TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594433.2.1
(766 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ468452.1|BJ468452 BJ468452 K. Sato unpublished cDNA li... 255 3e-075
gb|AV915784.1|AV915784 AV915784 K. Sato unpublished cDNA li... 209 4e-069
gb|AV920899.1|AV920899 AV920899 K. Sato unpublished cDNA li... 243 1e-063
gb|AV922058.1|AV922058 AV922058 K. Sato unpublished cDNA li... 243 1e-063
gb|AV921048.1|AV921048 AV921048 K. Sato unpublished cDNA li... 192 4e-053
gb|AV916763.1|AV916763 AV916763 K. Sato unpublished cDNA li... 157 2e-046
gb|CK125269.1|CK125269 BES1824107i19 BES1824 Hordeum vulgar... 155 6e-041
gb|AV922079.1|AV922079 AV922079 K. Sato unpublished cDNA li... 161 3e-039
gb|AJ462078.1|AJ462078 AJ462078 S00002 Hordeum vulgare subs... 111 3e-024
>gb|BJ468452.1|BJ468452 BJ468452 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags38n24 3', mRNA sequence
Length = 739
Score = 255 bits (551), Expect(2) = 3e-075
Identities = 100/129 (77%), Positives = 116/129 (89%)
Frame = -1 / -2
Query: 688 KKVTETFGSGYWCEGCQKNDSECSLRYIMVVKVSDPTGEAWVSVFNEHAEKIIGCSADEL 509
+KVTE GSGYWCEGCQKN ECSLRYIMV+KVSDPTGEAW+S+FN+ AE+I+GCSAD L
Sbjct: 645 RKVTEALGSGYWCEGCQKNYEECSLRYIMVIKVSDPTGEAWLSLFNDQAERIVGCSADNL 466
Query: 508 DRIRKEEGDDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAESKYL 329
DRIRKEEGDD + LKLKEATWVPHLFRVSV Q+EY EKRQRITVRSE+PV+ AAE++Y
Sbjct: 465 DRIRKEEGDDMFQLKLKEATWVPHLFRVSVAQNEYMGEKRQRITVRSESPVDFAAEARYQ 286
Query: 328 LEQIAKLTA 302
LE+IA+LT+
Sbjct: 285 LEEIARLTS 259
Score = 178 bits (383), Expect(2) = 2e-050
Identities = 83/133 (62%), Positives = 89/133 (66%)
Frame = +3 / +2
Query: 294 Y*QAVSFAICSSRYLDSAACSTGASLLTVILCLFSLLYSCCVTLTRNR*GTQVASLSFRA 473
+* VS AI SS YL SAA STG SLLTVILCLFS +YS C TLTRNR GTQVAS S
Sbjct: 251 F*LEVSLAISSSWYLASAAKSTGDSLLTVILCLFSPMYSFCATLTRNRWGTQVASFSLSW 430
Query: 474 XXXXXXXXXXXXXXXXXQPMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSH 653
QP I SA SL ++ ASPVGSETL TMMYL+EHS FFWHPSH
Sbjct: 431 NMSSPSSFLIRSRLSALQPTILSAWSLKRDSQASPVGSETLITMMYLNEHSS*FFWHPSH 610
Query: 654 QYPDPKVSVTFLL 692
QYPDP SVTFL+
Sbjct: 611 QYPDPSASVTFLV 649
Score = 132 bits (283), Expect(2) = 2e-034
Identities = 63/131 (48%), Positives = 81/131 (61%)
Frame = +2 / +1
Query: 293 LLASSKLRYLFKQVLGFSCVLDRRFTPHSDSLPFLVIVFMLCDADAEQVRNPGGFLELQS 472
LLA + L K V FS ++RR PH D LP L V +L +AD EQV NPGGFL+L+
Sbjct: 250 LLAGGEPGDLLKLVPRFSSKVNRRLAPHGDPLPLLAHVLVLRNADTEQVGNPGGFLQLEL 429
Query: 473 ITVVPLLFPDPIKLVGAAANDLLCMLVEHRDPCLASRIGDLDDHDVSQ*AFRVILLASLA 652
V+ LLF DPIK++ AA++ LC+++E P L I DLD HDV Q A ++LLA
Sbjct: 430 EHVIALLFSDPIKIICTAADNPLCLVIEKGQPGLTCWIRDLDHHDVPQRALLIVLLAPFT 609
Query: 653 PVSRSKSFSHL 685
PVS S+ HL
Sbjct: 610 PVS*SECLGHL 642
Score = 106 bits (227), Expect(2) = 4e-026
Identities = 62/131 (47%), Positives = 82/131 (62%)
Frame = -3 / -1
Query: 689 QEGD*NFWIWILVRGMPKE*LGMLTEIHHGRQGLRSYWRGMGLCVQRACREDHWLQRRRA 510
++GD I ILV +P+E G+L E+HHG QGL S G+ + Q RED LQ R +
Sbjct: 646 KKGDRGTRIRILV*RVPEEL*GVLVEVHHGDQGL*SNR*GLAVPFQ*PSREDCRLQCR*S 467
Query: 509 *SDQERGGGRQLCSEAQGSHLGSSPVPRQRHTA*IQ*REKAENHCEE*SAGRARS*IQVP 330
*SDQ+RGG + ++A+GSHLGS PVP QR + RE+AE+H EE AG * +VP
Sbjct: 466 *SDQKRGGR*HVPAQAEGSHLGSPPVPCQRCAERVHGREEAEDHREERVAG*LCC*SEVP 287
Query: 329 A*TDSEAYCLL 297
A* D +A+ L
Sbjct: 286 A*GDRQAHLQL 254
Score = 46.9 bits (96), Expect(2) = 3e-075
Identities = 16/24 (66%), Positives = 17/24 (70%)
Frame = -1 / -1
Query: 754 CMPHKPLKPDQNMWYRACKTCNKK 683
C +KPDQ MWYRAC TCNKK
Sbjct: 712 CANISHIKPDQTMWYRACTTCNKK 641
Score = 40.9 bits (83), Expect(2) = 2e-050
Identities = 16/20 (80%), Positives = 17/20 (85%)
Frame = +3 / +3
Query: 684 FLLQVLQARYHMFWSGLXGL 743
FLLQV+QARYHM WSGL L
Sbjct: 642 FLLQVVQARYHMVWSGLMWL 701
Score = 33.6 bits (67), Expect(2) = 2e-034
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = +1 / +1
Query: 685 SCCRSCKHGTTCSGQ 729
SCCR CK GTT SGQ
Sbjct: 643 SCCRWCKRGTTWSGQ 687
Score = 30.9 bits (61), Expect(2) = 4e-026
Identities = 13/17 (76%), Positives = 14/17 (82%)
Frame = -3 / -3
Query: 734 QA*PEHVVPCLQDLQQE 684
QA*P+HVVP L LQQE
Sbjct: 692 QA*PDHVVPRLHHLQQE 642
>gb|AV915784.1|AV915784 AV915784 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15p23 5', mRNA sequence
Length = 613
Score = 209 bits (451), Expect(2) = 4e-069
Identities = 79/103 (76%), Positives = 88/103 (85%)
Frame = -1 / +3
Query: 757 VCMPHKPLKPDQNMWYRACKTCNKKVTETFGSGYWCEGCQKNDSECSLRYIMVVKVSDPT 578
+C +KPDQ MWYRAC TCNKKVTE GSGYWCEGCQKN ECSLRYIMV+KVSDPT
Sbjct: 195 LCANISHIKPDQTMWYRACTTCNKKVTEALGSGYWCEGCQKNYEECSLRYIMVIKVSDPT 374
Query: 577 GEAWVSVFNEHAEKIIGCSADELDRIRKEEGDDSYALKLKEAT 449
GEAW+S+FN+ AE+I+GCSAD LDRIRKEEGDD + LKLKEAT
Sbjct: 375 GEAWLSLFNDQAERIVGCSADNLDRIRKEEGDDMFQLKLKEAT 503
Score = 137 bits (293), Expect(2) = 6e-045
Identities = 59/99 (59%), Positives = 64/99 (64%)
Frame = +3 / -1
Query: 447 QVASLSFRAXXXXXXXXXXXXXXXXXQPMIFSACSLNTETHASPVGSETLTTMMYLSEHS 626
+VAS S QP I SA SL ++ ASPVGSETL TMMYL+EHS
Sbjct: 505 RVASFSLSWNMSSPSSFLIRSRLSALQPTILSAWSLKRDSQASPVGSETLITMMYLNEHS 326
Query: 627 ESFFWHPSHQYPDPKVSVTFLLQVLQARYHMFWSGLXGL 743
FFWHPSHQYPDP SVTFLLQV+QARYHM WSGL L
Sbjct: 325 S*FFWHPSHQYPDPSASVTFLLQVVQARYHMVWSGLMWL 209
Score = 111 bits (236), Expect(2) = 2e-032
Identities = 51/96 (53%), Positives = 65/96 (67%)
Frame = +2 / -3
Query: 449 GGFLELQSITVVPLLFPDPIKLVGAAANDLLCMLVEHRDPCLASRIGDLDDHDVSQ*AFR 628
GGFL+L+ V+ LLF DPIK++ AA++ LC+++E P L I DLD HDV Q A
Sbjct: 503 GGFLQLELEHVIALLFSDPIKIICTAADNPLCLVIEKGQPGLTCWIRDLDHHDVPQRALL 324
Query: 629 VILLASLAPVSRSKSFSHLLVAGLASTVPHVLVRLE 736
++LLA PVS S+ HLLVAG AS VPH LVRL+
Sbjct: 323 IVLLAPFTPVS*SECLGHLLVAGGASAVPHGLVRLD 216
Score = 94.5 bits (200), Expect(2) = 3e-024
Identities = 51/97 (52%), Positives = 64/97 (65%)
Frame = -3 / +2
Query: 734 QA*PEHVVPCLQDLQQEGD*NFWIWILVRGMPKE*LGMLTEIHHGRQGLRSYWRGMGLCV 555
QA*P+HVVP L LQQEGD I ILV +P+E G+L E+HHG QGL S G+ +
Sbjct: 218 QA*PDHVVPRLHHLQQEGDRGTRIRILV*RVPEEL*GVLVEVHHGDQGL*SNR*GLAVPF 397
Query: 554 QRACREDHWLQRRRA*SDQERGGGRQLCSEAQGSHLG 444
Q RED LQ R +*SDQ+RGG + ++A+GSH G
Sbjct: 398 Q*PSREDCRLQCR*S*SDQKRGGR*HVPAQAEGSHPG 508
Score = 72.5 bits (152), Expect(2) = 4e-069
Identities = 29/40 (72%), Positives = 34/40 (85%)
Frame = -1 / +1
Query: 460 KEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAE 341
++ WVPHLFRVSV Q+EY EKRQRITVRSE+PV+ AAE
Sbjct: 493 RKPPWVPHLFRVSVAQNEYMGEKRQRITVRSESPVDFAAE 612
Score = 63.8 bits (133), Expect(2) = 6e-045
Identities = 29/36 (80%), Positives = 30/36 (83%)
Frame = +3 / -3
Query: 342 SAACSTGASLLTVILCLFSLLYSCCVTLTRNR*GTQ 449
SAA STG SLLTVILCLFS +YS C TLTRNR GTQ
Sbjct: 611 SAAKSTGDSLLTVILCLFSPMYSFCATLTRNRWGTQ 504
Score = 48.3 bits (99), Expect(2) = 2e-032
Identities = 20/37 (54%), Positives = 24/37 (64%)
Frame = +2 / -2
Query: 341 FSCVLDRRFTPHSDSLPFLVIVFMLCDADAEQVRNPG 451
FS ++RR PH D LP L V +L +AD EQV NPG
Sbjct: 612 FSSKVNRRLAPHGDPLPLLAHVLVLRNADTEQVGNPG 502
Score = 37.3 bits (75), Expect(2) = 3e-024
Identities = 17/31 (54%), Positives = 19/31 (61%)
Frame = -2 / +2
Query: 450 PGFLTCSASASHSMNTITRKGRESL*GVKRR 358
PGF TCS SA +T R+GR S *G RR
Sbjct: 503 PGFPTCSVSALRRTSTWARRGRGSP*GASRR 595
>gb|AV920899.1|AV920899 AV920899 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags15p23 3', mRNA sequence
Length = 648
Score = 243 bits (524), Expect = 1e-063
Identities = 95/121 (78%), Positives = 110/121 (90%)
Frame = -1 / -2
Query: 664 SGYWCEGCQKNDSECSLRYIMVVKVSDPTGEAWVSVFNEHAEKIIGCSADELDRIRKEEG 485
SGYWCEGCQKN ECSLRYIMV+KVSDPTGEAW+S+FN+ AE+I+GCSAD LDRIRKEEG
Sbjct: 620 SGYWCEGCQKNYEECSLRYIMVIKVSDPTGEAWLSLFNDQAERIVGCSADNLDRIRKEEG 441
Query: 484 DDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAESKYLLEQIAKLT 305
DD + LKLKEATWVPHLFRVSV Q+EY EKRQRITVRSE+PV+ AAE++Y LE+IA+LT
Sbjct: 440 DDMFQLKLKEATWVPHLFRVSVAQNEYMGEKRQRITVRSESPVDFAAEARYQLEEIARLT 261
Query: 304 A 302
+
Sbjct: 260 S 258
Score = 164 bits (352), Expect = 6e-040
Identities = 77/124 (62%), Positives = 82/124 (66%)
Frame = +3 / +1
Query: 294 Y*QAVSFAICSSRYLDSAACSTGASLLTVILCLFSLLYSCCVTLTRNR*GTQVASLSFRA 473
+* VS AI SS YL SAA STG SLLTVILCLFS +YS C TLTRNR GTQVAS S
Sbjct: 250 F*LEVSLAISSSWYLASAAKSTGDSLLTVILCLFSPMYSFCATLTRNRWGTQVASFSLSW 429
Query: 474 XXXXXXXXXXXXXXXXXQPMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSH 653
QP I SA SL ++ ASPVGSETL TMMYL+EHS FFWHPSH
Sbjct: 430 NMSSPSSFLIRSRLSALQPTILSAWSLKRDSQASPVGSETLITMMYLNEHSS*FFWHPSH 609
Query: 654 QYPD 665
QYPD
Sbjct: 610 QYPD 621
Score = 127 bits (271), Expect = 1e-028
Identities = 60/123 (48%), Positives = 77/123 (62%)
Frame = +2 / +3
Query: 293 LLASSKLRYLFKQVLGFSCVLDRRFTPHSDSLPFLVIVFMLCDADAEQVRNPGGFLELQS 472
LLA + L K V FS ++RR PH D LP L V +L +AD EQV NPGGFL+L+
Sbjct: 249 LLAGGEPGDLLKLVPRFSSKVNRRLAPHGDPLPLLAHVLVLRNADTEQVGNPGGFLQLEL 428
Query: 473 ITVVPLLFPDPIKLVGAAANDLLCMLVEHRDPCLASRIGDLDDHDVSQ*AFRVILLASLA 652
V+ LLF DPIK++ AA++ LC+++E P L I DLD HDV Q A ++LLA
Sbjct: 429 EHVIALLFSDPIKIICTAADNPLCLVIEKGQPGLTCWIRDLDHHDVPQRALLIVLLAPFT 608
Query: 653 PVS 661
PVS
Sbjct: 609 PVS 617
Score = 104 bits (222), Expect = 6e-022
Identities = 60/123 (48%), Positives = 78/123 (63%)
Frame = -3 / -1
Query: 665 IWILVRGMPKE*LGMLTEIHHGRQGLRSYWRGMGLCVQRACREDHWLQRRRA*SDQERGG 486
I ILV +P+E G+L E+HHG QGL S G+ + Q RED LQ R +*SDQ+RGG
Sbjct: 621 IRILV*RVPEEL*GVLVEVHHGDQGL*SNR*GLAVPFQ*PSREDCRLQCR*S*SDQKRGG 442
Query: 485 GRQLCSEAQGSHLGSSPVPRQRHTA*IQ*REKAENHCEE*SAGRARS*IQVPA*TDSEAY 306
+ ++A+GSHLGS PVP QR + RE+AE+H EE AG * +VPA* D +A+
Sbjct: 441 R*HVPAQAEGSHLGSPPVPCQRCAERVHGREEAEDHREERVAG*LCC*SEVPA*GDRQAH 262
Query: 305 CLL 297
L
Sbjct: 261 LQL 253
>gb|AV922058.1|AV922058 AV922058 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j02 3', mRNA sequence
Length = 664
Score = 243 bits (524), Expect = 1e-063
Identities = 95/121 (78%), Positives = 110/121 (90%)
Frame = -1 / -1
Query: 664 SGYWCEGCQKNDSECSLRYIMVVKVSDPTGEAWVSVFNEHAEKIIGCSADELDRIRKEEG 485
SGYWCEGCQKN ECSLRYIMV+KVSDPTGEAW+S+FN+ AE+I+GCSAD LDRIRKEEG
Sbjct: 646 SGYWCEGCQKNYEECSLRYIMVIKVSDPTGEAWLSLFNDQAERIVGCSADNLDRIRKEEG 467
Query: 484 DDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAESKYLLEQIAKLT 305
DD + LKLKEATWVPHLFRVSV Q+EY EKRQRITVRSE+PV+ AAE++Y LE+IA+LT
Sbjct: 466 DDMFQLKLKEATWVPHLFRVSVAQNEYMGEKRQRITVRSESPVDFAAEARYQLEEIARLT 287
Query: 304 A 302
+
Sbjct: 286 S 284
Score = 164 bits (352), Expect = 6e-040
Identities = 77/124 (62%), Positives = 82/124 (66%)
Frame = +3 / +3
Query: 294 Y*QAVSFAICSSRYLDSAACSTGASLLTVILCLFSLLYSCCVTLTRNR*GTQVASLSFRA 473
+* VS AI SS YL SAA STG SLLTVILCLFS +YS C TLTRNR GTQVAS S
Sbjct: 276 F*LEVSLAISSSWYLASAAKSTGDSLLTVILCLFSPMYSFCATLTRNRWGTQVASFSLSW 455
Query: 474 XXXXXXXXXXXXXXXXXQPMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSH 653
QP I SA SL ++ ASPVGSETL TMMYL+EHS FFWHPSH
Sbjct: 456 NMSSPSSFLIRSRLSALQPTILSAWSLKRDSQASPVGSETLITMMYLNEHSS*FFWHPSH 635
Query: 654 QYPD 665
QYPD
Sbjct: 636 QYPD 647
Score = 127 bits (271), Expect = 1e-028
Identities = 60/123 (48%), Positives = 77/123 (62%)
Frame = +2 / +2
Query: 293 LLASSKLRYLFKQVLGFSCVLDRRFTPHSDSLPFLVIVFMLCDADAEQVRNPGGFLELQS 472
LLA + L K V FS ++RR PH D LP L V +L +AD EQV NPGGFL+L+
Sbjct: 275 LLAGGEPGDLLKLVPRFSSKVNRRLAPHGDPLPLLAHVLVLRNADTEQVGNPGGFLQLEL 454
Query: 473 ITVVPLLFPDPIKLVGAAANDLLCMLVEHRDPCLASRIGDLDDHDVSQ*AFRVILLASLA 652
V+ LLF DPIK++ AA++ LC+++E P L I DLD HDV Q A ++LLA
Sbjct: 455 EHVIALLFSDPIKIICTAADNPLCLVIEKGQPGLTCWIRDLDHHDVPQRALLIVLLAPFT 634
Query: 653 PVS 661
PVS
Sbjct: 635 PVS 643
Score = 104 bits (222), Expect = 6e-022
Identities = 60/123 (48%), Positives = 78/123 (63%)
Frame = -3 / -3
Query: 665 IWILVRGMPKE*LGMLTEIHHGRQGLRSYWRGMGLCVQRACREDHWLQRRRA*SDQERGG 486
I ILV +P+E G+L E+HHG QGL S G+ + Q RED LQ R +*SDQ+RGG
Sbjct: 647 IRILV*RVPEEL*GVLVEVHHGDQGL*SNR*GLAVPFQ*PSREDCRLQCR*S*SDQKRGG 468
Query: 485 GRQLCSEAQGSHLGSSPVPRQRHTA*IQ*REKAENHCEE*SAGRARS*IQVPA*TDSEAY 306
+ ++A+GSHLGS PVP QR + RE+AE+H EE AG * +VPA* D +A+
Sbjct: 467 R*HVPAQAEGSHLGSPPVPCQRCAERVHGREEAEDHREERVAG*LCC*SEVPA*GDRQAH 288
Query: 305 CLL 297
L
Sbjct: 287 LQL 279
>gb|AV921048.1|AV921048 AV921048 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16l01 3', mRNA sequence
Length = 651
Score = 192 bits (413), Expect(2) = 4e-053
Identities = 76/101 (75%), Positives = 92/101 (91%)
Frame = -1 / -2
Query: 604 MVVKVSDPTGEAWVSVFNEHAEKIIGCSADELDRIRKEEGDDSYALKLKEATWVPHLFRV 425
+V+KVSDPTGEAW+S+FN+ AE+I+GCSAD LDRIRKEEGDD + LKLKEATWVPHLFRV
Sbjct: 560 LVIKVSDPTGEAWLSLFNDQAERIVGCSADNLDRIRKEEGDDMFQLKLKEATWVPHLFRV 381
Query: 424 SVTQHEYNNEKRQRITVRSEAPVEHAAESKYLLEQIAKLTA 302
SV Q+EY EKRQRITVRSE+PV+ AAE++Y LE+IA+LT+
Sbjct: 380 SVAQNEYMGEKRQRITVRSESPVDFAAEARYQLEEIARLTS 258
Score = 113 bits (242), Expect(2) = 5e-030
Identities = 59/103 (57%), Positives = 63/103 (61%)
Frame = +3 / +1
Query: 294 Y*QAVSFAICSSRYLDSAACSTGASLLTVILCLFSLLYSCCVTLTRNR*GTQVASLSFRA 473
+* VS AI SS YL SAA STG SLLTVILCLFS +YS C TLTRNR GTQVAS S
Sbjct: 250 F*LEVSLAISSSWYLASAAKSTGDSLLTVILCLFSPMYSFCATLTRNRWGTQVASFSLSW 429
Query: 474 XXXXXXXXXXXXXXXXXQPMIFSACSLNTETHASPVGSETLTT 602
QP I SA SL ++ ASPVGSETL T
Sbjct: 430 NMSSPSSFLIRSRLSALQPTILSAWSLKRDSQASPVGSETLIT 558
Score = 103 bits (220), Expect = 1e-021
Identities = 49/102 (48%), Positives = 64/102 (62%)
Frame = +2 / +3
Query: 293 LLASSKLRYLFKQVLGFSCVLDRRFTPHSDSLPFLVIVFMLCDADAEQVRNPGGFLELQS 472
LLA + L K V FS ++RR PH D LP L V +L +AD EQV NPGGFL+L+
Sbjct: 249 LLAGGEPGDLLKLVPRFSSKVNRRLAPHGDPLPLLAHVLVLRNADTEQVGNPGGFLQLEL 428
Query: 473 ITVVPLLFPDPIKLVGAAANDLLCMLVEHRDPCLASRIGDLD 598
V+ LLF DPIK++ AA++ LC+++E P L I DLD
Sbjct: 429 EHVIALLFSDPIKIICTAADNPLCLVIEKGQPGLTCWIRDLD 554
Score = 37.3 bits (75), Expect(2) = 5e-030
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +3 / +3
Query: 633 FFWHPSHQYPD 665
FFWHPSHQYPD
Sbjct: 591 FFWHPSHQYPD 623
Score = 36.3 bits (73), Expect(2) = 4e-053
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = -1 / -3
Query: 664 SGYWCEGCQKN 632
SGYWCEGCQKN
Sbjct: 622 SGYWCEGCQKN 590
>gb|AV916763.1|AV916763 AV916763 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j02 5', mRNA sequence
Length = 573
Score = 157 bits (338), Expect(2) = 2e-046
Identities = 57/77 (74%), Positives = 65/77 (84%)
Frame = -1 / +3
Query: 757 VCMPHKPLKPDQNMWYRACKTCNKKVTETFGSGYWCEGCQKNDSECSLRYIMVVKVSDPT 578
+C +KPDQ MWYRAC TCNKKVTE GSGYWCEGCQKN ECSLRYIMV+KVSDPT
Sbjct: 273 LCANISHIKPDQTMWYRACTTCNKKVTEALGSGYWCEGCQKNYEECSLRYIMVIKVSDPT 452
Query: 577 GEAWVSVFNEHAEKIIG 527
GEAW+S+FN+ AE+I+G
Sbjct: 453 GEAWLSLFNDQAERIVG 503
Score = 134 bits (287), Expect(2) = 4e-033
Identities = 54/73 (73%), Positives = 59/73 (80%)
Frame = +3 / -3
Query: 525 QPMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSHQYPDPKVSVTFLLQVLQ 704
+P I SA SL ++ ASPVGSETL TMMYL+EHS FFWHPSHQYPDP SVTFLLQV+Q
Sbjct: 505 KPTILSAWSLKRDSQASPVGSETLITMMYLNEHSS*FFWHPSHQYPDPSASVTFLLQVVQ 326
Query: 705 ARYHMFWSGLXGL 743
ARYHM WSGL L
Sbjct: 325 ARYHMVWSGLMWL 287
Score = 48.3 bits (99), Expect(2) = 2e-046
Identities = 19/23 (82%), Positives = 20/23 (86%)
Frame = -1 / +1
Query: 526 CSADELDRIRKEEGDDSYALKLK 458
CSAD LDRIRKEEGDD + LKLK
Sbjct: 505 CSADNLDRIRKEEGDDMFQLKLK 573
Score = 27.2 bits (53), Expect(2) = 4e-033
Identities = 11/24 (45%), Positives = 17/24 (70%)
Frame = +2 / -1
Query: 458 LELQSITVVPLLFPDPIKLVGAAA 529
L+L+ V+ LLF DPIK++ A+
Sbjct: 573 LQLELEHVIALLFSDPIKIICTAS 502
>gb|CK125269.1|CK125269 BES1824107i19 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010I197 5-PRIME, mRNA sequence
Length = 917
Score = 155 bits (332), Expect(2) = 6e-041
Identities = 56/77 (72%), Positives = 64/77 (83%)
Frame = -1 / +1
Query: 757 VCMPHKPLKPDQNMWYRACKTCNKKVTETFGSGYWCEGCQKNDSECSLRYIMVVKVSDPT 578
+C +KPDQ MWYRAC TCNKKVTE GSGYWCEGCQKN ECSLRYIMV+KVSDPT
Sbjct: 610 LCANISHIKPDQTMWYRACTTCNKKVTEALGSGYWCEGCQKNYEECSLRYIMVIKVSDPT 789
Query: 577 GEAWVSVFNEHAEKIIG 527
GEAW+S+FN+ A +I+G
Sbjct: 790 GEAWLSLFNDQAXRIVG 840
Score = 132 bits (283), Expect = 2e-030
Identities = 53/73 (72%), Positives = 58/73 (79%)
Frame = +3 / -1
Query: 525 QPMIFSACSLNTETHASPVGSETLTTMMYLSEHSESFFWHPSHQYPDPKVSVTFLLQVLQ 704
+P I A SL ++ ASPVGSETL TMMYL+EHS FFWHPSHQYPDP SVTFLLQV+Q
Sbjct: 842 RPTILXAWSLKRDSQASPVGSETLITMMYLNEHSS*FFWHPSHQYPDPSASVTFLLQVVQ 663
Query: 705 ARYHMFWSGLXGL 743
ARYHM WSGL L
Sbjct: 662 ARYHMVWSGLMWL 624
Score = 32.7 bits (65), Expect(2) = 6e-041
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = -1 / +2
Query: 526 CSADELDRIRKEEGDD 479
CSA+ LDRI KEEGD+
Sbjct: 842 CSANNLDRIXKEEGDE 889
>gb|AV922079.1|AV922079 AV922079 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19j24 3', mRNA sequence
Length = 534
Score = 161 bits (347), Expect = 3e-039
Identities = 65/88 (73%), Positives = 79/88 (89%)
Frame = -1 / -2
Query: 565 VSVFNEHAEKIIGCSADELDRIRKEEGDDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQ 386
+S+FN+ AE+I+GCSAD LDRIRKEEGDD + LKLKEATWVPHLFRVSV Q+EY EKRQ
Sbjct: 533 LSLFNDQAERIVGCSADNLDRIRKEEGDDMFQLKLKEATWVPHLFRVSVAQNEYMGEKRQ 354
Query: 385 RITVRSEAPVEHAAESKYLLEQIAKLTA 302
RITVRSE+PV+ AAE++Y LE+IA+LT+
Sbjct: 353 RITVRSESPVDFAAEARYQLEEIARLTS 270
>gb|AJ462078.1|AJ462078 AJ462078 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200004D02F1, mRNA sequence
Length = 300
Score = 111 bits (238), Expect = 3e-024
Identities = 46/65 (70%), Positives = 56/65 (86%)
Frame = -1 / +2
Query: 496 KEEGDDSYALKLKEATWVPHLFRVSVTQHEYNNEKRQRITVRSEAPVEHAAESKYLLEQI 317
+EEGDD + KLKEATWVPHLFRVSV Q+EY EKRQRITVRSE+PV+ AAE++ LE+I
Sbjct: 20 EEEGDDMFQFKLKEATWVPHLFRVSVAQNEYMGEKRQRITVRSESPVDFAAEARGQLEEI 199
Query: 316 AKLTA 302
A+LT+
Sbjct: 200 ARLTS 214
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 367,527,962
Number of Sequences: 312970
Number of extensions: 5962538
Number of successful extensions: 49730
Number of sequences better than 1.0e-020: 15
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8610591927
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)