TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950292.2.1
(1205 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB878751.1|CB878751 HP09L01T HP Hordeum vulgare subsp. v... 520 e-147
gb|BU986719.1|BU986719 HF12L11r HF Hordeum vulgare subsp. v... 436 e-126
gb|CK122559.1|CK122559 BES1824103p10 BES1824 Hordeum vulgar... 387 e-112
gb|AV916883.1|AV916883 AV916883 K. Sato unpublished cDNA li... 323 1e-092
gb|BI778984.2|BI778984 EBro01_SQ002_G21_R root, 3 week, hyd... 300 6e-086
gb|BE454747.1|BE454747 HVSMEh0095M02f Hordeum vulgare 5-45 ... 269 7e-077
gb|AV922181.1|AV922181 AV922181 K. Sato unpublished cDNA li... 264 2e-075
gb|BU998909.1|BU998909 HI12I16r HI Hordeum vulgare subsp. v... 265 2e-075
gb|BI956222.1|BI956222 HVSMEn0001E03f Hordeum vulgare rachi... 185 1e-050
gb|BE215119.2|BE215119 HV_CEb0005N21f Hordeum vulgare seedl... 93 8e-046
gb|CB860069.1|CB860069 HI12I16w HI Hordeum vulgare subsp. v... 153 8e-042
>gb|CB878751.1|CB878751 HP09L01T HP Hordeum vulgare subsp. vulgare cDNA clone HP09L01
5-PRIME, mRNA sequence
Length = 696
Score = 520 bits (1130), Expect = e-147
Identities = 208/220 (94%), Positives = 216/220 (98%)
Frame = +2 / +1
Query: 170 RIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLVFAT 349
RIVKWQQ DIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAF+VILILFLVFAT
Sbjct: 37 RIVKWQQMDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFIVILILFLVFAT 216
Query: 350 SAYCAAYGESASHAALTITSVVFLHFLFAGIVLATLCWFLTNSYLREEPNSHVVEQRVEW 529
SAYCAA+GESA HAALTI SVVF+HFLFAG+VLATLCWFLTNSYLREEPNSHVVEQRVEW
Sbjct: 217 SAYCAAFGESAPHAALTIISVVFVHFLFAGLVLATLCWFLTNSYLREEPNSHVVEQRVEW 396
Query: 530 LYAFDVHCNSFFPAFVILYVVQYFLSPLLVAHGFFPALLSNLLFVVAISYYHYLNFLGYD 709
LYAFDVHCNSFFPAFVILYV+QYFLSPLL+AHGF PALLSNLLF+VAISYYHYLNFLGYD
Sbjct: 397 LYAFDVHCNSFFPAFVILYVLQYFLSPLLIAHGFLPALLSNLLFMVAISYYHYLNFLGYD 576
Query: 710 VLPFLDRTTFFLYPIGLVIILSPLMILIGFNPTRYFLSLY 829
VLPFLDRTTFFLYPIGLVIILSPLMIL+GFNPTRYFLSLY
Sbjct: 577 VLPFLDRTTFFLYPIGLVIILSPLMILVGFNPTRYFLSLY 696
Score = 432 bits (938), Expect = e-120
Identities = 187/219 (85%), Positives = 199/219 (90%)
Frame = -3 / -2
Query: 828 YKLRKYLVGLNPISIISGDRMMTRPIGYRKNVVLSRNGRTSYPRKFR****EIATTKSRL 649
YKLRKY VGLNP SI+SGDRMMT PIGY+KNVV S+NGRTSYPRKFR****EIAT R
Sbjct: 695 YKLRKYRVGLNPTSIMSGDRMMTSPIGYKKNVVRSKNGRTSYPRKFR****EIATMNRRF 516
Query: 648 DNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA*SHSTRCSTTWLLGSSLK*EFV 469
DN+AGK PCA NRGD+KY +TYRMTNAGKNELQ*TSNA SHSTRCSTTWLLGSSL+*E V
Sbjct: 515 DNRAGKNPCAINRGDKKY*STYRMTNAGKNELQ*TSNAYSHSTRCSTTWLLGSSLR*ELV 336
Query: 468 RNQQSVAKTIPANKKWRNTTEVIVRAACEALSPYAAQ*ADVANTRKRIRITTNAGSSRAH 289
RNQQSVAKT PANKKW NTTE+IV+AA ALSP AAQ*ADVANTRKRIR+T NAGSSRAH
Sbjct: 335 RNQQSVAKTSPANKKWTNTTEMIVKAAWGALSPNAAQ*ADVANTRKRIRMTINAGSSRAH 156
Query: 288 WFFVCL*YLVC**TTFGEVQR*TICQNVYSISLCCHLTM 172
WFFVCL*YLVC *TTFGEVQR*TICQNVYSIS+CCHLT+
Sbjct: 155 WFFVCL*YLVCW*TTFGEVQR*TICQNVYSISICCHLTI 39
Score = 347 bits (751), Expect = 1e-094
Identities = 149/230 (64%), Positives = 179/230 (77%)
Frame = -2 / -1
Query: 829 VQA*KVSCRIKPY*YHKWRQDDDETNRIQEECCXXXXXXXXXXXKVQVVIIRNCHHEE*I 650
VQA KVSC IKPY YH+WR +DD++NR+QE+CC +VQ+VIIRNCHHE+ +
Sbjct: 696 VQAQKVSCWIKPYEYHEWR*NDDKSNRVQEKCCSVQKWKNIIS*EVQIVIIRNCHHEQEV 517
Query: 649 R*QSWKEAMRDQ*RRQKILHHI*DDKRRKERVTMNVKCIEPLHALLNNMAVRFLSQVRIC 470
* +E+M DQ RRQK+L HI*DD+ R+ERV +N+KCI+PL L ++MA+RFL Q+RI
Sbjct: 516 ***GRQESMCDQQRRQKVLKHI*DDECREERVAVNIKCIQPLDTLFDHMAIRFLPQIRIG 337
Query: 469 KEPAKCGQNYPSKQEMEKHNRSDC*SSM*GTLSICCTVS*CCKHEEKNQNNNKCRIITGP 290
K+PAKCGQN PSKQEM+KHNR DC*SSM TLS CTVS*C KH+EKN N+NKCRII P
Sbjct: 336 KKPAKCGQN*PSKQEMDKHNRDDC*SSMGRTLSKRCTVS*CRKHKEKN*NDNKCRIIPSP 157
Query: 289 LVLCLLVIFGVLIDYFW*STEMNHLPKCVFNIPLLPLDDAAEVKKKTPPG 140
LVLCLLVIFGVL+DYFW*ST+MNHLPK VF+I LLPLD+ P G
Sbjct: 156 LVLCLLVIFGVLVDYFW*STKMNHLPKRVFDIHLLPLDNTVGDTDLQPRG 7
Score = 286 bits (618), Expect = 2e-076
Identities = 137/220 (62%), Positives = 154/220 (70%)
Frame = -1 / -3
Query: 827 TSLESILSD*TLLVS*VETG**RDQSDTGRMLFYLEMEEHHILESSGSDNKKLPPRRVD* 648
TS ESI+ D*TL VS*V **+ QS T +MLF +MEEHHIL SS SDNKKLPP
Sbjct: 694 TSSESIVLD*TLRVS*VAIE**QVQSGTRKMLFGPKMEEHHILGSSDSDNKKLPP*TGGL 515
Query: 647 ITKLERSHARPIEATENTAPHIG*QTQERTSYNERQMHRATPRVAQQHGC*VPLSSKNL* 468
I R H R EAT++ HIG*+ Q RTS +E QMH AT + HG *VP S KN *
Sbjct: 514 IIGQARIHVRSTEATKSIEAHIG*RMQGRTSCSEHQMHTATRHAVRPHGY*VPPSDKNW* 335
Query: 467 GTSKVWPKLSQQTRNGETQPK*LLEQHVRXXXXXXXXXXXLQTRGKESE*QQMQDHHGPT 288
TSKVWPKL+QQTRNG+TQP+*LL+QH QT+GKE E*Q MQDH PT
Sbjct: 334 ETSKVWPKLAQQTRNGQTQPR*LLKQHGAHSLQTLHSKLMSQTQGKELE*Q*MQDHPEPT 155
Query: 287 GSLFACDIWCADRLLLVKYRDEPFAKMCIQYPFVAT*RCG 168
GSLFACDIWCA RLLLVKY+DEPFAK CI+YPF AT*+ G
Sbjct: 154 GSLFACDIWCAGRLLLVKYKDEPFAKTCIRYPFAAT*QYG 35
Score = 282 bits (611), Expect(2) = 2e-082
Identities = 119/176 (67%), Positives = 135/176 (76%)
Frame = +1 / +3
Query: 169 PHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPRVCN 348
P+ QVA GY IH+LANGSSLYFTKSSL AHQISQANKEPVG *SCI C+S+SFP VC+
Sbjct: 36 PYCQVAANGYRIHVLANGSSLYFTKSSLPAHQISQANKEPVGSG*SCIYCHSNSFPCVCD 215
Query: 349 ISLLCSIWRECLTCCSNNHFGCVSPFLVCWDSFGHTLLVPYKFLLERGT*QPCC*ATRGV 528
ISLLCS+WREC CC NNH GCV PFLVCW SFGHTLLV Y+FL E GT* PC V
Sbjct: 216 ISLLCSVWRECAPCCFNNHLGCVCPFLVCWASFGHTLLVSYQFLSEGGT**PCGRTACRV 395
Query: 529 ALCI*RSL*LVLSCVCHPICGAVFSVASIGRAWLLSSFVI*STLRGGNFLLSLPEL 696
A+CI* SL LVL C+ HPIC ++ VAS+ R W+L+ +I + GGNFLLSL EL
Sbjct: 396 AVCI*CSLQLVLPCIRHPICASILFVASVDRTWILACPIIKPPVHGGNFLLSLSEL 563
Score = 169 bits (364), Expect(2) = 5e-059
Identities = 87/157 (55%), Positives = 92/157 (58%)
Frame = +3 / +2
Query: 357 TVQHMERVPHMLL*QSLRLCFSISCLLG*FWPHFAGSLQILT*ERNLTAMLLSNAWSGSM 536
TVQ +ERV MLL*QS RLC SISCLLG*FWPHFAG L IL * RNL AM ++ SG M
Sbjct: 224 TVQRLERVRPMLL*QSSRLCLSISCLLG*FWPHFAGFLPILI*GRNLIAMWSNSVSSGCM 403
Query: 537 HLTFIVTRSFLRLSSYMWCSIFCRLYWSRMASFQLCYLIYSSWWQFLIIXXXXXXXXXXX 716
HL F TRS L SSYM + FCRL S M S Y SWWQFLII
Sbjct: 404 HLMFTATRSSLHSSSYMCFNTFCRLC*SHMDSCLPYYQTSCSWWQFLIITI*TS*DMMFF 583
Query: 717 HF*IEQHXXXXXXXXXXXXXHL*Y**GLIRQDTF*AC 827
HF EQH H *Y *GLI+ DTF*AC
Sbjct: 584 HFWTEQHFSCTRLDLSSFYRHS*YS*GLIQHDTF*AC 694
Score = 79.4 bits (167), Expect(2) = 5e-059
Identities = 37/55 (67%), Positives = 39/55 (70%)
Frame = +3 / +2
Query: 141 PGGVFFFTSAASSSGNKGILNTHFGKWFISVLHQK*SISTPNITSKQRTSGPVMI 305
P G + SSG+K I NT FGKWFI VLHQK*S STPNITSKQRTSG MI
Sbjct: 8 PRGCRSVSPTVLSSGSKWISNTRFGKWFIFVLHQK*STSTPNITSKQRTSGLGMI 172
Score = 44.1 bits (90), Expect(2) = 2e-082
Identities = 17/28 (60%), Positives = 23/28 (82%)
Frame = +1 / +3
Query: 745 VSDWSRHHPVSTYDTNRV*SDKILSKLV 828
V DW+ HH ++T+DT RV*S+ ILS+LV
Sbjct: 612 VPDWTCHHSIATHDTRRV*SNTILSELV 695
>gb|BU986719.1|BU986719 HF12L11r HF Hordeum vulgare subsp. vulgare cDNA clone HF12L11
5-PRIME, mRNA sequence
Length = 652
Score = 436 bits (946), Expect(2) = e-126
Identities = 173/184 (94%), Positives = 180/184 (97%)
Frame = +2 / +3
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRIVKWQQ DIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAF+VILILFLV
Sbjct: 99 YLRRIVKWQQMDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFIVILILFLV 278
Query: 341 FATSAYCAAYGESASHAALTITSVVFLHFLFAGIVLATLCWFLTNSYLREEPNSHVVEQR 520
FATSAYCAA+GESA HAALTI SVVF+HFLFAG+VLATLCWFLTNSYLREEPNSHVVEQR
Sbjct: 279 FATSAYCAAFGESAPHAALTIISVVFVHFLFAGLVLATLCWFLTNSYLREEPNSHVVEQR 458
Query: 521 VEWLYAFDVHCNSFFPAFVILYVVQYFLSPLLVAHGFFPALLSNLLFVVAISYYHYLNFL 700
VEWLYAFDVHCNSFFPAFVILYV+QYFLSPLL+AHGF PALLSNLLF+VAISYYHYLNFL
Sbjct: 459 VEWLYAFDVHCNSFFPAFVILYVLQYFLSPLLIAHGFLPALLSNLLFMVAISYYHYLNFL 638
Query: 701 GYDV 712
GYDV
Sbjct: 639 GYDV 650
Score = 358 bits (776), Expect(2) = e-102
Identities = 156/181 (86%), Positives = 165/181 (91%)
Frame = -3 / -1
Query: 714 RTSYPRKFR****EIATTKSRLDNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA 535
RTSYPRKFR****EIAT R DN+AGK PCA NRGD+KY +TYRMTNAGKNELQ*TSNA
Sbjct: 652 RTSYPRKFR****EIATMNRRFDNRAGKNPCAINRGDKKY*STYRMTNAGKNELQ*TSNA 473
Query: 534 *SHSTRCSTTWLLGSSLK*EFVRNQQSVAKTIPANKKWRNTTEVIVRAACEALSPYAAQ* 355
SHSTRCSTTWLLGSSL+*E VRNQQSVAKT PANKKW NTTE+IV+AA ALSP AAQ*
Sbjct: 472 YSHSTRCSTTWLLGSSLR*ELVRNQQSVAKTSPANKKWTNTTEMIVKAAWGALSPNAAQ* 293
Query: 354 ADVANTRKRIRITTNAGSSRAHWFFVCL*YLVC**TTFGEVQR*TICQNVYSISLCCHLT 175
ADVANTRKRIR+T NAGSSRAHWFFVCL*YLVC *TTFGEVQR*TICQNVYSIS+CCHLT
Sbjct: 292 ADVANTRKRIRMTINAGSSRAHWFFVCL*YLVCW*TTFGEVQR*TICQNVYSISICCHLT 113
Query: 174 M 172
+
Sbjct: 112 I 110
Score = 304 bits (658), Expect = 7e-082
Identities = 130/192 (67%), Positives = 157/192 (81%)
Frame = -2 / -3
Query: 694 VQVVIIRNCHHEE*IR*QSWKEAMRDQ*RRQKILHHI*DDKRRKERVTMNVKCIEPLHAL 515
VQ+VIIRNCHHE+ + * +E+M DQ RRQK+L HI*DD+ R+ERV +N+KCI+PL L
Sbjct: 632 VQIVIIRNCHHEQEV***GRQESMCDQQRRQKVLKHI*DDECREERVAVNIKCIQPLDTL 453
Query: 514 LNNMAVRFLSQVRICKEPAKCGQNYPSKQEMEKHNRSDC*SSM*GTLSICCTVS*CCKHE 335
++MA+RFL Q+RI K+PAKCGQN PSKQEM+KHNR DC*SSM TLS CTVS*C KH+
Sbjct: 452 FDHMAIRFLPQIRIGKKPAKCGQN*PSKQEMDKHNRDDC*SSMGRTLSKRCTVS*CRKHK 273
Query: 334 EKNQNNNKCRIITGPLVLCLLVIFGVLIDYFW*STEMNHLPKCVFNIPLLPLDDAAEVKK 155
EKN N+NKCRII PLVLCLLVIFGVL+DYFW*ST+MNHLPK VF+I LLPLD+ AE++
Sbjct: 272 EKN*NDNKCRIIPSPLVLCLLVIFGVLVDYFW*STKMNHLPKRVFDIHLLPLDNTAEIRT 93
Query: 154 KTPPGRSGRPRA 119
+ G +G P A
Sbjct: 92 EERRGGAGGPPA 57
Score = 286 bits (618), Expect = 2e-076
Identities = 120/177 (67%), Positives = 136/177 (76%)
Frame = +1 / +2
Query: 166 PPHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPRVC 345
PP+ QVA GY IH+LANGSSLYFTKSSL AHQISQANKEPVG *SCI C+S+SFP VC
Sbjct: 104 PPYCQVAANGYRIHVLANGSSLYFTKSSLPAHQISQANKEPVGSG*SCIYCHSNSFPCVC 283
Query: 346 NISLLCSIWRECLTCCSNNHFGCVSPFLVCWDSFGHTLLVPYKFLLERGT*QPCC*ATRG 525
+ISLLCS+WREC CC NNH GCV PFLVCW SFGHTLLV Y+FL E GT* PC
Sbjct: 284 DISLLCSVWRECAPCCFNNHLGCVCPFLVCWASFGHTLLVSYQFLSEGGT**PCGRTACR 463
Query: 526 VALCI*RSL*LVLSCVCHPICGAVFSVASIGRAWLLSSFVI*STLRGGNFLLSLPEL 696
VA+CI* SL LVL C+ HPIC ++ VAS+ R W+L+ +I + GGNFLLSL EL
Sbjct: 464 VAVCI*CSLQLVLPCIRHPICASILFVASVDRTWILACPIIKPPVHGGNFLLSLSEL 634
Score = 256 bits (553), Expect = 2e-067
Identities = 117/199 (58%), Positives = 131/199 (65%)
Frame = -1 / -2
Query: 713 EHHILESSGSDNKKLPPRRVD*ITKLERSHARPIEATENTAPHIG*QTQERTSYNERQMH 534
EHHIL SS SDNKKLPP I R H R EAT++ HIG*+ Q RTS +E QMH
Sbjct: 651 EHHILGSSDSDNKKLPP*TGGLIIGQARIHVRSTEATKSIEAHIG*RMQGRTSCSEHQMH 472
Query: 533 RATPRVAQQHGC*VPLSSKNL*GTSKVWPKLSQQTRNGETQPK*LLEQHVRXXXXXXXXX 354
AT + HG *VP S KN * TSKVWPKL+QQTRNG+TQP+*LL+QH
Sbjct: 471 TATRHAVRPHGY*VPPSDKNW*ETSKVWPKLAQQTRNGQTQPR*LLKQHGAHSLQTLHSK 292
Query: 353 XXLQTRGKESE*QQMQDHHGPTGSLFACDIWCADRLLLVKYRDEPFAKMCIQYPFVAT*R 174
QT+GKE E*Q MQDH PTGSLFACDIWCA RLLLVKY+DEPFAK CI+YPF AT*+
Sbjct: 291 LMSQTQGKELE*Q*MQDHPEPTGSLFACDIWCAGRLLLVKYKDEPFAKTCIRYPFAAT*Q 112
Query: 173 CGGGKKKNTPRAEREAPRP 117
GG + P P
Sbjct: 111 YGGDTDRRAAGRSGRPPGP 55
Score = 142 bits (304), Expect(2) = 8e-052
Identities = 69/109 (63%), Positives = 73/109 (66%)
Frame = +3 / +1
Query: 357 TVQHMERVPHMLL*QSLRLCFSISCLLG*FWPHFAGSLQILT*ERNLTAMLLSNAWSGSM 536
TVQ +ERV MLL*QS RLC SISCLLG*FWPHFAG L IL * RNL AM ++ SG M
Sbjct: 295 TVQRLERVRPMLL*QSSRLCLSISCLLG*FWPHFAGFLPILI*GRNLIAMWSNSVSSGCM 474
Query: 537 HLTFIVTRSFLRLSSYMWCSIFCRLYWSRMASFQLCYLIYSSWWQFLII 683
HL F TRS L SSYM + FCRL S M S Y SWWQFLII
Sbjct: 475 HLMFTATRSSLHSSSYMCFNTFCRLC*SHMDSCLPYYQTSCSWWQFLII 621
Score = 82.6 bits (174), Expect(2) = 8e-052
Identities = 41/62 (66%), Positives = 42/62 (67%)
Frame = +3 / +1
Query: 120 ARGLPLRPGGVFFFTSAASSSGNKGILNTHFGKWFISVLHQK*SISTPNITSKQRTSGPV 299
A G P P SA SSG+K I NT FGKWFI VLHQK*S STPNITSKQRTSG
Sbjct: 58 AGGPPAPPRRSSVRISAVLSSGSKWISNTRFGKWFIFVLHQK*STSTPNITSKQRTSGLG 237
Query: 300 MI 305
MI
Sbjct: 238 MI 243
Score = 38.2 bits (77), Expect(2) = e-126
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +1 / +3
Query: 94 MLPTTASKGRGASRSARGVF 153
MLPTTASKGRGA+RSA +F
Sbjct: 33 MLPTTASKGRGAARSAPPLF 92
Score = 35.9 bits (72), Expect(2) = e-102
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = -1 / -1
Query: 140 AEREAPRPLDAVVGSI 93
AER APRPLDAVVGSI
Sbjct: 79 AERAAPRPLDAVVGSI 32
>gb|CK122559.1|CK122559 BES1824103p10 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010P103 5-PRIME, mRNA sequence
Length = 694
Score = 387 bits (840), Expect(2) = e-112
Identities = 154/165 (93%), Positives = 159/165 (96%)
Frame = +2 / +2
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRIVKWQQ DIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAF+VILILFLV
Sbjct: 152 YLRRIVKWQQMDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFIVILILFLV 331
Query: 341 FATSAYCAAYGESASHAALTITSVVFLHFLFAGIVLATLCWFLTNSYLREEPNSHVVEQR 520
FATSAYCAA+GESA HAALTI SVVF HFLFAG+VLATLCWFLTNSYLREEPNSHVVEQR
Sbjct: 332 FATSAYCAAFGESAPHAALTIISVVFXHFLFAGLVLATLCWFLTNSYLREEPNSHVVEQR 511
Query: 521 VEWLYAFDVHCNSFFPAFVILYVVQYFLSPLLVAHGFFPALLSNL 655
VEWLYAFDVHCNSFFPAFVILYV+QYFLSPL +AHGF PALLSNL
Sbjct: 512 VEWLYAFDVHCNSFFPAFVILYVLQYFLSPLXIAHGFLPALLSNL 646
Score = 323 bits (700), Expect(2) = 4e-092
Identities = 138/161 (85%), Positives = 147/161 (91%)
Frame = -3 / -2
Query: 654 RLDNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA*SHSTRCSTTWLLGSSLK*E 475
R DN+AGK PCA RGD+KY +TYRMTNAGKNELQ*TSNA SHSTRCSTTWLLGSSL+*E
Sbjct: 645 RFDNRAGKNPCAIXRGDKKY*STYRMTNAGKNELQ*TSNAYSHSTRCSTTWLLGSSLR*E 466
Query: 474 FVRNQQSVAKTIPANKKWRNTTEVIVRAACEALSPYAAQ*ADVANTRKRIRITTNAGSSR 295
VRNQQSVAKT PANKKW NTTE+IV+AA ALSP AAQ*ADVANTRKRIR+T NAGSSR
Sbjct: 465 LVRNQQSVAKTSPANKKWXNTTEMIVKAAWGALSPNAAQ*ADVANTRKRIRMTINAGSSR 286
Query: 294 AHWFFVCL*YLVC**TTFGEVQR*TICQNVYSISLCCHLTM 172
AHWFFVCL*YLVC *TTFGEVQR*TICQNVYSIS+CCHLT+
Sbjct: 285 AHWFFVCL*YLVCW*TTFGEVQR*TICQNVYSISICCHLTI 163
Score = 274 bits (593), Expect = 6e-073
Identities = 117/172 (68%), Positives = 140/172 (81%)
Frame = -2 / -1
Query: 634 KEAMRDQ*RRQKILHHI*DDKRRKERVTMNVKCIEPLHALLNNMAVRFLSQVRICKEPAK 455
+E+M D RRQK+L HI*DD+ R+ERV +N+KCI+PL L ++MA+RFL Q+RI K+PAK
Sbjct: 625 QESMCDXQRRQKVLKHI*DDECREERVAVNIKCIQPLDTLFDHMAIRFLPQIRIGKKPAK 446
Query: 454 CGQNYPSKQEMEKHNRSDC*SSM*GTLSICCTVS*CCKHEEKNQNNNKCRIITGPLVLCL 275
CGQN PSKQEM KHNR DC*SSM TLS CTVS*C KH+EKN N+NKCRII PLVLCL
Sbjct: 445 CGQN*PSKQEMXKHNRDDC*SSMGRTLSKRCTVS*CRKHKEKN*NDNKCRIIPSPLVLCL 266
Query: 274 LVIFGVLIDYFW*STEMNHLPKCVFNIPLLPLDDAAEVKKKTPPGRSGRPRA 119
LVIFGVL+DYFW*ST+MNHLPK VF+I LLPLD+ AE++ + G +G P A
Sbjct: 265 LVIFGVLVDYFW*STKMNHLPKRVFDIHLLPLDNTAEIRTEERRGGAGGPPA 110
Score = 264 bits (572), Expect(2) = 2e-070
Identities = 110/161 (68%), Positives = 124/161 (77%)
Frame = +1 / +1
Query: 166 PPHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPRVC 345
PP+ QVA GY IH+LANGSSLYFTKSSL AHQISQANKEPVG *SCI C+S+SFP VC
Sbjct: 157 PPYCQVAANGYRIHVLANGSSLYFTKSSLPAHQISQANKEPVGSG*SCIYCHSNSFPCVC 336
Query: 346 NISLLCSIWRECLTCCSNNHFGCVSPFLVCWDSFGHTLLVPYKFLLERGT*QPCC*ATRG 525
+ISLLCS+WREC CC NNH GCV PFLVCW SFGHTLLV Y+FL E GT* PC
Sbjct: 337 DISLLCSVWRECAPCCFNNHLGCVXPFLVCWASFGHTLLVSYQFLSEGGT**PCGRTACR 516
Query: 526 VALCI*RSL*LVLSCVCHPICGAVFSVASIGRAWLLSSFVI 648
VA+CI* SL LVL C+ HPIC ++ VAS R W+L+ +I
Sbjct: 517 VAVCI*CSLQLVLPCIRHPICASILFVASXDRTWILACPII 639
Score = 227 bits (490), Expect = 1e-058
Identities = 101/172 (58%), Positives = 115/172 (66%)
Frame = -1 / -3
Query: 632 RSHARPIEATENTAPHIG*QTQERTSYNERQMHRATPRVAQQHGC*VPLSSKNL*GTSKV 453
R H R EAT++ HIG*+ Q RTS +E QMH AT + HG *VP S KN * TSKV
Sbjct: 623 RIHVRSXEATKSIEAHIG*RMQGRTSCSEHQMHTATRHAVRPHGY*VPPSDKNW*ETSKV 444
Query: 452 WPKLSQQTRNGETQPK*LLEQHVRXXXXXXXXXXXLQTRGKESE*QQMQDHHGPTGSLFA 273
WPKL+QQTRNG+TQP+*LL+QH QT+GKE E*Q MQDH PTGSLFA
Sbjct: 443 WPKLAQQTRNGQTQPR*LLKQHGAHSLQTLHSKLMSQTQGKELE*Q*MQDHPEPTGSLFA 264
Query: 272 CDIWCADRLLLVKYRDEPFAKMCIQYPFVAT*RCGGGKKKNTPRAEREAPRP 117
CDIWCA RLLLVKY+DEPFAK CI+YPF AT*+ GG + P P
Sbjct: 263 CDIWCAGRLLLVKYKDEPFAKTCIRYPFAAT*QYGGDTDRRAAGRSGRPPGP 108
Score = 122 bits (260), Expect(2) = 9e-046
Identities = 59/92 (64%), Positives = 63/92 (68%)
Frame = +3 / +3
Query: 357 TVQHMERVPHMLL*QSLRLCFSISCLLG*FWPHFAGSLQILT*ERNLTAMLLSNAWSGSM 536
TVQ +ERV MLL*QS RLC ISCLLG*FWPHFAG L IL * RNL AM ++ SG M
Sbjct: 348 TVQRLERVRPMLL*QSSRLCLXISCLLG*FWPHFAGFLPILI*GRNLIAMWSNSVSSGCM 527
Query: 537 HLTFIVTRSFLRLSSYMWCSIFCRLYWSRMAS 632
HL F TRS L SSYM + FCRL S M S
Sbjct: 528 HLMFTATRSSLHSSSYMCFNTFCRLX*SHMDS 623
Score = 82.6 bits (174), Expect(2) = 9e-046
Identities = 41/62 (66%), Positives = 42/62 (67%)
Frame = +3 / +3
Query: 120 ARGLPLRPGGVFFFTSAASSSGNKGILNTHFGKWFISVLHQK*SISTPNITSKQRTSGPV 299
A G P P SA SSG+K I NT FGKWFI VLHQK*S STPNITSKQRTSG
Sbjct: 111 AGGPPAPPRRSSVRISAVLSSGSKWISNTRFGKWFIFVLHQK*STSTPNITSKQRTSGLG 290
Query: 300 MI 305
MI
Sbjct: 291 MI 296
Score = 38.2 bits (77), Expect(2) = e-112
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +1 / +2
Query: 94 MLPTTASKGRGASRSARGVF 153
MLPTTASKGRGA+RSA +F
Sbjct: 86 MLPTTASKGRGAARSAPPLF 145
Score = 35.9 bits (72), Expect(2) = 4e-092
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = -1 / -2
Query: 140 AEREAPRPLDAVVGSI 93
AER APRPLDAVVGSI
Sbjct: 132 AERAAPRPLDAVVGSI 85
Score = 22.1 bits (42), Expect(2) = 2e-070
Identities = 11/41 (26%), Positives = 15/41 (36%)
Frame = +3 / +1
Query: 30 AGASAIPEQTRGVXXXXXXXXXXXXXRVQGARGLPLRPGGV 152
A + A ++ G VQG G PLRP +
Sbjct: 22 AASXAADRESGGAAEGRRGENDAAHDGVQGPGGRPLRPAAL 144
>gb|AV916883.1|AV916883 AV916883 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19p08 5', mRNA sequence
Length = 528
Score = 323 bits (699), Expect(2) = 1e-092
Identities = 127/134 (94%), Positives = 131/134 (97%)
Frame = +2 / +3
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRIVKWQQ DIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAF+VILILFLV
Sbjct: 126 YLRRIVKWQQMDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFIVILILFLV 305
Query: 341 FATSAYCAAYGESASHAALTITSVVFLHFLFAGIVLATLCWFLTNSYLREEPNSHVVEQR 520
FATSAYCAA+GESA HAALTI SVVF+HFLFAG+VLATLCWFLTNSYLREEPNSHVVEQR
Sbjct: 306 FATSAYCAAFGESAPHAALTIISVVFVHFLFAGLVLATLCWFLTNSYLREEPNSHVVEQR 485
Query: 521 VEWLYAFDVHCNSF 562
VEWLYAFDVHCNSF
Sbjct: 486 VEWLYAFDVHCNSF 527
Score = 264 bits (572), Expect(2) = 1e-074
Identities = 115/130 (88%), Positives = 121/130 (93%)
Frame = -3 / -3
Query: 561 NELQ*TSNA*SHSTRCSTTWLLGSSLK*EFVRNQQSVAKTIPANKKWRNTTEVIVRAACE 382
NELQ*TSNA SHSTRCSTTWLLGSSL+*E VRNQQSVAKT PANKKW NTTE+IV+AA
Sbjct: 526 NELQ*TSNAYSHSTRCSTTWLLGSSLR*ELVRNQQSVAKTSPANKKWTNTTEMIVKAAWG 347
Query: 381 ALSPYAAQ*ADVANTRKRIRITTNAGSSRAHWFFVCL*YLVC**TTFGEVQR*TICQNVY 202
ALSP AAQ*ADVANTRKRIR+T NAGSSRAHWFFVCL*YLVC *TTFGEVQR*TICQNVY
Sbjct: 346 ALSPNAAQ*ADVANTRKRIRMTINAGSSRAHWFFVCL*YLVCW*TTFGEVQR*TICQNVY 167
Query: 201 SISLCCHLTM 172
SIS+CCHLT+
Sbjct: 166 SISICCHLTI 137
Score = 246 bits (531), Expect = 2e-064
Identities = 103/148 (69%), Positives = 122/148 (82%)
Frame = -2 / -2
Query: 562 ERVTMNVKCIEPLHALLNNMAVRFLSQVRICKEPAKCGQNYPSKQEMEKHNRSDC*SSM* 383
ERV +N+KCI+PL L ++MA+RFL Q+RI K+PAKCGQN PSKQEM+KHNR DC*SSM
Sbjct: 527 ERVAVNIKCIQPLDTLFDHMAIRFLPQIRIGKKPAKCGQN*PSKQEMDKHNRDDC*SSMG 348
Query: 382 GTLSICCTVS*CCKHEEKNQNNNKCRIITGPLVLCLLVIFGVLIDYFW*STEMNHLPKCV 203
TLS CTVS*C KH+EKN N+NKCRII PLVLCLLVIFGVL+DYFW*ST+MNHLPK V
Sbjct: 347 RTLSKRCTVS*CRKHKEKN*NDNKCRIIPSPLVLCLLVIFGVLVDYFW*STKMNHLPKRV 168
Query: 202 FNIPLLPLDDAAEVKKKTPPGRSGRPRA 119
F+I LLPLD+ AE++ + G +G P A
Sbjct: 167 FDIHLLPLDNTAEIRTEERRGGAGGPPA 84
Score = 229 bits (494), Expect = 3e-059
Identities = 97/132 (73%), Positives = 105/132 (79%)
Frame = +1 / +2
Query: 166 PPHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPRVC 345
PP+ QVA GY IH+LANGSSLYFTKSSL AHQISQANKEPVG *SCI C+S+SFP VC
Sbjct: 131 PPYCQVAANGYRIHVLANGSSLYFTKSSLPAHQISQANKEPVGSG*SCIYCHSNSFPCVC 310
Query: 346 NISLLCSIWRECLTCCSNNHFGCVSPFLVCWDSFGHTLLVPYKFLLERGT*QPCC*ATRG 525
+ISLLCS+WREC CC NNH GCV PFLVCW SFGHTLLV Y+FL E GT* PC
Sbjct: 311 DISLLCSVWRECAPCCFNNHLGCVCPFLVCWASFGHTLLVSYQFLSEGGT**PCGRTACR 490
Query: 526 VALCI*RSL*LV 561
VA+CI* SL LV
Sbjct: 491 VAVCI*CSLQLV 526
Score = 204 bits (441), Expect = 6e-052
Identities = 90/149 (60%), Positives = 101/149 (67%)
Frame = -1 / -1
Query: 563 RTSYNERQMHRATPRVAQQHGC*VPLSSKNL*GTSKVWPKLSQQTRNGETQPK*LLEQHV 384
RTS +E QMH AT + HG *VP S KN * TSKVWPKL+QQTRNG+TQP+*LL+QH
Sbjct: 528 RTSCSEHQMHTATRHAVRPHGY*VPPSDKNW*ETSKVWPKLAQQTRNGQTQPR*LLKQHG 349
Query: 383 RXXXXXXXXXXXLQTRGKESE*QQMQDHHGPTGSLFACDIWCADRLLLVKYRDEPFAKMC 204
QT+GKE E*Q MQDH PTGSLFACDIWCA RLLLVKY+DEPFAK C
Sbjct: 348 AHSLQTLHSKLMSQTQGKELE*Q*MQDHPEPTGSLFACDIWCAGRLLLVKYKDEPFAKTC 169
Query: 203 IQYPFVAT*RCGGGKKKNTPRAEREAPRP 117
I+YPF AT*+ GG + P P
Sbjct: 168 IRYPFAAT*QYGGDTDRRAAGRSGRPPGP 82
Score = 102 bits (218), Expect(2) = 5e-040
Identities = 48/69 (69%), Positives = 51/69 (73%)
Frame = +3 / +1
Query: 357 TVQHMERVPHMLL*QSLRLCFSISCLLG*FWPHFAGSLQILT*ERNLTAMLLSNAWSGSM 536
TVQ +ERV MLL*QS RLC SISCLLG*FWPHFAG L IL * RNL AM ++ SG M
Sbjct: 322 TVQRLERVRPMLL*QSSRLCLSISCLLG*FWPHFAGFLPILI*GRNLIAMWSNSVSSGCM 501
Query: 537 HLTFIVTRS 563
HL F TRS
Sbjct: 502 HLMFTATRS 528
Score = 82.6 bits (174), Expect(2) = 5e-040
Identities = 41/62 (66%), Positives = 42/62 (67%)
Frame = +3 / +1
Query: 120 ARGLPLRPGGVFFFTSAASSSGNKGILNTHFGKWFISVLHQK*SISTPNITSKQRTSGPV 299
A G P P SA SSG+K I NT FGKWFI VLHQK*S STPNITSKQRTSG
Sbjct: 85 AGGPPAPPRRSSVRISAVLSSGSKWISNTRFGKWFIFVLHQK*STSTPNITSKQRTSGLG 264
Query: 300 MI 305
MI
Sbjct: 265 MI 270
Score = 38.2 bits (77), Expect(2) = 1e-092
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +1 / +3
Query: 94 MLPTTASKGRGASRSARGVF 153
MLPTTASKGRGA+RSA +F
Sbjct: 60 MLPTTASKGRGAARSAPPLF 119
Score = 35.9 bits (72), Expect(2) = 1e-074
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = -1 / -3
Query: 140 AEREAPRPLDAVVGSI 93
AER APRPLDAVVGSI
Sbjct: 106 AERAAPRPLDAVVGSI 59
>gb|BI778984.2|BI778984 EBro01_SQ002_G21_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ002_G21 5', mRNA sequence
Length = 526
Score = 300 bits (650), Expect(2) = 6e-086
Identities = 119/126 (94%), Positives = 123/126 (97%)
Frame = +2 / +2
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRIVKWQQ DIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAF+VILILFLV
Sbjct: 149 YLRRIVKWQQMDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFIVILILFLV 328
Query: 341 FATSAYCAAYGESASHAALTITSVVFLHFLFAGIVLATLCWFLTNSYLREEPNSHVVEQR 520
FATSAYCAA+GESA HAALTI SVVF+HFLFAG+VLATLCWFLTNSYLREEPNSHVVEQR
Sbjct: 329 FATSAYCAAFGESAPHAALTIISVVFVHFLFAGLVLATLCWFLTNSYLREEPNSHVVEQR 508
Query: 521 VEWLYA 538
VEWLYA
Sbjct: 509 VEWLYA 526
Score = 248 bits (536), Expect(2) = 1e-069
Identities = 106/120 (88%), Positives = 112/120 (93%)
Frame = -3 / -2
Query: 531 SHSTRCSTTWLLGSSLK*EFVRNQQSVAKTIPANKKWRNTTEVIVRAACEALSPYAAQ*A 352
SHSTRCSTTWLLGSSL+*E VRNQQSVAKT PANKKW NTTE+IV+AA ALSP AAQ*A
Sbjct: 519 SHSTRCSTTWLLGSSLR*ELVRNQQSVAKTSPANKKWTNTTEMIVKAAWGALSPNAAQ*A 340
Query: 351 DVANTRKRIRITTNAGSSRAHWFFVCL*YLVC**TTFGEVQR*TICQNVYSISLCCHLTM 172
DVANTRKRIR+T NAGSSRAHWFFVCL*YLVC *TTFGEVQR*TICQNVYSIS+CCHLT+
Sbjct: 339 DVANTRKRIRMTINAGSSRAHWFFVCL*YLVCW*TTFGEVQR*TICQNVYSISICCHLTI 160
Score = 232 bits (502), Expect = 2e-060
Identities = 98/140 (70%), Positives = 115/140 (82%)
Frame = -2 / -1
Query: 538 CIEPLHALLNNMAVRFLSQVRICKEPAKCGQNYPSKQEMEKHNRSDC*SSM*GTLSICCT 359
CI+PL L ++MA+RFL Q+RI K+PAKCGQN PSKQEM+KHNR DC*SSM TLS CT
Sbjct: 526 CIQPLDTLFDHMAIRFLPQIRIGKKPAKCGQN*PSKQEMDKHNRDDC*SSMGRTLSKRCT 347
Query: 358 VS*CCKHEEKNQNNNKCRIITGPLVLCLLVIFGVLIDYFW*STEMNHLPKCVFNIPLLPL 179
VS*C KH+EKN N+NKCRII PLVLCLLVIFGVL+DYFW*ST+MNHLPK VF+I LLPL
Sbjct: 346 VS*CRKHKEKN*NDNKCRIIPSPLVLCLLVIFGVLVDYFW*STKMNHLPKRVFDIHLLPL 167
Query: 178 DDAAEVKKKTPPGRSGRPRA 119
D+ AE++ + G +G P A
Sbjct: 166 DNTAEIRTEERRGGAGGPPA 107
Score = 222 bits (480), Expect(2) = 6e-058
Identities = 91/124 (73%), Positives = 99/124 (79%)
Frame = +1 / +1
Query: 166 PPHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPRVC 345
PP+ QVA GY IH+LANGSSLYFTKSSL AHQISQANKEPVG *SCI C+S+SFP VC
Sbjct: 154 PPYCQVAANGYRIHVLANGSSLYFTKSSLPAHQISQANKEPVGSG*SCIYCHSNSFPCVC 333
Query: 346 NISLLCSIWRECLTCCSNNHFGCVSPFLVCWDSFGHTLLVPYKFLLERGT*QPCC*ATRG 525
+ISLLCS+WREC CC NNH GCV PFLVCW SFGHTLLV Y+FL E GT* PC
Sbjct: 334 DISLLCSVWRECAPCCFNNHLGCVCPFLVCWASFGHTLLVSYQFLSEGGT**PCGRTACR 513
Query: 526 VALC 537
VA+C
Sbjct: 514 VAVC 525
Score = 192 bits (413), Expect = 4e-048
Identities = 84/140 (60%), Positives = 94/140 (67%)
Frame = -1 / -3
Query: 536 HRATPRVAQQHGC*VPLSSKNL*GTSKVWPKLSQQTRNGETQPK*LLEQHVRXXXXXXXX 357
H AT + HG *VP S KN * TSKVWPKL+QQTRNG+TQP+*LL+QH
Sbjct: 524 HTATRHAVRPHGY*VPPSDKNW*ETSKVWPKLAQQTRNGQTQPR*LLKQHGAHSLQTLHS 345
Query: 356 XXXLQTRGKESE*QQMQDHHGPTGSLFACDIWCADRLLLVKYRDEPFAKMCIQYPFVAT* 177
QT+GKE E*Q MQDH PTGSLFACDIWCA RLLLVKY+DEPFAK CI+YPF AT*
Sbjct: 344 KLMSQTQGKELE*Q*MQDHPEPTGSLFACDIWCAGRLLLVKYKDEPFAKTCIRYPFAAT* 165
Query: 176 RCGGGKKKNTPRAEREAPRP 117
+ GG + P P
Sbjct: 164 QYGGDTDRRAAGRSGRPPGP 105
Score = 89.0 bits (188), Expect(2) = 6e-036
Identities = 42/60 (70%), Positives = 45/60 (75%)
Frame = +3 / +3
Query: 357 TVQHMERVPHMLL*QSLRLCFSISCLLG*FWPHFAGSLQILT*ERNLTAMLLSNAWSGSM 536
TVQ +ERV MLL*QS RLC SISCLLG*FWPHFAG L IL * RNL AM ++ SG M
Sbjct: 345 TVQRLERVRPMLL*QSSRLCLSISCLLG*FWPHFAGFLPILI*GRNLIAMWSNSVSSGCM 524
Score = 82.6 bits (174), Expect(2) = 6e-036
Identities = 41/62 (66%), Positives = 42/62 (67%)
Frame = +3 / +3
Query: 120 ARGLPLRPGGVFFFTSAASSSGNKGILNTHFGKWFISVLHQK*SISTPNITSKQRTSGPV 299
A G P P SA SSG+K I NT FGKWFI VLHQK*S STPNITSKQRTSG
Sbjct: 108 AGGPPAPPRRSSVRISAVLSSGSKWISNTRFGKWFIFVLHQK*STSTPNITSKQRTSGLG 287
Query: 300 MI 305
MI
Sbjct: 288 MI 293
Score = 38.2 bits (77), Expect(2) = 6e-086
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +1 / +2
Query: 94 MLPTTASKGRGASRSARGVF 153
MLPTTASKGRGA+RSA +F
Sbjct: 83 MLPTTASKGRGAARSAPPLF 142
Score = 35.9 bits (72), Expect(2) = 1e-069
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = -1 / -2
Query: 140 AEREAPRPLDAVVGSI 93
AER APRPLDAVVGSI
Sbjct: 129 AERAAPRPLDAVVGSI 82
Score = 22.6 bits (43), Expect(2) = 6e-058
Identities = 11/41 (26%), Positives = 16/41 (39%)
Frame = +3 / +1
Query: 30 AGASAIPEQTRGVXXXXXXXXXXXXXRVQGARGLPLRPGGV 152
A ++A ++ G VQG G PLRP +
Sbjct: 19 AASAAADRESGGAAEGRRGENDAAHDGVQGPGGRPLRPAAL 141
>gb|BE454747.1|BE454747 HVSMEh0095M02f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0095M02f, mRNA sequence
Length = 912
Score = 269 bits (582), Expect(2) = 7e-077
Identities = 112/136 (82%), Positives = 118/136 (86%)
Frame = +2 / -2
Query: 425 FLFAGIVLATLCWFLTNSYLREEPNSHVVEQRVEWLYAFDVHCNSFFPAFVILYVVQYFL 604
F G V L FL N YL + NSHV +QRVEWLYAF VHCNSFF AFVILYV+QYFL
Sbjct: 710 FWLVGQVWPPLACFLPNFYLGRDLNSHVFDQRVEWLYAFIVHCNSFFXAFVILYVLQYFL 531
Query: 605 SPLLVAHGFFPALLSNLLFVVAISYYHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLM 784
SPLL+AHGF PALLSNLLF+VAISYYHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLM
Sbjct: 530 SPLLIAHGFLPALLSNLLFMVAISYYHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLM 351
Query: 785 ILIGFNPTRYFLSLYF 832
IL+GFNPTRYFLSLYF
Sbjct: 350 ILVGFNPTRYFLSLYF 303
Score = 221 bits (477), Expect(2) = 2e-060
Identities = 99/143 (69%), Positives = 110/143 (76%)
Frame = -3 / +1
Query: 831 KYKLRKYLVGLNPISIISGDRMMTRPIGYRKNVVLSRNGRTSYPRKFR****EIATTKSR 652
KYKLRKY VGLNP SI+SGDRMMT PIGY+KNVV S+NGRTSYPRKFR****EIAT R
Sbjct: 304 KYKLRKYRVGLNPTSIMSGDRMMTSPIGYKKNVVRSKNGRTSYPRKFR****EIATMNRR 483
Query: 651 LDNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA*SHSTRCSTTWLLGSSLK*EF 472
DN+AGK PCA NRGD+KY +TYRMTNA KNELQ*T NA SHSTR S TWLL S K*+
Sbjct: 484 FDNRAGKNPCAINRGDKKY*STYRMTNAXKNELQ*TINAYSHSTRWSNTWLLRSLPK*KL 663
Query: 471 VRNQQSVAKTIPANKKWRNTTEV 403
+ +T P N+K N T +
Sbjct: 664 GKKHARGGQTCPTNQKMTNHTPI 732
Score = 157 bits (337), Expect = 1e-037
Identities = 72/149 (48%), Positives = 99/149 (66%)
Frame = -2 / +3
Query: 862 YMAF*P*TLTEVQA*KVSCRIKPY*YHKWRQDDDETNRIQEECCXXXXXXXXXXXKVQVV 683
Y+ +* L EVQA KVSC IKPY YH+WR +DD++NR+QE+CC +VQ+V
Sbjct: 273 YLTY*LPLLPEVQAQKVSCWIKPYEYHEWR*NDDKSNRVQEKCCSVQKWKNIIS*EVQIV 452
Query: 682 IIRNCHHEE*IR*QSWKEAMRDQ*RRQKILHHI*DDKRRKERVTMNVKCIEPLHALLNNM 503
IIRNCHHE+ + * +E+M DQ RRQK+L HI*DD+ +ERV MN KCI+PL L+ +M
Sbjct: 453 IIRNCHHEQEV***GRQESMCDQQRRQKVLKHI*DDECXEERVAMNNKCIQPLDTLVEHM 632
Query: 502 AVRFLSQVRICKEPAKCGQNYPSKQEMEK 416
A++ ++I E K N P + E ++
Sbjct: 633 AIKVPP*IKIG*ETRKRWPNLPHQPENDQ 719
Score = 129 bits (276), Expect = 3e-029
Identities = 75/157 (47%), Positives = 92/157 (58%)
Frame = -1 / +2
Query: 869 LLVHGILAVDFNRSTSLESILSD*TLLVS*VETG**RDQSDTGRMLFYLEMEEHHILESS 690
LL+ +L STS ESI+ D*TL VS*V **+ QS T +MLF +MEEHHIL SS
Sbjct: 266 LLLLNVLVTTLTGSTSSESIVLD*TLRVS*VAIE**QVQSGTRKMLFGPKMEEHHILGSS 445
Query: 689 GSDNKKLPPRRVD*ITKLERSHARPIEATENTAPHIG*QTQERTSYNERQMHRATPRVAQ 510
SDNKKLPP I R H R EAT++ HIG*+ RTS NE+ MH AT +
Sbjct: 446 DSDNKKLPP*TGGLIIGQARIHVRSTEATKSIEAHIG*RMXGRTSCNEQ*MHTATRHAGR 625
Query: 509 QHGC*VPLSSKNL*GTSKVWPKLSQQTRNGETQPK*L 399
HG * P +KN ++ KL+ TR T P+*+
Sbjct: 626 THGY*GPSLNKNWVRNTQEVAKLAPPTRK*PTTPR*I 736
Score = 85.4 bits (180), Expect(3) = 1e-027
Identities = 43/96 (44%), Positives = 55/96 (57%)
Frame = +1 / -1
Query: 409 GCVSPFLVCWDSFGHTLLVPYKFLLERGT*QPCC*ATRGVALCI*RSL*LVLSCVCHPIC 588
G V FLV S + + +FL G * PC VA+CI SL LVL C+ HPIC
Sbjct: 726 GVVGHFLVGGASLATSCVFLTQFLFREGP**PCVRPACRVAVCIYCSLQLVLPCIRHPIC 547
Query: 589 GAVFSVASIGRAWLLSSFVI*STLRGGNFLLSLPEL 696
++ VAS+ R W+L+ +I + GGNFLLSL EL
Sbjct: 546 ASILFVASVDRTWILACPIIKPPVHGGNFLLSLSEL 439
Score = 46.4 bits (95), Expect(3) = 1e-027
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +1 / -1
Query: 745 VSDWSRHHPVSTYDTNRV*SDKILSKLVLRLK 840
V DW+ HH ++T+DT RV*S+ ILS+LVL ++
Sbjct: 390 VPDWTCHHSIATHDTRRV*SNTILSELVLPVR 295
Score = 39.1 bits (79), Expect(2) = 7e-077
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +1 / -1
Query: 910 HTL**SILDILCERGRVKFSKARSI 984
HT+* SILD+LC RGR F KA+SI
Sbjct: 234 HTV*NSILDLLCYRGRSSFRKAQSI 160
Score = 32.2 bits (64), Expect(2) = 2e-060
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = -2 / +3
Query: 982 CSELY*TSLAPVHTRYLKCFTTEYV 908
CSEL+*+S+ P TR L+C+ YV
Sbjct: 162 CSELF*SSIFPDSTRDLRCYFKLYV 236
Score = 29.9 bits (59), Expect(3) = 1e-027
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +3 / -3
Query: 903 LDTYSVVKHFRYLV*TGASEVQ*SSEH 983
+ TYS+ H R LV +G E+Q SSEH
Sbjct: 241 IGTYSLK*HLRSLVLSGKIELQKSSEH 161
>gb|AV922181.1|AV922181 AV922181 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19p08 3', mRNA sequence
Length = 620
Score = 264 bits (572), Expect(2) = 2e-075
Identities = 106/111 (95%), Positives = 110/111 (99%)
Frame = +2 / -1
Query: 500 SHVVEQRVEWLYAFDVHCNSFFPAFVILYVVQYFLSPLLVAHGFFPALLSNLLFVVAISY 679
SHVVEQRVEWLYAFDVHCNSFFPAFVILYV+QYFLSPLL+AHGF PALLSNLLF+VAISY
Sbjct: 620 SHVVEQRVEWLYAFDVHCNSFFPAFVILYVLQYFLSPLLIAHGFLPALLSNLLFMVAISY 441
Query: 680 YHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLMILIGFNPTRYFLSLYF 832
YHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLMIL+GFNPTRYFLSLYF
Sbjct: 440 YHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLMILVGFNPTRYFLSLYF 288
Score = 216 bits (467), Expect(2) = 5e-059
Identities = 92/110 (83%), Positives = 98/110 (89%)
Frame = -3 / +1
Query: 831 KYKLRKYLVGLNPISIISGDRMMTRPIGYRKNVVLSRNGRTSYPRKFR****EIATTKSR 652
KYKLRKY VGLNP SI+SGDRMMT PIGY+KNVV S+NGRTSYPRKFR****EIAT R
Sbjct: 289 KYKLRKYRVGLNPTSIMSGDRMMTSPIGYKKNVVRSKNGRTSYPRKFR****EIATMNRR 468
Query: 651 LDNKAGKKPCATNRGDRKYCTTYRMTNAGKNELQ*TSNA*SHSTRCSTTW 502
DN+AGK PCA NRGD+KY +TYRMTNAGKNELQ*TSNA SHSTRCSTTW
Sbjct: 469 FDNRAGKNPCAINRGDKKY*STYRMTNAGKNELQ*TSNAYSHSTRCSTTW 618
Score = 153 bits (328), Expect = 2e-036
Identities = 66/121 (54%), Positives = 88/121 (72%)
Frame = -2 / +3
Query: 862 YMAF*P*TLTEVQA*KVSCRIKPY*YHKWRQDDDETNRIQEECCXXXXXXXXXXXKVQVV 683
Y+ +* L EVQA KVSC IKPY YH+WR +DD++NR+QE+CC +VQ+V
Sbjct: 258 YLTY*LPLLPEVQAQKVSCWIKPYEYHEWR*NDDKSNRVQEKCCSVQKWKNIIS*EVQIV 437
Query: 682 IIRNCHHEE*IR*QSWKEAMRDQ*RRQKILHHI*DDKRRKERVTMNVKCIEPLHALLNNM 503
IIRNCHHE+ + * +E+M DQ RRQK+L HI*DD+ R+ERV +N+KCI+PL L ++M
Sbjct: 438 IIRNCHHEQEV***GRQESMCDQQRRQKVLKHI*DDECREERVAVNIKCIQPLDTLFDHM 617
Query: 502 A 500
A
Sbjct: 618 A 620
Score = 120 bits (257), Expect = 1e-026
Identities = 65/123 (52%), Positives = 76/123 (61%)
Frame = -1 / +2
Query: 869 LLVHGILAVDFNRSTSLESILSD*TLLVS*VETG**RDQSDTGRMLFYLEMEEHHILESS 690
LL+ +L STS ESI+ D*TL VS*V **+ QS T +MLF +MEEHHIL SS
Sbjct: 251 LLLLNVLVTTLTGSTSSESIVLD*TLRVS*VAIE**QVQSGTRKMLFGPKMEEHHILGSS 430
Query: 689 GSDNKKLPPRRVD*ITKLERSHARPIEATENTAPHIG*QTQERTSYNERQMHRATPRVAQ 510
SDNKKLPP I R H R EAT++ HIG*+ Q RTS +E QMH AT +
Sbjct: 431 DSDNKKLPP*TGGLIIGQARIHVRSTEATKSIEAHIG*RMQGRTSCSEHQMHTATRHAVR 610
Query: 509 QHG 501
HG
Sbjct: 611 PHG 619
Score = 76.2 bits (160), Expect(3) = 5e-025
Identities = 34/65 (52%), Positives = 43/65 (66%)
Frame = +1 / -3
Query: 502 PCC*ATRGVALCI*RSL*LVLSCVCHPICGAVFSVASIGRAWLLSSFVI*STLRGGNFLL 681
PC VA+CI* SL LVL C+ HPIC ++ VAS+ R W+L+ +I + GGNFLL
Sbjct: 618 PCGRTACRVAVCI*CSLQLVLPCIRHPICASILFVASVDRTWILACPIIKPPVHGGNFLL 439
Query: 682 SLPEL 696
SL EL
Sbjct: 438 SLSEL 424
Score = 46.4 bits (95), Expect(3) = 5e-025
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +1 / -3
Query: 745 VSDWSRHHPVSTYDTNRV*SDKILSKLVLRLK 840
V DW+ HH ++T+DT RV*S+ ILS+LVL ++
Sbjct: 375 VPDWTCHHSIATHDTRRV*SNTILSELVLPVR 280
Score = 39.1 bits (79), Expect(2) = 2e-075
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +1 / -3
Query: 910 HTL**SILDILCERGRVKFSKARSI 984
HT+* SILD+LC RGR F KA+SI
Sbjct: 219 HTV*NSILDLLCYRGRSSFRKAQSI 145
Score = 32.2 bits (64), Expect(2) = 5e-059
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = -2 / +3
Query: 982 CSELY*TSLAPVHTRYLKCFTTEYV 908
CSEL+*+S+ P TR L+C+ YV
Sbjct: 147 CSELF*SSIFPDSTRDLRCYFKLYV 221
Score = 29.9 bits (59), Expect(3) = 5e-025
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +3 / -2
Query: 903 LDTYSVVKHFRYLV*TGASEVQ*SSEH 983
+ TYS+ H R LV +G E+Q SSEH
Sbjct: 226 IGTYSLK*HLRSLVLSGKIELQKSSEH 146
>gb|BU998909.1|BU998909 HI12I16r HI Hordeum vulgare subsp. vulgare cDNA clone HI12I16
5-PRIME, mRNA sequence
Length = 534
Score = 265 bits (574), Expect(2) = 2e-075
Identities = 105/112 (93%), Positives = 109/112 (97%)
Frame = +2 / +1
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRIVKWQQ DIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAF+VILILFLV
Sbjct: 199 YLRRIVKWQQMDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFIVILILFLV 378
Query: 341 FATSAYCAAYGESASHAALTITSVVFLHFLFAGIVLATLCWFLTNSYLREEP 496
FATSAYCAA+GESA HAALTI SVVF+HFLFAG+VLATLCWFLTNSYLREEP
Sbjct: 379 FATSAYCAAFGESAPHAALTIISVVFVHFLFAGLVLATLCWFLTNSYLREEP 534
Score = 217 bits (468), Expect(2) = 3e-060
Identities = 94/108 (87%), Positives = 100/108 (92%)
Frame = -3 / -2
Query: 495 GSSLK*EFVRNQQSVAKTIPANKKWRNTTEVIVRAACEALSPYAAQ*ADVANTRKRIRIT 316
GSSL+*E VRNQQSVAKT PANKKW NTTE+IV+AA ALSP AAQ*ADVANTRKRIR+T
Sbjct: 533 GSSLR*ELVRNQQSVAKTSPANKKWTNTTEMIVKAAWGALSPNAAQ*ADVANTRKRIRMT 354
Query: 315 TNAGSSRAHWFFVCL*YLVC**TTFGEVQR*TICQNVYSISLCCHLTM 172
NAGSSRAHWFFVCL*YLVC *TTFGEVQR*TICQNVYSIS+CCHLT+
Sbjct: 353 INAGSSRAHWFFVCL*YLVCW*TTFGEVQR*TICQNVYSISICCHLTI 210
Score = 214 bits (461), Expect(2) = 2e-055
Identities = 85/110 (77%), Positives = 92/110 (83%)
Frame = +1 / +3
Query: 166 PPHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPRVC 345
PP+ QVA GY IH+LANGSSLYFTKSSL AHQISQANKEPVG *SCI C+S+SFP VC
Sbjct: 204 PPYCQVAANGYRIHVLANGSSLYFTKSSLPAHQISQANKEPVGSG*SCIYCHSNSFPCVC 383
Query: 346 NISLLCSIWRECLTCCSNNHFGCVSPFLVCWDSFGHTLLVPYKFLLERGT 495
+ISLLCS+WREC CC NNH GCV PFLVCW SFGHTLLV Y+FL E GT
Sbjct: 384 DISLLCSVWRECAPCCFNNHLGCVCPFLVCWASFGHTLLVSYQFLSEGGT 533
Score = 213 bits (459), Expect = 2e-054
Identities = 91/126 (72%), Positives = 104/126 (82%)
Frame = -2 / -1
Query: 496 RFLSQVRICKEPAKCGQNYPSKQEMEKHNRSDC*SSM*GTLSICCTVS*CCKHEEKNQNN 317
RFL Q+RI K+PAKCGQN PSKQEM+KHNR DC*SSM TLS CTVS*C KH+EKN N+
Sbjct: 534 RFLPQIRIGKKPAKCGQN*PSKQEMDKHNRDDC*SSMGRTLSKRCTVS*CRKHKEKN*ND 355
Query: 316 NKCRIITGPLVLCLLVIFGVLIDYFW*STEMNHLPKCVFNIPLLPLDDAAEVKKKTPPGR 137
NKCRII PLVLCLLVIFGVL+DYFW*ST+MNHLPK VF+I LLPLD+ AE++ + G
Sbjct: 354 NKCRIIPSPLVLCLLVIFGVLVDYFW*STKMNHLPKRVFDIHLLPLDNTAEIRTEERRGG 175
Query: 136 SGRPRA 119
+G P A
Sbjct: 174 AGGPPA 157
Score = 179 bits (386), Expect = 2e-044
Identities = 78/126 (61%), Positives = 87/126 (69%)
Frame = -1 / -3
Query: 494 VPLSSKNL*GTSKVWPKLSQQTRNGETQPK*LLEQHVRXXXXXXXXXXXLQTRGKESE*Q 315
VP S KN * TSKVWPKL+QQTRNG+TQP+*LL+QH QT+GKE E*Q
Sbjct: 532 VPPSDKNW*ETSKVWPKLAQQTRNGQTQPR*LLKQHGAHSLQTLHSKLMSQTQGKELE*Q 353
Query: 314 QMQDHHGPTGSLFACDIWCADRLLLVKYRDEPFAKMCIQYPFVAT*RCGGGKKKNTPRAE 135
MQDH PTGSLFACDIWCA RLLLVKY+DEPFAK CI+YPF AT*+ GG +
Sbjct: 352 *MQDHPEPTGSLFACDIWCAGRLLLVKYKDEPFAKTCIRYPFAAT*QYGGDTDRRAAGRS 173
Query: 134 REAPRP 117
P P
Sbjct: 172 GRPPGP 155
Score = 82.6 bits (174), Expect(2) = 4e-033
Identities = 41/62 (66%), Positives = 42/62 (67%)
Frame = +3 / +2
Query: 120 ARGLPLRPGGVFFFTSAASSSGNKGILNTHFGKWFISVLHQK*SISTPNITSKQRTSGPV 299
A G P P SA SSG+K I NT FGKWFI VLHQK*S STPNITSKQRTSG
Sbjct: 158 AGGPPAPPRRSSVRISAVLSSGSKWISNTRFGKWFIFVLHQK*STSTPNITSKQRTSGLG 337
Query: 300 MI 305
MI
Sbjct: 338 MI 343
Score = 79.4 bits (167), Expect(2) = 4e-033
Identities = 36/46 (78%), Positives = 37/46 (80%)
Frame = +3 / +2
Query: 357 TVQHMERVPHMLL*QSLRLCFSISCLLG*FWPHFAGSLQILT*ERN 494
TVQ +ERV MLL*QS RLC SISCLLG*FWPHFAG L IL * RN
Sbjct: 395 TVQRLERVRPMLL*QSSRLCLSISCLLG*FWPHFAGFLPILI*GRN 532
Score = 38.2 bits (77), Expect(2) = 2e-075
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +1 / +1
Query: 94 MLPTTASKGRGASRSARGVF 153
MLPTTASKGRGA+RSA +F
Sbjct: 133 MLPTTASKGRGAARSAPPLF 192
Score = 35.9 bits (72), Expect(2) = 3e-060
Identities = 15/16 (93%), Positives = 15/16 (93%)
Frame = -1 / -2
Query: 140 AEREAPRPLDAVVGSI 93
AER APRPLDAVVGSI
Sbjct: 179 AERAAPRPLDAVVGSI 132
Score = 22.6 bits (43), Expect(2) = 2e-055
Identities = 11/41 (26%), Positives = 16/41 (39%)
Frame = +3 / +3
Query: 30 AGASAIPEQTRGVXXXXXXXXXXXXXRVQGARGLPLRPGGV 152
A ++A ++ G VQG G PLRP +
Sbjct: 69 AASAAADRESGGAAEGRRGENDAAHDGVQGPGGRPLRPAAL 191
>gb|BI956222.1|BI956222 HVSMEn0001E03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0001E03f, mRNA sequence
Length = 1798
Score = 185 bits (399), Expect(2) = 1e-050
Identities = 76/85 (89%), Positives = 78/85 (91%)
Frame = +2 / +1
Query: 161 YLRRIVKWQQRDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQWARDDPAFVVILILFLV 340
YLRRIVKWQQ DIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQ ARDDPA +VILILFLV
Sbjct: 178 YLRRIVKWQQMDIEYTFWQMVHLCTSPKVVYQHTKYHKQTKNQXARDDPAXIVILILFLV 357
Query: 341 FATSAYCAAYGESASHAALTITSVV 415
ATSAYCAA+GESA HAA TI SVV
Sbjct: 358 CATSAYCAAFGESAPHAAXTIISVV 432
Score = 163 bits (350), Expect = 2e-039
Identities = 65/89 (73%), Positives = 72/89 (80%)
Frame = +1 / +3
Query: 166 PPHRQVATKGY*IHILANGSSLYFTKSSLSAHQISQANKEPVGP**SCICCYSDSFPRVC 345
PP+ QVA GY IH+LANGSSLYFTKSSL AHQISQANKEPV *SC C+S+SFP VC
Sbjct: 183 PPYCQVAANGYRIHVLANGSSLYFTKSSLPAHQISQANKEPVXSG*SCXYCHSNSFPCVC 362
Query: 346 NISLLCSIWRECLTCCSNNHFGCVSPFLV 432
+ISLLCS+WREC CC NNH GCV FL+
Sbjct: 363 DISLLCSVWRECAPCCXNNHLGCVFHFLL 449
Score = 159 bits (341), Expect(2) = 1e-041
Identities = 69/86 (80%), Positives = 77/86 (89%)
Frame = -3 / -3
Query: 429 KKWRNTTEVIVRAACEALSPYAAQ*ADVANTRKRIRITTNAGSSRAHWFFVCL*YLVC** 250
++ +TTE+IV AA ALSP AAQ*ADVA+TRKRIR+T AGSSRAHWFFVCL*YLVC *
Sbjct: 446 QEMEHTTEMIVXAAWGALSPNAAQ*ADVAHTRKRIRMTIXAGSSRAHWFFVCL*YLVCW* 267
Query: 249 TTFGEVQR*TICQNVYSISLCCHLTM 172
TTFGEVQR*TICQNVYSIS+CCHLT+
Sbjct: 266 TTFGEVQR*TICQNVYSISICCHLTI 189
Score = 150 bits (323), Expect = 1e-035
Identities = 66/99 (66%), Positives = 76/99 (76%)
Frame = -2 / -2
Query: 415 HNRSDC*SSM*GTLSICCTVS*CCKHEEKNQNNNKCRIITGPLVLCLLVIFGVLIDYFW* 236
HNR DC SSM TLS CTVS*C H+EKN N+N CRII LVLCLLVIFGVL+DYFW*
Sbjct: 432 HNRDDCXSSMGRTLSKRCTVS*CRTHKEKN*NDNXCRIIPSXLVLCLLVIFGVLVDYFW* 253
Query: 235 STEMNHLPKCVFNIPLLPLDDAAEVKKKTPPGRSGRPRA 119
ST+MNHLPK VF+I LLPLD+ AE++ + G +G P A
Sbjct: 252 STKMNHLPKRVFDIHLLPLDNTAEIRTEERRGGAGGPPA 136
Score = 126 bits (269), Expect(2) = 9e-031
Identities = 54/84 (64%), Positives = 59/84 (70%)
Frame = -1 / -1
Query: 416 TQPK*LLEQHVRXXXXXXXXXXXLQTRGKESE*QQMQDHHGPTGSLFACDIWCADRLLLV 237
TQP+*LL QH T+GKE E*Q MQDH TGSLFACDIWCA RLLLV
Sbjct: 433 TQPR*LLXQHGAHSLQTLHSKLMSHTQGKELE*QXMQDHPEXTGSLFACDIWCAGRLLLV 254
Query: 236 KYRDEPFAKMCIQYPFVAT*RCGG 165
KY+DEPFAK CI+YPF AT*+ GG
Sbjct: 253 KYKDEPFAKTCIRYPFAAT*QYGG 182
Score = 35.0 bits (70), Expect(2) = 1e-050
Identities = 15/20 (75%), Positives = 17/20 (85%)
Frame = +1 / +1
Query: 94 MLPTTASKGRGASRSARGVF 153
MLPTTASKGR A+RSA +F
Sbjct: 112 MLPTTASKGRXAARSAPPLF 171
Score = 31.8 bits (63), Expect(2) = 1e-041
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = -1 / -3
Query: 140 AEREAPRPLDAVVGSI 93
AER A RPLDAVVGSI
Sbjct: 158 AERAAXRPLDAVVGSI 111
Score = 28.1 bits (55), Expect(2) = 9e-031
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -2 / -1
Query: 142 GRSGRPRAPWT 110
GRSGRP PWT
Sbjct: 160 GRSGRPXGPWT 128
>gb|BE215119.2|BE215119 HV_CEb0005N21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0005N21f, mRNA sequence
Length = 936
Score = 93.2 bits (197), Expect(5) = 8e-046
Identities = 37/39 (94%), Positives = 38/39 (97%)
Frame = +2 / -2
Query: 716 PFLDRTTFFLYPIGLVIILSPLMILIGFNPTRYFLSLYF 832
PFLDR TFFLYPIGLVIILSPLMIL+GFNPTRYFLSLYF
Sbjct: 398 PFLDRATFFLYPIGLVIILSPLMILVGFNPTRYFLSLYF 282
Score = 78.5 bits (165), Expect(5) = 4e-043
Identities = 31/40 (77%), Positives = 35/40 (87%)
Frame = -3 / +1
Query: 831 KYKLRKYLVGLNPISIISGDRMMTRPIGYRKNVVLSRNGR 712
KYKLRKY VGLNP SI+SGDRMMT PIGY+KNV S+NG+
Sbjct: 283 KYKLRKYRVGLNPTSIMSGDRMMTSPIGYKKNVARSKNGK 402
Score = 64.3 bits (134), Expect(4) = 1e-027
Identities = 26/44 (59%), Positives = 34/44 (77%)
Frame = -2 / +3
Query: 862 YMAF*P*TLTEVQA*KVSCRIKPY*YHKWRQDDDETNRIQEECC 731
Y+ +* L EVQA KVS IKPY YH+WR +DD++NR+QE+CC
Sbjct: 252 YLTY*LPLLPEVQAQKVSGWIKPYEYHEWR*NDDKSNRVQEKCC 383
Score = 57.9 bits (120), Expect(5) = 3e-028
Identities = 22/31 (70%), Positives = 24/31 (77%)
Frame = +2 / -1
Query: 659 FVVAISYYHYLNFLGYDVLPFLDRTTFFLYP 751
F+VAISYYHYLNFLGYDVLP + FL P
Sbjct: 456 FMVAISYYHYLNFLGYDVLPIFGPSNIFLVP 364
Score = 52.4 bits (108), Expect(5) = 4e-043
Identities = 22/31 (70%), Positives = 24/31 (77%)
Frame = -3 / +2
Query: 528 HSTRCSTTWLLGSSLK*EFVRNQQSVAKTIP 436
HSTRCSTTWLL SSL+*EFVR QQ + P
Sbjct: 587 HSTRCSTTWLLRSSLR*EFVRTQQMGPQLCP 679
Score = 52.4 bits (108), Expect(5) = 8e-046
Identities = 20/25 (80%), Positives = 23/25 (92%)
Frame = +2 / -2
Query: 554 NSFFPAFVILYVVQYFLSPLLVAHG 628
+SFFPAFVI YV+QYFLSPLL+ HG
Sbjct: 563 HSFFPAFVIQYVLQYFLSPLLIDHG 489
Score = 48.3 bits (99), Expect(5) = 8e-046
Identities = 20/26 (76%), Positives = 22/26 (84%)
Frame = +2 / -1
Query: 455 LCWFLTNSYLREEPNSHVVEQRVEWL 532
+C LTNSYLRE+ NSHVVEQRVE L
Sbjct: 660 ICCVLTNSYLREDLNSHVVEQRVECL 583
Score = 46.4 bits (95), Expect(5) = 3e-028
Identities = 18/32 (56%), Positives = 26/32 (81%)
Frame = +1 / -1
Query: 745 VSDWSRHHPVSTYDTNRV*SDKILSKLVLRLK 840
V DW+ HH ++T+DT RV*S+ ILS+LVL ++
Sbjct: 369 VPDWTCHHSIATHDTRRV*SNPILSELVLPVR 274
Score = 44.1 bits (90), Expect(5) = 4e-043
Identities = 17/24 (70%), Positives = 20/24 (83%)
Frame = -3 / +1
Query: 627 PCATNRGDRKYCTTYRMTNAGKNE 556
P + NRGD+KY +TY MTNAGKNE
Sbjct: 490 PWSINRGDKKY*STYWMTNAGKNE 561
Score = 43.2 bits (88), Expect(4) = 1e-027
Identities = 21/34 (61%), Positives = 26/34 (76%)
Frame = -3 / +2
Query: 750 GYRKNVVLSRNGRTSYPRKFR****EIATTKSRL 649
G RK ++ + GRTSYP+KFR****EIAT K +
Sbjct: 365 GTRKMLLGPKMGRTSYPKKFR****EIATMKQEV 466
Score = 41.8 bits (85), Expect(5) = 4e-043
Identities = 16/18 (88%), Positives = 16/18 (88%)
Frame = -1 / +3
Query: 716 EEHHILESSGSDNKKLPP 663
EEHHIL SS SDNKKLPP
Sbjct: 399 EEHHILRSSDSDNKKLPP 452
Score = 39.1 bits (79), Expect(5) = 8e-046
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = +1 / -1
Query: 910 HTL**SILDILCERGRVKFSKARSI 984
HT+* SILD+LC RGR F KA+SI
Sbjct: 213 HTV*NSILDLLCYRGRSSFRKAQSI 139
Score = 39.1 bits (79), Expect(4) = 1e-027
Identities = 17/27 (62%), Positives = 21/27 (77%)
Frame = -2 / +3
Query: 634 KEAMRDQ*RRQKILHHI*DDKRRKERV 554
K +M DQ RRQK+L HI DD+ R+ERV
Sbjct: 483 KNSMVDQQRRQKVLKHILDDECREERV 563
Score = 39.1 bits (79), Expect(5) = 3e-028
Identities = 13/24 (54%), Positives = 17/24 (70%)
Frame = +1 / -1
Query: 556 LVLSCVCHPICGAVFSVASIGRAW 627
LVL C+ HPIC ++ VAS+ R W
Sbjct: 561 LVLPCIRHPICASILFVASVDRPW 490
Score = 32.2 bits (64), Expect(4) = 1e-027
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = -2 / +1
Query: 535 IEPLHALLNNMAVRFLSQVRICKEPAKCGQNYP 437
++ L L ++MA++ L Q+RICK A P
Sbjct: 580 LQALDTLFDHMAIKVLPQIRICKNTANGATTMP 678
Score = 32.2 bits (64), Expect(5) = 4e-043
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = -2 / +3
Query: 982 CSELY*TSLAPVHTRYLKCFTTEYV 908
CSEL+*+S+ P TR L+C+ YV
Sbjct: 141 CSELF*SSIFPDSTRDLRCYFKLYV 215
Score = 29.9 bits (59), Expect(5) = 3e-028
Identities = 14/27 (51%), Positives = 18/27 (66%)
Frame = +3 / -3
Query: 903 LDTYSVVKHFRYLV*TGASEVQ*SSEH 983
+ TYS+ H R LV +G E+Q SSEH
Sbjct: 220 IGTYSLK*HLRSLVLSGKIELQKSSEH 140
Score = 25.4 bits (49), Expect(5) = 8e-046
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3 / -2
Query: 660 SWWQFLII 683
SWWQFLII
Sbjct: 455 SWWQFLII 432
Score = 24.4 bits (47), Expect(5) = 3e-028
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = +1 / -3
Query: 457 LLVPYKFLLERGT*QPC 507
LL YKFL E G * PC
Sbjct: 658 LLCSYKFLSEGGP**PC 608
>gb|CB860069.1|CB860069 HI12I16w HI Hordeum vulgare subsp. vulgare cDNA clone HI12I16
3-PRIME, mRNA sequence
Length = 495
Score = 153 bits (328), Expect(2) = 8e-042
Identities = 62/66 (93%), Positives = 64/66 (96%)
Frame = +2 / -2
Query: 635 PALLSNLLFVVAISYYHYLNFLGYDVLPFLDRTTFFLYPIGLVIILSPLMILIGFNPTRY 814
PALLSNLLF+VAI YYHYLNFLGYDV PFLDRTTFFLYPIGLVIILSPLMIL+GFNPTRY
Sbjct: 494 PALLSNLLFMVAIFYYHYLNFLGYDVFPFLDRTTFFLYPIGLVIILSPLMILVGFNPTRY 315
Query: 815 FLSLYF 832
FLSLYF
Sbjct: 314 FLSLYF 297
Score = 118 bits (252), Expect(2) = 3e-030
Identities = 50/66 (75%), Positives = 58/66 (87%)
Frame = -3 / +1
Query: 831 KYKLRKYLVGLNPISIISGDRMMTRPIGYRKNVVLSRNGRTSYPRKFR****EIATTKSR 652
KYKL+KY VGLNP SI+SGDRMMT PIGY+KNVV S+NG+TSYPRKF+****+IAT R
Sbjct: 298 KYKLKKYRVGLNPTSIMSGDRMMTSPIGYKKNVVRSKNGKTSYPRKFK****KIATMNRR 477
Query: 651 LDNKAG 634
DN+AG
Sbjct: 478 FDNRAG 495
Score = 38.2 bits (77), Expect(2) = 8e-042
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = +1 / -1
Query: 910 HTL**SILDILCERGRVKFSKARSI 984
HT+* SILD LC RGR F KA+SI
Sbjct: 228 HTV*NSILDFLCYRGRSSFRKAQSI 154
Score = 34.1 bits (68), Expect(2) = 3e-030
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = -2 / +3
Query: 982 CSELY*TSLAPVHTRYLKCFTTEYV 908
CSEL+*+S+ P TR LKC+ YV
Sbjct: 156 CSELF*SSIFPDSTRNLKCYFKLYV 230
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 555,829,260
Number of Sequences: 312970
Number of extensions: 8709064
Number of successful extensions: 51804
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 14543187612
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)