TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2591194.2.1
(591 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ462026.1|AJ462026 AJ462026 S00002 Hordeum vulgare subs... 192 1e-048
gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots... 95 9e-042
gb|AL502321.1|AL502321 AL502321 Hordeum vulgare Barke roots... 151 3e-036
gb|AJ461534.1|AJ461534 AJ461534 S00002 Hordeum vulgare subs... 110 6e-024
>gb|AJ462026.1|AJ462026 AJ462026 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200079D08F1, mRNA sequence
Length = 300
Score = 192 bits (414), Expect = 1e-048
Identities = 76/79 (96%), Positives = 77/79 (97%)
Frame = -1 / +1
Query: 576 IARYTLQVYPQSWTAIYMPLDNVGMWNVRSESWARQYLGQQFYLRVYSPANSWRDENPIP 397
IARYTLQVYP +WTAIYMPLDNVGMWNVRSESW RQYLGQQFYLRVYSPANSWRDENPIP
Sbjct: 1 IARYTLQVYPGAWTAIYMPLDNVGMWNVRSESWGRQYLGQQFYLRVYSPANSWRDENPIP 180
Query: 396 KNALLCGRASGRRTRPL*S 340
KNALLCGRASGRRTRPL*S
Sbjct: 181 KNALLCGRASGRRTRPL*S 237
Score = 170 bits (365), Expect = 7e-042
Identities = 71/78 (91%), Positives = 73/78 (93%)
Frame = +2 / -2
Query: 341 LQRGLVRRPEARPQSSAFLGIGFSSRHEFAGE*TRR*NCWPRYCLAQLSDLTFHIPTLSS 520
LQ GLVRRPEARPQSSAFLG+GFSSRHE AGE*TRR*NCWPRYCL QLSDLTFHIPTLSS
Sbjct: 236 LQSGLVRRPEARPQSSAFLGMGFSSRHELAGE*TRR*NCWPRYCLPQLSDLTFHIPTLSS 57
Query: 521 GM*IAVHD*GYTCNVYRA 574
GM*IAVH GYTC+VYRA
Sbjct: 56 GM*IAVHAPGYTCSVYRA 3
Score = 101 bits (216), Expect = 3e-021
Identities = 49/76 (64%), Positives = 52/76 (68%)
Frame = -3 / +3
Query: 568 VHIAGVPSVMDGYLHAAGQRGDVEREVRELGQXXXXXXXXXXXXXAGELVAGREPDPQER 389
VH AGV +DG LHAAGQRGDVEREV ELGQ AG+LVAGREP PQER
Sbjct: 9 VHAAGVSWSVDGDLHAAGQRGDVEREVGELGQAVPGPAVLPAGLLAGQLVAGREPHPQER 188
Query: 388 AALRPGLWTPDQASLK 341
AALRP L P QA+LK
Sbjct: 189 AALRPRLRPPHQAALK 236
>gb|AL505038.1|AL505038 AL505038 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07D06V 5', mRNA sequence
Length = 694
Score = 95.0 bits (201), Expect(2) = 9e-042
Identities = 38/40 (95%), Positives = 38/40 (95%)
Frame = -1 / +2
Query: 459 QQFYLRVYSPANSWRDENPIPKNALLCGRASGRRTRPL*S 340
QQFYLRVYSPANSWR ENPIPKNALLCGRASGRRTRP *S
Sbjct: 338 QQFYLRVYSPANSWRXENPIPKNALLCGRASGRRTRPF*S 457
Score = 95.0 bits (201), Expect(2) = 9e-042
Identities = 36/40 (90%), Positives = 38/40 (95%)
Frame = -1 / +3
Query: 582 NAIARYTLQVYPQSWTAIYMPLDNVGMWNVRSESWARQYL 463
+AIARYTLQVYP +WTAIYMPLDNVGMWNVRSESW RQYL
Sbjct: 216 DAIARYTLQVYPGAWTAIYMPLDNVGMWNVRSESWGRQYL 335
Score = 88.6 bits (187), Expect(2) = 5e-036
Identities = 36/40 (90%), Positives = 37/40 (92%)
Frame = +2 / -2
Query: 341 LQRGLVRRPEARPQSSAFLGIGFSSRHEFAGE*TRR*NCW 460
LQ GLVRRPEARPQSSAFLG+GFS RHE AGE*TRR*NCW
Sbjct: 456 LQNGLVRRPEARPQSSAFLGMGFSXRHELAGE*TRR*NCW 337
Score = 82.2 bits (173), Expect(2) = 5e-036
Identities = 34/39 (87%), Positives = 36/39 (92%)
Frame = +2 / -1
Query: 464 RYCLAQLSDLTFHIPTLSSGM*IAVHD*GYTCNVYRAMA 580
RYCL QLSDLTFHIPTLSSGM*IAVH GYTC+VYRA+A
Sbjct: 334 RYCLPQLSDLTFHIPTLSSGM*IAVHAPGYTCSVYRAIA 218
>gb|AL502321.1|AL502321 AL502321 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07D06u 3', mRNA sequence
Length = 487
Score = 151 bits (324), Expect = 3e-036
Identities = 59/63 (93%), Positives = 59/63 (93%)
Frame = -1 / -3
Query: 528 YMPLDNVGMWNVRSESWARQYLGQQFYLRVYSPANSWRDENPIPKNALLCGRASGRRTRP 349
Y PLDNVGMWNVRSESW RQYLGQQFYLRVYSPANSWRDENPIPKNALLCGRASGRR RP
Sbjct: 452 YXPLDNVGMWNVRSESWGRQYLGQQFYLRVYSPANSWRDENPIPKNALLCGRASGRRXRP 273
Query: 348 L*S 340
*S
Sbjct: 272 F*S 264
Score = 139 bits (299), Expect = 9e-033
Identities = 58/63 (92%), Positives = 59/63 (93%)
Frame = +2 / +1
Query: 341 LQRGLVRRPEARPQSSAFLGIGFSSRHEFAGE*TRR*NCWPRYCLAQLSDLTFHIPTLSS 520
LQ GL RRPEARPQSSAFLG+GFSSRHE AGE*TRR*NCWPRYCL QLSDLTFHIPTLSS
Sbjct: 265 LQNGLXRRPEARPQSSAFLGMGFSSRHELAGE*TRR*NCWPRYCLPQLSDLTFHIPTLSS 444
Query: 521 GM* 529
GM*
Sbjct: 445 GM* 453
>gb|AJ461534.1|AJ461534 AJ461534 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200012C08F1, mRNA sequence
Length = 300
Score = 110 bits (235), Expect = 6e-024
Identities = 43/73 (58%), Positives = 54/73 (73%)
Frame = -1 / +1
Query: 582 NAIARYTLQVYPQSWTAIYMPLDNVGMWNVRSESWARQYLGQQFYLRVYSPANSWRDENP 403
+A AR+T+QVYP W+AI + LDN GMWN+RS +W R YLGQQ YLRV++ S +E
Sbjct: 55 DAQARHTVQVYPNGWSAILVSLDNQGMWNLRSANWDRHYLGQQLYLRVWTAEQSLSNEYS 234
Query: 402 IPKNALLCGRASG 364
IP NA+LCGRA G
Sbjct: 235 IPTNAILCGRADG 273
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 225,980,100
Number of Sequences: 312970
Number of extensions: 3075158
Number of successful extensions: 42106
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6150422805
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)