
Score = 59.7 bits (124), Expect(4) = 8e-023
Identities = 29/37 (78%), Positives = 29/37 (78%)
Frame = +3 / +3
Query: 351 NRLYFQLFSSIKSLSSWPAMISLLNFVTLLMIASMET 461
N YFQLFSSIKSLSS MISLLN VTLLMIA ET
Sbjct: 462 NSSYFQLFSSIKSLSSLSEMISLLNCVTLLMIAETET 572
Score = 43.7 bits (89), Expect(4) = 6e-029
Identities = 17/23 (73%), Positives = 19/23 (82%)
Frame = +3 / +3
Query: 285 LSLGFEKNGSHL*GPIACCPSSN 353
L LGFEKNGSH *GPI CP+S+
Sbjct: 402 LGLGFEKNGSHS*GPIVRCPNSS 470
Score = 42.8 bits (87), Expect(4) = 3e-028
Identities = 20/33 (60%), Positives = 22/33 (66%)
Frame = -3 / -1
Query: 303 SRSLKIKVEILVVHLQKLPRILGKVNREDRIEP 205
S S K V+ILV+H K PRILGK N E RI P
Sbjct: 414 SPSPKTWVQILVIHQLKQPRILGKSNEETRIRP 316
Score = 41.4 bits (84), Expect(4) = 6e-029
Identities = 18/23 (78%), Positives = 20/23 (86%)
Frame = +3 / +3
Query: 228 SLFQEFLAASADEPLESPPLSLG 296
S+FQEFLAASADE ES P+SLG
Sbjct: 339 SIFQEFLAASADELPESAPMSLG 407
Score = 38.2 bits (77), Expect(4) = 8e-023
Identities = 19/31 (61%), Positives = 20/31 (64%)
Frame = +2 / +2
Query: 206 GSILSSLFTFPRILGSFCR*TTRISTFIFRL 298
G IL S F FPRILG F * TRI T +F L
Sbjct: 317 GLILVSSFDFPRILGCFS**ITRICTHVFGL 409
Score = 36.8 bits (74), Expect(4) = 6e-029
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +2 / +3
Query: 35 FGGMASYNCRVT*IINDVHDHHNYWI 112
FGG SY C II D+HDH +Y I
Sbjct: 144 FGGTTSYTCCRDWIIKDIHDHQDYSI 221
Score = 36.8 bits (74), Expect(4) = 3e-028
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -2 / -3
Query: 343 GQQAIGPHKWDPFF 302
GQ+ IGPH+WDPFF
Sbjct: 460 GQRTIGPHEWDPFF 419
Score = 33.1 bits (66), Expect(4) = 8e-023
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2 / +2
Query: 296 LREEWIPFVRSNCLLS 343
LREEWIP +RS+C LS
Sbjct: 413 LREEWIPLMRSDCPLS 460
Score = 29.9 bits (59), Expect(4) = 8e-023
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = +1 / +2
Query: 28 KAFWWNGFIQLQGDIDHQ*CS*SP 99
K FWW F+ L +DHQ +*SP
Sbjct: 137 KDFWWYHFLHLL*GLDHQGHT*SP 208
Score = 26.3 bits (51), Expect(4) = 3e-028
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = -3 / -3
Query: 99 W*S*TSLMIYVTLQLYEAIPPK 34
W*S SLMI Q+ E +PPK
Sbjct: 208 W*SCMSLMIQSLQQV*EVVPPK 143
>gb|BU971323.1|BU971323 HB17E13r BC Hordeum vulgare subsp. vulgare cDNA clone HB17E13
5-PRIME, mRNA sequence
Length = 519
Score = 107 bits (228), Expect(2) = 2e-027
Identities = 45/49 (91%), Positives = 47/49 (95%)
Frame = -2 / +2
Query: 604 GNASIEERLKSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDV 458
GN S+EE L+SIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDV
Sbjct: 311 GNVSVEEILRSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDV 457
Score = 94.5 bits (200), Expect(2) = 2e-023
Identities = 41/50 (82%), Positives = 46/50 (92%)
Frame = +3 / -1
Query: 456 ETSNASFNVSSFDLGSAYITFGIRLMTSSLICLTTSG*ILFSLSSMLAFP 605
+TSNASF+VSSFDLGSAYITFG+RL+TSSLICLTTSG IL S+SS L FP
Sbjct: 459 KTSNASFSVSSFDLGSAYITFGMRLITSSLICLTTSGWILLSISSTLTFP 310
Score = 34.5 bits (69), Expect(2) = 2e-027
Identities = 14/18 (77%), Positives = 17/18 (94%)
Frame = -2 / +3
Query: 457 SIEAIINKVTKLRRDIIA 404
S+ AIINKVT+LRRDII+
Sbjct: 459 SVSAIINKVTQLRRDIIS 512
Score = 33.6 bits (67), Expect(2) = 2e-023
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = +2 / -2
Query: 407 DDISSQLCHFVND 445
DDISSQLCHFV+D
Sbjct: 509 DDISSQLCHFVDD 471
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 248,609,079
Number of Sequences: 312970
Number of extensions: 3092750
Number of successful extensions: 26094
Number of sequences better than 1.0e-020: 5
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6362506350
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)