TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2576887.2.1
(1138 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG368983.1|BG368983 HVSMEi0021P17f Hordeum vulgare 20 DA... 403 e-113
gb|BE422325.1|BE422325 HWM023cA.11r ITEC HWM Barley Leaf Li... 316 1e-085
gb|AL506966.1|AL506966 AL506966 Hordeum vulgare Barke devel... 316 2e-085
gb|CA024441.1|CA024441 HZ49F05r HZ Hordeum vulgare subsp. v... 195 4e-079
gb|BI960324.1|BI960324 HVSMEn0024D18f Hordeum vulgare rachi... 163 3e-070
gb|BG343833.1|BG343833 HVSMEg0006O01f Hordeum vulgare pre-a... 162 5e-070
gb|BU985887.1|BU985887 HF08O22r HF Hordeum vulgare subsp. v... 251 5e-066
gb|BF627174.2|BF627174 HVSMEb0004C08f Hordeum vulgare seedl... 137 3e-065
gb|BU998770.1|BU998770 HI12B24r HI Hordeum vulgare subsp. v... 134 5e-061
gb|AV925297.1|AV925297 AV925297 K. Sato unpublished cDNA li... 225 5e-058
gb|AV930451.1|AV930451 AV930451 K. Sato unpublished cDNA li... 225 5e-058
gb|AL507460.1|AL507460 AL507460 Hordeum vulgare Barke devel... 211 6e-054
gb|BF629887.2|BF629887 HVSMEb0007D12f Hordeum vulgare seedl... 130 1e-039
gb|AV910880.1|AV910880 AV910880 K. Sato unpublished cDNA li... 156 2e-037
gb|CA027968.1|CA027968 HZ60J12r HZ Hordeum vulgare subsp. v... 120 1e-026
gb|BQ665780.1|BQ665780 HZ01B14u HZ Hordeum vulgare subsp. v... 104 1e-021
>gb|BG368983.1|BG368983 HVSMEi0021P17f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0021P17f, mRNA sequence
Length = 854
Score = 403 bits (875), Expect(2) = e-113
Identities = 151/183 (82%), Positives = 164/183 (89%)
Frame = +1 / +2
Query: 145 KPQREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCR 324
K QREFDYFAL+LQWPGTIC+STRHCCA NGCCR EPL TFTIHGLWPDYDDGTWPSCCR
Sbjct: 155 KEQREFDYFALALQWPGTICSSTRHCCAVNGCCRKEPLHTFTIHGLWPDYDDGTWPSCCR 334
Query: 325 RTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDELQY 504
RT F+MDKI PLK LDKYWPSLYCS S TCFSG+G FWAHEWEKHGTCSAP V++ELQY
Sbjct: 335 RTSFDMDKITPLKPTLDKYWPSLYCSSSSTCFSGRGPFWAHEWEKHGTCSAPAVREELQY 514
Query: 505 FTLALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVCKRGSIEELR 684
F+ ALDLY KYNVTEML++G I VS+G EYALSDVIDTIKHAFGGSPQI+C +GS+ ELR
Sbjct: 515 FSTALDLYFKYNVTEMLATGDILVSHG*EYALSDVIDTIKHAFGGSPQIIC*KGSLHELR 694
Query: 685 LCF 693
LCF
Sbjct: 695 LCF 703
Score = 327 bits (708), Expect = 8e-089
Identities = 145/185 (78%), Positives = 153/185 (82%)
Frame = -3 / -3
Query: 704 NSLSKHNLSSSIEPLLQTICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE* 525
N KHNLSS EP Q ICG+PPKACLMVSMTSLSAYS P+ET I P+ +ISVTLYL+*
Sbjct: 714 NHWVKHNLSS*SEPF*QMICGEPPKACLMVSMTSLSAYS*PWETRISPVANISVTLYLK* 535
Query: 524 RSRARVKYCNSS*TTGAEQVPCFSHS*AQKSPLPLKHVPDLEQ*RDGQYLSSTSFSGNIL 345
RSRA +KYCNSS T GAE VPCFSHS*AQK PLPLK V + EQ*RDGQYLSS F G IL
Sbjct: 534 RSRAVLKYCNSSRTAGAEHVPCFSHS*AQKGPLPLKQVEEDEQ*RDGQYLSSVGFRGVIL 355
Query: 344 SISNWVRRQHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMVPGHWRESAK* 165
S+S VRRQHDGQVPSS*SGHSPWIVNVWSGS RQQPL AQQWRVE QMVPGH SAK*
Sbjct: 354 SMSKLVRRQHDGQVPSS*SGHSPWIVNVWSGSFRQQPLTAQQWRVEEQMVPGHCSASAK* 175
Query: 164 SNSRC 150
SNSRC
Sbjct: 174 SNSRC 160
Score = 178 bits (384), Expect = 4e-044
Identities = 82/148 (55%), Positives = 93/148 (62%)
Frame = +3 / +1
Query: 249 GAAPDVHDPRAMAGLRRRDLAVVLPPHPIRDGQDIATEXXXXXXXXXXXXXXIWNMLQRQ 428
GAAP VHDPRA+AGLR RDLAVVLPPH R GQD A+E ++++ Q
Sbjct: 259 GAAPHVHDPRAVAGLR*RDLAVVLPPHQFRHGQDNASEAHAGQVLAVPLLFVLFHLFQW* 438
Query: 429 GTLLGSRVGETWNLLCPCGSG*ITVFHPCP*PLLQV*RYGNAVKWRDTGFKW*GICTERC 608
G LLGS VGE WN+L PC SG ITV CP* LLQ+*R N WR G ICTE C
Sbjct: 439 GPLLGS*VGEAWNMLGPCCSGRITVLQHCP*SLLQI*RDRNVGDWRYPGLPRLRICTE*C 618
Query: 609 HRYHQTCFWGITTNCLQEGFNRRT*VML 692
H +HQ CFWG+T + L EG RT VML
Sbjct: 619 H*HHQACFWGLTADHLLEGLTSRTKVML 702
Score = 132 bits (283), Expect(2) = 1e-030
Identities = 65/118 (55%), Positives = 81/118 (68%)
Frame = -2 / -2
Query: 693 EA*PKFFD*TPLANNLW*SPKSMFDGIDDIAQCIFLTI*NLYPST*QHFRNVILGVEVKG 514
+A*P F * LA++L *+PKSM DG++DI QCIFL + + S+ QHF +VI VE+KG
Sbjct: 703 KA*P*FVK*ALLADDLR*APKSMLDGVNDITQCIFLAVGDQDISSRQHFCHVIFEVEIKG 524
Query: 513 KGEIL*FILNHRGRAGSMFLPLVSPEESLAAEACSRFGAVEGRPVLVKHLLQWQYLVH 340
E+L*F N RGRA SM LPL+SPE + E R VEGRPVLV+ LQ +YLVH
Sbjct: 523 SAEVL*FFPNSRGRACSMLLPLMSPEGAPTTETSGRGRTVEGRPVLVQRGLQRRYLVH 350
Score = 128 bits (274), Expect(2) = 4e-029
Identities = 65/122 (53%), Positives = 76/122 (62%)
Frame = -1 / -1
Query: 691 SIT*VLRLNPSCKQFVVIPQKHV*WYR*HRSVHIPYHLKPVSLHLTAFP*RYTWSRGQGQ 512
SIT V ++PS + V PQKH *W +*H SVHI +P L F RY SR QGQ
Sbjct: 701 SITLVREVSPSSR*SAVSPQKHA*WCQ*HHSVHILSRGRPGYLQSPTFLSRYI*SRDQGQ 522
Query: 511 G*NTVIHPEPQGQSRFHVSPTREPRRVPCR*SMFQIWSSRGTASTCQAPPSVAISCPSRI 332
*+TVI PE QG S FH SPT EPRR P *+ ++ +SRGTASTC A S A+SCP R
Sbjct: 521 C*STVILPEQQGPSMFHASPTHEPRRGPYH*NKWKRTNSRGTASTCPAWASEALSCPCRN 342
Query: 331 GC 326
C
Sbjct: 341 WC 336
Score = 24.9 bits (48), Expect(2) = e-113
Identities = 10/24 (41%), Positives = 11/24 (45%)
Frame = +2 / +3
Query: 749 YQKASTARGTSPCQRMIPLCLPIQ 820
Y+ S A PC MIP P Q
Sbjct: 762 YRNTSVALEKFPCPHMIPFVSPXQ 833
Score = 24.4 bits (47), Expect(2) = 1e-030
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -1 / -1
Query: 196 CRATGGRARSSRTPAA 149
C AT ARSSRT AA
Sbjct: 206 CPATAAPARSSRTRAA 159
Score = 23.5 bits (45), Expect(2) = 4e-029
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -2 / -2
Query: 189 PLEGEREVVELPLRL 145
PL+ +REVVEL L L
Sbjct: 199 PLQRQREVVELALLL 155
>gb|BE422325.1|BE422325 HWM023cA.11r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM023cA.11, mRNA sequence
Length = 1078
Score = 316 bits (685), Expect = 1e-085
Identities = 122/155 (78%), Positives = 140/155 (90%)
Frame = +1 / +3
Query: 376 KYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDELQYFTLALDLYSKYNVTEML 555
KYWPSLYCS S TCFSG+G FWAHEWEKHGTCSAP V++ELQYF+ ALDLY KYNVTEML
Sbjct: 123 KYWPSLYCSSSSTCFSGRGPFWAHEWEKHGTCSAPAVREELQYFSTALDLYFKYNVTEML 302
Query: 556 SSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVCKRGSIEELRLCFDKELKPRDCLTTSL 735
++G I VSNGKEYALSDVIDTIKHAFGGSPQI+CK+GS++ELRLCF K+LKPRDCLTTS
Sbjct: 303 ATGDILVSNGKEYALSDVIDTIKHAFGGSPQIICKKGSVQELRLCFTKDLKPRDCLTTSA 482
Query: 736 ANGSVSKSKHCPRYITLPTYDPLVLANSTVEIMTQ 840
++SKSKHCPR I+LPTYDP+VLANST+EI+ +
Sbjct: 483 MYKNLSKSKHCPRKISLPTYDPIVLANSTLEIVPE 587
Score = 257 bits (556), Expect = 7e-068
Identities = 114/152 (75%), Positives = 125/152 (82%)
Frame = -3 / -1
Query: 830 ISTVELASTRGSYVGKVMYRGQCLLFDTLPFAKEVVRQSRGFNSLSKHNLSSSIEPLLQT 651
IS+VELAST GSYVG+ ++RGQC FD + EVVRQSRGF SL KHNLSS EP LQ
Sbjct: 577 ISSVELASTMGSYVGREIFRGQCFDFDRFLYIAEVVRQSRGFKSLVKHNLSS*TEPFLQM 398
Query: 650 ICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE*RSRARVKYCNSS*TTGAE 471
ICG+PPKACLMVSMTSLSAYSLP ET I P+ +ISVTLYL+*RSRA +KYCNSS T GAE
Sbjct: 397 ICGEPPKACLMVSMTSLSAYSLPLETRISPVANISVTLYLK*RSRAVLKYCNSSRTAGAE 218
Query: 470 QVPCFSHS*AQKSPLPLKHVPDLEQ*RDGQYL 375
VPCFSHS*AQK PLPLK V + EQ*RDGQYL
Sbjct: 217 HVPCFSHS*AQKGPLPLKQVEEDEQ*RDGQYL 122
Score = 170 bits (365), Expect = 2e-041
Identities = 84/154 (54%), Positives = 104/154 (67%)
Frame = -2 / -3
Query: 831 DLYS*IGKHKGIIRWQGDVPRAVLAF*YTSIRQRSGKAITRLQFFVEA*PKFFD*TPLAN 652
+L *IG++ GII WQGD RA+L F + RS KAITRLQ F EA*P F +* LA+
Sbjct: 578 NLQC*IGEYDGIICWQGDFSRAML*FR*VLVHCRSSKAITRLQIFGEA*P*FLN*ALLAD 399
Query: 651 NLW*SPKSMFDGIDDIAQCIFLTI*NLYPST*QHFRNVILGVEVKGKGEIL*FILNHRGR 472
+L *+PKSM DG++DI QCIF T+ + S+ QHF +VI VE+KG E+L*F N RGR
Sbjct: 398 DLR*APKSMLDGVNDITQCIFFTVGDQDISSRQHFCHVIFEVEIKGSAEVL*FFPNSRGR 219
Query: 471 AGSMFLPLVSPEESLAAEACSRFGAVEGRPVLVK 370
A SM LPL+SPE + E R VEGRPVL +
Sbjct: 218 ACSMLLPLMSPEGAPTTETSGRGRTVEGRPVLAR 117
Score = 160 bits (344), Expect(2) = 9e-039
Identities = 76/142 (53%), Positives = 94/142 (66%)
Frame = +3 / +2
Query: 405 IWNMLQRQGTLLGSRVGETWNLLCPCGSG*ITVFHPCP*PLLQV*RYGNAVKWRDTGFKW 584
++++ Q G LLGS VGE WN+L PC SG ITV CP* LLQ+*R N WR G +
Sbjct: 152 LFHLFQW*GPLLGS*VGEAWNMLGPCCSGRITVLQHCP*SLLQI*RDRNVGDWRYPGLQR 331
Query: 585 *GICTERCHRYHQTCFWGITTNCLQEGFNRRT*VMLRQRIEAS*LPYHFFGEWKCIKKQA 764
* ICTE CH +HQ CFWG+T + LQEG + RT VML QR EAS*LPY+F + I+ +A
Sbjct: 332 *RICTE*CH*HHQACFWGLTADHLQEGLSSRTKVMLHQRFEAS*LPYYFGNVQEPIEIKA 511
Query: 765 LPAVHHLANV*SPCACQFNCRD 830
LP+ + AN+*S QFN D
Sbjct: 512 LPSKNLPANI*SHRTRQFNTGD 577
Score = 155 bits (333), Expect = 4e-037
Identities = 80/149 (53%), Positives = 93/149 (62%)
Frame = -1 / -2
Query: 820 LNWQAQGDHTLAR*CTAGSACFLIHFHSPKKW*GNHEASILCRSIT*VLRLNPSCKQFVV 641
LNW+ + DH LA G+A I + K *GNHEAS L SIT VL L+PSC+ V
Sbjct: 567 LNWRVRWDHMLAGRFFEGNALISIGSCTLPK**GNHEASNLW*SITLVLELSPSCR*SAV 388
Query: 640 IPQKHV*WYR*HRSVHIPYHLKPVSLHLTAFP*RYTWSRGQGQG*NTVIHPEPQGQSRFH 461
PQKH *W +*H SVHI Y +P L F RY SR QGQ *+TVI PE QG S FH
Sbjct: 387 SPQKHA*WCQ*HHSVHILYRWRPGYLQSPTFLSRYI*SRDQGQC*STVILPEQQGPSMFH 208
Query: 460 VSPTREPRRVPCR*SMFQIWSSRGTASTC 374
SPT EPRR P *+ ++ +SRGTASTC
Sbjct: 207 ASPTHEPRRGPYH*NKWKRTNSRGTASTC 121
Score = 129 bits (277), Expect = 2e-029
Identities = 74/164 (45%), Positives = 83/164 (50%)
Frame = +2 / +1
Query: 377 STGRPSTAPNLEHASAARDSSGLTSGRNMEPALPLWFRMNYXXXXXXXXXXXXXXXRKCC 556
STGRPST L S SGL SGR+ME A PL F NY +KC
Sbjct: 124 STGRPSTVRPLPLVSVVGAPSGLMSGRSMEHARPLLFGKNYSTSALPLISTSNIT*QKCW 303
Query: 557 QVEGYRFQMVRNMH*AMSSIPSNMLLGDHHKLFARGVQSKNLGYASTKN*SLVIALPLLW 736
++E V+NMH* MS PS+MLLG H + AR Q KN GYAS K *SLVIAL L
Sbjct: 304 RLEISWSPTVKNMH*VMSLTPSSMLLGAHRRSSARRAQFKN*GYASPKI*SLVIALLLRQ 483
Query: 737 RMEVYQKASTARGTSPCQRMIPLCLPIQL*RS*HSSMNLRCLQL 868
Y+ S A SPCQ MIP PIQ R + C L
Sbjct: 484 CTRTYRNQSIALEKSPCQHMIPSYSPIQHWRLSQKPATMGCTSL 615
Score = 24.0 bits (46), Expect(2) = 9e-039
Identities = 8/23 (34%), Positives = 11/23 (47%)
Frame = +1 / +3
Query: 169 FALSLQWPGTICASTRHCCATNG 237
F S WP C+S+ C + G
Sbjct: 111 FGTSKYWPSLYCSSSSTCFSGRG 179
>gb|AL506966.1|AL506966 AL506966 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04M09T 5',
mRNA sequence
Length = 561
Score = 316 bits (684), Expect = 2e-085
Identities = 114/138 (82%), Positives = 122/138 (88%)
Frame = +1 / +3
Query: 145 KPQREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCR 324
K QREFDYFAL+LQWPGTIC+STRHCCA NGCCR EPL TFTIHGLWPDYDDGTWP CCR
Sbjct: 147 KEQREFDYFALALQWPGTICSSTRHCCAVNGCCRKEPLHTFTIHGLWPDYDDGTWPXCCR 326
Query: 325 RTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDELQY 504
T F+MDKI PLK LDKYWPSLYCS S TCFSG+G FWAHEWEKHGTCSAP V++ELQY
Sbjct: 327 HTSFDMDKITPLKPTLDKYWPSLYCSSSSTCFSGRGPFWAHEWEKHGTCSAPAVREELQY 506
Query: 505 FTLALDLYSKYNVTEMLS 558
F+ ALDLY KYNVTEML+
Sbjct: 507 FSTALDLYFKYNVTEMLA 560
Score = 240 bits (519), Expect = 9e-063
Identities = 106/135 (78%), Positives = 111/135 (82%)
Frame = -3 / -3
Query: 554 SISVTLYLE*RSRARVKYCNSS*TTGAEQVPCFSHS*AQKSPLPLKHVPDLEQ*RDGQYL 375
+ISVTLYL+ RSRA +KYCNSS T GAE VPCFSHS*AQK PLPLK V + EQ*RDGQYL
Sbjct: 556 NISVTLYLKXRSRAVLKYCNSSRTAGAEHVPCFSHS*AQKGPLPLKQVEEDEQ*RDGQYL 377
Query: 374 SSTSFSGNILSISNWVRRQHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMV 195
S F G ILS+S V RQH GQVPSS*SGHSPWIVNVWSGS RQQPL AQQWRVE QMV
Sbjct: 376 SRVGFKGVILSMSKLVWRQHXGQVPSS*SGHSPWIVNVWSGSFRQQPLTAQQWRVEEQMV 197
Query: 194 PGHWRESAK*SNSRC 150
PGH SAK*SNSRC
Sbjct: 196 PGHCSASAK*SNSRC 152
Score = 120 bits (257), Expect = 1e-026
Identities = 57/101 (56%), Positives = 64/101 (63%)
Frame = +3 / +2
Query: 249 GAAPDVHDPRAMAGLRRRDLAVVLPPHPIRDGQDIATEXXXXXXXXXXXXXXIWNMLQRQ 428
GAAP VHDPRA+AGLR RDLAVVLPPH R GQD A E ++++ Q
Sbjct: 251 GAAPHVHDPRAVAGLR*RDLAVVLPPHQFRHGQDNAFEAHSGQVLAVPLLFVLFHLFQW* 430
Query: 429 GTLLGSRVGETWNLLCPCGSG*ITVFHPCP*PLLQV*RYGN 551
G LLGS VGE WN+L PC SG ITV CP* LQ+*R N
Sbjct: 431 GPLLGS*VGEAWNMLGPCCSGRITVLQHCP*SXLQI*RDRN 553
>gb|CA024441.1|CA024441 HZ49F05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ49F05
5-PRIME, mRNA sequence
Length = 592
Score = 195 bits (420), Expect(2) = 4e-079
Identities = 72/133 (54%), Positives = 93/133 (69%)
Frame = +1 / +1
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDE 495
C T F M+KI L+ +L++YWPSLYC + TCF G+G FWAHEW HGTC P +QDE
Sbjct: 193 CRPTTTFNMNKIAMLRPILERYWPSLYCGDTSTCFGGRGPFWAHEWATHGTCGYPEIQDE 372
Query: 496 LQYFTLALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVCKRGSIE 675
YF+ AL LYSKYNVT+ L I NG++YA++ ++ I HAFG P +VCK GS++
Sbjct: 373 YDYFSTALYLYSKYNVTKALRKAHIYPRNGRKYAVAHIVAAIDHAFGRLPHLVCKNGSLQ 552
Query: 676 ELRLCFDKELKPR 714
ELRLCF K+ +PR
Sbjct: 553 ELRLCFHKDYQPR 591
Score = 120 bits (257), Expect(2) = 4e-079
Identities = 39/60 (65%), Positives = 46/60 (76%)
Frame = +1 / +1
Query: 151 QREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCRRT 330
Q FD++ L+LQWPG++C T HCC++NGCCRS PL FTIHGLWP Y G WPSCCR T
Sbjct: 25 QAGFDHYVLALQWPGSVCRQTNHCCSSNGCCRSNPLNWFTIHGLWPQYSYGGWPSCCRPT 204
Score = 95.5 bits (202), Expect(2) = 2e-033
Identities = 61/129 (47%), Positives = 69/129 (53%)
Frame = -3 / -3
Query: 713 RGFNSLSKHNLSSSIEPLLQTICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLY 534
RG+ SL K +LSS EP LQT CG P A M + +AY LPF + S VTLY
Sbjct: 590 RGW*SLWKQSLSSCREPFLQTRCGSRPNAWSMAATMCATAYFLPFRG*MCAFLSAFVTLY 411
Query: 533 LE*RSRARVKYCNSS*TTGAEQVPCFSHS*AQKSPLPLKHVPDLEQ*RDGQYLSSTSFSG 354
L *R A K SS +G VP +HS AQK PLP K V Q* DGQYLS S
Sbjct: 410 LL*R*SAVEK*SYSSCISG*PHVP*VAHSWAQKGPLPPKQVEVSPQ*SDGQYLSRIGLSI 231
Query: 353 NILSISNWV 327
IL + N V
Sbjct: 230 AILFMLNVV 204
Score = 68.0 bits (142), Expect(2) = 2e-033
Identities = 31/57 (54%), Positives = 32/57 (56%)
Frame = -3 / -3
Query: 320 QHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMVPGHWRESAK*SNSRC 150
Q DG P GHSPWIVN G +RQQPL QQW V Q PGH R S SN C
Sbjct: 194 QQDGHPP*LYCGHSPWIVNQLRGFDRQQPLELQQWLVWRQTEPGHCRAST*WSNPAC 24
>gb|BI960324.1|BI960324 HVSMEn0024D18f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0024D18f, mRNA sequence
Length = 647
Score = 163 bits (350), Expect(2) = 3e-070
Identities = 60/114 (52%), Positives = 77/114 (67%)
Frame = +1 / +3
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDE 495
C T F M+KI L+ +L++YWPSLYC + TCF G+G FWAHEW HGTC P +QDE
Sbjct: 303 CRPTTTFNMNKIAMLRPILERYWPSLYCGDTSTCFGGRGPFWAHEWATHGTCGYPEIQDE 482
Query: 496 LQYFTLALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVC 657
YF+ AL LYSKYNVT+ L I NG++YA++ ++ I HAFG P +VC
Sbjct: 483 YDYFSTALYLYSKYNVTKALRKAHIYPRNGRKYAVAHIVAAIDHAFGRLPHLVC 644
Score = 122 bits (262), Expect(2) = 3e-070
Identities = 40/60 (66%), Positives = 46/60 (76%)
Frame = +1 / +3
Query: 151 QREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCRRT 330
Q FDY+ L+LQWPG++C T HCC++NGCCRS PL FTIHGLWP Y G WPSCCR T
Sbjct: 135 QAGFDYYVLALQWPGSVCRQTNHCCSSNGCCRSNPLNWFTIHGLWPQYSYGGWPSCCRPT 314
Score = 77.6 bits (163), Expect(2) = 9e-028
Identities = 51/111 (45%), Positives = 57/111 (51%)
Frame = -3 / -2
Query: 659 LQTICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE*RSRARVKYCNSS*TT 480
LQT CG P A M + +AY LPF + S VTLYL *R A K SS +
Sbjct: 646 LQTRCGSRPNAWSMAATMCATAYFLPFRG*MCAFLSAFVTLYLL*R*SAVEK*SYSSCIS 467
Query: 479 GAEQVPCFSHS*AQKSPLPLKHVPDLEQ*RDGQYLSSTSFSGNILSISNWV 327
G VP +HS AQK PLP K V Q* DGQYLS S IL + N V
Sbjct: 466 G*PHVP*VAHSWAQKGPLPPKQVEVSPQ*SDGQYLSRIGLSIAILFMLNVV 314
Score = 66.6 bits (139), Expect(2) = 9e-028
Identities = 31/57 (54%), Positives = 32/57 (56%)
Frame = -3 / -2
Query: 320 QHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMVPGHWRESAK*SNSRC 150
Q DG P G SPWIVN G +RQQPL QQW V Q PGH R S *SN C
Sbjct: 304 QQDGHPP*LYCGQSPWIVNQLRGFDRQQPLELQQWLVWRQTEPGHCRAST**SNPAC 134
>gb|BG343833.1|BG343833 HVSMEg0006O01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0006O01f, mRNA sequence
Length = 1051
Score = 162 bits (349), Expect(2) = 5e-070
Identities = 59/120 (49%), Positives = 80/120 (66%)
Frame = +1 / +1
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDE 495
C T F M+KI L+ +L++YWPSLYC + TCF G+G FWAHEW HGTC P +QDE
Sbjct: 283 CRPTTTFNMNKIAMLRPILERYWPSLYCGDTSTCFGGRGPFWAHEWATHGTCGYPEIQDE 462
Query: 496 LQYFTLALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVCKRGSIE 675
YF+ AL LYSKYNVT+ L I NG++YA++ ++ I HAF P +VC+ +++
Sbjct: 463 YDYFSTALYLYSKYNVTKALRKAHIYPRNGRKYAVAHIVAXIDHAFXRLPHLVCRTATLQ 642
Score = 122 bits (262), Expect(2) = 5e-070
Identities = 40/60 (66%), Positives = 46/60 (76%)
Frame = +1 / +1
Query: 151 QREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCRRT 330
Q FDY+ L+LQWPG++C T HCC++NGCCRS PL FTIHGLWP Y G WPSCCR T
Sbjct: 115 QAGFDYYVLALQWPGSVCRQTNHCCSSNGCCRSNPLNWFTIHGLWPQYSYGGWPSCCRPT 294
Score = 73.5 bits (154), Expect(2) = 1e-026
Identities = 50/112 (44%), Positives = 57/112 (50%)
Frame = -3 / -3
Query: 662 LLQTICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE*RSRARVKYCNSS*T 483
+LQT CG A M + +AY LPF + S VTLYL *R A K SS
Sbjct: 629 VLQTRCGSRXNAWSMXATMCATAYFLPFRG*MCAFLSAFVTLYLL*R*SAVEK*SYSSCI 450
Query: 482 TGAEQVPCFSHS*AQKSPLPLKHVPDLEQ*RDGQYLSSTSFSGNILSISNWV 327
+G VP +HS AQK PLP K V Q* DGQYLS S IL + N V
Sbjct: 449 SG*PHVP*VAHSWAQKGPLPPKQVEVSPQ*SDGQYLSRIGLSIAILFMLNVV 294
Score = 66.6 bits (139), Expect(2) = 1e-026
Identities = 31/57 (54%), Positives = 32/57 (56%)
Frame = -3 / -3
Query: 320 QHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMVPGHWRESAK*SNSRC 150
Q DG P G SPWIVN G +RQQPL QQW V Q PGH R S *SN C
Sbjct: 284 QQDGHPP*LYCGQSPWIVNQLRGFDRQQPLELQQWLVWRQTEPGHCRAST**SNPAC 114
>gb|BU985887.1|BU985887 HF08O22r HF Hordeum vulgare subsp. vulgare cDNA clone HF08O22
5-PRIME, mRNA sequence
Length = 549
Score = 251 bits (543), Expect = 5e-066
Identities = 100/129 (77%), Positives = 117/129 (90%)
Frame = +1 / +2
Query: 454 EKHGTCSAPVVQDELQYFTLALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAF 633
EKHGTCSAP V++ELQYF+ ALDLY KYNVTEML++G I VSNGKEYALSDVIDTIKHAF
Sbjct: 11 EKHGTCSAPAVREELQYFSTALDLYFKYNVTEMLATGDILVSNGKEYALSDVIDTIKHAF 190
Query: 634 GGSPQIVCKRGSIEELRLCFDKELKPRDCLTTSLANGSVSKSKHCPRYITLPTYDPLVLA 813
GGSPQI+CK+GS++ELRLCF K+LKPRDCLTTS ++SKSKHCPR I+LPTYDP+VLA
Sbjct: 191 GGSPQIICKKGSVQELRLCFTKDLKPRDCLTTSAMYKNLSKSKHCPRKISLPTYDPIVLA 370
Query: 814 NSTVEIMTQ 840
NST+EI+ +
Sbjct: 371 NSTLEIVPE 397
Score = 212 bits (457), Expect = 3e-054
Identities = 93/126 (73%), Positives = 103/126 (81%)
Frame = -3 / -1
Query: 830 ISTVELASTRGSYVGKVMYRGQCLLFDTLPFAKEVVRQSRGFNSLSKHNLSSSIEPLLQT 651
IS+VELAST GSYVG+ ++RGQC FD + EVVRQSRGF SL KHNLSS EP LQ
Sbjct: 387 ISSVELASTMGSYVGREIFRGQCFDFDRFLYIAEVVRQSRGFKSLVKHNLSS*TEPFLQM 208
Query: 650 ICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE*RSRARVKYCNSS*TTGAE 471
ICG+PPKACLMVSMTSLSAYSLP ET I P+ +ISVTLYL+*RSRA +KYCNSS T GAE
Sbjct: 207 ICGEPPKACLMVSMTSLSAYSLPLETRISPVANISVTLYLK*RSRAVLKYCNSSRTAGAE 28
Query: 470 QVPCFS 453
VPCFS
Sbjct: 27 HVPCFS 10
Score = 148 bits (317), Expect = 7e-035
Identities = 69/126 (54%), Positives = 83/126 (65%)
Frame = +3 / +1
Query: 453 GETWNLLCPCGSG*ITVFHPCP*PLLQV*RYGNAVKWRDTGFKW*GICTERCHRYHQTCF 632
GE WN+L PC SG ITV CP* LLQ+*R N WR G + * ICTE CH +HQ CF
Sbjct: 10 GEAWNMLGPCCSGRITVLQHCP*SLLQI*RDRNVGDWRYPGLQR*RICTE*CH*HHQACF 189
Query: 633 WGITTNCLQEGFNRRT*VMLRQRIEAS*LPYHFFGEWKCIKKQALPAVHHLANV*SPCAC 812
WG+T + LQEG + RT VML QR EAS*LPY+F + I+ +ALP+ + AN+*S
Sbjct: 190 WGLTADHLQEGLSSRTKVMLHQRFEAS*LPYYFGNVQEPIEIKALPSKNLPANI*SHRTR 369
Query: 813 QFNCRD 830
QFN D
Sbjct: 370 QFNTGD 387
Score = 143 bits (307), Expect = 2e-033
Identities = 71/127 (55%), Positives = 88/127 (69%)
Frame = -2 / -3
Query: 831 DLYS*IGKHKGIIRWQGDVPRAVLAF*YTSIRQRSGKAITRLQFFVEA*PKFFD*TPLAN 652
+L *IG++ GII WQGD RA+L F + RS KAITRLQ F EA*P F +* LA+
Sbjct: 388 NLQC*IGEYDGIICWQGDFSRAML*FR*VLVHCRSSKAITRLQIFGEA*P*FLN*ALLAD 209
Query: 651 NLW*SPKSMFDGIDDIAQCIFLTI*NLYPST*QHFRNVILGVEVKGKGEIL*FILNHRGR 472
+L *+PKSM DG++DI QCIF T+ + S+ QHF +VI VE+KG E+L*F N RGR
Sbjct: 208 DLR*APKSMLDGVNDITQCIFFTVGDQDISSRQHFCHVIFEVEIKGSAEVL*FFPNSRGR 29
Query: 471 AGSMFLP 451
A SM LP
Sbjct: 28 ACSMLLP 8
Score = 123 bits (264), Expect = 1e-027
Identities = 65/124 (52%), Positives = 75/124 (60%)
Frame = -1 / -2
Query: 820 LNWQAQGDHTLAR*CTAGSACFLIHFHSPKKW*GNHEASILCRSIT*VLRLNPSCKQFVV 641
LNW+ + DH LA G+A I + K *GNHEAS L SIT VL L+PSC+ V
Sbjct: 377 LNWRVRWDHMLAGRFFEGNALISIGSCTLPK**GNHEASNLW*SITLVLELSPSCR*SAV 198
Query: 640 IPQKHV*WYR*HRSVHIPYHLKPVSLHLTAFP*RYTWSRGQGQG*NTVIHPEPQGQSRFH 461
PQKH *W +*H SVHI Y +P L F RY SR QGQ *+TVI PE QG S FH
Sbjct: 197 SPQKHA*WCQ*HHSVHILYRWRPGYLQSPTFLSRYI*SRDQGQC*STVILPEQQGPSMFH 18
Query: 460 VSPT 449
SP+
Sbjct: 17 ASPS 6
Score = 107 bits (229), Expect = 9e-023
Identities = 61/139 (43%), Positives = 70/139 (50%)
Frame = +2 / +3
Query: 452 GRNMEPALPLWFRMNYXXXXXXXXXXXXXXXRKCCQVEGYRFQMVRNMH*AMSSIPSNML 631
GR+ME A PL F NY +KC ++E V+NMH* MS PS+ML
Sbjct: 9 GRSMEHARPLLFGKNYSTSALPLISTSNIT*QKCWRLEISWSPTVKNMH*VMSLTPSSML 188
Query: 632 LGDHHKLFARGVQSKNLGYASTKN*SLVIALPLLWRMEVYQKASTARGTSPCQRMIPLCL 811
LG H + AR Q KN GYAS K *SLVIAL L Y+ S A SPCQ MIP
Sbjct: 189 LGAHRRSSARRAQFKN*GYASPKI*SLVIALLLRQCTRTYRNQSIALEKSPCQHMIPSYS 368
Query: 812 PIQL*RS*HSSMNLRCLQL 868
PIQ R + C L
Sbjct: 369 PIQHWRLSQKPATMGCTSL 425
>gb|BF627174.2|BF627174 HVSMEb0004C08f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0004C08f, mRNA sequence
Length = 843
Score = 137 bits (293), Expect(3) = 3e-065
Identities = 51/93 (54%), Positives = 62/93 (66%)
Frame = +1 / +3
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDE 495
C T F M+KI L+ +L++YWPSLYC + TCF G+G FWAHEW HGTC P +QDE
Sbjct: 249 CRPTTTFNMNKIAMLRPILERYWPSLYCGDTSTCFGGRGPFWAHEWATHGTCGYPEIQDE 428
Query: 496 LQYFTLALDLYSKYNVTEMLSSGGIQVSNGKEY 594
YF+ AL LYSKYNVT+ L I NG +Y
Sbjct: 429 YDYFSTALYLYSKYNVTKALRKAHIYPRNGMKY 527
Score = 122 bits (262), Expect(3) = 3e-065
Identities = 40/60 (66%), Positives = 46/60 (76%)
Frame = +1 / +3
Query: 151 QREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCRRT 330
Q FDY+ L+LQWPG++C T HCC++NGCCRS PL FTIHGLWP Y G WPSCCR T
Sbjct: 81 QAGFDYYVLALQWPGSVCRQTNHCCSSNGCCRSNPLNWFTIHGLWPQYSYGGWPSCCRPT 260
Score = 66.6 bits (139), Expect(2) = 2e-022
Identities = 31/57 (54%), Positives = 32/57 (56%)
Frame = -3 / -3
Query: 320 QHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMVPGHWRESAK*SNSRC 150
Q DG P G SPWIVN G +RQQPL QQW V Q PGH R S *SN C
Sbjct: 250 QQDGHPP*LYCGQSPWIVNQLRGFDRQQPLELQQWLVWRQTEPGHCRAST**SNPAC 80
Score = 59.3 bits (123), Expect(2) = 2e-022
Identities = 41/89 (46%), Positives = 46/89 (51%)
Frame = -3 / -3
Query: 593 YSLPFETCIPPLDSISVTLYLE*RSRARVKYCNSS*TTGAEQVPCFSHS*AQKSPLPLKH 414
Y +PF + S VTLYL *R A K SS +G VP +HS AQK PLP K
Sbjct: 526 YFMPFRG*MCAFLSAFVTLYLL*R*SAVEK*SYSSCISG*PHVP*VAHSWAQKGPLPPKQ 347
Query: 413 VPDLEQ*RDGQYLSSTSFSGNILSISNWV 327
V Q* DGQYLS S IL + N V
Sbjct: 346 VEVSPQ*SDGQYLSRIGLSIAILFMLNVV 260
Score = 28.6 bits (56), Expect(3) = 3e-065
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +1 / +2
Query: 619 IKHAFGGSPQIVCKRGSIE 675
I HAFG +VCK GS++
Sbjct: 554 IDHAFGRLAHLVCKNGSLQ 610
>gb|BU998770.1|BU998770 HI12B24r HI Hordeum vulgare subsp. vulgare cDNA clone HI12B24
5-PRIME, mRNA sequence
Length = 543
Score = 134 bits (288), Expect(2) = 5e-061
Identities = 50/91 (54%), Positives = 61/91 (67%)
Frame = +1 / +1
Query: 316 CCRRTQFEMDKILPLKEVLDKYWPSLYCSKSGTCFSGKGLFWAHEWEKHGTCSAPVVQDE 495
C T F M+KI L+ +L++YWPSLYC + TCF G+G FWAHEW HGTC P +QDE
Sbjct: 271 CRPTTTFNMNKIAMLRPILERYWPSLYCGDTSTCFGGRGPFWAHEWATHGTCGYPEIQDE 450
Query: 496 LQYFTLALDLYSKYNVTEMLSSGGIQVSNGK 588
YF+ AL LYSKYNVT+ L I NG+
Sbjct: 451 YDYFSTALYLYSKYNVTKALRKAHIYPRNGR 543
Score = 120 bits (257), Expect(2) = 5e-061
Identities = 39/60 (65%), Positives = 46/60 (76%)
Frame = +1 / +1
Query: 151 QREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCRRT 330
Q FD++ L+LQWPG++C T HCC++NGCCRS PL FTIHGLWP Y G WPSCCR T
Sbjct: 103 QAGFDHYVLALQWPGSVCRQTNHCCSSNGCCRSNPLNWFTIHGLWPQYSYGGWPSCCRPT 282
Score = 68.0 bits (142), Expect(2) = 2e-022
Identities = 31/57 (54%), Positives = 32/57 (56%)
Frame = -3 / -2
Query: 320 QHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMVPGHWRESAK*SNSRC 150
Q DG P GHSPWIVN G +RQQPL QQW V Q PGH R S SN C
Sbjct: 272 QQDGHPP*LYCGHSPWIVNQLRGFDRQQPLELQQWLVWRQTEPGHCRAST*WSNPAC 102
Score = 57.9 bits (120), Expect(2) = 2e-022
Identities = 41/87 (47%), Positives = 45/87 (51%)
Frame = -3 / -2
Query: 587 LPFETCIPPLDSISVTLYLE*RSRARVKYCNSS*TTGAEQVPCFSHS*AQKSPLPLKHVP 408
LPF + S VTLYL *R A K SS +G VP +HS AQK PLP K V
Sbjct: 542 LPFRG*MCAFLSAFVTLYLL*R*SAVEK*SYSSCISG*PHVP*VAHSWAQKGPLPPKQVE 363
Query: 407 DLEQ*RDGQYLSSTSFSGNILSISNWV 327
Q* DGQYLS S IL + N V
Sbjct: 362 VSPQ*SDGQYLSRIGLSIAILFMLNVV 282
>gb|AV925297.1|AV925297 AV925297 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd22j13 5', mRNA sequence
Length = 471
Score = 225 bits (485), Expect = 5e-058
Identities = 90/117 (76%), Positives = 106/117 (90%)
Frame = +1 / +1
Query: 490 DELQYFTLALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVCKRGS 669
+ELQYF+ ALDLY KYNVTEML++G I VSNGKEYALSDVIDTIKHAFGGSPQI+CK+GS
Sbjct: 4 EELQYFSTALDLYFKYNVTEMLATGDILVSNGKEYALSDVIDTIKHAFGGSPQIICKKGS 183
Query: 670 IEELRLCFDKELKPRDCLTTSLANGSVSKSKHCPRYITLPTYDPLVLANSTVEIMTQ 840
++ELRLCF K+LKPRDCLTTS ++SKSKHCPR I+LPTYDP+VLANST+EI+ +
Sbjct: 184 VQELRLCFTKDLKPRDCLTTSAMYKNLSKSKHCPRKISLPTYDPIVLANSTLEIVPE 354
Score = 191 bits (411), Expect = 7e-048
Identities = 84/114 (73%), Positives = 94/114 (82%)
Frame = -3 / -2
Query: 830 ISTVELASTRGSYVGKVMYRGQCLLFDTLPFAKEVVRQSRGFNSLSKHNLSSSIEPLLQT 651
IS+VELAST GSYVG+ ++RGQC FD + EVVRQSRGF SL KHNLSS EP LQ
Sbjct: 344 ISSVELASTMGSYVGREIFRGQCFDFDRFLYIAEVVRQSRGFKSLVKHNLSS*TEPFLQM 165
Query: 650 ICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE*RSRARVKYCNSS 489
ICG+PPKACLMVSMTSLSAYSLP ET I P+ +ISVTLYL+*RSRA +KYCNSS
Sbjct: 164 ICGEPPKACLMVSMTSLSAYSLPLETRISPVANISVTLYLK*RSRAVLKYCNSS 3
Score = 126 bits (269), Expect = 3e-028
Identities = 61/114 (53%), Positives = 74/114 (64%)
Frame = +3 / +3
Query: 489 G*ITVFHPCP*PLLQV*RYGNAVKWRDTGFKW*GICTERCHRYHQTCFWGITTNCLQEGF 668
G ITV CP* LLQ+*R N WR G + * ICTE CH +HQ CFWG+T + LQEG
Sbjct: 3 GRITVLQHCP*SLLQI*RDRNVGDWRYPGLQR*RICTE*CH*HHQACFWGLTADHLQEGL 182
Query: 669 NRRT*VMLRQRIEAS*LPYHFFGEWKCIKKQALPAVHHLANV*SPCACQFNCRD 830
+ RT VML QR EAS*LPY+F + I+ +ALP+ + AN+*S QFN D
Sbjct: 183 SSRTKVMLHQRFEAS*LPYYFGNVQEPIEIKALPSKNLPANI*SHRTRQFNTGD 344
Score = 125 bits (268), Expect = 4e-028
Identities = 62/113 (54%), Positives = 79/113 (69%)
Frame = -2 / -1
Query: 831 DLYS*IGKHKGIIRWQGDVPRAVLAF*YTSIRQRSGKAITRLQFFVEA*PKFFD*TPLAN 652
+L *IG++ GII WQGD RA+L F + RS KAITRLQ F EA*P F +* LA+
Sbjct: 345 NLQC*IGEYDGIICWQGDFSRAML*FR*VLVHCRSSKAITRLQIFGEA*P*FLN*ALLAD 166
Query: 651 NLW*SPKSMFDGIDDIAQCIFLTI*NLYPST*QHFRNVILGVEVKGKGEIL*F 493
+L *+PKSM DG++DI QCIF T+ + S+ QHF +VI VE+KG E+L*F
Sbjct: 165 DLR*APKSMLDGVNDITQCIFFTVGDQDISSRQHFCHVIFEVEIKGSAEVL*F 7
Score = 104 bits (221), Expect = 1e-021
Identities = 57/111 (51%), Positives = 66/111 (59%)
Frame = -1 / -3
Query: 820 LNWQAQGDHTLAR*CTAGSACFLIHFHSPKKW*GNHEASILCRSIT*VLRLNPSCKQFVV 641
LNW+ + DH LA G+A I + K *GNHEAS L SIT VL L+PSC+ V
Sbjct: 334 LNWRVRWDHMLAGRFFEGNALISIGSCTLPK**GNHEASNLW*SITLVLELSPSCR*SAV 155
Query: 640 IPQKHV*WYR*HRSVHIPYHLKPVSLHLTAFP*RYTWSRGQGQG*NTVIHP 488
PQKH *W +*H SVHI Y +P L F RY SR QGQ *+TVI P
Sbjct: 154 SPQKHA*WCQ*HHSVHILYRWRPGYLQSPTFLSRYI*SRDQGQC*STVILP 2
>gb|AV930451.1|AV930451 AV930451 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd22j13 3', mRNA sequence
Length = 595
Score = 225 bits (485), Expect = 5e-058
Identities = 90/117 (76%), Positives = 106/117 (90%)
Frame = +1 / -1
Query: 490 DELQYFTLALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVCKRGS 669
+ELQYF+ ALDLY KYNVTEML++G I VSNGKEYALSDVIDTIKHAFGGSPQI+CK+GS
Sbjct: 592 EELQYFSTALDLYFKYNVTEMLATGDILVSNGKEYALSDVIDTIKHAFGGSPQIICKKGS 413
Query: 670 IEELRLCFDKELKPRDCLTTSLANGSVSKSKHCPRYITLPTYDPLVLANSTVEIMTQ 840
++ELRLCF K+LKPRDCLTTS ++SKSKHCPR I+LPTYDP+VLANST+EI+ +
Sbjct: 412 VQELRLCFTKDLKPRDCLTTSAMYKNLSKSKHCPRKISLPTYDPIVLANSTLEIVPE 242
Score = 191 bits (411), Expect = 7e-048
Identities = 84/114 (73%), Positives = 94/114 (82%)
Frame = -3 / +3
Query: 830 ISTVELASTRGSYVGKVMYRGQCLLFDTLPFAKEVVRQSRGFNSLSKHNLSSSIEPLLQT 651
IS+VELAST GSYVG+ ++RGQC FD + EVVRQSRGF SL KHNLSS EP LQ
Sbjct: 252 ISSVELASTMGSYVGREIFRGQCFDFDRFLYIAEVVRQSRGFKSLVKHNLSS*TEPFLQM 431
Query: 650 ICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE*RSRARVKYCNSS 489
ICG+PPKACLMVSMTSLSAYSLP ET I P+ +ISVTLYL+*RSRA +KYCNSS
Sbjct: 432 ICGEPPKACLMVSMTSLSAYSLPLETRISPVANISVTLYLK*RSRAVLKYCNSS 593
Score = 126 bits (269), Expect = 3e-028
Identities = 61/114 (53%), Positives = 74/114 (64%)
Frame = +3 / -3
Query: 489 G*ITVFHPCP*PLLQV*RYGNAVKWRDTGFKW*GICTERCHRYHQTCFWGITTNCLQEGF 668
G ITV CP* LLQ+*R N WR G + * ICTE CH +HQ CFWG+T + LQEG
Sbjct: 593 GRITVLQHCP*SLLQI*RDRNVGDWRYPGLQR*RICTE*CH*HHQACFWGLTADHLQEGL 414
Query: 669 NRRT*VMLRQRIEAS*LPYHFFGEWKCIKKQALPAVHHLANV*SPCACQFNCRD 830
+ RT VML QR EAS*LPY+F + I+ +ALP+ + AN+*S QFN D
Sbjct: 413 SSRTKVMLHQRFEAS*LPYYFGNVQEPIEIKALPSKNLPANI*SHRTRQFNTGD 252
Score = 125 bits (268), Expect = 4e-028
Identities = 62/113 (54%), Positives = 79/113 (69%)
Frame = -2 / +2
Query: 831 DLYS*IGKHKGIIRWQGDVPRAVLAF*YTSIRQRSGKAITRLQFFVEA*PKFFD*TPLAN 652
+L *IG++ GII WQGD RA+L F + RS KAITRLQ F EA*P F +* LA+
Sbjct: 251 NLQC*IGEYDGIICWQGDFSRAML*FR*VLVHCRSSKAITRLQIFGEA*P*FLN*ALLAD 430
Query: 651 NLW*SPKSMFDGIDDIAQCIFLTI*NLYPST*QHFRNVILGVEVKGKGEIL*F 493
+L *+PKSM DG++DI QCIF T+ + S+ QHF +VI VE+KG E+L*F
Sbjct: 431 DLR*APKSMLDGVNDITQCIFFTVGDQDISSRQHFCHVIFEVEIKGSAEVL*F 589
Score = 104 bits (221), Expect = 1e-021
Identities = 57/111 (51%), Positives = 66/111 (59%)
Frame = -1 / +1
Query: 820 LNWQAQGDHTLAR*CTAGSACFLIHFHSPKKW*GNHEASILCRSIT*VLRLNPSCKQFVV 641
LNW+ + DH LA G+A I + K *GNHEAS L SIT VL L+PSC+ V
Sbjct: 262 LNWRVRWDHMLAGRFFEGNALISIGSCTLPK**GNHEASNLW*SITLVLELSPSCR*SAV 441
Query: 640 IPQKHV*WYR*HRSVHIPYHLKPVSLHLTAFP*RYTWSRGQGQG*NTVIHP 488
PQKH *W +*H SVHI Y +P L F RY SR QGQ *+TVI P
Sbjct: 442 SPQKHA*WCQ*HHSVHILYRWRPGYLQSPTFLSRYI*SRDQGQC*STVILP 594
>gb|AL507460.1|AL507460 AL507460 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY05N03V 5',
mRNA sequence
Length = 699
Score = 211 bits (455), Expect = 6e-054
Identities = 85/110 (77%), Positives = 99/110 (90%)
Frame = +1 / -2
Query: 511 LALDLYSKYNVTEMLSSGGIQVSNGKEYALSDVIDTIKHAFGGSPQIVCKRGSIEELRLC 690
L LDLY KYNVTEML++G I VSNGKEYALSDVIDTIKHAFGGSPQI+CK+GS++ELRLC
Sbjct: 623 LPLDLYFKYNVTEMLATGDILVSNGKEYALSDVIDTIKHAFGGSPQIICKKGSVQELRLC 444
Query: 691 FDKELKPRDCLTTSLANGSVSKSKHCPRYITLPTYDPLVLANSTVEIMTQ 840
F K+LKPRDCLTTS ++SKSKHCPR I+LPTYDP+VLANST+EI+ +
Sbjct: 443 FTKDLKPRDCLTTSAMYKNLSKSKHCPRKISLPTYDPIVLANSTLEIVPE 294
Score = 176 bits (379), Expect = 2e-043
Identities = 78/107 (72%), Positives = 87/107 (81%)
Frame = -3 / +1
Query: 830 ISTVELASTRGSYVGKVMYRGQCLLFDTLPFAKEVVRQSRGFNSLSKHNLSSSIEPLLQT 651
IS+VELAST GSYVG+ ++RGQC FD + EVVRQSRGF SL KHNLSS EP LQ
Sbjct: 304 ISSVELASTMGSYVGREIFRGQCFDFDRFLYIAEVVRQSRGFKSLVKHNLSS*TEPFLQM 483
Query: 650 ICGDPPKACLMVSMTSLSAYSLPFETCIPPLDSISVTLYLE*RSRAR 510
ICG+PPKACLMVSMTSLSAYSLP ET I P+ +ISVTLYL+*RSR R
Sbjct: 484 ICGEPPKACLMVSMTSLSAYSLPLETRISPVANISVTLYLK*RSRGR 624
Score = 119 bits (254), Expect = 3e-026
Identities = 57/107 (53%), Positives = 70/107 (65%)
Frame = +3 / -1
Query: 510 PCP*PLLQV*RYGNAVKWRDTGFKW*GICTERCHRYHQTCFWGITTNCLQEGFNRRT*VM 689
P P* LLQ+*R N WR G + * ICTE CH +HQ CFWG+T + LQEG + RT VM
Sbjct: 624 PAP*SLLQI*RDRNVGDWRYPGLQR*RICTE*CH*HHQACFWGLTADHLQEGLSSRTKVM 445
Query: 690 LRQRIEAS*LPYHFFGEWKCIKKQALPAVHHLANV*SPCACQFNCRD 830
L QR EAS*LPY+F + I+ +ALP+ + AN+*S QFN D
Sbjct: 444 LHQRFEAS*LPYYFGNVQEPIEIKALPSKNLPANI*SHRTRQFNTGD 304
Score = 117 bits (250), Expect = 1e-025
Identities = 58/107 (54%), Positives = 75/107 (70%)
Frame = -2 / +3
Query: 831 DLYS*IGKHKGIIRWQGDVPRAVLAF*YTSIRQRSGKAITRLQFFVEA*PKFFD*TPLAN 652
+L *IG++ GII WQGD RA+L F + RS KAITRLQ F EA*P F +* LA+
Sbjct: 303 NLQC*IGEYDGIICWQGDFSRAML*FR*VLVHCRSSKAITRLQIFGEA*P*FLN*ALLAD 482
Query: 651 NLW*SPKSMFDGIDDIAQCIFLTI*NLYPST*QHFRNVILGVEVKGK 511
+L *+PKSM DG++DI QCIF T+ + S+ QHF +VI VE+KG+
Sbjct: 483 DLR*APKSMLDGVNDITQCIFFTVGDQDISSRQHFCHVIFEVEIKGQ 623
>gb|BF629887.2|BF629887 HVSMEb0007D12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0007D12f, mRNA sequence
Length = 502
Score = 130 bits (279), Expect(2) = 1e-039
Identities = 48/66 (72%), Positives = 50/66 (75%)
Frame = +1 / +2
Query: 145 KPQREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCR 324
K QREFDYFAL+LQ PG IC TRHCCA N CCR EP+ TFTIH LWPDYDD TWPSC
Sbjct: 179 KEQREFDYFALALQRPGAICFFTRHCCAVNSCCRMEPVHTFTIHWLWPDYDDWTWPSCLH 358
Query: 325 RTQFEM 342
T F M
Sbjct: 359 LTSFLM 376
Score = 104 bits (222), Expect(2) = 5e-028
Identities = 46/66 (69%), Positives = 50/66 (75%)
Frame = -3 / -2
Query: 347 LSISNWVRRQHDGQVPSS*SGHSPWIVNVWSGSERQQPLVAQQWRVEAQMVPGHWRESAK 168
L + VR +HDGQV SS*SGHS WIVNVW+GS RQQ L AQQWRV+ QM PG SAK
Sbjct: 381 LPMRKLVRCKHDGQVQSS*SGHSQWIVNVWTGSIRQQLLTAQQWRVKKQMAPGLCSASAK 202
Query: 167 *SNSRC 150
*SNSRC
Sbjct: 201 *SNSRC 184
Score = 53.3 bits (110), Expect(2) = 1e-039
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = +1 / +2
Query: 379 YWPSLYCSKSGTCFSGKGLFWA 444
YWPSLYCS S TCFSG G FWA
Sbjct: 416 YWPSLYCSSSSTCFSGMGPFWA 481
Score = 40.5 bits (82), Expect(2) = 5e-028
Identities = 16/23 (69%), Positives = 19/23 (82%)
Frame = -3 / -2
Query: 443 AQKSPLPLKHVPDLEQ*RDGQYL 375
AQK P+PLK V + EQ*RDGQY+
Sbjct: 480 AQKGPIPLKQVEEDEQ*RDGQYI 412
>gb|AV910880.1|AV910880 AV910880 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak11d09 3', mRNA sequence
Length = 232
Score = 156 bits (335), Expect = 2e-037
Identities = 61/75 (81%), Positives = 69/75 (92%)
Frame = +1 / -3
Query: 574 VSNGKEYALSDVIDTIKHAFGGSPQIVCKRGSIEELRLCFDKELKPRDCLTTSLANGSVS 753
VSNGKEYALSDVIDTIKHAFGGSPQI+CK+GS++ELRLCF K+LKPRDCLTTS ++S
Sbjct: 227 VSNGKEYALSDVIDTIKHAFGGSPQIICKKGSVQELRLCFTKDLKPRDCLTTSAMYKNLS 48
Query: 754 KSKHCPRYITLPTYD 798
KSKHCPR I+LPTYD
Sbjct: 47 KSKHCPRKISLPTYD 3
Score = 131 bits (281), Expect = 6e-030
Identities = 55/76 (72%), Positives = 60/76 (78%)
Frame = -3 / +1
Query: 800 GSYVGKVMYRGQCLLFDTLPFAKEVVRQSRGFNSLSKHNLSSSIEPLLQTICGDPPKACL 621
GSYVG+ ++RGQC FD + EVVRQSRGF SL KHNLSS EP LQ ICG+PPKACL
Sbjct: 1 GSYVGREIFRGQCFDFDRFLYIAEVVRQSRGFKSLVKHNLSS*TEPFLQMICGEPPKACL 180
Query: 620 MVSMTSLSAYSLPFET 573
MVSMTSL AYSLP ET
Sbjct: 181 MVSMTSLXAYSLPLET 228
>gb|CA027968.1|CA027968 HZ60J12r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ60J12
5-PRIME, mRNA sequence
Length = 422
Score = 120 bits (257), Expect = 1e-026
Identities = 39/60 (65%), Positives = 46/60 (76%)
Frame = +1 / +2
Query: 151 QREFDYFALSLQWPGTICASTRHCCATNGCCRSEPLQTFTIHGLWPDYDDGTWPSCCRRT 330
Q FD++ L+LQWPG++C T HCC++NGCCRS PL FTIHGLWP Y G WPSCCR T
Sbjct: 200 QAGFDHYVLALQWPGSVCRQTNHCCSSNGCCRSNPLNWFTIHGLWPQYSYGGWPSCCRPT 379
>gb|BQ665780.1|BQ665780 HZ01B14u HZ Hordeum vulgare subsp. vulgare cDNA clone HZ01B14
3-PRIME, mRNA sequence
Length = 462
Score = 104 bits (221), Expect = 1e-021
Identities = 40/57 (70%), Positives = 50/57 (87%)
Frame = +1 / -3
Query: 670 IEELRLCFDKELKPRDCLTTSLANGSVSKSKHCPRYITLPTYDPLVLANSTVEIMTQ 840
++ELRLCF K+LKPRDCLTTS ++SKSKHCPR I+LPTYDP+VLANST+EI+ +
Sbjct: 460 VQELRLCFTKDLKPRDCLTTSAMYKNLSKSKHCPRKISLPTYDPIVLANSTLEIVPE 290
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 487,389,103
Number of Sequences: 312970
Number of extensions: 8137522
Number of successful extensions: 61186
Number of sequences better than 1.0e-020: 24
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 13581999925
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)