TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2623051.2.1
(991 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CK125116.1|CK125116 BES1824106l24 BES1824 Hordeum vulgar... 142 4e-054
gb|AL504252.1|AL504252 AL504252 Hordeum vulgare Barke roots... 142 2e-053
gb|BJ453869.1|BJ453869 BJ453869 K. Sato unpublished cDNA li... 142 7e-052
gb|AL504719.1|AL504719 AL504719 Hordeum vulgare Barke roots... 127 2e-049
gb|BI778941.2|BI778941 EBro01_SQ002_C23_R root, 3 week, hyd... 121 9e-042
gb|CA012046.1|CA012046 HT04E15r HT Hordeum vulgare subsp. v... 101 9e-038
gb|BQ766925.1|BQ766925 EBro08_SQ007_B17_R root, 3 week, dro... 91 1e-034
gb|AW982401.2|AW982401 HVSMEg0003C19f Hordeum vulgare pre-a... 144 5e-034
gb|BQ762675.1|BQ762675 EBro02_SQ004_O15 _R root, 3 week, hy... 87 2e-033
gb|CA008912.1|CA008912 HU12I03r HU Hordeum vulgare subsp. v... 124 2e-032
gb|BJ461404.1|BJ461404 BJ461404 K. Sato unpublished cDNA li... 132 4e-030
gb|BQ465184.1|BQ465184 HU02O05r HU Hordeum vulgare subsp. v... 126 2e-028
gb|BF064456.3|BF064456 HV_CEb0015C20f Hordeum vulgare seedl... 116 1e-025
gb|BQ663493.1|BQ663493 HU02O05u HU Hordeum vulgare subsp. v... 112 2e-024
gb|BG344635.2|BG344635 HVSMEg0009O02f Hordeum vulgare pre-a... 69 6e-023
gb|BI951782.1|BI951782 HVSMEm0002M12f Hordeum vulgare green... 102 4e-021
>gb|CK125116.1|CK125116 BES1824106l24 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010L246 5-PRIME, mRNA sequence
Length = 777
Score = 142 bits (305), Expect(2) = 4e-054
Identities = 55/77 (71%), Positives = 69/77 (89%)
Frame = -2 / +1
Query: 687 EIDVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFF 508
E++V+LIITDYCMPGMTGYDLL ++K SSS DIPVVIMSSEN+P+RI+RCL++GA EFF
Sbjct: 316 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 495
Query: 507 LKPVKPADMKKLKSHLL 457
LKPV+ +DM KLK H++
Sbjct: 496 LKPVRLSDMSKLKPHIM 546
Score = 111 bits (237), Expect(2) = 2e-034
Identities = 50/73 (68%), Positives = 55/73 (75%)
Frame = +2 / -2
Query: 470 LSFFMSAGFTGFRKNSSAPSCKHLLIRAGTFSDDMITTGMSVSDEDPFTRVSRSYPVMPG 649
LS MS TGFRKNS APS +HLLI G FSDDMITTGMS+ D+D +SRSYPV+PG
Sbjct: 533 LSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSYPVIPG 354
Query: 650 MQ*SVMMRLTSIS 688
MQ*SVMMR T S
Sbjct: 353 MQ*SVMMRFTFTS 315
Score = 89.5 bits (189), Expect(2) = 4e-054
Identities = 40/61 (65%), Positives = 46/61 (75%)
Frame = -2 / +1
Query: 933 LRSPAMTVLDAESRFHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGLRDNG 754
L SP AE+ FHVLAVDDS++DRKLIE LLK+SS+QVTTVDSG KAL+ LGL
Sbjct: 97 LSSPMAVAAIAEAPFHVLAVDDSVLDRKLIERLLKTSSFQVTTVDSGTKALQFLGLHAED 276
Query: 753 A 751
A
Sbjct: 277 A 279
Score = 83.5 bits (176), Expect(2) = 4e-022
Identities = 38/61 (62%), Positives = 44/61 (72%)
Frame = +1 / -1
Query: 505 QEELLRAILQAPADPGRHVLRRHDHHRNVRER*GPLHSREQIVSCHAGHAVVSDDETHVD 684
QEEL+ + LQA + GR+VLR HDH+R+V ER L EQIVSCH HAVVSDDE HV
Sbjct: 498 QEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSCHPRHAVVSDDEVHVH 319
Query: 685 L 687
L
Sbjct: 318 L 316
Score = 54.7 bits (113), Expect(2) = 2e-034
Identities = 23/34 (67%), Positives = 26/34 (76%)
Frame = +1 / -1
Query: 799 IHRGHLVRRALEQHLDELPVDDAVVHRQDMEPRL 900
IH G+L RR LE+ LDELPV DAV+H QDME L
Sbjct: 231 IHSGNLERRCLEEPLDELPVKDAVIHGQDMERSL 130
Score = 41.4 bits (84), Expect(2) = 4e-022
Identities = 18/47 (38%), Positives = 22/47 (46%)
Frame = +2 / -2
Query: 794 PESTVVTW*DEXXXXXXXXXXXXXXXXTARTWNRDSASSTVMAGERR 934
PESTVVTW ++ TA+TWN SA + GE R
Sbjct: 236 PESTVVTWNEDVLRSLSMSFLSRTLSSTAKTWNGASAIAATAIGEDR 96
>gb|AL504252.1|AL504252 AL504252 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04L02V 5', mRNA sequence
Length = 700
Score = 142 bits (305), Expect(2) = 2e-053
Identities = 55/77 (71%), Positives = 69/77 (89%)
Frame = -2 / +3
Query: 687 EIDVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFF 508
E++V+LIITDYCMPGMTGYDLL ++K SSS DIPVVIMSSEN+P+RI+RCL++GA EFF
Sbjct: 333 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 512
Query: 507 LKPVKPADMKKLKSHLL 457
LKPV+ +DM KLK H++
Sbjct: 513 LKPVRLSDMSKLKPHIM 563
Score = 111 bits (237), Expect(2) = 4e-033
Identities = 50/73 (68%), Positives = 55/73 (75%)
Frame = +2 / -1
Query: 470 LSFFMSAGFTGFRKNSSAPSCKHLLIRAGTFSDDMITTGMSVSDEDPFTRVSRSYPVMPG 649
LS MS TGFRKNS APS +HLLI G FSDDMITTGMS+ D+D +SRSYPV+PG
Sbjct: 550 LSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSYPVIPG 371
Query: 650 MQ*SVMMRLTSIS 688
MQ*SVMMR T S
Sbjct: 370 MQ*SVMMRFTFTS 332
Score = 87.2 bits (184), Expect(2) = 2e-053
Identities = 39/61 (63%), Positives = 45/61 (73%)
Frame = -2 / +3
Query: 933 LRSPAMTVLDAESRFHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGLRDNG 754
L SP AE+ FHVLAVDDS++DRKLIE LLK+SS+QVTT DSG KAL+ LGL
Sbjct: 114 LSSPMAVAAIAEAPFHVLAVDDSVLDRKLIERLLKTSSFQVTTXDSGTKALQFLGLHAED 293
Query: 753 A 751
A
Sbjct: 294 A 296
Score = 83.5 bits (176), Expect(2) = 2e-021
Identities = 38/61 (62%), Positives = 44/61 (72%)
Frame = +1 / -3
Query: 505 QEELLRAILQAPADPGRHVLRRHDHHRNVRER*GPLHSREQIVSCHAGHAVVSDDETHVD 684
QEEL+ + LQA + GR+VLR HDH+R+V ER L EQIVSCH HAVVSDDE HV
Sbjct: 515 QEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSCHPRHAVVSDDEVHVH 336
Query: 685 L 687
L
Sbjct: 335 L 333
Score = 50.6 bits (104), Expect(2) = 4e-033
Identities = 22/34 (64%), Positives = 25/34 (73%)
Frame = +1 / -3
Query: 799 IHRGHLVRRALEQHLDELPVDDAVVHRQDMEPRL 900
I G+L RR LE+ LDELPV DAV+H QDME L
Sbjct: 248 IXSGNLERRCLEEPLDELPVKDAVIHGQDMERSL 147
Score = 39.1 bits (79), Expect(2) = 2e-021
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = +2 / -1
Query: 794 PESTVVTW*DEXXXXXXXXXXXXXXXXTARTWNRDSASSTVMAGERR 934
PES VVTW ++ TA+TWN SA + GE R
Sbjct: 253 PESXVVTWNEDVLRSLSMSFLSRTLSSTAKTWNGASAIAATAIGEDR 113
>gb|BJ453869.1|BJ453869 BJ453869 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak44m13 5', mRNA sequence
Length = 641
Score = 142 bits (305), Expect(2) = 7e-052
Identities = 55/77 (71%), Positives = 69/77 (89%)
Frame = -2 / +2
Query: 687 EIDVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFF 508
E++V+LIITDYCMPGMTGYDLL ++K SSS DIPVVIMSSEN+P+RI+RCL++GA EFF
Sbjct: 179 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 358
Query: 507 LKPVKPADMKKLKSHLL 457
LKPV+ +DM KLK H++
Sbjct: 359 LKPVRLSDMSKLKPHIM 409
Score = 111 bits (237), Expect(2) = 3e-034
Identities = 50/73 (68%), Positives = 55/73 (75%)
Frame = +2 / -3
Query: 470 LSFFMSAGFTGFRKNSSAPSCKHLLIRAGTFSDDMITTGMSVSDEDPFTRVSRSYPVMPG 649
LS MS TGFRKNS APS +HLLI G FSDDMITTGMS+ D+D +SRSYPV+PG
Sbjct: 396 LSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSYPVIPG 217
Query: 650 MQ*SVMMRLTSIS 688
MQ*SVMMR T S
Sbjct: 216 MQ*SVMMRFTFTS 178
Score = 82.2 bits (173), Expect(2) = 7e-052
Identities = 35/47 (74%), Positives = 40/47 (85%)
Frame = -2 / +2
Query: 891 FHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGLRDNGA 751
FHVLAVDDS++DRKLIE LLK+SS+QVTTVDSG KAL+ LGL A
Sbjct: 2 FHVLAVDDSVLDRKLIERLLKTSSFQVTTVDSGTKALQFLGLHAEDA 142
Score = 54.2 bits (112), Expect(2) = 3e-034
Identities = 22/31 (70%), Positives = 25/31 (80%)
Frame = +1 / -2
Query: 799 IHRGHLVRRALEQHLDELPVDDAVVHRQDME 891
IH G+L RR LE+ LDELPV DAV+H QDME
Sbjct: 94 IHSGNLERRCLEEPLDELPVKDAVIHGQDME 2
>gb|AL504719.1|AL504719 AL504719 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW06C24V 5', mRNA sequence
Length = 699
Score = 127 bits (271), Expect(2) = 2e-049
Identities = 49/65 (75%), Positives = 60/65 (92%)
Frame = -2 / +2
Query: 687 EIDVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFF 508
E++V+LIITDYCMPGMTGYDLL ++K SSS DIPVVIMSSEN+P+RI+RCL++GA EFF
Sbjct: 326 EVNVNLIITDYCMPGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFF 505
Query: 507 LKPVK 493
LKPV+
Sbjct: 506 LKPVR 520
Score = 105 bits (225), Expect(2) = 9e-033
Identities = 46/64 (71%), Positives = 51/64 (79%)
Frame = +2 / -1
Query: 497 TGFRKNSSAPSCKHLLIRAGTFSDDMITTGMSVSDEDPFTRVSRSYPVMPGMQ*SVMMRL 676
TGFRKNS APS +HLLI G FSDDMITTGMS+ D+D +SRSYPV+PGMQ*SVMMR
Sbjct: 516 TGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSYPVIPGMQ*SVMMRF 337
Query: 677 TSIS 688
T S
Sbjct: 336 TFTS 325
Score = 89.5 bits (189), Expect(2) = 2e-049
Identities = 40/61 (65%), Positives = 46/61 (75%)
Frame = -2 / +2
Query: 933 LRSPAMTVLDAESRFHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGLRDNG 754
L SP AE+ FHVLAVDDS++DRKLIE LLK+SS+QVTTVDSG KAL+ LGL
Sbjct: 107 LSSPMAVAAIAEAPFHVLAVDDSVLDRKLIERLLKTSSFQVTTVDSGTKALQFLGLHAED 286
Query: 753 A 751
A
Sbjct: 287 A 289
Score = 83.5 bits (176), Expect(2) = 4e-022
Identities = 38/61 (62%), Positives = 44/61 (72%)
Frame = +1 / -3
Query: 505 QEELLRAILQAPADPGRHVLRRHDHHRNVRER*GPLHSREQIVSCHAGHAVVSDDETHVD 684
QEEL+ + LQA + GR+VLR HDH+R+V ER L EQIVSCH HAVVSDDE HV
Sbjct: 508 QEELICSFLQASVNSGRYVLR*HDHNRDVPERRRLLDLLEQIVSCHPRHAVVSDDEVHVH 329
Query: 685 L 687
L
Sbjct: 328 L 326
Score = 54.7 bits (113), Expect(2) = 9e-033
Identities = 23/34 (67%), Positives = 26/34 (76%)
Frame = +1 / -3
Query: 799 IHRGHLVRRALEQHLDELPVDDAVVHRQDMEPRL 900
IH G+L RR LE+ LDELPV DAV+H QDME L
Sbjct: 241 IHSGNLERRCLEEPLDELPVKDAVIHGQDMERSL 140
Score = 41.4 bits (84), Expect(2) = 4e-022
Identities = 18/47 (38%), Positives = 22/47 (46%)
Frame = +2 / -1
Query: 794 PESTVVTW*DEXXXXXXXXXXXXXXXXTARTWNRDSASSTVMAGERR 934
PESTVVTW ++ TA+TWN SA + GE R
Sbjct: 246 PESTVVTWNEDVLRSLSMSFLSRTLSSTAKTWNGASAIAATAIGEDR 106
>gb|BI778941.2|BI778941 EBro01_SQ002_C23_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ002_C23 5', mRNA sequence
Length = 568
Score = 121 bits (259), Expect(2) = 9e-042
Identities = 50/69 (72%), Positives = 57/69 (82%)
Frame = -2 / +2
Query: 678 VSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFFLKP 499
V LIITDYCMP MTGYDLL RVK S+ L IPVVIMSSEN PARI RCL +GAEEF +KP
Sbjct: 278 VQLIITDYCMPEMTGYDLLKRVKESAELRGIPVVIMSSENSPARIRRCLDEGAEEFLIKP 457
Query: 498 VKPADMKKL 472
V+P+D+ +L
Sbjct: 458 VRPSDVSRL 484
Score = 89.0 bits (188), Expect(2) = 1e-022
Identities = 42/65 (64%), Positives = 46/65 (70%)
Frame = +2 / -2
Query: 485 SAGFTGFRKNSSAPSCKHLLIRAGTFSDDMITTGMSVSDEDPFTRVSRSYPVMPGMQ*SV 664
S G TG +NSSAPS +H I AG FSDDMITTGM S D FTR SRS PV+ GMQ*SV
Sbjct: 471 SEGRTGLMRNSSAPSSRHRRILAGEFSDDMITTGMPRSSADSFTRFSRS*PVISGMQ*SV 292
Query: 665 MMRLT 679
M+ T
Sbjct: 291 MISCT 277
Score = 69.3 bits (145), Expect(2) = 9e-042
Identities = 29/41 (70%), Positives = 35/41 (85%)
Frame = -2 / +2
Query: 888 HVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGL 766
HVLAVDDS++DR +I LL+SS Y+VTTVDSG KALE+L L
Sbjct: 143 HVLAVDDSLVDRVVISRLLRSSKYRVTTVDSGKKALEVLSL 265
Score = 37.7 bits (76), Expect(2) = 1e-022
Identities = 18/44 (40%), Positives = 24/44 (54%)
Frame = +1 / -1
Query: 799 IHRGHLVRRALEQHLDELPVDDAVVHRQDMEPRLGVQHRHGWGA 930
+HR H V A E+ D+ VD+AVVH QD+ L + W A
Sbjct: 232 VHRRHSVLGAAEEPGDDDAVDEAVVHGQDVRRLLHRRRHRHWPA 101
>gb|CA012046.1|CA012046 HT04E15r HT Hordeum vulgare subsp. vulgare cDNA clone HT04E15
5-PRIME, mRNA sequence
Length = 533
Score = 101 bits (215), Expect(3) = 9e-038
Identities = 42/55 (76%), Positives = 49/55 (89%)
Frame = -2 / +2
Query: 681 DVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAE 517
DV++IITDY MPGMTGY+LL RVK SS L +IPVVIMSSENVP RI+RCL++GAE
Sbjct: 368 DVNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAE 532
Score = 70.7 bits (148), Expect(3) = 9e-038
Identities = 28/48 (58%), Positives = 38/48 (79%)
Frame = -2 / +2
Query: 909 LDAESRFHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGL 766
+ ++ HVLAVDDS++DR +I +L+SS Y+VTTVDS +ALELLGL
Sbjct: 212 VQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLGL 355
Score = 23.5 bits (45), Expect(3) = 9e-038
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = -3 / +3
Query: 782 WSCWG*GTT 756
WSCWG G++
Sbjct: 339 WSCWGWGSS 365
>gb|BQ766925.1|BQ766925 EBro08_SQ007_B17_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ007_B17 5', mRNA sequence
Length = 554
Score = 90.9 bits (192), Expect(3) = 1e-034
Identities = 38/49 (77%), Positives = 43/49 (87%)
Frame = -2 / +3
Query: 681 DVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRC 535
DV++IITDY MPGMTGY+LL RVK SS L +IPVVIMSSENVP RI+RC
Sbjct: 408 DVNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRC 554
Score = 70.7 bits (148), Expect(3) = 1e-034
Identities = 28/48 (58%), Positives = 38/48 (79%)
Frame = -2 / +3
Query: 909 LDAESRFHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGL 766
+ ++ HVLAVDDS++DR +I +L+SS Y+VTTVDS +ALELLGL
Sbjct: 252 VQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLGL 395
Score = 23.5 bits (45), Expect(3) = 1e-034
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = -3 / +1
Query: 782 WSCWG*GTT 756
WSCWG G++
Sbjct: 379 WSCWGWGSS 405
>gb|AW982401.2|AW982401 HVSMEg0003C19f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003C19f, mRNA sequence
Length = 873
Score = 144 bits (310), Expect = 5e-034
Identities = 61/66 (92%), Positives = 63/66 (95%)
Frame = -2 / +3
Query: 654 CMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFFLKPVKPADMKK 475
CMPGMTGYDLL RVKGSSSL DIPVVIMSSENVPARISRCL+DGAEEFFLKPVK ADMKK
Sbjct: 3 CMPGMTGYDLLRRVKGSSSLKDIPVVIMSSENVPARISRCLEDGAEEFFLKPVKLADMKK 182
Query: 474 LKSHLL 457
LKSHL+
Sbjct: 183 LKSHLV 200
Score = 136 bits (292), Expect = 2e-031
Identities = 57/71 (80%), Positives = 62/71 (87%)
Frame = +2 / -3
Query: 443 GCFRFSRCDLSFFMSAGFTGFRKNSSAPSCKHLLIRAGTFSDDMITTGMSVSDEDPFTRV 622
GCFR +R DLSFFMSA FTG RKNSSAPS KHLLI AGTFS+DMITTGMS S++DP T +
Sbjct: 214 GCFRRTR*DLSFFMSASFTGLRKNSSAPSSKHLLILAGTFSEDMITTGMSFSEDDPLTLL 35
Query: 623 SRSYPVMPGMQ 655
SRSYPVMPGMQ
Sbjct: 34 SRSYPVMPGMQ 2
Score = 102 bits (217), Expect = 4e-021
Identities = 42/56 (75%), Positives = 46/56 (82%)
Frame = +1 / -2
Query: 487 GRLHGLQEELLRAILQAPADPGRHVLRRHDHHRNVRER*GPLHSREQIVSCHAGHA 654
G LHGL+EELLRAILQAPADPGRH+LR HDHHRNV + PL +QIVSCHA HA
Sbjct: 170 GELHGLEEELLRAILQAPADPGRHILRGHDHHRNVLQ*G*PLDPPQQIVSCHARHA 3
>gb|BQ762675.1|BQ762675 EBro02_SQ004_O15 _R root, 3 week, hydroponic grown, low nitrogen,
cv Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA
clone EBro02_SQ004_O15 5', mRNA sequence
Length = 571
Score = 86.8 bits (183), Expect(3) = 2e-033
Identities = 37/48 (77%), Positives = 42/48 (87%)
Frame = -2 / +1
Query: 681 DVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISR 538
DV++IITDY MPGMTGY+LL RVK SS L +IPVVIMSSENVP RI+R
Sbjct: 427 DVNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITR 570
Score = 70.7 bits (148), Expect(3) = 2e-033
Identities = 28/48 (58%), Positives = 38/48 (79%)
Frame = -2 / +1
Query: 909 LDAESRFHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLGL 766
+ ++ HVLAVDDS++DR +I +L+SS Y+VTTVDS +ALELLGL
Sbjct: 271 VQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLGL 414
Score = 23.5 bits (45), Expect(3) = 2e-033
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = -3 / +2
Query: 782 WSCWG*GTT 756
WSCWG G++
Sbjct: 398 WSCWGWGSS 424
>gb|CA008912.1|CA008912 HU12I03r HU Hordeum vulgare subsp. vulgare cDNA clone HU12I03
5-PRIME, mRNA sequence
Length = 488
Score = 124 bits (266), Expect(2) = 2e-032
Identities = 50/70 (71%), Positives = 62/70 (88%)
Frame = -2 / +3
Query: 681 DVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFFLK 502
DV++IITDY MPGMTGY+LL RVK SS L +IPVVIMSSENVP RI+RCL++GAE+F LK
Sbjct: 81 DVNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFLLK 260
Query: 501 PVKPADMKKL 472
PV+P+D+ +L
Sbjct: 261 PVRPSDVSRL 290
Score = 34.5 bits (69), Expect(2) = 2e-032
Identities = 14/18 (77%), Positives = 16/18 (88%)
Frame = -2 / +3
Query: 819 YQVTTVDSGNKALELLGL 766
Y+VTTVDS +ALELLGL
Sbjct: 15 YRVTTVDSATRALELLGL 68
>gb|BJ461404.1|BJ461404 BJ461404 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak44m13 3', mRNA sequence
Length = 651
Score = 132 bits (282), Expect = 4e-030
Identities = 52/72 (72%), Positives = 63/72 (87%)
Frame = -2 / -2
Query: 672 LIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFFLKPVK 493
LIITDYCM GMTGYDLL ++K SSS DIPVVIMSSEN+P+RI+RCL++GA EFFLKPV+
Sbjct: 650 LIITDYCMXGMTGYDLLKKIKESSSFRDIPVVIMSSENIPSRINRCLEEGANEFFLKPVR 471
Query: 492 PADMKKLKSHLL 457
+DM KLK H++
Sbjct: 470 LSDMSKLKPHIM 435
Score = 105 bits (224), Expect = 4e-022
Identities = 47/68 (69%), Positives = 52/68 (76%)
Frame = +2 / +1
Query: 470 LSFFMSAGFTGFRKNSSAPSCKHLLIRAGTFSDDMITTGMSVSDEDPFTRVSRSYPVMPG 649
LS MS TGFRKNS APS +HLLI G FSDDMITTGMS+ D+D +SRSYPV+P
Sbjct: 448 LSLLMSDSRTGFRKNSFAPSSRHLLILEGMFSDDMITTGMSLKDDDSLIFLSRSYPVIPX 627
Query: 650 MQ*SVMMR 673
MQ*SVMMR
Sbjct: 628 MQ*SVMMR 651
>gb|BQ465184.1|BQ465184 HU02O05r HU Hordeum vulgare subsp. vulgare cDNA clone HU02O05
5-PRIME, mRNA sequence
Length = 627
Score = 126 bits (270), Expect = 2e-028
Identities = 51/72 (70%), Positives = 63/72 (87%)
Frame = -2 / +1
Query: 687 EIDVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFF 508
E DV++IITDY MPGMTGY+LL RVK SS L +IPVVIMSSENVP RI+RCL++GAE+F
Sbjct: 7 EADVNMIITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFL 186
Query: 507 LKPVKPADMKKL 472
LKPV+P+D+ +L
Sbjct: 187 LKPVRPSDVSRL 222
>gb|BF064456.3|BF064456 HV_CEb0015C20f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0015C20f, mRNA sequence
Length = 622
Score = 116 bits (249), Expect = 1e-025
Identities = 47/65 (72%), Positives = 57/65 (87%)
Frame = -2 / +3
Query: 666 ITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFFLKPVKPA 487
ITDY MPGMTGY+LL RVK SS L +IPVVIMSSENVP RI+RCL++GAE+F LKPV+P+
Sbjct: 3 ITDYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFLLKPVRPS 182
Query: 486 DMKKL 472
D+ +L
Sbjct: 183 DVSRL 197
>gb|BQ663493.1|BQ663493 HU02O05u HU Hordeum vulgare subsp. vulgare cDNA clone HU02O05
3-PRIME, mRNA sequence
Length = 593
Score = 112 bits (240), Expect = 2e-024
Identities = 45/63 (71%), Positives = 55/63 (87%)
Frame = -2 / -1
Query: 660 DYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEEFFLKPVKPADM 481
DY MPGMTGY+LL RVK SS L +IPVVIMSSENVP RI+RCL++GAE+F LKPV+P+D+
Sbjct: 593 DYWMPGMTGYELLKRVKESSELREIPVVIMSSENVPNRITRCLEEGAEDFLLKPVRPSDV 414
Query: 480 KKL 472
+L
Sbjct: 413 SRL 405
>gb|BG344635.2|BG344635 HVSMEg0009O02f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0009O02f, mRNA sequence
Length = 835
Score = 68.9 bits (144), Expect(3) = 6e-023
Identities = 27/47 (57%), Positives = 37/47 (78%)
Frame = -2 / +1
Query: 909 LDAESRFHVLAVDDSIIDRKLIEMLLKSSSYQVTTVDSGNKALELLG 769
+ ++ HVLAVDDS++DR +I +L+SS Y+VTTVDS +ALELLG
Sbjct: 247 VQVQAELHVLAVDDSVVDRAVIAKILRSSKYRVTTVDSATRALELLG 387
Score = 52.8 bits (109), Expect(3) = 6e-023
Identities = 22/36 (61%), Positives = 28/36 (77%)
Frame = -2 / +1
Query: 681 DVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVI 574
DV++IITDY MPGMTGY+LL R+K + L +IP I
Sbjct: 403 DVNMIITDY*MPGMTGYELLKRLKEWTELREIPWTI 510
Score = 23.5 bits (45), Expect(3) = 6e-023
Identities = 6/9 (66%), Positives = 8/9 (88%)
Frame = -3 / +2
Query: 782 WSCWG*GTT 756
WSCWG G++
Sbjct: 374 WSCWGLGSS 400
>gb|BI951782.1|BI951782 HVSMEm0002M12f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0002M12f, mRNA sequence
Length = 812
Score = 102 bits (217), Expect = 4e-021
Identities = 46/62 (74%), Positives = 48/62 (77%)
Frame = -2 / +3
Query: 693 HQEIDVSLIITDYCMPGMTGYDLLTRVKGSSSLTDIPVVIMSSENVPARISRCLQDGAEE 514
HQE+ V+LIITDYCMPGMTGYDL RVKGSS DIPVVIMS ENVP RISR L D E
Sbjct: 69 HQEVGVNLIITDYCMPGMTGYDLFMRVKGSSWGEDIPVVIMSYENVPDRISRRLVDPPPE 248
Query: 513 FF 508
F
Sbjct: 249 RF 254
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 363,339,226
Number of Sequences: 312970
Number of extensions: 5313184
Number of successful extensions: 65259
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 11662735703
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)