TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521569.2.1
(887 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Li... 375 e-121
gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedl... 359 e-100
gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA li... 246 1e-064
gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-a... 232 2e-064
gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, n... 219 1e-063
gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. v... 238 3e-062
gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. v... 217 8e-056
gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA li... 170 1e-041
gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subs... 136 1e-038
gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. v... 129 9e-037
gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachi... 151 4e-036
gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Le... 141 4e-033
gb|BF254204.2|BF254204 HVSMEf0003E24f Hordeum vulgare seedl... 75 1e-030
gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA li... 128 3e-029
gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA li... 123 1e-027
>gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cD.08, mRNA sequence
Length = 1501
Score = 375 bits (813), Expect(2) = e-121
Identities = 160/175 (91%), Positives = 166/175 (94%)
Frame = -1 / +2
Query: 830 TRSVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSRIGGQLEMKQTPGS 651
T SVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSR GGQ+EMK PGS
Sbjct: 122 T*SVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSRPGGQIEMKNPPGS 301
Query: 650 PEAHTLARWIGRSYRFHTGAELLRTDTQCTDASLKALWQHSDSIMCCSLKAAPVFTFANQ 471
PEAHTLARWIGRSYRFHTGAELL T++Q DASLKALWQHSDSIMCCSLKAAPVFTFANQ
Sbjct: 302 PEAHTLARWIGRSYRFHTGAELLCTESQSADASLKALWQHSDSIMCCSLKAAPVFTFANQ 481
Query: 470 AGLDMLETTLIALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMG 306
AGLDMLETTLIALQDISLEKILDDDGRKALC+E+P IMQQGFAYLP GVCVSS+G
Sbjct: 482 AGLDMLETTLIALQDISLEKILDDDGRKALCSEFPXIMQQGFAYLPXGVCVSSIG 646
Score = 341 bits (738), Expect(2) = e-108
Identities = 149/175 (85%), Positives = 154/175 (88%)
Frame = +1 / -2
Query: 307 PMLDTHTPPGRYAKPCCIILGYSVQSAFLPSSSRIFSREMSWRAISVVSSMSRPAWLAKV 486
PMLDTHTP GRYAKPCC+I G S SA PSSSRIFS EMSW AISVVSSMSRPAWLAKV
Sbjct: 645 PMLDTHTPXGRYAKPCCMIXGNSEHSALRPSSSRIFSSEMSWSAISVVSSMSRPAWLAKV 466
Query: 487 NTGAAFREQHMIESECCHSAFKEASVHWVSVRRSSAPVWNL*LLPIHLASVCASGDPGVC 666
NTGAAFREQHMIESECCHSAF++AS SV+RSSAPV NL*LLPIHLASV ASGDPG
Sbjct: 465 NTGAAFREQHMIESECCHSAFRDASALCDSVQRSSAPV*NL*LLPIHLASV*ASGDPGGF 286
Query: 667 FISSCPPMREGAIAMATLCTDATTVLTYCLAMAATLSRR*FSYENWKAMVSTDLV 831
FIS CPP REGAIAMATLCTDATTVLTYCLA+AATLSRR FSYENWKAMVSTD V
Sbjct: 285 FISICPPGREGAIAMATLCTDATTVLTYCLAIAATLSRRWFSYENWKAMVSTDQV 121
Score = 264 bits (572), Expect(2) = 2e-074
Identities = 114/164 (69%), Positives = 131/164 (79%)
Frame = +3 / -1
Query: 336 EVREALLHNLRVLGAERLPAIVVKDLLKGDVLESNQRRLQHVQAGLVGKGEHGSSLQGTA 515
EVRE LLH+ LGA+RL AI+V+DLL+ DVLE NQRRLQHVQAGLVG+GEH S LQGTA
Sbjct: 616 EVRETLLHDXGELGAQRLAAIIVEDLLQRDVLERNQRRLQHVQAGLVGEGEHRSCLQGTA 437
Query: 516 HDRVRVLPQRFQGSIRALGVRAEEFGSSVEPVAPADPPCQRVCLRRSWSLLHFKLSTNAR 695
HD +RVLPQRFQG +RAL +RA+EF V+PVAPADPP +RV LR SW +LHF LST
Sbjct: 436 HD*IRVLPQRFQGCVRAL*LRAKEFRPGVKPVAPADPPRKRVSLRGSWRVLHFDLSTRTG 257
Query: 696 RSYCHGYPLHRCHHGPDVLPGHGCDALS*VIFI*ELESNGQH*S 827
RS CHG+PLH CHH P +LP H D LS V+ I*ELESNG+H*S
Sbjct: 256 RSDCHGHPLH*CHHSPHILPRHCRDTLSKVVLI*ELESNGKH*S 125
Score = 248 bits (537), Expect(2) = 1e-066
Identities = 108/168 (64%), Positives = 122/168 (72%)
Frame = -3 / +1
Query: 825 ISADHCFPVLI*KSPTRERRSHGQAIRQDRGGIGAEGSHGNSSFAHWWTA*NEANSRIS* 646
ISA HCFPVLI*+ P+RE R +G+A+ +D GGI AEG HGN SF WWT NE SRI *
Sbjct: 127 ISAYHCFPVLI*EPPSRECRGNGEAVCEDCGGISAEGGHGNRSFPSWWTDRNEEPSRIP* 306
Query: 645 GTHAGKVDRQELQVPHWSRTPPHGHPMHGCFLESAVATLGLDHVLFPEGCSRVHLCQPSR 466
G+HA +VDRQELQV H TP HG G ESAVA LG +HVLFPEG S VHL QP R
Sbjct: 307 GSHACEVDRQELQVSHRGGTPLHGVTERGRIPESAVAALGFNHVLFPEGSSCVHLRQPGR 486
Query: 465 PGHAGDDADCSPGHLP*EDP*RRWQEGALHRVP*DYATGLRVPPRWRV 322
PGHAGDDADC+PGHL EDP R W +GA+ RVP ++A G RVPP RV
Sbjct: 487 PGHAGDDADCAPGHLAGEDPRR*WPQGAVLRVPXNHAAGFRVPPXRRV 630
Score = 242 bits (522), Expect = 3e-063
Identities = 109/168 (64%), Positives = 119/168 (70%)
Frame = +2 / -3
Query: 323 TRHRGGTRSPVA*S*GTRCRAPSCHRRQGSSQGRCPGEQSASSPACPGRLGWQR*TREQP 502
TR GGTR+P A* GTR AP H R+GSS RCPG QSASSPACPGR GW+R*T+E P
Sbjct: 629 TRRXGGTRNPAA*XWGTRSTAPCGHHRRGSSPARCPGAQSASSPACPGRPGWRR*TQELP 450
Query: 503 SGNST*SSPSVATALSRKHPCIGCPCGGVRLQCGTCSSCRSTLPACVPQEILEFASFQAV 682
SGNST* +PS ATALS P PC GV +C TCSSCRST AC PQ ILE +SF++V
Sbjct: 449 SGNST*LNPSAATALSGMRPRSVTPCKGVPPRCETCSSCRSTSQACEPQGILEGSSFRSV 270
Query: 683 HQCAKELLPWLPSAPMPPRS*RIAWPWLRRSLVGDFHMRTGKQWSALI 826
HQ KE LPW PSA MPP+S A P R SL G HMRTGKQW ALI
Sbjct: 269 HQDGKERLPWPPSALMPPQSSHTASPLPRHSLEGGSHMRTGKQW*ALI 126
Score = 105 bits (225), Expect(4) = 4e-049
Identities = 45/65 (69%), Positives = 50/65 (76%)
Frame = -2 / +3
Query: 646 RHTRWQGGSAGATGSTLEPNSSARTPNARMLP*KRCGNTRTRSCAVP*RLLPCSPLPTKP 467
R TR +GGSAGATG T NS AR+ AR P*KRCG+TR +SCAVP*R L CSP PT+P
Sbjct: 306 RLTRLRGGSAGATGFTPGRNSFARSHRARTHP*KRCGSTRIQSCAVP*RQLLCSPSPTRP 485
Query: 466 AWTCW 452
AWTCW
Sbjct: 486 AWTCW 500
Score = 80.8 bits (170), Expect(2) = e-121
Identities = 31/36 (86%), Positives = 32/36 (88%)
Frame = -1 / +3
Query: 308 GRPVSYEQAVAWKVLSDDDTPHCLAFMFVNWSFV*E 201
GRPVSYEQAVAWKVL DDD PHCLAF VNWSFV*+
Sbjct: 645 GRPVSYEQAVAWKVLXDDDAPHCLAFHVVNWSFV*Q 752
Score = 79.4 bits (167), Expect(4) = 4e-049
Identities = 29/44 (65%), Positives = 34/44 (77%)
Frame = -2 / +3
Query: 826 DQC*PLLSSSHMKITYERASQPWPGNTSGPWWHRCRG*PWQ*LL 695
DQC PLLSSSHM+ T+ER S+ W G+ G WWH+CRG PWQ LL
Sbjct: 126 DQCLPLLSSSHMRTTFERVSRQWRGSM*GLWWHQCRGWPWQSLL 257
Score = 71.2 bits (149), Expect(2) = e-108
Identities = 28/35 (80%), Positives = 29/35 (82%)
Frame = +1 / -1
Query: 205 HTKDQFTNMKARQCGVSSSLRTFHATACSYDTGRP 309
+TKDQ T KARQCG SSS TFHATACSYDTGRP
Sbjct: 748 YTKDQLTT*KARQCGASSSXSTFHATACSYDTGRP 644
Score = 44.1 bits (90), Expect(4) = 4e-049
Identities = 18/29 (62%), Positives = 20/29 (68%)
Frame = -2 / +3
Query: 397 MAGRRSAPSTLRLCNRASRTSPVACVCRA 311
MA RR APS+ C+R SRTS AC CRA
Sbjct: 555 MAARRCAPSSPXSCSRVSRTSXAACACRA 641
Score = 35.0 bits (70), Expect(2) = 2e-074
Identities = 15/29 (51%), Positives = 20/29 (68%)
Frame = +3 / -3
Query: 180 STTRGTGFSHEGPVHEHEGKAVRGVVVAQ 266
++ G HEGPV + +G+AVR VVVAQ
Sbjct: 773 TSNAGYKLLHEGPVDDMKGEAVRRVVVAQ 687
Score = 24.9 bits (48), Expect(2) = 1e-066
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -2 / +1
Query: 247 RTALPSCS*TGPSCEN 200
RTA P S TGPSC +
Sbjct: 706 RTASPFMSSTGPSCSS 753
Score = 22.1 bits (42), Expect(4) = 4e-049
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -3 / +2
Query: 246 ALPCLHVRELVLRV 205
ALP L R+LVLRV
Sbjct: 707 ALPRLSCRQLVLRV 748
>gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001C22f, mRNA sequence
Length = 834
Score = 359 bits (779), Expect(2) = e-100
Identities = 147/162 (90%), Positives = 153/162 (94%)
Frame = -1 / -3
Query: 686 GGQLEMKQTPGSPEAHTLARWIGRSYRFHTGAELLRTDTQCTDASLKALWQHSDSIMCCS 507
GGQ+EMK PGS EAHTLARWIGRSYRFHTGAELL T++Q DASLKALWQHSDSIMCCS
Sbjct: 754 GGQIEMKNPPGSLEAHTLARWIGRSYRFHTGAELLCTESQSADASLKALWQHSDSIMCCS 575
Query: 506 LKAAPVFTFANQAGLDMLETTLIALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGG 327
LKAAPVFTFANQAGLDMLETTLIALQDISLEKILDDDGRKALC+E+PKIMQQGFAYLPGG
Sbjct: 574 LKAAPVFTFANQAGLDMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGG 395
Query: 326 VCVSSMGRPVSYEQAVAWKVLSDDDTPHCLAFMFVNWSFV*E 201
VCVSSM RPVSYEQAVAWKVL DDD PHCLAFMFVNWSFV*+
Sbjct: 394 VCVSSMRRPVSYEQAVAWKVLGDDDAPHCLAFMFVNWSFV*Q 269
Score = 312 bits (676), Expect(2) = 7e-085
Identities = 135/161 (83%), Positives = 139/161 (86%)
Frame = +1 / +3
Query: 205 HTKDQFTNMKARQCGVSSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQS 384
+TKDQ TNMKARQCG SSS TFHATACSYDTGR MLDTHTPPGRYAKPCC+ILG S S
Sbjct: 273 YTKDQLTNMKARQCGASSSPSTFHATACSYDTGRLMLDTHTPPGRYAKPCCMILGNSEHS 452
Query: 385 AFLPSSSRIFSREMSWRAISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFKEASV 564
A PSSSRIFS EMSW AISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAF+EAS
Sbjct: 453 ALRPSSSRIFSSEMSWSAISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFREASA 632
Query: 565 HWVSVRRSSAPVWNL*LLPIHLASVCASGDPGVCFISSCPP 687
SV+RSSAPV NL*LLPIHLASV AS DPG FIS CPP
Sbjct: 633 LCDSVQRSSAPV*NL*LLPIHLASV*ASRDPGGFFISICPP 755
Score = 197 bits (424), Expect(2) = 5e-057
Identities = 85/123 (69%), Positives = 100/123 (81%)
Frame = +3 / +2
Query: 336 EVREALLHNLRVLGAERLPAIVVKDLLKGDVLESNQRRLQHVQAGLVGKGEHGSSLQGTA 515
EVRE LLH+ LGA+RL AIVV+DLL+ DVLE NQRRLQHVQAGLVG+GEH S LQGTA
Sbjct: 404 EVRETLLHDFGELGAQRLAAIVVEDLLQRDVLERNQRRLQHVQAGLVGEGEHRSCLQGTA 583
Query: 516 HDRVRVLPQRFQGSIRALGVRAEEFGSSVEPVAPADPPCQRVCLRRSWSLLHFKLSTNAR 695
HD +RVLPQRFQGS+RAL +RA+EF V+PVAPADPP +RV L+ SW +LHF LST
Sbjct: 584 HD*IRVLPQRFQGSVRAL*LRAKEFRPGVKPVAPADPPRKRVSLKGSWRVLHFNLSTGRE 763
Query: 696 RSY 704
++
Sbjct: 764 SAF 772
Score = 188 bits (404), Expect(2) = 1e-050
Identities = 82/121 (67%), Positives = 90/121 (74%)
Frame = -3 / -2
Query: 684 WTA*NEANSRIS*GTHAGKVDRQELQVPHWSRTPPHGHPMHGCFLESAVATLGLDHVLFP 505
WT *NE SRI *G+HA +VDRQELQV H TP HG G F ESAVA LG +HVLFP
Sbjct: 752 WTD*NEEPSRIP*GSHACEVDRQELQVSHRGGTPLHGVTERGRFPESAVAALGFNHVLFP 573
Query: 504 EGCSRVHLCQPSRPGHAGDDADCSPGHLP*EDP*RRWQEGALHRVP*DYATGLRVPPRWR 325
EG S VHL QP RPGHAGDDADC+PGHL EDP RRW +GA+ RVP ++A G RVPPR R
Sbjct: 572 EGSSCVHLRQPGRPGHAGDDADCAPGHLAGEDPRRRWPQGAVLRVPQNHAAGFRVPPRRR 393
Query: 324 V 322
V
Sbjct: 392 V 390
Score = 187 bits (403), Expect = 7e-047
Identities = 83/125 (66%), Positives = 92/125 (73%)
Frame = +2 / +1
Query: 323 TRHRGGTRSPVA*S*GTRCRAPSCHRRQGSSQGRCPGEQSASSPACPGRLGWQR*TREQP 502
TR GGTR+P A* GTR AP HRR+GSS RCPG QSASSPACPGR GW+R*T+E P
Sbjct: 391 TRRLGGTRNPAA*FWGTRSTAPCGHRRRGSSPARCPGAQSASSPACPGRPGWRR*TQELP 570
Query: 503 SGNST*SSPSVATALSRKHPCIGCPCGGVRLQCGTCSSCRSTLPACVPQEILEFASFQAV 682
SGNST* +PS ATALS K P PC GV +C TCSSCRST AC PQ ILE +SFQ+V
Sbjct: 571 SGNST*LNPSAATALSGKRPRSVTPCKGVPPRCETCSSCRSTSQACEPQGILEGSSFQSV 750
Query: 683 HQCAK 697
H+ K
Sbjct: 751 HRTRK 765
Score = 145 bits (311), Expect(2) = 9e-037
Identities = 65/112 (58%), Positives = 73/112 (65%)
Frame = -2 / -1
Query: 646 RHTRWQGGSAGATGSTLEPNSSARTPNARMLP*KRCGNTRTRSCAVP*RLLPCSPLPTKP 467
R TR +GGSAGATG T NS AR+ AR LP*KRCG+TR +SCAVP*R L CSP PT+P
Sbjct: 714 RLTRLRGGSAGATGFTPGRNSFARSHRARTLP*KRCGSTRIQSCAVP*RQLLCSPSPTRP 535
Query: 466 AWTCWXXXXXXXXXXXXXXXXXTMAGRRSAPSTLRLCNRASRTSPVACVCRA 311
AWTCW TMA RR APS+ + C+R SRTS AC CRA
Sbjct: 534 AWTCWRRR*LRSRTSRWRRSSTTMAARRCAPSSPKSCSRVSRTSQAACACRA 379
Score = 44.6 bits (91), Expect(2) = 5e-057
Identities = 23/53 (43%), Positives = 31/53 (58%)
Frame = +3 / +2
Query: 108 TQAKATVHGVQSSNNRAANARQLGSTTRGTGFSHEGPVHEHEGKAVRGVVVAQ 266
T+ + T+ + S + R L ++ G HEGPV EHEG+AVR VVVAQ
Sbjct: 176 TERRHTMVFRRRSLQTNGSPRWLITSNAGYKLLHEGPVDEHEGEAVRRVVVAQ 334
Score = 32.2 bits (64), Expect(2) = 1e-050
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = -2 / -1
Query: 247 RTALPSCS*TGPSCEN 200
RTA PSCS TGPSC +
Sbjct: 315 RTASPSCSSTGPSCSS 268
Score = 28.6 bits (56), Expect(2) = 9e-037
Identities = 12/14 (85%), Positives = 13/14 (92%)
Frame = -3 / -2
Query: 246 ALPCLHVRELVLRV 205
ALP LHVR+LVLRV
Sbjct: 314 ALPRLHVRQLVLRV 273
Score = 26.3 bits (51), Expect(2) = e-100
Identities = 6/7 (85%), Positives = 7/7 (100%)
Frame = -2 / -1
Query: 736 WWHRCRG 716
WWH+CRG
Sbjct: 801 WWHQCRG 781
Score = 22.1 bits (42), Expect(2) = 7e-085
Identities = 12/26 (46%), Positives = 12/26 (46%)
Frame = +1 / +3
Query: 667 FISSCPPMREGAIAMATLCTDATTVL 744
F S P E LCTDAT VL
Sbjct: 732 FFISICPPDEKVHLPCHLCTDATKVL 809
>gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 3', mRNA sequence
Length = 659
Score = 246 bits (532), Expect = 1e-064
Identities = 95/151 (62%), Positives = 119/151 (78%)
Frame = -1 / -2
Query: 656 GSPEAHTLARWIGRSYRFHTGAELLRTDTQCTDASLKALWQHSDSIMCCSLKAAPVFTFA 477
G PEA TLARWI +SYRFH G EL R + ++ L+ LW H D+I+CCS K PVFTFA
Sbjct: 658 GFPEAATLARWICQSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFA 479
Query: 476 NQAGLDMLETTLIALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPV 297
N+ G++MLET+ +ALQD+SL+KI D+ GRKAL +E PK+M+QGF YLPGGVC+S MGR V
Sbjct: 478 NEMGINMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHV 299
Query: 296 SYEQAVAWKVLSDDDTPHCLAFMFVNWSFV* 204
S+E A+AWKV+ +D+ HCLAF FVNWSFV*
Sbjct: 298 SFENAIAWKVVGEDNNVHCLAFCFVNWSFV* 206
Score = 155 bits (332), Expect = 4e-037
Identities = 78/151 (51%), Positives = 88/151 (58%)
Frame = +1 / +3
Query: 205 HTKDQFTNMKARQCGVSSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQS 384
HTKDQ T KARQC + SS TFHA A S +T RP+ D HTPPGR KPC I G S S
Sbjct: 207 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 386
Query: 385 AFLPSSSRIFSREMSWRAISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFKEASV 564
AFLP+SS I S E S RA VS+M P A VNTG + +EQH I S S + S
Sbjct: 387 AFLPASSNILSNERS*RATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSP 566
Query: 565 HWVSVRRSSAPVWNL*LLPIHLASVCASGDP 657
+ +S P WN *L I ASV ASG P
Sbjct: 567 ASSACLNNSTPRWNR*LWHIQRASVAASGKP 659
>gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017A24f, mRNA sequence
Length = 905
Score = 232 bits (500), Expect(3) = 2e-064
Identities = 104/123 (84%), Positives = 107/123 (86%)
Frame = -1 / +3
Query: 866 TRASSDXXSTCNTRSVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSRI 687
TRA SD ST NTRSVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSR
Sbjct: 276 TRAPSDASSTSNTRSVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSRP 455
Query: 686 GGQLEMKQTPGSPEAHTLARWIGRSYRFHTGAELLRTDTQCTDASLKALWQHSDSIMCCS 507
GGQ+EMK GSPEAHTLARWIGRSY FHTGAELL T++Q DA L AL QHSDSIMC S
Sbjct: 456 GGQIEMKNPAGSPEAHTLARWIGRSYMFHTGAELLCTESQSADAYL*ALGQHSDSIMC*S 635
Query: 506 LKA 498
LKA
Sbjct: 636 LKA 644
Score = 215 bits (465), Expect(2) = 2e-056
Identities = 103/144 (71%), Positives = 113/144 (78%)
Frame = +1 / -2
Query: 436 AISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFKEASVHWVSVRRSSAPVWNL*L 615
A SV+S +A+ + AFR+QHMIESECC SA++ AS SV+ SSAPV N+*L
Sbjct: 706 AQSVLSLACSGRSVAEGDHSTAFRDQHMIESECCPSAYR*ASALCDSVQSSSAPV*NM*L 527
Query: 616 LPIHLASVCASGDPGVCFISSCPPMREGAIAMATLCTDATTVLTYCLAMAATLSRR*FSY 795
LPIHLASV ASGDP FIS CPP REGAIAMATLCTDATTVLTYCLA+AATLSRR FSY
Sbjct: 526 LPIHLASV*ASGDPAGFFISICPPGREGAIAMATLCTDATTVLTYCLAIAATLSRRWFSY 347
Query: 796 ENWKAMVSTDLVLQVLXXSLEARV 867
ENWKAMVSTDLVL VL SL ARV
Sbjct: 346 ENWKAMVSTDLVLLVLDASLGARV 275
Score = 180 bits (387), Expect = 1e-044
Identities = 79/121 (65%), Positives = 92/121 (76%)
Frame = +3 / -1
Query: 501 LQGTAHDRVRVLPQRFQGSIRALGVRAEEFGSSVEPVAPADPPCQRVCLRRSWSLLHFKL 680
LQG+AHD +RVLPQR Q S+RAL +RA++F V+ VAPADPP +RV LR S +LHF L
Sbjct: 641 LQGSAHD*IRVLPQRLQVSVRAL*LRAKQFRPGVKHVAPADPPRKRVSLRGSCRVLHFDL 462
Query: 681 STNARRSYCHGYPLHRCHHGPDVLPGHGCDALS*VIFI*ELESNGQH*SCVAGAXXVTRS 860
ST RR CHG+PLH CHH P +LP H D LS*V+ I*ELESNG+H*SCVAGA VT S
Sbjct: 461 STRTRRCNCHGHPLH*CHHSPHILPRHCRDTLS*VVLI*ELESNGKH*SCVAGAGCVTGS 282
Query: 861 T 863
T
Sbjct: 281 T 279
Score = 159 bits (341), Expect = 3e-038
Identities = 71/127 (55%), Positives = 85/127 (66%)
Frame = -3 / +2
Query: 867 NACFE*RXXHLQHKISADHCFPVLI*KSPTRERRSHGQAIRQDRGGIGAEGSHGNSSFAH 688
++C +*R H QHKISA HCFPVLI*+ PTRE R +G+A+ +D GGI AEG HGN +F+
Sbjct: 275 DSCSQ*RIQHQQHKISAYHCFPVLI*EPPTRECRGNGEAVCEDCGGISAEGGHGNCTFSS 454
Query: 687 WWTA*NEANSRIS*GTHAGKVDRQELQVPHWSRTPPHGHPMHGCFLESAVATLGLDHVLF 508
WWT NE RI *G+HA +VDRQEL V H T HG G SA A LG +HVL
Sbjct: 455 WWTDRNEEPCRIP*GSHACEVDRQELHVSHRGGTALHGVTERGRLPVSAGAALGFNHVLI 634
Query: 507 PEGCSRV 487
PEG + V
Sbjct: 635 PEGSTVV 655
Score = 158 bits (340), Expect = 3e-038
Identities = 72/121 (59%), Positives = 81/121 (66%)
Frame = +2 / -3
Query: 500 PSGNST*SSPSVATALSRKHPCIGCPCGGVRLQCGTCSSCRSTLPACVPQEILEFASFQA 679
PSG ST* +PS A AL+ K P PC V +C TCSSCRST AC PQ IL+ +SF++
Sbjct: 642 PSGIST*LNPSAAPALTGKRPRSVTPCKAVPPRCETCSSCRSTSQACEPQGILQGSSFRS 463
Query: 680 VHQCAKELLPWLPSAPMPPRS*RIAWPWLRRSLVGDFHMRTGKQWSALILCCRCXXRHSK 859
VHQ K LPW PSA MPP+S A P R SLVG HMRTGKQW ALILCC C RH +
Sbjct: 462 VHQDEKVQLPWPPSALMPPQSSHTASPLPRHSLVGGSHMRTGKQW*ALILCCWCWMRHWE 283
Query: 860 H 862
H
Sbjct: 282 H 280
Score = 96.4 bits (204), Expect(2) = 2e-029
Identities = 37/53 (69%), Positives = 41/53 (77%)
Frame = -2 / +1
Query: 862 VLRVTXXAPATQDQC*PLLSSSHMKITYERASQPWPGNTSGPWWHRCRG*PWQ 704
VL VT APATQDQC PLLSSSHM+ TYER S+ W G+ G WWH+CRG PWQ
Sbjct: 280 VLPVTHPAPATQDQCLPLLSSSHMRTTYERVSRQWRGSM*GLWWHQCRGWPWQ 438
Score = 52.8 bits (109), Expect(2) = 2e-029
Identities = 28/49 (57%), Positives = 32/49 (65%)
Frame = -2 / +1
Query: 646 RHTRWQGGSAGATGSTLEPNSSARTPNARMLP*KRCGNTRTRSCAVP*R 500
R TR +GGSAGAT T N AR+ AR L KR G+TR +SCA P*R
Sbjct: 496 RLTRLRGGSAGATCFTPGRNCFARSHRARTLTCKRWGSTRIQSCADP*R 642
Score = 28.1 bits (55), Expect(3) = 2e-064
Identities = 13/26 (50%), Positives = 15/26 (57%)
Frame = -1 / +2
Query: 440 IALQDISLEKILDDDGRKALCTEYPK 363
+ LQ KILDDDG + L YPK
Sbjct: 701 LRLQTCGW*KILDDDGPEDLGLRYPK 778
Score = 25.4 bits (49), Expect(3) = 2e-064
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -3 / +3
Query: 468 RPGHAGDDADCSPGHL 421
RP HA D+ DC+ H+
Sbjct: 672 RPEHARDNTDCASRHV 719
Score = 23.5 bits (45), Expect(2) = 2e-056
Identities = 11/23 (47%), Positives = 12/23 (52%)
Frame = +1 / -3
Query: 367 GYSVQSAFLPSSSRIFSREMSWR 435
GY + PSSSRIF WR
Sbjct: 774 GYRRPRSSGPSSSRIFYHPHVWR 706
>gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ004_N18 5', mRNA sequence
Length = 540
Score = 219 bits (473), Expect(2) = 1e-063
Identities = 88/121 (72%), Positives = 100/121 (82%)
Frame = -1 / +3
Query: 617 RSYRFHTGAELLRTDTQCTDASLKALWQHSDSIMCCSLKAAPVFTFANQAGLDMLETTLI 438
RSYR HTG E+ +DT+ D+ LK LW HSD+I+CCSLK AP+FTF N A LDMLETTL+
Sbjct: 3 RSYRAHTGEEIRWSDTEEADSPLKLLWNHSDAILCCSLKPAPMFTFGNNAALDMLETTLV 182
Query: 437 ALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQAVAWKVLSD 258
LQDISLE ILDD+GRKALC E+ K+MQQGFAYLPGGVC SSMGR SYEQAVAWKV+ D
Sbjct: 183 NLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAVAWKVVGD 362
Query: 257 D 255
D
Sbjct: 363 D 365
Score = 159 bits (342), Expect(2) = 2e-042
Identities = 77/122 (63%), Positives = 84/122 (68%)
Frame = +1 / -3
Query: 253 SSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQSAFLPSSSRIFSREMSW 432
+SS TFHATACSYD RPMLD HTPPGRYAKPCC+ L S SA PSSSRI S EMS
Sbjct: 367 TSSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMSC 188
Query: 433 RAISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFKEASVHWVSVRRSSAPVWNL* 612
R SVVSSMSR A L KVN GA FREQ + S H +F+ S VS +R S+PV L*
Sbjct: 187 RFTSVVSSMSRAALLPKVNIGAGFREQQSMASLWFHRSFRGESASSVSDQRISSPV*AL* 8
Query: 613 LL 618
LL
Sbjct: 7 LL 2
Score = 114 bits (244), Expect(2) = 7e-029
Identities = 53/95 (55%), Positives = 65/95 (68%)
Frame = +3 / -2
Query: 333 GEVREALLHNLRVLGAERLPAIVVKDLLKGDVLESNQRRLQHVQAGLVGKGEHGSSLQGT 512
GEVRE LLH+L LGA+RL +VV+D L+ DVL+ +QRRLQHV+ +V +GEH S LQ
Sbjct: 287 GEVRETLLHDLGELGAQRLAPLVVQDRLQRDVLQVHQRRLQHVKGRVVAEGEHRSRLQRA 108
Query: 513 AHDRVRVLPQRFQGSIRALGVRAEEFGSSVEPVAP 617
A V V+PQ QG IR GVR + S V PVAP
Sbjct: 107 AEYGVAVVPQELQGRIRFFGVRPTDLFSGVSPVAP 3
Score = 86.8 bits (183), Expect(2) = 2e-021
Identities = 47/99 (47%), Positives = 52/99 (52%)
Frame = -3 / +2
Query: 618 QELQVPHWSRTPPHGHPMHGCFLESAVATLGLDHVLFPEGCSRVHLCQPSRPGHAGDDAD 439
QELQ H R P GH G E+ V +L E CS VHL Q P HAGDDA
Sbjct: 2 QELQGSHRRRDPLVGHRRSGFSPEAPVEPQRRHTLLLSEACSDVHLRQQRGP*HAGDDAG 181
Query: 438 CSPGHLP*EDP*RRWQEGALHRVP*DYATGLRVPPRWRV 322
GHL DP RR +GA+ RV +A G RVPPR RV
Sbjct: 182 EPAGHLAGGDPGRRGAQGAVRRVLQGHAAGFRVPPRGRV 298
Score = 44.1 bits (90), Expect(2) = 1e-063
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -1 / +3
Query: 248 PHCLAFMFVNWSFV*EPGP 192
PHCLAFMFVNW+F+* P P
Sbjct: 378 PHCLAFMFVNWTFL*PPRP 434
Score = 35.4 bits (71), Expect(2) = 2e-021
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = -2 / +1
Query: 247 RTALPSCS*TGPSCE 203
RTALPSCS TGPSC+
Sbjct: 379 RTALPSCSSTGPSCD 423
Score = 33.6 bits (67), Expect(2) = 2e-042
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +3 / -2
Query: 204 SHEGPVHEHEGKAVR 248
S EGPV EHEGKAVR
Sbjct: 422 SQEGPVDEHEGKAVR 378
Score = 32.7 bits (65), Expect(2) = 7e-029
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +1 / -3
Query: 205 HTKDQFTNMKARQCGVSSS 261
H K Q TNMKARQCG ++
Sbjct: 421 HRKVQLTNMKARQCGAPAT 365
>gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
3-PRIME, mRNA sequence
Length = 638
Score = 238 bits (515), Expect = 3e-062
Identities = 92/147 (62%), Positives = 115/147 (78%)
Frame = -1 / -1
Query: 644 AHTLARWIGRSYRFHTGAELLRTDTQCTDASLKALWQHSDSIMCCSLKAAPVFTFANQAG 465
A TLARWI SYRFH G EL R + ++ L+ LW H D+I+CCS K PVFTFAN+ G
Sbjct: 638 AATLARWICHSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFANEMG 459
Query: 464 LDMLETTLIALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQ 285
++MLET+ +ALQD+SL+KI D+ GRKAL +E PK+M+QGF YLPGGVC+S MGR VS+E
Sbjct: 458 INMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFEN 279
Query: 284 AVAWKVLSDDDTPHCLAFMFVNWSFV* 204
A+AWKV+ +D+ HCLAF FVNWSFV*
Sbjct: 278 AIAWKVVGEDNNVHCLAFCFVNWSFV* 198
Score = 145 bits (312), Expect = 3e-034
Identities = 74/145 (51%), Positives = 84/145 (57%)
Frame = +1 / +1
Query: 205 HTKDQFTNMKARQCGVSSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQS 384
HTKDQ T KARQC + SS TFHA A S +T RP+ D HTPPGR KPC I G S S
Sbjct: 199 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 378
Query: 385 AFLPSSSRIFSREMSWRAISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFKEASV 564
AFLP+SS I S E S RA VS+M P A VNTG + +EQH I S S + S
Sbjct: 379 AFLPASSNILSNERS*RATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSP 558
Query: 565 HWVSVRRSSAPVWNL*LLPIHLASV 639
+ +S P WN *L I ASV
Sbjct: 559 ASSACLNNSTPRWNR*LWHIQRASV 633
>gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. vulgare cDNA clone HX01K23
5-PRIME, mRNA sequence
Length = 633
Score = 217 bits (468), Expect = 8e-056
Identities = 81/136 (59%), Positives = 105/136 (77%)
Frame = -1 / +1
Query: 617 RSYRFHTGAELLRTDTQCTDASLKALWQHSDSIMCCSLKAAPVFTFANQAGLDMLETTLI 438
+SYRFH G EL R + ++ L+ LW H D+I+CCS K PVFTFAN+ G++MLET+ +
Sbjct: 10 QSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFANEMGINMLETSFV 189
Query: 437 ALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQAVAWKVLSD 258
A QD+SL+KI D+ GRKAL +E PK+M+QGF YLPGGVC+S MGR VS+E A+AWKV+ +
Sbjct: 190 ATQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGE 369
Query: 257 DDTPHCLAFMFVNWSF 210
D+ HCLAF VNWSF
Sbjct: 370 DNNVHCLAFCLVNWSF 417
Score = 135 bits (290), Expect = 3e-031
Identities = 68/137 (49%), Positives = 79/137 (57%)
Frame = +1 / -2
Query: 205 HTKDQFTNMKARQCGVSSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQS 384
H+KDQ T KARQC + SS TFHA A S +T RP+ D HTPPGR KPC I G S S
Sbjct: 422 HSKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 243
Query: 385 AFLPSSSRIFSREMSWRAISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFKEASV 564
AFLP+SS I S E S A VS+M P A VNTG + +EQH I S S + S
Sbjct: 242 AFLPASSNILSNERS*VATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSP 63
Query: 565 HWVSVRRSSAPVWNL*L 615
+ +S P WN *L
Sbjct: 62 ASSACLNNSTPRWNR*L 12
>gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 5', mRNA sequence
Length = 633
Score = 170 bits (365), Expect = 1e-041
Identities = 71/123 (57%), Positives = 88/123 (71%)
Frame = -1 / +1
Query: 878 SGXTTRASSDXXSTCNTRSVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIA 699
+G TT +S CN RSVLTIAFQF YE HL++SVA MARQYVR++V++VQRV+MAI+
Sbjct: 265 AGSTTLQASGNADDCNLRSVLTIAFQFPYEMHLQDSVATMARQYVRSIVSAVQRVSMAIS 444
Query: 698 PSRIGGQLEMKQTPGSPEAHTLARWIGRSYRFHTGAELLRTDTQCTDASLKALWQHSDSI 519
PSR G E K G PEA TLARWI +SYRFH G EL R + ++ L+ LW H D+I
Sbjct: 445 PSRSGLNAEQKIISGFPEAATLARWICQSYRFHLGVELFRQADEAGESLLRMLWDHEDAI 624
Query: 518 MCC 510
+CC
Sbjct: 625 LCC 633
>gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200065F09F1, mRNA sequence
Length = 360
Score = 136 bits (291), Expect(2) = 1e-038
Identities = 55/68 (80%), Positives = 60/68 (88%)
Frame = -1 / +3
Query: 458 MLETTLIALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQAV 279
MLETTL+ LQDISLE ILDD+GRKALC E+ K+MQQGFAYLPGGVC SSMGR SYEQAV
Sbjct: 3 MLETTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAV 182
Query: 278 AWKVLSDD 255
AWKV+ DD
Sbjct: 183 AWKVVGDD 206
Score = 114 bits (243), Expect(2) = 5e-029
Identities = 50/69 (72%), Positives = 52/69 (75%)
Frame = +1 / -3
Query: 253 SSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQSAFLPSSSRIFSREMSW 432
+SS TFHATACSYD RPMLD HTPPGRYAKPCC+ L S SA PSSSRI S EMS
Sbjct: 208 TSSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMSC 29
Query: 433 RAISVVSSM 459
R SVVSSM
Sbjct: 28 RFTSVVSSM 2
Score = 44.1 bits (90), Expect(2) = 1e-038
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -1 / +3
Query: 248 PHCLAFMFVNWSFV*EPGP 192
PHCLAFMFVNW+F+* P P
Sbjct: 219 PHCLAFMFVNWTFL*PPRP 275
Score = 33.6 bits (67), Expect(2) = 5e-029
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +3 / -2
Query: 204 SHEGPVHEHEGKAVR 248
S EGPV EHEGKAVR
Sbjct: 263 SQEGPVDEHEGKAVR 219
>gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. vulgare cDNA clone HX01B05
3-PRIME, mRNA sequence
Length = 489
Score = 129 bits (277), Expect(2) = 9e-037
Identities = 52/65 (80%), Positives = 57/65 (87%)
Frame = -1 / -1
Query: 449 TTLIALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQAVAWK 270
TTL+ LQDISLE ILDD+GRKALC E+ K+MQQGFAYLPGGVC SSMGR SYEQAVAWK
Sbjct: 489 TTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAVAWK 310
Query: 269 VLSDD 255
V+ DD
Sbjct: 309 VVGDD 295
Score = 106 bits (226), Expect(2) = 1e-026
Identities = 46/65 (70%), Positives = 48/65 (73%)
Frame = +1 / +2
Query: 253 SSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQSAFLPSSSRIFSREMSW 432
+SS TFHATACSYD RPMLD HTPPGRYAKPCC+ L S SA PSSSRI S EMS
Sbjct: 293 TSSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMSC 472
Query: 433 RAISV 447
R SV
Sbjct: 473 RFTSV 487
Score = 44.1 bits (90), Expect(2) = 9e-037
Identities = 15/19 (78%), Positives = 17/19 (89%)
Frame = -1 / -1
Query: 248 PHCLAFMFVNWSFV*EPGP 192
PHCLAFMFVNW+F+* P P
Sbjct: 282 PHCLAFMFVNWTFL*PPRP 226
Score = 33.6 bits (67), Expect(2) = 1e-026
Identities = 13/15 (86%), Positives = 13/15 (86%)
Frame = +3 / +1
Query: 204 SHEGPVHEHEGKAVR 248
S EGPV EHEGKAVR
Sbjct: 238 SQEGPVDEHEGKAVR 282
>gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009M03f, mRNA sequence
Length = 718
Score = 151 bits (325), Expect = 4e-036
Identities = 63/72 (87%), Positives = 68/72 (94%)
Frame = -1 / +1
Query: 461 DMLETTLIALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQA 282
DMLETTLIALQDISLEKILDDDGRKALC+E+PKIMQQGFAYLPGGVCVSSMGRPVSYE A
Sbjct: 25 DMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGGVCVSSMGRPVSYEXA 204
Query: 281 VAWKVLSDDDTP 246
VA KVL++ + P
Sbjct: 205 VAXKVLAEGEAP 240
Score = 143 bits (306), Expect = 2e-033
Identities = 60/79 (75%), Positives = 63/79 (79%)
Frame = +1 / -3
Query: 229 MKARQCGVSSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQSAFLPSSSR 408
+ R G S S T HATACSYDTGRPMLDTHTPPGRYAKPCC+ILG S SA PSSSR
Sbjct: 257 LPVRYPGASPSASTXHATACSYDTGRPMLDTHTPPGRYAKPCCMILGNSEHSALRPSSSR 78
Query: 409 IFSREMSWRAISVVSSMSR 465
IFS EMSW AISVVSSMS+
Sbjct: 77 IFSSEMSWSAISVVSSMSQ 21
>gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 523
Score = 141 bits (303), Expect = 4e-033
Identities = 54/79 (68%), Positives = 67/79 (84%)
Frame = -1 / +3
Query: 440 IALQDISLEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQAVAWKVLS 261
+ALQD+SL+KI D+ GRKAL +E PK+M+QGF YLPGGVC+S MGR VS+E A+AWKV+
Sbjct: 6 VALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVG 185
Query: 260 DDDTPHCLAFMFVNWSFV* 204
+D+ HCLAF FVNWSFV*
Sbjct: 186 EDNNVHCLAFCFVNWSFV* 242
Score = 105 bits (225), Expect = 3e-022
Identities = 48/78 (61%), Positives = 52/78 (66%)
Frame = +1 / -1
Query: 205 HTKDQFTNMKARQCGVSSSLRTFHATACSYDTGRPMLDTHTPPGRYAKPCCIILGYSVQS 384
HTKDQ T KARQC + SS TFHA A S +T RP+ D HTPPGR KPC I G S S
Sbjct: 241 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 62
Query: 385 AFLPSSSRIFSREMSWRA 438
AFLP+SS I S E S RA
Sbjct: 61 AFLPASSNILSNERS*RA 8
>gb|BF254204.2|BF254204 HVSMEf0003E24f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0003E24f, mRNA sequence
Length = 860
Score = 75.3 bits (158), Expect(4) = 1e-030
Identities = 27/52 (51%), Positives = 46/52 (88%)
Frame = -1 / +3
Query: 842 STCNTRSVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSRI 687
S +++V+TIAFQF++E+HL++SVA MARQY+R++++SVQR+A+A++ S +
Sbjct: 360 SRSGSKAVMTIAFQFAFESHLQDSVAVMARQYMRSIISSVQRIALALSSSHL 515
Score = 47.8 bits (98), Expect(4) = 1e-030
Identities = 18/27 (66%), Positives = 23/27 (85%)
Frame = -1 / +3
Query: 659 PGSPEAHTLARWIGRSYRFHTGAELLR 579
P +PEA TL+RWI +SYRFH GAEL++
Sbjct: 546 PVTPEAATLSRWIVQSYRFHFGAELIK 626
Score = 40.9 bits (83), Expect(4) = 1e-030
Identities = 16/28 (57%), Positives = 23/28 (82%)
Frame = -1 / +3
Query: 485 TFANQAGLDMLETTLIALQDISLEKILD 402
TFANQ+GLDML TTL A + +++++LD
Sbjct: 720 TFANQSGLDMLXTTLAAPPEDTMDRVLD 803
Score = 24.4 bits (47), Expect(4) = 1e-030
Identities = 7/11 (63%), Positives = 10/11 (90%)
Frame = -1 / +2
Query: 533 HSDSIMCCSLK 501
H+ +I+CCSLK
Sbjct: 674 HTSAILCCSLK 706
>gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 3', mRNA sequence
Length = 420
Score = 128 bits (275), Expect = 3e-029
Identities = 49/72 (68%), Positives = 60/72 (83%)
Frame = -1 / -3
Query: 419 LEKILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQAVAWKVLSDDDTPHC 240
L+KI D+ GRKAL +E PK+M+QGF YLPGGVC+S MGR VS+E A+AWKV+ +D+ HC
Sbjct: 418 LDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHC 239
Query: 239 LAFMFVNWSFV* 204
LAF FVNWSFV*
Sbjct: 238 LAFCFVNWSFV* 203
>gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 5', mRNA sequence
Length = 447
Score = 123 bits (264), Expect = 1e-027
Identities = 47/69 (68%), Positives = 57/69 (82%)
Frame = -1 / +1
Query: 410 ILDDDGRKALCTEYPKIMQQGFAYLPGGVCVSSMGRPVSYEQAVAWKVLSDDDTPHCLAF 231
I D+ GRKAL +E PK+M+QGF YLPGGVC+S MGR VS+E A+AWKV+ +D+ HCLAF
Sbjct: 13 IFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHCLAF 192
Query: 230 MFVNWSFV* 204
FVNWSFV*
Sbjct: 193 CFVNWSFV* 219
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 503,012,226
Number of Sequences: 312970
Number of extensions: 10159543
Number of successful extensions: 108217
Number of sequences better than 1.0e-020: 21
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 10231208577
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)