TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750763.3.1
(819 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachi... 214 3e-091
gb|AW983378.3|AW983378 HVSMEg0010G22f Hordeum vulgare pre-a... 164 7e-040
gb|BI959049.1|BI959049 HVSMEn0017O16f Hordeum vulgare rachi... 126 1e-028
>gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018A17f, mRNA sequence
Length = 632
Score = 214 bits (462), Expect(2) = 3e-091
Identities = 83/92 (90%), Positives = 89/92 (96%)
Frame = +1 / +3
Query: 544 NPRKGTLRYAVIHEEPLWIIFKRDMVITLKEELIMNSFKTIDGRGANVHIANGACITIQY 723
+P+KGTLRYAVI +EPLWIIFKRDMVITL +ELIMNSFKTIDGRGANVHIANGAC TIQY
Sbjct: 168 DPKKGTLRYAVIQDEPLWIIFKRDMVITLSQELIMNSFKTIDGRGANVHIANGACTTIQY 347
Query: 724 ITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWR 819
+TNVIIHGLHIHDC+PTGNAMVRSSPSHYGWR
Sbjct: 348 VTNVIIHGLHIHDCRPTGNAMVRSSPSHYGWR 443
Score = 141 bits (302), Expect(2) = 3e-091
Identities = 51/59 (86%), Positives = 54/59 (91%)
Frame = +1 / +2
Query: 382 GTGNPIDDCWRCDSDWHNNRKRLADCGIGFGRNAIGGRDGKIYVVTDPSDDDPVNPRKG 558
G GNPIDDCWRCDSDWHNNR+RLADCGIGFGRNAIGGRDGKIYVVTD DDDP P++G
Sbjct: 5 GRGNPIDDCWRCDSDWHNNRQRLADCGIGFGRNAIGGRDGKIYVVTDAGDDDPGRPQEG 181
Score = 121 bits (258), Expect(4) = 3e-059
Identities = 45/50 (90%), Positives = 46/50 (92%)
Frame = -2 / -2
Query: 818 RHP*WLGELRTMAFPVGLQSWMWSPWMMTLVMYWMVMQAPLAMCTLAPRP 669
RHP*WLGELRTMA PVG QSWMW PWMMTLV YWMV+QAPLAMCTLAPRP
Sbjct: 442 RHP*WLGELRTMALPVGRQSWMWRPWMMTLVTYWMVVQAPLAMCTLAPRP 293
Score = 105 bits (223), Expect(2) = 8e-028
Identities = 43/55 (78%), Positives = 48/55 (87%)
Frame = -1 / -1
Query: 708 AGTIGDVHVGAASVDGLEAVHDELLLERDDHVPLEDDPERLFVDDGVAEGALARV 544
AG +GDVHVGAA+VDGLEAVHDELL E DDHV LEDDP+RL +DDGVA+GAL V
Sbjct: 332 AGAVGDVHVGAAAVDGLEAVHDELLAEGDDHVALEDDPQRLVLDDGVAQGALLGV 168
Score = 93.6 bits (198), Expect(4) = 3e-059
Identities = 39/42 (92%), Positives = 39/42 (92%)
Frame = -2 / -3
Query: 506 ILPSRPPMALRPKPMPQSARRFRLLCQSESQRQQSSMGLPVP 381
ILPSRPPMALRPKPMPQSARR RLLCQSESQRQQSSMG P P
Sbjct: 129 ILPSRPPMALRPKPMPQSARRCRLLCQSESQRQQSSMGFPRP 4
Score = 39.1 bits (79), Expect(4) = 3e-059
Identities = 16/17 (94%), Positives = 16/17 (94%)
Frame = -2 / -2
Query: 605 KMIQSGSSWMTA*RRVP 555
KMI SGSSWMTA*RRVP
Sbjct: 229 KMIHSGSSWMTA*RRVP 179
Score = 38.6 bits (78), Expect(2) = 8e-028
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = -3 / -3
Query: 817 ATHSGWASCAPWRSPW 770
AT SGW SCAPWR W
Sbjct: 441 ATRSGWGSCAPWRCRW 394
Score = 31.8 bits (63), Expect(4) = 3e-059
Identities = 12/13 (92%), Positives = 13/13 (100%)
Frame = -1 / -2
Query: 540 RVVIARVGDHVDL 502
RVV+ARVGDHVDL
Sbjct: 163 RVVVARVGDHVDL 125
>gb|AW983378.3|AW983378 HVSMEg0010G22f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0010G22f, mRNA sequence
Length = 809
Score = 164 bits (352), Expect = 7e-040
Identities = 66/114 (57%), Positives = 78/114 (68%)
Frame = +1 / +2
Query: 379 CGTGNPIDDCWRCDSDWHNNRKRLADCGIGFGRNAIGGRDGKIYVVTDPSDDDPVNPRKG 558
C NPID CWRC DW +R+RLA C GFGR A GG GKIY+VTDPSD+D NPR G
Sbjct: 272 CLATNPIDRCWRCRKDWATDRQRLARCAKGFGRGATGGLHGKIYIVTDPSDEDFTNPRPG 451
Query: 559 TLRYAVIHEEPLWIIFKRDMVITLKEELIMNSFKTIDGRGANVHIANGACITIQ 720
TLR+ V+ EPLWIIF RDM+I +E+I+ S K + GR A VHI +TIQ
Sbjct: 452 TLRWGVVQLEPLWIIFARDMIINPTQEIIIQSNKNLYGRRAQVHIGKRGGLTIQ 613
>gb|BI959049.1|BI959049 HVSMEn0017O16f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0017O16f, mRNA sequence
Length = 684
Score = 126 bits (270), Expect = 1e-028
Identities = 47/49 (95%), Positives = 49/49 (100%)
Frame = +1 / +3
Query: 673 RGANVHIANGACITIQYITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWR 819
RGANVHIANGACITIQY+TNVIIHGLHIHDC+PTGNAMVRSSPSHYGWR
Sbjct: 18 RGANVHIANGACITIQYVTNVIIHGLHIHDCRPTGNAMVRSSPSHYGWR 164
Score = 119 bits (255), Expect = 2e-026
Identities = 45/49 (91%), Positives = 45/49 (91%)
Frame = -2 / -3
Query: 818 RHP*WLGELRTMAFPVGLQSWMWSPWMMTLVMYWMVMQAPLAMCTLAPR 672
RHP*WLGELRTMA PVG QSWMW PWMMTLV YWMVMQAPLAMCTLAPR
Sbjct: 163 RHP*WLGELRTMALPVGRQSWMWRPWMMTLVTYWMVMQAPLAMCTLAPR 17
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 324,284,005
Number of Sequences: 312970
Number of extensions: 4561103
Number of successful extensions: 56039
Number of sequences better than 1.0e-020: 5
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 9262822580
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)