TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2594342.2.1
(715 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BI960123.1|BI960123 HVSMEn0023E24f Hordeum vulgare rachi... 168 3e-041
gb|BI960653.1|BI960653 HVSMEn0025K04f Hordeum vulgare rachi... 144 4e-034
gb|CB880728.1|CB880728 HM07J23w HM Hordeum vulgare subsp. v... 119 7e-027
gb|BU994073.1|BU994073 HM05J23r HM Hordeum vulgare subsp. v... 116 5e-026
>gb|BI960123.1|BI960123 HVSMEn0023E24f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0023E24f, mRNA sequence
Length = 640
Score = 168 bits (361), Expect = 3e-041
Identities = 72/102 (70%), Positives = 83/102 (81%)
Frame = -2 / +2
Query: 711 GALCEGLAFNVPLRVRNAGRRSGGPTVFLFSSPPAVPNAPAKPLLGFEKVSLEPGQAGVV 532
G C+ LAF+V L+VRNAG +G +V LFSSPP+ NAPAK LLGFEKVSL PG+AG V
Sbjct: 125 GDHCDDLAFDVKLQVRNAGEVAGAHSVLLFSSPPSAHNAPAKHLLGFEKVSLAPGEAGTV 304
Query: 531 AFKVDVCKDLSVVDELGNRKVALGSHTLHVGDLKHTLNLGV* 406
A +VDVC+DLSVVD+LG RKVALG HTLHVGDLKHT+ L V*
Sbjct: 305 ALRVDVCRDLSVVDQLGGRKVALGGHTLHVGDLKHTVELRV* 430
Score = 95.9 bits (203), Expect(2) = 4e-021
Identities = 43/63 (68%), Positives = 46/63 (73%)
Frame = +1 / -1
Query: 400 RPSDSQVERVLEVPDVQRVAAQRHLPVAQLVHDAEVLAHVHLERHHARLPGLQRHLFEAQ 579
R SDSQ VLEV DVQRVAA+RH AQLVH AEV AHVH +RH A LPG QRHL E +
Sbjct: 436 RSSDSQFHGVLEVADVQRVAAERHFAAAQLVHHAEVPAHVHPQRHRAGLPGRQRHLLEPE 257
Query: 580 QGL 588
Q L
Sbjct: 256 QVL 248
Score = 84.0 bits (177), Expect(2) = 5e-022
Identities = 39/62 (62%), Positives = 42/62 (67%)
Frame = +2 / -2
Query: 527 NATTPACPGSSDTFSKPSRGFAGALGTAGGDENRNTVGPPDLLPAFRTRRGTLNARPSQS 706
NAT PA PG+SDTFS PSR FAGAL GGDEN +T P PAFRT T NAR SQ
Sbjct: 309 NATVPASPGASDTFSNPSRCFAGALWADGGDENSSTECAPATSPAFRTWSLTSNARSSQW 130
Query: 707 AP 712
+P
Sbjct: 129 SP 124
Score = 76.2 bits (160), Expect(3) = 2e-030
Identities = 27/39 (69%), Positives = 29/39 (74%)
Frame = -3 / +3
Query: 572 SKRCRWSPGRRAWWRSRWTCARTSASWTSWATGRWRWAA 456
S+RCRW PGR A WR WTCA TSA WTSWA +WR AA
Sbjct: 264 SRRCRWRPGRPARWRCGWTCAGTSAWWTSWAAAKWRSAA 380
Score = 61.1 bits (127), Expect(3) = 2e-030
Identities = 29/43 (67%), Positives = 31/43 (72%)
Frame = -1 / +1
Query: 709 RALRGPGVQRAPPGAERGQKVRRAHGVPVLVAAGGAQRAGEAP 581
R LR PGV+R PGAER + RRA G VLVAA G QRAGEAP
Sbjct: 127 RPLRRPGVRRQAPGAERRRGGRRALGAAVLVAAVGPQRAGEAP 255
Score = 40.0 bits (81), Expect(2) = 5e-022
Identities = 14/18 (77%), Positives = 15/18 (83%)
Frame = +2 / -2
Query: 404 HQTPKLSVCLRSPTCSVW 457
HQT +VCLRSPTCSVW
Sbjct: 432 HQTRSSTVCLRSPTCSVW 379
Score = 32.7 bits (65), Expect(3) = 2e-030
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -1 / +1
Query: 457 PHAARRGPQAHAQLGSLMAE 398
PHAARR PQAH SLM +
Sbjct: 379 PHAARRRPQAHRGTASLMID 438
Score = 24.9 bits (48), Expect(2) = 4e-021
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1 / -1
Query: 643 AGPSARVPHPEGHVERQALA 702
AG A VPH E VERQ +A
Sbjct: 193 AGHLAGVPHLELDVERQVVA 134
>gb|BI960653.1|BI960653 HVSMEn0025K04f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025K04f, mRNA sequence
Length = 652
Score = 144 bits (309), Expect(2) = 4e-034
Identities = 61/80 (76%), Positives = 67/80 (83%)
Frame = -2 / -2
Query: 645 GGPTVFLFSSPPAVPNAPAKPLLGFEKVSLEPGQAGVVAFKVDVCKDLSVVDELGNRKVA 466
G +V LF SPP+ NAPAK LLGFEKVSL PG+AG VAF+VDVC+DLSVVDELG RKVA
Sbjct: 651 GAHSVLLFXSPPSAHNAPAKHLLGFEKVSLAPGEAGTVAFRVDVCRDLSVVDELGGRKVA 472
Query: 465 LGSHTLHVGDLKHTLNLGV* 406
LG HTLHVGDLKHT+ L V*
Sbjct: 471 LGGHTLHVGDLKHTVELRV* 412
Score = 76.2 bits (160), Expect(4) = 6e-023
Identities = 28/39 (71%), Positives = 29/39 (74%)
Frame = -3 / -3
Query: 572 SKRCRWSPGRRAWWRSRWTCARTSASWTSWATGRWRWAA 456
S+RCRW PGR A WRS WTCA T A WTSWA RWR AA
Sbjct: 578 SRRCRWRPGRPARWRSGWTCAGT*AWWTSWAAARWRSAA 462
Score = 33.6 bits (67), Expect(4) = 6e-023
Identities = 16/21 (76%), Positives = 16/21 (76%)
Frame = -1 / -1
Query: 646 RRAHGVPVLVAAGGAQRAGEA 584
RRA G VLVAA G QRAGEA
Sbjct: 652 RRALGAAVLVAAVGPQRAGEA 590
Score = 32.7 bits (65), Expect(4) = 6e-023
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -1 / -1
Query: 457 PHAARRGPQAHAQLGSLMAE 398
PHAARR PQAH SLM +
Sbjct: 463 PHAARRRPQAHRGTASLMID 404
Score = 22.1 bits (42), Expect(2) = 4e-034
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / -1
Query: 267 KNWIHGQL 244
KNWIHG L
Sbjct: 253 KNWIHGGL 230
Score = 21.7 bits (41), Expect(4) = 6e-023
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = -3 / -2
Query: 269 ERIGSTGSF 243
ERIGSTG F
Sbjct: 255 ERIGSTGGF 229
>gb|CB880728.1|CB880728 HM07J23w HM Hordeum vulgare subsp. vulgare cDNA clone HM07J23
3-PRIME, mRNA sequence
Length = 553
Score = 119 bits (255), Expect(2) = 7e-027
Identities = 50/63 (79%), Positives = 55/63 (87%)
Frame = -2 / +1
Query: 594 PAKPLLGFEKVSLEPGQAGVVAFKVDVCKDLSVVDELGNRKVALGSHTLHVGDLKHTLNL 415
P + LLGFEKVSL PG+AG VAF+VDVC+DLSVVDELG RKVALG HTLHVGDLKHT+ L
Sbjct: 4 PPEALLGFEKVSLAPGEAGTVAFRVDVCRDLSVVDELGGRKVALGGHTLHVGDLKHTVEL 183
Query: 414 GV* 406
V*
Sbjct: 184 RV* 192
Score = 22.1 bits (42), Expect(2) = 7e-027
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / +2
Query: 267 KNWIHGQL 244
KNWIHG L
Sbjct: 350 KNWIHGGL 373
>gb|BU994073.1|BU994073 HM05J23r HM Hordeum vulgare subsp. vulgare cDNA clone HM05J23
5-PRIME, mRNA sequence
Length = 543
Score = 116 bits (249), Expect(2) = 5e-026
Identities = 49/59 (83%), Positives = 53/59 (89%)
Frame = -2 / -3
Query: 582 LLGFEKVSLEPGQAGVVAFKVDVCKDLSVVDELGNRKVALGSHTLHVGDLKHTLNLGV* 406
LLGFEKVSL PG+AG VAF+VDVC+DLSVVDELG RKVALG HTLHVGDLKHT+ L V*
Sbjct: 538 LLGFEKVSLAPGEAGTVAFRVDVCRDLSVVDELGGRKVALGGHTLHVGDLKHTVELRV* 362
Score = 22.1 bits (42), Expect(2) = 5e-026
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / -2
Query: 267 KNWIHGQL 244
KNWIHG L
Sbjct: 203 KNWIHGGL 180
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 333,023,319
Number of Sequences: 312970
Number of extensions: 5953772
Number of successful extensions: 127420
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7889507874
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)