TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2623104.2.4
(1628 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow st... 166 2e-072
gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow st... 127 6e-058
gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow st... 224 1e-057
gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow st... 127 1e-049
gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow st... 122 4e-048
gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow st... 176 3e-043
gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow st... 138 3e-041
gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow st... 127 3e-037
gb|BQ464404.1|BQ464404 HF02C24r HF Hordeum vulgare subsp. v... 143 3e-033
gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. v... 136 3e-031
gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachi... 117 1e-025
>gb|BM442515.2|BM442515 EBan01_SQ003_G09_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_G09 5', mRNA sequence
Length = 588
Score = 166 bits (357), Expect(2) = 2e-072
Identities = 67/90 (74%), Positives = 74/90 (82%)
Frame = +2 / +3
Query: 779 GVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKIHY 958
GVTCGPGHGISIGSLGRYKDEKDVTDI+VK+C K + G+RIK+YED+ S L SKI Y
Sbjct: 3 GVTCGPGHGISIGSLGRYKDEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISY 182
Query: 959 ENIKMEDSANPIFIDMKYCPNKLCTANGAS 1048
ENIKM+DS PI ID KYCPNKLCTA G S
Sbjct: 183 ENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 272
Score = 132 bits (283), Expect(2) = 9e-056
Identities = 61/90 (67%), Positives = 68/90 (75%)
Frame = -3 / -3
Query: 1047 EAPLAVHNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTPNIVFLRVQS 868
E+PLAV +LLGQYF SM MG ESSIL+FS* IL+ + SSY LMR P + L Q
Sbjct: 271 ESPLAVQSLLGQYF*SMMMG*PESSILMFS*LILDATRGDLESSYDLMRRPLVDPLNTQF 92
Query: 867 LTLMSVTSFSSLYRPRLPMLMPWPGPQVTP 778
LT MSVTSFSSLY PRLP+LMPWPGPQVTP
Sbjct: 91 LTWMSVTSFSSLYLPRLPILMPWPGPQVTP 2
Score = 127 bits (273), Expect(2) = 2e-072
Identities = 53/73 (72%), Positives = 59/73 (80%)
Frame = +2 / +3
Query: 1043 ASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNA 1222
+S+VTVKDVTFKNITGTSSTPEAVSLLC+ K PC GVTM DV +EYSG NNKTMA+CTN
Sbjct: 270 SSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVCTNV 449
Query: 1223 KGSTKGCLKELAC 1261
K + G K AC
Sbjct: 450 KVTATGVDKANAC 488
Score = 105 bits (225), Expect(2) = 9e-056
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -3 / -3
Query: 1260 HASSLRQPLVLPLAFVHIAMVLLLVPLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVM 1081
HA +L P+ + L V AMVLL +P YS LTS +VTP G L+ QSRLTASGVEEVPVM
Sbjct: 487 HALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPSQGFLSEQSRLTASGVEEVPVM 308
Query: 1080 FLKVTSLTVTLE 1045
FLKVTSLTVTLE
Sbjct: 307 FLKVTSLTVTLE 272
>gb|BQ764168.1|BQ764168 EBan01_SQ005_F02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ005_F02 5', mRNA sequence
Length = 693
Score = 127 bits (273), Expect(2) = 6e-058
Identities = 53/73 (72%), Positives = 59/73 (80%)
Frame = +2 / +2
Query: 1043 ASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNA 1222
+S+VTVKDVTFKNITGTSSTPEAVSLLC+ K PC GVTM DV +EYSG NNKTMA+CTN
Sbjct: 212 SSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVCTNV 391
Query: 1223 KGSTKGCLKELAC 1261
K + G K AC
Sbjct: 392 KVTATGVDKANAC 430
Score = 117 bits (251), Expect(2) = 6e-058
Identities = 48/71 (67%), Positives = 55/71 (77%)
Frame = +2 / +2
Query: 836 DEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYC 1015
DEKDVTDI+VK+C K + G+RIK+YED+ S L SKI YENIKM+DS PI ID KYC
Sbjct: 2 DEKDVTDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYC 181
Query: 1016 PNKLCTANGAS 1048
PNKLCTA G S
Sbjct: 182 PNKLCTAKGDS 214
Score = 105 bits (225), Expect(2) = 6e-042
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -3 / -1
Query: 1260 HASSLRQPLVLPLAFVHIAMVLLLVPLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVM 1081
HA +L P+ + L V AMVLL +P YS LTS +VTP G L+ QSRLTASGVEEVPVM
Sbjct: 429 HALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPSQGFLSEQSRLTASGVEEVPVM 250
Query: 1080 FLKVTSLTVTLE 1045
FLKVTSLTVTLE
Sbjct: 249 FLKVTSLTVTLE 214
Score = 86.3 bits (182), Expect(2) = 6e-042
Identities = 44/71 (61%), Positives = 50/71 (70%)
Frame = -3 / -1
Query: 1047 EAPLAVHNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTPNIVFLRVQS 868
E+PLAV +LLGQYF SM MG ESSIL+FS* IL+ + SSY LMR P + L Q
Sbjct: 213 ESPLAVQSLLGQYF*SMMMG*PESSILMFS*LILDATRGDLESSYDLMRRPLVDPLNTQF 34
Query: 867 LTLMSVTSFSS 835
LT MSVTSFSS
Sbjct: 33 LTWMSVTSFSS 1
>gb|BM442620.2|BM442620 EBan01_SQ003_L02_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_L02 5', mRNA sequence
Length = 377
Score = 224 bits (483), Expect = 1e-057
Identities = 89/124 (71%), Positives = 103/124 (83%)
Frame = +2 / +2
Query: 671 GIHMGDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDV 850
GIHMGDSS + I +T I VGDDCISIGPG+ +VNI+GVTCGP HGISIGSLGRYKDEKDV
Sbjct: 2 GIHMGDSSNVNIIDTTIAVGDDCISIGPGSKQVNISGVTCGPSHGISIGSLGRYKDEKDV 181
Query: 851 TDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLC 1030
TDI+VK+C K + G+RIK+YED+ S L SKI YENIKM+DS+ PI ID KYCP+KLC
Sbjct: 182 TDIHVKNCVFKGSTNGLRIKSYEDSKSPLVASKISYENIKMDDSSYPIIIDQKYCPHKLC 361
Query: 1031 TANG 1042
TA G
Sbjct: 362 TAKG 373
Score = 179 bits (386), Expect = 3e-044
Identities = 82/125 (65%), Positives = 92/125 (73%)
Frame = -3 / -3
Query: 1044 APLAVHNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTPNIVFLRVQSL 865
+PLAV +L GQYF SM MG ESSIL+FS* IL+ + SSY LMR P + L Q L
Sbjct: 375 SPLAVQSLWGQYF*SMMMG*LESSILMFS*LILDATRGDLESSYDLMRRPLVDPLNTQFL 196
Query: 864 TLMSVTSFSSLYRPRLPMLMPWPGPQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVIPDES 685
T MSVTSFSSLY PRLP+LMPW GPQVTP+M T PGPMEMQSSPT M V +M+ DES
Sbjct: 195 TWMSVTSFSSLYLPRLPILMPWLGPQVTPLMLTCLEPGPMEMQSSPTAMVVSMMLTLDES 16
Query: 684 PMWMP 670
PMWMP
Sbjct: 15 PMWMP 1
>gb|BQ753595.1|BQ753595 EBan01_SQ004_L07_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_L07 5', mRNA sequence
Length = 436
Score = 127 bits (273), Expect(2) = 1e-049
Identities = 53/73 (72%), Positives = 59/73 (80%)
Frame = +2 / +2
Query: 1043 ASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNA 1222
+S+VTVKDVTFKNITGTSSTPEAVSLLC+ K PC GVTM DV +EYSG NNKTMA+CTN
Sbjct: 152 SSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVCTNV 331
Query: 1223 KGSTKGCLKELAC 1261
K + G K AC
Sbjct: 332 KVTATGVDKANAC 370
Score = 105 bits (225), Expect(2) = 5e-036
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -3 / -2
Query: 1260 HASSLRQPLVLPLAFVHIAMVLLLVPLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVM 1081
HA +L P+ + L V AMVLL +P YS LTS +VTP G L+ QSRLTASGVEEVPVM
Sbjct: 369 HALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPSQGFLSEQSRLTASGVEEVPVM 190
Query: 1080 FLKVTSLTVTLE 1045
FLKVTSLTVTLE
Sbjct: 189 FLKVTSLTVTLE 154
Score = 90.0 bits (190), Expect(2) = 1e-049
Identities = 36/51 (70%), Positives = 40/51 (78%)
Frame = +2 / +2
Query: 896 GVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGAS 1048
G+RIK+YED+ S L SKI YENIKM+DS PI ID KYCPNKLCTA G S
Sbjct: 2 GLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 154
Score = 66.6 bits (139), Expect(2) = 5e-036
Identities = 32/51 (62%), Positives = 37/51 (72%)
Frame = -3 / -2
Query: 1047 EAPLAVHNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTP 895
E+PLAV +LLGQYF SM MG ESSIL+FS* IL+ + SSY LMR P
Sbjct: 153 ESPLAVQSLLGQYF*SMMMG*PESSILMFS*LILDATRGDLESSYDLMRRP 1
>gb|BM442473.2|BM442473 EBan01_SQ003_E14_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ003_E14 5', mRNA sequence
Length = 454
Score = 122 bits (262), Expect(2) = 4e-048
Identities = 52/73 (71%), Positives = 58/73 (79%)
Frame = +2 / +3
Query: 1043 ASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNA 1222
+S+VTVKDVTFKNITGTSSTPEAVSLLC+ K P GVTM DV +EYSG NNKTMA+CTN
Sbjct: 177 SSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPYEGVTMSDVKIEYSGKNNKTMAVCTNV 356
Query: 1223 KGSTKGCLKELAC 1261
K + G K AC
Sbjct: 357 KVTATGVDKANAC 395
Score = 104 bits (221), Expect(2) = 2e-035
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -3 / -1
Query: 1260 HASSLRQPLVLPLAFVHIAMVLLLVPLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVM 1081
HA +L P+ + L V AMVLL +P YS LTS +VTP G L+ QSRLTASGVEEVPVM
Sbjct: 394 HALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPS*GFLSEQSRLTASGVEEVPVM 215
Query: 1080 FLKVTSLTVTLE 1045
FLKVTSLTVTLE
Sbjct: 214 FLKVTSLTVTLE 179
Score = 90.0 bits (190), Expect(2) = 4e-048
Identities = 36/51 (70%), Positives = 40/51 (78%)
Frame = +2 / +3
Query: 896 GVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGAS 1048
G+RIK+YED+ S L SKI YENIKM+DS PI ID KYCPNKLCTA G S
Sbjct: 27 GLRIKSYEDSKSPLVASKISYENIKMDDSGYPIIIDQKYCPNKLCTAKGDS 179
Score = 66.6 bits (139), Expect(2) = 2e-035
Identities = 32/51 (62%), Positives = 37/51 (72%)
Frame = -3 / -1
Query: 1047 EAPLAVHNLLGQYFMSMKMGLAESSILIFS*WILETVSTEAASSYALMRTP 895
E+PLAV +LLGQYF SM MG ESSIL+FS* IL+ + SSY LMR P
Sbjct: 178 ESPLAVQSLLGQYF*SMMMG*PESSILMFS*LILDATRGDLESSYDLMRRP 26
>gb|BM442145.2|BM442145 EBan01_SQ002_F12_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_F12 5', mRNA sequence
Length = 311
Score = 176 bits (379), Expect = 3e-043
Identities = 65/101 (64%), Positives = 84/101 (83%)
Frame = +2 / +3
Query: 419 NLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMN 598
NLVITGKG++D QG AVW+KNSC K Y+CKILPNSLV+DF ++ + ++++NSKFFHMN
Sbjct: 6 NLVITGKGSIDRQGKAVWTKNSCQKNYNCKILPNSLVLDFCDDALIESISIINSKFFHMN 185
Query: 599 MYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVI 721
+Y CK +++KDV V+APGDSPNTDGIHMGDS + I +T I
Sbjct: 186 IYECKGVIVKDVKVSAPGDSPNTDGIHMGDSFNVNIIDTTI 308
Score = 115 bits (246), Expect = 6e-025
Identities = 58/100 (58%), Positives = 64/100 (64%)
Frame = -3 / -2
Query: 720 MTVLVMVIPDESPMWMPSVLGLSPGAVTVTSLISMSLHRYMFMWKNLELSSVTPDTSPLF 541
M V +M+ +ESPMWMPSVLGLSPGA+T TSL LH MFMWKNLEL S
Sbjct: 307 MVVSMMLTLNESPMWMPSVLGLSPGALTFTSLTITPLHS*MFMWKNLELMIEMLSMSASS 128
Query: 540 TKSITSELGRILQSYFLVQEFLLHTAGPCPSRFPLPVMTR 421
KS T E G+ILQ F Q FL+ TA P S PLPVMTR
Sbjct: 127 QKSSTKEFGKILQL*FFWQLFLVQTALP*RSIDPLPVMTR 8
Score = 89.0 bits (188), Expect(2) = 8e-021
Identities = 40/85 (47%), Positives = 48/85 (56%)
Frame = -2 / -1
Query: 670 IRVGAVPGRRHGHVLDQHVLAPVHVHVEELGVEQRDPGHLPVVHEVHHQRVGKDXXXXXX 491
+ VGAVPGR H HV D H A V VHVEELG++ RD V+ EV HQ + +D
Sbjct: 257 VGVGAVPGRAHFHVFDDHAFALVDVHVEELGIDDRDAFDERVITEVQHQGIWQDLAVVVL 78
Query: 490 XXXXXAPHGWALPVKVSLAGDDQVI 416
P G AL V S AGDD+V+
Sbjct: 77 LATVLGPDGLALTVDRSFAGDDEVL 3
Score = 32.2 bits (64), Expect(2) = 8e-021
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = -1 / -3
Query: 689 SRPCGCHPCWG 657
SRPCGC CWG
Sbjct: 276 SRPCGCRRCWG 244
>gb|BQ753380.1|BQ753380 EBan01_SQ004_B18_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ004_B18 5', mRNA sequence
Length = 431
Score = 138 bits (296), Expect(4) = 3e-041
Identities = 54/64 (84%), Positives = 58/64 (90%)
Frame = +2 / +1
Query: 134 ASGPGGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGP 313
ASGPGG +DI KLGA +GKTDSTKAV+EAWASACGGTGKQTI+IPKGDFL G LNFTGP
Sbjct: 136 ASGPGGEYDIIKLGAKPDGKTDSTKAVEEAWASACGGTGKQTIVIPKGDFLTGALNFTGP 315
Query: 314 CKGD 325
CKGD
Sbjct: 316 CKGD 327
Score = 113 bits (241), Expect(3) = 2e-027
Identities = 47/70 (67%), Positives = 52/70 (74%)
Frame = -3 / -1
Query: 342 STWMVTSPLQGPVKLSCPTRKSPLGMRIVCFPVPPHADAHASCTAFVLSVLPLPEAPNLV 163
S + SPL GPVK P RKSPLGM IVC PVPPHA+AHAS TA VLSVLP AP+L+
Sbjct: 344 SLMVTPSPLHGPVKFRAPVRKSPLGMTIVCLPVPPHAEAHASSTALVLSVLPSGFAPSLM 165
Query: 162 MSKDPPGPDA 133
MS PPGP+A
Sbjct: 164 MSYSPPGPEA 135
Score = 29.9 bits (59), Expect(4) = 3e-041
Identities = 11/18 (61%), Positives = 16/18 (88%)
Frame = +2 / +1
Query: 326 VTIQVDGNLLATTDLSQY 379
VTI+++GNLLA+ DLS +
Sbjct: 331 VTIKLEGNLLASNDLSMF 384
Score = 29.0 bits (57), Expect(4) = 3e-041
Identities = 10/13 (76%), Positives = 12/13 (92%)
Frame = +2 / +1
Query: 392 NWIEILRVDNLVI 430
NWIEI+R+ NLVI
Sbjct: 391 NWIEIMRIKNLVI 429
Score = 28.1 bits (55), Expect(4) = 3e-041
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = +2 / +1
Query: 29 DKMACTNNAMRALFLLVLFCIVHGEKEESK 118
D+MA N AMR L L+V+ + + +E K
Sbjct: 7 DRMAFKNVAMRVLLLMVVVSAAYAKGKEEK 96
Score = 24.0 bits (46), Expect(3) = 2e-027
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -3 / -1
Query: 78 TRRNKALIALFVHAILS 28
T N+ LIA F++AILS
Sbjct: 56 TMSNRTLIATFLNAILS 6
Score = 24.0 bits (46), Expect(3) = 2e-027
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = -3 / -1
Query: 369 RSVVASRLPSTWMVT 325
RS+ AS+LPS+ MVT
Sbjct: 374 RSLEASKLPSSLMVT 330
>gb|BQ753283.1|BQ753283 EBan01_SQ001_C11_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ001_C11 5', mRNA sequence
Length = 647
Score = 127 bits (273), Expect(2) = 3e-037
Identities = 53/73 (72%), Positives = 59/73 (80%)
Frame = +2 / +1
Query: 1043 ASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNA 1222
+S+VTVKDVTFKNITGTSSTPEAVSLLC+ K PC GVTM DV +EYSG NNKTMA+CTN
Sbjct: 73 SSRVTVKDVTFKNITGTSSTPEAVSLLCSDKKPCEGVTMSDVKIEYSGKNNKTMAVCTNV 252
Query: 1223 KGSTKGCLKELAC 1261
K + G K AC
Sbjct: 253 KVTATGVDKANAC 291
Score = 105 bits (225), Expect(2) = 1e-027
Identities = 49/72 (68%), Positives = 55/72 (76%)
Frame = -3 / -1
Query: 1260 HASSLRQPLVLPLAFVHIAMVLLLVPLYSTLTSSMVTPVHGTLAVQSRLTASGVEEVPVM 1081
HA +L P+ + L V AMVLL +P YS LTS +VTP G L+ QSRLTASGVEEVPVM
Sbjct: 290 HALALSTPVAVTLTLVQTAMVLLFLPEYSILTSDIVTPSQGFLSEQSRLTASGVEEVPVM 111
Query: 1080 FLKVTSLTVTLE 1045
FLKVTSLTVTLE
Sbjct: 110 FLKVTSLTVTLE 75
Score = 48.7 bits (100), Expect(2) = 3e-037
Identities = 18/25 (72%), Positives = 19/25 (76%)
Frame = +2 / +1
Query: 974 EDSANPIFIDMKYCPNKLCTANGAS 1048
+DS PI ID KYCPNKLCTA G S
Sbjct: 1 DDSGYPIIIDQKYCPNKLCTAKGDS 75
Score = 38.2 bits (77), Expect(2) = 1e-027
Identities = 17/24 (70%), Positives = 19/24 (79%)
Frame = -3 / -1
Query: 1047 EAPLAVHNLLGQYFMSMKMGLAES 976
E+PLAV +LLGQYF SM MG ES
Sbjct: 74 ESPLAVQSLLGQYF*SMMMG*PES 3
>gb|BQ464404.1|BQ464404 HF02C24r HF Hordeum vulgare subsp. vulgare cDNA clone HF02C24
5-PRIME, mRNA sequence
Length = 579
Score = 143 bits (306), Expect = 3e-033
Identities = 58/138 (42%), Positives = 91/138 (65%)
Frame = +2 / +2
Query: 506 KILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMG 685
++ P +L +N V+G+T++NS H+ C+ +L+ D+T+++P +S NTDGIH+
Sbjct: 152 RVKPTALRFYGSSNVVVAGITIVNSSQCHLKFDNCQGVLVHDLTISSPENSLNTDGIHLQ 331
Query: 686 DSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINV 865
+S ++I +T + GDDCISI G S + I V CGPGHGISIG LGR + V+++ V
Sbjct: 332 NSKDVSIHHTNLACGDDCISIQTGCSNIYIHNVNCGPGHGISIGGLGRDNTKACVSNVTV 511
Query: 866 KDCTLKKTMFGVRIKAYE 919
+D + +TM GVRIK ++
Sbjct: 512 RDVNMFRTMTGVRIKTWQ 565
>gb|CB881820.1|CB881820 HM10P12w HM Hordeum vulgare subsp. vulgare cDNA clone HM10P12
3-PRIME, mRNA sequence
Length = 608
Score = 136 bits (292), Expect = 3e-031
Identities = 55/142 (38%), Positives = 83/142 (58%)
Frame = +2 / +3
Query: 503 CKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHM 682
CK P ++ +D V G+T+ N++ H+ + R + + + + + APGDSPNTDGIH+
Sbjct: 81 CKGAPTAVTIDSCRGVRVRGLTIQNAQQMHLTVSRSRGVRLDGMAIQAPGDSPNTDGIHV 260
Query: 683 GDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDIN 862
+S+ +TIT IG GDDC+SI + V + G+ C PGHGISIGSLG+ V +
Sbjct: 261 AESTAVTITGARIGTGDDCVSISNASFAVKMKGIVCDPGHGISIGSLGQGGSYAAVEGVT 440
Query: 863 VKDCTLKKTMFGVRIKAYEDAA 928
+ + + GVRIK + A
Sbjct: 441 LDGARIARAQNGVRIKTXQGGA 506
Score = 90.4 bits (191), Expect(2) = 1e-021
Identities = 37/61 (60%), Positives = 42/61 (68%)
Frame = -3 / -2
Query: 825 PRLPMLMPWPGPQVTPVMFTLEVPGPMEMQSSPTPMTVLVMVIPDESPMWMPSVLGLSPG 646
PRLPMLMPWPG P +FT ++ M+ QSSP PM VMV +S WMPSVLGLSPG
Sbjct: 403 PRLPMLMPWPGSHTMPFIFTAKLALEMDTQSSPVPMRAPVMVTAVDSATWMPSVLGLSPG 224
Query: 645 A 643
A
Sbjct: 223 A 221
Score = 33.6 bits (67), Expect(2) = 1e-021
Identities = 16/39 (41%), Positives = 18/39 (46%)
Frame = -2 / -1
Query: 670 IRVGAVPGRRHGHVLDQHVLAPVHVHVEELGVEQRDPGH 554
+ VGAVP R GH + H P H V L V R H
Sbjct: 248 VGVGAVPRRLDGHPVQPHPARPRHRQVHLLRVLDRQAAH 132
>gb|BI957299.1|BI957299 HVSMEn0008L17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0008L17f, mRNA sequence
Length = 458
Score = 117 bits (251), Expect = 1e-025
Identities = 48/104 (46%), Positives = 66/104 (63%)
Frame = +2 / +1
Query: 515 PNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSS 694
P +L NN V G+ + NS FH C+ + + +++ +P SPNTDGIH+ ++
Sbjct: 52 PVALRFFMSNNVTVQGLKVQNSPEFHFRFDSCRGVRVYSLSIISPALSPNTDGIHVENTQ 231
Query: 695 GITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLG 826
+ ITNTV+ GD+C+ IG GT V+I VTCGPGHGI IGSLG
Sbjct: 232 DVLITNTVVSNGDNCVCIGAGTLNVHIENVTCGPGHGIIIGSLG 363
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 733,235,682
Number of Sequences: 312970
Number of extensions: 13691036
Number of successful extensions: 149183
Number of sequences better than 1.0e-020: 12
length of database: 58,378,179
effective HSP length: 54
effective length of database: 41,477,799
effective search space used: 20241165912
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)