TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2619146.2.1
(636 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, n... 147 2e-039
gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedl... 158 2e-038
gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subs... 133 3e-035
gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA li... 144 5e-035
gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. v... 144 5e-035
gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. v... 141 2e-034
gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. v... 128 8e-034
gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Li... 95 5e-032
gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Le... 126 1e-028
gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA li... 118 3e-026
gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA li... 116 2e-025
gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachi... 110 1e-023
>gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ004_N18 5', mRNA sequence
Length = 540
Score = 147 bits (316), Expect(2) = 2e-039
Identities = 60/91 (65%), Positives = 72/91 (79%)
Frame = -3 / +3
Query: 625 LCCSLKPPAFTLKFANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLA 446
+ C PA F N+A D+LETT+VN+QD+ LEA+LD+EG+KAL A+ +MQQG A
Sbjct: 99 ILCCSLKPAPMFTFGNNAALDMLETTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFA 278
Query: 445 YLPGGVCRSSMGRQASYEQAVAWKVVGDDGA 353
YLPGGVC+SSMGRQASYEQAVAWKVVGDD A
Sbjct: 279 YLPGGVCKSSMGRQASYEQAVAWKVVGDDVA 371
Score = 123 bits (264), Expect(3) = 7e-033
Identities = 53/82 (64%), Positives = 59/82 (71%)
Frame = +2 / -2
Query: 359 VVAHHLPRHCLLVRRLPPHARPAHATGEVRQALLHDRGELRGQGLLALLVEDRLQRQVLD 538
V+ HH PRH LL+RRLPPHAR AHA GEVR+ LLHD GEL Q L L+V+DRLQR VL
Sbjct: 365 VITHHFPRHRLLIRRLPPHARLAHAPGEVRETLLHDLGELGAQRLAPLVVQDRLQRDVLQ 186
Query: 539 VHHRRLQDVEAGAVGELERESR 604
VH RRLQ V+ V E E SR
Sbjct: 185 VHQRRLQHVKGRVVAEGEHRSR 120
Score = 122 bits (260), Expect(2) = 3e-030
Identities = 51/89 (57%), Positives = 58/89 (65%)
Frame = +3 / -3
Query: 354 APSSPTTFHATACSYDACRPMLDLHTPPGRYARPCCMIAGSCADRAFWPXXXXXXXXXXX 533
A SSPTTFHATACSYDACRPMLDLHTPPGRYA+PCCM + A A P
Sbjct: 370 ATSSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMS 191
Query: 534 WMFTIVVSRMSKPALLANLSVKAGGFREQ 620
FT VVS MS+ ALL +++ AG +Q
Sbjct: 190 CRFTSVVSSMSRAALLPKVNIGAGFREQQ 104
Score = 35.0 bits (70), Expect(2) = 2e-039
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -3 / +3
Query: 358 GAPQCLALMLANWTFI* 308
GAP CLA M NWTF+*
Sbjct: 372 GAPHCLAFMFVNWTFL* 422
Score = 30.9 bits (61), Expect(3) = 7e-033
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +3 / -3
Query: 315 KVQLASMSARHCGAPSS 365
KVQL +M AR CGAP++
Sbjct: 415 KVQLTNMKARQCGAPAT 365
Score = 29.5 bits (58), Expect(2) = 3e-030
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +2 / -2
Query: 302 RASDEGPVGEHERQALRGA 358
R S EGPV EHE +A+R A
Sbjct: 428 RRSQEGPVDEHEGKAVRRA 372
Score = 23.5 bits (45), Expect(3) = 7e-033
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = +3 / -3
Query: 606 GFREQQSIAS 635
GFREQQS+AS
Sbjct: 121 GFREQQSMAS 92
>gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001C22f, mRNA sequence
Length = 834
Score = 158 bits (340), Expect = 2e-038
Identities = 63/106 (59%), Positives = 78/106 (73%)
Frame = -3 / -3
Query: 625 LCCSLKPPAFTLKFANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLA 446
+ C A FAN AG D+LETT++ +QD+ LE +LD++G+KAL ++ P IMQQG A
Sbjct: 589 IMCCSLKAAPVFTFANQAGLDMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFA 410
Query: 445 YLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
YLPGGVC SSM R SYEQAVAWKV+GDD AP CLA M NW+F+*
Sbjct: 409 YLPGGVCVSSMRRPVSYEQAVAWKVLGDDDAPHCLAFMFVNWSFV* 272
Score = 138 bits (295), Expect = 4e-032
Identities = 62/96 (64%), Positives = 70/96 (72%)
Frame = +2 / +2
Query: 314 EGPVGEHERQALRGAVVAHHLPRHCLLVRRLPPHARPAHATGEVRQALLHDRGELRGQGL 493
EGPV EHE +A+R VVA HLPRH LLVR PPHAR AHA EVR+ LLHD GEL Q L
Sbjct: 278 EGPVDEHEGEAVRRVVVAQHLPRHRLLVRHRPPHARHAHAAWEVRETLLHDFGELGAQRL 457
Query: 494 LALLVEDRLQRQVLDVHHRRLQDVEAGAVGELERES 601
A++VED LQR VL+ + RRLQ V+AG VGE E S
Sbjct: 458 AAIVVEDLLQRDVLERNQRRLQHVQAGLVGEGEHRS 565
Score = 126 bits (269), Expect = 1e-028
Identities = 53/96 (55%), Positives = 60/96 (62%)
Frame = +3 / +3
Query: 315 KVQLASMSARHCGAPSSPTTFHATACSYDACRPMLDLHTPPGRYARPCCMIAGSCADRAF 494
K QL +M AR CGA SSP+TFHATACSYD R MLD HTPPGRYA+PCCMI G+ A
Sbjct: 279 KDQLTNMKARQCGASSSPSTFHATACSYDTGRLMLDTHTPPGRYAKPCCMILGNSEHSAL 458
Query: 495 WPXXXXXXXXXXXWMFTIVVSRMSKPALLANLSVKA 602
P W VVS MS+PA LA ++ A
Sbjct: 459 RPSSSRIFSSEMSWSAISVVSSMSRPAWLAKVNTGA 566
>gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200065F09F1, mRNA sequence
Length = 360
Score = 133 bits (284), Expect(2) = 3e-035
Identities = 53/70 (75%), Positives = 63/70 (90%)
Frame = -3 / +3
Query: 562 ILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYEQAV 383
+LETT+VN+QD+ LEA+LD+EG+KAL A+ +MQQG AYLPGGVC+SSMGRQASYEQAV
Sbjct: 3 MLETTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAV 182
Query: 382 AWKVVGDDGA 353
AWKVVGDD A
Sbjct: 183 AWKVVGDDVA 212
Score = 116 bits (247), Expect(2) = 5e-029
Identities = 48/67 (71%), Positives = 53/67 (79%)
Frame = +2 / -2
Query: 359 VVAHHLPRHCLLVRRLPPHARPAHATGEVRQALLHDRGELRGQGLLALLVEDRLQRQVLD 538
V+ HHLPRH LL+RRLPPHAR AHA GEVR+ LLHD GEL Q L L+V+DRLQR VL
Sbjct: 206 VITHHLPRHRLLIRRLPPHARLAHAPGEVRETLLHDLGELGAQRLAPLVVQDRLQRDVLQ 27
Query: 539 VHHRRLQ 559
VH RRLQ
Sbjct: 26 VHQRRLQ 6
Score = 108 bits (230), Expect(2) = 3e-026
Identities = 44/70 (62%), Positives = 46/70 (65%)
Frame = +3 / -3
Query: 354 APSSPTTFHATACSYDACRPMLDLHTPPGRYARPCCMIAGSCADRAFWPXXXXXXXXXXX 533
A SSPTTFHATACSYDACRPMLDLHTPPGRYA+PCCM + A A P
Sbjct: 211 ATSSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMS 32
Query: 534 WMFTIVVSRM 563
FT VVS M
Sbjct: 31 CRFTSVVSSM 2
Score = 35.0 bits (70), Expect(2) = 3e-035
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -3 / +3
Query: 358 GAPQCLALMLANWTFI* 308
GAP CLA M NWTF+*
Sbjct: 213 GAPHCLAFMFVNWTFL* 263
Score = 30.9 bits (61), Expect(2) = 5e-029
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +3 / -3
Query: 315 KVQLASMSARHCGAPSS 365
KVQL +M AR CGAP++
Sbjct: 256 KVQLTNMKARQCGAPAT 206
Score = 29.5 bits (58), Expect(2) = 3e-026
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +2 / -2
Query: 302 RASDEGPVGEHERQALRGA 358
R S EGPV EHE +A+R A
Sbjct: 269 RRSQEGPVDEHEGKAVRRA 213
>gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 3', mRNA sequence
Length = 659
Score = 144 bits (308), Expect(2) = 5e-035
Identities = 55/110 (50%), Positives = 73/110 (66%)
Frame = -3 / -2
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGR 407
FAN G ++LET+ V +QDL L+ + DE G+KAL +++P +M+QG YLPGGVC S MGR
Sbjct: 484 FANEMGINMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGR 305
Query: 406 QASYEQAVAWKVVGDDGAPQCLALMLANWTFI*GSQHRSAAPDRMPPFAL 257
S+E A+AWKVVG+D CLA NW+F+* S + P PF L
Sbjct: 304 HVSFENAIAWKVVGEDNNVHCLAFCFVNWSFV*SSSQGNPCPSHAAPFLL 155
Score = 23.5 bits (45), Expect(2) = 5e-035
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3 / -2
Query: 634 DAILCCSLK 608
DAILCCS K
Sbjct: 529 DAILCCSFK 503
>gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
3-PRIME, mRNA sequence
Length = 638
Score = 144 bits (308), Expect(2) = 5e-035
Identities = 55/110 (50%), Positives = 73/110 (66%)
Frame = -3 / -1
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGR 407
FAN G ++LET+ V +QDL L+ + DE G+KAL +++P +M+QG YLPGGVC S MGR
Sbjct: 476 FANEMGINMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGR 297
Query: 406 QASYEQAVAWKVVGDDGAPQCLALMLANWTFI*GSQHRSAAPDRMPPFAL 257
S+E A+AWKVVG+D CLA NW+F+* S + P PF L
Sbjct: 296 HVSFENAIAWKVVGEDNNVHCLAFCFVNWSFV*SSSQGNPCPSHAAPFLL 147
Score = 23.5 bits (45), Expect(2) = 5e-035
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3 / -1
Query: 634 DAILCCSLK 608
DAILCCS K
Sbjct: 521 DAILCCSFK 495
>gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. vulgare cDNA clone HX01K23
5-PRIME, mRNA sequence
Length = 633
Score = 141 bits (303), Expect(2) = 2e-034
Identities = 56/110 (50%), Positives = 72/110 (65%)
Frame = -3 / +1
Query: 586 FANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGR 407
FAN G ++LET+ V QDL L+ + DE G+KAL +++P +M+QG YLPGGVC S MGR
Sbjct: 145 FANEMGINMLETSFVATQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGR 324
Query: 406 QASYEQAVAWKVVGDDGAPQCLALMLANWTFI*GSQHRSAAPDRMPPFAL 257
S+E A+AWKVVG+D CLA L NW+F * S + P PF L
Sbjct: 325 HVSFENAIAWKVVGEDNNVHCLAFCLVNWSFE*SSSQGNPCPSHAAPFLL 474
Score = 23.5 bits (45), Expect(2) = 2e-034
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3 / +1
Query: 634 DAILCCSLK 608
DAILCCS K
Sbjct: 100 DAILCCSFK 126
>gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. vulgare cDNA clone HX01B05
3-PRIME, mRNA sequence
Length = 489
Score = 128 bits (274), Expect(2) = 8e-034
Identities = 51/67 (76%), Positives = 60/67 (89%)
Frame = -3 / -1
Query: 553 TTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYEQAVAWK 374
TT+VN+QD+ LEA+LD+EG+KAL A+ +MQQG AYLPGGVC+SSMGRQASYEQAVAWK
Sbjct: 489 TTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAVAWK 310
Query: 373 VVGDDGA 353
VVGDD A
Sbjct: 309 VVGDDVA 289
Score = 111 bits (238), Expect(2) = 1e-027
Identities = 46/65 (70%), Positives = 51/65 (78%)
Frame = +2 / +1
Query: 359 VVAHHLPRHCLLVRRLPPHARPAHATGEVRQALLHDRGELRGQGLLALLVEDRLQRQVLD 538
V+ HHLPRH LL+RRLPPHAR AHA GEVR+ LLHD GEL Q L L+V+DRLQR VL
Sbjct: 295 VITHHLPRHRLLIRRLPPHARLAHAPGEVRETLLHDLGELGAQRLAPLVVQDRLQRDVLQ 474
Query: 539 VHHRR 553
VH RR
Sbjct: 475 VHQRR 489
Score = 102 bits (218), Expect(2) = 2e-024
Identities = 41/66 (62%), Positives = 43/66 (65%)
Frame = +3 / +2
Query: 354 APSSPTTFHATACSYDACRPMLDLHTPPGRYARPCCMIAGSCADRAFWPXXXXXXXXXXX 533
A SSPTTFHATACSYDACRPMLDLHTPPGRYA+PCCM + A A P
Sbjct: 290 ATSSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMS 469
Query: 534 WMFTIV 551
FT V
Sbjct: 470 CRFTSV 487
Score = 35.0 bits (70), Expect(2) = 8e-034
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = -3 / -1
Query: 358 GAPQCLALMLANWTFI* 308
GAP CLA M NWTF+*
Sbjct: 288 GAPHCLAFMFVNWTFL* 238
Score = 30.9 bits (61), Expect(2) = 1e-027
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +3 / +2
Query: 315 KVQLASMSARHCGAPSS 365
KVQL +M AR CGAP++
Sbjct: 245 KVQLTNMKARQCGAPAT 295
Score = 29.5 bits (58), Expect(2) = 2e-024
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +2 / +1
Query: 302 RASDEGPVGEHERQALRGA 358
R S EGPV EHE +A+R A
Sbjct: 232 RRSQEGPVDEHEGKAVRRA 288
>gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cD.08, mRNA sequence
Length = 1501
Score = 95.5 bits (202), Expect(2) = 5e-032
Identities = 38/72 (52%), Positives = 51/72 (70%)
Frame = -3 / +2
Query: 625 LCCSLKPPAFTLKFANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLA 446
+ C A FAN AG D+LETT++ +QD+ LE +LD++G+KAL ++ P IMQQG A
Sbjct: 431 IMCCSLKAAPVFTFANQAGLDMLETTLIALQDISLEKILDDDGRKALCSEFPXIMQQGFA 610
Query: 445 YLPGGVCRSSMG 410
YLP GVC SS+G
Sbjct: 611 YLPXGVCVSSIG 646
Score = 86.3 bits (182), Expect(2) = 1e-026
Identities = 39/64 (60%), Positives = 46/64 (71%)
Frame = +2 / -1
Query: 410 PHARPAHATGEVRQALLHDRGELRGQGLLALLVEDRLQRQVLDVHHRRLQDVEAGAVGEL 589
P+AR AHA EVR+ LLHD GEL Q L A++VED LQR VL+ + RRLQ V+AG VGE
Sbjct: 646 PYARHAHAAWEVRETLLHDXGELGAQRLAAIIVEDLLQRDVLERNQRRLQHVQAGLVGEG 467
Query: 590 ERES 601
E S
Sbjct: 466 EHRS 455
Score = 72.1 bits (151), Expect(2) = 4e-021
Identities = 30/64 (46%), Positives = 35/64 (54%)
Frame = +3 / -2
Query: 411 PMLDLHTPPGRYARPCCMIAGSCADRAFWPXXXXXXXXXXXWMFTIVVSRMSKPALLANL 590
PMLD HTP GRYA+PCCMI G+ A P W VVS MS+PA LA +
Sbjct: 645 PMLDTHTPXGRYAKPCCMIXGNSEHSALRPSSSRIFSSEMSWSAISVVSSMSRPAWLAKV 466
Query: 591 SVKA 602
+ A
Sbjct: 465 NTGA 454
Score = 62.0 bits (129), Expect(2) = 5e-032
Identities = 23/35 (65%), Positives = 27/35 (77%)
Frame = -3 / +3
Query: 412 GRQASYEQAVAWKVVGDDGAPQCLALMLANWTFI* 308
GR SYEQAVAWKV+ DD AP CLA + NW+F+*
Sbjct: 645 GRPVSYEQAVAWKVLXDDDAPHCLAFHVVNWSFV* 749
Score = 52.8 bits (109), Expect(2) = 1e-026
Identities = 22/33 (66%), Positives = 24/33 (72%)
Frame = +3 / -1
Query: 315 KVQLASMSARHCGAPSSPTTFHATACSYDACRP 413
K QL + AR CGA SS +TFHATACSYD RP
Sbjct: 742 KDQLTT*KARQCGASSSXSTFHATACSYDTGRP 644
Score = 48.7 bits (100), Expect(2) = 4e-021
Identities = 20/33 (60%), Positives = 24/33 (72%)
Frame = +2 / -3
Query: 314 EGPVGEHERQALRGAVVAHHLPRHCLLVRRLPP 412
EGPV + + +A+R VVA HLPRH LLVR PP
Sbjct: 743 EGPVDDMKGEAVRRVVVAQHLPRHRLLVRHRPP 645
>gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 523
Score = 126 bits (270), Expect = 1e-028
Identities = 48/96 (50%), Positives = 63/96 (65%)
Frame = -3 / +3
Query: 544 VNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYEQAVAWKVVG 365
V +QDL L+ + DE G+KAL +++P +M+QG YLPGGVC S MGR S+E A+AWKVVG
Sbjct: 6 VALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVG 185
Query: 364 DDGAPQCLALMLANWTFI*GSQHRSAAPDRMPPFAL 257
+D CLA NW+F+* S + P PF L
Sbjct: 186 EDNNVHCLAFCFVNWSFV*SSSQGNPCPSHAAPFLL 293
>gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 3', mRNA sequence
Length = 420
Score = 118 bits (252), Expect = 3e-026
Identities = 44/89 (49%), Positives = 58/89 (65%)
Frame = -3 / -3
Query: 523 LEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQC 344
L+ + DE G+KAL +++P +M+QG YLPGGVC S MGR S+E A+AWKVVG+D C
Sbjct: 418 LDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHC 239
Query: 343 LALMLANWTFI*GSQHRSAAPDRMPPFAL 257
LA NW+F+* S + P PF L
Sbjct: 238 LAFCFVNWSFV*SSSQGNPCPSHAAPFLL 152
>gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 5', mRNA sequence
Length = 447
Score = 116 bits (247), Expect = 2e-025
Identities = 43/86 (50%), Positives = 56/86 (65%)
Frame = -3 / +1
Query: 514 VLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQCLAL 335
+ DE G+KAL +++P +M+QG YLPGGVC S MGR S+E A+AWKVVG+D CLA
Sbjct: 13 IFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHCLAF 192
Query: 334 MLANWTFI*GSQHRSAAPDRMPPFAL 257
NW+F+* S + P PF L
Sbjct: 193 CFVNWSFV*SSSQGNPCPSHAAPFLL 270
>gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009M03f, mRNA sequence
Length = 718
Score = 110 bits (234), Expect = 1e-023
Identities = 44/73 (60%), Positives = 58/73 (79%)
Frame = -3 / +1
Query: 568 FDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYEQ 389
+D+LETT++ +QD+ LE +LD++G+KAL ++ P IMQQG AYLPGGVC SSMGR SYE
Sbjct: 22 WDMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGGVCVSSMGRPVSYEX 201
Query: 388 AVAWKVVGDDGAP 350
AVA KV+ + AP
Sbjct: 202 AVAXKVLAEGEAP 240
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 282,324,494
Number of Sequences: 312970
Number of extensions: 4791049
Number of successful extensions: 80970
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6786673440
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)