TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950290.2.2
(867 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CB881351.1|CB881351 HM09I17w HM Hordeum vulgare subsp. v... 151 2e-061
gb|AJ462529.1|AJ462529 AJ462529 S00002 Hordeum vulgare subs... 104 1e-045
gb|BM443421.2|BM443421 EBro02_SQ005_B05_R root, 3 week, hyd... 77 2e-039
gb|BI953204.1|BI953204 HVSMEm0011K04f Hordeum vulgare green... 144 6e-034
gb|BM371147.2|BM371147 EBro04_SQ003_K01_R root, 3 week, sal... 91 6e-032
gb|BU995205.1|BU995205 HM09I17r HM Hordeum vulgare subsp. v... 135 4e-031
gb|BG415787.1|BG415787 HVSMEk0007M21f Hordeum vulgare testa... 91 1e-029
gb|BQ134710.1|BQ134710 LP20067/1 Lemma/palea-enriched cDNA ... 84 3e-029
gb|BQ762795.1|BQ762795 EBro02_SQ006_I06_R root, 3 week, hyd... 74 5e-025
>gb|CB881351.1|CB881351 HM09I17w HM Hordeum vulgare subsp. vulgare cDNA clone HM09I17
3-PRIME, mRNA sequence
Length = 612
Score = 151 bits (325), Expect(2) = 2e-061
Identities = 64/71 (90%), Positives = 67/71 (94%)
Frame = -3 / -2
Query: 772 NVTRFMMLAREPIIPRTDKPFKTSIVFSLEEGPGQLFKALAVFALRDINLTKIESRPHKE 593
NVTRFMMLAREPIIPR DKPFKTSIVFSLEEGPGQLFKALAVFALR+INLTKIESRPHK+
Sbjct: 611 NVTRFMMLAREPIIPRVDKPFKTSIVFSLEEGPGQLFKALAVFALREINLTKIESRPHKK 432
Query: 592 RPLRVSDDCSS 560
RP RV+DD S
Sbjct: 431 RPFRVADDTFS 399
Score = 125 bits (268), Expect(2) = 5e-041
Identities = 55/65 (84%), Positives = 60/65 (92%)
Frame = +3 / +1
Query: 579 TRRGLSLCGRLSIFVRLMSLNANTASALKSCPGPSSSEKTMLVLNGLSVRGMMGSRASII 758
TR+GL LCGRLSI VRL+SL+A TASALKSCPGPSSSE TMLVLNGLS RG++GSRASII
Sbjct: 418 TRKGLFLCGRLSILVRLISLSAKTASALKSCPGPSSSENTMLVLNGLSTRGIIGSRASII 597
Query: 759 KRVTL 773
KRVTL
Sbjct: 598 KRVTL 612
Score = 107 bits (229), Expect(2) = 9e-034
Identities = 44/69 (63%), Positives = 52/69 (75%)
Frame = +2 / +3
Query: 566 TVIRYPKRPFLMWTTFNLCEVDVPQCKYSKCLKELSRSFF**KDYACLKWFISARYDGFS 745
++I Y KRPFL+WT F+L EVD PQCK KCL+ELS SFF *K A L+WFI+ RY+ F
Sbjct: 405 SIISYTKRPFLVWTAFDLGEVDFPQCKNCKCLEELSGSFFK*KHNASLEWFINPRYNRFP 584
Query: 746 S*HHKTGDI 772
S HHK GDI
Sbjct: 585 SKHHKAGDI 611
Score = 104 bits (222), Expect(2) = 2e-061
Identities = 46/58 (79%), Positives = 50/58 (86%)
Frame = -3 / -2
Query: 574 DDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDVNEP*IES 401
D S+ +K FDYLFYVDLEASMADPKTQNAL NL+EFATF RVLGSYPTDV+E *I S
Sbjct: 410 DTFSTRVKYFDYLFYVDLEASMADPKTQNALRNLEEFATFSRVLGSYPTDVSEA*IAS 237
Score = 89.5 bits (189), Expect(2) = 2e-027
Identities = 44/67 (65%), Positives = 46/67 (68%)
Frame = -1 / -3
Query: 771 MSPVL*C*LENPSYLALINHLRQA*SFH*KKDLDSSLRHLLYLH*GTSTSQRLKVVHIRK 592
MSP L*C L N YL LINH R A FH KKD D+S RHL +LH*G STS R K VH RK
Sbjct: 610 MSPAL*CLLGNLLYLGLINHSRLALCFHLKKDPDNSSRHLQFLH*GKSTSPRSKAVHTRK 431
Query: 591 GLFGYLM 571
GLF LM
Sbjct: 430 GLFV*LM 410
Score = 62.5 bits (130), Expect(2) = 5e-041
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = +3 / +1
Query: 402 LSI*GSFTSVG*LPRTLKNVANSFRFPRAF*VFGSAIDA 518
L+I S TSVG*LPRTL+ VANS +F RAF*VFGSAIDA
Sbjct: 238 LAIQASLTSVG*LPRTLEKVANSSKFLRAF*VFGSAIDA 354
Score = 56.1 bits (116), Expect(2) = 9e-034
Identities = 28/49 (57%), Positives = 33/49 (67%)
Frame = +1 / +2
Query: 412 KVHSHL*DSFQELLKMLQTPSDFQEHSESLDQPLMLQGQHKKGNQSSLR 558
K+ S L*DS LLK L+TP +F E SE LDQP QG HK G+QS+ R
Sbjct: 248 KLRSRL*DSSLGLLKRLRTPPNF*ERSEFLDQP*TPQGPHKTGSQST*R 394
Score = 52.8 bits (109), Expect(2) = 2e-027
Identities = 31/66 (46%), Positives = 38/66 (57%)
Frame = -1 / -3
Query: 564 PRS*RTLITFFMLTLKHQWLIQRLRMLLEI*RSLQHF*EFLEAILQM*MNLRLKVLQITY 385
P + TL T FM TL+ WLIQ+LR L EI RS Q F E AILQ * L +VL +
Sbjct: 400 PHASSTLTTCFMWTLRRLWLIQKLRTLSEIWRSSQPFQES*GAILQT*AKLE*QVLAADH 221
Query: 384 WISRNR 367
+ N+
Sbjct: 220 MLVINQ 203
>gb|AJ462529.1|AJ462529 AJ462529 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200021A01F1, mRNA sequence
Length = 360
Score = 104 bits (222), Expect(2) = 1e-045
Identities = 46/58 (79%), Positives = 50/58 (86%)
Frame = -3 / +3
Query: 574 DDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDVNEP*IES 401
D S+ +K FDYLFYVDLEASMADPKTQNAL NL+EFATF RVLGSYPTDV+E *I S
Sbjct: 135 DTFSTRVKYFDYLFYVDLEASMADPKTQNALRNLEEFATFSRVLGSYPTDVSEA*IAS 308
Score = 99.1 bits (210), Expect(2) = 1e-045
Identities = 42/48 (87%), Positives = 45/48 (93%)
Frame = -3 / +3
Query: 703 SIVFSLEEGPGQLFKALAVFALRDINLTKIESRPHKERPLRVSDDCSS 560
SIVFSLEEGPGQLFKALAVFALR+INLTKIESRPHK+RP RV+DD S
Sbjct: 3 SIVFSLEEGPGQLFKALAVFALREINLTKIESRPHKKRPFRVADDTFS 146
Score = 82.2 bits (173), Expect(2) = 5e-028
Identities = 35/42 (83%), Positives = 38/42 (90%)
Frame = +3 / -3
Query: 579 TRRGLSLCGRLSIFVRLMSLNANTASALKSCPGPSSSEKTML 704
TR+GL LCGRLSI VRL+SL+A TASALKSCPGPSSSE TML
Sbjct: 127 TRKGLFLCGRLSILVRLISLSAKTASALKSCPGPSSSENTML 2
Score = 72.1 bits (151), Expect(2) = 4e-023
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = +2 / -2
Query: 566 TVIRYPKRPFLMWTTFNLCEVDVPQCKYSKCLKELSRSFF**KDYA 703
++I Y KRPFL+WT F+L EVD PQCK KCL+ELS SFF *K A
Sbjct: 140 SIISYTKRPFLVWTAFDLGEVDFPQCKNCKCLEELSGSFFK*KHNA 3
Score = 62.5 bits (130), Expect(2) = 5e-028
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = +3 / -3
Query: 402 LSI*GSFTSVG*LPRTLKNVANSFRFPRAF*VFGSAIDA 518
L+I S TSVG*LPRTL+ VANS +F RAF*VFGSAIDA
Sbjct: 307 LAIQASLTSVG*LPRTLEKVANSSKFLRAF*VFGSAIDA 191
Score = 56.1 bits (116), Expect(2) = 4e-023
Identities = 28/49 (57%), Positives = 33/49 (67%)
Frame = +1 / -1
Query: 412 KVHSHL*DSFQELLKMLQTPSDFQEHSESLDQPLMLQGQHKKGNQSSLR 558
K+ S L*DS LLK L+TP +F E SE LDQP QG HK G+QS+ R
Sbjct: 297 KLRSRL*DSSLGLLKRLRTPPNF*ERSEFLDQP*TPQGPHKTGSQST*R 151
>gb|BM443421.2|BM443421 EBro02_SQ005_B05_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ005_B05 5', mRNA sequence
Length = 373
Score = 76.7 bits (161), Expect(3) = 2e-039
Identities = 29/53 (54%), Positives = 40/53 (75%)
Frame = -3 / +1
Query: 580 VSDDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDV 422
V D K+F+Y+FYVD +AS+A+P+ QNAL ++EF +FLRVLGSYP D+
Sbjct: 196 VDDANRGTAKHFEYMFYVDFQASLAEPRAQNALAEVQEFTSFLRVLGSYPMDM 354
Score = 64.3 bits (134), Expect(3) = 2e-039
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = -3 / +1
Query: 685 EEGPGQLFKALAVFALRDINLTKIESRPHKERPLRVSDDCS 563
+EG LFK L+ FA RDI LTKIESRPH+ RP+R+ DD +
Sbjct: 88 KEGTSVLFKVLSAFAFRDITLTKIESRPHRHRPIRLVDDAN 210
Score = 60.2 bits (125), Expect(3) = 2e-039
Identities = 23/27 (85%), Positives = 27/27 (100%)
Frame = -3 / +1
Query: 769 VTRFMMLAREPIIPRTDKPFKTSIVFS 689
VTRF+MLAREPI+PRTD+PFKTSIVF+
Sbjct: 1 VTRFVMLAREPIVPRTDRPFKTSIVFA 81
>gb|BI953204.1|BI953204 HVSMEm0011K04f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0011K04f, mRNA sequence
Length = 636
Score = 144 bits (309), Expect = 6e-034
Identities = 61/74 (82%), Positives = 68/74 (91%)
Frame = -3 / +1
Query: 835 SLAAKLYGLDVLAENIQDDKDNVTRFMMLAREPIIPRTDKPFKTSIVFSLEEGPGQLFKA 656
S+AA+LYGLD+LAENIQDD DNVTRFMMLAREPIIPRTDKPFKTSIVFSLEE PG +FK
Sbjct: 415 SVAAQLYGLDILAENIQDDADNVTRFMMLAREPIIPRTDKPFKTSIVFSLEERPGPVFKG 594
Query: 655 LAVFALRDINLTKI 614
L +FAL+ INLTK+
Sbjct: 595 LWMFALKKINLTKM 636
Score = 102 bits (218), Expect = 2e-021
Identities = 52/74 (70%), Positives = 56/74 (75%)
Frame = +3 / -2
Query: 615 IFVRLMSLNANTASALKSCPGPSSSEKTMLVLNGLSVRGMMGSRASIIKRVTLSLSS*IF 794
I VRL+ AN LK+ PG SS E TMLVLNGLSVRG+MGSRASIIKRVTLS SS IF
Sbjct: 635 ILVRLIFFRANIQRPLKTGPGLSSRENTMLVLNGLSVRGIMGSRASIIKRVTLSASSWIF 456
Query: 795 SARTSNPYSLAANE 836
SAR S+P S AA E
Sbjct: 455 SARISSP*S*AATE 414
>gb|BM371147.2|BM371147 EBro04_SQ003_K01_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ003_K01 5', mRNA sequence
Length = 584
Score = 91.3 bits (193), Expect(3) = 6e-032
Identities = 36/49 (73%), Positives = 45/49 (91%)
Frame = -3 / +2
Query: 835 SLAAKLYGLDVLAENIQDDKDNVTRFMMLAREPIIPRTDKPFKTSIVFS 689
S AA+LYG+++LA+ IQDD NVTRF+MLAREPI+PRTD+PFKTSIVF+
Sbjct: 257 SRAAELYGMEILADGIQDDCGNVTRFVMLAREPIVPRTDRPFKTSIVFA 403
Score = 56.5 bits (117), Expect(3) = 6e-032
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = -3 / +2
Query: 685 EEGPGQLFKALAVFALRDINLTKIESRPHKERPL 584
+EG LFK L+ FA RDI LTKIESRPH+ RP+
Sbjct: 410 KEGTSVLFKVLSAFAFRDITLTKIESRPHRHRPI 511
Score = 27.6 bits (54), Expect(3) = 6e-032
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = -3 / +3
Query: 580 VSDDCSSLLKNFDYLFYVDLEA 515
V D K+F+Y+FYVD +A
Sbjct: 519 VDDANRGTAKHFEYMFYVDFQA 584
>gb|BU995205.1|BU995205 HM09I17r HM Hordeum vulgare subsp. vulgare cDNA clone HM09I17
5-PRIME, mRNA sequence
Length = 654
Score = 135 bits (289), Expect = 4e-031
Identities = 58/64 (90%), Positives = 61/64 (95%)
Frame = -3 / +3
Query: 835 SLAAKLYGLDVLAENIQDDKDNVTRFMMLAREPIIPRTDKPFKTSIVFSLEEGPGQLFKA 656
SLAA+LY LD+LAENIQD+K NVTRFMMLAREPIIPR DKPFKTSIVFSLEEGPGQLFKA
Sbjct: 462 SLAAELYELDILAENIQDEKVNVTRFMMLAREPIIPRVDKPFKTSIVFSLEEGPGQLFKA 641
Query: 655 LAVF 644
LAVF
Sbjct: 642 LAVF 653
Score = 111 bits (238), Expect = 4e-024
Identities = 52/63 (82%), Positives = 56/63 (88%)
Frame = +3 / -3
Query: 648 TASALKSCPGPSSSEKTMLVLNGLSVRGMMGSRASIIKRVTLSLSS*IFSARTSNPYSLA 827
TASALKSCPGPSSSE TMLVLNGLS RG++GSRASIIKRVTL+ SS*IFSAR S+ S A
Sbjct: 649 TASALKSCPGPSSSENTMLVLNGLSTRGIIGSRASIIKRVTLTFSS*IFSARISSS*SSA 470
Query: 828 ANE 836
ANE
Sbjct: 469 ANE 461
>gb|BG415787.1|BG415787 HVSMEk0007M21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0007M21f, mRNA sequence
Length = 638
Score = 91.3 bits (193), Expect(2) = 1e-029
Identities = 36/49 (73%), Positives = 45/49 (91%)
Frame = -3 / +1
Query: 835 SLAAKLYGLDVLAENIQDDKDNVTRFMMLAREPIIPRTDKPFKTSIVFS 689
S AA+LYG+++LA+ IQDD NVTRF+MLAREPI+PRTD+PFKTSIVF+
Sbjct: 373 SRAAELYGMEILADGIQDDCGNVTRFVMLAREPIVPRTDRPFKTSIVFA 519
Score = 58.3 bits (121), Expect(2) = 1e-029
Identities = 23/36 (63%), Positives = 28/36 (77%)
Frame = -3 / +1
Query: 685 EEGPGQLFKALAVFALRDINLTKIESRPHKERPLRV 578
+EG LFK L+ FALRDI LTKIES PH+ RP+R+
Sbjct: 526 KEGTSVLFKVLSAFALRDITLTKIESSPHRHRPIRL 633
>gb|BQ134710.1|BQ134710 LP20067/1 Lemma/palea-enriched cDNA library from gelatinous stage
of kernel Hordeum vulgare subsp. vulgare cDNA clone
2-67/1 similar to Putative chorismate mutase/prephenate
dehydratase, mRNA sequence
Length = 286
Score = 84.5 bits (178), Expect(2) = 3e-029
Identities = 32/43 (74%), Positives = 40/43 (93%)
Frame = -3 / -2
Query: 817 YGLDVLAENIQDDKDNVTRFMMLAREPIIPRTDKPFKTSIVFS 689
YG+++LA+ IQDD NVTRF+MLAREPI+PRTD+PFKTSIVF+
Sbjct: 285 YGMEILADGIQDDCGNVTRFVMLAREPIVPRTDRPFKTSIVFA 157
Score = 64.3 bits (134), Expect(2) = 3e-029
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = -3 / -2
Query: 685 EEGPGQLFKALAVFALRDINLTKIESRPHKERPLRVSDDCS 563
+EG LFK L+ FA RDI LTKIESRPH+ RP+R+ DD +
Sbjct: 150 KEGTSVLFKVLSAFAFRDITLTKIESRPHRHRPIRLVDDAN 28
>gb|BQ762795.1|BQ762795 EBro02_SQ006_I06_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ006_I06 5', mRNA sequence
Length = 455
Score = 74.4 bits (156), Expect(2) = 5e-025
Identities = 29/53 (54%), Positives = 39/53 (73%)
Frame = -3 / +3
Query: 580 VSDDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDV 422
V D K+F+Y+FYVD +AS+A+P+ QNAL +EF +FLRVLGSYP D+
Sbjct: 96 VDDANRGTAKHFEYMFYVDFQASLAEPRAQNALAEGQEFTSFLRVLGSYPMDM 254
Score = 60.2 bits (125), Expect(2) = 5e-025
Identities = 23/35 (65%), Positives = 28/35 (80%)
Frame = -3 / +3
Query: 667 LFKALAVFALRDINLTKIESRPHKERPLRVSDDCS 563
LFK L+ FA RDI LTKIESRPH+ RP+R+ DD +
Sbjct: 6 LFKVLSAFAFRDITLTKIESRPHRHRPIRLVDDAN 110
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 355,381,338
Number of Sequences: 312970
Number of extensions: 4630199
Number of successful extensions: 24031
Number of sequences better than 1.0e-020: 9
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 9936482404
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)