TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950160.2.1
(846 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA li... 242 2e-079
gb|BJ467938.1|BJ467938 BJ467938 K. Sato unpublished cDNA li... 206 1e-052
gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa... 82 2e-042
gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-a... 75 7e-040
gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgar... 75 7e-040
gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm librar... 75 7e-040
gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA li... 72 9e-040
gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA li... 72 9e-040
gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day ger... 101 2e-039
gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green... 68 5e-038
gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. v... 75 5e-038
gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA li... 75 5e-038
gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA li... 73 1e-037
gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA li... 72 7e-035
gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. v... 72 7e-035
gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA li... 67 2e-034
gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA li... 75 1e-033
gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA li... 75 2e-031
gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day ger... 81 2e-029
gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. v... 64 8e-029
gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no... 75 2e-027
gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-a... 62 6e-027
gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA li... 72 2e-025
gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA li... 72 2e-025
gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA li... 72 2e-025
gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA li... 72 2e-025
gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA li... 75 3e-025
gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA li... 75 6e-025
gb|BQ664391.1|BQ664391 HV03B24u HV Hordeum vulgare subsp. v... 90 2e-023
gb|BQ460226.1|BQ460226 HA06N23r HA Hordeum vulgare subsp. v... 65 4e-023
gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. v... 72 1e-022
gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-a... 62 4e-021
>gb|BJ467937.1|BJ467937 BJ467937 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g13 3', mRNA sequence
Length = 703
Score = 242 bits (522), Expect(2) = 2e-079
Identities = 92/109 (84%), Positives = 100/109 (91%)
Frame = -2 / -1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGYTTVDLATIKEGAVLHYNGNMKPWLK 576
DHTLWKLGSLPPGLLAFYGLVEAL+PKWHVLGLGYTTVD TIKEGAVLHYNGN+KPWLK
Sbjct: 619 DHTLWKLGSLPPGLLAFYGLVEALNPKWHVLGLGYTTVDPDTIKEGAVLHYNGNLKPWLK 440
Query: 575 IGMEKYKSFWDNYVDYSHPLIQQCFMR*LMCINIRIFVSFMVDMFQAHA 429
IGMEKYK FWDNYVDYSHPL+Q+CFMR*LMCINIR F ++D+ Q H+
Sbjct: 439 IGMEKYKGFWDNYVDYSHPLLQRCFMR*LMCINIRTFCDLLMDVLQMHS 293
Score = 191 bits (412), Expect(2) = 9e-061
Identities = 85/108 (78%), Positives = 92/108 (85%)
Frame = +1 / +3
Query: 433 CAWNISTMKLTKILMLIHINQRMKHC*IKGCE*ST*LSQKLLYFSIPILSHGFIFPL*CS 612
C S + K+L+LIHINQRMKH * +GCE*ST*LSQK LYFSIPI SHGF FPL*C+
Sbjct: 297 CI*RTSIRRSQKVLILIHINQRMKHR*SRGCE*ST*LSQKPLYFSIPIFSHGFKFPL*CN 476
Query: 613 TAPSLIVARSTVV*PSPKTCHLGSNASTKP*KARSPGGKDPSFHRV*S 756
TAPSLIV+ STVV*PSPKTCH G +ASTKP*KARSPGGKDPSFHRV*S
Sbjct: 477 TAPSLIVSGSTVV*PSPKTCHFGFSASTKP*KARSPGGKDPSFHRV*S 620
Score = 174 bits (375), Expect(2) = 5e-055
Identities = 69/111 (62%), Positives = 90/111 (81%)
Frame = +3 / +2
Query: 429 SMCLEHIYHEAYKNSNVDTHQSTHEALLNQRV*IVHIVIPKALVLLHSNFEPWLHISIVV 608
+M L+ ++ + K+SN+DTH S HEA L QRV*I+HIV+P+A VLLH NF+PWL +SIV+
Sbjct: 293 AMHLKDVHQKVTKSSNIDTH*SAHEAPLKQRV*IIHIVVPEAFVLLHPNFQPWLQVSIVM 472
Query: 609 QYCTFLDSSKIHSCVAKSQNMPFGIQCFNQAIESKKSRW*RPKLP*SMITL 761
QYCTF +S +I SCVA+ Q+MPF +QCFNQAIESKK RW*R +LP MI++
Sbjct: 473 QYCTFFNSIRIDSCVAQPQDMPFWVQCFNQAIESKKPRW*RSELPQGMISI 625
Score = 169 bits (364), Expect(4) = 1e-053
Identities = 70/104 (67%), Positives = 80/104 (76%)
Frame = -1 / -3
Query: 777 IIGRSAM*SYSMEAWVFTTWTSCFLWLG*SIGSQMACFGTWLHNCGSCYYQGRCSTALQW 598
++ + *SY +EA +FTTW SCFLWLG*S +MAC G LHNC S YY RCS ALQW
Sbjct: 641 LLAGAQC*SYPVEARIFTTWASCFLWLG*STEPKMACLGAGLHNCRS*YY*RRCSIALQW 462
Query: 597 KYEAMAQNWNGEVQELLG*LCGLFTPFDSAVLHALIDVYQH*NF 466
K EAMA+NW+GEVQ LLG LCGLFTP S VLHALI+VYQ+*NF
Sbjct: 461 KLEAMAENWDGEVQRLLGQLCGLFTPSASTVLHALINVYQY*NF 330
Score = 152 bits (326), Expect(2) = 2e-045
Identities = 67/96 (69%), Positives = 72/96 (75%)
Frame = -3 / -2
Query: 757 VIILYGSLGLYHLDFLLSMAWLKHWIPNGMFWDLATQLWILLLSRKVQYCTTMEI*SHGS 578
+II GS LYHL FLLSMAWLKH NGM W ATQL IL+L +KVQYC TME *SHG
Sbjct: 621 LIIPCGSSDLYHLGFLLSMAWLKH*TQNGMSWGWATQLSILILLKKVQYCITMET*SHG* 442
Query: 577 KLEWRSTRAFGITMWTIHTL*FSSASCVD*CVSTLE 470
KL WRST+A G TMW IHTL F+ ASC D*CVS LE
Sbjct: 441 KLGWRSTKASGTTMWIIHTLCFNGASCAD*CVSILE 334
Score = 147 bits (316), Expect(3) = 3e-049
Identities = 66/97 (68%), Positives = 77/97 (79%)
Frame = +2 / +1
Query: 464 QKF*C*YTSINA*STAESKGVNSPHSYPKSSCTSPFQF*AMASYFHCSAVLHLP***QDP 643
+KF* *YT I+A*ST E++GVN+PHS P+S CTSP QF AMAS FHC+A+LHL ** QD
Sbjct: 328 KKF*Y*YTLISA*STVEAEGVNNPHSCPRSLCTSPSQFSAMASSFHCNAILHLL**YQDR 507
Query: 644 QLCSQVPKHAIWDPMLQPSHRKQEVQVVKTQASIEYD 754
QLCS P+HAI +LQPSHRKQE QVVK +AS YD
Sbjct: 508 QLCSPAPRHAILGSVLQPSHRKQEAQVVKIRASTGYD 618
Score = 74.4 bits (156), Expect(2) = 2e-079
Identities = 27/27 (100%), Positives = 27/27 (100%)
Frame = -3 / -1
Query: 838 FGMNVLDLVEWRNKNVTGIYHYWQERN 758
FGMNVLDLVEWRNKNVTGIYHYWQERN
Sbjct: 703 FGMNVLDLVEWRNKNVTGIYHYWQERN 623
Score = 62.5 bits (130), Expect(2) = 9e-061
Identities = 23/27 (85%), Positives = 26/27 (96%)
Frame = +2 / +2
Query: 758 IALLPIMIYACHILVPPFNQIKHVHTK 838
IALLPIMIY C+ILVPPFN+IKH+HTK
Sbjct: 623 IALLPIMIYTCNILVPPFNKIKHIHTK 703
Score = 60.2 bits (125), Expect(2) = 5e-055
Identities = 25/26 (96%), Positives = 26/26 (100%)
Frame = +3 / +3
Query: 759 LRSCQ**YMPVTFLFLHSTRSSTFIP 836
LRSCQ**Y+PVTFLFLHSTRSSTFIP
Sbjct: 624 LRSCQ**YIPVTFLFLHSTRSSTFIP 701
Score = 57.9 bits (120), Expect(3) = 3e-049
Identities = 23/27 (85%), Positives = 23/27 (85%)
Frame = +1 / +1
Query: 757 HCAPANNDICLSHSCSSIQPDQARSYQ 837
HCAPANNDI L HSCSSIQ DQA SYQ
Sbjct: 622 HCAPANNDIYL*HSCSSIQQDQAHSYQ 702
Score = 52.8 bits (109), Expect(4) = 1e-053
Identities = 23/27 (85%), Positives = 24/27 (88%)
Frame = -2 / -3
Query: 836 WYERA*SG*MEEQECDRHISLLAGAQC 756
WYE A*S *MEEQEC R+ISLLAGAQC
Sbjct: 701 WYECA*SC*MEEQECYRYISLLAGAQC 621
Score = 50.6 bits (104), Expect(2) = 2e-045
Identities = 23/27 (85%), Positives = 23/27 (85%)
Frame = -1 / -2
Query: 837 LV*TCLIWLNGGTRM*QAYIIIGRSAM 757
LV* CLI LNGGTRM Q YIIIGRSAM
Sbjct: 702 LV*MCLILLNGGTRMLQVYIIIGRSAM 622
Score = 28.6 bits (56), Expect(3) = 3e-049
Identities = 16/51 (31%), Positives = 22/51 (43%)
Frame = +3 / +1
Query: 315 CCAPSTTDAIDIH*CFVRDCNLREKPTDLTTMFPCGSWSMCLEHIYHEAYK 467
C +T +I+ C + CNL K + T MF C S E E +K
Sbjct: 178 CTLSTTHASINNCPCLI*GCNL*MKLIEFTRMFVCACSSYAFEGRPSEGHK 330
Score = 22.1 bits (42), Expect(4) = 1e-053
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -1 / -1
Query: 279 S*DCYAVGCYL 247
S*D ++VGCYL
Sbjct: 136 S*DWHSVGCYL 104
Score = 22.1 bits (42), Expect(4) = 1e-053
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = -1 / -1
Query: 468 FCKLHGRYVPSTCSNCHTETL*LNQLAFLSSYNL*QNISGCL 343
FC L + N HT T + A +SY L N+ CL
Sbjct: 331 FCDLLMDVLQMHSLNRHTRTFL*IR*ASFTSYTLRLNMDSCL 206
>gb|BJ467938.1|BJ467938 BJ467938 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags30g14 3', mRNA sequence
Length = 550
Score = 206 bits (445), Expect = 1e-052
Identities = 79/96 (82%), Positives = 87/96 (90%)
Frame = -2 / -3
Query: 716 LLAFYGLVEALDPKWHVLGLGYTTVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNY 537
LLAFYGLVEAL+PKWHVLGLGYTTVD TIKEGAVLHYNGN+KPWLKIGMEKYK FWDNY
Sbjct: 548 LLAFYGLVEALNPKWHVLGLGYTTVDPDTIKEGAVLHYNGNLKPWLKIGMEKYKGFWDNY 369
Query: 536 VDYSHPLIQQCFMR*LMCINIRIFVSFMVDMFQAHA 429
VDYSHPL+Q+CFMR*LMCINIR F ++D+ Q H+
Sbjct: 368 VDYSHPLLQRCFMR*LMCINIRTFCDLLMDVLQMHS 261
Score = 160 bits (343), Expect = 1e-038
Identities = 72/95 (75%), Positives = 79/95 (83%)
Frame = +1 / +1
Query: 433 CAWNISTMKLTKILMLIHINQRMKHC*IKGCE*ST*LSQKLLYFSIPILSHGFIFPL*CS 612
C S + K+L+LIHINQRMKH * +GCE*ST*LSQK LYFSIPI SHGF FPL*C+
Sbjct: 265 CI*RTSIRRSQKVLILIHINQRMKHR*SRGCE*ST*LSQKPLYFSIPIFSHGFKFPL*CN 444
Query: 613 TAPSLIVARSTVV*PSPKTCHLGSNASTKP*KARS 717
TAPSLIV+ STVV*PSPKTCH G +ASTKP*KARS
Sbjct: 445 TAPSLIVSGSTVV*PSPKTCHFGFSASTKP*KARS 549
Score = 156 bits (335), Expect = 2e-037
Identities = 61/96 (63%), Positives = 79/96 (82%)
Frame = +3 / +3
Query: 429 SMCLEHIYHEAYKNSNVDTHQSTHEALLNQRV*IVHIVIPKALVLLHSNFEPWLHISIVV 608
+M L+ ++ + K+SN+DTH S HEA L QRV*I+HIV+P+A VLLH NF+PWL +SIV+
Sbjct: 261 AMHLKDVHQKVTKSSNIDTH*SAHEAPLKQRV*IIHIVVPEAFVLLHPNFQPWLQVSIVM 440
Query: 609 QYCTFLDSSKIHSCVAKSQNMPFGIQCFNQAIESKK 716
QYCTF +S +I SCVA+ Q+MPF +QCFNQAIESKK
Sbjct: 441 QYCTFFNSIRIDSCVAQPQDMPFWVQCFNQAIESKK 548
Score = 147 bits (315), Expect(3) = 1e-036
Identities = 61/83 (73%), Positives = 66/83 (79%)
Frame = -1 / -2
Query: 714 SCFLWLG*SIGSQMACFGTWLHNCGSCYYQGRCSTALQWKYEAMAQNWNGEVQELLG*LC 535
SCFLWLG*S +MAC G LHNC S YY RCS ALQWK EAMA+NW+GEVQ LLG LC
Sbjct: 546 SCFLWLG*STEPKMACLGAGLHNCRS*YY*RRCSIALQWKLEAMAENWDGEVQRLLGQLC 367
Query: 534 GLFTPFDSAVLHALIDVYQH*NF 466
GLFTP S VLHALI+VYQ+*NF
Sbjct: 366 GLFTPSASTVLHALINVYQY*NF 298
Score = 137 bits (293), Expect = 1e-031
Identities = 59/82 (71%), Positives = 63/82 (76%)
Frame = -3 / -1
Query: 715 FLLSMAWLKHWIPNGMFWDLATQLWILLLSRKVQYCTTMEI*SHGSKLEWRSTRAFGITM 536
FLLSMAWLKH NGM W ATQL IL+L +KVQYC TME *SHG KL WRST+A G TM
Sbjct: 547 FLLSMAWLKH*TQNGMSWGWATQLSILILLKKVQYCITMET*SHG*KLGWRSTKASGTTM 368
Query: 535 WTIHTL*FSSASCVD*CVSTLE 470
W IHTL F+ ASC D*CVS LE
Sbjct: 367 WIIHTLCFNGASCAD*CVSILE 302
Score = 131 bits (280), Expect(2) = 1e-032
Identities = 58/84 (69%), Positives = 68/84 (80%)
Frame = +2 / +2
Query: 464 QKF*C*YTSINA*STAESKGVNSPHSYPKSSCTSPFQF*AMASYFHCSAVLHLP***QDP 643
+KF* *YT I+A*ST E++GVN+PHS P+S CTSP QF AMAS FHC+A+LHL ** QD
Sbjct: 296 KKF*Y*YTLISA*STVEAEGVNNPHSCPRSLCTSPSQFSAMASSFHCNAILHLL**YQDR 475
Query: 644 QLCSQVPKHAIWDPMLQPSHRKQE 715
QLCS P+HAI +LQPSHRKQE
Sbjct: 476 QLCSPAPRHAILGSVLQPSHRKQE 547
Score = 28.6 bits (56), Expect(2) = 1e-032
Identities = 16/51 (31%), Positives = 22/51 (43%)
Frame = +3 / +2
Query: 315 CCAPSTTDAIDIH*CFVRDCNLREKPTDLTTMFPCGSWSMCLEHIYHEAYK 467
C +T +I+ C + CNL K + T MF C S E E +K
Sbjct: 146 CTLSTTHASINNCPCLI*GCNL*MKLIEFTRMFVCACSSYAFEGRPSEGHK 298
Score = 22.1 bits (42), Expect(3) = 1e-036
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -1 / -3
Query: 279 S*DCYAVGCYL 247
S*D ++VGCYL
Sbjct: 104 S*DWHSVGCYL 72
Score = 22.1 bits (42), Expect(3) = 1e-036
Identities = 14/42 (33%), Positives = 18/42 (42%)
Frame = -1 / -3
Query: 468 FCKLHGRYVPSTCSNCHTETL*LNQLAFLSSYNL*QNISGCL 343
FC L + N HT T + A +SY L N+ CL
Sbjct: 299 FCDLLMDVLQMHSLNRHTRTFL*IR*ASFTSYTLRLNMDSCL 174
>gb|BG417087.1|BG417087 HVSMEk0016D06f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0016D06f, mRNA sequence
Length = 490
Score = 82.2 bits (173), Expect(3) = 2e-042
Identities = 27/54 (50%), Positives = 40/54 (74%)
Frame = -2 / +3
Query: 644 VDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQCFMR*LMC 483
+D I+ AV+HYNGNMKPWLK+ + +YKS W+ +V++SHP I++C * +C
Sbjct: 249 IDDRLIESAAVVHYNGNMKPWLKLAIHRYKSIWERHVNFSHPHIRECMFH*AVC 410
Score = 65.7 bits (137), Expect(3) = 2e-042
Identities = 23/35 (65%), Positives = 28/35 (80%)
Frame = -2 / +3
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LW+ G+LP GLL FYGL+E LD +WH+LGLGY
Sbjct: 135 DQLLWRTGTLPAGLLTFYGLMEPLDRRWHLLGLGY 239
Score = 62.9 bits (131), Expect(3) = 2e-042
Identities = 19/29 (65%), Positives = 23/29 (79%)
Frame = -3 / +3
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WAFGMN+ DL+ WR N T +YHYWQE+N
Sbjct: 45 WAFGMNIFDLIAWRKANATSLYHYWQEQN 131
>gb|BG345023.1|BG345023 HVSMEg0018F09f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0018F09f, mRNA sequence
Length = 856
Score = 75.3 bits (158), Expect(3) = 7e-040
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / +1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++D + I AV HYNGNMKPWL++ M KY+ +W Y+ Y HP I+ C
Sbjct: 211 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 354
Score = 67.0 bits (140), Expect(3) = 7e-040
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / +1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 100 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 204
Score = 60.2 bits (125), Expect(3) = 7e-040
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WA+GMN+ DL +W+NK++TGIYH WQ N
Sbjct: 10 WAYGMNIFDLKQWKNKDITGIYHKWQNMN 96
>gb|CK123727.1|CK123727 BES1824105d06 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010D065 5-PRIME, mRNA sequence
Length = 786
Score = 75.3 bits (158), Expect(3) = 7e-040
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / +1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++D + I AV HYNGNMKPWL++ M KY+ +W Y+ Y HP I+ C
Sbjct: 367 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 510
Score = 67.0 bits (140), Expect(3) = 7e-040
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / +1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 256 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 360
Score = 60.2 bits (125), Expect(3) = 7e-040
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WA+GMN+ DL +W+NK++TGIYH WQ N
Sbjct: 166 WAYGMNIFDLKQWKNKDITGIYHKWQNMN 252
>gb|CV055942.1|CV055942 BNEL12B7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL12B7 5' similar to 68 kDa
protein, mRNA sequence
Length = 616
Score = 75.3 bits (158), Expect(3) = 7e-040
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / +1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++D + I AV HYNGNMKPWL++ M KY+ +W Y+ Y HP I+ C
Sbjct: 328 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 471
Score = 67.0 bits (140), Expect(3) = 7e-040
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / +1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 217 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 321
Score = 60.2 bits (125), Expect(3) = 7e-040
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WA+GMN+ DL +W+NK++TGIYH WQ N
Sbjct: 127 WAYGMNIFDLKQWKNKDITGIYHKWQNMN 213
>gb|BJ447514.1|BJ447514 BJ447514 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 5', mRNA sequence
Length = 630
Score = 71.6 bits (150), Expect(3) = 9e-040
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / +2
Query: 644 VDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQCF 501
VD+A I+ AV+HYNGN KPWL + + KYK++W YVD I+ C+
Sbjct: 341 VDIAEIENAAVVHYNGNYKPWLDLAISKYKAYWSKYVDVDSSHIRHCY 484
Score = 66.6 bits (139), Expect(3) = 9e-040
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / +2
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 227 DRKLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGY 331
Score = 63.8 bits (133), Expect(3) = 9e-040
Identities = 20/29 (68%), Positives = 24/29 (82%)
Frame = -3 / +2
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WAFGMN+ DL EW+ +N+TGIYHYWQ N
Sbjct: 137 WAFGMNMFDLKEWKKRNITGIYHYWQNLN 223
>gb|BJ455274.1|BJ455274 BJ455274 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak16a24 3', mRNA sequence
Length = 565
Score = 71.6 bits (150), Expect(3) = 9e-040
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / -1
Query: 644 VDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQCF 501
VD+A I+ AV+HYNGN KPWL + + KYK++W YVD I+ C+
Sbjct: 229 VDIAEIENAAVVHYNGNYKPWLDLAISKYKAYWSKYVDVDSSHIRHCY 86
Score = 66.6 bits (139), Expect(3) = 9e-040
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / -1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 343 DRKLWKLGTLPPGLITFYNLTHPLDHTWHVLGLGY 239
Score = 63.8 bits (133), Expect(3) = 9e-040
Identities = 20/29 (68%), Positives = 24/29 (82%)
Frame = -3 / -1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WAFGMN+ DL EW+ +N+TGIYHYWQ N
Sbjct: 433 WAFGMNMFDLKEWKKRNITGIYHYWQNLN 347
>gb|BM443862.2|BM443862 EBem09_SQ001_M07_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ001_M07 5', mRNA sequence
Length = 512
Score = 101 bits (216), Expect(2) = 2e-039
Identities = 40/43 (93%), Positives = 41/43 (95%)
Frame = -2 / +3
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGYTTVDLATI 627
DHTLWKLGSLPPGLLAFYGLVEAL+PKWHVLGLGYTTVD TI
Sbjct: 384 DHTLWKLGSLPPGLLAFYGLVEALNPKWHVLGLGYTTVDPDTI 512
Score = 89.0 bits (188), Expect(2) = 2e-031
Identities = 38/43 (88%), Positives = 40/43 (93%)
Frame = +1 / -2
Query: 628 IVARSTVV*PSPKTCHLGSNASTKP*KARSPGGKDPSFHRV*S 756
IV+ STVV*PSPKTCH G +ASTKP*KARSPGGKDPSFHRV*S
Sbjct: 511 IVSGSTVV*PSPKTCHFGFSASTKP*KARSPGGKDPSFHRV*S 383
Score = 81.3 bits (171), Expect(2) = 2e-039
Identities = 29/29 (100%), Positives = 29/29 (100%)
Frame = -3 / +3
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WAFGMNVLDLVEWRNKNVTGIYHYWQERN
Sbjct: 294 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 380
Score = 74.4 bits (156), Expect(2) = 1e-024
Identities = 29/50 (58%), Positives = 35/50 (70%)
Frame = -1 / +2
Query: 777 IIGRSAM*SYSMEAWVFTTWTSCFLWLG*SIGSQMACFGTWLHNCGSCYY 628
++ + *SY +EA +FTTW SCFLWLG*S +MAC G LHNC S YY
Sbjct: 362 LLAGAQC*SYPVEARIFTTWASCFLWLG*STEPKMACLGAGLHNCRS*YY 511
Score = 72.5 bits (152), Expect(2) = 9e-026
Identities = 29/45 (64%), Positives = 37/45 (82%)
Frame = +3 / -1
Query: 627 DSSKIHSCVAKSQNMPFGIQCFNQAIESKKSRW*RPKLP*SMITL 761
+S +I SCVA+ Q+MPF +QCFNQAIESKK RW*R +LP MI++
Sbjct: 512 NSIRIDSCVAQPQDMPFWVQCFNQAIESKKPRW*RSELPQGMISI 378
Score = 67.0 bits (140), Expect(2) = 2e-031
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +3 / -2
Query: 759 LRSCQ**YMPVTFLFLHSTRSSTFIPNAQ 845
LRSCQ**Y+PVTFLFLHSTRSSTFIPNAQ
Sbjct: 379 LRSCQ**YIPVTFLFLHSTRSSTFIPNAQ 293
Score = 65.2 bits (136), Expect(2) = 5e-023
Identities = 27/40 (67%), Positives = 30/40 (75%)
Frame = +2 / -3
Query: 635 QDPQLCSQVPKHAIWDPMLQPSHRKQEVQVVKTQASIEYD 754
QD QLCS P+HAI +LQPSHRKQE QVVK +AS YD
Sbjct: 504 QDRQLCSPAPRHAILGSVLQPSHRKQEAQVVKIRASTGYD 385
Score = 64.3 bits (134), Expect(2) = 9e-026
Identities = 24/29 (82%), Positives = 27/29 (93%)
Frame = +2 / -1
Query: 758 IALLPIMIYACHILVPPFNQIKHVHTKCP 844
IALLPIMIY C+ILVPPFN+IKH+HTK P
Sbjct: 380 IALLPIMIYTCNILVPPFNKIKHIHTKRP 294
Score = 62.5 bits (130), Expect(2) = 5e-023
Identities = 25/30 (83%), Positives = 25/30 (83%)
Frame = +1 / -3
Query: 757 HCAPANNDICLSHSCSSIQPDQARSYQMPS 846
HCAPANNDI L HSCSSIQ DQA SYQ PS
Sbjct: 381 HCAPANNDIYL*HSCSSIQQDQAHSYQTPS 292
Score = 58.8 bits (122), Expect(2) = 1e-024
Identities = 25/30 (83%), Positives = 27/30 (90%)
Frame = -2 / +2
Query: 845 LGIWYERA*SG*MEEQECDRHISLLAGAQC 756
LG+WYE A*S *MEEQEC R+ISLLAGAQC
Sbjct: 293 LGVWYECA*SC*MEEQECYRYISLLAGAQC 382
>gb|BI956176.1|BI956176 HVSMEm0025O15f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0025O15f, mRNA sequence
Length = 674
Score = 67.5 bits (141), Expect(3) = 5e-038
Identities = 24/46 (52%), Positives = 33/46 (71%)
Frame = -2 / +1
Query: 644 VDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQ 507
+D I+ AV+HYNGNMKPWLK+ + YKS W V++SHP I++
Sbjct: 427 IDDRLIESAAVVHYNGNMKPWLKLAIHPYKSIWGADVNFSHPHIRE 564
Score = 65.7 bits (137), Expect(3) = 5e-038
Identities = 23/35 (65%), Positives = 28/35 (80%)
Frame = -2 / +1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LW+ G+LP GLL FYGL+E LD +WH+LGLGY
Sbjct: 313 DQLLWRTGTLPAGLLTFYGLMEPLDRRWHLLGLGY 417
Score = 62.9 bits (131), Expect(3) = 5e-038
Identities = 19/29 (65%), Positives = 23/29 (79%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WAFGMN+ DL+ WR N T +YHYWQE+N
Sbjct: 223 WAFGMNIFDLIAWRKANATSLYHYWQEQN 309
>gb|CA032293.1|CA032293 HX12J15r HX Hordeum vulgare subsp. vulgare cDNA clone HX12J15
5-PRIME, mRNA sequence
Length = 653
Score = 74.8 bits (157), Expect(3) = 5e-038
Identities = 21/42 (50%), Positives = 34/42 (80%)
Frame = -2 / -3
Query: 629 IKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ AV+HYNGN+KPWL+IG+ K++S+W Y+DY P +++C
Sbjct: 306 LERAAVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLREC 181
Score = 62.0 bits (129), Expect(3) = 5e-038
Identities = 22/38 (57%), Positives = 27/38 (71%)
Frame = -3 / -3
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WAFGMNV DL EWR +N+T +YH+WQ+ N L LG
Sbjct: 525 WAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLG 412
Score = 59.3 bits (123), Expect(3) = 5e-038
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = -2 / -3
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ F+ LD WHVLGLGY
Sbjct: 435 DGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGY 331
>gb|AV922076.1|AV922076 AV922076 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 3', mRNA sequence
Length = 643
Score = 74.8 bits (157), Expect(3) = 5e-038
Identities = 21/42 (50%), Positives = 34/42 (80%)
Frame = -2 / -3
Query: 629 IKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ AV+HYNGN+KPWL+IG+ K++S+W Y+DY P +++C
Sbjct: 293 LERAAVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLREC 168
Score = 62.0 bits (129), Expect(3) = 5e-038
Identities = 22/38 (57%), Positives = 27/38 (71%)
Frame = -3 / -3
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WAFGMNV DL EWR +N+T +YH+WQ+ N L LG
Sbjct: 512 WAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLG 399
Score = 59.3 bits (123), Expect(3) = 5e-038
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = -2 / -3
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ F+ LD WHVLGLGY
Sbjct: 422 DGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGY 318
>gb|AV916779.1|AV916779 AV916779 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j21 5', mRNA sequence
Length = 612
Score = 73.5 bits (154), Expect(3) = 1e-037
Identities = 21/38 (55%), Positives = 32/38 (84%)
Frame = -2 / +3
Query: 617 AVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
AV+HYNGN+KPWL+IG+ K++S+W Y+DY P +++C
Sbjct: 444 AVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLREC 557
Score = 62.0 bits (129), Expect(3) = 1e-037
Identities = 22/38 (57%), Positives = 27/38 (71%)
Frame = -3 / +2
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WAFGMNV DL EWR +N+T +YH+WQ+ N L LG
Sbjct: 212 WAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLG 325
Score = 59.3 bits (123), Expect(3) = 1e-037
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = -2 / +2
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ F+ LD WHVLGLGY
Sbjct: 302 DGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGY 406
>gb|BJ549963.1|BJ549963 BJ549963 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags25a14 3', mRNA sequence
Length = 625
Score = 72.1 bits (151), Expect(3) = 7e-035
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / -3
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 392 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 249
Score = 63.4 bits (132), Expect(3) = 7e-035
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -2 / -3
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 494 LWKLGTLPPGLITFYSTTKPLDKSWHVLGLGY 399
Score = 50.1 bits (103), Expect(3) = 7e-035
Identities = 19/38 (50%), Positives = 22/38 (57%)
Frame = -3 / -3
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WA+GMN DL WR + T YHYWQ +N L LG
Sbjct: 593 WAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLG 480
>gb|CA032787.1|CA032787 HX14D07r HX Hordeum vulgare subsp. vulgare cDNA clone HX14D07
5-PRIME, mRNA sequence
Length = 529
Score = 72.1 bits (151), Expect(3) = 7e-035
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / +2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 386 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 529
Score = 63.4 bits (132), Expect(3) = 7e-035
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -2 / +2
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 284 LWKLGTLPPGLITFYSTTKPLDKSWHVLGLGY 379
Score = 50.1 bits (103), Expect(3) = 7e-035
Identities = 19/38 (50%), Positives = 22/38 (57%)
Frame = -3 / +2
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WA+GMN DL WR + T YHYWQ +N L LG
Sbjct: 185 WAYGMNFFDLNSWRREKSTEQYHYWQTQNENRLLWKLG 298
>gb|BJ465104.1|BJ465104 BJ465104 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 5', mRNA sequence
Length = 598
Score = 67.0 bits (140), Expect(3) = 2e-034
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / +2
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 383 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 487
Score = 60.2 bits (125), Expect(3) = 2e-034
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = -3 / +2
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WA+GMN+ DL +W+NK++TGIYH WQ N
Sbjct: 293 WAYGMNIFDLKQWKNKDITGIYHKWQNMN 379
Score = 56.5 bits (117), Expect(3) = 2e-034
Identities = 18/34 (52%), Positives = 25/34 (73%)
Frame = -2 / +2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFW 546
++D + I AV HYNGNMKPWL++ M KY+ +W
Sbjct: 494 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYW 595
>gb|AV837092.1|AV837092 AV837092 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd23f10, mRNA
sequence
Length = 643
Score = 75.3 bits (158), Expect(3) = 1e-033
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / -2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++D + I AV HYNGNMKPWL++ M KY+ +W Y+ Y HP I+ C
Sbjct: 471 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 328
Score = 67.0 bits (140), Expect(3) = 1e-033
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / -2
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 582 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 478
Score = 39.1 bits (79), Expect(3) = 1e-033
Identities = 11/18 (61%), Positives = 15/18 (83%)
Frame = -3 / -2
Query: 811 EWRNKNVTGIYHYWQERN 758
+W+NK++TGIYH WQ N
Sbjct: 639 QWKNKDITGIYHKWQNMN 586
>gb|BJ468235.1|BJ468235 BJ468235 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37m09 3', mRNA sequence
Length = 634
Score = 75.3 bits (158), Expect(3) = 2e-031
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / -1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++D + I AV HYNGNMKPWL++ M KY+ +W Y+ Y HP I+ C
Sbjct: 469 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 326
Score = 67.0 bits (140), Expect(3) = 2e-031
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / -1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 580 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 476
Score = 31.8 bits (63), Expect(3) = 2e-031
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = -3 / -1
Query: 805 RNKNVTGIYHYWQERN 758
R K++TGIYH WQ N
Sbjct: 631 RTKDITGIYHKWQNMN 584
>gb|BM444115.1|BM444115 EBem09_SQ004_D08_R embryo, 1 Day germination, no treatment, cv
Optic, EBem09 Hordeum vulgare subsp. vulgare cDNA clone
EBem09_SQ004_D08 5', mRNA sequence
Length = 464
Score = 81.3 bits (171), Expect(2) = 2e-029
Identities = 29/29 (100%), Positives = 29/29 (100%)
Frame = -3 / +3
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WAFGMNVLDLVEWRNKNVTGIYHYWQERN
Sbjct: 294 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 380
Score = 68.0 bits (142), Expect(2) = 2e-029
Identities = 26/27 (96%), Positives = 27/27 (100%)
Frame = -2 / +3
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPK 675
DHTLWKLGSLPPGLLAFYGLVEAL+PK
Sbjct: 384 DHTLWKLGSLPPGLLAFYGLVEALNPK 464
Score = 67.0 bits (140), Expect(2) = 6e-022
Identities = 28/29 (96%), Positives = 29/29 (100%)
Frame = +3 / -2
Query: 759 LRSCQ**YMPVTFLFLHSTRSSTFIPNAQ 845
LRSCQ**Y+PVTFLFLHSTRSSTFIPNAQ
Sbjct: 379 LRSCQ**YIPVTFLFLHSTRSSTFIPNAQ 293
Score = 57.0 bits (118), Expect(2) = 6e-022
Identities = 24/26 (92%), Positives = 25/26 (96%)
Frame = +1 / -2
Query: 679 GSNASTKP*KARSPGGKDPSFHRV*S 756
G +ASTKP*KARSPGGKDPSFHRV*S
Sbjct: 460 GFSASTKP*KARSPGGKDPSFHRV*S 383
>gb|BU969156.1|BU969156 HB10L09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10L09
5-PRIME, mRNA sequence
Length = 684
Score = 64.3 bits (134), Expect(3) = 8e-029
Identities = 23/32 (71%), Positives = 25/32 (78%)
Frame = -2 / +3
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 516 LWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 611
Score = 59.3 bits (123), Expect(3) = 8e-029
Identities = 21/38 (55%), Positives = 26/38 (68%)
Frame = -3 / +3
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WA+GMN+ DL EW+ K++TGIYH WQ N L LG
Sbjct: 417 WAYGMNMFDLEEWKKKDITGIYHKWQNMNENRLLWKLG 530
Score = 41.4 bits (84), Expect(3) = 8e-029
Identities = 15/22 (68%), Positives = 17/22 (77%)
Frame = -2 / +3
Query: 647 TVDLATIKEGAVLHYNGNMKPW 582
TV+ A I AV+HYNGNMKPW
Sbjct: 618 TVEHAEIDTAAVIHYNGNMKPW 683
>gb|BM372595.2|BM372595 EBpi05_SQ003_G13_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_G13 5', mRNA sequence
Length = 523
Score = 75.3 bits (158), Expect(2) = 2e-027
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / +2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++D + I AV HYNGNMKPWL++ M KY+ +W Y+ Y HP I+ C
Sbjct: 131 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 274
Score = 67.0 bits (140), Expect(2) = 2e-027
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / +2
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 20 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 124
>gb|BG343589.1|BG343589 HVSMEg0006H13f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006H13f, mRNA sequence
Length = 1018
Score = 62.0 bits (129), Expect(3) = 6e-027
Identities = 22/38 (57%), Positives = 27/38 (71%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WAFGMNV DL EWR +N+T +YH+WQ+ N L LG
Sbjct: 379 WAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLG 492
Score = 59.3 bits (123), Expect(3) = 6e-027
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = -2 / +1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ F+ LD WHVLGLGY
Sbjct: 469 DGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGY 573
Score = 37.3 bits (75), Expect(3) = 6e-027
Identities = 10/26 (38%), Positives = 20/26 (76%)
Frame = -2 / +1
Query: 629 IKEGAVLHYNGNMKPWLKIGMEKYKS 552
++ AV+HY G++KPWL+ G+ +++
Sbjct: 598 LERAAVIHYXGHLKPWLEXGLPSFEA 675
>gb|BJ544949.1|BJ544949 BJ544949 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 5', mRNA sequence
Length = 589
Score = 72.1 bits (151), Expect(2) = 2e-025
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / +2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 140 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 283
Score = 63.4 bits (132), Expect(2) = 2e-025
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -2 / +2
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 38 LWKLGTLPPGLITFYSTTKPLDKSWHVLGLGY 133
>gb|AV909171.1|AV909171 AV909171 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 5', mRNA sequence
Length = 586
Score = 72.1 bits (151), Expect(2) = 2e-025
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / +2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 137 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 280
Score = 63.4 bits (132), Expect(2) = 2e-025
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -2 / +2
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 35 LWKLGTLPPGLITFYSTTKPLDKSWHVLGLGY 130
>gb|AV911054.1|AV911054 AV911054 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11o03 3', mRNA sequence
Length = 527
Score = 72.1 bits (151), Expect(2) = 2e-025
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / -2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 391 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 248
Score = 63.4 bits (132), Expect(2) = 2e-025
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -2 / -2
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 493 LWKLGTLPPGLITFYSTTKPLDKSWHVLGLGY 398
>gb|BJ546433.1|BJ546433 BJ546433 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41d13 3', mRNA sequence
Length = 525
Score = 72.1 bits (151), Expect(2) = 2e-025
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / -1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 390 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 247
Score = 63.4 bits (132), Expect(2) = 2e-025
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -2 / -1
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 492 LWKLGTLPPGLITFYSTTKPLDKSWHVLGLGY 397
>gb|AV931472.1|AV931472 AV931472 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd19o15 3', mRNA sequence
Length = 635
Score = 74.8 bits (157), Expect(3) = 3e-025
Identities = 21/42 (50%), Positives = 34/42 (80%)
Frame = -2 / -2
Query: 629 IKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ AV+HYNGN+KPWL+IG+ K++S+W Y+DY P +++C
Sbjct: 478 LERAAVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLREC 353
Score = 59.3 bits (123), Expect(3) = 3e-025
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = -2 / -2
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ F+ LD WHVLGLGY
Sbjct: 607 DGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGY 503
Score = 21.7 bits (41), Expect(3) = 3e-025
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = -3 / -2
Query: 781 YHYWQERNVIILYGSLG 731
YH+WQ+ N L LG
Sbjct: 634 YHFWQKLNEDGLLWKLG 584
>gb|AV930935.1|AV930935 AV930935 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd25o11 3', mRNA sequence
Length = 495
Score = 74.8 bits (157), Expect(2) = 6e-025
Identities = 21/42 (50%), Positives = 34/42 (80%)
Frame = -2 / -1
Query: 629 IKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ AV+HYNGN+KPWL+IG+ K++S+W Y+DY P +++C
Sbjct: 360 LERAAVIHYNGNLKPWLEIGLPKFRSYWSKYLDYDQPFLREC 235
Score = 59.3 bits (123), Expect(2) = 6e-025
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = -2 / -1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ F+ LD WHVLGLGY
Sbjct: 489 DGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGY 385
>gb|BQ664391.1|BQ664391 HV03B24u HV Hordeum vulgare subsp. vulgare cDNA clone HV03B24
3-PRIME, mRNA sequence
Length = 503
Score = 90.4 bits (191), Expect(4) = 2e-023
Identities = 32/45 (71%), Positives = 38/45 (84%)
Frame = -2 / -1
Query: 563 KYKSFWDNYVDYSHPLIQQCFMR*LMCINIRIFVSFMVDMFQAHA 429
KYK FWDNYVDYSHPL+Q+CFMR*LMCINIR F ++D+ Q H+
Sbjct: 503 KYKGFWDNYVDYSHPLLQRCFMR*LMCINIRTFCDLLMDVLQMHS 369
Score = 28.6 bits (56), Expect(4) = 2e-023
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = -1 / -3
Query: 138 FKLLMCYNKFCVTY 97
FKLL CYNKF TY
Sbjct: 75 FKLLRCYNKF*DTY 34
Score = 24.4 bits (47), Expect(4) = 2e-023
Identities = 8/13 (61%), Positives = 9/13 (69%)
Frame = -2 / -1
Query: 218 CDHRCNFSIFYFL 180
C H+CNF F FL
Sbjct: 149 CSHQCNFFHFDFL 111
Score = 22.1 bits (42), Expect(4) = 2e-023
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -1 / -1
Query: 279 S*DCYAVGCYL 247
S*D ++VGCYL
Sbjct: 212 S*DWHSVGCYL 180
>gb|BQ460226.1|BQ460226 HA06N23r HA Hordeum vulgare subsp. vulgare cDNA clone HA06N23
5-PRIME, mRNA sequence
Length = 567
Score = 65.2 bits (136), Expect(2) = 4e-023
Identities = 23/39 (58%), Positives = 28/39 (71%)
Frame = -2 / +2
Query: 767 GAQCDHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
G + TLWK G+LPP L+AF G V +DP WH+LGLGY
Sbjct: 2 GTRAGLTLWKFGTLPPALIAFRGHVHGIDPSWHMLGLGY 118
Score = 62.9 bits (131), Expect(2) = 4e-023
Identities = 20/48 (41%), Positives = 32/48 (66%)
Frame = -2 / +2
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
+ D+ ++K+ AV+HYNG KPWL I + + FW +V+YS+ I+ C
Sbjct: 125 STDIESVKKAAVVHYNGQCKPWLDIAFKNLQPFWTKHVNYSNDFIRNC 268
>gb|CB881764.1|CB881764 HM10N01w HM Hordeum vulgare subsp. vulgare cDNA clone HM10N01
3-PRIME, mRNA sequence
Length = 535
Score = 72.1 bits (151), Expect(2) = 1e-022
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / -3
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 440 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRQLWTKYVDYDDSFIRQC 297
Score = 54.2 bits (112), Expect(2) = 1e-022
Identities = 19/29 (65%), Positives = 22/29 (75%)
Frame = -2 / -3
Query: 737 LGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 533 LGTLPPGLITFYSTTKPLDKSWHVLGLGY 447
>gb|BE060385.2|BE060385 HVSMEg0012C17f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0012C17f, mRNA sequence
Length = 785
Score = 62.0 bits (129), Expect(2) = 4e-021
Identities = 22/38 (57%), Positives = 27/38 (71%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WAFGMNV DL EWR +N+T +YH+WQ+ N L LG
Sbjct: 379 WAFGMNVFDLAEWRRQNITELYHFWQKLNEDGLLWKLG 492
Score = 59.3 bits (123), Expect(2) = 4e-021
Identities = 22/35 (62%), Positives = 25/35 (71%)
Frame = -2 / +1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ F+ LD WHVLGLGY
Sbjct: 469 DGLLWKLGTLPPGLVTFWNKTFPLDRSWHVLGLGY 573
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 402,694,043
Number of Sequences: 312970
Number of extensions: 6156545
Number of successful extensions: 36620
Number of sequences better than 1.0e-020: 41
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 9641756231
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)