TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3064721.2.1
(689 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. v... 119 1e-059
gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA li... 119 1e-059
gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-a... 119 1e-046
gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA li... 107 1e-046
gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. v... 107 4e-045
gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA li... 79 3e-044
gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgar... 102 2e-043
gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm librar... 102 2e-043
gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA li... 119 2e-043
gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-a... 102 2e-043
gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA li... 109 4e-041
gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA li... 109 4e-041
gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA li... 79 5e-039
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 102 2e-038
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 110 1e-037
gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa... 97 2e-036
gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-a... 119 3e-035
gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green... 97 2e-034
gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA li... 69 2e-033
gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA li... 65 3e-032
gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA li... 70 4e-031
gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA li... 70 4e-031
gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA li... 70 4e-031
gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA li... 70 4e-031
gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no... 65 3e-028
gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA li... 61 1e-025
gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA li... 75 2e-024
gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. v... 70 4e-023
gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA li... 106 1e-022
>gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. vulgare cDNA clone HX12J15
5-PRIME, mRNA sequence
Length = 653
Score = 119 bits (255), Expect(3) = 1e-059
Identities = 42/53 (79%), Positives = 49/53 (92%)
Frame = +2 / -3
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNR 163
ACGWA+GMN+FDL+ WR+QNITE+YH WQKLNE+ LLWKLGTLP GLVTFWN+
Sbjct: 534 ACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLGTLPPGLVTFWNK 376
Score = 88.6 bits (187), Expect(3) = 5e-032
Identities = 36/57 (63%), Positives = 42/57 (73%)
Frame = -3 / +2
Query: 174 RERMRFQKVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLHPDKSNMFMPYAQPQA 4
R + FQ VTRP GRVPSFH+S S +FCQ W*+SVMFC LH +SN F+P AQP A
Sbjct: 365 RGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKAQPHA 535
Score = 78.5 bits (165), Expect(3) = 1e-059
Identities = 28/37 (75%), Positives = 33/37 (89%)
Frame = +1 / -3
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
TFPLDRSWH LGLGYNP+V+ +D+ RA VIH+NGNLK
Sbjct: 375 TFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLK 265
Score = 70.7 bits (148), Expect(3) = 1e-059
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +2 / -3
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP*TANS 388
WLEIGLPK+R YWS +++YDQ FL+ECNINP*+ S
Sbjct: 261 WLEIGLPKFRSYWSKYLDYDQPFLRECNINP*SKTS 154
Score = 49.2 bits (101), Expect(3) = 5e-032
Identities = 22/40 (55%), Positives = 26/40 (65%)
Frame = -1 / +2
Query: 284 ARSFKFPLKWMTVARLISFSLTFGLYPSPS*CHERSRGNV 165
++ F+ PL MT AR S LT GL P P CH+RSRGNV
Sbjct: 257 SQGFRLPL*CMTAARSRSLLLTCGL*PRPRTCHDRSRGNV 376
Score = 37.7 bits (76), Expect(3) = 5e-032
Identities = 20/36 (55%), Positives = 22/36 (61%)
Frame = -3 / +2
Query: 387 LFAVHGFILHSCRNTWS*LTCADQYLRYLGNPISSQ 280
L +HG +LHS R S* DQ LR LG PISSQ
Sbjct: 155 LVLLHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQ 262
>gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 3', mRNA sequence
Length = 643
Score = 119 bits (255), Expect(3) = 1e-059
Identities = 42/53 (79%), Positives = 49/53 (92%)
Frame = +2 / -3
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNR 163
ACGWA+GMN+FDL+ WR+QNITE+YH WQKLNE+ LLWKLGTLP GLVTFWN+
Sbjct: 521 ACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLGTLPPGLVTFWNK 363
Score = 88.6 bits (187), Expect(3) = 5e-032
Identities = 36/57 (63%), Positives = 42/57 (73%)
Frame = -3 / +1
Query: 174 RERMRFQKVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLHPDKSNMFMPYAQPQA 4
R + FQ VTRP GRVPSFH+S S +FCQ W*+SVMFC LH +SN F+P AQP A
Sbjct: 352 RGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKAQPHA 522
Score = 78.5 bits (165), Expect(3) = 1e-059
Identities = 28/37 (75%), Positives = 33/37 (89%)
Frame = +1 / -3
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
TFPLDRSWH LGLGYNP+V+ +D+ RA VIH+NGNLK
Sbjct: 362 TFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLK 252
Score = 70.7 bits (148), Expect(3) = 1e-059
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +2 / -3
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP*TANS 388
WLEIGLPK+R YWS +++YDQ FL+ECNINP*+ S
Sbjct: 248 WLEIGLPKFRSYWSKYLDYDQPFLRECNINP*SKTS 141
Score = 49.2 bits (101), Expect(3) = 5e-032
Identities = 22/40 (55%), Positives = 26/40 (65%)
Frame = -1 / +1
Query: 284 ARSFKFPLKWMTVARLISFSLTFGLYPSPS*CHERSRGNV 165
++ F+ PL MT AR S LT GL P P CH+RSRGNV
Sbjct: 244 SQGFRLPL*CMTAARSRSLLLTCGL*PRPRTCHDRSRGNV 363
Score = 37.7 bits (76), Expect(3) = 5e-032
Identities = 20/36 (55%), Positives = 22/36 (61%)
Frame = -3 / +1
Query: 387 LFAVHGFILHSCRNTWS*LTCADQYLRYLGNPISSQ 280
L +HG +LHS R S* DQ LR LG PISSQ
Sbjct: 142 LVLLHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQ 249
>gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006H13f, mRNA sequence
Length = 1018
Score = 119 bits (255), Expect(3) = 1e-046
Identities = 42/53 (79%), Positives = 49/53 (92%)
Frame = +2 / +1
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNR 163
ACGWA+GMN+FDL+ WR+QNITE+YH WQKLNE+ LLWKLGTLP GLVTFWN+
Sbjct: 370 ACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLGTLPPGLVTFWNK 528
Score = 87.7 bits (185), Expect(2) = 4e-024
Identities = 35/52 (67%), Positives = 40/52 (76%)
Frame = -3 / -3
Query: 159 FQKVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLHPDKSNMFMPYAQPQA 4
FQ VTRP GRVPSFH+S S +FCQ W*+SVMFC LH +SN F+P AQP A
Sbjct: 524 FQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKAQPHA 369
Score = 73.0 bits (153), Expect(3) = 1e-046
Identities = 26/37 (70%), Positives = 32/37 (86%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
TFPLDRSWH LGLGYNP+V+ +D+ RA VIH+ G+LK
Sbjct: 529 TFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYXGHLK 639
Score = 43.2 bits (88), Expect(2) = 4e-024
Identities = 20/40 (50%), Positives = 24/40 (60%)
Frame = -1 / -3
Query: 284 ARSFKFPLKWMTVARLISFSLTFGLYPSPS*CHERSRGNV 165
++ F+ P MT AR S LT GL P P CH+RS GNV
Sbjct: 647 SQGFRCPX*CMTAARSRSLLLTCGL*PRPRTCHDRSXGNV 528
Score = 32.2 bits (64), Expect(3) = 1e-046
Identities = 9/17 (52%), Positives = 14/17 (82%)
Frame = +2 / +3
Query: 299 PKYRKYWSAHVNYDQVF 349
P++R YWS +++YDQ F
Sbjct: 660 PQFRSYWSKYLDYDQXF 710
>gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 3', mRNA sequence
Length = 625
Score = 107 bits (229), Expect(3) = 1e-046
Identities = 38/58 (65%), Positives = 46/58 (79%)
Frame = +2 / -3
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
+ACGWAYGMN FDL+ WR++ TE YH WQ NENRLLWKLGTLP GL+TF++ + L
Sbjct: 605 NACGWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLGTLPPGLITFYSTTKPL 432
Score = 70.3 bits (147), Expect(3) = 1e-046
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / -3
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 443 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 333
Score = 46.9 bits (96), Expect(3) = 1e-046
Identities = 13/31 (41%), Positives = 24/31 (77%)
Frame = +2 / -3
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP 373
WL+IG+ ++R+ W+ +V+YD F+++CN P
Sbjct: 329 WLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 237
>gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. vulgare cDNA clone HX14D07
5-PRIME, mRNA sequence
Length = 529
Score = 107 bits (229), Expect(3) = 4e-045
Identities = 38/58 (65%), Positives = 46/58 (79%)
Frame = +2 / +2
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
+ACGWAYGMN FDL+ WR++ TE YH WQ NENRLLWKLGTLP GL+TF++ + L
Sbjct: 173 NACGWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLGTLPPGLITFYSTTKPL 346
Score = 70.3 bits (147), Expect(3) = 4e-045
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / +2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 335 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 445
Score = 41.8 bits (85), Expect(3) = 4e-045
Identities = 11/27 (40%), Positives = 22/27 (81%)
Frame = +2 / +2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQEC 361
WL+IG+ ++R+ W+ +V+YD F+++C
Sbjct: 449 WLDIGMNQFRQLWTKYVDYDDSFIRQC 529
>gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19o15 3', mRNA sequence
Length = 635
Score = 78.5 bits (165), Expect(3) = 3e-044
Identities = 28/37 (75%), Positives = 33/37 (89%)
Frame = +1 / -2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
TFPLDRSWH LGLGYNP+V+ +D+ RA VIH+NGNLK
Sbjct: 547 TFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLK 437
Score = 70.7 bits (148), Expect(3) = 3e-044
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +2 / -2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP*TANS 388
WLEIGLPK+R YWS +++YDQ FL+ECNINP*+ S
Sbjct: 433 WLEIGLPKFRSYWSKYLDYDQPFLRECNINP*SKTS 326
Score = 67.5 bits (141), Expect(3) = 3e-044
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = +2 / -2
Query: 77 YHTWQKLNENRLLWKLGTLPAGLVTFWNR 163
YH WQKLNE+ LLWKLGTLP GLVTFWN+
Sbjct: 634 YHFWQKLNEDGLLWKLGTLPPGLVTFWNK 548
Score = 52.8 bits (109), Expect(4) = 2e-022
Identities = 21/33 (63%), Positives = 24/33 (72%)
Frame = -3 / +3
Query: 174 RERMRFQKVTRPAGRVPSFHNSLFSFNFCQVW* 76
R + FQ VTRP GRVPSFH+S S +FCQ W*
Sbjct: 537 RGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW* 635
Score = 49.2 bits (101), Expect(4) = 2e-022
Identities = 22/40 (55%), Positives = 26/40 (65%)
Frame = -1 / +3
Query: 284 ARSFKFPLKWMTVARLISFSLTFGLYPSPS*CHERSRGNV 165
++ F+ PL MT AR S LT GL P P CH+RSRGNV
Sbjct: 429 SQGFRLPL*CMTAARSRSLLLTCGL*PRPRTCHDRSRGNV 548
Score = 37.7 bits (76), Expect(4) = 2e-022
Identities = 20/36 (55%), Positives = 22/36 (61%)
Frame = -3 / +3
Query: 387 LFAVHGFILHSCRNTWS*LTCADQYLRYLGNPISSQ 280
L +HG +LHS R S* DQ LR LG PISSQ
Sbjct: 327 LVLLHGLMLHSRRKG*S*SRYFDQ*LRNLGRPISSQ 434
Score = 22.6 bits (43), Expect(4) = 2e-022
Identities = 10/37 (27%), Positives = 17/37 (45%)
Frame = -2 / +1
Query: 475 HPRQLKLPRAQRKLV*GIVKEKTVN*AYTTICCSRVY 365
HP L L R + I+ + +YTT+ C + +
Sbjct: 64 HPGSLGLSRVFLTSMLQILNGSNLTFSYTTMACQKAF 174
>gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010D065 5-PRIME, mRNA sequence
Length = 786
Score = 102 bits (218), Expect(3) = 2e-043
Identities = 34/52 (65%), Positives = 45/52 (86%)
Frame = +2 / +1
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
+ACGWAYGMN+FDL W+ ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 154 NACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFY 309
Score = 65.2 bits (136), Expect(3) = 2e-043
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 316 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 426
Score = 46.4 bits (95), Expect(3) = 2e-043
Identities = 12/30 (40%), Positives = 22/30 (73%)
Frame = +2 / +1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNIN 370
WLE+ + KYR YW+ ++ YD +++ CN++
Sbjct: 430 WLELAMTKYRPYWTRYIKYDHPYIRGCNLS 519
>gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B7 5' similar to 68 kDa
protein, mRNA sequence
Length = 616
Score = 102 bits (218), Expect(3) = 2e-043
Identities = 34/52 (65%), Positives = 45/52 (86%)
Frame = +2 / +1
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
+ACGWAYGMN+FDL W+ ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 115 NACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFY 270
Score = 65.2 bits (136), Expect(3) = 2e-043
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 277 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 387
Score = 46.4 bits (95), Expect(3) = 2e-043
Identities = 12/30 (40%), Positives = 22/30 (73%)
Frame = +2 / +1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNIN 370
WLE+ + KYR YW+ ++ YD +++ CN++
Sbjct: 391 WLELAMTKYRPYWTRYIKYDHPYIRGCNLS 480
>gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 5', mRNA sequence
Length = 612
Score = 119 bits (255), Expect(2) = 2e-043
Identities = 42/53 (79%), Positives = 49/53 (92%)
Frame = +2 / +2
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNR 163
ACGWA+GMN+FDL+ WR+QNITE+YH WQKLNE+ LLWKLGTLP GLVTFWN+
Sbjct: 203 ACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLGTLPPGLVTFWNK 361
Score = 88.6 bits (187), Expect(3) = 6e-029
Identities = 36/57 (63%), Positives = 42/57 (73%)
Frame = -3 / -1
Query: 174 RERMRFQKVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLHPDKSNMFMPYAQPQA 4
R + FQ VTRP GRVPSFH+S S +FCQ W*+SVMFC LH +SN F+P AQP A
Sbjct: 372 RGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKAQPHA 202
Score = 76.2 bits (160), Expect(2) = 2e-043
Identities = 33/70 (47%), Positives = 45/70 (64%)
Frame = +1 / +2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLKLLA*DWIAQIPQVLVSTRQLRPS 345
TFPLDRSWH LGLGYNP+V+ +D+ RA + LA*DW Q+P++LV L +
Sbjct: 362 TFPLDRSWHVLGLGYNPHVSSRDLERARSHTLQWQPEALA*DWPPQVPKLLVKVP*L*SA 541
Query: 346 VSTRVQYKPV 375
S +Q++ V
Sbjct: 542 FSAGMQHQSV 571
Score = 38.6 bits (78), Expect(3) = 6e-029
Identities = 15/36 (41%), Positives = 24/36 (66%)
Frame = -2 / -2
Query: 385 ICCSRVYIALL*KHLVVIDVC*PVLAVFGQSNLKPG 278
+ SR+ +A K L+++ V *PV + G++NLKPG
Sbjct: 581 VLASRIDVAFPQKRLIIVKVL*PVASELGEANLKPG 474
Score = 37.7 bits (76), Expect(3) = 6e-029
Identities = 17/27 (62%), Positives = 18/27 (66%)
Frame = -1 / -1
Query: 245 ARLISFSLTFGLYPSPS*CHERSRGNV 165
AR S LT GL P P CH+RSRGNV
Sbjct: 441 ARSRSLLLTCGL*PRPRTCHDRSRGNV 361
>gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018F09f, mRNA sequence
Length = 856
Score = 102 bits (217), Expect(3) = 2e-043
Identities = 34/51 (66%), Positives = 44/51 (86%)
Frame = +2 / +1
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
ACGWAYGMN+FDL W+ ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 1 ACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFY 153
Score = 65.2 bits (136), Expect(3) = 2e-043
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 160 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 270
Score = 46.4 bits (95), Expect(3) = 2e-043
Identities = 12/30 (40%), Positives = 22/30 (73%)
Frame = +2 / +1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNIN 370
WLE+ + KYR YW+ ++ YD +++ CN++
Sbjct: 274 WLELAMTKYRPYWTRYIKYDHPYIRGCNLS 363
>gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 5', mRNA sequence
Length = 630
Score = 109 bits (233), Expect(3) = 4e-041
Identities = 38/52 (73%), Positives = 45/52 (86%)
Frame = +2 / +2
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWN 160
ACGWA+GMNMFDL W+K+NIT +YH WQ LNE+R LWKLGTLP GL+TF+N
Sbjct: 128 ACGWAFGMNMFDLKEWKKRNITGIYHYWQNLNEDRKLWKLGTLPPGLITFYN 283
Score = 59.3 bits (123), Expect(3) = 4e-041
Identities = 21/37 (56%), Positives = 27/37 (72%)
Frame = +1 / +2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD +WH LGLGY+P V+ +I A V+H+NGN K
Sbjct: 287 THPLDHTWHVLGLGYDPAVDIAEIENAAVVHYNGNYK 397
Score = 37.3 bits (75), Expect(3) = 4e-041
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +2 / +2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQEC 361
WL++ + KY+ YWS +V+ D ++ C
Sbjct: 401 WLDLAISKYKAYWSKYVDVDSSHIRHC 481
>gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 3', mRNA sequence
Length = 565
Score = 109 bits (233), Expect(3) = 4e-041
Identities = 38/52 (73%), Positives = 45/52 (86%)
Frame = +2 / -1
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWN 160
ACGWA+GMNMFDL W+K+NIT +YH WQ LNE+R LWKLGTLP GL+TF+N
Sbjct: 442 ACGWAFGMNMFDLKEWKKRNITGIYHYWQNLNEDRKLWKLGTLPPGLITFYN 287
Score = 59.3 bits (123), Expect(3) = 4e-041
Identities = 21/37 (56%), Positives = 27/37 (72%)
Frame = +1 / -1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD +WH LGLGY+P V+ +I A V+H+NGN K
Sbjct: 283 THPLDHTWHVLGLGYDPAVDIAEIENAAVVHYNGNYK 173
Score = 37.3 bits (75), Expect(3) = 4e-041
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +2 / -1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQEC 361
WL++ + KY+ YWS +V+ D ++ C
Sbjct: 169 WLDLAISKYKAYWSKYVDVDSSHIRHC 89
>gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25o11 3', mRNA sequence
Length = 495
Score = 78.5 bits (165), Expect(3) = 5e-039
Identities = 28/37 (75%), Positives = 33/37 (89%)
Frame = +1 / -1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
TFPLDRSWH LGLGYNP+V+ +D+ RA VIH+NGNLK
Sbjct: 429 TFPLDRSWHVLGLGYNPHVSSRDLERAAVIHYNGNLK 319
Score = 70.7 bits (148), Expect(3) = 5e-039
Identities = 25/36 (69%), Positives = 31/36 (86%)
Frame = +2 / -1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP*TANS 388
WLEIGLPK+R YWS +++YDQ FL+ECNINP*+ S
Sbjct: 315 WLEIGLPKFRSYWSKYLDYDQPFLRECNINP*SKTS 208
Score = 50.1 bits (103), Expect(3) = 5e-039
Identities = 18/22 (81%), Positives = 20/22 (90%)
Frame = +2 / -1
Query: 98 NENRLLWKLGTLPAGLVTFWNR 163
NE+ LLWKLGTLP GLVTFWN+
Sbjct: 495 NEDGLLWKLGTLPPGLVTFWNK 430
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 102 bits (218), Expect(3) = 2e-038
Identities = 34/52 (65%), Positives = 45/52 (86%)
Frame = +2 / +2
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
+ACGWAYGMN+FDL W+ ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 281 NACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFY 436
Score = 65.2 bits (136), Expect(3) = 2e-038
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / +2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 443 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 553
Score = 29.5 bits (58), Expect(3) = 2e-038
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2 / +2
Query: 281 WLEIGLPKYRKYWS 322
WLE+ + KYR YW+
Sbjct: 557 WLELAMTKYRPYWT 598
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 110 bits (235), Expect(2) = 1e-037
Identities = 38/52 (73%), Positives = 46/52 (88%)
Frame = +2 / +3
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
+ACGWAYGMNMFDL W+K++IT +YH WQ +NENRLLWKLGTLP GL+TF+
Sbjct: 405 NACGWAYGMNMFDLEEWKKKDITGIYHKWQNMNENRLLWKLGTLPPGLMTFY 560
Score = 78.0 bits (164), Expect(2) = 3e-024
Identities = 31/50 (62%), Positives = 36/50 (72%)
Frame = -3 / -3
Query: 153 KVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLHPDKSNMFMPYAQPQA 4
KV RP G VPSFH+SLFSF FC +W* VM F H +SN+F+P AQP A
Sbjct: 556 KVMRPGGSVPSFHSSLFSFMFCHLW*IPVMSFFFHSSRSNIFIP*AQPHA 407
Score = 66.1 bits (138), Expect(2) = 1e-037
Identities = 24/37 (64%), Positives = 28/37 (75%)
Frame = +1 / +3
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP V +I A VIH+NGN+K
Sbjct: 567 THPLDKSWHVLGLGYNPTVEHAEIDTAAVIHYNGNMK 677
Score = 53.3 bits (110), Expect(2) = 3e-024
Identities = 23/37 (62%), Positives = 25/37 (67%)
Frame = -1 / -3
Query: 275 FKFPLKWMTVARLISFSLTFGLYPSPS*CHERSRGNV 165
F FPL WMT A IS T GLYP+PS CH+ SRG V
Sbjct: 676 FMFPL*WMTAAVSISACSTVGLYPNPSTCHDLSRGCV 566
>gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016D06f, mRNA sequence
Length = 490
Score = 96.8 bits (205), Expect(3) = 2e-036
Identities = 33/58 (56%), Positives = 44/58 (75%)
Frame = +2 / +3
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
+ CGWA+GMN+FDL WRK N T +YH WQ+ N ++LLW+ GTLPAGL+TF+ + L
Sbjct: 33 YTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNADQLLWRTGTLPAGLLTFYGLMEPL 206
Score = 57.4 bits (119), Expect(3) = 2e-036
Identities = 19/35 (54%), Positives = 28/35 (80%)
Frame = +1 / +3
Query: 172 PLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
PLDR WH LGLGY+ +++++ I A V+H+NGN+K
Sbjct: 201 PLDRRWHLLGLGYDVDIDDRLIESAAVVHYNGNMK 305
Score = 36.3 bits (73), Expect(3) = 2e-036
Identities = 9/27 (33%), Positives = 17/27 (62%)
Frame = +2 / +3
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQEC 361
WL++ + +Y+ W HVN+ ++EC
Sbjct: 309 WLKLAIHRYKSIWERHVNFSHPHIREC 389
>gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012C17f, mRNA sequence
Length = 785
Score = 119 bits (255), Expect(2) = 3e-035
Identities = 42/53 (79%), Positives = 49/53 (92%)
Frame = +2 / +1
Query: 5 ACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNR 163
ACGWA+GMN+FDL+ WR+QNITE+YH WQKLNE+ LLWKLGTLP GLVTFWN+
Sbjct: 370 ACGWAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLGTLPPGLVTFWNK 528
Score = 88.6 bits (187), Expect(2) = 6e-022
Identities = 36/57 (63%), Positives = 42/57 (73%)
Frame = -3 / -1
Query: 174 RERMRFQKVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLHPDKSNMFMPYAQPQA 4
R + FQ VTRP GRVPSFH+S S +FCQ W*+SVMFC LH +SN F+P AQP A
Sbjct: 539 RGNVLFQNVTRPGGRVPSFHSSPSSLSFCQKW*SSVMFCRLHSARSNTFIPKAQPHA 369
Score = 48.7 bits (100), Expect(2) = 3e-035
Identities = 17/20 (85%), Positives = 19/20 (95%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVN 225
TFPLDRSWH LGLGYNP+V+
Sbjct: 529 TFPLDRSWHVLGLGYNPHVS 588
Score = 35.0 bits (70), Expect(2) = 6e-022
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = -1 / -1
Query: 224 LTFGLYPSPS*CHERSRGNV 165
LT GL P P CH+RSRGNV
Sbjct: 587 LTCGL*PRPRTCHDRSRGNV 528
>gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0025O15f, mRNA sequence
Length = 674
Score = 96.8 bits (205), Expect(3) = 2e-034
Identities = 33/58 (56%), Positives = 44/58 (75%)
Frame = +2 / +1
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
+ CGWA+GMN+FDL WRK N T +YH WQ+ N ++LLW+ GTLPAGL+TF+ + L
Sbjct: 211 YTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNADQLLWRTGTLPAGLLTFYGLMEPL 384
Score = 57.4 bits (119), Expect(3) = 2e-034
Identities = 19/35 (54%), Positives = 28/35 (80%)
Frame = +1 / +1
Query: 172 PLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
PLDR WH LGLGY+ +++++ I A V+H+NGN+K
Sbjct: 379 PLDRRWHLLGLGYDVDIDDRLIESAAVVHYNGNMK 483
Score = 29.0 bits (57), Expect(3) = 2e-034
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = +2 / +1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQE 358
WL++ + Y+ W A VN+ ++E
Sbjct: 487 WLKLAIHPYKSIWGADVNFSHPHIRE 564
>gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd23f10, mRNA
sequence
Length = 643
Score = 68.9 bits (144), Expect(3) = 2e-033
Identities = 22/36 (61%), Positives = 31/36 (86%)
Frame = +2 / -2
Query: 50 WRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
W+ ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 636 WKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFY 529
Score = 65.2 bits (136), Expect(3) = 2e-033
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / -2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 522 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 412
Score = 46.4 bits (95), Expect(3) = 2e-033
Identities = 12/30 (40%), Positives = 22/30 (73%)
Frame = +2 / -2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNIN 370
WLE+ + KYR YW+ ++ YD +++ CN++
Sbjct: 408 WLELAMTKYRPYWTRYIKYDHPYIRGCNLS 319
>gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 3', mRNA sequence
Length = 634
Score = 65.2 bits (136), Expect(3) = 3e-032
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / -1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 520 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 410
Score = 64.8 bits (135), Expect(3) = 3e-032
Identities = 22/35 (62%), Positives = 30/35 (85%)
Frame = +2 / -1
Query: 53 RKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
R ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 631 RTKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFY 527
Score = 46.4 bits (95), Expect(3) = 3e-032
Identities = 12/30 (40%), Positives = 22/30 (73%)
Frame = +2 / -1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNIN 370
WLE+ + KYR YW+ ++ YD +++ CN++
Sbjct: 406 WLELAMTKYRPYWTRYIKYDHPYIRGCNLS 317
>gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 5', mRNA sequence
Length = 589
Score = 70.3 bits (147), Expect(3) = 4e-031
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / +2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 89 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 199
Score = 55.1 bits (114), Expect(3) = 4e-031
Identities = 20/30 (66%), Positives = 24/30 (80%)
Frame = +2 / +2
Query: 86 WQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
WQ NENRLLWKLGTLP GL+TF++ + L
Sbjct: 11 WQTQNENRLLWKLGTLPPGLITFYSTTKPL 100
Score = 46.9 bits (96), Expect(3) = 4e-031
Identities = 13/31 (41%), Positives = 24/31 (77%)
Frame = +2 / +2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP 373
WL+IG+ ++R+ W+ +V+YD F+++CN P
Sbjct: 203 WLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 295
>gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 5', mRNA sequence
Length = 586
Score = 70.3 bits (147), Expect(3) = 4e-031
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / +2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 86 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 196
Score = 55.1 bits (114), Expect(3) = 4e-031
Identities = 20/30 (66%), Positives = 24/30 (80%)
Frame = +2 / +2
Query: 86 WQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
WQ NENRLLWKLGTLP GL+TF++ + L
Sbjct: 8 WQTQNENRLLWKLGTLPPGLITFYSTTKPL 97
Score = 46.9 bits (96), Expect(3) = 4e-031
Identities = 13/31 (41%), Positives = 24/31 (77%)
Frame = +2 / +2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP 373
WL+IG+ ++R+ W+ +V+YD F+++CN P
Sbjct: 200 WLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 292
>gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 3', mRNA sequence
Length = 527
Score = 70.3 bits (147), Expect(3) = 4e-031
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / -2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 442 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 332
Score = 55.1 bits (114), Expect(3) = 4e-031
Identities = 20/30 (66%), Positives = 24/30 (80%)
Frame = +2 / -2
Query: 86 WQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
WQ NENRLLWKLGTLP GL+TF++ + L
Sbjct: 520 WQTQNENRLLWKLGTLPPGLITFYSTTKPL 431
Score = 46.9 bits (96), Expect(3) = 4e-031
Identities = 13/31 (41%), Positives = 24/31 (77%)
Frame = +2 / -2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP 373
WL+IG+ ++R+ W+ +V+YD F+++CN P
Sbjct: 328 WLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 236
>gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 3', mRNA sequence
Length = 525
Score = 70.3 bits (147), Expect(3) = 4e-031
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / -1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 441 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 331
Score = 55.1 bits (114), Expect(3) = 4e-031
Identities = 20/30 (66%), Positives = 24/30 (80%)
Frame = +2 / -1
Query: 86 WQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
WQ NENRLLWKLGTLP GL+TF++ + L
Sbjct: 519 WQTQNENRLLWKLGTLPPGLITFYSTTKPL 430
Score = 46.9 bits (96), Expect(3) = 4e-031
Identities = 13/31 (41%), Positives = 24/31 (77%)
Frame = +2 / -1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP 373
WL+IG+ ++R+ W+ +V+YD F+++CN P
Sbjct: 327 WLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 235
>gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_G13 5', mRNA sequence
Length = 523
Score = 65.2 bits (136), Expect(3) = 3e-028
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / +2
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 80 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 190
Score = 51.0 bits (105), Expect(3) = 3e-028
Identities = 17/24 (70%), Positives = 22/24 (91%)
Frame = +2 / +2
Query: 86 WQKLNENRLLWKLGTLPAGLVTFW 157
WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 2 WQNMNEDRVLWKLGTLPPGLMTFY 73
Score = 46.4 bits (95), Expect(3) = 3e-028
Identities = 12/30 (40%), Positives = 22/30 (73%)
Frame = +2 / +2
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNIN 370
WLE+ + KYR YW+ ++ YD +++ CN++
Sbjct: 194 WLELAMTKYRPYWTRYIKYDHPYIRGCNLS 283
>gb|AV833846.1|AV833846 AV833846 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags20p21, mRNA sequence
Length = 494
Score = 60.6 bits (126), Expect(4) = 1e-025
Identities = 20/31 (64%), Positives = 22/31 (70%)
Frame = +2 / +3
Query: 8 CGWAYGMNMFDLSGWRKQNITEVYHTWQKLN 100
C WAYGMN+FDL WRK NI + YH W K N
Sbjct: 96 CAWAYGMNVFDLEAWRKTNIRDTYHFWLKEN 188
Score = 51.0 bits (105), Expect(4) = 1e-025
Identities = 16/38 (42%), Positives = 25/38 (65%)
Frame = +1 / +3
Query: 163 HTFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
H +D SWH LGLGY + + + +++A V+H+NG K
Sbjct: 255 HVHGIDPSWHMLGLGYQESTDIESVKKAAVVHYNGQCK 368
Score = 29.9 bits (59), Expect(4) = 1e-025
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = +2 / +1
Query: 314 YWSAHVNYDQVFLQECNI 367
+W+ HVNY F++ C+I
Sbjct: 406 FWTKHVNYSNDFIRNCHI 459
Score = 29.5 bits (58), Expect(4) = 1e-025
Identities = 10/29 (34%), Positives = 14/29 (48%)
Frame = +2 / +3
Query: 89 QKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
+ L LWK GTLP L+ F + +
Sbjct: 183 ENLKAGLTLWKFGTLPPALIAFRGHVHGI 269
>gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 3', mRNA sequence
Length = 703
Score = 75.3 bits (158), Expect(4) = 2e-024
Identities = 25/52 (48%), Positives = 37/52 (71%)
Frame = +2 / -1
Query: 20 YGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
+GMN+ DL WR +N+T +YH WQ+ N + LWKLG+LP GL+ F+ + +L
Sbjct: 703 FGMNVLDLVEWRNKNVTGIYHYWQERNADHTLWKLGSLPPGLLAFYGLVEAL 548
Score = 40.0 bits (81), Expect(4) = 2e-024
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2 / -1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQEC 361
WL+IG+ KY+ +W +V+Y LQ C
Sbjct: 448 WLKIGMEKYKGFWDNYVDYSHPLLQRC 368
Score = 26.7 bits (52), Expect(4) = 2e-024
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +1 / -1
Query: 220 VNEKDIRRATVIHFNGNLK 276
V+ I+ V+H+NGNLK
Sbjct: 508 VDPDTIKEGAVLHYNGNLK 452
Score = 24.4 bits (47), Expect(4) = 2e-024
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +1 / -1
Query: 175 LDRSWHQLGLGY 210
L+ WH LGLGY
Sbjct: 550 LNPKWHVLGLGY 515
>gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. vulgare cDNA clone HM10N01
3-PRIME, mRNA sequence
Length = 535
Score = 70.3 bits (147), Expect(3) = 4e-023
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / -3
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 491 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 381
Score = 46.9 bits (96), Expect(3) = 4e-023
Identities = 13/31 (41%), Positives = 24/31 (77%)
Frame = +2 / -3
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP 373
WL+IG+ ++R+ W+ +V+YD F+++CN P
Sbjct: 377 WLDIGMNQFRQLWTKYVDYDDSFIRQCNFAP 285
Score = 28.1 bits (55), Expect(3) = 4e-023
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +2 / -3
Query: 122 LGTLPAGLVTFWNRIRSL 175
LGTLP GL+TF++ + L
Sbjct: 533 LGTLPPGLITFYSTTKPL 480
>gb|BJ549178.1|BJ549178 BJ549178 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 5', mRNA sequence
Length = 606
Score = 106 bits (226), Expect = 1e-022
Identities = 37/53 (69%), Positives = 44/53 (83%)
Frame = +2 / +1
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWN 160
+ACGWAYGMN FDL+ WR++ TE YH WQ NENRLLWKLGTLP GL+TF++
Sbjct: 448 NACGWAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLGTLPPGLITFYS 606
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 305,443,433
Number of Sequences: 312970
Number of extensions: 4317007
Number of successful extensions: 35854
Number of sequences better than 1.0e-020: 37
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7550174202
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)