TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2949674.2.1
(658 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV917194.1|AV917194 AV917194 K. Sato unpublished cDNA li... 149 1e-035
gb|BQ458826.1|BQ458826 HA04B17r HA Hordeum vulgare subsp. v... 149 1e-035
gb|BQ656545.1|BQ656545 HA04B17u HA Hordeum vulgare subsp. v... 149 1e-035
gb|BU985820.1|BU985820 HF08L16r HF Hordeum vulgare subsp. v... 149 1e-035
gb|BU995569.1|BU995569 HM10K10r HM Hordeum vulgare subsp. v... 149 1e-035
gb|CB879942.1|CB879942 HM05A07w HM Hordeum vulgare subsp. v... 149 1e-035
gb|CB881728.1|CB881728 HM10L09w HM Hordeum vulgare subsp. v... 149 1e-035
gb|BM099601.2|BM099601 EBes01_SQ003_L13_R embryo sac, 4-6 D... 149 1e-035
gb|AJ462863.1|AJ462863 AJ462863 S00002 Hordeum vulgare subs... 128 3e-029
>gb|AV917194.1|AV917194 AV917194 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags8k04 5', mRNA sequence
Length = 515
Score = 149 bits (321), Expect = 1e-035
Identities = 51/74 (68%), Positives = 56/74 (75%)
Frame = +2 / +2
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 98 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 277
Query: 488 NNWKTKRGGPKCP* 529
NNWKTK GGPKCP*
Sbjct: 278 NNWKTKEGGPKCP* 319
>gb|BQ458826.1|BQ458826 HA04B17r HA Hordeum vulgare subsp. vulgare cDNA clone HA04B17
5-PRIME, mRNA sequence
Length = 514
Score = 149 bits (321), Expect = 1e-035
Identities = 51/74 (68%), Positives = 56/74 (75%)
Frame = +2 / +3
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 141 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 320
Query: 488 NNWKTKRGGPKCP* 529
NNWKTK GGPKCP*
Sbjct: 321 NNWKTKEGGPKCP* 362
>gb|BQ656545.1|BQ656545 HA04B17u HA Hordeum vulgare subsp. vulgare cDNA clone HA04B17
3-PRIME, mRNA sequence
Length = 515
Score = 149 bits (321), Expect = 1e-035
Identities = 51/74 (68%), Positives = 56/74 (75%)
Frame = +2 / -1
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 374 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 195
Query: 488 NNWKTKRGGPKCP* 529
NNWKTK GGPKCP*
Sbjct: 194 NNWKTKEGGPKCP* 153
>gb|BU985820.1|BU985820 HF08L16r HF Hordeum vulgare subsp. vulgare cDNA clone HF08L16
5-PRIME, mRNA sequence
Length = 517
Score = 149 bits (321), Expect = 1e-035
Identities = 51/74 (68%), Positives = 56/74 (75%)
Frame = +2 / +3
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 147 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 326
Query: 488 NNWKTKRGGPKCP* 529
NNWKTK GGPKCP*
Sbjct: 327 NNWKTKEGGPKCP* 368
>gb|BU995569.1|BU995569 HM10K10r HM Hordeum vulgare subsp. vulgare cDNA clone HM10K10
5-PRIME, mRNA sequence
Length = 587
Score = 149 bits (321), Expect = 1e-035
Identities = 51/74 (68%), Positives = 56/74 (75%)
Frame = +2 / +1
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 124 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 303
Query: 488 NNWKTKRGGPKCP* 529
NNWKTK GGPKCP*
Sbjct: 304 NNWKTKEGGPKCP* 345
>gb|CB879942.1|CB879942 HM05A07w HM Hordeum vulgare subsp. vulgare cDNA clone HM05A07
3-PRIME, mRNA sequence
Length = 589
Score = 149 bits (321), Expect = 1e-035
Identities = 51/74 (68%), Positives = 56/74 (75%)
Frame = +2 / -2
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 465 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 286
Query: 488 NNWKTKRGGPKCP* 529
NNWKTK GGPKCP*
Sbjct: 285 NNWKTKEGGPKCP* 244
>gb|CB881728.1|CB881728 HM10L09w HM Hordeum vulgare subsp. vulgare cDNA clone HM10L09
3-PRIME, mRNA sequence
Length = 602
Score = 149 bits (321), Expect = 1e-035
Identities = 51/74 (68%), Positives = 56/74 (75%)
Frame = +2 / -1
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 464 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 285
Query: 488 NNWKTKRGGPKCP* 529
NNWKTK GGPKCP*
Sbjct: 284 NNWKTKEGGPKCP* 243
>gb|BM099601.2|BM099601 EBes01_SQ003_L13_R embryo sac, 4-6 DPA, no treatment, cv Optic,
EBes01 Hordeum vulgare subsp. vulgare cDNA clone
EBes01_SQ003_L13 5', mRNA sequence
Length = 362
Score = 149 bits (320), Expect = 1e-035
Identities = 50/73 (68%), Positives = 55/73 (75%)
Frame = +2 / +2
Query: 308 GGVTPGTGSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCY 487
GG G G L+P EC +C+ RCS T Y+K CL +C KCCA CLCVPPGTYGNK CPCY
Sbjct: 143 GGGGGGGGKLKPWECSSKCSSRCSGTQYKKACLTYCNKCCATCLCVPPGTYGNKGACPCY 322
Query: 488 NNWKTKRGGPKCP 526
NNWKTK GGPKCP
Sbjct: 323 NNWKTKEGGPKCP 361
>gb|AJ462863.1|AJ462863 AJ462863 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200057E02F1, mRNA sequence
Length = 420
Score = 128 bits (274), Expect = 3e-029
Identities = 45/67 (67%), Positives = 50/67 (74%)
Frame = +2 / +3
Query: 329 GSLQPHECGGRCAERCSATAYQKPCLFFCRKCCAACLCVPPGTYGNKNTCPCYNNWKTKR 508
GSL+ ++C C RCS T Y+KPCLFFC KCC CLCVP G YG+K CPCYNNWKTK
Sbjct: 6 GSLKIYQCKPVCEYRCSDTKYRKPCLFFCNKCCNTCLCVPSGFYGHKYECPCYNNWKTKE 185
Query: 509 GGPKCP* 529
G PKCP*
Sbjct: 186 GRPKCP* 206
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 296,128,077
Number of Sequences: 312970
Number of extensions: 5579875
Number of successful extensions: 93914
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7083590403
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)