TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2655972.2.1
(666 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA026473.1|CA026473 HZ56A06r HZ Hordeum vulgare subsp. v... 150 2e-042
gb|BU977027.1|BU977027 HA10L12r HA Hordeum vulgare subsp. v... 150 2e-042
gb|BU977028.1|BU977028 HA10L12u HA Hordeum vulgare subsp. v... 150 2e-042
>gb|CA026473.1|CA026473 HZ56A06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ56A06
5-PRIME, mRNA sequence
Length = 503
Score = 150 bits (322), Expect(2) = 2e-042
Identities = 63/79 (79%), Positives = 71/79 (89%)
Frame = +2 / +3
Query: 98 LTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKEQSSRLWNLLLSDAVIYIAGSST 277
L HAQD+GVLS K GGGFFVAFSRDQPQKVYVQ KIKEQS+R+WN+L S A IY+AGSST
Sbjct: 12 LKHAQDKGVLSLKEGGGFFVAFSRDQPQKVYVQHKIKEQSARVWNILCSGASIYVAGSST 191
Query: 278 KMPADVTAALEEVICKEGG 334
KMPADVTAALEEV+ ++GG
Sbjct: 192 KMPADVTAALEEVVRQKGG 248
Score = 115 bits (245), Expect = 3e-025
Identities = 56/102 (54%), Positives = 66/102 (64%)
Frame = -1 / -2
Query: 411 YDHVSMLSLPALSRSLSHFEASACLTPPSLQITSSSAAVTSAGIFVEEPAM*ITASDSNK 232
+D+ +S+ L S +PP + TSSSAAVTSAGI VE+PA I A + N
Sbjct: 325 HDNHDQVSMLNLPALSSFLRHPEAASPPFWRTTSSSAAVTSAGILVEDPATYIEAPEHNM 146
Query: 231 FHSLELCSFILGCT*TFCG*SREKATKKPPPLFEDNTPSSCA 106
FH+L LCSFIL CT TF G*S EKATKKPPP F D+TP S A
Sbjct: 145 FHTLALCSFILCCTYTF*G*SLEKATKKPPPSFRDSTPLSWA 20
Score = 95.5 bits (202), Expect(2) = 6e-025
Identities = 48/83 (57%), Positives = 54/83 (65%)
Frame = +3 / +1
Query: 87 RISG*LMRRMRGCCPRKGAVVSSSLFPGISRKRSTCNPR*RNRVQDCGTYYCPMQ*STLQ 266
R G* M R R CCP K AVVS LFPGIS KR N R*R+RV++CGTYY TLQ
Sbjct: 1 RHEG*SMPRTRECCP*KRAVVSLLLFPGISLKRCMYNTR*RSRVRECGTYYALGLQYTLQ 180
Query: 267 ALRRKCLPMLQLR*RRLFVKKVV 335
L KCL MLQL *++L K+ V
Sbjct: 181 GLLPKCLLMLQLH*KKLSAKRAV 249
Score = 65.2 bits (136), Expect(3) = 7e-021
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = -3 / -1
Query: 232 VPQS*TLFLYLGLHVDLLRLIPGKSDEETTAPFRGQHPLILRMS*P 95
VP S TL LYL L++ LLRLIPGKS++ETTA F GQH L+L M P
Sbjct: 146 VPHSRTLLLYLVLYIHLLRLIPGKSNKETTALF*GQHSLVLGMLQP 9
Score = 41.8 bits (85), Expect(2) = 2e-042
Identities = 17/22 (77%), Positives = 18/22 (81%)
Frame = +2 / +3
Query: 350 ASKWLRDLERAGRLNIETWS*L 415
AS W R LERAG+ NIETWS*L
Sbjct: 255 ASGWRRKLERAGKFNIETWS*L 320
Score = 38.2 bits (77), Expect(2) = 6e-025
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = +1 / +2
Query: 349 CFEMAQGSRKGW*T*HRDMVIVVMGKK 429
CF MAQ + KGW* *H ++V++VM +
Sbjct: 254 CFRMAQETGKGW*I*H*NLVVIVMSNR 334
Score = 38.2 bits (77), Expect(3) = 7e-021
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = -1 / -2
Query: 420 HHNYDHVSMLSLPALSRSLSHFEASA 343
H N+D VSML+LPALS L H EA++
Sbjct: 325 HDNHDQVSMLNLPALSSFLRHPEAAS 248
Score = 34.1 bits (68), Expect(3) = 7e-021
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -3 / -1
Query: 301 SCNIGRHFRRRACNV 257
SCNI RHF RR CNV
Sbjct: 215 SCNISRHFGRRPCNV 171
>gb|BU977027.1|BU977027 HA10L12r HA Hordeum vulgare subsp. vulgare cDNA clone HA10L12
5-PRIME, mRNA sequence
Length = 472
Score = 150 bits (322), Expect(2) = 2e-042
Identities = 63/79 (79%), Positives = 71/79 (89%)
Frame = +2 / +3
Query: 98 LTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKEQSSRLWNLLLSDAVIYIAGSST 277
L HAQD+GVLS K GGGFFVAFSRDQPQKVYVQ KIKEQS+R+WN+L S A IY+AGSST
Sbjct: 12 LKHAQDKGVLSLKEGGGFFVAFSRDQPQKVYVQHKIKEQSARVWNILCSGASIYVAGSST 191
Query: 278 KMPADVTAALEEVICKEGG 334
KMPADVTAALEEV+ ++GG
Sbjct: 192 KMPADVTAALEEVVRQKGG 248
Score = 115 bits (245), Expect = 3e-025
Identities = 56/102 (54%), Positives = 66/102 (64%)
Frame = -1 / -1
Query: 411 YDHVSMLSLPALSRSLSHFEASACLTPPSLQITSSSAAVTSAGIFVEEPAM*ITASDSNK 232
+D+ +S+ L S +PP + TSSSAAVTSAGI VE+PA I A + N
Sbjct: 325 HDNHDQVSMLNLPALSSFLRHPEAASPPFWRTTSSSAAVTSAGILVEDPATYIEAPEHNM 146
Query: 231 FHSLELCSFILGCT*TFCG*SREKATKKPPPLFEDNTPSSCA 106
FH+L LCSFIL CT TF G*S EKATKKPPP F D+TP S A
Sbjct: 145 FHTLALCSFILCCTYTF*G*SLEKATKKPPPSFRDSTPLSWA 20
Score = 95.5 bits (202), Expect(2) = 6e-025
Identities = 48/83 (57%), Positives = 54/83 (65%)
Frame = +3 / +1
Query: 87 RISG*LMRRMRGCCPRKGAVVSSSLFPGISRKRSTCNPR*RNRVQDCGTYYCPMQ*STLQ 266
R G* M R R CCP K AVVS LFPGIS KR N R*R+RV++CGTYY TLQ
Sbjct: 1 RHEG*SMPRTRECCP*KRAVVSLLLFPGISLKRCMYNTR*RSRVRECGTYYALGLQYTLQ 180
Query: 267 ALRRKCLPMLQLR*RRLFVKKVV 335
L KCL MLQL *++L K+ V
Sbjct: 181 GLLPKCLLMLQLH*KKLSAKRAV 249
Score = 65.2 bits (136), Expect(3) = 7e-021
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = -3 / -3
Query: 232 VPQS*TLFLYLGLHVDLLRLIPGKSDEETTAPFRGQHPLILRMS*P 95
VP S TL LYL L++ LLRLIPGKS++ETTA F GQH L+L M P
Sbjct: 146 VPHSRTLLLYLVLYIHLLRLIPGKSNKETTALF*GQHSLVLGMLQP 9
Score = 41.8 bits (85), Expect(2) = 2e-042
Identities = 17/22 (77%), Positives = 18/22 (81%)
Frame = +2 / +3
Query: 350 ASKWLRDLERAGRLNIETWS*L 415
AS W R LERAG+ NIETWS*L
Sbjct: 255 ASGWRRKLERAGKFNIETWS*L 320
Score = 38.2 bits (77), Expect(2) = 6e-025
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = +1 / +2
Query: 349 CFEMAQGSRKGW*T*HRDMVIVVMGKK 429
CF MAQ + KGW* *H ++V++VM +
Sbjct: 254 CFRMAQETGKGW*I*H*NLVVIVMSNR 334
Score = 38.2 bits (77), Expect(3) = 7e-021
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = -1 / -1
Query: 420 HHNYDHVSMLSLPALSRSLSHFEASA 343
H N+D VSML+LPALS L H EA++
Sbjct: 325 HDNHDQVSMLNLPALSSFLRHPEAAS 248
Score = 34.1 bits (68), Expect(3) = 7e-021
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -3 / -3
Query: 301 SCNIGRHFRRRACNV 257
SCNI RHF RR CNV
Sbjct: 215 SCNISRHFGRRPCNV 171
>gb|BU977028.1|BU977028 HA10L12u HA Hordeum vulgare subsp. vulgare cDNA clone HA10L12
3-PRIME, mRNA sequence
Length = 472
Score = 150 bits (322), Expect(2) = 2e-042
Identities = 63/79 (79%), Positives = 71/79 (89%)
Frame = +2 / -3
Query: 98 LTHAQDEGVLSSKRGGGFFVAFSRDQPQKVYVQPKIKEQSSRLWNLLLSDAVIYIAGSST 277
L HAQD+GVLS K GGGFFVAFSRDQPQKVYVQ KIKEQS+R+WN+L S A IY+AGSST
Sbjct: 461 LKHAQDKGVLSLKEGGGFFVAFSRDQPQKVYVQHKIKEQSARVWNILCSGASIYVAGSST 282
Query: 278 KMPADVTAALEEVICKEGG 334
KMPADVTAALEEV+ ++GG
Sbjct: 281 KMPADVTAALEEVVRQKGG 225
Score = 115 bits (245), Expect = 3e-025
Identities = 56/102 (54%), Positives = 66/102 (64%)
Frame = -1 / +1
Query: 411 YDHVSMLSLPALSRSLSHFEASACLTPPSLQITSSSAAVTSAGIFVEEPAM*ITASDSNK 232
+D+ +S+ L S +PP + TSSSAAVTSAGI VE+PA I A + N
Sbjct: 148 HDNHDQVSMLNLPALSSFLRHPEAASPPFWRTTSSSAAVTSAGILVEDPATYIEAPEHNM 327
Query: 231 FHSLELCSFILGCT*TFCG*SREKATKKPPPLFEDNTPSSCA 106
FH+L LCSFIL CT TF G*S EKATKKPPP F D+TP S A
Sbjct: 328 FHTLALCSFILCCTYTF*G*SLEKATKKPPPSFRDSTPLSWA 453
Score = 95.5 bits (202), Expect(2) = 6e-025
Identities = 48/83 (57%), Positives = 54/83 (65%)
Frame = +3 / -1
Query: 87 RISG*LMRRMRGCCPRKGAVVSSSLFPGISRKRSTCNPR*RNRVQDCGTYYCPMQ*STLQ 266
R G* M R R CCP K AVVS LFPGIS KR N R*R+RV++CGTYY TLQ
Sbjct: 472 RHEG*SMPRTRECCP*KRAVVSLLLFPGISLKRCMYNTR*RSRVRECGTYYALGLQYTLQ 293
Query: 267 ALRRKCLPMLQLR*RRLFVKKVV 335
L KCL MLQL *++L K+ V
Sbjct: 292 GLLPKCLLMLQLH*KKLSAKRAV 224
Score = 65.2 bits (136), Expect(3) = 7e-021
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = -3 / +3
Query: 232 VPQS*TLFLYLGLHVDLLRLIPGKSDEETTAPFRGQHPLILRMS*P 95
VP S TL LYL L++ LLRLIPGKS++ETTA F GQH L+L M P
Sbjct: 327 VPHSRTLLLYLVLYIHLLRLIPGKSNKETTALF*GQHSLVLGMLQP 464
Score = 41.8 bits (85), Expect(2) = 2e-042
Identities = 17/22 (77%), Positives = 18/22 (81%)
Frame = +2 / -3
Query: 350 ASKWLRDLERAGRLNIETWS*L 415
AS W R LERAG+ NIETWS*L
Sbjct: 218 ASGWRRKLERAGKFNIETWS*L 153
Score = 38.2 bits (77), Expect(2) = 6e-025
Identities = 14/27 (51%), Positives = 20/27 (74%)
Frame = +1 / -2
Query: 349 CFEMAQGSRKGW*T*HRDMVIVVMGKK 429
CF MAQ + KGW* *H ++V++VM +
Sbjct: 219 CFRMAQETGKGW*I*H*NLVVIVMSNR 139
Score = 38.2 bits (77), Expect(3) = 7e-021
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = -1 / +1
Query: 420 HHNYDHVSMLSLPALSRSLSHFEASA 343
H N+D VSML+LPALS L H EA++
Sbjct: 148 HDNHDQVSMLNLPALSSFLRHPEAAS 225
Score = 34.1 bits (68), Expect(3) = 7e-021
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -3 / +3
Query: 301 SCNIGRHFRRRACNV 257
SCNI RHF RR CNV
Sbjct: 258 SCNISRHFGRRPCNV 302
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 329,469,408
Number of Sequences: 312970
Number of extensions: 5482135
Number of successful extensions: 57339
Number of sequences better than 1.0e-020: 3
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7210840530
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)