TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521459.2.4
(760 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV912784.1|AV912784 AV912784 K. Sato unpublished cDNA li... 278 2e-093
gb|AV915960.1|AV915960 AV915960 K. Sato unpublished cDNA li... 323 9e-088
gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-a... 214 8e-057
gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa... 194 3e-053
gb|AV917519.1|AV917519 AV917519 K. Sato unpublished cDNA li... 159 9e-052
gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedl... 193 1e-048
gb|BG309831.1|BG309831 HVSMEc0013A09f Hordeum vulgare seedl... 160 6e-039
gb|BF631171.2|BF631171 HVSMEb0015C20f Hordeum vulgare seedl... 125 3e-028
gb|BU995988.1|BU995988 HM12A03r HM Hordeum vulgare subsp. v... 84 4e-027
gb|BF623091.2|BF623091 HVSMEa0014D14f Hordeum vulgare seedl... 106 2e-022
gb|BE455736.3|BE455736 HVSMEg0019G20f Hordeum vulgare pre-a... 75 2e-021
>gb|AV912784.1|AV912784 AV912784 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10e01 5', mRNA sequence
Length = 543
Score = 278 bits (601), Expect(3) = 2e-093
Identities = 112/127 (88%), Positives = 119/127 (93%)
Frame = -3 / +3
Query: 743 KLHAQVGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYFDELLPR 564
KLHA+VGRYK PDEHPFF +DLPE LPP+QYP VLHPIADSININKEIWKMYFDE++PR
Sbjct: 6 KLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEVIPR 185
Query: 563 LVKEGSDGNAGSSALCDTTCLQALSRRIHYGKFVAEAKFQESPEAYTPAIIAQDRDQLMN 384
LVKEGSDGN+GSSALCDTTCLQALS+RIHYGKFVAEAKFQESPEAYTPAI AQD DQLM
Sbjct: 186 LVKEGSDGNSGSSALCDTTCLQALSKRIHYGKFVAEAKFQESPEAYTPAIKAQDGDQLMQ 365
Query: 383 LLTYETV 363
LLTYETV
Sbjct: 366 LLTYETV 386
Score = 219 bits (472), Expect(3) = 2e-075
Identities = 102/127 (80%), Positives = 109/127 (85%)
Frame = +1 / -3
Query: 364 TVSYVRRFMS*SRSWAIMAGV*ASGDS*NLASATNFP*WILLESACKQVVSQRALDPALP 543
TVS VR +S*S S A +AGV*ASGDS*NLASATNFP*WILLESACKQVVSQRALDP P
Sbjct: 385 TVS*VRSCIS*SPS*AFIAGV*ASGDS*NLASATNFP*WILLESACKQVVSQRALDPEFP 206
Query: 544 SLPSFTNLGRSSSKYIFQISLLILIESAMGCKTLGYCIGGNRGSGRSLEKKGCSSGPLYL 723
SLPSFTNLG +SSKYIFQISLLIL+ESA+GC+T+GY IGGN SGRS K GCSSG LYL
Sbjct: 205 SLPSFTNLGITSSKYIFQISLLILMESAIGCRTVGYWIGGNSVSGRSSGKNGCSSGLLYL 26
Query: 724 PTCAWSF 744
PT A SF
Sbjct: 25 PTFACSF 5
Score = 200 bits (431), Expect(3) = 2e-060
Identities = 86/118 (72%), Positives = 94/118 (79%)
Frame = +2 / -1
Query: 392 VDHGLGLLWLACKLQGTLETWPLPQTSHSGSFWRVPASRLCHKEHWIQHYHHFLLSPILE 571
VDH LL CKLQGTLETWPLPQTSHSG F RVPASR CHKEHWIQ++HHFLLSP LE
Sbjct: 357 VDHRPELLLQVCKLQGTLETWPLPQTSHSGFF*RVPASRSCHKEHWIQNFHHFLLSPTLE 178
Query: 572 EVHQNTFSKSLC*Y**NQQWDAKPLGIA*VATGAQADPWKRKGAHLGPCISQPVHGAF 745
+HQ+TFSKSLC*Y* NQQ DA+PL * AT +QAD +R GAHLG CISQP+ AF
Sbjct: 177 *LHQSTFSKSLC*Y*WNQQ*DAEPLDTG*AATVSQADLLERMGAHLGSCISQPLRVAF 4
Score = 186 bits (401), Expect(3) = 7e-058
Identities = 84/118 (71%), Positives = 91/118 (77%)
Frame = -1 / +1
Query: 745 KSSMHRLGDTRAQMSTLSFPRICLSPGCHLCNTQGFCIPLLILSISTKRFGKCILMNFFQ 566
+S RLGDTRAQMST SF +ICL C L + Q FCI LLI ISTKRFGKC LM FQ
Sbjct: 4 ESYTQRLGDTRAQMSTHSFQKICLRHCCRLSSIQRFCILLLIPLISTKRFGKCTLMKLFQ 183
Query: 565 DW*KKEVMVMLDPVLFVTQPACRHSPEGSTMGSLWQRPSFKSPLKLTRQP**PKTVIN 392
W*KKEVM +LDPVLFVT+PACRHS + STMGSLWQRPSFKSPLKLT * +TVIN
Sbjct: 184 GW*KKEVMEILDPVLFVTRPACRHSLKESTMGSLWQRPSFKSPLKLTHLQ*KLRTVIN 357
Score = 185 bits (399), Expect(3) = 4e-053
Identities = 80/124 (64%), Positives = 88/124 (70%)
Frame = +3 / -2
Query: 366 RFICEKVHELITVLGYYGWRVSFRGLLKLGLCHKLPIVDPSGECLQAGCVXXXXXXXXXX 545
R + E++H LITVL +Y VSFRGLLKLGLCHKLPIVD ECLQAG V
Sbjct: 383 RLVGEELH*LITVLSFYCRCVSFRGLLKLGLCHKLPIVDSFRECLQAGRVTKSTGSRISI 204
Query: 546 XXFFHQSWKKFIKIHFPNLFVDIDRISNGMQNPWVLHRWQPGLRQILGKERVLIWALVSP 725
FFHQ W FIK+HFPNLFVDI+ ISN MQN W+L R Q LRQI KE VLIWALVSP
Sbjct: 203 TSFFHQPWNNFIKVHFPNLFVDINGISNRMQNRWILDRRQQCLRQIFWKEWVLIWALVSP 24
Query: 726 NLCM 737
NLC+
Sbjct: 23 NLCV 12
Score = 185 bits (399), Expect(3) = 2e-052
Identities = 85/127 (66%), Positives = 97/127 (76%)
Frame = -2 / +2
Query: 744 KAPCTGWEIQGPR*APFLFQGSA*APVATYAIPKGFASHC*FYQYQQRDLENVF**TSSK 565
KA GWEIQ PR*AP L + SA* VA Y + G AS+C*F+ YQQRDLENV **+ SK
Sbjct: 5 KATRKGWEIQEPR*APILSRRSA*DTVAAYPVSNGSASYC*FH*YQQRDLENVL**SYSK 184
Query: 564 IGERRK*W*CWIQCSL*HNLLAGTLQKDPLWEVCGRGQVSRVP*SLHASHNSPRP*STHE 385
+GERRK*W WIQCSL*H+LLAGTL K+PLWEVCGRGQVSRVP*SLH + S *ST+
Sbjct: 185 VGERRK*WKFWIQCSL*HDLLAGTL*KNPLWEVCGRGQVSRVP*SLHTCNKSSGR*STNA 364
Query: 384 PSHI*NG 364
H+ +G
Sbjct: 365 APHLRDG 385
Score = 58.3 bits (121), Expect(3) = 2e-093
Identities = 25/31 (80%), Positives = 25/31 (80%)
Frame = -3 / +1
Query: 362 ERAIEHRVEAKAKIFGQEVNIGVEDNGSPPV 270
ERAIEHRVE KAKIFGQEVNIG E G P V
Sbjct: 388 ERAIEHRVETKAKIFGQEVNIGAEAKGMPCV 480
Score = 57.9 bits (120), Expect(3) = 2e-075
Identities = 23/31 (74%), Positives = 26/31 (83%)
Frame = +1 / -2
Query: 271 TGGLPLSSTPMFTSCPKILALASTLCSIARS 363
T G+PL+S PMFTSCP ILAL ST CS+ARS
Sbjct: 479 TQGMPLASAPMFTSCPNILALVSTRCSMARS 387
Score = 45.5 bits (93), Expect(3) = 2e-093
Identities = 20/23 (86%), Positives = 20/23 (86%)
Frame = -3 / +2
Query: 275 PVYKIVPSLVAELYSYRIMPLTK 207
PVYKI PSLVA LYS RIMPLTK
Sbjct: 473 PVYKIRPSLVAGLYSNRIMPLTK 541
Score = 44.6 bits (91), Expect(3) = 2e-075
Identities = 20/24 (83%), Positives = 21/24 (87%)
Frame = +1 / -1
Query: 205 SLVSGMIL*LYSSATKLGTILYTG 276
SLVSGMIL LYS AT+LG ILYTG
Sbjct: 543 SLVSGMILLLYSPATRLGLILYTG 472
Score = 40.9 bits (83), Expect(3) = 7e-058
Identities = 16/22 (72%), Positives = 17/22 (77%)
Frame = -1 / +3
Query: 271 CTRSFRAWSPSCTATESCR*PK 206
CTRS AWS CTAT SCR*P+
Sbjct: 477 CTRSGPAWSLDCTATGSCR*PR 542
Score = 38.6 bits (78), Expect(3) = 2e-060
Identities = 17/22 (77%), Positives = 17/22 (77%)
Frame = +3 / -3
Query: 210 G*RHDSVAVQLGDQARNDLVHR 275
G*RHD VAVQ DQA DLVHR
Sbjct: 538 G*RHDPVAVQSSDQAGPDLVHR 473
Score = 35.4 bits (71), Expect(3) = 7e-058
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = -2 / +3
Query: 363 GACYRTQGGGQSQDLRARGEHR 298
GAC+RT GG Q D+ GEHR
Sbjct: 387 GACHRTSGGNQG*DIWTGGEHR 452
Score = 33.6 bits (67), Expect(3) = 4e-053
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = +2 / -2
Query: 209 WLAA*FCSCTARRPSSERSCT 271
WLAA* C CT +RP SCT
Sbjct: 539 WLAA*SCCCTVQRPGWA*SCT 477
Score = 32.2 bits (64), Expect(3) = 2e-052
Identities = 13/21 (61%), Positives = 17/21 (80%)
Frame = -2 / +1
Query: 270 VQDRSELGRRAVQLQNHAANQ 208
VQD+++ GR VQ Q+HAANQ
Sbjct: 478 VQDQAQPGRWTVQQQDHAANQ 540
Score = 32.2 bits (64), Expect(3) = 2e-060
Identities = 12/20 (60%), Positives = 14/20 (70%)
Frame = +3 / -1
Query: 303 VHLLPEDLGFGLHPVFDSTL 362
VHLL + L G HP+FD TL
Sbjct: 447 VHLLSKYLSLGFHPMFDGTL 388
Score = 27.6 bits (54), Expect(3) = 4e-053
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +2 / -3
Query: 302 CSPLARRSWLWPPPCVR*HAP 364
CSP + S W PP VR HAP
Sbjct: 448 CSPPVQIS*PWFPPDVRWHAP 386
Score = 26.7 bits (52), Expect(3) = 2e-052
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = -1 / +2
Query: 361 SVLSNTGWRPKPRSSGKR*T 302
SV SN GW+P+ R +R*T
Sbjct: 389 SVPSNIGWKPRLRYLDRR*T 448
>gb|AV915960.1|AV915960 AV915960 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16n12 5', mRNA sequence
Length = 470
Score = 323 bits (699), Expect = 9e-088
Identities = 132/151 (87%), Positives = 138/151 (91%)
Frame = -3 / +3
Query: 728 VGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYFDELLPRLVKEG 549
VGRYK PDEHPFF +DLPE LPP+QYP VLHPIADSININKEIWKMYFDE++PRLVKEG
Sbjct: 3 VGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEVIPRLVKEG 182
Query: 548 SDGNAGSSALCDTTCLQALSRRIHYGKFVAEAKFQESPEAYTPAIIAQDRDQLMNLLTYE 369
SDGN+GSSALCDTTCLQALS+RIHYGKFVAEAKFQESPEAYTPAI AQD DQLM LLTYE
Sbjct: 183 SDGNSGSSALCDTTCLQALSKRIHYGKFVAEAKFQESPEAYTPAIKAQDGDQLMQLLTYE 362
Query: 368 TVERAIEHRVEAKAKIFGQEVNIGVEDNGSP 276
TVERAIEHRVE KAKIFGQEVNIG E G P
Sbjct: 363 TVERAIEHRVETKAKIFGQEVNIGAEAKGMP 455
Score = 268 bits (579), Expect = 3e-071
Identities = 121/151 (80%), Positives = 131/151 (86%)
Frame = +1 / -2
Query: 277 GLPLSSTPMFTSCPKILALASTLCSIARSTVSYVRRFMS*SRSWAIMAGV*ASGDS*NLA 456
G+PL+S PMFTSCP ILAL ST CS+ARSTVS VR +S*S S A +AGV*ASGDS*NLA
Sbjct: 454 GMPLASAPMFTSCPNILALVSTRCSMARSTVS*VRSCIS*SPS*AFIAGV*ASGDS*NLA 275
Query: 457 SATNFP*WILLESACKQVVSQRALDPALPSLPSFTNLGRSSSKYIFQISLLILIESAMGC 636
SATNFP*WILLESACKQVVSQRALDP PSLPSFTNLG +SSKYIFQISLLIL+ESA+GC
Sbjct: 274 SATNFP*WILLESACKQVVSQRALDPEFPSLPSFTNLGITSSKYIFQISLLILMESAIGC 95
Query: 637 KTLGYCIGGNRGSGRSLEKKGCSSGPLYLPT 729
+T+GY IGGN SGRS K GCSSG LYLPT
Sbjct: 94 RTVGYWIGGNSVSGRSSGKNGCSSGLLYLPT 2
Score = 217 bits (468), Expect = 6e-056
Identities = 97/143 (67%), Positives = 106/143 (74%)
Frame = +2 / -3
Query: 302 CSPLARRSWLWPPPCVR*HAPPFHM*EGS*VDHGLGLLWLACKLQGTLETWPLPQTSHSG 481
CSP + S W PP VR HA P * + VDH LL CKLQGTLETWPLPQTSHSG
Sbjct: 429 CSPPVQIS*PWFPPDVRWHARPSRR*GAALVDHRPELLLQVCKLQGTLETWPLPQTSHSG 250
Query: 482 SFWRVPASRLCHKEHWIQHYHHFLLSPILEEVHQNTFSKSLC*Y**NQQWDAKPLGIA*V 661
F RVPASR CHKEHWIQ++HHFLLSP LE +HQ+TFSKSLC*Y* NQQ DA+PL *
Sbjct: 249 FF*RVPASRSCHKEHWIQNFHHFLLSPTLE*LHQSTFSKSLC*Y*WNQQ*DAEPLDTG*A 70
Query: 662 ATGAQADPWKRKGAHLGPCISQP 730
AT +QAD +R GAHLG CISQP
Sbjct: 69 ATVSQADLLERMGAHLGSCISQP 1
Score = 212 bits (458), Expect = 2e-054
Identities = 95/144 (65%), Positives = 109/144 (75%)
Frame = -2 / +2
Query: 729 GWEIQGPR*APFLFQGSA*APVATYAIPKGFASHC*FYQYQQRDLENVF**TSSKIGERR 550
GWEIQ PR*AP L + SA* VA Y + G AS+C*F+ YQQRDLENV **+ SK+GERR
Sbjct: 2 GWEIQEPR*APILSRRSA*DTVAAYPVSNGSASYC*FH*YQQRDLENVL**SYSKVGERR 181
Query: 549 K*W*CWIQCSL*HNLLAGTLQKDPLWEVCGRGQVSRVP*SLHASHNSPRP*STHEPSHI* 370
K*W WIQCSL*H+LLAGTL K+PLWEVCGRGQVSRVP*SLH + S *ST+ H+
Sbjct: 182 K*WKFWIQCSL*HDLLAGTL*KNPLWEVCGRGQVSRVP*SLHTCNKSSGR*STNAAPHLR 361
Query: 369 NGGACYRTQGGGQSQDLRARGEHR 298
+G AC+RT GG Q D+ GEHR
Sbjct: 362 DGRACHRTSGGNQG*DIWTGGEHR 433
Score = 208 bits (448), Expect = 4e-053
Identities = 90/142 (63%), Positives = 99/142 (69%)
Frame = +3 / -1
Query: 303 VHLLPEDLGFGLHPVFDSTLHRFICEKVHELITVLGYYGWRVSFRGLLKLGLCHKLPIVD 482
VHLL + L G HP+FD TL R + E++H LITVL +Y VSFRGLLKLGLCHKLPIVD
Sbjct: 428 VHLLSKYLSLGFHPMFDGTLDRLVGEELH*LITVLSFYCRCVSFRGLLKLGLCHKLPIVD 249
Query: 483 PSGECLQAGCVXXXXXXXXXXXXFFHQSWKKFIKIHFPNLFVDIDRISNGMQNPWVLHRW 662
ECLQAG V FFHQ W FIK+HFPNLFVDI+ ISN MQN W+L R
Sbjct: 248 SFRECLQAGRVTKSTGSRISITSFFHQPWNNFIKVHFPNLFVDINGISNRMQNRWILDRR 69
Query: 663 QPGLRQILGKERVLIWALVSPN 728
Q LRQI KE VLIWALVSPN
Sbjct: 68 QQCLRQIFWKEWVLIWALVSPN 3
Score = 207 bits (447), Expect = 5e-053
Identities = 96/143 (67%), Positives = 107/143 (74%)
Frame = -1 / +1
Query: 730 RLGDTRAQMSTLSFPRICLSPGCHLCNTQGFCIPLLILSISTKRFGKCILMNFFQDW*KK 551
RLGDTRAQMST SF +ICL C L + Q FCI LLI ISTKRFGKC LM FQ W*KK
Sbjct: 1 RLGDTRAQMSTHSFQKICLRHCCRLSSIQRFCILLLIPLISTKRFGKCTLMKLFQGW*KK 180
Query: 550 EVMVMLDPVLFVTQPACRHSPEGSTMGSLWQRPSFKSPLKLTRQP**PKTVINS*TFSHM 371
EVM +LDPVLFVT+PACRHS + STMGSLWQRPSFKSPLKLT * +TVIN + S
Sbjct: 181 EVMEILDPVLFVTRPACRHSLKESTMGSLWQRPSFKSPLKLTHLQ*KLRTVIN*CSSSPT 360
Query: 370 KRWSVLSNTGWRPKPRSSGKR*T 302
+R SV SN GW+P+ R +R*T
Sbjct: 361 RRSSVPSNIGWKPRLRYLDRR*T 429
>gb|BE060120.2|BE060120 HVSMEg0011I12f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0011I12f, mRNA sequence
Length = 592
Score = 214 bits (461), Expect(2) = 8e-057
Identities = 88/111 (79%), Positives = 95/111 (85%)
Frame = -3 / +3
Query: 752 ETEKLHAQVGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYFDEL 573
ETEKLHA+VGRYK PDEHPFF +DLPE LPP+QYP VLHPIADSININKEIWKMYFDE+
Sbjct: 171 ETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEV 350
Query: 572 LPRLVKEGSDGNAGSSALCDTTCLQALSRRIHYGKFVAEAKFQESPEAYTP 420
+ RLVKE SDGN+ SALCDTTCLQAL +R KFVA AKFQE PEAYTP
Sbjct: 351 ISRLVKERSDGNS*FSALCDTTCLQALFKRNPLWKFVAXAKFQEVPEAYTP 503
Score = 173 bits (373), Expect = 8e-043
Identities = 82/114 (71%), Positives = 91/114 (79%)
Frame = +1 / -1
Query: 412 IMAGV*ASGDS*NLASATNFP*WILLESACKQVVSQRALDPALPSLPSFTNLGRSSSKYI 591
++ GV*ASG S*NLA ATNF LL+ ACKQVVSQRAL+ PSL SFTNL +SSKYI
Sbjct: 511 LLQGV*ASGTS*NLAXATNFHSGFLLKRACKQVVSQRALNQEFPSLLSFTNLEITSSKYI 332
Query: 592 FQISLLILIESAMGCKTLGYCIGGNRGSGRSLEKKGCSSGPLYLPTCAWSFSVS 753
FQISLLIL+ESA+GC+T+GY IGGN SGRS K GCSSG LYLPT A SFSVS
Sbjct: 331 FQISLLILMESAIGCRTVGYWIGGNSVSGRSSGKNGCSSGLLYLPTFACSFSVS 170
Score = 164 bits (353), Expect = 5e-040
Identities = 73/109 (66%), Positives = 82/109 (75%)
Frame = +2 / -2
Query: 425 CKLQGTLETWPLPQTSHSGSFWRVPASRLCHKEHWIQHYHHFLLSPILEEVHQNTFSKSL 604
CKLQG LETWP PQTS F R PASR CHKEH I++ HHF LSP L+ +HQ+TFSKSL
Sbjct: 498 CKLQGPLETWPXPQTSIVDFF*RGPASRSCHKEH*IKNSHHFFLSPTLK*LHQSTFSKSL 319
Query: 605 C*Y**NQQWDAKPLGIA*VATGAQADPWKRKGAHLGPCISQPVHGAFQF 751
C*Y* NQQ DA+PL * AT +QAD +R GAHLG CISQP+ AFQF
Sbjct: 318 C*Y*WNQQ*DAEPLDTG*AATVSQADLLERMGAHLGSCISQPLRVAFQF 172
Score = 160 bits (345), Expect = 6e-039
Identities = 74/109 (67%), Positives = 79/109 (72%)
Frame = -1 / +1
Query: 751 KLKSSMHRLGDTRAQMSTLSFPRICLSPGCHLCNTQGFCIPLLILSISTKRFGKCILMNF 572
KLKS RLGDTRAQMST SF +ICL C L + Q FCI LLI ISTKRFGKC LM
Sbjct: 172 KLKSYTQRLGDTRAQMSTHSFQKICLRHCCRLSSIQRFCILLLIPLISTKRFGKCTLMKL 351
Query: 571 FQDW*KKEVMVMLDPVLFVTQPACRHSPEGSTMGSLWQRPSFKSPLKLT 425
FQ W*KKEVM +LD VLFVT+PACR S + GSLW RPSFK LKLT
Sbjct: 352 FQGW*KKEVMGILDSVLFVTRPACRPSLKEIHYGSLWXRPSFKRSLKLT 498
Score = 148 bits (318), Expect = 3e-035
Identities = 72/109 (66%), Positives = 80/109 (73%)
Frame = -2 / +2
Query: 750 N*KAPCTGWEIQGPR*APFLFQGSA*APVATYAIPKGFASHC*FYQYQQRDLENVF**TS 571
N*KA GWEIQ PR*AP L + SA* VA Y + G AS+C*F+ YQQRDLENV **+
Sbjct: 173 N*KATRKGWEIQEPR*APILSRRSA*DTVAAYPVSNGSASYC*FH*YQQRDLENVL**SY 352
Query: 570 SKIGERRK*W*CWIQCSL*HNLLAGTLQKDPLWEVCGRGQVSRVP*SLH 424
K+GER+K*W IQCSL*H+LLAG L K EVCG GQVSR P*SLH
Sbjct: 353 FKVGERKK*WEFLIQCSL*HDLLAGPL*KKSTMEVCGXGQVSRGP*SLH 499
Score = 148 bits (317), Expect = 4e-035
Identities = 69/109 (63%), Positives = 73/109 (66%)
Frame = +3 / -3
Query: 426 VSFRGLLKLGLCHKLPIVDPSGECLQAGCVXXXXXXXXXXXXFFHQSWKKFIKIHFPNLF 605
VSFR LLKLGLCHKLP E LQAG V FFHQ FIK+HFPNLF
Sbjct: 497 VSFRDLLKLGLCHKLP*WISFKEGLQAGRVTKSTESRIPITSFFHQP*NNFIKVHFPNLF 318
Query: 606 VDIDRISNGMQNPWVLHRWQPGLRQILGKERVLIWALVSPNLCMELFSF 752
VDI+ ISN MQN W+L R Q LRQI KE VLIWALVSPNLC+ LFSF
Sbjct: 317 VDINGISNRMQNRWILDRRQQCLRQIFWKEWVLIWALVSPNLCV*LFSF 171
Score = 26.7 bits (52), Expect(2) = 8e-057
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -1 / +2
Query: 406 KTVINS*TFSHMKRWSVLSNTGWRP 332
+TVIN +R SV SN GW+P
Sbjct: 518 RTVIN*CXLLPTRRSSVPSNIGWKP 592
>gb|BG415849.1|BG415849 HVSMEk0008C14f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0008C14f, mRNA sequence
Length = 660
Score = 194 bits (418), Expect(2) = 3e-053
Identities = 78/126 (61%), Positives = 95/126 (75%)
Frame = -3 / +1
Query: 752 ETEKLHAQVGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYFDEL 573
E E LH +VGRYK P EHPFF +DLP+ PP+Q+ VLHPIADS+NIN++IWKM +DE+
Sbjct: 190 EPETLHPRVGRYKSPYEHPFFPEDLPDTLWPPIQHTAVLHPIADSLNINRQIWKMSYDEV 369
Query: 572 LPRLVKEGSDGNAGSSALCDTTCLQALSRRIHYGKFVAEAKFQESPEAYTPAIIAQDRDQ 393
+PRL KEGS+GN GSSALCDTTCLQ L + I YG A+A E P+AYTP I +D +Q
Sbjct: 370 IPRLAKEGSEGNGGSSALCDTTCLQVLYKTIPYGTSAAQANS*EPPDAYTPGIKPEDGEQ 549
Query: 392 LMNLLT 375
LM L T
Sbjct: 550 LMQLPT 567
Score = 160 bits (344), Expect(2) = 3e-043
Identities = 73/128 (57%), Positives = 85/128 (66%)
Frame = +2 / -3
Query: 365 PFHM*EGS*VDHGLGLLWLACKLQGTLETWPLPQTSHSGSFWRVPASRLCHKEHWIQHYH 544
PF * + V H +L CK QG L+ WP+PQTSH G F+R PASR C +HWI H+
Sbjct: 577 PFVR*GVALVAHRPQVLSQVCKHQGALKNWPVPQTSHRGLFYRGPASRWCRTKHWIHHFP 398
Query: 545 HFLLSPILEEVHQNTFSKSLC*Y**NQQWDAKPLGIA*VATGAQADPWKRKGAHLGPCIS 724
HFLLSP LE +H TFSKS C*Y* NQQ DA+PL * AT QAD +R GAH+G CIS
Sbjct: 397 HFLLSPTLE*LHHRTFSKSAC*Y*GNQQ*DAEPLYAG*AATVYQADLLERMGAHMGSCIS 218
Query: 725 QPVHGAFQ 748
P+ AFQ
Sbjct: 217 PPLGVAFQ 194
Score = 139 bits (298), Expect(2) = 1e-036
Identities = 73/122 (59%), Positives = 82/122 (67%)
Frame = +1 / -2
Query: 388 MS*SRSWAIMAGV*ASGDS*NLASATNFP*WILLESACKQVVSQRALDPALPSLPSFTNL 567
+S S S + GV*ASG S* LA A + P* I+L CKQVVS +ALDP PSLPSF NL
Sbjct: 554 ISCSPSSGFIPGV*ASGGS*ELACAADVP*GIVL*RTCKQVVSHKALDPPFPSLPSFANL 375
Query: 568 GRSSSKYIFQISLLILIESAMGCKTLGYCIGGNRGSGRSLEKKGCSSGPLYLPTCAWSFS 747
G +SS IFQI LLIL ESA+GC+T IGG+ SGRS K GCS G LYLPT S S
Sbjct: 374 GITSS*DIFQICLLILRESAIGCRTAVCWIGGHSVSGRSSGKNGCSYGLLYLPTLGCSVS 195
Query: 748 VS 753
S
Sbjct: 194 GS 189
Score = 128 bits (274), Expect = 4e-029
Identities = 66/119 (55%), Positives = 72/119 (60%)
Frame = -1 / +2
Query: 748 LKSSMHRLGDTRAQMSTLSFPRICLSPGCHLCNTQGFCIPLLILSISTKRFGKCILMNFF 569
LK GDTRA MST SF +ICL L + Q FCI LLI IST RFGKC +M F
Sbjct: 194 LKRYTQGWGDTRAHMSTHSFQKICLIHCGRLSSIQRFCILLLIPLISTGRFGKCPMMKLF 373
Query: 568 QDW*KKEVMVMLDPVLFVTQPACRHSPEGSTMGSLWQRPSFKSPLKLTRQP**PKTVIN 392
Q W KKEV M+DPVL T PACR S + S MG L RP KSPL LT * +TV N
Sbjct: 374 QGWRKKEVREMVDPVLCATPPACRSSIKQSPMGRLRHRPILKSPLMLTHLG*NLRTVSN 550
Score = 126 bits (270), Expect = 1e-028
Identities = 62/102 (60%), Positives = 70/102 (68%)
Frame = -2 / +3
Query: 729 GWEIQGPR*APFLFQGSA*APVATYAIPKGFASHC*FYQYQQRDLENVF**TSSKIGERR 550
G EIQ P *AP L + SA* VA Y G AS+C*F YQQ DLENV **+ SK+GERR
Sbjct: 213 GGEIQEPI*APILSRRSA*YTVAAYPAYSGSASYC*FP*YQQADLENVL**SYSKVGERR 392
Query: 549 K*W*CWIQCSL*HNLLAGTLQKDPLWEVCGRGQVSRVP*SLH 424
K* WIQC + H+LLAG L +PLW+VCG GQ R P* LH
Sbjct: 393 K*GKWWIQCFVRHHLLAGPL*NNPLWDVCGTGQFLRAP*CLH 518
Score = 126 bits (269), Expect = 2e-028
Identities = 66/146 (45%), Positives = 81/146 (55%)
Frame = +3 / -1
Query: 315 PEDLGFGLHPVFDSTLHRFICEKVHELITVLGYYGWRVSFRGLLKLGLCHKLPIVDPSGE 494
P+ L +G P + + ++H L+TVL +Y VS RGLL++GLC + PI D E
Sbjct: 627 PKILAWGSTPCLMARCALLLGRELH*LLTVLRFYPRCVSIRGLLRIGLCRRRPIGDCFIE 448
Query: 495 CLQAGCVXXXXXXXXXXXXFFHQSWKKFIKIHFPNLFVDIDRISNGMQNPWVLHRWQPGL 674
LQAG V FF Q W FI HFPNL VDI ISN MQN +L R +
Sbjct: 447 DLQAGGVAQSTGSTISLTSFFRQPWNNFIIGHFPNLPVDIKGISNRMQNRCMLDRRPQCI 268
Query: 675 RQILGKERVLIWALVSPNLCMELFSF 752
RQI KE VLIWALVSP+ + F F
Sbjct: 267 RQIFWKEWVLIWALVSPHPWV*RFRF 190
Score = 35.0 bits (70), Expect(2) = 3e-043
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +3 / -3
Query: 306 HLLPEDLGFGLHPVFDSTLHRFI 374
HLL E++ G HP+FD T+ F+
Sbjct: 637 HLLSENISLGFHPMFDGTVRPFV 569
Score = 34.5 bits (69), Expect(2) = 3e-053
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = -3 / +2
Query: 386 NLLTYETVERAIEHRVEAKAKIFGQEVNI 300
N L + RAI+H VE +A IFGQEV I
Sbjct: 557 NSLPNKRAHRAIKHGVEPQANIFGQEVII 643
Score = 34.1 bits (68), Expect(2) = 1e-036
Identities = 14/20 (70%), Positives = 15/20 (75%)
Frame = +1 / -1
Query: 301 MFTSCPKILALASTLCSIAR 360
M TSCPKILA ST C +AR
Sbjct: 642 MITSCPKILAWGSTPCLMAR 583
>gb|AV917519.1|AV917519 AV917519 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags10e01 3', mRNA sequence
Length = 706
Score = 159 bits (342), Expect(3) = 9e-052
Identities = 68/77 (88%), Positives = 69/77 (89%)
Frame = -3 / -2
Query: 506 CLQALSRRIHYGKFVAEAKFQESPEAYTPAIIAQDRDQLMNLLTYETVERAIEHRVEAKA 327
CLQALS+RIHYGKFVAEAKFQESPEAYTPAI AQD DQLM LLTYETVERAIEHRVE KA
Sbjct: 678 CLQALSKRIHYGKFVAEAKFQESPEAYTPAIKAQDGDQLMQLLTYETVERAIEHRVETKA 499
Query: 326 KIFGQEVNIGVEDNGSP 276
KIFGQEVNIG E G P
Sbjct: 498 KIFGQEVNIGAEAKGMP 448
Score = 134 bits (287), Expect(2) = 6e-044
Identities = 62/77 (80%), Positives = 67/77 (87%)
Frame = +1 / +2
Query: 277 GLPLSSTPMFTSCPKILALASTLCSIARSTVSYVRRFMS*SRSWAIMAGV*ASGDS*NLA 456
G+PL+S PMFTSCP ILAL ST CS+ARSTVS VR +S*S S A +AGV*ASGDS*NLA
Sbjct: 449 GMPLASAPMFTSCPNILALVSTRCSMARSTVS*VRSCIS*SPS*AFIAGV*ASGDS*NLA 628
Query: 457 SATNFP*WILLESACKQ 507
SATNFP*WILLESACKQ
Sbjct: 629 SATNFP*WILLESACKQ 679
Score = 104 bits (222), Expect(3) = 3e-035
Identities = 45/68 (66%), Positives = 51/68 (75%)
Frame = +3 / +1
Query: 303 VHLLPEDLGFGLHPVFDSTLHRFICEKVHELITVLGYYGWRVSFRGLLKLGLCHKLPIVD 482
VHLL + L G HP+FD TL R + E++H LITVL +Y VSFRGLLKLGLCHKLPIVD
Sbjct: 475 VHLLSKYLSLGFHPMFDGTLDRLVGEELH*LITVLSFYCRCVSFRGLLKLGLCHKLPIVD 654
Query: 483 PSGECLQA 506
ECLQA
Sbjct: 655 SFRECLQA 678
Score = 101 bits (215), Expect(3) = 2e-032
Identities = 44/70 (62%), Positives = 51/70 (72%)
Frame = -2 / -1
Query: 507 LLAGTLQKDPLWEVCGRGQVSRVP*SLHASHNSPRP*STHEPSHI*NGGACYRTQGGGQS 328
LLAGTL K+PLWEVCGRGQVSRVP*SLH + S *ST+ H+ +G AC+RT GG Q
Sbjct: 679 LLAGTL*KNPLWEVCGRGQVSRVP*SLHTCNKSSGR*STNAAPHLRDGRACHRTSGGNQG 500
Query: 327 QDLRARGEHR 298
D+ GEHR
Sbjct: 499 *DIWTGGEHR 470
Score = 92.7 bits (196), Expect(2) = 1e-023
Identities = 43/70 (61%), Positives = 47/70 (67%)
Frame = +2 / +3
Query: 302 CSPLARRSWLWPPPCVR*HAPPFHM*EGS*VDHGLGLLWLACKLQGTLETWPLPQTSHSG 481
CSP + S W PP VR HA P * + VDH LL CKLQGTLETWPLPQTSHSG
Sbjct: 474 CSPPVQIS*PWFPPDVRWHARPSRR*GAALVDHRPELLLQVCKLQGTLETWPLPQTSHSG 653
Query: 482 SFWRVPASRL 511
F RVPAS++
Sbjct: 654 FF*RVPASKV 683
Score = 88.1 bits (186), Expect(2) = 1e-023
Identities = 42/68 (61%), Positives = 49/68 (72%)
Frame = -1 / -3
Query: 505 ACRHSPEGSTMGSLWQRPSFKSPLKLTRQP**PKTVINS*TFSHMKRWSVLSNTGWRPKP 326
ACRHS + STMGSLWQRPSFKSPLKLT * +TVIN + S +R SV SN GW+P+
Sbjct: 677 ACRHSLKESTMGSLWQRPSFKSPLKLTHLQ*KLRTVIN*CSSSPTRRSSVPSNIGWKPRL 498
Query: 325 RSSGKR*T 302
R +R*T
Sbjct: 497 RYLDRR*T 474
Score = 63.4 bits (132), Expect(2) = 6e-044
Identities = 30/36 (83%), Positives = 31/36 (86%)
Frame = +1 / +2
Query: 169 QSSLLSKYAI*TSLVSGMIL*LYSSATKLGTILYTG 276
QSSLLSKYA TSLVSGMIL LYS AT+LG ILYTG
Sbjct: 344 QSSLLSKYATWTSLVSGMILLLYSPATRLGLILYTG 451
Score = 57.0 bits (118), Expect(3) = 9e-052
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = -3 / -2
Query: 275 PVYKIVPSLVAELYSYRIMPLTKEVQIAY 189
PVYKI PSLVA LYS RIMPLTK+VQ+AY
Sbjct: 450 PVYKIRPSLVAGLYSNRIMPLTKDVQVAY 364
Score = 53.3 bits (110), Expect(3) = 3e-035
Identities = 24/38 (63%), Positives = 28/38 (73%)
Frame = +3 / +1
Query: 168 SIQPPKQIRDLNLFG*RHDSVAVQLGDQARNDLVHRWA 281
S+QPP Q+ L++ G*RHD VAVQ DQA DLVHR A
Sbjct: 343 SVQPP*QVCYLDVLG*RHDPVAVQSSDQAGPDLVHRHA 456
Score = 52.8 bits (109), Expect(3) = 2e-032
Identities = 21/35 (60%), Positives = 23/35 (65%)
Frame = -1 / -3
Query: 271 CTRSFRAWSPSCTATESCR*PKRFKSRICLGGWIE 167
CTRS AWS CTAT SCR*P+ + C GGW E
Sbjct: 446 CTRSGPAWSLDCTATGSCR*PRTSR*HTC*GGWTE 342
Score = 41.4 bits (84), Expect(2) = 1e-023
Identities = 19/35 (54%), Positives = 24/35 (68%)
Frame = -2 / -1
Query: 270 VQDRSELGRRAVQLQNHAANQRGSNRVFA*EAGLS 166
VQD+++ GR VQ Q+HAANQ + A EAGLS
Sbjct: 445 VQDQAQPGRWTVQQQDHAANQGRPGSILAKEAGLS 341
Score = 36.8 bits (74), Expect(2) = 1e-023
Identities = 18/35 (51%), Positives = 21/35 (60%)
Frame = +2 / +3
Query: 167 LNPAS*ANTRFEPLWLAA*FCSCTARRPSSERSCT 271
L+PAS A+ WLAA* C CT +RP SCT
Sbjct: 342 LSPASLASMLPGRPWLAA*SCCCTVQRPGWA*SCT 446
Score = 28.6 bits (56), Expect(3) = 3e-035
Identities = 10/17 (58%), Positives = 11/17 (64%)
Frame = +2 / +1
Query: 482 SFWRVPASRLCHKEHWI 532
SF +R CHKEHWI
Sbjct: 655 SFRECLQARSCHKEHWI 705
Score = 25.8 bits (50), Expect(3) = 9e-052
Identities = 10/11 (90%), Positives = 10/11 (90%)
Frame = -3 / -1
Query: 533 GSSALCDTTCL 501
GSSALCDTT L
Sbjct: 706 GSSALCDTTLL 674
Score = 23.1 bits (44), Expect(3) = 2e-032
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = -1 / -2
Query: 532 DPVLFVTQP 506
DPVLFVT+P
Sbjct: 705 DPVLFVTRP 679
>gb|BF260956.1|BF260956 HVSMEf0023G04f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023G04f, mRNA sequence
Length = 746
Score = 193 bits (416), Expect = 1e-048
Identities = 75/86 (87%), Positives = 81/86 (94%)
Frame = -3 / +1
Query: 752 ETEKLHAQVGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYFDEL 573
ETEKLHA+VGRYK PDEHPFF +DLPE LPP+QYP VLHPIADSININKEIWKMYFDE+
Sbjct: 487 ETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTVLHPIADSININKEIWKMYFDEV 666
Query: 572 LPRLVKEGSDGNAGSSALCDTTCLQA 495
+PRLVKEGSDGN+GSSALCDTTCLQA
Sbjct: 667 IPRLVKEGSDGNSGSSALCDTTCLQA 744
Score = 159 bits (341), Expect = 2e-038
Identities = 70/87 (80%), Positives = 75/87 (86%)
Frame = +1 / -1
Query: 493 SACKQVVSQRALDPALPSLPSFTNLGRSSSKYIFQISLLILIESAMGCKTLGYCIGGNRG 672
SACKQVVSQRALDP PSLPSFTNLG +SSKYIFQISLLIL+ESA+GC+T+GY IGGN
Sbjct: 746 SACKQVVSQRALDPEFPSLPSFTNLGITSSKYIFQISLLILMESAIGCRTVGYWIGGNSV 567
Query: 673 SGRSLEKKGCSSGPLYLPTCAWSFSVS 753
SGRS K GCSSG LYLPT A SFSVS
Sbjct: 566 SGRSSGKNGCSSGLLYLPTFACSFSVS 486
Score = 146 bits (313), Expect = 2e-034
Identities = 62/86 (72%), Positives = 70/86 (81%)
Frame = +2 / -2
Query: 494 VPASRLCHKEHWIQHYHHFLLSPILEEVHQNTFSKSLC*Y**NQQWDAKPLGIA*VATGA 673
VPASR CHKEHWIQ++HHFLLSP LE +HQ+TFSKSLC*Y* NQQ DA+PL * AT +
Sbjct: 745 VPASRSCHKEHWIQNFHHFLLSPTLE*LHQSTFSKSLC*Y*WNQQ*DAEPLDTG*AATVS 566
Query: 674 QADPWKRKGAHLGPCISQPVHGAFQF 751
QAD +R GAHLG CISQP+ AFQF
Sbjct: 565 QADLLERMGAHLGSCISQPLRVAFQF 488
Score = 141 bits (302), Expect = 5e-033
Identities = 62/86 (72%), Positives = 66/86 (76%)
Frame = -1 / +2
Query: 751 KLKSSMHRLGDTRAQMSTLSFPRICLSPGCHLCNTQGFCIPLLILSISTKRFGKCILMNF 572
KLKS RLGDTRAQMST SF +ICL C L + Q FCI LLI ISTKRFGKC LM
Sbjct: 488 KLKSYTQRLGDTRAQMSTHSFQKICLRHCCRLSSIQRFCILLLIPLISTKRFGKCTLMKL 667
Query: 571 FQDW*KKEVMVMLDPVLFVTQPACRH 494
FQ W*KKEVM +LDPVLFVT+PACRH
Sbjct: 668 FQGW*KKEVMEILDPVLFVTRPACRH 745
Score = 130 bits (278), Expect = 1e-029
Identities = 55/86 (63%), Positives = 59/86 (68%)
Frame = +3 / -3
Query: 495 CLQAGCVXXXXXXXXXXXXFFHQSWKKFIKIHFPNLFVDIDRISNGMQNPWVLHRWQPGL 674
CLQAG V FFHQ W FIK+HFPNLFVDI+ ISN MQN W+L R Q L
Sbjct: 744 CLQAGRVTKSTGSRISITSFFHQPWNNFIKVHFPNLFVDINGISNRMQNRWILDRRQQCL 565
Query: 675 RQILGKERVLIWALVSPNLCMELFSF 752
RQI KE VLIWALVSPNLC+ LFSF
Sbjct: 564 RQIFWKEWVLIWALVSPNLCV*LFSF 487
Score = 129 bits (277), Expect = 1e-029
Identities = 60/86 (69%), Positives = 67/86 (77%)
Frame = -2 / +3
Query: 750 N*KAPCTGWEIQGPR*APFLFQGSA*APVATYAIPKGFASHC*FYQYQQRDLENVF**TS 571
N*KA GWEIQ PR*AP L + SA* VA Y + G AS+C*F+ YQQRDLENV **+
Sbjct: 489 N*KATRKGWEIQEPR*APILSRRSA*DTVAAYPVSNGSASYC*FH*YQQRDLENVL**SY 668
Query: 570 SKIGERRK*W*CWIQCSL*HNLLAGT 493
SK+GERRK*W WIQCSL*H+LLAGT
Sbjct: 669 SKVGERRK*WKFWIQCSL*HDLLAGT 746
>gb|BG309831.1|BG309831 HVSMEc0013A09f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0013A09f, mRNA sequence
Length = 792
Score = 160 bits (345), Expect = 6e-039
Identities = 64/121 (52%), Positives = 83/121 (68%)
Frame = -3 / +2
Query: 662 PPMQYPRVLHPIADSININKEIWKMYFDELLPRLVKEGSDGNAGSSALCDTTCLQALSRR 483
P YP+VLHP A S+ +N IWKMYF+ELLP +G DGN + D CLQALSRR
Sbjct: 293 PTKGYPKVLHPFASSVCVNDAIWKMYFNELLPLFTADGDDGNYAETVALDFACLQALSRR 472
Query: 482 IHYGKFVAEAKFQESPEAYTPAIIAQDRDQLMNLLTYETVERAIEHRVEAKAKIFGQEVN 303
IH GK+VAE KF+++P Y+P I A+D + LMNLLT+ VE +++RVE A +FGQ V
Sbjct: 473 IHCGKYVAEVKFRDAPHDYSPPIRAKDNNALMNLLTFTGVEEKVKNRVEKTATVFGQNVT 652
Query: 302 I 300
+
Sbjct: 653 L 655
>gb|BF631171.2|BF631171 HVSMEb0015C20f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0015C20f, mRNA sequence
Length = 777
Score = 125 bits (267), Expect = 3e-028
Identities = 48/57 (84%), Positives = 51/57 (89%)
Frame = -3 / +2
Query: 752 ETEKLHAQVGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYF 582
ETEKLHA+VGRYK PDEHPFF +DLPE LPP+QYP LHPIADSININKEIWKMYF
Sbjct: 506 ETEKLHAKVGRYKSPDEHPFFPEDLPETLLPPIQYPTGLHPIADSININKEIWKMYF 676
>gb|BU995988.1|BU995988 HM12A03r HM Hordeum vulgare subsp. vulgare cDNA clone HM12A03
5-PRIME, mRNA sequence
Length = 589
Score = 84.5 bits (178), Expect(2) = 4e-027
Identities = 36/43 (83%), Positives = 36/43 (83%)
Frame = -3 / +3
Query: 404 DRDQLMNLLTYETVERAIEHRVEAKAKIFGQEVNIGVEDNGSP 276
D DQLM LLTYETVERAIEHRVE KAKIFGQEVNIG E G P
Sbjct: 9 DGDQLMQLLTYETVERAIEHRVETKAKIFGQEVNIGAEAKGMP 137
Score = 68.9 bits (144), Expect(2) = 2e-024
Identities = 31/43 (72%), Positives = 35/43 (81%)
Frame = +1 / -1
Query: 277 GLPLSSTPMFTSCPKILALASTLCSIARSTVSYVRRFMS*SRS 405
G+PL+S PMFTSCP ILAL ST CS+ARSTVS VR +S*S S
Sbjct: 136 GMPLASAPMFTSCPNILALVSTRCSMARSTVS*VRSCIS*SPS 8
Score = 63.4 bits (132), Expect(2) = 2e-024
Identities = 30/36 (83%), Positives = 31/36 (86%)
Frame = +1 / -1
Query: 169 QSSLLSKYAI*TSLVSGMIL*LYSSATKLGTILYTG 276
QSSLLSKYA TSLVSGMIL LYS AT+LG ILYTG
Sbjct: 241 QSSLLSKYATWTSLVSGMILLLYSPATRLGLILYTG 134
Score = 57.0 bits (118), Expect(2) = 4e-027
Identities = 24/29 (82%), Positives = 26/29 (89%)
Frame = -3 / +3
Query: 275 PVYKIVPSLVAELYSYRIMPLTKEVQIAY 189
PVYKI PSLVA LYS RIMPLTK+VQ+AY
Sbjct: 135 PVYKIRPSLVAGLYSNRIMPLTKDVQVAY 221
>gb|BF623091.2|BF623091 HVSMEa0014D14f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0014D14f, mRNA sequence
Length = 903
Score = 106 bits (226), Expect = 2e-022
Identities = 44/106 (41%), Positives = 58/106 (54%)
Frame = -3 / +2
Query: 752 ETEKLHAQVGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYFDEL 573
E + LHA+ G Y+ P++ PF S DLP P P YP+VLHP A S N WKMY +EL
Sbjct: 176 EAQVLHAKAGHYQKPEDVPFLSHDLPPPVFPTKGYPKVLHPSATSACENDAFWKMYLNEL 355
Query: 572 LPRLVKEGSDGNAGSSALCDTTCLQALSRRIHYGKFVAEAKFQESP 435
LP + +GN +A D C++ LSR K KF ++P
Sbjct: 356 LPLFTADWHEGNYAKTAALDFACMKLLSREFIVVKMPLRVKFNDAP 493
>gb|BE455736.3|BE455736 HVSMEg0019G20f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0019G20f, mRNA sequence
Length = 829
Score = 75.3 bits (158), Expect(3) = 2e-021
Identities = 31/57 (54%), Positives = 39/57 (68%)
Frame = -3 / +2
Query: 752 ETEKLHAQVGRYKGPDEHPFFSKDLPEPRLPPMQYPRVLHPIADSININKEIWKMYF 582
+TEKLHA+ GRYK P+E PFFSK LPE + P+ Y L DSIN N++ W+ YF
Sbjct: 488 KTEKLHAKGGRYKSPNEAPFFSKKLPETLVAPIPYSTGLDSNGDSINNNQKDWEKYF 658
Score = 35.0 bits (70), Expect(3) = 2e-021
Identities = 16/30 (53%), Positives = 18/30 (60%)
Frame = -1 / +1
Query: 604 KRFGKCILMNFFQDW*KKEVMVMLDPVLFV 515
KR GK + M FQ W*KKE L P +FV
Sbjct: 637 KRLGKVLWMKVFQGW*KKEGNENLGPGVFV 726
Score = 29.5 bits (58), Expect(3) = 2e-021
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = -2 / +1
Query: 504 LAGTLQKDPLWEVCGRGQVSRVP*SLH 424
L ++ P WEVC + Q + P LH
Sbjct: 739 LGDPFKRKPFWEVCAKAQF*KAPRRLH 819
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 450,650,432
Number of Sequences: 312970
Number of extensions: 9270967
Number of successful extensions: 115677
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8525758509
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)