TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2921719.2.1
(779 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ463481.1|BQ463481 HI05G14r HI Hordeum vulgare subsp. v... 188 3e-048
gb|CA026803.1|CA026803 HZ57A06r HZ Hordeum vulgare subsp. v... 188 3e-048
gb|BQ760103.1|BQ760103 EBpi07_SQ003_P02_R pistil, 12 DPA, n... 188 3e-048
gb|CA017843.1|CA017843 HV06H11r HV Hordeum vulgare subsp. v... 188 4e-047
gb|CV060542.1|CV060542 BNEL59c5 Barley EST endosperm librar... 187 8e-047
gb|CV053867.1|CV053867 BNEL104a7 Barley EST endosperm libra... 185 3e-046
gb|BM377309.2|BM377309 EBem05_SQ004_L24_R embryo, 14 DPA, n... 172 1e-043
gb|CV054722.1|CV054722 BNEL112e9 Barley EST endosperm libra... 151 1e-041
gb|BQ756119.1|BQ756119 EBem05_SQ001_N02_R embryo, 14 DPA, n... 165 3e-040
gb|BQ660776.1|BQ660776 HI05G14u HI Hordeum vulgare subsp. v... 90 6e-022
>gb|BQ463481.1|BQ463481 HI05G14r HI Hordeum vulgare subsp. vulgare cDNA clone HI05G14
5-PRIME, mRNA sequence
Length = 454
Score = 188 bits (404), Expect(2) = 3e-048
Identities = 71/77 (92%), Positives = 77/77 (100%)
Frame = +2 / +2
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A+VDMGQIQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQQSRGWVHYA
Sbjct: 107 AVVDMGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYA 286
Query: 266 IHRPEPHIMLFRRPINY 316
+HRPEPHIMLFRRP+NY
Sbjct: 287 VHRPEPHIMLFRRPLNY 337
Score = 173 bits (372), Expect = 1e-042
Identities = 70/79 (88%), Positives = 71/79 (89%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRRNSMMCGSGRW A*CTHPRLCCTP ARHSFS RRFLGRSLATSGGRTTCLYS
Sbjct: 339 W*LSGRRNSMMCGSGRWTA*CTHPRLCCTPSARHSFSASRRFLGRSLATSGGRTTCLYSK 160
Query: 138 VSSKYFSEYWI*PMSTNAS 82
VSSKYFSEYWI PMST A+
Sbjct: 159 VSSKYFSEYWIWPMSTTAA 103
Score = 24.0 bits (46), Expect(2) = 3e-048
Identities = 5/7 (71%), Positives = 7/7 (100%)
Frame = +3 / +3
Query: 81 EKRWWTW 101
++RWWTW
Sbjct: 102 KRRWWTW 122
>gb|CA026803.1|CA026803 HZ57A06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ57A06
5-PRIME, mRNA sequence
Length = 435
Score = 188 bits (404), Expect(2) = 3e-048
Identities = 71/77 (92%), Positives = 77/77 (100%)
Frame = +2 / +2
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A+VDMGQIQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQQSRGWVHYA
Sbjct: 86 AVVDMGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYA 265
Query: 266 IHRPEPHIMLFRRPINY 316
+HRPEPHIMLFRRP+NY
Sbjct: 266 VHRPEPHIMLFRRPLNY 316
Score = 173 bits (372), Expect = 1e-042
Identities = 70/79 (88%), Positives = 71/79 (89%)
Frame = -3 / -1
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRRNSMMCGSGRW A*CTHPRLCCTP ARHSFS RRFLGRSLATSGGRTTCLYS
Sbjct: 318 W*LSGRRNSMMCGSGRWTA*CTHPRLCCTPSARHSFSASRRFLGRSLATSGGRTTCLYSK 139
Query: 138 VSSKYFSEYWI*PMSTNAS 82
VSSKYFSEYWI PMST A+
Sbjct: 138 VSSKYFSEYWIWPMSTTAA 82
Score = 24.0 bits (46), Expect(2) = 3e-048
Identities = 5/7 (71%), Positives = 7/7 (100%)
Frame = +3 / +3
Query: 81 EKRWWTW 101
++RWWTW
Sbjct: 81 KRRWWTW 101
>gb|BQ760103.1|BQ760103 EBpi07_SQ003_P02_R pistil, 12 DPA, no treatment, cv Optic, EBpi07
Hordeum vulgare subsp. vulgare cDNA clone
EBpi07_SQ003_P02 5', mRNA sequence
Length = 349
Score = 188 bits (404), Expect(2) = 3e-048
Identities = 71/77 (92%), Positives = 77/77 (100%)
Frame = +2 / +2
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A+VDMGQIQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQQSRGWVHYA
Sbjct: 104 AVVDMGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYA 283
Query: 266 IHRPEPHIMLFRRPINY 316
+HRPEPHIMLFRRP+NY
Sbjct: 284 VHRPEPHIMLFRRPLNY 334
Score = 173 bits (372), Expect = 1e-042
Identities = 70/79 (88%), Positives = 71/79 (89%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRRNSMMCGSGRW A*CTHPRLCCTP ARHSFS RRFLGRSLATSGGRTTCLYS
Sbjct: 336 W*LSGRRNSMMCGSGRWTA*CTHPRLCCTPSARHSFSASRRFLGRSLATSGGRTTCLYSK 157
Query: 138 VSSKYFSEYWI*PMSTNAS 82
VSSKYFSEYWI PMST A+
Sbjct: 156 VSSKYFSEYWIWPMSTTAA 100
Score = 24.0 bits (46), Expect(2) = 3e-048
Identities = 5/7 (71%), Positives = 7/7 (100%)
Frame = +3 / +3
Query: 81 EKRWWTW 101
++RWWTW
Sbjct: 99 KRRWWTW 119
>gb|CA017843.1|CA017843 HV06H11r HV Hordeum vulgare subsp. vulgare cDNA clone HV06H11
5-PRIME, mRNA sequence
Length = 500
Score = 188 bits (404), Expect = 4e-047
Identities = 71/77 (92%), Positives = 77/77 (100%)
Frame = +2 / +3
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A+VDMGQIQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQQSRGWVHYA
Sbjct: 102 AVVDMGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYA 281
Query: 266 IHRPEPHIMLFRRPINY 316
+HRPEPHIMLFRRP+NY
Sbjct: 282 VHRPEPHIMLFRRPLNY 332
Score = 173 bits (372), Expect = 1e-042
Identities = 70/79 (88%), Positives = 71/79 (89%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRRNSMMCGSGRW A*CTHPRLCCTP ARHSFS RRFLGRSLATSGGRTTCLYS
Sbjct: 334 W*LSGRRNSMMCGSGRWTA*CTHPRLCCTPSARHSFSASRRFLGRSLATSGGRTTCLYSK 155
Query: 138 VSSKYFSEYWI*PMSTNAS 82
VSSKYFSEYWI PMST A+
Sbjct: 154 VSSKYFSEYWIWPMSTTAA 98
>gb|CV060542.1|CV060542 BNEL59c5 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL59c5 5' similar to putative
cyclin-dependent kinase regulatory subunit, mRNA
sequence
Length = 375
Score = 187 bits (402), Expect = 8e-047
Identities = 71/77 (92%), Positives = 76/77 (98%)
Frame = +2 / +1
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A VDMGQIQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQQSRGWVHYA
Sbjct: 130 AAVDMGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYA 309
Query: 266 IHRPEPHIMLFRRPINY 316
+HRPEPHIMLFRRP+NY
Sbjct: 310 VHRPEPHIMLFRRPLNY 360
Score = 172 bits (370), Expect = 2e-042
Identities = 70/79 (88%), Positives = 71/79 (89%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRRNSMMCGSGRW A*CTHPRLCCTP ARHSFS RRFLGRSLATSGGRTTCLYS
Sbjct: 362 W*LSGRRNSMMCGSGRWTA*CTHPRLCCTPSARHSFSASRRFLGRSLATSGGRTTCLYSK 183
Query: 138 VSSKYFSEYWI*PMSTNAS 82
VSSKYFSEYWI PMST A+
Sbjct: 182 VSSKYFSEYWIWPMSTAAA 126
>gb|CV053867.1|CV053867 BNEL104a7 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL104a7 5' similar to putative
cyclin-dependent kinase regulatory subunit, mRNA
sequence
Length = 375
Score = 185 bits (398), Expect = 3e-046
Identities = 70/77 (90%), Positives = 75/77 (97%)
Frame = +2 / +1
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A DMGQIQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQQSRGWVHYA
Sbjct: 130 AAADMGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYA 309
Query: 266 IHRPEPHIMLFRRPINY 316
+HRPEPHIMLFRRP+NY
Sbjct: 310 VHRPEPHIMLFRRPLNY 360
Score = 170 bits (365), Expect = 1e-041
Identities = 69/79 (87%), Positives = 70/79 (88%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRRNSMMCGSGRW A*CTHPRLCCTP ARHSFS RRFLGRSLATSGGRTTCLYS
Sbjct: 362 W*LSGRRNSMMCGSGRWTA*CTHPRLCCTPSARHSFSASRRFLGRSLATSGGRTTCLYSK 183
Query: 138 VSSKYFSEYWI*PMSTNAS 82
VSSKYFSEYWI PMS A+
Sbjct: 182 VSSKYFSEYWIWPMSAAAA 126
>gb|BM377309.2|BM377309 EBem05_SQ004_L24_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ004_L24 5', mRNA sequence
Length = 355
Score = 172 bits (371), Expect(2) = 1e-043
Identities = 66/77 (85%), Positives = 72/77 (93%)
Frame = +2 / +2
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A+VDMG IQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQ RGWVHYA
Sbjct: 110 AVVDMGHIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQHIRGWVHYA 289
Query: 266 IHRPEPHIMLFRRPINY 316
HRPEPH+ML RRP+NY
Sbjct: 290 GHRPEPHMMLVRRPLNY 340
Score = 166 bits (358), Expect = 1e-040
Identities = 67/79 (84%), Positives = 70/79 (88%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRR S+MCGSGRW A*CTHPR+CCTP ARHSFS RRFLGRSLATSGGRTTCLYS
Sbjct: 342 W*LSGRRTSIMCGSGRWPA*CTHPRICCTPSARHSFSASRRFLGRSLATSGGRTTCLYSK 163
Query: 138 VSSKYFSEYWI*PMSTNAS 82
VSSKYFSEYWI PMST A+
Sbjct: 162 VSSKYFSEYWIWPMSTTAA 106
Score = 24.0 bits (46), Expect(2) = 1e-043
Identities = 5/7 (71%), Positives = 7/7 (100%)
Frame = +3 / +3
Query: 81 EKRWWTW 101
++RWWTW
Sbjct: 105 KRRWWTW 125
>gb|CV054722.1|CV054722 BNEL112e9 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL112e9 5' similar to putative
cyclin-dependent kinase regulatory subunit, mRNA
sequence
Length = 376
Score = 151 bits (324), Expect(2) = 1e-041
Identities = 58/63 (92%), Positives = 62/63 (98%)
Frame = +2 / +1
Query: 86 ALVDMGQIQYSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYA 265
A VDMGQIQYSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+ENEWRA+GVQQSRGWVHYA
Sbjct: 130 AAVDMGQIQYSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENEWRALGVQQSRGWVHYA 309
Query: 266 IHR 274
+HR
Sbjct: 310 VHR 318
Score = 138 bits (295), Expect(2) = 3e-037
Identities = 56/64 (87%), Positives = 57/64 (89%)
Frame = -3 / -3
Query: 273 RWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS*VSSKYFSEYWI*PMS 94
RW A*CTHPRLCCTP ARHSFS RRFLGRSLATSGGRTTCLYS VSSKYFSEYWI PMS
Sbjct: 317 RWTA*CTHPRLCCTPSARHSFSASRRFLGRSLATSGGRTTCLYSKVSSKYFSEYWIWPMS 138
Query: 93 TNAS 82
T A+
Sbjct: 137 TAAA 126
Score = 38.6 bits (78), Expect(2) = 1e-041
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = +2 / +2
Query: 275 PEPHIMLFRRPINY 316
PEPHIMLFRRP+NY
Sbjct: 320 PEPHIMLFRRPLNY 361
Score = 37.3 bits (75), Expect(2) = 3e-037
Identities = 14/15 (93%), Positives = 14/15 (93%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSG 274
W*L GRRNSMMCGSG
Sbjct: 363 W*LSGRRNSMMCGSG 319
>gb|BQ756119.1|BQ756119 EBem05_SQ001_N02_R embryo, 14 DPA, no treatment, cv Optic, EBem05
Hordeum vulgare subsp. vulgare cDNA clone
EBem05_SQ001_N02 5', mRNA sequence
Length = 219
Score = 165 bits (354), Expect = 3e-040
Identities = 61/68 (89%), Positives = 67/68 (98%)
Frame = +2 / +1
Query: 113 YSEKYFDDTYEYRHVVLPPEVAKLLPKNRLLSENEWRAIGVQQSRGWVHYAIHRPEPHIM 292
YSEKYFDDT+EYRHVVLPPEVAKLLPKNRLL+EN+WRA+GV QSRGWVHYA+HRPEPHIM
Sbjct: 1 YSEKYFDDTFEYRHVVLPPEVAKLLPKNRLLAENKWRALGVXQSRGWVHYAVHRPEPHIM 180
Query: 293 LFRRPINY 316
LFRRP+NY
Sbjct: 181 LFRRPLNY 204
Score = 145 bits (311), Expect = 3e-034
Identities = 60/68 (88%), Positives = 60/68 (88%)
Frame = -3 / -2
Query: 318 W*LIGRRNSMMCGSGRWIA*CTHPRLCCTPIARHSFSERRRFLGRSLATSGGRTTCLYS* 139
W*L GRRNSMMCGSGRW A*CTHPRL CTP ARH FS RRFLGRSLATSGGRTTCLYS
Sbjct: 206 W*LSGRRNSMMCGSGRWTA*CTHPRLXCTPSARHLFSASRRFLGRSLATSGGRTTCLYSK 27
Query: 138 VSSKYFSE 115
VSSKYFSE
Sbjct: 26 VSSKYFSE 3
>gb|BQ660776.1|BQ660776 HI05G14u HI Hordeum vulgare subsp. vulgare cDNA clone HI05G14
3-PRIME, mRNA sequence
Length = 438
Score = 90.0 bits (190), Expect(3) = 6e-022
Identities = 31/34 (91%), Positives = 34/34 (100%)
Frame = +2 / -2
Query: 215 EWRAIGVQQSRGWVHYAIHRPEPHIMLFRRPINY 316
EWRA+GVQQSRGWVHYA+HRPEPHIMLFRRP+NY
Sbjct: 437 EWRALGVQQSRGWVHYAVHRPEPHIMLFRRPLNY 336
Score = 31.3 bits (62), Expect(3) = 6e-022
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +3 / -3
Query: 501 WCVPCVRGNSCEGTAMC*AVTM 566
WC PCVR N+ A+C*+V +
Sbjct: 160 WCPPCVRRNAWGELAVC*SVLL 95
Score = 21.7 bits (41), Expect(3) = 6e-022
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = +2 / -1
Query: 410 PFHL*KGRRCSV 445
PFHL*KG R V
Sbjct: 225 PFHL*KGGRTLV 190
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 344,550,794
Number of Sequences: 312970
Number of extensions: 5364592
Number of successful extensions: 55019
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 8715473973
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)