TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3023663.2.1
         (613 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BI949868.1|BI949868  HVSMEl0016J08f Hordeum vulgare spike...   205   2e-052

>gb|BI949868.1|BI949868 HVSMEl0016J08f Hordeum vulgare spike EST library HVcDNA0012
           (Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
           clone HVSMEl0016J08f, mRNA sequence
          Length = 527

 Score =  205 bits (442), Expect = 2e-052
 Identities = 85/122 (69%), Positives = 96/122 (78%)
 Frame = -2 / +3

                                                                       
Query: 594 PETNRKDVNLPGILQSPISNMLRKVEKVSQDIPKHRKVTHPEYEVETANGRITKRRKTRS 415
           PET RK+ N+ G++ SPISN+LRKVEK SQD+P HRKVTH EYEVETANG+ITKR+KT+S
Sbjct: 54  PETKRKNANISGVIHSPISNVLRKVEKASQDVPNHRKVTHHEYEVETANGKITKRKKTKS 233

                                                                       
Query: 414 PVMFGEPNSQKPLHNXXXXXXXXXXXVPTRSRAHPANIGELFSEGSLNPYADDPYAFD*K 235
            VMFGEPN+QK L N           V  RSR HPANIGELFSEGSLNPYADDP+A  *K
Sbjct: 234 TVMFGEPNTQKSLQNTADKDVTKTRKVVARSRPHPANIGELFSEGSLNPYADDPFALG*K 413

             
Query: 234 LT 229
           LT
Sbjct: 414 LT 419




 Score =  191 bits (412), Expect = 3e-048
 Identities = 89/122 (72%), Positives = 93/122 (76%)
 Frame = +2 / -2

                                                                       
Query: 230 VSF*SKA*GSSAYGFKEPSENNSPMFAGCARDLVGTFFSFLTSLSVVLCNGF*EFGSPNM 409
           VSF*  A GSSAYGFKEPSENNSP+FAG  RDL  TF  F+TSLS V CN F* FGSPNM
Sbjct: 418 VSF*PNAKGSSAYGFKEPSENNSPIFAGWGRDLATTFLVFVTSLSAVFCNDF*VFGSPNM 239

                                                                       
Query: 410 TGLLVFLRFVILPFAVSTSYSGWVTFLCLGIS*DTFSTFLNMFEIGDCKIPGKFTSFRLV 589
           T LLVFLRFVI PFAVSTSYS  VTFL LG S*  FSTFL+  EIG+C  P  F  FRLV
Sbjct: 238 TVLLVFLRFVIFPFAVSTSYSWCVTFL*LGTS*LAFSTFLSTLEIGECITPDMFAFFRLV 59

             
Query: 590 SG 595
           SG
Sbjct: 58  SG 53




 Score =  177 bits (380), Expect = 7e-044
 Identities = 81/134 (60%), Positives = 96/134 (71%)
 Frame = +1 / -1

                                                                       
Query: 211 KNDTSRRELLVKSIRVVSIRIQGTLRE*FTNVCRMCTGSCRNLF*FFNIFVSSIMQWFLR 390
           K  T+RRELL +  RV+SI IQG L +*F+++CRM TGSC NL  F NI VSSI+Q  L 
Sbjct: 437 KISTNRRELLAQCKRVISIWIQGALGK*FSDICRMGTGSCNNLSCFCNILVSSILQ*LLS 258

                                                                       
Query: 391 VRFTKHDRAPSFPALCDSSICCLNFVFRVGYLSMFRNILRHFLYLSQHVRNW*L*NPR*V 570
           VRF KHD   SF ALCD SICCL+F+F V YLS+ RNIL  FLYL Q+VRNW + N R V
Sbjct: 257 VRFPKHDSTLSFLALCDFSICCLHFIFMVCYLSVIRNILTCFLYLPQYVRNWRVYNSRYV 78

                         
Query: 571 YVLPIGLRTYQLDL 612
            +LP GLRT+   L
Sbjct: 77  CILPFGLRTWTWQL 36




 Score =  161 bits (347), Expect = 2e-039
 Identities = 77/123 (62%), Positives = 85/123 (69%)
 Frame = -1 / +2

                                                                       
Query: 598 KSGDQSEGRKLTWDFTITNFEHVEKGRESVSGYS*T*KGNPP*IRS*DSKWKNHKAQEN* 419
           +S DQ+E  K  W +T+ NF   E+GRES SG S*+ KGN P*I S DSKWKNHKAQEN*
Sbjct: 50  RS*DQTEECKHIWSYTLANF*RTEEGRESKSGCS*SQKGNTP*I*SGDSKWKNHKAQEN* 229

                                                                       
Query: 418 EPCHVW*TELSETIA*YY*QRC*KTKKGSYKIPCTSCKHW*IIL*GFLESVC*RPLCF*L 239
           E CHVW TE SE IA Y *Q C K KKG  KIP  SCK+  II  G LES+C* P C  L
Sbjct: 230 EYCHVWGTEHSEVIAKYC*QGCYKNKKGCCKIPSPSCKYRRIIFRGLLESIC**PFCIGL 409

              
Query: 238 EAH 230
           EAH
Sbjct: 410 EAH 418




 Score =  152 bits (327), Expect = 1e-036
 Identities = 77/125 (61%), Positives = 86/125 (68%)
 Frame = +3 / -3

                                                                       
Query: 225 QA*ASSQKHKGRQHTDSRNPQRIIHQCLQDVHGIL*EPFLVF*HLCQ*YYAMVSESSVHQ 404
           Q *ASS   KG QH DSR+P++II + LQD  GIL +PFL  *H CQ Y+AM SE SV Q
Sbjct: 423 QT*ASSPMQKGHQHMDSRSPRKIILRYLQDGDGILQQPFLFL*HPCQQYFAMTSECSVPQ 244

                                                                       
Query: 405 T*QGS*FSCAL*FFHLLSQLRIQGGLPFYV*EYPETLSLPFSTCSKLVIVKSQVSLRPSD 584
           T*Q S*FSCAL*FFHLLS L I G LPF  *E+P+ LSLP S   KL  V  Q+ L  S 
Sbjct: 243 T*QYS*FSCAL*FFHLLSPLHIHGVLPFCD*EHPDLLSLPSSVR*KLASV*LQICLHSSV 64

                
Query: 585 WSPDL 599
           WS DL
Sbjct: 63  WSQDL 49




 Score =  137 bits (293), Expect = 7e-032
 Identities = 69/109 (63%), Positives = 77/109 (70%)
 Frame = -3 / +1

                                                                       
Query: 596 VRRPIGRT*TYLGFYNHQFRTC*ER*RKCLRIFLNIER*PTLNTKLRQQMEESQSAGKLG 417
           V RP GR  TYL  Y  QF T * R*RK +R+FL  ER* T+N K RQQME+SQSA KL 
Sbjct: 52  VLRPNGRMQTYLELYTRQFLTY*GR*RKQVRMFLITER*HTMNMKWRQQMEKSQSARKLR 231

                                                            
Query: 416 ALSCLVNRTLRNHCIILLTKMLKN*KRFLQDPVHILQTLVNYSLRVP*I 270
            LSCL NRTLR+HC ILLT+ML+  +R LQDPV ILQ   NY  R P*I
Sbjct: 232 VLSCLGNRTLRSHCKILLTRMLQKQERLLQDPVPILQISENYFPRAP*I 378



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 284,019,139
Number of Sequences: 312970
Number of extensions: 4209914
Number of successful extensions: 39683
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6447339768
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)