TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2618988.2.1
(631 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hyd... 191 1e-057
gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedl... 194 4e-050
gb|AJ462599.1|AJ462599 AJ462599 S00002 Hordeum vulgare subs... 162 5e-040
gb|CA001229.1|CA001229 HS18N07u HS Hordeum vulgare subsp. v... 158 1e-039
gb|BJ450621.1|BJ450621 BJ450621 K. Sato unpublished cDNA li... 155 1e-038
gb|BJ458043.1|BJ458043 BJ458043 K. Sato unpublished cDNA li... 155 1e-038
gb|AV927070.1|AV927070 AV927070 K. Sato unpublished cDNA li... 158 3e-038
gb|AV931900.1|AV931900 AV931900 K. Sato unpublished cDNA li... 158 3e-038
gb|AV929458.1|AV929458 AV929458 K. Sato unpublished cDNA li... 93 5e-036
gb|CA023704.1|CA023704 HZ47C08r HZ Hordeum vulgare subsp. v... 140 7e-033
gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. v... 139 1e-032
gb|BF256192.2|BF256192 HVSMEf0009B02f Hordeum vulgare seedl... 138 2e-032
gb|CA000677.1|CA000677 HS07F12u HS Hordeum vulgare subsp. v... 129 3e-031
emb|AJ496572.1|HVU496572 Hordeum vulgare partial mRNA for p... 119 1e-026
gb|CA015634.1|CA015634 HT14O18r HT Hordeum vulgare subsp. v... 117 6e-026
gb|BF623637.2|BF623637 HVSMEa0003E04f Hordeum vulgare seedl... 114 5e-025
gb|CB881181.1|CB881181 HM09A05w HM Hordeum vulgare subsp. v... 108 8e-025
gb|BM443110.2|BM443110 EBro02_SQ002_J16_R root, 3 week, hyd... 109 2e-023
gb|CA028926.1|CA028926 HZ63K12r HZ Hordeum vulgare subsp. v... 105 2e-022
gb|CA001112.1|CA001112 HS05G15u HS Hordeum vulgare subsp. v... 105 3e-022
gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedl... 104 4e-022
gb|CB859573.1|CB859573 HI10O06w HI Hordeum vulgare subsp. v... 96 2e-021
gb|CA009114.1|CA009114 HU13D11r HU Hordeum vulgare subsp. v... 101 4e-021
gb|CA001379.1|CA001379 HS18D16u HS Hordeum vulgare subsp. v... 95 1e-020
>gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B12 5', mRNA sequence
Length = 552
Score = 191 bits (411), Expect(2) = 1e-057
Identities = 77/93 (82%), Positives = 85/93 (91%)
Frame = -3 / +3
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVP 450
PEPDLD LLPE FL RTK RGLVV+SWAPQRDVLAH +VG FVTHCGWNSVLE+VMAGVP
Sbjct: 69 PEPDLDDLLPECFLDRTKGRGLVVKSWAPQRDVLAHDAVGCFVTHCGWNSVLESVMAGVP 248
Query: 449 MVAWPLYAEQRLNRVFLEKEMQLAVAVEGYDSD 351
M+AWPLYAEQR+N VFLEKEM+LAVA+EGYD +
Sbjct: 249 MLAWPLYAEQRMNAVFLEKEMELAVAMEGYDRE 347
Score = 136 bits (291), Expect = 1e-031
Identities = 61/107 (57%), Positives = 69/107 (64%)
Frame = +1 / -3
Query: 262 SVRFRSILPPSDSKSHRXXXXXXXXXXXXXXXXYPSTATASCISFSRNTRLSRCSA*SGH 441
+V+ RS S+S YPS ATAS ISFSRNT RCSA +GH
Sbjct: 436 TVKVRSRSTLPPSESIIHLTFLATSSASTISLSYPSMATASSISFSRNTAFIRCSAYNGH 257
Query: 442 ATIGTPAITASRTEFQPQCVTKPPTDACASTSRCGAHDLTTRPLSLV 582
A++GTPA+T S TEF PQCVTK PT +CASTSRCGAHD TTRPL LV
Sbjct: 256 ASMGTPAMTDSSTEFHPQCVTKQPTASCASTSRCGAHDFTTRPLPLV 116
Score = 105 bits (224), Expect = 2e-022
Identities = 46/76 (60%), Positives = 58/76 (76%)
Frame = -3 / +3
Query: 392 EMQLAVAVEGYDSDEGIVAAEEVAAKVRWLLESDGGRMLRKRTLAAMRQAKDALREGGES 213
E ++ +AV D +V AEEVA KVRW+++SDGGR+LR+RTL MR+A++AL EGGES
Sbjct: 300 EKEMELAVAMEGYDREMVEAEEVAKKVRWMMDSDGGRVLRERTLTVMRRAEEALLEGGES 479
Query: 212 EATLTGLVDEWKRI*R 165
EATL GLVD W R *R
Sbjct: 480 EATLAGLVDAWIRA*R 527
Score = 92.7 bits (196), Expect(2) = 3e-030
Identities = 45/91 (49%), Positives = 51/91 (56%)
Frame = +3 / -2
Query: 357 VVPFXXXXXXXXXXXXXPVEPLLRVEXXXXXXXXXXXXLQDRVPAAVRHEAAHGRVRQHV 536
V+PF V PLLRV+ L+ RVP+AVRHE AH VRQHV
Sbjct: 341 VIPFHGNGQLHLFLQKHRVHPLLRVQRPRQHGHARHDRLEHRVPSAVRHETAHRVVRQHV 162
Query: 537 PLRRPRPDHETPILGPGQEPLREERVKVRLR 629
LRRPR E P LGP QE LREE ++VRLR
Sbjct: 161 ALRRPRLHDEAPALGPVQETLREEVIQVRLR 69
Score = 58.8 bits (122), Expect(2) = 3e-030
Identities = 26/45 (57%), Positives = 31/45 (68%)
Frame = +1 / -3
Query: 175 RFHSSTNPVSVASDSPPSRSASLACRMAASVRFRSILPPSDSKSH 309
R H+ST+P +VASDSPPS AS A + VR RS LPPS+S H
Sbjct: 517 RIHASTSPANVASDSPPSSRASSARLITVKVRSRSTLPPSESIIH 383
Score = 51.9 bits (107), Expect(2) = 1e-057
Identities = 25/64 (39%), Positives = 31/64 (48%)
Frame = -1 / +1
Query: 340 WRPRRLLPRCDGSWSPMAGGCSGSARWRPCGRPRTRCVRAVSRRPH*PDWWTSGNAFDAV 161
WRPRRLL R G W+ AGGCS S R G + R S+R WW G+ + +
Sbjct: 352 WRPRRLLRRSGG*WTRTAGGCSESGL*R**GGRKRLYSRVGSQRRRWRGWWMHGSVLNVL 531
Query: 160 RGDW 149
W
Sbjct: 532 I*PW 543
>gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017B13f, mRNA sequence
Length = 875
Score = 194 bits (418), Expect(2) = 4e-050
Identities = 77/90 (85%), Positives = 85/90 (94%)
Frame = -3 / +3
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVP 450
PEPDLDALLP GFLART++RGLVV++WAPQRDVLAH +VGGFVTHCGWNSVLEAVMAGVP
Sbjct: 174 PEPDLDALLPPGFLARTQERGLVVKTWAPQRDVLAHGAVGGFVTHCGWNSVLEAVMAGVP 353
Query: 449 MVAWPLYAEQRLNRVFLEKEMQLAVAVEGY 360
M+AWPLYAEQRLNRVFLEKE+ LA AV+ Y
Sbjct: 354 MLAWPLYAEQRLNRVFLEKELGLAAAVDRY 443
Score = 135 bits (289), Expect = 2e-031
Identities = 59/74 (79%), Positives = 60/74 (81%)
Frame = +1 / -2
Query: 361 YPSTATASCISFSRNTRLSRCSA*SGHATIGTPAITASRTEFQPQCVTKPPTDACASTSR 540
Y STA AS SFSRNTRLSRCSA SGHA+IGTPAITAS TEF PQCVT PPT CASTSR
Sbjct: 442 YLSTAAASPSSFSRNTRLSRCSAYSGHASIGTPAITASSTEFHPQCVTNPPTAPCASTSR 263
Query: 541 CGAHDLTTRPLSLV 582
CGAH TT PLS V
Sbjct: 262 CGAHVFTTSPLSWV 221
Score = 23.5 bits (45), Expect(2) = 4e-050
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = -3 / +1
Query: 329 EVAAKVRWLLESDGGRML 276
+V VRW +ESD G L
Sbjct: 475 QVEIDVRWFMESDRGDRL 528
>gb|AJ462599.1|AJ462599 AJ462599 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200036E05F1, mRNA sequence
Length = 360
Score = 162 bits (348), Expect(2) = 5e-040
Identities = 60/93 (64%), Positives = 75/93 (80%)
Frame = -3 / +1
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVP 450
PEPDLDAL+PEGFL RTKDRGLVV+SWAPQ DVL H + G F+THCGWNS LE + AG+P
Sbjct: 28 PEPDLDALMPEGFLERTKDRGLVVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLP 207
Query: 449 MVAWPLYAEQRLNRVFLEKEMQLAVAVEGYDSD 351
++ WPLYAEQR+N+V + M+L V + GY+ +
Sbjct: 208 LLCWPLYAEQRVNKVHIVDGMKLGVEMRGYNEE 306
Score = 21.7 bits (41), Expect(2) = 5e-040
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = -1 / +2
Query: 337 RPRRLLPRCDGSW 299
RPRR R DG W
Sbjct: 314 RPRRWKRRSDGLW 352
>gb|CA001229.1|CA001229 HS18N07u HS Hordeum vulgare subsp. vulgare cDNA clone HS18N07
3-PRIME, mRNA sequence
Length = 544
Score = 158 bits (339), Expect(2) = 1e-039
Identities = 58/93 (62%), Positives = 75/93 (80%)
Frame = -3 / -2
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVP 450
PEPDLDAL+PEGFL RTK RGLVV+SWAPQ +VL H + G F+THCGWNS LE + AG+P
Sbjct: 498 PEPDLDALMPEGFLERTKGRGLVVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLP 319
Query: 449 MVAWPLYAEQRLNRVFLEKEMQLAVAVEGYDSD 351
++ WPLYAEQR+N+V + + M+L V + GY+ +
Sbjct: 318 LLCWPLYAEQRVNKVHIVEGMKLGVEMRGYNEE 220
Score = 24.9 bits (48), Expect(2) = 1e-039
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1 / -3
Query: 316 RCDGSWSPMAGGCSGS 269
R DGSW P A SGS
Sbjct: 191 RSDGSWRPRAAMHSGS 144
>gb|BJ450621.1|BJ450621 BJ450621 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30g17 5', mRNA sequence
Length = 616
Score = 155 bits (332), Expect(2) = 1e-038
Identities = 57/92 (61%), Positives = 74/92 (80%)
Frame = -3 / +1
Query: 626 EPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPM 447
EPDLDAL+PEGFL RTK RGLVV+SWAPQ +VL H + G F+THCGWNS LE + AG+P+
Sbjct: 25 EPDLDALMPEGFLERTKGRGLVVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPL 204
Query: 446 VAWPLYAEQRLNRVFLEKEMQLAVAVEGYDSD 351
+ WPLYAEQR+N+V + + M+L V + GY+ +
Sbjct: 205 LCWPLYAEQRVNKVHIVEGMKLGVEMRGYNEE 300
Score = 24.9 bits (48), Expect(2) = 1e-038
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1 / +2
Query: 316 RCDGSWSPMAGGCSGS 269
R DGSW P A SGS
Sbjct: 329 RSDGSWRPRAAMHSGS 376
>gb|BJ458043.1|BJ458043 BJ458043 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30g17 3', mRNA sequence
Length = 551
Score = 155 bits (332), Expect(2) = 1e-038
Identities = 57/92 (61%), Positives = 74/92 (80%)
Frame = -3 / -3
Query: 626 EPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPM 447
EPDLDAL+PEGFL RTK RGLVV+SWAPQ +VL H + G F+THCGWNS LE + AG+P+
Sbjct: 549 EPDLDALMPEGFLERTKGRGLVVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPL 370
Query: 446 VAWPLYAEQRLNRVFLEKEMQLAVAVEGYDSD 351
+ WPLYAEQR+N+V + + M+L V + GY+ +
Sbjct: 369 LCWPLYAEQRVNKVHIVEGMKLGVEMRGYNEE 274
Score = 24.9 bits (48), Expect(2) = 1e-038
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1 / -1
Query: 316 RCDGSWSPMAGGCSGS 269
R DGSW P A SGS
Sbjct: 245 RSDGSWRPRAAMHSGS 198
>gb|AV927070.1|AV927070 AV927070 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26k01 5', mRNA sequence
Length = 676
Score = 158 bits (339), Expect = 3e-038
Identities = 63/90 (70%), Positives = 75/90 (83%)
Frame = -3 / +3
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVP 450
PEPDLDALLP+GFL RTK RGLVV+SWAPQ DVL H + G FVTHCGWNSVLEAV AGVP
Sbjct: 72 PEPDLDALLPQGFLDRTKGRGLVVKSWAPQVDVLRHRATGAFVTHCGWNSVLEAVAAGVP 251
Query: 449 MVAWPLYAEQRLNRVFLEKEMQLAVAVEGY 360
M+ PL AEQ++N+V + ++M +AV +EGY
Sbjct: 252 MLCLPLEAEQKMNKVCMTEDMGVAVELEGY 341
Score = 100 bits (213), Expect(2) = 5e-024
Identities = 45/74 (60%), Positives = 51/74 (68%)
Frame = +1 / -1
Query: 361 YPSTATASCISFSRNTRLSRCSA*SGHATIGTPAITASRTEFQPQCVTKPPTDACASTSR 540
YPS++TA+ +S +T CSA SG +IGTPA TAS TEF PQCVT P STS
Sbjct: 340 YPSSSTATPMSSVMHTLFIFCSASSGRHSIGTPAATASSTEFHPQCVTNAPVARWRSTST 161
Query: 541 CGAHDLTTRPLSLV 582
CGAHDLTTRPL LV
Sbjct: 160 CGAHDLTTRPLPLV 119
Score = 29.9 bits (59), Expect(2) = 5e-024
Identities = 16/40 (40%), Positives = 17/40 (42%)
Frame = +3 / -3
Query: 213 RLTALTQRVLGLPHGRQRALPEHPPAIGLQEPSHLGSNLL 332
R QR LGL PE PP L HLG +LL
Sbjct: 482 RRAVFLQRRLGLFPASSHTGPELPPVPALDHQPHLGLHLL 363
>gb|AV931900.1|AV931900 AV931900 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26k01 3', mRNA sequence
Length = 631
Score = 158 bits (339), Expect = 3e-038
Identities = 63/90 (70%), Positives = 75/90 (83%)
Frame = -3 / -1
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVP 450
PEPDLDALLP+GFL RTK RGLVV+SWAPQ DVL H + G FVTHCGWNSVLEAV AGVP
Sbjct: 571 PEPDLDALLPQGFLDRTKGRGLVVKSWAPQVDVLRHRATGAFVTHCGWNSVLEAVAAGVP 392
Query: 449 MVAWPLYAEQRLNRVFLEKEMQLAVAVEGY 360
M+ PL AEQ++N+V + ++M +AV +EGY
Sbjct: 391 MLCLPLEAEQKMNKVCMTEDMGVAVELEGY 302
Score = 100 bits (213), Expect(2) = 5e-024
Identities = 45/74 (60%), Positives = 51/74 (68%)
Frame = +1 / +3
Query: 361 YPSTATASCISFSRNTRLSRCSA*SGHATIGTPAITASRTEFQPQCVTKPPTDACASTSR 540
YPS++TA+ +S +T CSA SG +IGTPA TAS TEF PQCVT P STS
Sbjct: 303 YPSSSTATPMSSVMHTLFIFCSASSGRHSIGTPAATASSTEFHPQCVTNAPVARWRSTST 482
Query: 541 CGAHDLTTRPLSLV 582
CGAHDLTTRPL LV
Sbjct: 483 CGAHDLTTRPLPLV 524
Score = 29.9 bits (59), Expect(2) = 5e-024
Identities = 16/40 (40%), Positives = 17/40 (42%)
Frame = +3 / +2
Query: 213 RLTALTQRVLGLPHGRQRALPEHPPAIGLQEPSHLGSNLL 332
R QR LGL PE PP L HLG +LL
Sbjct: 161 RRAVFLQRRLGLFPASSHTGPELPPVPALDHQPHLGLHLL 280
>gb|AV929458.1|AV929458 AV929458 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd14e21 3', mRNA sequence
Length = 683
Score = 93.2 bits (197), Expect(3) = 5e-036
Identities = 35/47 (74%), Positives = 40/47 (85%)
Frame = -3 / -2
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCG 489
PEPDLDAL+PEGFL RTK RGLVV+SWAPQ +VL H + G F+THCG
Sbjct: 676 PEPDLDALMPEGFLERTKGRGLVVKSWAPQVEVLRHRATGAFMTHCG 536
Score = 70.7 bits (148), Expect(3) = 5e-036
Identities = 24/47 (51%), Positives = 36/47 (76%)
Frame = -3 / -3
Query: 491 GWNSVLEAVMAGVPMVAWPLYAEQRLNRVFLEKEMQLAVAVEGYDSD 351
GWNS LE + AG+P++ WPLYAEQR+N+V + + M+L V + GY+ +
Sbjct: 537 GWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVEGMKLGVEMRGYNEE 397
Score = 62.5 bits (130), Expect(3) = 7e-022
Identities = 29/46 (63%), Positives = 31/46 (67%)
Frame = +3 / +2
Query: 492 AVRHEAAHGRVRQHVPLRRPRPDHETPILGPGQEPLREERVKVRLR 629
AVRHE A V Q + LRRPR DHE LGP QEP R ERV+V LR
Sbjct: 539 AVRHECAGRPVAQDLHLRRPRLDHEPSALGPLQEPFRHERVEVGLR 676
Score = 40.9 bits (83), Expect(3) = 7e-022
Identities = 18/32 (56%), Positives = 20/32 (62%)
Frame = +1 / +2
Query: 214 DSPPSRSASLACRMAASVRFRSILPPSDSKSH 309
D PPS SAS A AA R S LPPSD+ +H
Sbjct: 266 DEPPSLSASAAATFAAVTRSLSALPPSDAMTH 361
Score = 37.3 bits (75), Expect(3) = 7e-022
Identities = 18/43 (41%), Positives = 23/43 (53%)
Frame = +1 / +2
Query: 364 PSTATASCISFSRNTRLSRCSA*SGHATIGTPAITASRTEFQP 492
P +T S I + T + CSA SG + G PA+ S EFQP
Sbjct: 410 PLISTPSFIPSTMCTLFTLCSAYSGQHSSGNPAVMPSSVEFQP 538
Score = 24.9 bits (48), Expect(3) = 5e-036
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1 / -1
Query: 316 RCDGSWSPMAGGCSGS 269
R DGSW P A SGS
Sbjct: 368 RSDGSWRPRAAMHSGS 321
>gb|CA023704.1|CA023704 HZ47C08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ47C08
5-PRIME, mRNA sequence
Length = 355
Score = 140 bits (300), Expect = 7e-033
Identities = 62/110 (56%), Positives = 77/110 (70%)
Frame = -3 / +2
Query: 626 EPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPM 447
+ DL LLPEGFL RTK++G+V + APQ+++LAHASVGGFVTH GWNS LE++ GVPM
Sbjct: 26 DADLGELLPEGFLERTKEKGMVWPTKAPQKEILAHASVGGFVTHGGWNSTLESLWFGVPM 205
Query: 446 VAWPLYAEQRLNRVFLEKEMQLAVAVEGYDSDEGIVAAEEVAAKVRWLLE 297
V WPLYAEQ LN L M +AVA+E VAA E+ V+ L+E
Sbjct: 206 VPWPLYAEQHLNAFTLVAYMGVAVAMEVDRKRNNFVAASELERAVKALME 355
>gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. vulgare cDNA clone HI10O06
5-PRIME, mRNA sequence
Length = 635
Score = 139 bits (299), Expect = 1e-032
Identities = 51/69 (73%), Positives = 59/69 (85%)
Frame = -3 / +3
Query: 629 PEPDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVP 450
PEPDLDAL+PEGFL RTKDRGLVV+SWAPQ DVL H + G F+THCGWNS LE + AG+P
Sbjct: 429 PEPDLDALMPEGFLERTKDRGLVVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLP 608
Query: 449 MVAWPLYAE 423
++ WPLYAE
Sbjct: 609 LLCWPLYAE 635
>gb|BF256192.2|BF256192 HVSMEf0009B02f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0009B02f, mRNA sequence
Length = 782
Score = 138 bits (297), Expect = 2e-032
Identities = 54/62 (87%), Positives = 58/62 (93%)
Frame = -3 / +2
Query: 590 LARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLN 411
L RTKDRGLVV SWAPQR+VLAH SVGGFVTHCGWNSVLEAVMAGVPM+ WPLYAEQR+N
Sbjct: 2 LDRTKDRGLVVMSWAPQREVLAHGSVGGFVTHCGWNSVLEAVMAGVPMLGWPLYAEQRMN 181
Query: 410 RV 405
+V
Sbjct: 182 KV 187
Score = 107 bits (228), Expect = 6e-023
Identities = 45/60 (75%), Positives = 48/60 (80%)
Frame = +1 / -3
Query: 403 NTRLSRCSA*SGHATIGTPAITASRTEFQPQCVTKPPTDACASTSRCGAHDLTTRPLSLV 582
NT RCSA SGH +IGTPA+TAS TEF PQ VT PPTD ASTSRCGAHD+TT PLSLV
Sbjct: 189 NTLFIRCSAYSGHPSIGTPAMTASSTEFHPQWVTNPPTDPWASTSRCGAHDMTTSPLSLV 10
>gb|CA000677.1|CA000677 HS07F12u HS Hordeum vulgare subsp. vulgare cDNA clone HS07F12
3-PRIME, mRNA sequence
Length = 556
Score = 129 bits (276), Expect(2) = 3e-031
Identities = 47/78 (60%), Positives = 61/78 (78%)
Frame = -3 / -2
Query: 584 RTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLNRV 405
RTKDRGLVV+SWAPQ DVL H + G F+THCGWNS LE + AG+P++ WPLYAEQR+N+V
Sbjct: 552 RTKDRGLVVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKV 373
Query: 404 FLEKEMQLAVAVEGYDSD 351
+ M+L V + GY+ +
Sbjct: 372 HIVDGMKLGVEMRGYNEE 319
Score = 25.4 bits (49), Expect(2) = 3e-031
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = -1 / -3
Query: 337 RPRRLLPRCDGSWSPMAGGCSGS 269
RPRR R DG W A SGS
Sbjct: 311 RPRRWKRRSDGLWRLRAARHSGS 243
>emb|AJ496572.1|HVU496572 Hordeum vulgare partial mRNA for putative glycosyltransferase (sf2
gene)
Length = 863
Score = 119 bits (255), Expect = 1e-026
Identities = 47/88 (53%), Positives = 63/88 (71%)
Frame = -3 / +1
Query: 575 DRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLNRVFLE 396
DRG +VR WAPQ +L+HA++GGFVTHCGWNSVLEAV AGVPMV WP YA+Q N +
Sbjct: 538 DRGFMVRGWAPQMLILSHAALGGFVTHCGWNSVLEAVSAGVPMVTWPRYADQFNNEKLVV 717
Query: 395 KEMQLAVAVEGYDSDEGIVAAEEVAAKV 312
+ +++ V++ D G+ A E +A +V
Sbjct: 718 ELLKVGVSIGAKDYASGVEAHEVIAGEV 801
>gb|CA015634.1|CA015634 HT14O18r HT Hordeum vulgare subsp. vulgare cDNA clone HT14O18
5-PRIME, mRNA sequence
Length = 622
Score = 117 bits (250), Expect = 6e-026
Identities = 46/88 (52%), Positives = 62/88 (70%)
Frame = -3 / +2
Query: 575 DRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLNRVFLE 396
DRG +VR WAPQ +L+HA++GGFVTHCGWNSVLEAV AGVPMV WP YA+Q N +
Sbjct: 272 DRGFMVRGWAPQMLILSHAALGGFVTHCGWNSVLEAVSAGVPMVTWPRYADQFNNEKLVV 451
Query: 395 KEMQLAVAVEGYDSDEGIVAAEEVAAKV 312
+ +++ ++ D G+ A E +A +V
Sbjct: 452 ELLKVGFSIGAKDYASGVEAHEVIAGEV 535
>gb|BF623637.2|BF623637 HVSMEa0003E04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0003E04f, mRNA sequence
Length = 485
Score = 114 bits (243), Expect = 5e-025
Identities = 39/71 (54%), Positives = 55/71 (77%)
Frame = -3 / +1
Query: 623 PDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMV 444
P+++ L +GF AR KDRG+++R WAPQ +L H ++GGFVTHCGWNS +E + AGVPM+
Sbjct: 250 PEVEEWLADGFEARVKDRGMILRGWAPQVMILWHQAIGGFVTHCGWNSTIEGICAGVPMI 429
Query: 443 AWPLYAEQRLN 411
WP ++EQ +N
Sbjct: 430 TWPHFSEQFVN 462
>gb|CB881181.1|CB881181 HM09A05w HM Hordeum vulgare subsp. vulgare cDNA clone HM09A05
3-PRIME, mRNA sequence
Length = 620
Score = 108 bits (231), Expect(2) = 8e-025
Identities = 41/65 (63%), Positives = 50/65 (76%)
Frame = -3 / -2
Query: 605 LPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYA 426
+PEG+ R DRG++VR WAPQ VLAH SVG F+THCGWNSVLEA AG+P++ WPL
Sbjct: 604 VPEGWEQRVADRGMLVRGWAPQLAVLAHPSVGAFLTHCGWNSVLEAASAGLPVLTWPLVF 425
Query: 425 EQRLN 411
EQ +N
Sbjct: 424 EQFIN 410
Score = 24.4 bits (47), Expect(2) = 8e-025
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -1 / -3
Query: 352 TRASWRPRRLLPRCDGSWSPMAGG 281
TR + RR + R GSW+ +GG
Sbjct: 333 TRTPCQRRRSVRRWPGSWTAESGG 262
>gb|BM443110.2|BM443110 EBro02_SQ002_J16_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ002_J16 5', mRNA sequence
Length = 427
Score = 109 bits (232), Expect = 2e-023
Identities = 37/63 (58%), Positives = 50/63 (79%)
Frame = -3 / +3
Query: 623 PDLDALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMV 444
P+++ L +GF AR KDRG+++RSWAPQ +L H ++GGFVTHCGWNS +E + AGVPM+
Sbjct: 237 PEVEEWLADGFEARVKDRGMILRSWAPQVMILWHQAIGGFVTHCGWNSTIEGICAGVPMI 416
Query: 443 AWP 435
WP
Sbjct: 417 TWP 425
>gb|CA028926.1|CA028926 HZ63K12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ63K12
5-PRIME, mRNA sequence
Length = 384
Score = 105 bits (224), Expect = 2e-022
Identities = 40/119 (33%), Positives = 70/119 (58%)
Frame = -3 / +1
Query: 614 DALLPEGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWP 435
+A L F RT+ RGLV+ WAPQ ++LAH + FV+HCGWNS+LE + G P++AWP
Sbjct: 7 EAKLMSDFTKRTEGRGLVITGWAPQLEILAHGATAFFVSHCGWNSLLEGLSHGKPILAWP 186
Query: 434 LYAEQRLNRVFLEKEMQLAVAVEGYDSDEGIVAAEEVAAKVRWLLESDGGRMLRKRTLA 258
++++Q N ++ ++ + + ++ + A+E+ + ++SD G +R A
Sbjct: 187 MHSDQPWNAGYVCGHLKAGIVMRPWEKSRETLPAKEIQEVINRAMDSDQGIAVRSAAKA 363
>gb|CA001112.1|CA001112 HS05G15u HS Hordeum vulgare subsp. vulgare cDNA clone HS05G15
3-PRIME, mRNA sequence
Length = 560
Score = 105 bits (223), Expect = 3e-022
Identities = 41/80 (51%), Positives = 56/80 (70%)
Frame = -3 / -3
Query: 551 WAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLNRVFLEKEMQLAVA 372
WAPQ +L+HA++GGFVTHCGWNSVLEAV AGVPMV WP YA+Q N + + +++ +
Sbjct: 555 WAPQMLILSHAALGGFVTHCGWNSVLEAVSAGVPMVTWPRYADQFNNEKLVVELLKVGFS 376
Query: 371 VEGYDSDEGIVAAEEVAAKV 312
+ D G+ A E +A +V
Sbjct: 375 IGAKDYASGVEAHEVIAGEV 316
>gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001A18f, mRNA sequence
Length = 612
Score = 104 bits (222), Expect = 4e-022
Identities = 39/75 (52%), Positives = 53/75 (70%)
Frame = -3 / +3
Query: 599 EGFLARTKDRGLVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQ 420
EGF R KDRGL+VR WAPQ +L+H +VGGF+THCGWN+ LEA+ GVP + WP +A+Q
Sbjct: 207 EGFEERVKDRGLLVRGWAPQVTILSHPAVGGFLTHCGWNATLEAISHGVPALTWPNFADQ 386
Query: 419 RLNRVFLEKEMQLAV 375
+ L +++ V
Sbjct: 387 FCSERLLVDVLRVGV 431
>gb|CB859573.1|CB859573 HI10O06w HI Hordeum vulgare subsp. vulgare cDNA clone HI10O06
3-PRIME, mRNA sequence
Length = 511
Score = 96.4 bits (204), Expect(2) = 2e-021
Identities = 34/64 (53%), Positives = 47/64 (73%)
Frame = -3 / -2
Query: 542 QRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLNRVFLEKEMQLAVAVEG 363
Q DVL H + G F+THCGWNS LE + AG+P++ WPLYAEQR+N+V + M+L V + G
Sbjct: 510 QVDVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVDGMKLGVEMRG 331
Query: 362 YDSD 351
Y+ +
Sbjct: 330 YNEE 319
Score = 25.4 bits (49), Expect(2) = 2e-021
Identities = 12/23 (52%), Positives = 12/23 (52%)
Frame = -1 / -3
Query: 337 RPRRLLPRCDGSWSPMAGGCSGS 269
RPRR R DG W A SGS
Sbjct: 311 RPRRWKRRSDGLWRLRAARHSGS 243
>gb|CA009114.1|CA009114 HU13D11r HU Hordeum vulgare subsp. vulgare cDNA clone HU13D11
5-PRIME, mRNA sequence
Length = 581
Score = 101 bits (215), Expect = 4e-021
Identities = 39/66 (59%), Positives = 49/66 (74%)
Frame = -3 / +2
Query: 566 LVVRSWAPQRDVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLNRVFLEKEM 387
LVVR WAPQ +LAH +VGGF+THCGW S LE+V AGVPMV WPL+AEQ +N + +
Sbjct: 272 LVVRGWAPQVAILAHPAVGGFMTHCGWGSTLESVAAGVPMVTWPLFAEQFVNEKLIVDVL 451
Query: 386 QLAVAV 369
+ V+V
Sbjct: 452 GVGVSV 469
>gb|CA001379.1|CA001379 HS18D16u HS Hordeum vulgare subsp. vulgare cDNA clone HS18D16
3-PRIME, mRNA sequence
Length = 473
Score = 94.5 bits (200), Expect(2) = 1e-020
Identities = 32/62 (51%), Positives = 47/62 (75%)
Frame = -3 / -3
Query: 536 DVLAHASVGGFVTHCGWNSVLEAVMAGVPMVAWPLYAEQRLNRVFLEKEMQLAVAVEGYD 357
+VL H + G F+THCGWNS LE + AG+P++ WPLYAEQR+N+V + + M+L V + GY+
Sbjct: 468 EVLRHRATGAFMTHCGWNSTLEGITAGLPLLCWPLYAEQRVNKVHIVEGMKLGVEMRGYN 289
Query: 356 SD 351
+
Sbjct: 288 EE 283
Score = 24.9 bits (48), Expect(2) = 1e-020
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -1 / -1
Query: 316 RCDGSWSPMAGGCSGS 269
R DGSW P A SGS
Sbjct: 254 RSDGSWRPRAAMHSGS 207
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 258,206,920
Number of Sequences: 312970
Number of extensions: 4515961
Number of successful extensions: 95441
Number of sequences better than 1.0e-020: 28
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6701840022
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)