TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950290.2.1
(1442 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU995205.1|BU995205 HM09I17r HM Hordeum vulgare subsp. v... 416 e-115
gb|BI953204.1|BI953204 HVSMEm0011K04f Hordeum vulgare green... 410 e-114
gb|BG415787.1|BG415787 HVSMEk0007M21f Hordeum vulgare testa... 171 4e-086
gb|BU985441.1|BU985441 HF07D15r HF Hordeum vulgare subsp. v... 275 3e-073
gb|BM371147.2|BM371147 EBro04_SQ003_K01_R root, 3 week, sal... 130 3e-067
gb|CB881351.1|CB881351 HM09I17w HM Hordeum vulgare subsp. v... 151 4e-061
gb|BF257794.3|BF257794 HVSMEf0013O05f Hordeum vulgare seedl... 188 7e-055
gb|CA027506.1|CA027506 HZ59C11r HZ Hordeum vulgare subsp. v... 194 3e-050
gb|AJ462529.1|AJ462529 AJ462529 S00002 Hordeum vulgare subs... 104 2e-045
gb|BM443421.2|BM443421 EBro02_SQ005_B05_R root, 3 week, hyd... 77 3e-039
gb|BQ463672.1|BQ463672 HG01B07r HG Hordeum vulgare subsp. v... 151 1e-035
gb|CA027787.1|CA027787 HZ60A11r HZ Hordeum vulgare subsp. v... 150 2e-035
gb|BU979399.1|BU979399 HA16A13r HA Hordeum vulgare subsp. v... 121 7e-035
gb|BU997534.1|BU997534 HI08E10r HI Hordeum vulgare subsp. v... 136 3e-031
gb|BQ134710.1|BQ134710 LP20067/1 Lemma/palea-enriched cDNA ... 84 5e-029
gb|BQ762795.1|BQ762795 EBro02_SQ006_I06_R root, 3 week, hyd... 74 8e-025
>gb|BU995205.1|BU995205 HM09I17r HM Hordeum vulgare subsp. vulgare cDNA clone HM09I17
5-PRIME, mRNA sequence
Length = 654
Score = 416 bits (902), Expect = e-115
Identities = 174/211 (82%), Positives = 186/211 (88%)
Frame = +3 / +3
Query: 186 LKVAYQGCXXXXXXXXXXXXXPNCETVPCEHFDTAFQAVQNWVADRAVLPLENSLGGSIH 365
LKVA G PNC+TVPCEHFDTAFQAVQNW+ DRAVLPLEN+LGGSIH
Sbjct: 21 LKVALPGLPWSDSEAAAKKAYPNCQTVPCEHFDTAFQAVQNWIVDRAVLPLENTLGGSIH 200
Query: 366 RNYDLLLRHSLHIVGEVRLAVRHCLLANPGVKIENLKSAMSHPQALAQCEHTLTSLGIEH 545
RNYDLLLRH LHIVGEVRLAVRHCLLAN GVKIENL++ +SHPQALAQCEHTLT LGIEH
Sbjct: 201 RNYDLLLRHELHIVGEVRLAVRHCLLANRGVKIENLRNVISHPQALAQCEHTLTELGIEH 380
Query: 546 REAVDDTAGAAKIVAEHMLQDTGAIASSLAAKLYGLDVLAENIQDDKDNVTRFMMLAREP 725
R+AVDDTAGAAK+VAE MLQDTGA+ASSLAA+LY LD+LAENIQD+K NVTRFMMLAREP
Sbjct: 381 RQAVDDTAGAAKLVAEQMLQDTGAVASSLAAELYELDILAENIQDEKVNVTRFMMLAREP 560
Query: 726 IIPRTDKPFKTSIVFSLEEGPGQLFKALAVF 818
IIPR DKPFKTSIVFSLEEGPGQLFKALAVF
Sbjct: 561 IIPRVDKPFKTSIVFSLEEGPGQLFKALAVF 653
Score = 354 bits (767), Expect = 8e-097
Identities = 162/213 (76%), Positives = 170/213 (79%)
Frame = -2 / -3
Query: 814 TASALKSCPGPSSSEKTMLVLNGLSVRGMMGSRASIIKRVTLSLSS*IFSARTSNPYSLA 635
TASALKSCPGPSSSE TMLVLNGLS RG++GSRASIIKRVTL+ SS*IFSAR S+ S A
Sbjct: 649 TASALKSCPGPSSSENTMLVLNGLSTRGIIGSRASIIKRVTLTFSS*IFSARISSS*SSA 470
Query: 634 ANELAIAPVSWSICSATIFAAPAVSSTASLCSIPSDVNVCSHCARAWG*LIALFRFSIFT 455
ANELA APVSWSICSAT FAAPAVSSTA LCSIP+ V+VCSHCARAWG*L+ RFSIFT
Sbjct: 469 ANELATAPVSWSICSATNFAAPAVSSTACLCSIPNSVSVCSHCARAWG*LMTFLRFSIFT 290
Query: 454 PGLANKQWRTARRTSPTMCKLCRSKRS*FLCMLPPNEFSSGSTALSATQF*TA*NAVSKC 275
P LANKQ RTARRTSPTMC CRSKRS*F C+LPP FSSGSTALS QF*TA*NAVSKC
Sbjct: 289 PRLANKQCRTARRTSPTMCSSCRSKRS*FRCILPPKVFSSGSTALSTIQF*TA*NAVSKC 110
Query: 274 SQGTVSQFGXXXXXXXXXXXXAQPW*ATLRTIP 176
SQGTV QFG P ATL P
Sbjct: 109 SQGTVWQFG*AFFAAASLSLQGSPGKATLSPSP 11
Score = 287 bits (622), Expect = 8e-077
Identities = 131/211 (62%), Positives = 150/211 (71%)
Frame = -1 / -2
Query: 818 KYSKCLKELSRSFF**KDYACLKWFISARYDGFSS*HHKTGDIIFVILNILSKNIQSIQF 639
K KCL+ELS SFF *K A L+WFI+ RY+ F S HHK GDI F +LNI SKNIQ I+F
Sbjct: 653 KNCKCLEELSGSFFK*KHNASLEWFINPRYNRFPSKHHKAGDIDFFVLNIFSKNIQFIKF 474
Query: 638 SRQRTSNSTSVLEHMFCNNLCCTSSVVNSFSMLNS**C*CVLTLRKSLGMTHSTFQVFNL 459
S T NSTS+LEH+FCN LCCTSSV+NS SM NS C C+LTLRK LGMT +F+L
Sbjct: 473 SS**TGNSTSILEHLFCN*LCCTSSVINSLSMFNSQLCQCMLTLRKGLGMTDDIS*IFDL 294
Query: 458 HARIG**AMANRKANFSYNVQTMPKQKIIIPMYATSQ*ILKWQYGPISYPVLNSLKCSVE 279
H+ IG* AM N KAN S VQ MPK KII+PMY T+Q ILKWQY PI+YPVLNSLK S++
Sbjct: 293 HSTIG*QAMPNCKANLSNYVQLMPK*KIIVPMYTTAQSILKWQYCPINYPVLNSLKRSIK 114
Query: 278 VLTGHSLTIRVRLLCCCFAVRSSTTLVSNLE 186
VL GH L I + LLCC V NLE
Sbjct: 113 VLAGHCLAIWIGLLCCSLTVAPGKPW*GNLE 21
Score = 249 bits (538), Expect = 3e-065
Identities = 121/215 (56%), Positives = 140/215 (65%)
Frame = +2 / +2
Query: 167 GGGRYCSQGCLPGLCWSVQRSSSKEGVPEL*DCAL*ALRHCISGCSKLGS**GRTAT*EF 346
G R +QGCL Q SKEG+ +L D AL AL +C+SGCSKL S**G TAT*E+
Sbjct: 2 GTRRGGAQGCLTRASLERQ*GCSKEGLSKLPDSALRAL*YCVSGCSKLDS**GSTAT*EY 181
Query: 347 IGR*HT*EL*SFASA*FAHCRRSSPCGSPLLISQSWRED*KPEKCYESSPGSCAM*AHIN 526
GR*+T EL*SF SA* AH R SPC S LL+SQSW ED K +KC++SSPG CAM*A+ +
Sbjct: 182 FGR*YTSEL*SFTSA*AAHSWRGSPCSSALLVSQSWSEDRKSKKCHQSSPGPCAM*AYTD 361
Query: 527 ITRN*A*RSC*RHCWCSKDCCRTYAPGHWCYC*FVGG*TVWIGCSC*EYSG*QR*CHPFY 706
N* * SC* HCWCSK CRT AP +WC C F+ *T+ G SC*+YSG + CHP Y
Sbjct: 362 RVGN*T*TSC**HCWCSKVSCRTNAPRYWCCCQFISC*TL*TGYSC*KYSGRKSQCHPLY 541
Query: 707 DAXXXXXXXXXXXXI*DKHSLFTRRRTWTAL*GTC 811
DA I D H +FT RRT T L GTC
Sbjct: 542 DACSGTYYTSG**TIQD*HCVFT*RRTRTTLQGTC 646
Score = 217 bits (468), Expect = 1e-055
Identities = 115/208 (55%), Positives = 129/208 (62%)
Frame = -3 / -1
Query: 810 QVP*RAVQVLLLVKRLCLS*MVYQCEV*WVLELAS*NG*HYLCHPEYSQQEHPIHTV*PP 631
QVP*R V+VLL VK C S*MVYQ EV* V E AS*+G*H L PEY QQE+P+H V
Sbjct: 645 QVP*RVVRVLLQVKTQC*S*MVYQPEV**VPEQAS*SG*H*LFRPEYFQQEYPVHKVQQL 466
Query: 630 TN*Q*HQCPGAYVXXXXXXXXXXXXXXLYAQFLVMLMCAHIAQEPGDDS*HFSGFQSSRQ 451
N Q HQ GA+V +Y QF + + AHIAQ PGDD *HF F+SS
Sbjct: 465 MNWQQHQYLGAFVLQLTLLHQQCHQQLVYVQFPTLSVYAHIAQGPGDD**HFLDFRSSLH 286
Query: 450 DWLISNGEPQGELLLQCANYAEAKDHNSYVCYLPMNSQVAVRPYQLPSFEQPEMQCRSAH 271
DWL SN E QGE L CA +AE KDH+S V Y P SQVAV PYQL SFEQPE Q +SA
Sbjct: 285 DWLTSNAELQGEPLQLCAAHAEVKDHSSDVYYRPKYSQVAVLPYQLSSFEQPETQYQSAR 106
Query: 270 RAQSHNSGTPSLLLLRCTLQHNPGKQP* 187
RA S N PSLL C + +QP*
Sbjct: 105 RALSGNLDRPSLLQPHCRSREALVRQP* 22
Score = 210 bits (453), Expect = 2e-053
Identities = 105/190 (55%), Positives = 120/190 (63%)
Frame = +1 / +1
Query: 250 RIVRLCPVSTSTLHFRLFKTG*LIGPYCHLRIHWEVAYIGIMIFCFGIVCTL*EKFALRF 429
+I R CP ST L FRLFKTG*LIG YCHLRI W V YIG MIF FG+ CT E+FAL+F
Sbjct: 85 QIARQCPASTLILRFRLFKTG*LIGQYCHLRILWAVVYIGTMIFYFGMSCT*LERFALQF 264
Query: 430 AIAY*PILA*RLKT*KVL*VIPRLLRNVSTH*HH*ELSIEKXXXXXXXXXXXXXNICSRT 609
IA *PI+ *R K *++ VIPR LRNVS H* EL+I+K N CS+
Sbjct: 265 GIAC*PIVE*RSKI*EMSSVIPRPLRNVSIH*QSWELNIDKLLMTLLVQQS*LQNKCSKI 444
Query: 610 XXXXXXXXXXNCMDWMFLLRIFRMTKIMSPVL*C*LENPSYLALINHLRQA*SFH*KKDL 789
N M+W+FLL+IFR K MSP L*C L N YL LINH R A FH KKD
Sbjct: 445 LVLLPVH*LLNFMNWIFLLKIFRTKKSMSPAL*CLLGNLLYLGLINHSRLALCFHLKKDP 624
Query: 790 DSSLRHLLYL 819
D+S RHL +L
Sbjct: 625 DNSSRHLQFL 654
>gb|BI953204.1|BI953204 HVSMEm0011K04f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0011K04f, mRNA sequence
Length = 636
Score = 410 bits (890), Expect = e-114
Identities = 172/200 (86%), Positives = 186/200 (93%)
Frame = +3 / +1
Query: 249 PNCETVPCEHFDTAFQAVQNWVADRAVLPLENSLGGSIHRNYDLLLRHSLHIVGEVRLAV 428
P+CETVPCE+F+TAFQAV+NWVADRAVLPLENSLGGSIHRNYDLLLRH LHIVGEVRLAV
Sbjct: 37 PSCETVPCEYFETAFQAVENWVADRAVLPLENSLGGSIHRNYDLLLRHRLHIVGEVRLAV 216
Query: 429 RHCLLANPGVKIENLKSAMSHPQALAQCEHTLTSLGIEHREAVDDTAGAAKIVAEHMLQD 608
RHCLLAN GVKIENL+SAMSHPQALAQCE TLT LGIEHREAVDDTAGAAK +AE LQD
Sbjct: 217 RHCLLANRGVKIENLRSAMSHPQALAQCEQTLTKLGIEHREAVDDTAGAAKHIAEQNLQD 396
Query: 609 TGAIASSLAAKLYGLDVLAENIQDDKDNVTRFMMLAREPIIPRTDKPFKTSIVFSLEEGP 788
T A+ASS+AA+LYGLD+LAENIQDD DNVTRFMMLAREPIIPRTDKPFKTSIVFSLEE P
Sbjct: 397 TAAVASSVAAQLYGLDILAENIQDDADNVTRFMMLAREPIIPRTDKPFKTSIVFSLEERP 576
Query: 789 GQLFKALAVFALRDINLTKI 848
G +FK L +FAL+ INLTK+
Sbjct: 577 GPVFKGLWMFALKKINLTKM 636
Score = 308 bits (668), Expect = 3e-083
Identities = 147/200 (73%), Positives = 156/200 (78%)
Frame = -2 / -2
Query: 847 IFVRLMSLSANTASALKSCPGPSSSEKTMLVLNGLSVRGMMGSRASIIKRVTLSLSS*IF 668
I VRL+ AN LK+ PG SS E TMLVLNGLSVRG+MGSRASIIKRVTLS SS IF
Sbjct: 635 ILVRLIFFRANIQRPLKTGPGLSSRENTMLVLNGLSVRGIMGSRASIIKRVTLSASSWIF 456
Query: 667 SARTSNPYSLAANELAIAPVSWSICSATIFAAPAVSSTASLCSIPSDVNVCSHCARAWG* 488
SAR S+P S AA ELA A VSW CSA AAPAVSSTASLCSIPS V+VCSHCARAWG
Sbjct: 455 SARISSP*S*AATELATAAVSWRFCSAMCLAAPAVSSTASLCSIPSFVSVCSHCARAWGW 276
Query: 487 LIALFRFSIFTPGLANKQWRTARRTSPTMCKLCRSKRS*FLCMLPPNEFSSGSTALSATQ 308
L+AL RFSI TP A+KQ RTA RTSPTMC RS+RS* CMLPPNEFSSG+TA SATQ
Sbjct: 275 LMALRRFSILTPRFASKQCRTASRTSPTMCNRWRSRRS*LRCMLPPNEFSSGNTARSATQ 96
Query: 307 F*TA*NAVSKCSQGTVSQFG 248
F TA AVSK SQGTVSQ G
Sbjct: 95 FSTAWKAVSKYSQGTVSQLG 36
Score = 232 bits (502), Expect = 3e-060
Identities = 111/204 (54%), Positives = 137/204 (67%)
Frame = -1 / -1
Query: 848 NLCEVDVPQCKYSKCLKELSRSFF**KDYACLKWFISARYDGFSS*HHKTGDIIFVILNI 669
+L +V+ Q K+ K L+ SRSFF + YA L+W ISAR +GF S HHK G II VIL+I
Sbjct: 636 HLGKVNFLQSKHPKALENRSRSFFKREHYASLEWLISARNNGFPSQHHKAGHIIRVILDI 457
Query: 668 LSKNIQSIQFSRQRTSNSTSVLEHMFCNNLCCTSSVVNSFSMLNS**C*CVLTLRKSLGM 489
KNIQSI+ S RT N + VLE +FCN L C V+NSFSM NS* C C+LTL KSLGM
Sbjct: 456 FCKNIQSIKLSSHRTGNCSGVLEILFCNVLGCAGCVINSFSMFNS*LCQCLLTLCKSLGM 277
Query: 488 THSTFQVFNLHARIG**AMANRKANFSYNVQTMPKQKIIIPMYATSQ*ILKWQYGPISYP 309
H T QV NL I AM NR+ + NVQ M ++I+ MYAT+Q*+L WQ+G IS+P
Sbjct: 276 AHGTPQVLNLDTTIRQQAMPNREPYLANNVQPMA**EVIVAMYATTQ*VL*WQHGAISHP 97
Query: 308 VLNSLKCSVEVLTGHSLTIRVRLL 237
+ NSL+ +EVL GH L RV LL
Sbjct: 96 IFNSLEGGLEVLAGHGLAARVGLL 25
Score = 188 bits (406), Expect = 5e-047
Identities = 96/210 (45%), Positives = 124/210 (59%)
Frame = +2 / +3
Query: 218 VQRSSSKEGVPEL*DCAL*ALRHCISGCSKLGS**GRTAT*EFIGR*HT*EL*SFASA*F 397
+QR +EG+PEL D AL LR + GC KLG * R AT E IG *HT +L* +
Sbjct: 6 LQRGRRQEGLPELRDRALRVLRDRLPGC*KLGG*SRRVATRELIGW*HTSQL*PPTTPSV 185
Query: 398 AHCRRSSPCGSPLLISQSWRED*KPEKCYESSPGSCAM*AHINITRN*A*RSC*RHCWCS 577
AHC R + GS LL +SW +D*+P +C+E SP SC +*A+ + RN* *RSC* H S
Sbjct: 186 AHCWRGTARGSALLAGESWCQD*EPAECHEPSPSSCTV*ANTDKARN*T*RSC**HSRRS 365
Query: 578 KDCCRTYAPGHWCYC*FVGG*TVWIGCSC*EYSG*QR*CHPFYDAXXXXXXXXXXXXI*D 757
+ CRT +P H C C F G T+W G SC +Y G*+ *C P YDA I D
Sbjct: 366 QAHCRTKSPRHRCSCQFGGCSTLWTGYSCRKYPG*RG*CDPLYDAGSGTHYSSH**AIQD 545
Query: 758 KHSLFTRRRTWTAL*GTCCICTEGHQPHKD 847
HS+ + R+TWT G +C+E + P++D
Sbjct: 546 *HSVLS*RKTWTGFQGPLDVCSEEN*PYQD 635
Score = 162 bits (349), Expect = 3e-039
Identities = 90/191 (47%), Positives = 105/191 (54%)
Frame = +1 / +2
Query: 220 TAKXXXXXXTRIVRLCPVSTSTLHFRLFKTG*LIGPYCHLRIHWEVAYIGIMIFCFGIVC 399
TA+ TR R CP STS RL K G LI P CH R HW VAYI M + I C
Sbjct: 8 TARPPPRRPTRAARPCPASTSRPPSRLLKIGWLIAPCCH*RTHWVVAYIATMTSYYAIGC 187
Query: 400 TL*EKFALRFAIAY*PILA*RLKT*KVL*VIPRLLRNVSTH*HH*ELSIEKXXXXXXXXX 579
TL ++ RF IA I+ RL+T V * IP+LL +VS H* *EL+IEK
Sbjct: 188 TLLARYGSRFGIACWRIVVSRLRTCGVP*AIPKLLHSVSKH*QS*ELNIEKLLMTQPAQP 367
Query: 580 XXXXNICSRTXXXXXXXXXXNCMDWMFLLRIFRMTKIMSPVL*C*LENPSYLALINHLRQ 759
N S+T N MDW+FL +I RMT+IM P L*C L NP +LALI+H R
Sbjct: 368 STLQNKISKTPLQLPVRWLLNFMDWIFLQKISRMTRIM*PAL*CWLGNPLFLALISHSRL 547
Query: 760 A*SFH*KKDLD 792
A* KKDLD
Sbjct: 548 A*CSLLKKDLD 580
Score = 99.1 bits (210), Expect(2) = 3e-040
Identities = 49/109 (44%), Positives = 60/109 (55%)
Frame = -3 / -3
Query: 546 YAQFLVMLMCAHIAQEPGDDS*HFSGFQSSRQDWLISNGEPQGELLLQCANYAEAKDHNS 367
Y QFL + + AH QE GD S H +G QS D SN EP+ QCA H+
Sbjct: 334 YVQFLALSVFAHTVQELGDGSWHSAGSQS*HHDSPASNAEPRAVPRQQCATDGVVGGHSC 155
Query: 366 YVCYLPMNSQVAVRPYQLPSFEQPEMQCRSAHRAQSHNSGTPSLLLLRC 220
VCY PM+S VA R Q P+F+QP + RS RA+S +SG PS RC
Sbjct: 154 DVCYHPMSSLVATRRDQPPNFQQPGRRSRSTRRARSRSSGRPSWRRPRC 8
Score = 87.2 bits (184), Expect(2) = 3e-040
Identities = 45/85 (52%), Positives = 53/85 (62%)
Frame = -3 / -3
Query: 846 SL*G*CPSVQIQQVP*RAVQVLLLVKRLCLS*MVYQCEV*WVLELAS*NG*HYLCHPEYS 667
S *G* S Q + P*+ VQV L + LC S*M YQCE *WV E AS*+G HY HP Y
Sbjct: 634 SW*G*FSSEQTSKGP*KPVQVFLQERTLC*S*MAYQCEE*WVPEPAS*SGSHYPRHPGYF 455
Query: 666 QQEHPIHTV*PPTN*Q*HQCPGAYV 592
QE+P+H V P N Q +C G +V
Sbjct: 454 LQEYPVHKVEQPPNWQLQRCLGDFV 380
>gb|BG415787.1|BG415787 HVSMEk0007M21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0007M21f, mRNA sequence
Length = 638
Score = 171 bits (368), Expect(3) = 4e-086
Identities = 65/94 (69%), Positives = 79/94 (84%)
Frame = +3 / +1
Query: 258 ETVPCEHFDTAFQAVQNWVADRAVLPLENSLGGSIHRNYDLLLRHSLHIVGEVRLAVRHC 437
+ +PC+ F+ AFQAV+ W+ADRAVLP+ENSLGGSIH NYDLLLRH LHIVGEV+L V HC
Sbjct: 1 DAIPCDQFEVAFQAVELWIADRAVLPVENSLGGSIHANYDLLLRHRLHIVGEVQLPVHHC 180
Query: 438 LLANPGVKIENLKSAMSHPQALAQCEHTLTSLGI 539
LL PGV+ EN+ +SHPQALAQCEHT+T +G+
Sbjct: 181 LLTLPGVRKENITRVISHPQALAQCEHTITRMGL 282
Score = 128 bits (275), Expect(3) = 4e-086
Identities = 55/76 (72%), Positives = 67/76 (88%)
Frame = +3 / +1
Query: 546 REAVDDTAGAAKIVAEHMLQDTGAIASSLAAKLYGLDVLAENIQDDKDNVTRFMMLAREP 725
REA DDTAGAA+ VA + L+DT AIASS AA+LYG+++LA+ IQDD NVTRF+MLAREP
Sbjct: 292 REAFDDTAGAAEYVATNGLRDTVAIASSRAAELYGMEILADGIQDDCGNVTRFVMLAREP 471
Query: 726 IIPRTDKPFKTSIVFS 773
I+PRTD+PFKTSIVF+
Sbjct: 472 IVPRTDRPFKTSIVFA 519
Score = 107 bits (228), Expect(3) = 3e-047
Identities = 52/90 (57%), Positives = 58/90 (64%)
Frame = -2 / -1
Query: 535 PSDVNVCSHCARAWG*LIALFRFSIFTPGLANKQWRTARRTSPTMCKLCRSKRS*FLCML 356
P V VCSHCA A G L+ FS+ TPG +QW T TSPTM RS+RS*F ML
Sbjct: 278 PMRVMVCSHCASACGWLMTRVMFSLRTPGSVRRQWCTGSCTSPTMWSRWRSRRS*FAWML 99
Query: 355 PPNEFSSGSTALSATQF*TA*NAVSKCSQG 266
PP+EFS+GSTA SA Q TA NA S SQG
Sbjct: 98 PPSEFSTGSTARSAIQSSTAWNATSNWSQG 9
Score = 88.6 bits (187), Expect(3) = 3e-047
Identities = 50/77 (64%), Positives = 52/77 (67%)
Frame = -2 / -1
Query: 778 SSEKTMLVLNGLSVRGMMGSRASIIKRVTLSLSS*IFSARTSNPYSLAANELAIAPVSWS 599
S TMLVLNGLSVRG MGS ASI RVTL SS I SAR S PYS AA + A+A VS
Sbjct: 524 SCANTMLVLNGLSVRGTMGSLASITNRVTLPQSSCIPSARISMPYSSAARDDAMATVSRR 345
Query: 598 ICSATIFAAPAVSSTAS 548
AT AAPAVSS AS
Sbjct: 344 PLVATYSAAPAVSSKAS 294
Score = 58.3 bits (121), Expect(3) = 4e-086
Identities = 23/36 (63%), Positives = 28/36 (77%)
Frame = +3 / +1
Query: 777 EEGPGQLFKALAVFALRDINLTKIESRPHKERPLRV 884
+EG LFK L+ FALRDI LTKIES PH+ RP+R+
Sbjct: 526 KEGTSVLFKVLSAFALRDITLTKIESSPHRHRPIRL 633
Score = 32.2 bits (64), Expect(3) = 3e-047
Identities = 17/36 (47%), Positives = 18/36 (50%)
Frame = -2 / -1
Query: 880 RRGLSLCGRLSIFVRLMSLSANTASALKSCPGPSSS 773
R G CG LSI V +MS A S L S PS S
Sbjct: 629 RMGRWRCGELSILVSVMSRKAKAESTLNSTEVPSLS 522
>gb|BU985441.1|BU985441 HF07D15r HF Hordeum vulgare subsp. vulgare cDNA clone HF07D15
5-PRIME, mRNA sequence
Length = 411
Score = 275 bits (596), Expect = 3e-073
Identities = 115/135 (85%), Positives = 126/135 (93%)
Frame = +3 / +1
Query: 339 ENSLGGSIHRNYDLLLRHSLHIVGEVRLAVRHCLLANPGVKIENLKSAMSHPQALAQCEH 518
E++LGGSIHRNYDLLLRH LHIVGEVRLAVRHCLLAN GVKIENL++ +SHPQALAQCEH
Sbjct: 7 EDTLGGSIHRNYDLLLRHELHIVGEVRLAVRHCLLANRGVKIENLRNVISHPQALAQCEH 186
Query: 519 TLTSLGIEHREAVDDTAGAAKIVAEHMLQDTGAIASSLAAKLYGLDVLAENIQDDKDNVT 698
TLT LGIEHR+AVDDTAGAAK+VAE MLQDTGA+ASSLAA+LY LD+LAENIQD+K N T
Sbjct: 187 TLTELGIEHRQAVDDTAGAAKLVAEQMLQDTGAVASSLAAELYELDILAENIQDEKVNAT 366
Query: 699 RFMMLAREPIIPRTD 743
RFMMLAREPIIPR D
Sbjct: 367 RFMMLAREPIIPRVD 411
Score = 237 bits (511), Expect = 2e-061
Identities = 104/131 (79%), Positives = 112/131 (85%)
Frame = -2 / -2
Query: 742 SVRGMMGSRASIIKRVTLSLSS*IFSARTSNPYSLAANELAIAPVSWSICSATIFAAPAV 563
S RG++GSRASIIKRV L+ SS*IFSAR S+ S AANELA APVSWSICSAT FAAPAV
Sbjct: 410 STRGIIGSRASIIKRVALTFSS*IFSARISSS*SSAANELATAPVSWSICSATNFAAPAV 231
Query: 562 SSTASLCSIPSDVNVCSHCARAWG*LIALFRFSIFTPGLANKQWRTARRTSPTMCKLCRS 383
SSTA LCSIP+ V+VCSHCARAWG*L+ RFSIFTP LANKQ RTARRTSPTMC CRS
Sbjct: 230 SSTACLCSIPNSVSVCSHCARAWG*LMTFLRFSIFTPRLANKQCRTARRTSPTMCSSCRS 51
Query: 382 KRS*FLCMLPP 350
KRS*F C+LPP
Sbjct: 50 KRS*FRCILPP 18
Score = 185 bits (399), Expect = 4e-046
Identities = 85/135 (62%), Positives = 98/135 (72%)
Frame = -1 / -1
Query: 743 ISARYDGFSS*HHKTGDIIFVILNILSKNIQSIQFSRQRTSNSTSVLEHMFCNNLCCTSS 564
I+ RY+ F S HHK G I F +LNI SKNIQ I+FS T NSTS+LEH+FCN LCCTSS
Sbjct: 411 INPRYNRFPSKHHKAGGIDFFVLNIFSKNIQFIKFSS**TGNSTSILEHLFCN*LCCTSS 232
Query: 563 VVNSFSMLNS**C*CVLTLRKSLGMTHSTFQVFNLHARIG**AMANRKANFSYNVQTMPK 384
V+NS SM NS C C+LTLRK LGMT +F+LH+ IG* AM N KAN S VQ MPK
Sbjct: 231 VINSLSMFNSQLCQCMLTLRKGLGMTDDIS*IFDLHSTIG*QAMPNCKANLSNYVQLMPK 52
Query: 383 QKIIIPMYATSQ*IL 339
KII+PMY T+Q IL
Sbjct: 51 *KIIVPMYTTAQSIL 7
Score = 173 bits (372), Expect = 2e-042
Identities = 75/123 (60%), Positives = 88/123 (71%)
Frame = +2 / +3
Query: 344 FIGR*HT*EL*SFASA*FAHCRRSSPCGSPLLISQSWRED*KPEKCYESSPGSCAM*AHI 523
+ GR*+T EL*SF SA* AH R SPC S LL+SQSW ED K +KC++SSPG CAM*A+
Sbjct: 12 YFGR*YTSEL*SFTSA*AAHSWRGSPCSSALLVSQSWSEDRKSKKCHQSSPGPCAM*AYT 191
Query: 524 NITRN*A*RSC*RHCWCSKDCCRTYAPGHWCYC*FVGG*TVWIGCSC*EYSG*QR*CHPF 703
+ N* * SC* HCWCSK CRT AP +WC C F+ *T+ G SC*+YSG + CHP
Sbjct: 192 DRVGN*T*TSC**HCWCSKVSCRTNAPRYWCCCQFISC*TL*TGYSC*KYSGRKSQCHPL 371
Query: 704 YDA 712
YDA
Sbjct: 372 YDA 380
Score = 128 bits (274), Expect = 8e-029
Identities = 67/131 (51%), Positives = 77/131 (58%)
Frame = -3 / -3
Query: 741 QCEV*WVLELAS*NG*HYLCHPEYSQQEHPIHTV*PPTN*Q*HQCPGAYVXXXXXXXXXX 562
Q EV* V E AS*+G H L PEY QQE+P+H V N Q HQ GA+V
Sbjct: 409 QPEV**VPEQAS*SGWH*LFRPEYFQQEYPVHKVQQLMNWQQHQYLGAFVLQLTLLHQQC 230
Query: 561 XXXXLYAQFLVMLMCAHIAQEPGDDS*HFSGFQSSRQDWLISNGEPQGELLLQCANYAEA 382
+Y QF + + AHIAQ PGDD *HF F+SS DWL SN E QGE L CA +AE
Sbjct: 229 HQQLVYVQFPTLSVYAHIAQGPGDD**HFLDFRSSLHDWLTSNAELQGEPLQLCAAHAEV 50
Query: 381 KDHNSYVCYLP 349
KDH+S V Y P
Sbjct: 49 KDHSSDVYYRP 17
Score = 123 bits (263), Expect = 3e-027
Identities = 66/134 (49%), Positives = 78/134 (58%)
Frame = +1 / +2
Query: 340 RIHWEVAYIGIMIFCFGIVCTL*EKFALRFAIAY*PILA*RLKT*KVL*VIPRLLRNVST 519
RI W V YIG MIF FG+ CT E+FAL+F IA *PI+ *R K *++ VIPR LRNVS
Sbjct: 8 RILWAVVYIGTMIFYFGMSCT*LERFALQFGIAC*PIVE*RSKI*EMSSVIPRPLRNVSI 187
Query: 520 H*HH*ELSIEKXXXXXXXXXXXXXNICSRTXXXXXXXXXXNCMDWMFLLRIFRMTKIMSP 699
H* EL+I+K N CS+ N M+W+FLL+IFR K M P
Sbjct: 188 H*QSWELNIDKLLMTLLVQQS*LQNKCSKILVLLPVH*LLNFMNWIFLLKIFRTKKSMPP 367
Query: 700 VL*C*LENPSYLAL 741
L*C L N YL L
Sbjct: 368 AL*CLLGNLLYLGL 409
>gb|BM371147.2|BM371147 EBro04_SQ003_K01_R root, 3 week, salt-stressed, cv Optic, EBro04
Hordeum vulgare subsp. vulgare cDNA clone
EBro04_SQ003_K01 5', mRNA sequence
Length = 584
Score = 130 bits (278), Expect(4) = 3e-067
Identities = 55/76 (72%), Positives = 67/76 (88%)
Frame = +3 / +2
Query: 546 REAVDDTAGAAKIVAEHMLQDTGAIASSLAAKLYGLDVLAENIQDDKDNVTRFMMLAREP 725
REA DDTAGAA+ VA + L+DT AIASS AA+LYG+++LA+ IQDD NVTRF+MLAREP
Sbjct: 176 REAFDDTAGAAEYVATNGLRDTAAIASSRAAELYGMEILADGIQDDCGNVTRFVMLAREP 355
Query: 726 IIPRTDKPFKTSIVFS 773
I+PRTD+PFKTSIVF+
Sbjct: 356 IVPRTDRPFKTSIVFA 403
Score = 99.6 bits (211), Expect(4) = 3e-067
Identities = 39/55 (70%), Positives = 46/55 (83%)
Frame = +3 / +2
Query: 375 DLLLRHSLHIVGEVRLAVRHCLLANPGVKIENLKSAMSHPQALAQCEHTLTSLGI 539
DLLLRH LHIVGEV+L V HCLLA PGV+ EN+ +SHPQALAQCEHT+T +G+
Sbjct: 2 DLLLRHRLHIVGEVQLPVHHCLLALPGVRKENITRVISHPQALAQCEHTITRMGL 166
Score = 88.6 bits (187), Expect(3) = 4e-036
Identities = 50/77 (64%), Positives = 52/77 (67%)
Frame = -2 / -3
Query: 778 SSEKTMLVLNGLSVRGMMGSRASIIKRVTLSLSS*IFSARTSNPYSLAANELAIAPVSWS 599
S TMLVLNGLSVRG MGS ASI RVTL SS I SAR S PYS AA + A+A VS
Sbjct: 408 SCANTMLVLNGLSVRGTMGSLASITNRVTLPQSSCIPSARISMPYSSAARDDAMAAVSRR 229
Query: 598 ICSATIFAAPAVSSTAS 548
AT AAPAVSS AS
Sbjct: 228 PLVATYSAAPAVSSKAS 178
Score = 66.6 bits (139), Expect(3) = 4e-036
Identities = 30/54 (55%), Positives = 34/54 (62%)
Frame = -2 / -3
Query: 535 PSDVNVCSHCARAWG*LIALFRFSIFTPGLANKQWRTARRTSPTMCKLCRSKRS 374
P V VCSHCARA G L+ FS+ TPG A +QW T TSPTM RS+RS
Sbjct: 162 PMRVMVCSHCARACGWLMTRVMFSLRTPGSARRQWCTGSCTSPTMWSRWRSRRS 1
Score = 56.5 bits (117), Expect(4) = 3e-067
Identities = 22/34 (64%), Positives = 26/34 (76%)
Frame = +3 / +2
Query: 777 EEGPGQLFKALAVFALRDINLTKIESRPHKERPL 878
+EG LFK L+ FA RDI LTKIESRPH+ RP+
Sbjct: 410 KEGTSVLFKVLSAFAFRDITLTKIESRPHRHRPI 511
Score = 35.4 bits (71), Expect(3) = 4e-036
Identities = 17/30 (56%), Positives = 18/30 (60%)
Frame = -2 / -3
Query: 862 CGRLSIFVRLMSLSANTASALKSCPGPSSS 773
CGRLSI V +MS A S LKS PS S
Sbjct: 495 CGRLSILVSVMSRKAKAESTLKSTEVPSLS 406
Score = 27.6 bits (54), Expect(4) = 3e-067
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3 / +3
Query: 882 VSDDCSSLLKNFDYLFYVDLEA 947
V D K+F+Y+FYVD +A
Sbjct: 519 VDDANRGTAKHFEYMFYVDFQA 584
>gb|CB881351.1|CB881351 HM09I17w HM Hordeum vulgare subsp. vulgare cDNA clone HM09I17
3-PRIME, mRNA sequence
Length = 612
Score = 151 bits (325), Expect(2) = 4e-061
Identities = 64/71 (90%), Positives = 67/71 (94%)
Frame = +3 / -2
Query: 690 NVTRFMMLAREPIIPRTDKPFKTSIVFSLEEGPGQLFKALAVFALRDINLTKIESRPHKE 869
NVTRFMMLAREPIIPR DKPFKTSIVFSLEEGPGQLFKALAVFALR+INLTKIESRPHK+
Sbjct: 611 NVTRFMMLAREPIIPRVDKPFKTSIVFSLEEGPGQLFKALAVFALREINLTKIESRPHKK 432
Query: 870 RPLRVSDDCSS 902
RP RV+DD S
Sbjct: 431 RPFRVADDTFS 399
Score = 127 bits (271), Expect(2) = 3e-041
Identities = 56/65 (86%), Positives = 60/65 (92%)
Frame = -2 / +1
Query: 883 TRRGLSLCGRLSIFVRLMSLSANTASALKSCPGPSSSEKTMLVLNGLSVRGMMGSRASII 704
TR+GL LCGRLSI VRL+SLSA TASALKSCPGPSSSE TMLVLNGLS RG++GSRASII
Sbjct: 418 TRKGLFLCGRLSILVRLISLSAKTASALKSCPGPSSSENTMLVLNGLSTRGIIGSRASII 597
Query: 703 KRVTL 689
KRVTL
Sbjct: 598 KRVTL 612
Score = 107 bits (229), Expect(2) = 2e-033
Identities = 44/69 (63%), Positives = 52/69 (75%)
Frame = -1 / +3
Query: 896 TVIRYPKRPFLMWTTFNLCEVDVPQCKYSKCLKELSRSFF**KDYACLKWFISARYDGFS 717
++I Y KRPFL+WT F+L EVD PQCK KCL+ELS SFF *K A L+WFI+ RY+ F
Sbjct: 405 SIISYTKRPFLVWTAFDLGEVDFPQCKNCKCLEELSGSFFK*KHNASLEWFINPRYNRFP 584
Query: 716 S*HHKTGDI 690
S HHK GDI
Sbjct: 585 SKHHKAGDI 611
Score = 104 bits (222), Expect(2) = 4e-061
Identities = 46/58 (79%), Positives = 50/58 (86%)
Frame = +3 / -2
Query: 888 DDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDVNEP*IES 1061
D S+ +K FDYLFYVDLEASMADPKTQNAL NL+EFATF RVLGSYPTDV+E *I S
Sbjct: 410 DTFSTRVKYFDYLFYVDLEASMADPKTQNALRNLEEFATFSRVLGSYPTDVSEA*IAS 237
Score = 89.5 bits (189), Expect(2) = 4e-027
Identities = 44/67 (65%), Positives = 46/67 (68%)
Frame = +1 / -3
Query: 691 MSPVL*C*LENPSYLALINHLRQA*SFH*KKDLDSSLRHLLYLH*GTSTSQRLKVVHIRK 870
MSP L*C L N YL LINH R A FH KKD D+S RHL +LH*G STS R K VH RK
Sbjct: 610 MSPAL*CLLGNLLYLGLINHSRLALCFHLKKDPDNSSRHLQFLH*GKSTSPRSKAVHTRK 431
Query: 871 GLFGYLM 891
GLF LM
Sbjct: 430 GLFV*LM 410
Score = 62.5 bits (130), Expect(2) = 3e-041
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = -2 / +1
Query: 1060 LSI*GSFTSVG*LPRTLKNVANSFRFPRAF*VFGSAIDA 944
L+I S TSVG*LPRTL+ VANS +F RAF*VFGSAIDA
Sbjct: 238 LAIQASLTSVG*LPRTLEKVANSSKFLRAF*VFGSAIDA 354
Score = 56.1 bits (116), Expect(2) = 2e-033
Identities = 28/49 (57%), Positives = 33/49 (67%)
Frame = -3 / +2
Query: 1050 KVHSHL*DSFQELLKMLQTPSDFQEHSESLDQPLMLQGQHKKGNQSSLR 904
K+ S L*DS LLK L+TP +F E SE LDQP QG HK G+QS+ R
Sbjct: 248 KLRSRL*DSSLGLLKRLRTPPNF*ERSEFLDQP*TPQGPHKTGSQST*R 394
Score = 52.8 bits (109), Expect(2) = 4e-027
Identities = 31/66 (46%), Positives = 38/66 (57%)
Frame = +1 / -3
Query: 898 PRS*RTLITFFMLTLKHQWLIQRLRMLLEI*RSLQHF*EFLEAILQM*MNLRLKVLQITY 1077
P + TL T FM TL+ WLIQ+LR L EI RS Q F E AILQ * L +VL +
Sbjct: 400 PHASSTLTTCFMWTLRRLWLIQKLRTLSEIWRSSQPFQES*GAILQT*AKLE*QVLAADH 221
Query: 1078 WISRNR 1095
+ N+
Sbjct: 220 MLVINQ 203
>gb|BF257794.3|BF257794 HVSMEf0013O05f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0013O05f, mRNA sequence
Length = 524
Score = 188 bits (405), Expect(2) = 7e-055
Identities = 75/119 (63%), Positives = 89/119 (74%)
Frame = +3 / +1
Query: 177 GIVLKVAYQGCXXXXXXXXXXXXXPNCETVPCEHFDTAFQAVQNWVADRAVLPLENSLGG 356
G L+VAYQG P + +PC+ F+ AFQAV+NW+ADRAVLP+ENSLGG
Sbjct: 52 GSELRVAYQGMPGAYSEKASVKAYPGSDAIPCDQFEVAFQAVENWIADRAVLPVENSLGG 231
Query: 357 SIHRNYDLLLRHSLHIVGEVRLAVRHCLLANPGVKIENLKSAMSHPQALAQCEHTLTSL 533
SIHRNYDLLLRH LHIVGEV+L V HCLLA PGV+ E++ +SHPQALAQCEHTLT +
Sbjct: 232 SIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKEDVTRVISHPQALAQCEHTLTRM 408
Score = 125 bits (267), Expect(2) = 9e-032
Identities = 59/117 (50%), Positives = 70/117 (59%)
Frame = -2 / -1
Query: 526 VNVCSHCARAWG*LIALFRFSIFTPGLANKQWRTARRTSPTMCKLCRSKRS*FLCMLPPN 347
V+VCSHCA A G L+ S+ TPG A +QW T TSPT+C+ CRS+RS* MLPP+
Sbjct: 401 VSVCSHCASACGWLMTRVTSSLRTPGSARRQWCTGSCTSPTICRRCRSRRS*LRWMLPPS 222
Query: 346 EFSSGSTALSATQF*TA*NAVSKCSQGTVSQFGXXXXXXXXXXXXAQPW*ATLRTIP 176
EFS+GSTA SA QF TA NA S S G S+ G PW*AT + P
Sbjct: 221 EFSTGSTARSAIQFSTAWNATSNWSHGMASEPGYAFTDAFSLYAPGMPW*ATRSSEP 51
Score = 46.9 bits (96), Expect(2) = 7e-055
Identities = 22/32 (68%), Positives = 26/32 (81%)
Frame = +3 / +1
Query: 546 REAVDDTAGAAKIVAEHMLQDTGAIASSLAAK 641
REA DDTAGAA+ VA + L+DT AIASS AA+
Sbjct: 427 REAFDDTAGAAEYVAANGLRDTAAIASSRAAE 522
Score = 32.7 bits (65), Expect(2) = 9e-032
Identities = 19/36 (52%), Positives = 23/36 (63%)
Frame = -2 / -1
Query: 643 SLAANELAIAPVSWSICSATIFAAPAVSSTASLCSI 536
S AA E A+A VS +AT AAPAVSS AS ++
Sbjct: 524 SSAAREDAMAAVSRRPLAATYSAAPAVSSKASRAAL 417
>gb|CA027506.1|CA027506 HZ59C11r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59C11
5-PRIME, mRNA sequence
Length = 469
Score = 194 bits (418), Expect(2) = 3e-050
Identities = 76/121 (62%), Positives = 91/121 (75%)
Frame = +3 / +3
Query: 177 GIVLKVAYQGCXXXXXXXXXXXXXPNCETVPCEHFDTAFQAVQNWVADRAVLPLENSLGG 356
G L+VAYQG P C+ +PC+ F+ AFQAV+ W+ADRAVLP+ENSLGG
Sbjct: 57 GSELRVAYQGVPGAYSEKAAGKAYPGCDAIPCDQFEVAFQAVELWIADRAVLPVENSLGG 236
Query: 357 SIHRNYDLLLRHSLHIVGEVRLAVRHCLLANPGVKIENLKSAMSHPQALAQCEHTLTSLG 536
SIHRNYDLLLRH LHIVGEV+L V HCLLA PGV+ EN+ +SHPQALAQCEHT+T +G
Sbjct: 237 SIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVRKENITRVISHPQALAQCEHTITRMG 416
Query: 537 I 539
+
Sbjct: 417 L 419
Score = 115 bits (246), Expect = 6e-025
Identities = 60/120 (50%), Positives = 67/120 (55%)
Frame = -2 / -1
Query: 535 PSDVNVCSHCARAWG*LIALFRFSIFTPGLANKQWRTARRTSPTMCKLCRSKRS*FLCML 356
P V VCSHCA A G L+ FS+ TPG A +QW T TSPTM RS+RS* ML
Sbjct: 415 PMRVMVCSHCASACGWLMTRVMFSLRTPGSARRQWCTGSCTSPTMWSRWRSRRS*LRWML 236
Query: 355 PPNEFSSGSTALSATQF*TA*NAVSKCSQGTVSQFGXXXXXXXXXXXXAQPW*ATLRTIP 176
PP+EFS+GSTA SA Q TA NA S SQG SQ G PW AT + P
Sbjct: 235 PPSEFSTGSTARSAIQSSTAWNATSNWSQGMASQPG*ALPAAFSLYAPGTPWYATRSSEP 56
Score = 25.4 bits (49), Expect(2) = 3e-050
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +3 / +3
Query: 546 REAVDDTAGAAK 581
REA DDTAGAA+
Sbjct: 429 REAFDDTAGAAE 464
>gb|AJ462529.1|AJ462529 AJ462529 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200021A01F1, mRNA sequence
Length = 360
Score = 104 bits (222), Expect(2) = 2e-045
Identities = 46/58 (79%), Positives = 50/58 (86%)
Frame = +3 / +3
Query: 888 DDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDVNEP*IES 1061
D S+ +K FDYLFYVDLEASMADPKTQNAL NL+EFATF RVLGSYPTDV+E *I S
Sbjct: 135 DTFSTRVKYFDYLFYVDLEASMADPKTQNALRNLEEFATFSRVLGSYPTDVSEA*IAS 308
Score = 99.1 bits (210), Expect(2) = 2e-045
Identities = 42/48 (87%), Positives = 45/48 (93%)
Frame = +3 / +3
Query: 759 SIVFSLEEGPGQLFKALAVFALRDINLTKIESRPHKERPLRVSDDCSS 902
SIVFSLEEGPGQLFKALAVFALR+INLTKIESRPHK+RP RV+DD S
Sbjct: 3 SIVFSLEEGPGQLFKALAVFALREINLTKIESRPHKKRPFRVADDTFS 146
Score = 83.5 bits (176), Expect(2) = 3e-028
Identities = 36/42 (85%), Positives = 38/42 (90%)
Frame = -2 / -3
Query: 883 TRRGLSLCGRLSIFVRLMSLSANTASALKSCPGPSSSEKTML 758
TR+GL LCGRLSI VRL+SLSA TASALKSCPGPSSSE TML
Sbjct: 127 TRKGLFLCGRLSILVRLISLSAKTASALKSCPGPSSSENTML 2
Score = 72.1 bits (151), Expect(2) = 7e-023
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = -1 / -2
Query: 896 TVIRYPKRPFLMWTTFNLCEVDVPQCKYSKCLKELSRSFF**KDYA 759
++I Y KRPFL+WT F+L EVD PQCK KCL+ELS SFF *K A
Sbjct: 140 SIISYTKRPFLVWTAFDLGEVDFPQCKNCKCLEELSGSFFK*KHNA 3
Score = 62.5 bits (130), Expect(2) = 3e-028
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = -2 / -3
Query: 1060 LSI*GSFTSVG*LPRTLKNVANSFRFPRAF*VFGSAIDA 944
L+I S TSVG*LPRTL+ VANS +F RAF*VFGSAIDA
Sbjct: 307 LAIQASLTSVG*LPRTLEKVANSSKFLRAF*VFGSAIDA 191
Score = 56.1 bits (116), Expect(2) = 7e-023
Identities = 28/49 (57%), Positives = 33/49 (67%)
Frame = -3 / -1
Query: 1050 KVHSHL*DSFQELLKMLQTPSDFQEHSESLDQPLMLQGQHKKGNQSSLR 904
K+ S L*DS LLK L+TP +F E SE LDQP QG HK G+QS+ R
Sbjct: 297 KLRSRL*DSSLGLLKRLRTPPNF*ERSEFLDQP*TPQGPHKTGSQST*R 151
>gb|BM443421.2|BM443421 EBro02_SQ005_B05_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ005_B05 5', mRNA sequence
Length = 373
Score = 76.7 bits (161), Expect(3) = 3e-039
Identities = 29/53 (54%), Positives = 40/53 (75%)
Frame = +3 / +1
Query: 882 VSDDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDV 1040
V D K+F+Y+FYVD +AS+A+P+ QNAL ++EF +FLRVLGSYP D+
Sbjct: 196 VDDANRGTAKHFEYMFYVDFQASLAEPRAQNALAEVQEFTSFLRVLGSYPMDM 354
Score = 64.3 bits (134), Expect(3) = 3e-039
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = +3 / +1
Query: 777 EEGPGQLFKALAVFALRDINLTKIESRPHKERPLRVSDDCS 899
+EG LFK L+ FA RDI LTKIESRPH+ RP+R+ DD +
Sbjct: 88 KEGTSVLFKVLSAFAFRDITLTKIESRPHRHRPIRLVDDAN 210
Score = 60.2 bits (125), Expect(3) = 3e-039
Identities = 23/27 (85%), Positives = 27/27 (100%)
Frame = +3 / +1
Query: 693 VTRFMMLAREPIIPRTDKPFKTSIVFS 773
VTRF+MLAREPI+PRTD+PFKTSIVF+
Sbjct: 1 VTRFVMLAREPIVPRTDRPFKTSIVFA 81
>gb|BQ463672.1|BQ463672 HG01B07r HG Hordeum vulgare subsp. vulgare cDNA clone HG01B07
5-PRIME, mRNA sequence
Length = 412
Score = 151 bits (324), Expect = 1e-035
Identities = 60/95 (63%), Positives = 70/95 (73%)
Frame = +3 / +1
Query: 177 GIVLKVAYQGCXXXXXXXXXXXXXPNCETVPCEHFDTAFQAVQNWVADRAVLPLENSLGG 356
G L+VAYQG P + +PC+ F+ AFQAV+NW+ADRAVLP+ENSLGG
Sbjct: 124 GSELRVAYQGMPGAYSEKASVKAYPGSDAIPCDQFEVAFQAVENWIADRAVLPVENSLGG 303
Query: 357 SIHRNYDLLLRHSLHIVGEVRLAVRHCLLANPGVK 461
SIHRNYDLLLRH LHIVGEV+L V HCLLA PGV+
Sbjct: 304 SIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVR 408
Score = 103 bits (219), Expect = 3e-021
Identities = 48/94 (51%), Positives = 56/94 (59%)
Frame = -2 / -3
Query: 457 TPGLANKQWRTARRTSPTMCKLCRSKRS*FLCMLPPNEFSSGSTALSATQF*TA*NAVSK 278
TPG A +QW T TSPT+C+ CRS+RS* MLPP+EFS+GSTA SA QF TA NA S
Sbjct: 404 TPGSARRQWCTGSCTSPTICRRCRSRRS*LRWMLPPSEFSTGSTARSAIQFSTAWNATSN 225
Query: 277 CSQGTVSQFGXXXXXXXXXXXXAQPW*ATLRTIP 176
S G S+ G PW*AT + P
Sbjct: 224 WSHGMASEPGYAFTDAFSLYAPGMPW*ATRSSEP 123
>gb|CA027787.1|CA027787 HZ60A11r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ60A11
5-PRIME, mRNA sequence
Length = 306
Score = 150 bits (322), Expect = 2e-035
Identities = 59/95 (62%), Positives = 70/95 (73%)
Frame = +3 / +3
Query: 177 GIVLKVAYQGCXXXXXXXXXXXXXPNCETVPCEHFDTAFQAVQNWVADRAVLPLENSLGG 356
G L+VAYQG P C+ +PC+ F+ AFQAV+ W+ADR+VLP+ENSLGG
Sbjct: 18 GSELRVAYQGVPGAYSEKAAGKAYPGCDAIPCDQFEVAFQAVELWIADRSVLPVENSLGG 197
Query: 357 SIHRNYDLLLRHSLHIVGEVRLAVRHCLLANPGVK 461
SIHRNYDLLLRH LHIVGEV+L V HCLLA PGV+
Sbjct: 198 SIHRNYDLLLRHRLHIVGEVQLPVHHCLLALPGVR 302
>gb|BU979399.1|BU979399 HA16A13r HA Hordeum vulgare subsp. vulgare cDNA clone HA16A13
5-PRIME, mRNA sequence
Length = 315
Score = 121 bits (259), Expect(2) = 7e-035
Identities = 51/72 (70%), Positives = 62/72 (86%)
Frame = +3 / +1
Query: 546 REAVDDTAGAAKIVAEHMLQDTGAIASSLAAKLYGLDVLAENIQDDKDNVTRFMMLAREP 725
REA DDTAGAA+ VA + L+DT AIASS A +LYG+++LA+ IQDD NVTRF+MLAREP
Sbjct: 100 REAFDDTAGAAEYVATNGLRDTAAIASSRAVELYGMEILADGIQDDCGNVTRFVMLAREP 279
Query: 726 IIPRTDKPFKTS 761
I+PRTD+PFKTS
Sbjct: 280 IVPRTDRPFKTS 315
Score = 46.9 bits (96), Expect(2) = 7e-035
Identities = 16/25 (64%), Positives = 21/25 (84%)
Frame = +3 / +1
Query: 465 ENLKSAMSHPQALAQCEHTLTSLGI 539
EN+ +SHPQALAQCEHT+T +G+
Sbjct: 16 ENITRVISHPQALAQCEHTITRMGL 90
>gb|BU997534.1|BU997534 HI08E10r HI Hordeum vulgare subsp. vulgare cDNA clone HI08E10
5-PRIME, mRNA sequence
Length = 567
Score = 136 bits (291), Expect = 3e-031
Identities = 58/143 (40%), Positives = 77/143 (53%)
Frame = +3 / +3
Query: 174 DGIVLKVAYQGCXXXXXXXXXXXXXPNCETVPCEHFDTAFQAVQNWVADRAVLPLENSLG 353
DG L+VAYQG P CETVPC F A AV + RA+LP+E+++
Sbjct: 102 DGTGLRVAYQGAPGAYSEFAAKTALPGCETVPCRAFADALAAVDRGLVHRAILPVESTME 281
Query: 354 GSIHRNYDLLLRHSLHIVGEVRLAVRHCLLANPGVKIENLKSAMSHPQALAQCEHTLTSL 533
G+ RNYDLLLRH L + E+ L V +CLLA PGV+ ++ +SHP ALA C L L
Sbjct: 282 GTALRNYDLLLRHDLVVAQEINLFVHYCLLAMPGVRATEVRRVISHPMALAHCGRALARL 461
Query: 534 GIEHREAVDDTAGAAKIVAEHML 602
G++ D + + ML
Sbjct: 462 GVDREPVEDTAGAVEMLRSNMML 530
>gb|BQ134710.1|BQ134710 LP20067/1 Lemma/palea-enriched cDNA library from gelatinous stage
of kernel Hordeum vulgare subsp. vulgare cDNA clone
2-67/1 similar to Putative chorismate mutase/prephenate
dehydratase, mRNA sequence
Length = 286
Score = 84.5 bits (178), Expect(2) = 5e-029
Identities = 32/43 (74%), Positives = 40/43 (93%)
Frame = +3 / -2
Query: 645 YGLDVLAENIQDDKDNVTRFMMLAREPIIPRTDKPFKTSIVFS 773
YG+++LA+ IQDD NVTRF+MLAREPI+PRTD+PFKTSIVF+
Sbjct: 285 YGMEILADGIQDDCGNVTRFVMLAREPIVPRTDRPFKTSIVFA 157
Score = 64.3 bits (134), Expect(2) = 5e-029
Identities = 25/41 (60%), Positives = 31/41 (75%)
Frame = +3 / -2
Query: 777 EEGPGQLFKALAVFALRDINLTKIESRPHKERPLRVSDDCS 899
+EG LFK L+ FA RDI LTKIESRPH+ RP+R+ DD +
Sbjct: 150 KEGTSVLFKVLSAFAFRDITLTKIESRPHRHRPIRLVDDAN 28
>gb|BQ762795.1|BQ762795 EBro02_SQ006_I06_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ006_I06 5', mRNA sequence
Length = 455
Score = 74.4 bits (156), Expect(2) = 8e-025
Identities = 29/53 (54%), Positives = 39/53 (73%)
Frame = +3 / +3
Query: 882 VSDDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKEFATFLRVLGSYPTDV 1040
V D K+F+Y+FYVD +AS+A+P+ QNAL +EF +FLRVLGSYP D+
Sbjct: 96 VDDANRGTAKHFEYMFYVDFQASLAEPRAQNALAEGQEFTSFLRVLGSYPMDM 254
Score = 60.2 bits (125), Expect(2) = 8e-025
Identities = 23/35 (65%), Positives = 28/35 (80%)
Frame = +3 / +3
Query: 795 LFKALAVFALRDINLTKIESRPHKERPLRVSDDCS 899
LFK L+ FA RDI LTKIESRPH+ RP+R+ DD +
Sbjct: 6 LFKVLSAFAFRDITLTKIESRPHRHRPIRLVDDAN 110
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 597,807,694
Number of Sequences: 312970
Number of extensions: 9160453
Number of successful extensions: 63075
Number of sequences better than 1.0e-020: 18
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 17844658363
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)