TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2750698.2.1
(1126 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ753852.1|BQ753852 EBca01_SQ002_A14_R carpel, pre-anthe... 386 e-107
gb|BU988919.1|BU988919 HF19C15r HF Hordeum vulgare subsp. v... 376 e-103
gb|BQ758765.1|BQ758765 EBma07_SQ002_B22_R maternal, 21 DPA,... 171 5e-042
gb|BJ467379.1|BJ467379 BJ467379 K. Sato unpublished cDNA li... 133 2e-030
>gb|BQ753852.1|BQ753852 EBca01_SQ002_A14_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ002_A14 5', mRNA sequence
Length = 631
Score = 386 bits (838), Expect = e-107
Identities = 161/179 (89%), Positives = 169/179 (94%)
Frame = +1 / +1
Query: 10 AALQSSGQNCAGAERFYVHDDIYSAFVSQIVKTVKSISVGPPLSGRYDMGAICMIEHSEK 189
AALQSSGQNCAGAERFYVH DIY FVS++VK +KSISVGPPLSGRYDMGAICMIEHSE+
Sbjct: 94 AALQSSGQNCAGAERFYVHKDIYPTFVSRVVKIIKSISVGPPLSGRYDMGAICMIEHSER 273
Query: 190 LQNLVNDALDKGAEIAVRGSFGNLGEDAVDQFFPPTGLVNVDHTMKIMQEETFGPIIPIM 369
LQNLVNDALDKGAEIA RGSFGNLGEDAVDQFFPPT LVNV+HTMKIMQEE FGPI+PIM
Sbjct: 274 LQNLVNDALDKGAEIAGRGSFGNLGEDAVDQFFPPTVLVNVNHTMKIMQEEAFGPILPIM 453
Query: 370 KFSSDEEAIKLANDSKYGLGCAVFSGNQKRAIRIASQLHCGVAAINDFASSYMCQSLPF 546
KFSSDEE I+LANDSKYGLGCAVFSGNQKRAI+IASQL CGVAAIND ASSYMCQSLPF
Sbjct: 454 KFSSDEEVIQLANDSKYGLGCAVFSGNQKRAIKIASQLQCGVAAINDXASSYMCQSLPF 630
Score = 326 bits (706), Expect = 1e-088
Identities = 147/178 (82%), Positives = 154/178 (86%)
Frame = -3 / -3
Query: 542 GKDWHM*LEAKSLIAATPQCNWDAILMARFWLPEKTAQPRPYFESFASLIASSSELNFMI 363
GKD H+*LEA SLIAATP C+ DAILMARFWLPEKTAQPRPY ESFAS + SSSE NFMI
Sbjct: 626 GKD*HI*LEAXSLIAATPHCS*DAILMARFWLPEKTAQPRPYLESFASWMTSSSEENFMI 447
Query: 362 GIIGPNVSSCIIFIV*STFTRPVGGKN*STASSPRLPKLPLTAISAPLSRASLTRF*SFS 183
G IGPN SSCIIFIV* TFTR VGGKN*STASSP+ PKLPL AISAPLSRASLTRF*S S
Sbjct: 446 GRIGPNASSCIIFIV*LTFTRTVGGKN*STASSPKFPKLPLPAISAPLSRASLTRF*SLS 267
Query: 182 ECSIIQIAPMSYLPDNGGPTLIDLTVFTICETKAE*MSS*T*NLSAPAQFCPLDCRAA 9
ECSIIQIAP+SYLPDNGGPT IDL +FT ETK *+S T*NLSAPAQFCPLDC AA
Sbjct: 266 ECSIIQIAPISYLPDNGGPTDIDLMIFTTRETKVG*ISLWT*NLSAPAQFCPLDCSAA 93
Score = 301 bits (652), Expect = 4e-081
Identities = 128/178 (71%), Positives = 141/178 (79%)
Frame = -2 / -2
Query: 540 QRLAHVT*SKIIDCCYPTV*LGRYSDGTLLVARKNSTAKTIF*VVCKFDSLFIRTEFHDW 361
QRL H+T*S IIDCC +*L RY DGTLLVARKNST KTIF ++CK D LFIR EFHDW
Sbjct: 624 QRLTHIT*SXIIDCCNTAL*LRRYFDGTLLVARKNSTTKTIFRIICKLDDLFIRREFHDW 445
Query: 360 YNRSKCLFLHNFHCVINIHQASWWEKLINCIFPKIAKTPPDGNFGTFV*SIINKILKFLR 181
NR +CLFLHNFHCVINIH SW ++LIN IF +I K P NFGTFV SIINKILK LR
Sbjct: 444 *NRPECLFLHNFHCVINIH*NSWGKELINSIFTQIPKAPSASNFGTFVKSIINKILKSLR 265
Query: 180 VLNHTDCTHVISA**WGANTYRFDCLHYL*NKGRIDVIVNIKSFSTSAILPTRLQSSS 7
VL HTDCTH++SA *WG N Y FD LHY NKGRID++V+IKSFSTS IL TRLQ SS
Sbjct: 264 VLYHTDCTHIVSAR*WGPNRY*FDDLHYARNKGRIDILVDIKSFSTSTILSTRLQCSS 91
Score = 242 bits (523), Expect = 3e-063
Identities = 123/178 (69%), Positives = 132/178 (74%)
Frame = +2 / +2
Query: 8 ELLCNLVGRIALVLKDFMFTMTSILPLFHR**RQSNL*VLAPHYQADMTWVQSV*LSTLR 187
EL CNLV +I LVLKDFM T SILPLF **R SN +L PHY AD WVQSV* STLR
Sbjct: 92 ELHCNLVDKIVLVLKDFMSTRISILPLFLA**RSSNQYLLGPHYLADTIWVQSV**STLR 271
Query: 188 NFRILLMML*TKVPKLPSGGVLAILGKMQLINFSHQLAW*MLITQ*KLCKKRHLDLLYQS 367
+FRILLMML TKVPKL + G L I KM LIN QL *MLITQ*KLC+KRH YQS
Sbjct: 272 DFRILLMMLLTKVPKLLAEGALGIWVKMLLINSFPQLF**MLITQ*KLCRKRHSGRFYQS 451
Query: 368 *NSVLMKRLSNLQTTQNMVLAVLFFRATRSVPSE*RPSYTVG*QQSMILLQVTCASLC 541
*NS+LMKR SNLQ NMVL VLFF ATRSVPS+*R +Y+ QQSMI LQV C SLC
Sbjct: 452 *NSLLMKRSSNLQMILNMVLVVLFFLATRSVPSK*RLNYSAVLQQSMIXLQVICVSLC 625
Score = 237 bits (512), Expect = 9e-062
Identities = 118/178 (66%), Positives = 128/178 (71%)
Frame = -1 / -1
Query: 541 AKTGTCNLKQNH*LLLPHSVTGTLF*WHASGCPKKQHSQDHILSRLQV**PLHQN*IS*L 362
AKT T NLK NH*LL +V TLF*WHASGC KKQH+QDHI + LQV *PLHQ IS*L
Sbjct: 625 AKTDTYNLKXNH*LLQHRTVVETLF*WHASGCQKKQHNQDHI*NHLQVG*PLHQKRIS*L 446
Query: 361 V**VQMSLLA*FSLCDQHSPGQLVGKIDQLHLPQDCQNSP*RQFRHLCLEHH*QDSEVSQ 182
V +M L A*FSLCD HS QL +IDQ HL + Q+S +QFRHLC EHH*QDSEVSQ
Sbjct: 445 VESARMPLPA*FSLCD*HSLEQLGERIDQQHLHPNSQSSLCQQFRHLCQEHH*QDSEVSQ 266
Query: 181 SAQSYRLHPCHICLIMGGQHL*I*LSSLSVKQRQNRCHREHKIFQHQRNSAH*IAEQL 8
SA SYRLHP IC IMG Q + I* SSL KQR +R HKIFQHQ N H*IA QL
Sbjct: 265 SALSYRLHPYRICQIMGAQQILI**SSLREKQR*DRYPCGHKIFQHQHNFVH*IAVQL 92
Score = 148 bits (318), Expect(2) = 4e-059
Identities = 65/99 (65%), Positives = 69/99 (69%)
Frame = +3 / +3
Query: 246 EFWQSWGRCS*SIFPTNWPGEC*SHNENYARRDIWTYYTNHEIQF**RGYQTCKRLKIWS 425
E W+ RC *SI N EC*SHNENYA R I TNHEI F**RG+ TCK IWS
Sbjct: 330 ELWEFG*RCC*SILSPNCSSEC*SHNENYAGRGIRADSTNHEILF**RGHPTCK*F*IWS 509
Query: 426 WLCCFFGQPEACHQNSVPVTLWGSSNQ*FCFKLHVPVFA 542
WLCCFF QPEACHQNSV T+ SNQ* CFKL+V VFA
Sbjct: 510 WLCCFFWQPEACHQNSVSTTVRCCSNQ*XCFKLYVSVFA 626
Score = 100 bits (213), Expect(2) = 4e-059
Identities = 43/63 (68%), Positives = 49/63 (77%)
Frame = +3 / +3
Query: 9 SCSAI*WAELRWC*KILCSR*HLFCLCFTDSEDSQIYKCWPPIIRQI*HGCNLYD*AL*E 188
SC+AI*W +L WC*KILC + +L LCF+ SED QI CW PII QI +GCNLYD AL*E
Sbjct: 93 SCTAI*WTKLCWC*KILCPQGYLSYLCFSRSEDHQINICWAPIIWQIRYGCNLYDRAL*E 272
Query: 189 TSE 197
TSE
Sbjct: 273 TSE 281
>gb|BU988919.1|BU988919 HF19C15r HF Hordeum vulgare subsp. vulgare cDNA clone HF19C15
5-PRIME, mRNA sequence
Length = 542
Score = 376 bits (816), Expect = e-103
Identities = 156/172 (90%), Positives = 164/172 (95%)
Frame = +1 / +1
Query: 70 DIYSAFVSQIVKTVKSISVGPPLSGRYDMGAICMIEHSEKLQNLVNDALDKGAEIAVRGS 249
DIY FVS++VK +KSISVGPPLSGRYDMGAICMIEHSE+LQNLVNDALDKGAEIA RGS
Sbjct: 25 DIYPTFVSRVVKIIKSISVGPPLSGRYDMGAICMIEHSERLQNLVNDALDKGAEIAGRGS 204
Query: 250 FGNLGEDAVDQFFPPTGLVNVDHTMKIMQEETFGPIIPIMKFSSDEEAIKLANDSKYGLG 429
FGNLGEDAVDQFFPPT LVNV+HTMKIMQEE FGPI+PIMKFSSDEE I+LANDSKYGLG
Sbjct: 205 FGNLGEDAVDQFFPPTVLVNVNHTMKIMQEEAFGPILPIMKFSSDEEVIQLANDSKYGLG 384
Query: 430 CAVFSGNQKRAIRIASQLHCGVAAINDFASSYMCQSLPFGGVKDSGFGRFAG 585
CAVFSGNQKRAI+IASQL CGVAAINDFASSYMCQSLPFGGVKDSGFGRFAG
Sbjct: 385 CAVFSGNQKRAIKIASQLQCGVAAINDFASSYMCQSLPFGGVKDSGFGRFAG 540
Score = 291 bits (630), Expect = 5e-078
Identities = 131/158 (82%), Positives = 138/158 (87%)
Frame = -3 / -1
Query: 542 GKDWHM*LEAKSLIAATPQCNWDAILMARFWLPEKTAQPRPYFESFASLIASSSELNFMI 363
GKD H+*LEAKSLIAATP C+ DAILMARFWLPEKTAQPRPY ESFAS + SSSE NFMI
Sbjct: 497 GKD*HI*LEAKSLIAATPHCS*DAILMARFWLPEKTAQPRPYLESFASWMTSSSEENFMI 318
Query: 362 GIIGPNVSSCIIFIV*STFTRPVGGKN*STASSPRLPKLPLTAISAPLSRASLTRF*SFS 183
G IGPN SSCIIFIV* TFTR VGGKN*STASSP+ PKLPL AISAPLSRASLTRF*S S
Sbjct: 317 GRIGPNASSCIIFIV*LTFTRTVGGKN*STASSPKFPKLPLPAISAPLSRASLTRF*SLS 138
Query: 182 ECSIIQIAPMSYLPDNGGPTLIDLTVFTICETKAE*MS 69
ECSIIQIAP+SYLPDNGGPT IDL +FT ETK *+S
Sbjct: 137 ECSIIQIAPISYLPDNGGPTDIDLMIFTTRETKVG*IS 24
Score = 284 bits (615), Expect = 5e-076
Identities = 119/172 (69%), Positives = 134/172 (77%)
Frame = -2 / -3
Query: 582 SKSPESAVFNTTEWQRLAHVT*SKIIDCCYPTV*LGRYSDGTLLVARKNSTAKTIF*VVC 403
S+ + AVFNT + QRL H+T*SKIIDCC +*L RY DGTLLVARKNST KTIF ++C
Sbjct: 537 SEPSKPAVFNTAKGQRLTHIT*SKIIDCCNTAL*LRRYFDGTLLVARKNSTTKTIFRIIC 358
Query: 402 KFDSLFIRTEFHDWYNRSKCLFLHNFHCVINIHQASWWEKLINCIFPKIAKTPPDGNFGT 223
K D LFIR EFHDW NR +CLFLHNFHCVINIH SW ++LIN IF +I K P NFGT
Sbjct: 357 KLDDLFIRREFHDW*NRPECLFLHNFHCVINIH*NSWGKELINSIFTQIPKAPSASNFGT 178
Query: 222 FV*SIINKILKFLRVLNHTDCTHVISA**WGANTYRFDCLHYL*NKGRIDVI 67
FV SIINKILK LRVL HTDCTH++SA *WG N Y FD LHY NKGRID++
Sbjct: 177 FVKSIINKILKSLRVLYHTDCTHIVSAR*WGPNRY*FDDLHYARNKGRIDIL 22
Score = 226 bits (488), Expect = 2e-058
Identities = 111/170 (65%), Positives = 124/170 (72%)
Frame = -1 / -2
Query: 583 QQISRIRCL*HHRMAKTGTCNLKQNH*LLLPHSVTGTLF*WHASGCPKKQHSQDHILSRL 404
Q+ + RCL*H + AKT T NLKQNH*LL +V TLF*WHASGC KKQH+QDHI + L
Sbjct: 538 QRTFQTRCL*HRQRAKTDTYNLKQNH*LLQHRTVVETLF*WHASGCQKKQHNQDHI*NHL 359
Query: 403 QV**PLHQN*IS*LV**VQMSLLA*FSLCDQHSPGQLVGKIDQLHLPQDCQNSP*RQFRH 224
QV *PLHQ IS*LV +M L A*FSLCD HS QL +IDQ HL + Q+S +QFRH
Sbjct: 358 QVG*PLHQKRIS*LVESARMPLPA*FSLCD*HSLEQLGERIDQQHLHPNSQSSLCQQFRH 179
Query: 223 LCLEHH*QDSEVSQSAQSYRLHPCHICLIMGGQHL*I*LSSLSVKQRQNR 74
LC EHH*QDSEVSQSA SYRLHP IC IMG Q + I* SSL KQR +R
Sbjct: 178 LCQEHH*QDSEVSQSALSYRLHPYRICQIMGAQQILI**SSLREKQR*DR 29
Score = 221 bits (477), Expect = 6e-057
Identities = 114/164 (69%), Positives = 122/164 (74%)
Frame = +2 / +2
Query: 74 SILPLFHR**RQSNL*VLAPHYQADMTWVQSV*LSTLRNFRILLMML*TKVPKLPSGGVL 253
SILPLF **R SN +L PHY AD WVQSV* STLR+FRILLMML TKVPKL + G L
Sbjct: 29 SILPLFLA**RSSNQYLLGPHYLADTIWVQSV**STLRDFRILLMMLLTKVPKLLAEGAL 208
Query: 254 AILGKMQLINFSHQLAW*MLITQ*KLCKKRHLDLLYQS*NSVLMKRLSNLQTTQNMVLAV 433
I KM LIN QL *MLITQ*KLC+KRH YQS*NS+LMKR SNLQ NMVL V
Sbjct: 209 GIWVKMLLINSFPQLF**MLITQ*KLCRKRHSGRFYQS*NSLLMKRSSNLQMILNMVLVV 388
Query: 434 LFFRATRSVPSE*RPSYTVG*QQSMILLQVTCASLCHSVVLKTA 565
LFF ATRSVPS+*R +Y+ QQSMILLQV C SLC VLKTA
Sbjct: 389 LFFLATRSVPSK*RLNYSAVLQQSMILLQVICVSLCPLAVLKTA 520
Score = 151 bits (325), Expect(2) = 6e-050
Identities = 66/99 (66%), Positives = 70/99 (70%)
Frame = +3 / +3
Query: 246 EFWQSWGRCS*SIFPTNWPGEC*SHNENYARRDIWTYYTNHEIQF**RGYQTCKRLKIWS 425
E W+ RC *SI N EC*SHNENYA R I TNHEI F**RG+ TCK IWS
Sbjct: 201 ELWEFG*RCC*SILSPNCSSEC*SHNENYAGRGIRADSTNHEILF**RGHPTCK*F*IWS 380
Query: 426 WLCCFFGQPEACHQNSVPVTLWGSSNQ*FCFKLHVPVFA 542
WLCCFF QPEACHQNSV T+ SNQ*FCFKL+V VFA
Sbjct: 381 WLCCFFWQPEACHQNSVSTTVRCCSNQ*FCFKLYVSVFA 497
Score = 66.6 bits (139), Expect(2) = 6e-050
Identities = 30/45 (66%), Positives = 34/45 (75%)
Frame = +3 / +3
Query: 63 SR*HLFCLCFTDSEDSQIYKCWPPIIRQI*HGCNLYD*AL*ETSE 197
S+ +L LCF+ SED QI CW PII QI +GCNLYD AL*ETSE
Sbjct: 18 SQGYLSYLCFSRSEDHQINICWAPIIWQIRYGCNLYDRAL*ETSE 152
>gb|BQ758765.1|BQ758765 EBma07_SQ002_B22_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ002_B22 5', mRNA sequence
Length = 546
Score = 171 bits (369), Expect = 5e-042
Identities = 67/80 (83%), Positives = 76/80 (95%)
Frame = +1 / +2
Query: 565 GFGRFAGVEGLRACCLVKSVVEDRLWPYIRTVIPKPIQYPVSEHGFEFQQLLVETLYGYS 744
GFGRFAGVEGLRACCLVK+VVEDR WPY++T+IPKPIQYPVSE+GF FQQLLVETLYG S
Sbjct: 2 GFGRFAGVEGLRACCLVKAVVEDRWWPYVKTMIPKPIQYPVSENGFAFQQLLVETLYGIS 181
Query: 745 VWDRLRSLVNLIKMVTEQNS 804
VWDRL+SLVNL+KM++EQ S
Sbjct: 182 VWDRLQSLVNLLKMISEQKS 241
Score = 123 bits (263), Expect = 2e-027
Identities = 52/68 (76%), Positives = 56/68 (82%)
Frame = -3 / -1
Query: 797 CSVTIFIRFTRDRNLSHTL*PYRVSTSNCWNSNPCSETGYWIGFGITVLIYGHNLSSTTD 618
CS IF RFT D +LSHTL PYRVSTS+CWN+NP SETGY IG GI VL YGH+LSSTT
Sbjct: 234 CSEIIFNRFTSDCSLSHTLMPYRVSTSSCWNANPFSETGY*IGLGIIVLTYGHHLSSTTA 55
Query: 617 FTRQQARK 594
FTRQQARK
Sbjct: 54 FTRQQARK 31
>gb|BJ467379.1|BJ467379 BJ467379 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26p15 3', mRNA sequence
Length = 455
Score = 133 bits (284), Expect = 2e-030
Identities = 52/65 (80%), Positives = 61/65 (93%)
Frame = +1 / -2
Query: 610 LVKSVVEDRLWPYIRTVIPKPIQYPVSEHGFEFQQLLVETLYGYSVWDRLRSLVNLIKMV 789
LVK+VVEDR WPY++T+IPKPIQYPVSE+GF FQQLLVETLYG SVWDRL+SLVNL+KM+
Sbjct: 454 LVKAVVEDRWWPYVKTMIPKPIQYPVSENGFAFQQLLVETLYGISVWDRLQSLVNLLKMI 275
Query: 790 TEQNS 804
+EQ S
Sbjct: 274 SEQKS 260
Score = 112 bits (239), Expect = 4e-024
Identities = 47/63 (74%), Positives = 51/63 (80%)
Frame = -3 / +3
Query: 797 CSVTIFIRFTRDRNLSHTL*PYRVSTSNCWNSNPCSETGYWIGFGITVLIYGHNLSSTTD 618
CS IF RFT D +LSHTL PYRVSTS+CWN+NP SETGY IG GI VL YGH+LSSTT
Sbjct: 267 CSEIIFNRFTSDCSLSHTLMPYRVSTSSCWNANPFSETGY*IGLGIIVLTYGHHLSSTTA 446
Query: 617 FTR 609
FTR
Sbjct: 447 FTR 455
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 521,223,423
Number of Sequences: 312970
Number of extensions: 7634700
Number of successful extensions: 44577
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 13414836849
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)