TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2762998.2.1
(1036 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV925465.1|AV925465 AV925465 K. Sato unpublished cDNA li... 360 e-104
gb|BF258452.2|BF258452 HVSMEf0015M05f Hordeum vulgare seedl... 295 2e-092
gb|AF023472.1|AF023472 Hordeum vulgare peptide transporter ... 298 7e-090
gb|CA013018.1|CA013018 HT07C12r HT Hordeum vulgare subsp. v... 284 5e-076
gb|BQ659812.1|BQ659812 HF01M18w HF Hordeum vulgare subsp. v... 272 3e-072
gb|BJ454372.1|BJ454372 BJ454372 K. Sato unpublished cDNA li... 236 6e-070
gb|AV931970.1|AV931970 AV931970 K. Sato unpublished cDNA li... 262 3e-069
gb|BF259472.2|BF259472 HVSMEf0019D07f Hordeum vulgare seedl... 252 3e-066
gb|BQ464266.1|BQ464266 HF01M18T HF Hordeum vulgare subsp. v... 241 5e-063
gb|BM100095.2|BM100095 EBma01_SQ001_C23_R maternal, 4 DPA, ... 224 8e-058
gb|AV930611.1|AV930611 AV930611 K. Sato unpublished cDNA li... 210 8e-054
gb|BQ763635.1|BQ763635 EBro03_SQ005_B06_R root, 3 week, wat... 207 1e-052
gb|CA014496.1|CA014496 HT11I06r HT Hordeum vulgare subsp. v... 107 4e-050
gb|AV927146.1|AV927146 AV927146 K. Sato unpublished cDNA li... 197 8e-050
gb|BI957151.1|BI957151 HVSMEn0007M16f Hordeum vulgare rachi... 105 1e-049
gb|BJ468216.1|BJ468216 BJ468216 K. Sato unpublished cDNA li... 133 5e-048
gb|AJ435519.1|AJ435519 AJ435519 S00002 Hordeum vulgare subs... 187 1e-046
gb|CA008379.1|CA008379 HU10M10r HU Hordeum vulgare subsp. v... 146 5e-045
gb|BQ134693.1|BQ134693 LP30190 Lemma/palea-enriched cDNA li... 180 1e-044
gb|BF621907.2|BF621907 HVSMEa0001E03f Hordeum vulgare seedl... 166 4e-043
gb|BQ462946.1|BQ462946 HI02I08r HI Hordeum vulgare subsp. v... 96 5e-043
gb|AV834230.1|AV834230 AV834230 K. Sato unpublished cDNA li... 147 5e-042
gb|BI958595.1|BI958595 HVSMEn0015N02f Hordeum vulgare rachi... 160 3e-040
gb|CB878807.1|CB878807 HP09N22T HP Hordeum vulgare subsp. v... 157 4e-040
gb|BQ755329.1|BQ755329 EBed07_SQ002_F10_R endosperm, 28 DPA... 80 3e-039
gb|BJ466430.1|BJ466430 BJ466430 K. Sato unpublished cDNA li... 133 4e-039
gb|AL511639.1|AL511639 AL511639 Hordeum vulgare Barke devel... 90 7e-039
gb|AJ435085.1|AJ435085 AJ435085 S00002 Hordeum vulgare subs... 147 3e-038
gb|BF620292.3|BF620292 HVSMEc0019F20f Hordeum vulgare seedl... 157 1e-037
gb|AL504055.1|AL504055 AL504055 Hordeum vulgare Barke roots... 81 1e-036
gb|AV920121.1|AV920121 AV920121 K. Sato unpublished cDNA li... 90 5e-036
gb|BU999355.1|BU999355 HI14D24r HI Hordeum vulgare subsp. v... 150 9e-036
gb|AW983508.2|AW983508 HVSMEg0010N22f Hordeum vulgare pre-a... 143 1e-035
gb|AV920814.1|AV920814 AV920814 K. Sato unpublished cDNA li... 122 8e-035
gb|BF628254.2|BF628254 HVSMEb0003I17f Hordeum vulgare seedl... 93 1e-033
gb|AV914999.1|AV914999 AV914999 K. Sato unpublished cDNA li... 90 1e-033
gb|CA013297.1|CA013297 HT07P15r HT Hordeum vulgare subsp. v... 107 5e-033
gb|BJ458380.1|BJ458380 BJ458380 K. Sato unpublished cDNA li... 141 5e-033
gb|BE196355.3|BE196355 HVSMEh0092B05f Hordeum vulgare 5-45 ... 108 7e-033
gb|BQ463889.1|BQ463889 HG01L19r HG Hordeum vulgare subsp. v... 137 1e-031
gb|BJ462176.1|BJ462176 BJ462176 K. Sato unpublished cDNA li... 105 3e-031
gb|CA004065.1|CA004065 HS16H01r HS Hordeum vulgare subsp. v... 135 3e-031
gb|AV915705.1|AV915705 AV915705 K. Sato unpublished cDNA li... 134 6e-031
gb|BQ760868.1|BQ760868 EBro03_SQ003_L06_R root, 3 week, wat... 87 1e-030
gb|BQ464818.1|BQ464818 HU01K17T HU Hordeum vulgare subsp. v... 108 1e-030
gb|AL509456.1|AL509456 AL509456 Hordeum vulgare Barke devel... 121 1e-029
gb|BG301085.2|BG301085 HVSMEb0019I02f Hordeum vulgare seedl... 129 2e-029
gb|BG310242.2|BG310242 HVSMEc0016J19f Hordeum vulgare seedl... 128 5e-029
gb|BU989960.1|BU989960 HF23G11r HF Hordeum vulgare subsp. v... 78 7e-029
gb|BF267775.2|BF267775 HV_CEa0018N18f Hordeum vulgare seedl... 81 3e-028
gb|BU993945.1|BU993945 HM05A23r HM Hordeum vulgare subsp. v... 125 5e-028
gb|CA026875.1|CA026875 HZ57D15r HZ Hordeum vulgare subsp. v... 100 1e-026
gb|BI956939.1|BI956939 HVSMEn0006D18f Hordeum vulgare rachi... 118 6e-026
gb|BU999905.1|BU999905 HS16N14u HS Hordeum vulgare subsp. v... 117 1e-025
gb|BU999983.1|BU999983 HS16H01u HS Hordeum vulgare subsp. v... 117 1e-025
gb|CB879317.1|CB879317 HP11L18T HP Hordeum vulgare subsp. v... 96 1e-025
gb|BU998913.1|BU998913 HI12I20r HI Hordeum vulgare subsp. v... 51 3e-025
gb|AV933372.1|AV933372 AV933372 K. Sato unpublished cDNA li... 114 7e-025
gb|BE519768.1|BE519768 HV_CEb0021C13f Hordeum vulgare seedl... 87 1e-024
gb|BQ664125.1|BQ664125 HV01P15w HV Hordeum vulgare subsp. v... 111 5e-024
gb|AL509442.1|AL509442 AL509442 Hordeum vulgare Barke devel... 111 7e-024
gb|BF259400.3|BF259400 HVSMEf0019A04f Hordeum vulgare seedl... 103 7e-024
gb|BI776293.2|BI776293 EBem04_SQ001_H02_R embryo, 12 DPA, n... 108 7e-024
gb|AJ428921.1|AJ428921 AJ428921 Hordeum vulgare subsp. vulg... 110 2e-023
gb|BJ450133.1|BJ450133 BJ450133 K. Sato unpublished cDNA li... 88 2e-023
gb|BJ457560.1|BJ457560 BJ457560 K. Sato unpublished cDNA li... 88 2e-023
gb|BE421953.1|BE421953 HWM017cB.07r ITEC HWM Barley Leaf Li... 88 3e-023
gb|CA010903.1|CA010903 HT07P15u HT Hordeum vulgare subsp. v... 88 3e-023
gb|BU975381.1|BU975381 HB30P24r BC Hordeum vulgare subsp. v... 108 5e-023
gb|BQ754044.1|BQ754044 EBca01_SQ002_K11_R carpel, pre-anthe... 85 2e-022
gb|BE216021.1|BE216021 HV_CEb0009C09f Hordeum vulgare seedl... 105 4e-022
gb|AV935814.1|AV935814 AV935814 K. Sato unpublished cDNA li... 105 4e-022
gb|CD663346.1|CD663346 UCRHV18_05ag10_b1 Drought-stressed D... 105 4e-022
gb|BE216517.3|BE216517 HV_CEb0010L08f Hordeum vulgare seedl... 84 6e-022
gb|CB877683.1|CB877683 HP05N03T HP Hordeum vulgare subsp. v... 104 8e-022
gb|BM816663.1|BM816663 HB01B07_T3.ab1 HB Hordeum vulgare su... 65 2e-021
gb|CA003928.1|CA003928 HS16A10r HS Hordeum vulgare subsp. v... 75 3e-021
gb|BF255295.3|BF255295 HVSMEf0006H16f Hordeum vulgare seedl... 78 9e-021
gb|BF258688.2|BF258688 HVSMEf0016G17f Hordeum vulgare seedl... 78 9e-021
gb|BQ758892.1|BQ758892 EBma07_SQ003_K02_R maternal, 21 DPA,... 78 9e-021
gb|BU980018.1|BU980018 HA18D16r HA Hordeum vulgare subsp. v... 78 9e-021
>gb|AV925465.1|AV925465 AV925465 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24f11 5', mRNA sequence
Length = 649
Score = 360 bits (780), Expect(2) = e-104
Identities = 144/194 (74%), Positives = 172/194 (88%)
Frame = -2 / +3
Query: 858 SSMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSE 679
S+MF+EQGMVLD IGSF IPPASLSTFDV+SVI+WVP+YD ILVP+AR+FTG+E+GFSE
Sbjct: 66 STMFVEQGMVLDPTIGSFKIPPASLSTFDVVSVIIWVPIYDSILVPIARRFTGKERGFSE 245
Query: 678 LQRMGIGLVLSILAMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVGAAEV 499
LQRMGIGL++SILAM +AA++E+KRL IAR L+ + VP+SILWQIPQYFLVGA+EV
Sbjct: 246 LQRMGIGLIISILAMSAAAVLEIKRLAIAREAHLVDQNVPVPLSILWQIPQYFLVGASEV 425
Query: 498 FTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWIPDN 319
FT IG +EFFYDQ+PDAMRSLCSAL LIT +LGNY+S+ ILT+V+Y TT+GG PGWIPDN
Sbjct: 426 FTFIGALEFFYDQSPDAMRSLCSALQLITTALGNYLSAFILTMVAYFTTRGGRPGWIPDN 605
Query: 318 LNEGHLDRFFWLIA 277
LNEG LD FFWL+A
Sbjct: 606 LNEGRLDNFFWLLA 647
Score = 254 bits (550), Expect(2) = 9e-068
Identities = 122/193 (63%), Positives = 140/193 (72%)
Frame = +2 / -1
Query: 275 PAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKLLMAS 454
PA +QK SR PSFRLSGIHPG PP VV T V M L *FPS V+S SALH+LLMAS
Sbjct: 649 PASSQKKLSRRPSFRLSGIHPGLPPLVVK*ATIVRMNALR*FPSAVVMSCSALHRLLMAS 470
Query: 455 GA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTRAADT 634
G S*KNS+ P++VNTS APT+KY GIC RML GT F R + LA++ F S AA
Sbjct: 469 GDWS*KNSSAPMKVNTSEAPTRKY*GICHRMLSGTGTFWSTRCASLAMASLFISRTAAAD 290
Query: 635 IARMDRTNPIPIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGMLKDP 814
IARM+ P+PIRCSSEKP SLPVN LA GTRM S* GTH+MTL TSKV+ DAGG+L +P
Sbjct: 289 IARMEIIRPMPIRCSSEKPLSLPVNLLAIGTRMLS*IGTHIMTLTTSKVDSDAGGILNEP 110
Query: 815 MRLSRTMPCSMNM 853
+ S T+PCS NM
Sbjct: 109 IVGSSTIPCSTNM 71
Score = 71.6 bits (150), Expect(2) = 2e-025
Identities = 34/107 (31%), Positives = 55/107 (51%)
Frame = +1 / -3
Query: 277 CN*PKEPVEMAFIQIVRDPSRISSLSCNVRHQCQYDGAYIVSQ*DCNXXXXXXXXPHGIW 456
C P+E +E +Q+V DP S V Q + A +V + + PHG+
Sbjct: 647 CKQPEEVIEAPLVQVVGDPPGSPSPRREVGDHRQDERAEVVPERRGDELQRAAQAPHGVR 468
Query: 457 GLIVKELNLTNTSKHLSSAHQEILWYLPKNAHWNSSLLMNETFRPGN 597
L+V+EL + +HL A QE+L LP++A WN +L+++ PG+
Sbjct: 467 RLVVEELERPDEGEHLRGADQEVLRDLPQDAQWNRDVLVHQVRLPGD 327
Score = 64.3 bits (134), Expect(2) = 2e-025
Identities = 27/74 (36%), Positives = 47/74 (63%)
Frame = +1 / -3
Query: 634 HRENGQD*SNSHPL*LRKTLLPSSEFSS*WHQDAVIEWDPHDDADYIKSGERCRRNVERP 813
H +G +++HPL L K L + E W QDAV++ +P++D +++K G RC R++E
Sbjct: 290 HC*DGDYQADAHPLQL*KAPLLAGEPPGYWDQDAVVDRNPYNDTNHVKGG*RCWRDLE*A 111
Query: 814 NALVKNHALLYEHG 855
+ +K+H L ++HG
Sbjct: 110 DCWIKHHPLFHKHG 69
Score = 40.5 bits (82), Expect(2) = e-104
Identities = 14/17 (82%), Positives = 16/17 (94%)
Frame = -2 / +2
Query: 912 MFPIWATAIIFSAVYAQ 862
MFP+WAT I+FSAVYAQ
Sbjct: 11 MFPVWATTIVFSAVYAQ 61
Score = 23.1 bits (44), Expect(2) = 9e-068
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2 / -2
Query: 866 A*TALNMIAVAQMGN 910
A*TA N I VAQ GN
Sbjct: 57 A*TAENTIVVAQTGN 13
>gb|BF258452.2|BF258452 HVSMEf0015M05f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEf0015M05f, mRNA sequence
Length = 892
Score = 295 bits (638), Expect(2) = 2e-092
Identities = 125/198 (63%), Positives = 150/198 (75%)
Frame = -2 / +3
Query: 1035 ELXXXXXXAVIXSAXXXXXSFTDXXXXXXVXXXXELKXXXXMFPIWATAIIFSAVYAQNS 856
EL A I SF + V ELK MFP+WAT I+FSAVYAQ S
Sbjct: 18 ELRCLDKAATITDLDVKEDSFNNPWRVCTVTQVEELKILVRMFPVWATTIVFSAVYAQMS 197
Query: 855 SMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSEL 676
+MF+EQGMVLD IGSF IPPASLSTFDV+SVI+WVP+YD ILVP+AR+FTG+E+GFSEL
Sbjct: 198 TMFVEQGMVLDPTIGSFKIPPASLSTFDVVSVIIWVPIYDSILVPIARRFTGKERGFSEL 377
Query: 675 QRMGIGLVLSILAMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVGAAEVF 496
QRMGIGL++SILAM +AA++E+KRL IAR L+ + VP+SILWQIPQYFLVGA+ VF
Sbjct: 378 QRMGIGLIISILAMSAAAVLEIKRLAIAREAHLVDQNVPVPLSILWQIPQYFLVGASGVF 557
Query: 495 TCIGQVEFFYDQAPDAMR 442
T IG +EFFYDQ+PDAM+
Sbjct: 558 TFIGALEFFYDQSPDAMK 611
Score = 207 bits (446), Expect(2) = 4e-058
Identities = 100/168 (59%), Positives = 117/168 (69%)
Frame = +2 / -1
Query: 446 MASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTRA 625
MASG S*KNS+ P++VNT APT+KY GIC RML GT F R + LA++ F S A
Sbjct: 607 MASGDWS*KNSSAPMKVNTPEAPTRKY*GICHRMLSGTGTFWSTRCASLAMASLFISRTA 428
Query: 626 ADTIARMDRTNPIPIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGML 805
A IARM+ P+PIRCSSEKP SLPVN LA GTRM S* GTH+MTL TSKV+ DAGG+L
Sbjct: 427 AADIARMEIIRPMPIRCSSEKPLSLPVNLLAIGTRMLS*IGTHIMTLTTSKVDSDAGGIL 248
Query: 806 KDPMRLSRTMPCSMNMEEFWA*TALNMIAVAQMGNIXXXIFNSXXXXT 949
+P+ S T+PCS NM + A*TA N I VAQ GNI IF+S T
Sbjct: 247 NEPIVGSSTIPCSTNMVDI*A*TAENTIVVAQTGNILTNIFSSSTCVT 104
Score = 76.7 bits (161), Expect(3) = 5e-024
Identities = 33/100 (33%), Positives = 60/100 (60%)
Frame = +1 / -3
Query: 634 HRENGQD*SNSHPL*LRKTLLPSSEFSS*WHQDAVIEWDPHDDADYIKSGERCRRNVERP 813
H +G +++HPL L K L + E W QDAV++ +P++D +++K G RC R++E
Sbjct: 419 HC*DGDYQADAHPLQL*KAPLLAGEPPGYWDQDAVVDRNPYNDTNHVKGG*RCWRDLE*A 240
Query: 814 NALVKNHALLYEHGRVLGINSTEYDSSSPNGKHXXXXLQF 933
+ +K+H L ++HG L ++ +++ P+ +H LQF
Sbjct: 239 DCWIKHHPLFHKHGGHLSVDCRKHNRRCPDREHPYQYLQF 120
Score = 64.8 bits (135), Expect(2) = 2e-092
Identities = 23/40 (57%), Positives = 31/40 (77%)
Frame = -2 / +1
Query: 384 IILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISF 265
+I T+V+Y TT+GG PGWIP NLNEG L+ FF + G++F
Sbjct: 661 VIRTMVAYFTTKGGRPGWIPANLNEGRLENFFGWLKGLTF 780
Score = 47.8 bits (98), Expect(3) = 5e-024
Identities = 19/51 (37%), Positives = 32/51 (62%)
Frame = +1 / -3
Query: 445 HGIWGLIVKELNLTNTSKHLSSAHQEILWYLPKNAHWNSSLLMNETFRPGN 597
HG+ L+V+EL + +H A QE+L LP++A WN +L+++ PG+
Sbjct: 608 HGVRRLVVEELERPDEGEHS*GADQEVLRDLPQDAQWNRDVLVHQVRLPGD 456
Score = 38.6 bits (78), Expect(2) = 4e-058
Identities = 20/39 (51%), Positives = 23/39 (58%)
Frame = +2 / -3
Query: 266 KLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSM 382
K+ P + K SR PSFR +GI PG PP VV T V M
Sbjct: 779 KVKPFNHPKKFSRRPSFRFAGIQPGLPPLVVK*ATIVRM 663
Score = 24.4 bits (47), Expect(3) = 5e-024
Identities = 11/33 (33%), Positives = 16/33 (48%)
Frame = +1 / -2
Query: 286 PKEPVEMAFIQIVRDPSRISSLSCNVRHQCQYD 384
PK+ E +Q+ +P+ SLS V C D
Sbjct: 759 PKKVFEAPLVQVCGNPTGSPSLSRKVGDHCPND 661
>gb|AF023472.1|AF023472 Hordeum vulgare peptide transporter (ptr1) mRNA, complete cds
Length = 2260
Score = 298 bits (646), Expect(2) = 7e-090
Identities = 117/183 (63%), Positives = 149/183 (81%)
Frame = -2 / +3
Query: 807 FNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVS 628
F IP ASLS FD +SVI WVP+YDRILVP R TGR +GF++LQRMGIGLV+S+ AM++
Sbjct: 1374 FKIPSASLSIFDTLSVIAWVPVYDRILVPAVRSVTGRPRGFTQLQRMGIGLVVSMFAMLA 1553
Query: 627 AALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDA 448
A ++EL RL GL EK VP+SI WQ+PQYF++G AEVFT +GQ+EFFYDQAPDA
Sbjct: 1554 AGVLELVRLRTIAQHGLYGEKDVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDA 1733
Query: 447 MRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGIS 268
MRS+CSAL+L TV+LGNY+S++++T+V+ +TT+GG GWIPDNLN GHLD FFWL+A +S
Sbjct: 1734 MRSMCSALSLTTVALGNYLSTLLVTVVAKVTTRGGKQGWIPDNLNVGHLDYFFWLLAALS 1913
Query: 267 FVD 259
V+
Sbjct: 1914 LVN 1922
Score = 199 bits (430), Expect(2) = 4e-054
Identities = 96/181 (53%), Positives = 120/181 (66%)
Frame = +2 / -1
Query: 263 TKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKL 442
T+L A +QK SRWP+ RLSGIHP PP VV T V+ L *FPS TV+S SA H L
Sbjct: 1918 TRLSAASSQKK*SRWPTLRLSGIHPCLPPLVVTLATTVTSSVLR*FPSATVVSESAEHML 1739
Query: 443 LMASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTR 622
LMASGA S*KNS+ P +VNTSA P KYCG CQ+M +GT +FS P + +R +S+
Sbjct: 1738 LMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYSPCCAMVRRRTSSST 1559
Query: 623 AADTIARMDRTNPIPIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGM 802
A ++A M+ T P+P+R S P PV +GTRMRS*+GT MTL SK+ER+A G+
Sbjct: 1558 PAASMANMETTRPMPMRWSCVNPRGRPVTERTAGTRMRS*TGTQAMTLRVSKMEREAEGI 1379
Query: 803 L 805
L
Sbjct: 1378 L 1376
Score = 52.8 bits (109), Expect(2) = 7e-090
Identities = 21/46 (45%), Positives = 28/46 (60%)
Frame = -2 / +3
Query: 948 VXXXXELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIG 811
V ELK + PIWA+ I+F+ VY Q S+MF+ QG LD +G
Sbjct: 1230 VTQVEELKSVVRLLPIWASGIVFATVYGQMSTMFVLQGNTLDASMG 1367
Score = 32.2 bits (64), Expect(2) = 4e-054
Identities = 13/40 (32%), Positives = 24/40 (60%)
Frame = +1 / -3
Query: 811 PNALVKNHALLYEHGRVLGINSTEYDSSSPNGKHXXXXLQ 930
P+ V+ AL +EHG L ++ E+D++ P+G+ L+
Sbjct: 1367 PHGGVQGVALEHEHGAHLPVHRGEHDAACPDGQQAHHALE 1248
>gb|CA013018.1|CA013018 HT07C12r HT Hordeum vulgare subsp. vulgare cDNA clone HT07C12
5-PRIME, mRNA sequence
Length = 623
Score = 284 bits (615), Expect = 5e-076
Identities = 110/173 (63%), Positives = 142/173 (82%)
Frame = -2 / +2
Query: 777 FDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLE 598
FD +SVI WVP+YDRILVP R TGR +GF++LQRMGIGLV+S+ AM++A ++EL RL
Sbjct: 2 FDTLSVIAWVPVYDRILVPAVRSVTGRPRGFTQLQRMGIGLVVSMFAMLAAGVLELVRLR 181
Query: 597 IARSEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALAL 418
GL EK VP+SI WQ+PQYF++G AEVFT +GQ+EFFYDQAPDAMRS+CSAL+L
Sbjct: 182 TIAQHGLYGEKDVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSL 361
Query: 417 ITVSLGNYISSIILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
TV+LGNY+S++++T+V+ +TT+GG GWIPDNLN GHLD FFWL+A +S V+
Sbjct: 362 TTVALGNYLSTLLVTVVAKVTTRGGKQGWIPDNLNVGHLDYFFWLLAALSLVN 520
Score = 188 bits (404), Expect = 6e-047
Identities = 91/172 (52%), Positives = 112/172 (65%)
Frame = +2 / -3
Query: 263 TKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKL 442
T+L A +QK SRWP+ RLSGIHP PP VV T V+ L *FPS TV+S SA H L
Sbjct: 516 TRLSAASSQKK*SRWPTLRLSGIHPCLPPLVVTLATTVTSSVLR*FPSATVVSESAEHML 337
Query: 443 LMASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTR 622
LMASGA S*KNS+ P +VNTSA P KYCG CQ+M +GT +FS P + +R +S+
Sbjct: 336 LMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYSPCCAMVRRRTSSST 157
Query: 623 AADTIARMDRTNPIPIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSK 778
A ++A M+ T P+P+R S P PV +GTRMRS*+GT MTL SK
Sbjct: 156 PAASMANMETTRPMPMRWSCVNPRGRPVTERTAGTRMRS*TGTQAMTLRVSK 1
>gb|BQ659812.1|BQ659812 HF01M18w HF Hordeum vulgare subsp. vulgare cDNA clone HF01M18
3-PRIME, mRNA sequence
Length = 682
Score = 272 bits (588), Expect = 3e-072
Identities = 109/149 (73%), Positives = 129/149 (86%)
Frame = -2 / -2
Query: 714 RKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQ 535
R+ TG+E+GFSELQRMGIGL +S +AM AA+VE+KRLE ARSE L+H+K V MSILWQ
Sbjct: 681 RRLTGKERGFSELQRMGIGLFVSTIAMAVAAIVEIKRLESARSEDLVHQKVPVSMSILWQ 502
Query: 534 IPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYIT 355
PQY L+G EVFT IGQ EFFY+Q+PD+MRSLCSA AL+TVSLG+Y+SS ILTLVSY+T
Sbjct: 501 APQYLLIGVGEVFTSIGQAEFFYNQSPDSMRSLCSAFALVTVSLGSYLSSFILTLVSYLT 322
Query: 354 TQGGDPGWIPDNLNEGHLDRFFWLIAGIS 268
T+G + GWIPDNLNEGHLDRFFWLIAG+S
Sbjct: 321 TRGEEMGWIPDNLNEGHLDRFFWLIAGLS 235
Score = 195 bits (421), Expect = 3e-049
Identities = 93/150 (62%), Positives = 105/150 (70%)
Frame = +2 / +2
Query: 266 KLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKLL 445
+L PA++QK RSRWPSFRLSGIHP S P VV YDT VSM EL * PS+TV A A H+LL
Sbjct: 233 ELRPAMSQKKRSRWPSFRLSGIHPISSPRVVRYDTRVSMNELR*LPSDTVTRAKAEHRLL 412
Query: 446 MASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTRA 625
M SG *KNS P+ VNTS P +Y G CQRML+ T F R SDLA+S+RF ST A
Sbjct: 413 MESGDWL*KNSACPMDVNTSPTPISRY*GACQRMLMETGTF*WTRSSDLALSRRFISTIA 592
Query: 626 ADTIARMDRTNPIPIRCSSEKPFSLPVNFL 715
A IA +D PIP+R SSEKP SLPVN L
Sbjct: 593 ATAIAMVDTKRPIPMRWSSEKPLSLPVNLL 682
>gb|BJ454372.1|BJ454372 BJ454372 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24c20 5', mRNA sequence
Length = 561
Score = 236 bits (509), Expect(2) = 6e-070
Identities = 96/117 (82%), Positives = 110/117 (94%)
Frame = -2 / +3
Query: 858 SSMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSE 679
+S+F+EQGMVLDKR+GSF+IPPASL TFDVISV++W+P+YDRIL+P+ARKFTGREKGFSE
Sbjct: 210 NSVFVEQGMVLDKRVGSFDIPPASLLTFDVISVMIWIPIYDRILIPIARKFTGREKGFSE 389
Query: 678 LQRMGIGLVLSILAMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVGA 508
LQRMGIGLVLSI+ MVSAALVELKRLEIAR+EGL+HE AVPMSILWQIPQY GA
Sbjct: 390 LQRMGIGLVLSIMTMVSAALVELKRLEIARTEGLVHENVAVPMSILWQIPQYCFAGA 560
Score = 215 bits (465), Expect(2) = 5e-061
Identities = 93/116 (80%), Positives = 99/116 (85%)
Frame = +2 / -3
Query: 509 APTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTRAADTIARMDRTNPIPIRCSSEK 688
AP K+YCGICQRMLIGTA FS* RPS LAISKRFNSTRAADTI MDRTNP+PIRCSSEK
Sbjct: 559 APAKQYCGICQRMLIGTATFS*TRPSVLAISKRFNSTRAADTIVIMDRTNPMPIRCSSEK 380
Query: 689 PFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGMLKDPMRLSRTMPCSMNME 856
PFSLPVNFLA G +RS* G +MTLITSKV RDAGG+ KDP RLSRT+PCS N E
Sbjct: 379 PFSLPVNFLAMGMSIRS*IGIQIMTLITSKVNRDAGGISKDPTRLSRTIPCSTNTE 212
Score = 173 bits (373), Expect(2) = 2e-044
Identities = 73/116 (62%), Positives = 88/116 (75%)
Frame = +1 / -2
Query: 508 SAHQEILWYLPKNAHWNSSLLMNETFRPGNL*TLQLN*SCRYHRENGQD*SNSHPL*LRK 687
S + ILWYLPKNAH NS++LMN+T PGNL* LQLN SCRYH NG D*SN+H L*LR+
Sbjct: 560 STSKAILWYLPKNAHRNSNILMNKTLSPGNL*ALQLNKSCRYHCHNG*D*SNAHSL*LRE 381
Query: 688 TLLPSSEFSS*WHQDAVIEWDPHDDADYIKSGERCRRNVERPNALVKNHALLYEHG 855
TL PSSE SS ++ VI W+P D DY++S CRRN+ER N VKNH+LL+E+G
Sbjct: 380 TLFPSSELSSYGYEYTVINWNPDHDTDYVES**GCRRNIERSNPFVKNHSLLHEYG 213
Score = 161 bits (346), Expect(2) = 2e-040
Identities = 68/111 (61%), Positives = 85/111 (76%)
Frame = -1 / +2
Query: 850 VHRAGHGS*QAHWVFQHSSCISLHF*CNQRHHVGPTL*PHPGATS*KIHWKGEGFF*ATA 671
+ AG+GS*Q W+F++SSCI ++F NQ H + L*P+ S*K+HWKG+GF *ATA
Sbjct: 218 IRGAGNGS*QTGWIFRYSSCIPINFRRNQCHDLDSNL*PYTHTHS*KVHWKGKGFL*ATA 397
Query: 670 DGNWISPVHSRDGICSSS*VEAFRDCQV*RSHS*EGCCSNEHSLANTTIFL 518
+G+WISP+H +GICSS *VEA RDCQ * S S*E CCS+EHSLANTT+ L
Sbjct: 398 NGHWISPIHYDNGICSSC*VEALRDCQD*GSCS*ECCCSDEHSLANTTVLL 550
Score = 134 bits (288), Expect = 6e-031
Identities = 68/112 (60%), Positives = 74/112 (66%)
Frame = +3 / -1
Query: 507 QRPPRNIVVFAKECSLEQQPSHE*DLQTWQSLNASTQLELQIPSREWTGLIQFPSAVAQK 686
Q N VVFAKECS EQQ SHE D Q+WQSL+ASTQ ELQIP W GLIQ P AVAQ+
Sbjct: 561 QHQQSNTVVFAKECSSEQQHSHEQDPQSWQSLSASTQQELQIPLS*WIGLIQCPFAVAQR 382
Query: 687 NPSPFQ*IF*LVAPGCGHRVGPT**R*LHQKWREMQEEC*KTQCACQEPCPA 842
NP PFQ* F*L GH++ * *L +K MQEE K Q CQEP PA
Sbjct: 381 NPFPFQ*TF*LWV*VYGHKLESRS*H*LRRKLIGMQEEYRKIQPVCQEPFPA 226
Score = 111 bits (236), Expect = 9e-024
Identities = 53/78 (67%), Positives = 60/78 (76%)
Frame = -3 / +1
Query: 752 GSHSMTASWCH*LENSLEGRRVFLSYSGWELD*SCPFSRWYLQL*LS*SV*RLPGLKVSF 573
G MT *LE+SLEG+RV LSYS W LD*S P +WYLQL LS*S *RLPGL+V F
Sbjct: 316 GFQFMTVYSYP*LESSLEGKRVSLSYSEWALD*SYPL*QWYLQLLLS*SA*RLPGLRVLF 495
Query: 572 MRRLLFQ*AFFGKYHNIS 519
MR LLF+*AFFGKYH+I+
Sbjct: 496 MRMLLFR*AFFGKYHSIA 549
Score = 49.2 bits (101), Expect(2) = 6e-070
Identities = 24/60 (40%), Positives = 28/60 (46%)
Frame = -2 / +3
Query: 1035 ELXXXXXXAVIXSAXXXXXSFTDXXXXXXVXXXXELKXXXXMFPIWATAIIFSAVYAQNS 856
EL A+I S + V ELK MFP+WAT IIFS V+AQNS
Sbjct: 36 ELEFLDKAAIISSTDAKSDFSANPWRLCTVTQVEELKILVRMFPVWATTIIFSGVFAQNS 215
Score = 39.6 bits (80), Expect(2) = 5e-061
Identities = 23/60 (38%), Positives = 25/60 (41%)
Frame = +2 / -3
Query: 857 EFWA*TALNMIAVAQMGNIXXXIFNSXXXXTXXXXXXSVKDXXXXXADXXTAXXXXXSSS 1036
EF A T LNMI VAQ GNI IF+S T + D A SSS
Sbjct: 214 EF*ANTPLNMIVVAQTGNILTRIFSSSTWVTVHSLHGFAEKSLLASVDEMIAALSRNSSS 35
Score = 26.3 bits (51), Expect(2) = 2e-044
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = +1 / -2
Query: 859 VLGINSTEYDSSSPNGKHXXXXLQF 933
VL EYD S PN +H LQF
Sbjct: 212 VLSKYPAEYDCSRPNWEHSY*DLQF 138
Score = 24.9 bits (48), Expect(2) = 2e-040
Identities = 18/52 (34%), Positives = 21/52 (40%)
Frame = -3 / +1
Query: 1013 LLXSXLLXXXXXPLPTXXXXXXXXXXXN*RXXXXCFPFGLLLSYSVLFMPKT 858
LL S LL L T N*R C FG L SYS ++ +T
Sbjct: 58 LLSSHLLMPRVTFLQTHGGYALSPKWKN*RS**ECSQFGRLQSYSAGYLLRT 213
>gb|AV931970.1|AV931970 AV931970 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26p14 3', mRNA sequence
Length = 680
Score = 262 bits (566), Expect = 3e-069
Identities = 108/159 (67%), Positives = 132/159 (83%)
Frame = -2 / -1
Query: 690 GFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVG 511
GFSELQRMGIGL++SILAM +AA++E+KRL IAR L+ + VP+SILWQIPQYFLVG
Sbjct: 680 GFSELQRMGIGLIISILAMSAAAVLEIKRLAIAREAHLVDQNVPVPLSILWQIPQYFLVG 501
Query: 510 AAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGW 331
A+EVFT IG +EFFYDQ+PDAMRSLCSAL LIT +LGNY+S+ ILT+V+Y TT+GG PGW
Sbjct: 500 ASEVFTFIGALEFFYDQSPDAMRSLCSALQLITTALGNYLSAFILTMVAYFTTRGGRPGW 321
Query: 330 IPDNLNEGHLDRFFWLIAGISFVDPPPPMGCAVLLQIKK 214
IPDNLNEG LD FFWL+AG+SF++ + CA + KK
Sbjct: 320 IPDNLNEGRLDNFFWLLAGLSFLNFLVYVVCANRFKSKK 204
Score = 175 bits (377), Expect = 3e-043
Identities = 90/154 (58%), Positives = 102/154 (66%)
Frame = +2 / +1
Query: 227 NKTAHPMGGGGSTKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPS 406
N AH KL PA +QK SR PSFRLSGIHPG PP VV T V M L *FPS
Sbjct: 217 NLLAHTTYTKKFRKLSPASSQKKLSRRPSFRLSGIHPGLPPLVVK*ATIVRMNALR*FPS 396
Query: 407 ETVISASALHKLLMASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPS 586
V+S SALH+LLMASG S*KNS+ P++VNTS APT+KY GIC RML GT F R +
Sbjct: 397 AVVMSCSALHRLLMASGDWS*KNSSAPMKVNTSEAPTRKY*GICHRMLSGTGTFWSTRCA 576
Query: 587 DLAISKRFNSTRAADTIARMDRTNPIPIRCSSEK 688
LA++ F S AA IARM+ P+PIRCSSEK
Sbjct: 577 SLAMASLFISRTAAADIARMEIIRPMPIRCSSEK 678
>gb|BF259472.2|BF259472 HVSMEf0019D07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0019D07f, mRNA sequence
Length = 757
Score = 252 bits (544), Expect = 3e-066
Identities = 101/183 (55%), Positives = 134/183 (73%)
Frame = -2 / +3
Query: 807 FNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVS 628
F IP LS FD +SVI VP+YDRILVP R TGR +GF++LQRMGIGLV+S+ M++
Sbjct: 75 FKIPSDYLSIFDTLSVIGCVPVYDRILVPAVRSVTGRPRGFTQLQRMGIGLVVSMFDMLA 254
Query: 627 AALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDA 448
A ++EL RL GL EK VP+SI WQ+PQYF++ EVFT GQ+E+ YDQAPDA
Sbjct: 255 AGVLELGRLRTIAQHGLYGEKDVVPISIFWQVPQYFIISCTEVFTFSGQLEYLYDQAPDA 434
Query: 447 MRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGIS 268
MRS+C L+L TV+LGNY+ ++++T V+ +TT+GG GWIP +LN GH D FFWL+ ++
Sbjct: 435 MRSMCCELSLTTVALGNYLRTLLVTEVAKVTTRGGKQGWIPGDLNVGHFDYFFWLVDELN 614
Query: 267 FVD 259
V+
Sbjct: 615 LVN 623
Score = 165 bits (354), Expect = 5e-040
Identities = 84/181 (46%), Positives = 113/181 (62%)
Frame = +2 / -1
Query: 263 TKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKL 442
T+L + +QK S+ P+ R GIHP PP VV T+V+ L *FPS TV+S S+ H L
Sbjct: 619 TRLSSSTSQKK*SK*PTLRSPGIHPCLPPLVVTLATSVTSSVLR*FPSATVVSESSQHML 440
Query: 443 LMASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTR 622
LMASGA S K S+ P +VNTS KYCG CQ+M +GT +FS P + +R +S+
Sbjct: 439 LMASGAWSYKYSSCPEKVNTSVQLMMKYCGTCQKMEMGTTSFSPYSPCCAMVRRRPSSST 260
Query: 623 AADTIARMDRTNPIPIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGM 802
A +++ M+ T P+P+R S P PV +GTRMRS*+GT MTL SK+ER + G+
Sbjct: 259 PAASMSNMETTRPMPMRWSCVNPRGRPVTERTAGTRMRS*TGTQPMTLRVSKMER*SEGI 80
Query: 803 L 805
L
Sbjct: 79 L 77
>gb|BQ464266.1|BQ464266 HF01M18T HF Hordeum vulgare subsp. vulgare cDNA clone HF01M18
5-PRIME, mRNA sequence
Length = 663
Score = 241 bits (521), Expect = 5e-063
Identities = 97/151 (64%), Positives = 120/151 (79%)
Frame = -2 / +1
Query: 948 VXXXXELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDV 769
V ELK MFPIWAT I+F V AQNSSMFIEQGM L+ ++GSF IPPA+LS+ DV
Sbjct: 211 VTQIEELKILLRMFPIWATGIVFFTVCAQNSSMFIEQGMALNNQVGSFKIPPATLSSLDV 390
Query: 768 ISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIAR 589
IS+++WVP+Y+R +VP+AR+ TG+E+GFSELQRMGIGL +S +AM AA+VE+KRLE AR
Sbjct: 391 ISIVVWVPIYERFIVPIARRLTGKERGFSELQRMGIGLFVSTIAMAVAAIVEIKRLESAR 570
Query: 588 SEGLIHEKAAVPMSILWQIPQYFLVGAAEVF 496
SE L+H+K V MSILWQ PQY L+G EVF
Sbjct: 571 SEDLVHQKVPVSMSILWQAPQYLLIGVGEVF 663
Score = 164 bits (353), Expect = 7e-040
Identities = 81/151 (53%), Positives = 95/151 (62%)
Frame = +2 / -2
Query: 497 NTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTRAADTIARMDRTNPIPIRC 676
NTS P +Y G CQRML+ T F R SDLA+S+RF ST AA IA +D PIP+R
Sbjct: 662 NTSPTPISRY*GACQRMLMETGTF*WTRSSDLALSRRFISTIAATAIAMVDTKRPIPMRW 483
Query: 677 SSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGMLKDPMRLSRTMPCSMNME 856
SSEKP SLPVN LA GT S* GT LITS +R AGG+L DP L +PCSMN++
Sbjct: 482 SSEKPLSLPVNLLAIGTMNLS*IGTQTTMLITSNDDRVAGGILNDPTWLLSAIPCSMNID 303
Query: 857 EFWA*TALNMIAVAQMGNIXXXIFNSXXXXT 949
EF A T N + VAQ+GNI I +S T
Sbjct: 302 EF*AQTVKNTMPVAQIGNILNNILSSSICVT 210
>gb|BM100095.2|BM100095 EBma01_SQ001_C23_R maternal, 4 DPA, no treatment, cv Optic, EBma01
Hordeum vulgare subsp. vulgare cDNA clone
EBma01_SQ001_C23 5', mRNA sequence
Length = 412
Score = 224 bits (483), Expect = 8e-058
Identities = 87/136 (63%), Positives = 112/136 (82%)
Frame = -2 / +2
Query: 690 GFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVG 511
GF++LQRMGIGLV+S+ AM++A ++EL RL GL EK VP+SI WQ+PQYF++G
Sbjct: 2 GFTQLQRMGIGLVVSMFAMLAAGVLELVRLRTIAQHGLYGEKDVVPISIFWQVPQYFIIG 181
Query: 510 AAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGW 331
AEVFT +GQ+EFFYDQAPDAMRS+CSAL+L TV+LGNY+S++++T+V+ +TT GG GW
Sbjct: 182 CAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLSTLLVTVVAKVTTTGGKQGW 361
Query: 330 IPDNLNEGHLDRFFWL 283
IPDNLN HLD FFWL
Sbjct: 362 IPDNLNVRHLDYFFWL 409
Score = 149 bits (321), Expect = 2e-035
Identities = 71/136 (52%), Positives = 89/136 (65%)
Frame = +2 / -2
Query: 284 NQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKLLMASGA* 463
+QK SRW + RLSGIHP PP VV T V+ L *FPS TV+S SA H LLMASGA
Sbjct: 408 SQKK*SRWRTLRLSGIHPCLPPVVVTLATTVTSSVLR*FPSATVVSESAEHMLLMASGAW 229
Query: 464 S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTRAADTIAR 643
S*KNS+ P +VNTSA P KYCG CQ+M +GT +FS P + +R +S+ A ++A
Sbjct: 228 S*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYSPCCAMVRRRTSSSTPAASMAN 49
Query: 644 MDRTNPIPIRCSSEKP 691
M+ T P+P+R S P
Sbjct: 48 METTRPMPMRWSCVNP 1
>gb|AV930611.1|AV930611 AV930611 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd24f11 3', mRNA sequence
Length = 623
Score = 210 bits (454), Expect = 8e-054
Identities = 86/130 (66%), Positives = 104/130 (80%)
Frame = -2 / -1
Query: 603 LEIARSEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSAL 424
L IAR L+ + VP+SILWQIPQYFLVGA+EVFT IG +EFFYDQ+PDAMRSLCSAL
Sbjct: 623 LAIAREAHLVDQNVPVPLSILWQIPQYFLVGASEVFTFIGALEFFYDQSPDAMRSLCSAL 444
Query: 423 ALITVSLGNYISSIILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVDPPPPM 244
LIT +LGNY+S+ ILT+V+Y TT+GG PGWIPDNLNEG LD FFWL+AG+SF++ +
Sbjct: 443 QLITTALGNYLSAFILTMVAYFTTRGGRPGWIPDNLNEGRLDNFFWLLAGLSFLNFLVYV 264
Query: 243 GCAVLLQIKK 214
CA + KK
Sbjct: 263 VCANRFKSKK 234
Score = 141 bits (302), Expect = 7e-033
Identities = 73/125 (58%), Positives = 83/125 (66%)
Frame = +2 / +1
Query: 227 NKTAHPMGGGGSTKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPS 406
N AH KL PA +QK SR PSFRLSGIHPG PP VV T V M L *FPS
Sbjct: 247 NLLAHTTYTKKFRKLSPASSQKKLSRRPSFRLSGIHPGLPPLVVK*ATIVRMNALR*FPS 426
Query: 407 ETVISASALHKLLMASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPS 586
V+S SALH+LLMASG S*KNS+ P++VNTS APT+KY GIC RML GT F R +
Sbjct: 427 AVVMSCSALHRLLMASGDWS*KNSSAPMKVNTSEAPTRKY*GICHRMLSGTGTFWSTRCA 606
Query: 587 DLAIS 601
LA++
Sbjct: 607 SLAMA 621
>gb|BQ763635.1|BQ763635 EBro03_SQ005_B06_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ005_B06 5', mRNA sequence
Length = 704
Score = 207 bits (446), Expect = 1e-052
Identities = 79/127 (62%), Positives = 104/127 (81%)
Frame = -2 / +2
Query: 639 AMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQ 460
AM++A ++EL RL GL EK VP+SI WQ+PQYF++G AEVFT +GQ+EFFYDQ
Sbjct: 5 AMLAAGVLELVRLRTIAQHGLYGEKDVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQ 184
Query: 459 APDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLI 280
APDAMRS+CSAL+L TV+LGNY+S++++T+V+ +TT+GG GWIPDNLN GHLD FFWL+
Sbjct: 185 APDAMRSMCSALSLTTVALGNYLSTLLVTVVAKVTTRGGKQGWIPDNLNVGHLDYFFWLL 364
Query: 279 AGISFVD 259
A +S V+
Sbjct: 365 AALSLVN 385
Score = 141 bits (302), Expect = 7e-033
Identities = 68/126 (53%), Positives = 84/126 (66%)
Frame = +2 / -3
Query: 263 TKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKL 442
T+L A +QK SRWP+ RLSGIHP PP VV T V+ L *FPS TV+S SA H L
Sbjct: 381 TRLSAASSQKK*SRWPTLRLSGIHPCLPPLVVTLATTVTSSVLR*FPSATVVSESAEHML 202
Query: 443 LMASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGTAAFS*MRPSDLAISKRFNSTR 622
LMASGA S*KNS+ P +VNTSA P KYCG CQ+M +GT +FS P + +R +S+
Sbjct: 201 LMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGTTSFSPYSPCCAMVRRRTSSST 22
Query: 623 AADTIA 640
A ++A
Sbjct: 21 PAASMA 4
>gb|CA014496.1|CA014496 HT11I06r HT Hordeum vulgare subsp. vulgare cDNA clone HT11I06
5-PRIME, mRNA sequence
Length = 672
Score = 107 bits (228), Expect(3) = 4e-050
Identities = 46/79 (58%), Positives = 60/79 (75%)
Frame = -2 / +3
Query: 813 GSFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAM 634
G+ IP SL+ F V S+++ VP+YDRI+VP+AR+F G G + LQR+GIGLVLSILAM
Sbjct: 3 GTRQIPAGSLTVFFVGSILLTVPIYDRIVVPVARRFNGNPHGLTPLQRIGIGLVLSILAM 182
Query: 633 VSAALVELKRLEIARSEGL 577
SAALVE+KRL +AR +
Sbjct: 183 TSAALVEIKRLRVARESAV 239
Score = 90.4 bits (191), Expect(3) = 4e-050
Identities = 36/77 (46%), Positives = 53/77 (68%)
Frame = -2 / +3
Query: 585 EGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVS 406
E + E A VPM++ W IPQ+F VGA E FT IGQ++FF + P M+++ + L L T+S
Sbjct: 228 ESAVPEGAPVPMTVFWLIPQFFFVGAGEAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLS 407
Query: 405 LGNYISSIILTLVSYIT 355
LG ++SS ++T+V IT
Sbjct: 408 LGFFVSSALVTVVHKIT 458
Score = 39.6 bits (80), Expect(3) = 4e-050
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2 / +3
Query: 333 WIPDNLNEGHLDRFFWLIAGI 271
WI D+LN+G L +F+WL+AG+
Sbjct: 474 WIADDLNKGELYKFYWLLAGV 536
>gb|AV927146.1|AV927146 AV927146 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp. vulgare
cDNA clone basd26p14 5', mRNA sequence
Length = 635
Score = 197 bits (425), Expect = 8e-050
Identities = 85/137 (62%), Positives = 100/137 (72%)
Frame = -2 / +1
Query: 1035 ELXXXXXXAVIXSAXXXXXSFTDXXXXXXVXXXXELKXXXXMFPIWATAIIFSAVYAQNS 856
EL A I SF + V ELK MFP+WAT I+FSAVYAQ S
Sbjct: 223 ELRCLDKAATITDLDVKEDSFNNPWRVCTVTQVEELKILVRMFPVWATTIVFSAVYAQMS 402
Query: 855 SMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSEL 676
+MF+EQGMVLD IGSF IPPASLSTFDV+SVI+WVP+YD ILVP+AR+FTG+E+GFSEL
Sbjct: 403 TMFVEQGMVLDPTIGSFKIPPASLSTFDVVSVIIWVPIYDSILVPIARRFTGKERGFSEL 582
Query: 675 QRMGIGLVLSILAMVSA 625
QRMGIGL++SILAM +A
Sbjct: 583 QRMGIGLIISILAMSAA 633
Score = 145 bits (312), Expect = 3e-034
Identities = 68/109 (62%), Positives = 78/109 (71%)
Frame = +2 / -1
Query: 623 AADTIARMDRTNPIPIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLITSKVERDAGGM 802
AA IARM+ P+PIRCSSEKP SLPVN LA GTRM S* GTH+MTL TSKV+ DAGG+
Sbjct: 635 AAADIARMEIIRPMPIRCSSEKPLSLPVNLLAIGTRMLS*IGTHIMTLTTSKVDSDAGGI 456
Query: 803 LKDPMRLSRTMPCSMNMEEFWA*TALNMIAVAQMGNIXXXIFNSXXXXT 949
L +P+ S T+PCS NM + A*TA N I VAQ GNI IF+S T
Sbjct: 455 LNEPIVGSSTIPCSTNMVDI*A*TAENTIVVAQTGNILTNIFSSSTCVT 309
>gb|BI957151.1|BI957151 HVSMEn0007M16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0007M16f, mRNA sequence
Length = 1173
Score = 105 bits (225), Expect(3) = 1e-049
Identities = 46/74 (62%), Positives = 58/74 (78%)
Frame = -2 / +1
Query: 810 SFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMV 631
SF IP SL+ F V S+++ VP+Y RI+VP+AR+F G G + LQR+GIGLVLSILAM
Sbjct: 184 SFQIPAGSLTVFFVCSMLLTVPIYYRIVVPVARRFNGNPHGLTPLQRIGIGLVLSILAMT 363
Query: 630 SAALVELKRLEIAR 589
SAALVE+KRL +AR
Sbjct: 364 SAALVEIKRLRVAR 405
Score = 82.2 bits (173), Expect(3) = 1e-049
Identities = 34/77 (44%), Positives = 50/77 (64%)
Frame = -2 / +1
Query: 585 EGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVS 406
E E A VPM++ W IPQ+F VGA + FT IGQ++FF + P M+++ + L L T+S
Sbjct: 406 ESAFPEGAPVPMTVFWLIPQFFFVGAGDAFTYIGQLDFFLHECP*GMKTMSTVLFLSTLS 585
Query: 405 LGNYISSIILTLVSYIT 355
LG + SS ++T+ IT
Sbjct: 586 LGFFSSSALVTVSHMIT 636
Score = 47.8 bits (98), Expect(3) = 1e-049
Identities = 18/41 (43%), Positives = 25/41 (60%)
Frame = -2 / +1
Query: 933 ELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIG 811
E+K M PIWAT I+F VYAQ ++ + Q +D+ IG
Sbjct: 58 EVKTVARMLPIWATTIMFWTVYAQMTTFSVSQATTMDRHIG 180
>gb|BJ468216.1|BJ468216 BJ468216 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags37k17 3', mRNA sequence
Length = 699
Score = 133 bits (286), Expect(2) = 5e-048
Identities = 48/98 (48%), Positives = 74/98 (75%)
Frame = -2 / -1
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
+SI WQ+PQ+ L+ ++VF I Q+EFFY +AP +MRSLCSA + + +SLG Y++S+I++
Sbjct: 474 LSIAWQLPQFMLIACSDVFCGIAQLEFFYSEAPMSMRSLCSAFSFLAMSLGYYLNSMIIS 295
Query: 372 LVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
++ ++ GG GW+P +LN+GHLD +FWL AGI V+
Sbjct: 294 AIAALSKSGGGKGWLPADLNDGHLDYYFWLWAGIGAVN 181
Score = 78.0 bits (164), Expect(2) = 5e-048
Identities = 29/67 (43%), Positives = 52/67 (77%)
Frame = -2 / -1
Query: 801 IPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAA 622
+PPAS+++ V+ ++++V + D I++P+AR++TG G ++LQRMG+G L++ A+ +AA
Sbjct: 699 VPPASMNSASVVFMMIFVVIQDSIVIPIARRYTGNVAGLTQLQRMGVGRFLAVPALAAAA 520
Query: 621 LVELKRL 601
LVE+ RL
Sbjct: 519 LVEMWRL 499
>gb|AJ435519.1|AJ435519 AJ435519 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200061H10F1, mRNA sequence
Length = 555
Score = 187 bits (402), Expect = 1e-046
Identities = 68/102 (66%), Positives = 90/102 (88%)
Frame = -2 / +2
Query: 564 AAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISS 385
+ VP+SI WQ+PQYF++G AEVFT +GQ+EFFYDQAPDAMRS+CSAL+L TV+LGNY+S+
Sbjct: 2 SVVPISIFWQVPQYFIIGCAEVFTFVGQLEFFYDQAPDAMRSMCSALSLTTVALGNYLST 181
Query: 384 IILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
+++T+V+ +TT+GG GWIPDNLN GHLD FFWL+A +S V+
Sbjct: 182 LLVTVVAKVTTRGGKQGWIPDNLNVGHLDYFFWLLAALSLVN 307
Score = 122 bits (262), Expect = 2e-027
Identities = 60/99 (60%), Positives = 69/99 (69%)
Frame = +2 / -1
Query: 263 TKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKL 442
T+L A +QK SR P+ RLSGIHP PP VV T V+ L *FPS TV+S SA H L
Sbjct: 303 TRLSAASSQKK*SR*PTLRLSGIHPCLPPLVVTLATTVTSSVLR*FPSATVVSESAEHML 124
Query: 443 LMASGA*S*KNST*PIQVNTSAAPTKKYCGICQRMLIGT 559
LMASGA S*KNS+ P +VNTSA P KYCG CQ+M +GT
Sbjct: 123 LMASGAWS*KNSSCPTKVNTSAQPMMKYCGTCQKMEMGT 7
>gb|CA008379.1|CA008379 HU10M10r HU Hordeum vulgare subsp. vulgare cDNA clone HU10M10
5-PRIME, mRNA sequence
Length = 519
Score = 146 bits (314), Expect(2) = 5e-045
Identities = 59/123 (47%), Positives = 81/123 (65%)
Frame = -2 / +3
Query: 948 VXXXXELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDV 769
V ELK + P+WA I F+A Y Q S+ FI QG LD RIGSF +P A L+ FD
Sbjct: 39 VTQVEELKCVLRLLPVWACGITFAAAYTQMSTTFILQGDTLDPRIGSFRVPAAVLTVFDT 218
Query: 768 ISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIAR 589
+SV++WVPLYDR +VPLAR+ TG +GF++L RMG+G V+ +AM++A +E+ R +
Sbjct: 219 LSVMLWVPLYDRAVVPLARRLTGHRRGFTQLARMGVGFVILTVAMLAAGTLEVARRRVVA 398
Query: 588 SEG 580
G
Sbjct: 399 RHG 407
Score = 55.1 bits (114), Expect(2) = 5e-045
Identities = 21/27 (77%), Positives = 24/27 (88%)
Frame = -2 / +3
Query: 558 VPMSILWQIPQYFLVGAAEVFTCIGQV 478
VPMSI WQ+PQY +VGAAEVFT IGQ+
Sbjct: 438 VPMSIFWQVPQYVVVGAAEVFTFIGQM 518
>gb|BQ134693.1|BQ134693 LP30190 Lemma/palea-enriched cDNA library from dough stage of
kernel Hordeum vulgare subsp. vulgare cDNA clone 3-190
similar to Peptide transporter, similar to 15076661,
mRNA sequence
Length = 521
Score = 180 bits (388), Expect = 1e-044
Identities = 72/96 (75%), Positives = 84/96 (87%)
Frame = -2 / +1
Query: 504 EVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWIP 325
EVFT IGQ+EFFYDQAPDAMRSLC+A AL+TVS G+Y+SS ILTLVSY+TT+GGDPGWIP
Sbjct: 1 EVFTNIGQLEFFYDQAPDAMRSLCAAFALVTVSAGSYLSSFILTLVSYVTTRGGDPGWIP 180
Query: 324 DNLNEGHLDRFFWLIAGISFVDPPPPMGCAVLLQIK 217
DNLNEGHLDRFFWLIAG+SFV+ + CA+ + K
Sbjct: 181 DNLNEGHLDRFFWLIAGVSFVNLLVYISCAMKYKYK 288
Score = 138 bits (297), Expect = 4e-032
Identities = 64/80 (80%), Positives = 68/80 (85%)
Frame = +2 / -1
Query: 263 TKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMMELI*FPSETVISASALHKL 442
TKL PAINQKNRSRWPSFRLSGIHPGSPP VV DT V+M EL * P+ETV SA+A HKL
Sbjct: 242 TKLTPAINQKNRSRWPSFRLSGIHPGSPPRVVT*DTKVNMNELK*LPAETVTSANAAHKL 63
Query: 443 LMASGA*S*KNST*PIQVNT 502
LMASGA*S*KNS+*PI V T
Sbjct: 62 LMASGA*S*KNSS*PILVKT 3
Score = 115 bits (246), Expect = 4e-025
Identities = 50/89 (56%), Positives = 62/89 (69%)
Frame = -1 / +3
Query: 502 GVYLYWSS*VLLRSGPRCHEEFM*CTCTYYSLTGKLYKLHHTDIGVVHYNSGRRSWMDP* 323
G + Y S+*VLL SGPRCHE+FM C C + L+ +L+KL H D+GV+ YNS RSWMDP
Sbjct: 3 GFH*YRSA*VLL*SGPRCHEKFMCCICARHGLSRELFKLIHIDLGVLRYNSRWRSWMDPG 182
Query: 322 QSE*RPSRPVLLVNCRDKLCRSPTPHGVC 236
E RPS PVLLV+CR +LC H +C
Sbjct: 183 *PERRPS*PVLLVDCRGQLCEFAGLHQLC 269
Score = 111 bits (237), Expect = 7e-024
Identities = 48/82 (58%), Positives = 60/82 (73%)
Frame = +1 / -3
Query: 259 IYKAYPCN*PKEPVEMAFIQIVRDPSRISSLSCNVRHQCQYDGAYIVSQ*DCNXXXXXXX 438
I+KA PCN PKEPV+MAF+Q++RDPSRIS+ SCNVRHQ QY+ A I *D +
Sbjct: 246 IHKADPCNQPKEPVKMAFVQVIRDPSRISTSSCNVRHQGQYE*A*IAPC*DRDERKCST* 67
Query: 439 XPHGIWGLIVKELNLTNTSKHL 504
HGIWGLI++EL LT S++L
Sbjct: 66 TSHGIWGLIIEELKLTYISENL 1
>gb|BF621907.2|BF621907 HVSMEa0001E03f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0001E03f, mRNA sequence
Length = 594
Score = 166 bits (358), Expect(2) = 4e-043
Identities = 68/154 (44%), Positives = 104/154 (67%)
Frame = -2 / +1
Query: 789 SLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVEL 610
SL F V +I+ P+YD I+VP AR+ TG E G + LQR+G GLVLSI+AM AA+VE+
Sbjct: 1 SLPVFPVTFIILLAPIYDHIIVPFARRVTGTEMGITHLQRIGTGLVLSIVAMAVAAVVEV 180
Query: 609 KRLEIARSEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCS 430
KR +A G+ A +P++ W QY +G+A++FT G +EFF+ +AP MRSL +
Sbjct: 181 KRKNVATDAGMTDSGAPLPITFFWIAFQYLFLGSADLFTLAGLLEFFFSEAPPKMRSLAT 360
Query: 429 ALALITVSLGNYISSIILTLVSYITTQGGDPGWI 328
+L+ +++LG Y+SS+++T+V+ T +GG W+
Sbjct: 361 SLSWASLALGYYLSSVLVTVVNSATGRGGHRAWL 462
Score = 28.6 bits (56), Expect(2) = 4e-043
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = -2 / +1
Query: 321 NLNEGHLDRFFWLIAGISFVDPPPPMGCAVLLQIK 217
+LN HL RF+WL+ +S ++ +G A+ + K
Sbjct: 472 SLNHYHLQRFYWLMCVLSALNYVFFLGLAIRYKYK 576
>gb|BQ462946.1|BQ462946 HI02I08r HI Hordeum vulgare subsp. vulgare cDNA clone HI02I08
5-PRIME, mRNA sequence
Length = 640
Score = 95.9 bits (203), Expect(3) = 5e-043
Identities = 36/76 (47%), Positives = 59/76 (77%)
Frame = -2 / +2
Query: 810 SFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMV 631
SF++P AS + F +++ +W+P+YDR+LVP RK TG+++GF+ LQR GIG+ LS +AM+
Sbjct: 68 SFHVPAASFTVFAMLAQTLWIPIYDRLLVPRLRKVTGKDEGFTLLQRQGIGIALSTVAMI 247
Query: 630 SAALVELKRLEIARSE 583
+A+VE +R +IA ++
Sbjct: 248 VSAVVEDRRRDIALNQ 295
Score = 79.0 bits (166), Expect(3) = 5e-043
Identities = 29/69 (42%), Positives = 43/69 (62%)
Frame = -2 / +2
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
MS W +PQ ++G +E F I Q+EF+Y + P+ MRS+ ALA ++LGNY+S + T
Sbjct: 338 MSSFWMVPQLMILGLSEAFNLISQIEFYYKEIPEHMRSVAGALAFCNLALGNYLSGFLTT 517
Query: 372 LVSYITTQG 346
+V T G
Sbjct: 518 IVHRTTXAG 544
Score = 38.6 bits (78), Expect(3) = 5e-043
Identities = 12/21 (57%), Positives = 17/21 (80%)
Frame = -2 / +2
Query: 333 WIPDNLNEGHLDRFFWLIAGI 271
W+ +LN+G LD F+W+IAGI
Sbjct: 551 WLAQDLNKGRLDLFYWMIAGI 613
>gb|AV834230.1|AV834230 AV834230 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags7k20, mRNA sequence
Length = 628
Score = 147 bits (316), Expect(2) = 5e-042
Identities = 54/98 (55%), Positives = 77/98 (78%)
Frame = -2 / -1
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
+SI+WQ+PQYF++ AE F I Q+EFF+ QAPD+M+S+ +A AL+T +LGNY+SS I+T
Sbjct: 478 LSIVWQLPQYFVIAGAECFVIIAQLEFFHGQAPDSMKSMLTAFALLTTALGNYLSSAIIT 299
Query: 372 LVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
LV+ +T + PGWIPD+LN+GHLD F+W + IS V+
Sbjct: 298 LVAGLTREWQSPGWIPDDLNQGHLDYFYWCLTAISSVN 185
Score = 44.1 bits (90), Expect(2) = 5e-042
Identities = 21/43 (48%), Positives = 26/43 (60%)
Frame = -2 / -1
Query: 726 VPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLE 598
VP R F ++LQR+GIG + I AM AAL+E KRLE
Sbjct: 628 VPATRTFFTSGAELTQLQRIGIGRFIMIFAMAMAALLEAKRLE 500
>gb|BI958595.1|BI958595 HVSMEn0015N02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0015N02f, mRNA sequence
Length = 643
Score = 160 bits (343), Expect(2) = 3e-040
Identities = 67/131 (51%), Positives = 88/131 (67%)
Frame = -2 / +2
Query: 948 VXXXXELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDV 769
V ELK M P+WAT + F AV Q SS F+EQG +D +GS +PPAS+STFD+
Sbjct: 194 VTQVEELKILVRMLPLWATVVFFYAVSVQVSSTFVEQGRAMDAIVGSVRVPPASMSTFDI 373
Query: 768 ISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIAR 589
I++I+ VPLYDR+ VP AR+ TGRE+G SELQR+G GL + +LAM +AAL+E RL A
Sbjct: 374 ITIIVLVPLYDRVFVPAARRLTGREEGISELQRIGAGLAMPVLAMAAAALLETARLRAAE 553
Query: 588 SEGLIHEKAAV 556
+ L +V
Sbjct: 554 AAPLAPNSTSV 586
Score = 105 bits (223), Expect(2) = 1e-023
Identities = 53/119 (44%), Positives = 66/119 (55%)
Frame = +2 / -2
Query: 593 AISKRFNSTRAADTIARMDRTNPIPIRCSSEKPFSLPVNFLASGTRMRS*SGTHMMTLIT 772
A +R S AA +A P PI CSSE P SLPV+ LA+GT+ RS* GT M +I
Sbjct: 549 AARRRAVSRSAAAAMASTGMARPAPILCSSEMPSSLPVSLLAAGTKTRS*RGTRTMMVII 370
Query: 773 SKVERDAGGMLKDPMRLSRTMPCSMNMEEFWA*TALNMIAVAQMGNIXXXIFNSXXXXT 949
SKV+ DAGG +P S +PCS N++E TA VAQ G+I I +S T
Sbjct: 369 SKVDMDAGGTRTEPTMASIALPCSTNVDETCTDTA*KKTTVAQSGSIRTRILSSSTCVT 193
Score = 25.8 bits (50), Expect(2) = 3e-040
Identities = 10/21 (47%), Positives = 12/21 (57%)
Frame = -2 / +2
Query: 549 SILWQIPQYFLVGAAEVFTCI 487
S+LW PQY VG + T I
Sbjct: 581 SVLWXAPQYPPVGVGPLLTTI 643
Score = 24.9 bits (48), Expect(2) = 1e-023
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +2 / -2
Query: 494 VNTSAAPTKKYCGICQRMLI 553
V++ PT YCG C L+
Sbjct: 636 VSSGPTPTGGYCGACHSTLV 577
>gb|CB878807.1|CB878807 HP09N22T HP Hordeum vulgare subsp. vulgare cDNA clone HP09N22
5-PRIME, mRNA sequence
Length = 656
Score = 157 bits (337), Expect(2) = 4e-040
Identities = 64/143 (44%), Positives = 99/143 (69%)
Frame = -2 / +2
Query: 771 VISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIA 592
V +I+ P+YD I+VP AR+ TG E G + LQR+G GLVLSI+AM AA+VE+KR +A
Sbjct: 89 VTFIILLAPIYDHIIVPFARRVTGTEMGITHLQRIGTGLVLSIVAMAVAAVVEVKRKNVA 268
Query: 591 RSEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALIT 412
G+ A +P++ W QY +G+A++FT G +EFF+ +AP MRSL ++L+ +
Sbjct: 269 TDAGMTDSAAPLPITFFWIAFQYLFLGSADLFTLAGLLEFFFSEAPPRMRSLATSLSWAS 448
Query: 411 VSLGNYISSIILTLVSYITTQGG 343
++LG Y+SS+++T+V+ T +GG
Sbjct: 449 LALGYYLSSVLVTVVNSATGRGG 517
Score = 28.1 bits (55), Expect(2) = 4e-040
Identities = 9/18 (50%), Positives = 14/18 (77%)
Frame = -2 / +1
Query: 321 NLNEGHLDRFFWLIAGIS 268
+LN HL+RF+WL+ +S
Sbjct: 541 SLNHYHLERFYWLMCVLS 594
>gb|BQ755329.1|BQ755329 EBed07_SQ002_F10_R endosperm, 28 DPA, no treatment, cv Optic,
EBed07 Hordeum vulgare subsp. vulgare cDNA clone
EBed07_SQ002_F10 5', mRNA sequence
Length = 632
Score = 80.3 bits (169), Expect(3) = 3e-039
Identities = 30/58 (51%), Positives = 47/58 (81%)
Frame = -2 / +3
Query: 756 MWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSE 583
+W+P+YDR+LVP RK TG+++GF+ LQR GIG+ LS +AM+ +A+VE +R +IA ++
Sbjct: 9 LWIPIYDRLLVPRLRKVTGKDEGFTLLQRQGIGIALSTVAMIVSAVVEDRRRDIALNQ 182
Score = 78.0 bits (164), Expect(3) = 3e-039
Identities = 29/69 (42%), Positives = 43/69 (62%)
Frame = -2 / +3
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
MS W +PQ ++G +E F I Q+EF+Y + P+ MRS+ ALA ++LGNY+S + T
Sbjct: 225 MSSFWMVPQLMILGLSEAFNLISQIEFYYKEIPEHMRSVAGALAFCNLALGNYLSGFLTT 404
Query: 372 LVSYITTQG 346
+V T G
Sbjct: 405 IVHRTTGAG 431
Score = 42.3 bits (86), Expect(3) = 3e-039
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = -2 / +3
Query: 354 TQGGDPGWIPDNLNEGHLDRFFWLIAGI 271
T G W+ +LN+G LD F+W+IAGI
Sbjct: 417 TTGAGQNWLAQDLNKGRLDLFYWMIAGI 500
>gb|BJ466430.1|BJ466430 BJ466430 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32b11 3', mRNA sequence
Length = 595
Score = 133 bits (286), Expect(2) = 4e-039
Identities = 48/98 (48%), Positives = 74/98 (75%)
Frame = -2 / -2
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
+SI WQ+PQ+ L+ ++VF I Q+EFFY +AP +MRSLCSA + + +SLG Y++S+I++
Sbjct: 456 LSIAWQLPQFMLIACSDVFCGIAQLEFFYSEAPMSMRSLCSAFSFLAMSLGYYLNSMIIS 277
Query: 372 LVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
++ ++ GG GW+P +LN+GHLD +FWL AGI V+
Sbjct: 276 AIAALSKSGGGKGWLPADLNDGHLDYYFWLWAGIGAVN 163
Score = 48.3 bits (99), Expect(2) = 4e-039
Identities = 19/38 (50%), Positives = 29/38 (76%)
Frame = -2 / -2
Query: 714 RKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRL 601
R++TG G ++LQRMG+G L++ A+ +AALVE+ RL
Sbjct: 594 RRYTGNVAGLTQLQRMGVGRFLAVPALAAAALVEMWRL 481
>gb|AL511639.1|AL511639 AL511639 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08L15u 3',
mRNA sequence
Length = 575
Score = 90.0 bits (190), Expect(3) = 7e-039
Identities = 36/70 (51%), Positives = 53/70 (75%)
Frame = -2 / -2
Query: 564 AAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISS 385
+A MSI+WQ P YF++GAAEVF IG +EFFYDQ+P +M+SL +ALA + ++ G+Y++S
Sbjct: 433 SATSMSIMWQAPCYFVLGAAEVFASIGMLEFFYDQSPGSMKSLGAALAQLAIAGGSYLNS 254
Query: 384 IILTLVSYIT 355
+L V+ T
Sbjct: 253 ALLGAVASAT 224
Score = 65.2 bits (136), Expect(3) = 7e-039
Identities = 28/52 (53%), Positives = 38/52 (73%)
Frame = -2 / -2
Query: 729 LVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGLI 574
LVPL+R+ TG ++GF++LQR+G GL LS AM AALVE +RL A S ++
Sbjct: 565 LVPLSRRITGEDRGFTQLQRIGAGLALSTAAMAYAALVEARRLASATSMSIM 410
Score = 44.1 bits (90), Expect(3) = 7e-039
Identities = 13/23 (56%), Positives = 20/23 (86%)
Frame = -2 / -2
Query: 336 GWIPDNLNEGHLDRFFWLIAGIS 268
GWIPD+L++GHL+ F+W +A +S
Sbjct: 223 GWIPDDLDQGHLNYFYWFMAALS 155
>gb|AJ435085.1|AJ435085 AJ435085 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200019H08F1, mRNA sequence
Length = 525
Score = 147 bits (316), Expect(2) = 3e-038
Identities = 54/98 (55%), Positives = 77/98 (78%)
Frame = -2 / +3
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
+SI+WQ+PQYF++ AE F I Q+EFF+ QAPD+M+S+ +A AL+T +LGNY+SS I+T
Sbjct: 96 LSIVWQLPQYFVIAGAECFVIIAQLEFFHGQAPDSMKSMLTAFALLTTALGNYLSSAIIT 275
Query: 372 LVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
LV+ +T + PGWIPD+LN+GHLD F+W + IS V+
Sbjct: 276 LVAGLTREWQSPGWIPDDLNQGHLDYFYWCLTAISSVN 389
Score = 31.3 bits (62), Expect(2) = 3e-038
Identities = 14/23 (60%), Positives = 16/23 (69%)
Frame = -2 / +3
Query: 666 GIGLVLSILAMVSAALVELKRLE 598
GIG + I AM AAL+E KRLE
Sbjct: 6 GIGRFIMIFAMAMAALLEAKRLE 74
>gb|BF620292.3|BF620292 HVSMEc0019F20f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0019F20f, mRNA sequence
Length = 687
Score = 157 bits (337), Expect = 1e-037
Identities = 69/194 (35%), Positives = 113/194 (58%)
Frame = -2 / +3
Query: 948 VXXXXELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDV 769
V E K M PI+ T+ + + S ++QG +D R+G+ + PA+L
Sbjct: 39 VTQVEETKIVCRMIPIFLTSALGYTPVSIILSFTVQQGNTMDTRLGAIRVSPATLFLIPT 218
Query: 768 ISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIAR 589
I ++ + +YDR+LVPL R+ TG G + LQR+G+G V ++LA +AALVE +R +A
Sbjct: 219 IFQLVILVVYDRLLVPLLRRATGYVGGVTHLQRIGVGFVATVLASAAAALVETRRKGVAE 398
Query: 588 SEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITV 409
GL+ A VPMS+ W PQ+FL+G +V + +G +EFFY +A M+++ S++ +
Sbjct: 399 GSGLVDSPAGVPMSVFWLTPQFFLLGVVDVTSFVGLLEFFYSEASRGMKAIGSSVFYCML 578
Query: 408 SLGNYISSIILTLV 367
L + S++ + LV
Sbjct: 579 GLSAWFSTLTIQLV 620
>gb|AL504055.1|AL504055 AL504055 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04A21V 5', mRNA sequence
Length = 700
Score = 80.8 bits (170), Expect(3) = 1e-036
Identities = 35/69 (50%), Positives = 48/69 (69%)
Frame = -2 / +2
Query: 807 FNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVS 628
F IP SL+ F + S+++ VP+YDR++ P+AR+ TG G S LQR+ +GL LSI M +
Sbjct: 281 FLIPAGSLTVFLIGSILLTVPIYDRLVAPVARRVTGNPHGLSPLQRVFVGLFLSIAGMAA 460
Query: 627 AALVELKRL 601
AALVE RL
Sbjct: 461 AALVERHRL 487
Score = 65.7 bits (137), Expect(3) = 1e-036
Identities = 25/64 (39%), Positives = 44/64 (68%)
Frame = -2 / +2
Query: 558 VPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSII 379
V +++ +PQ+ LVGA E FT +GQ++FF + P M+++ + L L T +LG + S++I
Sbjct: 506 VTLTVFLLMPQFLLVGAGEAFTYMGQLDFFLRECPKGMKTMSTGLFLSTCALGFFFSTLI 685
Query: 378 LTLV 367
+T+V
Sbjct: 686 VTIV 697
Score = 45.1 bits (92), Expect(3) = 1e-036
Identities = 16/41 (39%), Positives = 27/41 (65%)
Frame = -2 / +2
Query: 933 ELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIG 811
E+K M PIWAT I+F ++AQ ++ +EQ V+++ +G
Sbjct: 149 EVKQVVRMLPIWATTIMFWTIHAQMTTFAVEQASVMNRAVG 271
>gb|AV920121.1|AV920121 AV920121 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9f17 3', mRNA sequence
Length = 705
Score = 90.4 bits (191), Expect(3) = 5e-036
Identities = 36/77 (46%), Positives = 53/77 (68%)
Frame = -2 / -1
Query: 585 EGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVS 406
E + E A VPM++ W IPQ+F VGA E FT IGQ++FF + P M+++ + L L T+S
Sbjct: 570 ESAVPEGAPVPMTVFWLIPQFFFVGAGEAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLS 391
Query: 405 LGNYISSIILTLVSYIT 355
LG ++SS ++T+V IT
Sbjct: 390 LGFFVSSALVTVVHKIT 340
Score = 59.7 bits (124), Expect(3) = 5e-036
Identities = 28/49 (57%), Positives = 32/49 (65%)
Frame = -2 / -1
Query: 723 PLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGL 577
PL G LQR+GIGLVLSILAM SAALVE+KRL +AR +
Sbjct: 705 PLPAASMATRTGSPRLQRIGIGLVLSILAMTSAALVEIKRLRVARESAV 559
Score = 39.6 bits (80), Expect(3) = 5e-036
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2 / -1
Query: 333 WIPDNLNEGHLDRFFWLIAGI 271
WI D+LN+G L +F+WL+AG+
Sbjct: 324 WIADDLNKGELYKFYWLLAGV 262
>gb|BU999355.1|BU999355 HI14D24r HI Hordeum vulgare subsp. vulgare cDNA clone HI14D24
5-PRIME, mRNA sequence
Length = 545
Score = 150 bits (323), Expect = 9e-036
Identities = 60/91 (65%), Positives = 74/91 (81%)
Frame = -2 / +2
Query: 486 GQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWIPDNLNEG 307
G +EFFYDQ+PDAMRSLCSAL LIT +LGNY+S+ ILT+V+Y TT+GG PGWIPDNLNEG
Sbjct: 2 GALEFFYDQSPDAMRSLCSALQLITTALGNYLSAFILTMVAYFTTRGGRPGWIPDNLNEG 181
Query: 306 HLDRFFWLIAGISFVDPPPPMGCAVLLQIKK 214
LD FFWL+AG+SF++ + CA + KK
Sbjct: 182 RLDNFFWLLAGLSFLNFLVYVVCANRFKSKK 274
>gb|AW983508.2|AW983508 HVSMEg0010N22f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0010N22f, mRNA sequence
Length = 802
Score = 143 bits (306), Expect(2) = 1e-035
Identities = 60/140 (42%), Positives = 92/140 (65%)
Frame = -2 / +1
Query: 933 ELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIM 754
E K +FPI AT +I++ V+AQ ++F +Q LD+RIGS +P A+L + +S+++
Sbjct: 109 EAKSVLKLFPIGATCLIYAVVFAQWMTLFTKQASTLDRRIGSLEVPAAALQSLISVSIVI 288
Query: 753 WVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGLI 574
+P+YD ILVPLARK++ G + LQR+G GLV+S++ MV AALVE++RL IAR GL+
Sbjct: 289 SIPIYDWILVPLARKYSKNPCGITTLQRIGTGLVISVILMVVAALVEMRRLXIAREHGLV 468
Query: 573 HEKAAVPMSILWQIPQYFLV 514
+ A W + F++
Sbjct: 469 DKPEATIPMSFWWVVPQFIL 528
Score = 27.2 bits (53), Expect(2) = 1e-035
Identities = 8/24 (33%), Positives = 17/24 (70%)
Frame = -2 / +2
Query: 411 VSLGNYISSIILTLVSYITTQGGD 340
+ +G++ISS ++ + +T+ GGD
Sbjct: 635 LGIGSFISSFLVYAIERVTSDGGD 706
>gb|AV920814.1|AV920814 AV920814 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15k17 3', mRNA sequence
Length = 679
Score = 122 bits (262), Expect(2) = 8e-035
Identities = 51/124 (41%), Positives = 82/124 (66%)
Frame = -2 / -2
Query: 885 IFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIMWVPLYDRILVPLARKF 706
I++ V+AQ ++F +Q LD+ IGS IP A+L + +S+++ +P+YD+ILVPLARK+
Sbjct: 678 IYAVVFAQWMTLFTKQASTLDRWIGSLQIPAAALQSLISVSIVISIPIYDQILVPLARKY 499
Query: 705 TGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGLIHEKAAVPMSILWQIPQ 526
+ G + LQR+G GL +S++ MV AALVE++RL +AR GL+ + A W +
Sbjct: 498 SKNPCGITPLQRIGTGLAISVILMVVAALVEMRRLRVAREYGLVDKPEATIPMSFWWVVP 319
Query: 525 YFLV 514
F++
Sbjct: 318 QFVL 307
Score = 47.3 bits (97), Expect(2) = 8e-035
Identities = 19/40 (47%), Positives = 22/40 (55%)
Frame = -2 / -2
Query: 387 SIILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGIS 268
S IL T G W DNLN GHLD F+WL+A +S
Sbjct: 180 SSILVYAIDWVTSNGGDSWFSDNLNRGHLDYFYWLLAVLS 61
>gb|BF628254.2|BF628254 HVSMEb0003I17f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0003I17f, mRNA sequence
Length = 497
Score = 93.2 bits (197), Expect(2) = 1e-033
Identities = 35/76 (46%), Positives = 58/76 (76%)
Frame = -2 / +1
Query: 810 SFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMV 631
SF++P AS + F +++ +W+P+YDR+LVP RK TG+++G + LQR GIG+ LS +AM+
Sbjct: 61 SFHVPAASFTVFAMLAQTLWIPIYDRLLVPRLRKVTGKDEGLTLLQRQGIGIALSTVAMI 240
Query: 630 SAALVELKRLEIARSE 583
+A+VE +R +IA ++
Sbjct: 241 VSAVVEDRRRDIALNQ 288
Score = 70.7 bits (148), Expect(2) = 1e-033
Identities = 25/55 (45%), Positives = 37/55 (67%)
Frame = -2 / +1
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYIS 388
MS W +PQ ++G +E F I Q+EF+Y + P+ MRS+ ALA ++LGNY+S
Sbjct: 331 MSSFWMVPQLMILGLSEAFNLISQIEFYYKEIPEHMRSVAGALAFCNLALGNYLS 495
>gb|AV914999.1|AV914999 AV914999 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9f17 5', mRNA sequence
Length = 596
Score = 90.4 bits (191), Expect(3) = 1e-033
Identities = 36/77 (46%), Positives = 53/77 (68%)
Frame = -2 / +3
Query: 585 EGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVS 406
E + E A VPM++ W IPQ+F VGA E FT IGQ++FF + P M+++ + L L T+S
Sbjct: 144 ESAVPEGAPVPMTVFWLIPQFFFVGAGEAFTYIGQLDFFLRECPKGMKTMSTGLFLSTLS 323
Query: 405 LGNYISSIILTLVSYIT 355
LG ++SS ++T+V IT
Sbjct: 324 LGFFVSSALVTVVHKIT 374
Score = 51.5 bits (106), Expect(3) = 1e-033
Identities = 25/49 (51%), Positives = 29/49 (59%)
Frame = -2 / +3
Query: 723 PLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGL 577
PL G +GIGLVLSILAM SAALVE+KRL +AR +
Sbjct: 9 PLPAASMATRTGSPRSSGIGIGLVLSILAMTSAALVEIKRLRVARESAV 155
Score = 39.6 bits (80), Expect(3) = 1e-033
Identities = 12/21 (57%), Positives = 18/21 (85%)
Frame = -2 / +3
Query: 333 WIPDNLNEGHLDRFFWLIAGI 271
WI D+LN+G L +F+WL+AG+
Sbjct: 390 WIADDLNKGELYKFYWLLAGV 452
>gb|CA013297.1|CA013297 HT07P15r HT Hordeum vulgare subsp. vulgare cDNA clone HT07P15
5-PRIME, mRNA sequence
Length = 480
Score = 107 bits (229), Expect(2) = 5e-033
Identities = 44/91 (48%), Positives = 64/91 (70%)
Frame = -2 / +2
Query: 810 SFNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMV 631
+F +PPA+L TF +++I ++P+YDR+ VP AR+FT G + LQR+G GLVL+++AMV
Sbjct: 182 TFRVPPAALQTFISLTIITFIPVYDRLFVPAARRFTRLSSGITMLQRIGTGLVLALVAMV 361
Query: 630 SAALVELKRLEIARSEGLIHEKAAVPMSILW 538
AALVE +RL +AR GL+ + A LW
Sbjct: 362 VAALVEARRLGVAREAGLVDDPKAALPMSLW 454
Score = 53.8 bits (111), Expect(2) = 5e-033
Identities = 18/42 (42%), Positives = 31/42 (73%)
Frame = -2 / +2
Query: 933 ELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGS 808
E++ +FPIWAT II++ +++Q+S+ F +Q LD+RIG+
Sbjct: 56 EVRGLLRLFPIWATCIIYAVIFSQSSTFFTKQAATLDRRIGA 181
>gb|BJ458380.1|BJ458380 BJ458380 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak31m11 3', mRNA sequence
Length = 472
Score = 141 bits (303), Expect = 5e-033
Identities = 50/95 (52%), Positives = 77/95 (81%)
Frame = -2 / -3
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
++I+WQ P Y ++G AEVFT +G +EFFYD++P++M+S+ +ALA + +S GNY++S +L
Sbjct: 470 LNIMWQTPSYVVLGVAEVFTSVGIMEFFYDESPESMKSMGAALAQLAISAGNYLNSAVLG 291
Query: 372 LVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGIS 268
+V+ T +GG PGWIPD+L+ GHLD FFW++AG+S
Sbjct: 290 VVASATGRGGAPGWIPDDLDVGHLDYFFWMMAGLS 186
>gb|BE196355.3|BE196355 HVSMEh0092B05f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0092B05f, mRNA sequence
Length = 977
Score = 108 bits (230), Expect(2) = 7e-033
Identities = 47/87 (54%), Positives = 59/87 (67%)
Frame = -2 / +1
Query: 807 FNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVS 628
F IP ASLS FD +SVI WVP+YDR LVP R TGR +GF++LQRMGIGLV S+ M++
Sbjct: 433 FKIPSASLSIFDTLSVIAWVPVYDRXLVPAVRSVTGRPRGFTQLQRMGIGLVGSMFRMLA 612
Query: 627 AALVELKRLEIARSEGLIHEKAAVPMS 547
A ++EL RL + G +K P S
Sbjct: 613 AGVLELVRLRTSXXTGCTGKKTWCPSS 693
Score = 52.8 bits (109), Expect(2) = 7e-033
Identities = 21/46 (45%), Positives = 28/46 (60%)
Frame = -2 / +1
Query: 948 VXXXXELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIG 811
V ELK + PIWA+ I+F+ VY Q S+MF+ QG LD +G
Sbjct: 289 VTQVEELKSVVRLLPIWASGIVFATVYGQMSTMFVLQGNTLDASMG 426
>gb|BQ463889.1|BQ463889 HG01L19r HG Hordeum vulgare subsp. vulgare cDNA clone HG01L19
5-PRIME, mRNA sequence
Length = 459
Score = 137 bits (293), Expect = 1e-031
Identities = 57/140 (40%), Positives = 95/140 (67%)
Frame = -2 / +1
Query: 768 ISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIAR 589
+++++ + +YDR+ V R+ T +G S L RMGIG+++ +L M +A+L+E +RL AR
Sbjct: 40 LTMLVVIVVYDRLFVKAVRRHTKNPRGISLLTRMGIGMLVQVLTMGTASLIESRRLSYAR 219
Query: 588 SEGLIHEKAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITV 409
S G + +SI +PQ+ L+G A+ F +G++EFFYDQAP++M+SL +AL+L
Sbjct: 220 SHGTDGIGGELRLSIFVLLPQFVLMGLADAFLVVGKIEFFYDQAPESMKSLGTALSLTAY 399
Query: 408 SLGNYISSIILTLVSYITTQ 349
+GN +SS IL+LV+ +T +
Sbjct: 400 GVGNVLSSFILSLVTRVTRE 459
>gb|BJ462176.1|BJ462176 BJ462176 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24c20 3', mRNA sequence
Length = 319
Score = 105 bits (224), Expect(2) = 3e-031
Identities = 42/56 (75%), Positives = 48/56 (85%)
Frame = -2 / -2
Query: 384 IILTLVSYITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVDPPPPMGCAVLLQIK 217
IILTLVSY+TTQGGD GWIPDNLNEGHLDRFFWL+AGISFV+ +GCA+ + K
Sbjct: 237 IILTLVSYLTTQGGDAGWIPDNLNEGHLDRFFWLLAGISFVNLLVYIGCAMRYKYK 70
Score = 90.4 bits (191), Expect(2) = 1e-022
Identities = 38/50 (76%), Positives = 40/50 (80%)
Frame = +2 / +2
Query: 236 AHPMGGGGSTKLIPAINQKNRSRWPSFRLSGIHPGSPP*VVMYDTNVSMM 385
A PM TKLIPA NQKNRSRWPSF+LSGIHP SPP*VV YDT V+MM
Sbjct: 89 AQPM*TSKFTKLIPASNQKNRSRWPSFKLSGIHPASPP*VVRYDTKVNMM 238
Score = 50.1 bits (103), Expect(2) = 3e-031
Identities = 19/26 (73%), Positives = 25/26 (96%)
Frame = -2 / -1
Query: 462 QAPDAMRSLCSALALITVSLGNYISS 385
QAPDAMRSLC+ALAL+ V++G+Y+SS
Sbjct: 316 QAPDAMRSLCAALALVXVTVGSYLSS 239
Score = 36.3 bits (73), Expect(2) = 1e-022
Identities = 18/26 (69%), Positives = 20/26 (76%)
Frame = +2 / +3
Query: 386 ELI*FPSETVISASALHKLLMASGA* 463
EL * P+ T SA+A HKLLMASGA*
Sbjct: 240 ELK*LPTVTXTSANAAHKLLMASGA* 317
>gb|CA004065.1|CA004065 HS16H01r HS Hordeum vulgare subsp. vulgare cDNA clone HS16H01
5-PRIME, mRNA sequence
Length = 548
Score = 135 bits (290), Expect = 3e-031
Identities = 60/105 (57%), Positives = 68/105 (64%)
Frame = -2 / +1
Query: 1035 ELXXXXXXAVIXSAXXXXXSFTDXXXXXXVXXXXELKXXXXMFPIWATAIIFSAVYAQNS 856
EL A I SF + V ELK MFP+WAT I+FSAVYAQ S
Sbjct: 232 ELRCLDKAATITDLDVKEDSFNNPWRVCTVTQVEELKILVRMFPVWATTIVFSAVYAQMS 411
Query: 855 SMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIMWVPLYDRILVP 721
+MF+EQGMVLD GSF IPPASLSTFDV+SVI+WVP+YD ILVP
Sbjct: 412 TMFVEQGMVLDPTKGSFKIPPASLSTFDVVSVIIWVPIYDSILVP 546
>gb|AV915705.1|AV915705 AV915705 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15k17 5', mRNA sequence
Length = 603
Score = 134 bits (288), Expect = 6e-031
Identities = 56/125 (44%), Positives = 86/125 (68%)
Frame = -2 / +3
Query: 933 ELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIM 754
E K +FPI AT +I++ V+AQ ++F +Q LD+ IGS IP A+L + +S+++
Sbjct: 210 EAKSVLKLFPIGATCLIYAVVFAQWMTLFTKQASTLDRWIGSLQIPAAALQSLISVSIVI 389
Query: 753 WVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSEGLI 574
+P+YD+ILVPLARK++ G + LQR+G GL +S++ MV AALVE++RL +AR GL+
Sbjct: 390 SIPIYDQILVPLARKYSKNPCGITPLQRIGTGLAISVILMVVAALVEMRRLRVAREYGLV 569
Query: 573 HEKAA 559
+ A
Sbjct: 570 DKPEA 584
>gb|BQ760868.1|BQ760868 EBro03_SQ003_L06_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ003_L06 5', mRNA sequence
Length = 611
Score = 86.8 bits (183), Expect(3) = 1e-030
Identities = 35/75 (46%), Positives = 51/75 (68%)
Frame = -2 / -3
Query: 567 KAAVPMSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYIS 388
K VPMS+ W +PQY L GAA+VFT +G EFFYDQ PD +RS+ AL + +G++IS
Sbjct: 513 KVPVPMSLWWMVPQYVLFGAADVFTMVGLQEFFYDQVPDKLRSIGLALYISIFGIGSFIS 334
Query: 387 SIILTLVSYITTQGG 343
S +++ + T++ G
Sbjct: 333 SGLVSGIDKWTSERG 289
Score = 46.4 bits (95), Expect(3) = 1e-030
Identities = 19/41 (46%), Positives = 26/41 (63%)
Frame = -2 / -3
Query: 333 WIPDNLNEGHLDRFFWLIAGISFVDPPPPMGCAVLLQIKKK 211
W +NLN GHLD F+WLIA +S ++ + AV + KKK
Sbjct: 282 WFSNNLNRGHLDYFYWLIAALSALELLVYVFFAVTFRYKKK 160
Score = 38.6 bits (78), Expect(3) = 1e-030
Identities = 17/28 (60%), Positives = 22/28 (78%)
Frame = -2 / -3
Query: 660 GLVLSILAMVSAALVELKRLEIARSEGL 577
G+VLS+++MV AALVE +RL IA GL
Sbjct: 609 GMVLSLVSMVVAALVETRRLRIATDAGL 526
>gb|BQ464818.1|BQ464818 HU01K17T HU Hordeum vulgare subsp. vulgare cDNA clone HU01K17
5-PRIME, mRNA sequence
Length = 641
Score = 108 bits (231), Expect(2) = 1e-030
Identities = 43/90 (47%), Positives = 65/90 (72%)
Frame = -2 / +3
Query: 807 FNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVS 628
F +PPA+L TF ++++ ++P+YDR+ VPLAR++TGR G + LQR+G GL LS++A+V
Sbjct: 135 FKVPPAALQTFISVTIVAFIPVYDRLFVPLARRYTGRPTGITMLQRVGAGLSLSLVAVVL 314
Query: 627 AALVELKRLEIARSEGLIHEKAAVPMSILW 538
+ALVE++RL +AR GL+ A LW
Sbjct: 315 SALVEMRRLGVARDAGLVDTPKARLPMTLW 404
Score = 45.1 bits (92), Expect(2) = 1e-030
Identities = 15/34 (44%), Positives = 24/34 (70%)
Frame = -2 / +3
Query: 912 MFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIG 811
+ PIWA II++ +++Q S+ F +Q LD+RIG
Sbjct: 27 LLPIWACCIIYAIIFSQTSTFFTKQAATLDRRIG 128
>gb|AL509456.1|AL509456 AL509456 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY01O01u 3',
mRNA sequence
Length = 700
Score = 121 bits (258), Expect(2) = 1e-029
Identities = 46/83 (55%), Positives = 64/83 (77%)
Frame = -2 / -1
Query: 507 AEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSYITTQGGDPGWI 328
AE F I Q+EFF+ QAPD+M+S+ +A AL+T +LGNY+SS I+TLV+ +T + PGWI
Sbjct: 472 AECFVIIAQLEFFHGQAPDSMKSMLTAFALLTTALGNYLSSAIITLVAGLTREWQSPGWI 293
Query: 327 PDNLNEGHLDRFFWLIAGISFVD 259
PD+LN+GHLD F+W + IS V+
Sbjct: 292 PDDLNQGHLDYFYWCLTAISSVN 224
Score = 29.5 bits (58), Expect(2) = 1e-029
Identities = 8/13 (61%), Positives = 13/13 (100%)
Frame = -2 / -3
Query: 552 MSILWQIPQYFLV 514
+SI+WQ+PQYF++
Sbjct: 518 LSIVWQLPQYFVI 480
>gb|BG301085.2|BG301085 HVSMEb0019I02f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0019I02f, mRNA sequence
Length = 541
Score = 129 bits (277), Expect = 2e-029
Identities = 46/94 (48%), Positives = 71/94 (75%)
Frame = -2 / +1
Query: 540 WQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILTLVSY 361
WQ+PQ+ L+ ++VF I Q+EFFY +AP +MRSLCSA + + +SLG Y++S+I++ ++
Sbjct: 1 WQLPQFMLIACSDVFCGIAQLEFFYSEAPMSMRSLCSAFSFLAMSLGYYLNSMIISAIAA 180
Query: 360 ITTQGGDPGWIPDNLNEGHLDRFFWLIAGISFVD 259
++ GG GW+P +LN+GHLD +FWL AGI V+
Sbjct: 181 LSKSGGGKGWLPADLNDGHLDYYFWLWAGIGAVN 282
>gb|BG310242.2|BG310242 HVSMEc0016J19f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0016J19f, mRNA sequence
Length = 787
Score = 128 bits (274), Expect = 5e-029
Identities = 49/110 (44%), Positives = 75/110 (68%)
Frame = -2 / +2
Query: 933 ELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIGSFNIPPASLSTFDVISVIM 754
E+K + P W + ++F + AQ SS IEQGM +D R+G F +PPAS++ F+V++ ++
Sbjct: 458 EVKMLFRLCPAWVSIVVFFMITAQMSSTLIEQGMAMDNRVGPFTVPPASVAGFEVVTTLV 637
Query: 753 WVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVSAALVELKR 604
+P+YD LVPLAR+ TG+++G S LQR+G+G +S L M A L+E R
Sbjct: 638 LIPVYDAALVPLARRATGQDRGLSLLQRLGVGFAVSALGMAYAGLLERNR 787
>gb|BU989960.1|BU989960 HF23G11r HF Hordeum vulgare subsp. vulgare cDNA clone HF23G11
5-PRIME, mRNA sequence
Length = 623
Score = 78.0 bits (164), Expect(3) = 7e-029
Identities = 29/69 (42%), Positives = 43/69 (62%)
Frame = -2 / +3
Query: 552 MSILWQIPQYFLVGAAEVFTCIGQVEFFYDQAPDAMRSLCSALALITVSLGNYISSIILT 373
MS W +PQ ++G +E F I Q+EF+Y + P+ MRS+ ALA ++LGNY+S + T
Sbjct: 171 MSSFWMVPQLMILGLSEAFNLISQIEFYYKEIPEHMRSVAGALAFCNLALGNYLSGFLTT 350
Query: 372 LVSYITTQG 346
+V T G
Sbjct: 351 IVHRTTGAG 377
Score = 45.1 bits (92), Expect(3) = 7e-029
Identities = 18/40 (45%), Positives = 31/40 (77%)
Frame = -2 / +3
Query: 702 GREKGFSELQRMGIGLVLSILAMVSAALVELKRLEIARSE 583
G+++G + LQR GIG+ LS +AM+ +A+VE +R +IA ++
Sbjct: 9 GKDEGLTLLQRQGIGIALSTVAMIVSAVVEDRRRDIALNQ 128
Score = 42.3 bits (86), Expect(3) = 7e-029
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = -2 / +3
Query: 354 TQGGDPGWIPDNLNEGHLDRFFWLIAGI 271
T G W+ +LN+G LD F+W+IAGI
Sbjct: 363 TTGAGQNWLAQDLNKGRLDLFYWMIAGI 446
>gb|BF267775.2|BF267775 HV_CEa0018N18f Hordeum vulgare seedling green leaf EST library
HVcDNA0004 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEa0018N18f, mRNA sequence
Length = 817
Score = 80.8 bits (170), Expect(3) = 3e-028
Identities = 35/69 (50%), Positives = 48/69 (69%)
Frame = -2 / +2
Query: 807 FNIPPASLSTFDVISVIMWVPLYDRILVPLARKFTGREKGFSELQRMGIGLVLSILAMVS 628
F IP SL+ F + S+++ VP+YDR++ P+AR+ TG G S LQR+ +GL LSI M +
Sbjct: 437 FLIPAGSLTVFLIGSILLTVPIYDRLVAPVARRVTGNPHGLSPLQRVFVGLFLSIAGMAA 616
Query: 627 AALVELKRL 601
AALVE RL
Sbjct: 617 AALVERHRL 643
Score = 45.1 bits (92), Expect(3) = 3e-028
Identities = 16/41 (39%), Positives = 27/41 (65%)
Frame = -2 / +2
Query: 933 ELKXXXXMFPIWATAIIFSAVYAQNSSMFIEQGMVLDKRIG 811
E+K M PIWAT I+F ++AQ ++ +EQ V+++ +G
Sbjct: 305 EVKQVVRMLPIWATTIMFWTIHAQMTTFAVEQASVMNRAVG 427
Score = 37.7 bits (76), Expect(3) = 3e-028
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = -2 / +2
Query: 558 VPMSILWQIPQYFLVGAAEVFTCIGQVEFF 469
V +++ +PQ LVGA E FT +GQ++FF
Sbjct: 662 VTLTVFLLMPQXLLVGAGEAFTYMGQLDFF 751
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 500,636,704
Number of Sequences: 312970
Number of extensions: 8536317
Number of successful extensions: 42339
Number of sequences better than 1.0e-020: 108
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 12294291788
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)