TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2521353.2.1
(1279 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BQ765051.1|BQ765051 EBca01_SQ005_L18_R carpel, pre-anthe... 352 e-126
gb|BQ762868.1|BQ762868 EBro02_SQ006_I12_R root, 3 week, hyd... 393 e-122
gb|CA007593.1|CA007593 HU08H01r HU Hordeum vulgare subsp. v... 155 2e-069
gb|BQ764116.1|BQ764116 EBro03_SQ006_G08_R root, 3 week, wat... 224 9e-062
gb|BM373085.2|BM373085 EBma04_SQ003_A16_R maternal, 10 DPA,... 224 9e-062
gb|BU987907.1|BU987907 HF16B16r HF Hordeum vulgare subsp. v... 214 7e-059
gb|BU987948.1|BU987948 HF16D20r HF Hordeum vulgare subsp. v... 214 7e-059
gb|CA005867.1|CA005867 HU08H01u HU Hordeum vulgare subsp. v... 139 2e-036
gb|BE216718.2|BE216718 HV_CEb0011F09f Hordeum vulgare seedl... 137 2e-031
gb|CA007353.1|CA007353 HU07K19r HU Hordeum vulgare subsp. v... 128 7e-029
gb|BG368245.2|BG368245 HVSMEi0017B05f Hordeum vulgare 20 DA... 124 8e-028
gb|AJ462714.1|AJ462714 AJ462714 S00002 Hordeum vulgare subs... 106 9e-027
gb|BU999143.1|BU999143 HI13G13r HI Hordeum vulgare subsp. v... 114 9e-025
gb|BE215201.1|BE215201 HV_CEb0006C07f Hordeum vulgare seedl... 113 2e-024
gb|BF255677.2|BF255677 HVSMEf0007I02f Hordeum vulgare seedl... 106 2e-022
gb|CA027915.1|CA027915 HZ60G23r HZ Hordeum vulgare subsp. v... 104 1e-021
gb|CA028055.1|CA028055 HZ60N16r HZ Hordeum vulgare subsp. v... 104 1e-021
gb|CK124312.1|CK124312 BES1824108f09 BES1824 Hordeum vulgar... 89 2e-021
>gb|BQ765051.1|BQ765051 EBca01_SQ005_L18_R carpel, pre-anthesis, no treatment, cv Optic,
EBca01 Hordeum vulgare subsp. vulgare cDNA clone
EBca01_SQ005_L18 5', mRNA sequence
Length = 700
Score = 352 bits (763), Expect(2) = e-126
Identities = 142/149 (95%), Positives = 148/149 (99%)
Frame = -3 / +3
Query: 1199 GLGTLRAVLAILQWWGFNVTVIIMNKWIFQKLDFKFPLTVSCVHFICSSIGAYIAIHVLK 1020
GLG++RAVLAILQWWGFNVTVII+NKWIFQKLDFKFPLTVSCVHFICSSIGAY+AIHVLK
Sbjct: 105 GLGSMRAVLAILQWWGFNVTVIIINKWIFQKLDFKFPLTVSCVHFICSSIGAYVAIHVLK 284
Query: 1019 AKPLIQVEPEDRWKRIFPMSFVFCINIVLGNVSLRYIPVSFMQTIKSFTPATTVILQWLV 840
AKPLIQVEPEDRWKRIFPMSFVFC+NIVL NVSLRYIPVSFMQTIKSFTPATTVILQWLV
Sbjct: 285 AKPLIQVEPEDRWKRIFPMSFVFCMNIVLRNVSLRYIPVSFMQTIKSFTPATTVILQWLV 464
Query: 839 WSKHFEWRIWASLVPIVGGILLTSVTELS 753
WSKHFEWRIWASLVPIVGGILLTS+TELS
Sbjct: 465 WSKHFEWRIWASLVPIVGGILLTSMTELS 551
Score = 314 bits (681), Expect(2) = e-110
Identities = 131/153 (85%), Positives = 139/153 (90%)
Frame = +1 / -1
Query: 742 KMLKLSSVTEVRSIPPTIGTSEAHMRHSKCLLQTSHCRITVVAGVKDLIVCMKETGM*RK 921
K +KLSSV EVR IPPTIGTSEAHMRHSKCLLQT+HCRITVVAGV DL+VCMKETGM*R+
Sbjct: 562 KNVKLSSVIEVRRIPPTIGTSEAHMRHSKCLLQTNHCRITVVAGVNDLMVCMKETGM*RR 383
Query: 922 LTFPSTMFMQKTKDIGNILFQRSSGST*IRGFALRTWIAM*APMEEQMKWTQDTVRGNLK 1101
LTF STMFMQ TKD+G ILFQRSSGST*I GFAL TWIA *AP+EE MKWTQDTV+GNLK
Sbjct: 382 LTFLSTMFMQNTKDMGKILFQRSSGST*INGFALSTWIAT*APIEEHMKWTQDTVKGNLK 203
Query: 1102 SSFWKIHLFMMMTVTLKPHHWRMASTARRVPRP 1200
SSFWKIHL MMMTVTLKPHHWRMASTAR +P P
Sbjct: 202 SSFWKIHLLMMMTVTLKPHHWRMASTARMLPSP 104
Score = 222 bits (480), Expect(2) = 4e-078
Identities = 97/141 (68%), Positives = 110/141 (78%)
Frame = +3 / -3
Query: 756 KLCHRSQEYSANYRDQRSPYAPLEMLAPD*PLQNNCGCRSERFDCLHEGDWDVAQADISQ 935
KL H QE +YRDQRSPYAPL++LAP+ PLQNNCGCR ERF+ LHEGD DVAQA+IS
Sbjct: 548 KLRH*GQEDPPDYRDQRSPYAPLKVLAPNQPLQNNCGCRGERFNGLHEGDRDVAQANIS* 369
Query: 936 HNVYAEDEGHWKYPFPAVLRFNLNQGFRLEDMDCNVSSNGRADEVDAGHGERELEIQFLE 1115
HNV+AE EGH + P PAVL +LNQ LE +D +VS + A EVDAGH + ELEIQ LE
Sbjct: 368 HNVHAEHEGHGEDPLPAVLGLDLNQWLCLEHVDRDVSPDRGAYEVDAGHRQGELEIQLLE 189
Query: 1116 DPLVHDDDSDVEAPPLEDGEH 1178
DPLV DDD DVEAPPLEDGEH
Sbjct: 188 DPLVDDDDGDVEAPPLEDGEH 126
Score = 205 bits (443), Expect(2) = 1e-075
Identities = 89/142 (62%), Positives = 101/142 (71%)
Frame = -2 / +2
Query: 1161 VVGLQRHCHHHEQVDLPETGFQVPSHRVLRPLHLLFHWSLHCNPCPQGETPDSS*TGGPL 982
VVGLQRH HHH+QVDLPE GFQVP VLRPLH+L + L +P QG+ DS GPL
Sbjct: 143 VVGLQRHRHHHQQVDLPEAGFQVPLDGVLRPLHMLLYRGLRRDPRAQGKAIDSGRARGPL 322
Query: 981 EKDISNVLRLLHKHCAGKCQLALHPSLLHADNQIFHSCNHSYSAVASLEQAFRVAHMGFA 802
E+D+ +VLR+LH+HCA KC ALHP LLHAD+ I H CNHSYSAV LEQA VAHMGFA
Sbjct: 323 EEDLPHVLRVLHEHCAKKC*PALHPGLLHADH*IVHPCNHSYSAVVGLEQAL*VAHMGFA 502
Query: 801 GPYSWRNTPDFGDRA*LQHFRF 736
GP S + PD D A* F F
Sbjct: 503 GPDSRGDPPDLNDGA*FNIFGF 568
Score = 119 bits (255), Expect(2) = e-126
Identities = 48/50 (96%), Positives = 50/50 (100%)
Frame = -3 / +2
Query: 752 FNIFGFCAAMVGCLATSTKTILAESLLHGYKFDSINTVYYMAPFATMILA 603
FNIFGFCAAM+GCLATSTKTILAESLLHGYKFDSINT+YYMAPFATMILA
Sbjct: 551 FNIFGFCAAMIGCLATSTKTILAESLLHGYKFDSINTLYYMAPFATMILA 700
Score = 115 bits (245), Expect(4) = 1e-045
Identities = 60/98 (61%), Positives = 68/98 (69%)
Frame = +2 / -2
Query: 752 S*ALSPKSGVFRQL*GPAKPICATRNACSRLATAE*LWLQE*KI*LSA*RRLGCSAS*HF 931
+*A S +SG +L GPAKPICAT++ACS+ TAE*LWLQ * I* SA*RR GCSA *HF
Sbjct: 552 N*APSLRSGGSPRLSGPAKPICATQSACSKPTTAE*LWLQG*TI*WSA*RRPGCSAG*HF 373
Query: 932 PAQCLCRRRRTLEISFSSGPPVQLESGVSP*GHGLQCK 1045
AQC CR RRT S SSGP + ES P* G + K
Sbjct: 372 LAQCSCRTRRTWGRSSSSGPRARPESMALP*ARGSRRK 259
Score = 105 bits (224), Expect(2) = e-110
Identities = 46/49 (93%), Positives = 46/49 (93%)
Frame = +1 / -2
Query: 604 ASIMVAKGAM*YTVLMLSNLYPWSRDSAKMVFVDVARQPTMAAQKPKML 750
ASIMVAKGAM*Y VLMLSNLYPWSRDSAKMV VDVARQP MAAQKPKML
Sbjct: 699 ASIMVAKGAM*YNVLMLSNLYPWSRDSAKMVLVDVARQPIMAAQKPKML 553
Score = 102 bits (217), Expect(2) = 4e-040
Identities = 53/97 (54%), Positives = 62/97 (63%)
Frame = -1 / +1
Query: 1045 LTLQSMSSRRNP*FKLNRRTAGKGYFQCPSSSA*TLCWEMSACATSQSPSCRQSNLSLLQ 866
LT +S SR++ *F+ + RTAG+G CPS SA*TLC EM ACATS+SPSCR N S LQ
Sbjct: 259 LTSRSTCSRQSH*FRSSPRTAGRGSSPCPSCSA*TLC*EMLACATSRSPSCRPLNRSPLQ 438
Query: 865 PQLFCXXXXXXXXXXXXXGLRWSL*LAEYS*LR*QSL 755
PQLFC GLRWS * S* +*+SL
Sbjct: 439 PQLFCSGWFGASTLSGAYGLRWSR*SGGSS*PQ*RSL 549
Score = 98.7 bits (209), Expect(2) = 1e-075
Identities = 36/48 (75%), Positives = 40/48 (83%)
Frame = -2 / +1
Query: 747 HFRFLCCHGWLPGYVYKDHLGRVPTPWIQV*QH*HGVLHGTLCHHDTG 604
HF FLCCH LP +VY+DHLGRVP P IQ+*QH*H +LHGT CHHDTG
Sbjct: 556 HFWFLCCHDRLPRHVYQDHLGRVPAPRIQI*QH*HIILHGTFCHHDTG 699
Score = 90.0 bits (190), Expect(2) = 4e-078
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = +3 / -1
Query: 603 SQYHGGKGCHVVHRVNAVKLVSME*GLCQDGLCRRSQAANHGSTETENVE 752
SQYHGGK CHVV VNAVK VS+E GLCQDGL RR +AA HGSTET+NV+
Sbjct: 700 SQYHGGKRCHVV*CVNAVKFVSVEQGLCQDGLGRRGEAAYHGSTETKNVK 551
Score = 83.5 bits (176), Expect(2) = 4e-040
Identities = 37/51 (72%), Positives = 39/51 (76%)
Frame = -1 / +3
Query: 757 LASTFSVSVLPWLAAWLRLQRPSWQSPYSMDTSLTALTRCTTWHPLPP*YW 605
L+ TF VSVLP AA RL RPSWQSP S DT+LTALT TTWH LPP*YW
Sbjct: 546 LSLTFLVSVLP**AASPRLPRPSWQSPCSTDTNLTALTHYTTWHLLPP*YW 698
Score = 52.8 bits (109), Expect(4) = 1e-045
Identities = 21/30 (70%), Positives = 23/30 (76%)
Frame = +2 / -3
Query: 665 IHGVGTLPRWSL*T*PGSQPWQHRNRKC*S 754
I G GTLPRWS *T GS WQHRN+KC*+
Sbjct: 638 IRGAGTLPRWSW*TWRGSLSWQHRNQKC*T 549
Score = 47.8 bits (98), Expect(4) = 1e-045
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +2 / -2
Query: 1097 *NPVSGRSTCS***Q*R*SPTTGGW 1171
*NP SGRSTC ***+*R*SPTTGGW
Sbjct: 207 *NPASGRSTC****R*R*SPTTGGW 133
Score = 24.9 bits (48), Expect(4) = 1e-045
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = +2 / -3
Query: 605 PVSWWQRV 628
PVSWWQ+V
Sbjct: 698 PVSWWQKV 675
>gb|BQ762868.1|BQ762868 EBro02_SQ006_I12_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ006_I12 5', mRNA sequence
Length = 699
Score = 393 bits (852), Expect = e-108
Identities = 167/199 (83%), Positives = 175/199 (87%)
Frame = +1 / -2
Query: 604 ASIMVAKGAM*YTVLMLSNLYPWSRDSAKMVFVDVARQPTMAAQKPKMLKLSSVTEVRSI 783
ASIMVAKGAM*YTVLMLSNLYPWSRDSAKMV VDVARQP MAAQKPKML +
Sbjct: 698 ASIMVAKGAM*YTVLMLSNLYPWSRDSAKMVLVDVARQPIMAAQKPKMLN*APSLRSGGS 519
Query: 784 PPTIGTSEAHMRHSKCLLQTSHCRITVVAGVKDLIVCMKETGM*RKLTFPSTMFMQKTKD 963
P IGTSEAHMRHSKCLLQT+HCRITVVAGV DL+VCMKETGM*R+LTFPSTMFMQ TKD
Sbjct: 518 PRXIGTSEAHMRHSKCLLQTNHCRITVVAGVNDLMVCMKETGM*RRLTFPSTMFMQNTKD 339
Query: 964 IGNILFQRSSGST*IRGFALRTWIAM*APMEEQMKWTQDTVRGNLKSSFWKIHLFMMMTV 1143
+G ILFQRSSGST*I GFAL TWIA *AP+EE MKWTQDTV+GNLKSSFWKIHL MMMTV
Sbjct: 338 MGKILFQRSSGST*INGFALSTWIAT*APIEEHMKWTQDTVKGNLKSSFWKIHLLMMMTV 159
Query: 1144 TLKPHHWRMASTARRVPRP 1200
TLKPHHWRMASTAR +P P
Sbjct: 158 TLKPHHWRMASTARMLPSP 102
Score = 331 bits (718), Expect(3) = e-122
Identities = 132/139 (94%), Positives = 137/139 (98%)
Frame = -3 / +1
Query: 1199 GLGTLRAVLAILQWWGFNVTVIIMNKWIFQKLDFKFPLTVSCVHFICSSIGAYIAIHVLK 1020
GLG++RAVLAILQWWGFNVTVII+NKWIFQKLDFKFPLTVSCVHFICSSIGAY+AIHVLK
Sbjct: 103 GLGSMRAVLAILQWWGFNVTVIIINKWIFQKLDFKFPLTVSCVHFICSSIGAYVAIHVLK 282
Query: 1019 AKPLIQVEPEDRWKRIFPMSFVFCINIVLGNVSLRYIPVSFMQTIKSFTPATTVILQWLV 840
AKPLIQVEPEDRWKRIFPMSFVFC+NIVLGNVSLRYIPVSFMQTIKSFTPATTVILQWLV
Sbjct: 283 AKPLIQVEPEDRWKRIFPMSFVFCMNIVLGNVSLRYIPVSFMQTIKSFTPATTVILQWLV 462
Query: 839 WSKHFEWRIWASLVPIVGG 783
WSKHFEWRIWASLVPI G
Sbjct: 463 WSKHFEWRIWASLVPIXRG 519
Score = 299 bits (648), Expect = 2e-080
Identities = 130/192 (67%), Positives = 148/192 (77%)
Frame = +3 / -1
Query: 603 SQYHGGKGCHVVHRVNAVKLVSME*GLCQDGLCRRSQAANHGSTETENVEAKLCHRSQEY 782
SQYHGGK CHVV+ VNAVK VS+E GLCQDGL RR +AA HGSTET+NV+ +
Sbjct: 699 SQYHGGKRCHVVYCVNAVKFVSVEQGLCQDGLGRRGEAAYHGSTETKNVKLSSVIEVRRI 520
Query: 783 SANYRDQRSPYAPLEMLAPD*PLQNNCGCRSERFDCLHEGDWDVAQADISQHNVYAEDEG 962
YRDQRSPYAPL++LAP+ PLQNNCGCR ERF+ LHEGD DVAQA+IS HNV+AE EG
Sbjct: 519 PPXYRDQRSPYAPLKVLAPNQPLQNNCGCRGERFNGLHEGDRDVAQANIS*HNVHAEHEG 340
Query: 963 HWKYPFPAVLRFNLNQGFRLEDMDCNVSSNGRADEVDAGHGERELEIQFLEDPLVHDDDS 1142
H + P PAVL +LNQ LE +D +VS + A EVDAGH + ELEIQ LEDPLV DDD
Sbjct: 339 HGEDPLPAVLGLDLNQWLCLEHVDRDVSPDRGAYEVDAGHRQGELEIQLLEDPLVDDDDG 160
Query: 1143 DVEAPPLEDGEH 1178
DVEAPPLEDGEH
Sbjct: 159 DVEAPPLEDGEH 124
Score = 194 bits (418), Expect(2) = 9e-073
Identities = 81/122 (66%), Positives = 92/122 (75%)
Frame = -2 / +3
Query: 1161 VVGLQRHCHHHEQVDLPETGFQVPSHRVLRPLHLLFHWSLHCNPCPQGETPDSS*TGGPL 982
VVGLQRH HHH+QVDLPE GFQVP VLRPLH+L + L +P QG+ DS GPL
Sbjct: 141 VVGLQRHRHHHQQVDLPEAGFQVPLDGVLRPLHMLLYRGLRRDPRAQGKAIDSGRARGPL 320
Query: 981 EKDISNVLRLLHKHCAGKCQLALHPSLLHADNQIFHSCNHSYSAVASLEQAFRVAHMGFA 802
E+D+ +VLR+LH+HCA KC ALHP LLHAD+ I H CNHSYSAV LEQA VAHMGFA
Sbjct: 321 EEDLPHVLRVLHEHCARKC*PALHPGLLHADH*IVHPCNHSYSAVVGLEQAL*VAHMGFA 500
Query: 801 GP 796
GP
Sbjct: 501 GP 506
Score = 121 bits (258), Expect(3) = e-122
Identities = 49/50 (98%), Positives = 50/50 (100%)
Frame = -3 / +1
Query: 752 FNIFGFCAAMVGCLATSTKTILAESLLHGYKFDSINTVYYMAPFATMILA 603
FNIFGFCAAM+GCLATSTKTILAESLLHGYKFDSINTVYYMAPFATMILA
Sbjct: 550 FNIFGFCAAMIGCLATSTKTILAESLLHGYKFDSINTVYYMAPFATMILA 699
Score = 110 bits (235), Expect(5) = 2e-047
Identities = 55/85 (64%), Positives = 60/85 (70%)
Frame = +2 / -3
Query: 791 L*GPAKPICATRNACSRLATAE*LWLQE*KI*LSA*RRLGCSAS*HFPAQCLCRRRRTLE 970
L GPAKPICAT++ACS+ TAE*LWLQ * I* SA*RR GCSA *HF AQC CR RRT
Sbjct: 511 LSGPAKPICATQSACSKPTTAE*LWLQG*TI*WSA*RRPGCSAG*HFLAQCSCRTRRTWG 332
Query: 971 ISFSSGPPVQLESGVSP*GHGLQCK 1045
S SSGP + ES P* G + K
Sbjct: 331 RSSSSGPRARPESMALP*ARGSRRK 257
Score = 100 bits (213), Expect(2) = 9e-073
Identities = 36/48 (75%), Positives = 40/48 (83%)
Frame = -2 / +3
Query: 747 HFRFLCCHGWLPGYVYKDHLGRVPTPWIQV*QH*HGVLHGTLCHHDTG 604
HF FLCCH LP +VY+DHLGRVP P IQ+*QH*H +LHGT CHHDTG
Sbjct: 555 HFWFLCCHDRLPRHVYQDHLGRVPAPRIQI*QH*HSILHGTFCHHDTG 698
Score = 99.6 bits (211), Expect(2) = 2e-039
Identities = 47/83 (56%), Positives = 54/83 (65%)
Frame = -1 / +2
Query: 1045 LTLQSMSSRRNP*FKLNRRTAGKGYFQCPSSSA*TLCWEMSACATSQSPSCRQSNLSLLQ 866
LT +S SR++ *F+ + RTAG+G CPS SA*TLC EM ACATS+SPSCR N S LQ
Sbjct: 257 LTSRSTCSRQSH*FRSSPRTAGRGSSPCPSCSA*TLC*EMLACATSRSPSCRPLNRSPLQ 436
Query: 865 PQLFCXXXXXXXXXXXXXGLRWS 797
PQLFC GLRWS
Sbjct: 437 PQLFCSGWFGASTLSGAYGLRWS 505
Score = 84.0 bits (177), Expect(2) = 2e-039
Identities = 37/51 (72%), Positives = 40/51 (78%)
Frame = -1 / +2
Query: 757 LASTFSVSVLPWLAAWLRLQRPSWQSPYSMDTSLTALTRCTTWHPLPP*YW 605
L+ TF VSVLP AA RL RPSWQSP S DT+LTALT+ TTWH LPP*YW
Sbjct: 545 LSLTFLVSVLP**AASPRLPRPSWQSPCSTDTNLTALTQYTTWHLLPP*YW 697
Score = 52.8 bits (109), Expect(5) = 2e-047
Identities = 21/30 (70%), Positives = 23/30 (76%)
Frame = +2 / -3
Query: 665 IHGVGTLPRWSL*T*PGSQPWQHRNRKC*S 754
I G GTLPRWS *T GS WQHRN+KC*+
Sbjct: 637 IRGAGTLPRWSW*TWRGSLSWQHRNQKC*T 548
Score = 47.8 bits (98), Expect(5) = 2e-047
Identities = 22/25 (88%), Positives = 23/25 (92%)
Frame = +2 / -3
Query: 1097 *NPVSGRSTCS***Q*R*SPTTGGW 1171
*NP SGRSTC ***+*R*SPTTGGW
Sbjct: 205 *NPASGRSTC****R*R*SPTTGGW 131
Score = 28.1 bits (55), Expect(5) = 2e-047
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = +1 / -1
Query: 742 KMLKLSSVTEVRSIPP 789
K +KLSSV EVR IPP
Sbjct: 561 KNVKLSSVIEVRRIPP 514
Score = 26.7 bits (52), Expect(3) = e-122
Identities = 11/12 (91%), Positives = 12/12 (100%)
Frame = -3 / +2
Query: 788 GGILLTSVTELS 753
GGILLTS+TELS
Sbjct: 515 GGILLTSMTELS 550
Score = 24.9 bits (48), Expect(5) = 2e-047
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = +2 / -3
Query: 605 PVSWWQRV 628
PVSWWQ+V
Sbjct: 697 PVSWWQKV 674
>gb|CA007593.1|CA007593 HU08H01r HU Hordeum vulgare subsp. vulgare cDNA clone HU08H01
5-PRIME, mRNA sequence
Length = 512
Score = 155 bits (332), Expect(3) = 2e-069
Identities = 64/72 (88%), Positives = 68/72 (94%)
Frame = -3 / +3
Query: 1073 VHFICSSIGAYIAIHVLKAKPLIQVEPEDRWKRIFPMSFVFCINIVLGNVSLRYIPVSFM 894
+H + IGAY+AIHVLKAKPLIQVEPEDRWKRIFPMSFVFC+NIVLGNVSLRYIPVSFM
Sbjct: 261 LHPLHMPIGAYVAIHVLKAKPLIQVEPEDRWKRIFPMSFVFCMNIVLGNVSLRYIPVSFM 440
Query: 893 QTIKSFTPATTV 858
QTIKSFTPATTV
Sbjct: 441 QTIKSFTPATTV 476
Score = 126 bits (269), Expect(3) = 4e-056
Identities = 55/65 (84%), Positives = 57/65 (87%)
Frame = +1 / -2
Query: 859 TVVAGVKDLIVCMKETGM*RKLTFPSTMFMQKTKDIGNILFQRSSGST*IRGFALRTWIA 1038
TVVAGVKDLIVCMKETGM R+LTFPSTMFMQ TKD+G ILFQRSSGST I GFAL TWIA
Sbjct: 475 TVVAGVKDLIVCMKETGMYRRLTFPSTMFMQNTKDMGKILFQRSSGSTCINGFALSTWIA 296
Query: 1039 M*APM 1053
APM
Sbjct: 295 TYAPM 281
Score = 113 bits (242), Expect(3) = 2e-069
Identities = 43/49 (87%), Positives = 47/49 (95%)
Frame = -3 / +1
Query: 1199 GLGTLRAVLAILQWWGFNVTVIIMNKWIFQKLDFKFPLTVSCVHFICSS 1053
GLG++RAVLAILQWWGF+VTVII NKWIFQKLDFKFPLTVSC+HFIC S
Sbjct: 139 GLGSMRAVLAILQWWGFHVTVIINNKWIFQKLDFKFPLTVSCIHFICPS 285
Score = 102 bits (217), Expect(3) = 4e-056
Identities = 38/46 (82%), Positives = 41/46 (89%)
Frame = +1 / -1
Query: 1063 MKWTQDTVRGNLKSSFWKIHLFMMMTVTLKPHHWRMASTARRVPRP 1200
MKW QDTVRGNLKS+FWKIHL +MMTVT KPHHWRMASTAR +P P
Sbjct: 275 MKWMQDTVRGNLKSNFWKIHLLLMMTVTWKPHHWRMASTARMLPSP 138
Score = 95.9 bits (203), Expect(3) = 6e-040
Identities = 39/65 (60%), Positives = 46/65 (70%)
Frame = +3 / -1
Query: 861 CGCRSERFDCLHEGDWDVAQADISQHNVYAEDEGHWKYPFPAVLRFNLNQGFRLEDMDCN 1040
CGCR E FD LHEGD DV QAD+S+HNV+AE EGH + P P VL NL Q RLE +D +
Sbjct: 473 CGCRGEGFDSLHEGDRDVPQADVSEHNVHAEHEGHGEDPLPTVLGLNLYQRLRLEHVDRD 294
Query: 1041 VSSNG 1055
V +G
Sbjct: 293 VCPDG 279
Score = 92.7 bits (196), Expect(3) = 3e-035
Identities = 37/65 (56%), Positives = 46/65 (70%)
Frame = -2 / +2
Query: 1053 HWSLHCNPCPQGETPDSS*TGGPLEKDISNVLRLLHKHCAGKCQLALHPSLLHADNQIFH 874
H + +P QGE D+ * GPLE+D+ +VLR+LH+HCA K Q A+HP LLHAD QI H
Sbjct: 281 HRGIRRDPRAQGEAVDTG*ARGPLEEDLPHVLRVLHEHCARKRQPAVHPGLLHADYQILH 460
Query: 873 SCNHS 859
CNHS
Sbjct: 461 PCNHS 475
Score = 79.4 bits (167), Expect(3) = 6e-040
Identities = 33/40 (82%), Positives = 34/40 (85%)
Frame = +3 / -3
Query: 1059 ADEVDAGHGERELEIQFLEDPLVHDDDSDVEAPPLEDGEH 1178
A EVDAGH + ELEIQ LEDPLV DDD DVEAPPLEDGEH
Sbjct: 279 AYEVDAGHRQGELEIQLLEDPLVVDDDGDVEAPPLEDGEH 160
Score = 62.0 bits (129), Expect(3) = 3e-035
Identities = 24/34 (70%), Positives = 26/34 (76%)
Frame = -2 / +3
Query: 1161 VVGLQRHCHHHEQVDLPETGFQVPSHRVLRPLHL 1060
VVGL RH HH +QVDLPE GFQVP VL PLH+
Sbjct: 177 VVGLPRHRHHQQQVDLPEVGFQVPPDGVLHPLHM 278
Score = 37.7 bits (76), Expect(3) = 2e-069
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = -2 / +2
Query: 645 HGVLHGTLCHHDTG 604
H +LHGT CHHDTG
Sbjct: 470 HSILHGTFCHHDTG 511
Score = 36.8 bits (74), Expect(3) = 3e-035
Identities = 15/19 (78%), Positives = 15/19 (78%)
Frame = -3 / +3
Query: 659 FDSINTVYYMAPFATMILA 603
F TVYYMAPFATMILA
Sbjct: 456 FTPATTVYYMAPFATMILA 512
Score = 33.1 bits (66), Expect(3) = 4e-056
Identities = 11/13 (84%), Positives = 12/13 (92%)
Frame = +3 / -1
Query: 603 SQYHGGKGCHVVH 641
SQYHGGK CHVV+
Sbjct: 512 SQYHGGKRCHVVY 474
Score = 31.8 bits (63), Expect(3) = 6e-040
Identities = 14/15 (93%), Positives = 15/15 (100%)
Frame = +1 / -2
Query: 604 ASIMVAKGAM*YTVL 648
ASIMVAKGAM*YTV+
Sbjct: 511 ASIMVAKGAM*YTVV 467
>gb|BQ764116.1|BQ764116 EBro03_SQ006_G08_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ006_G08 5', mRNA sequence
Length = 718
Score = 224 bits (483), Expect(2) = 9e-062
Identities = 92/105 (87%), Positives = 98/105 (93%)
Frame = -3 / +2
Query: 587 LEGGGVMNWFYTHDSIVPALTIILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVA 408
LEGGGV++WFYTHDS+ +L IIL SGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVA
Sbjct: 2 LEGGGVIDWFYTHDSVFSSLIIILSSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVA 181
Query: 407 VLVSWSIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQRQAAAP 273
VLVSW IFRNPIS MNAIGC ITLVGCTFYGYVRHLISQ++AAAP
Sbjct: 182 VLVSWLIFRNPISPMNAIGCAITLVGCTFYGYVRHLISQQKAAAP 316
Score = 157 bits (338), Expect(2) = 7e-040
Identities = 71/105 (67%), Positives = 76/105 (72%)
Frame = +1 / -2
Query: 274 GAAAWRCEIRCLT*P*KVQPTRVIPHPIAFIAEIGFRKIDHDXXXXXXXXXXXXXXNVTA 453
GAAA+ E+RCLT P*KVQPT VI HPIAFI EIGFRKI H+ VTA
Sbjct: 315 GAAAFCWEMRCLTYP*KVQPTSVIAHPIAFIGEIGFRKISHETNTATATLRLPATLKVTA 136
Query: 454 VVEWMT*KMEKLRQNASTPEPRMIVSAGTMESCV*NQFITPPPSS 588
VVEW+T*KMEK RQNASTPE RMI+S ESCV*NQ ITPPPSS
Sbjct: 135 VVEWIT*KMEKFRQNASTPELRMIISEEKTESCV*NQSITPPPSS 1
Score = 142 bits (305), Expect = 4e-033
Identities = 57/104 (54%), Positives = 76/104 (73%)
Frame = +3 / -1
Query: 276 GCSLALRDQMPHIAIESATNQGDSTPDCIHCRDWIPEDRPRH*HCHRDFKVACNVERHCC 455
G SL L D+MPHI ++S ++ D DCIH D +PED+PR+ H + +FKVA N+E HC
Sbjct: 313 GSSLLLGDEMPHIPVKSTADERDCASDCIHWGDRVPEDQPRNQHGNGNFKVASNIEGHCG 134
Query: 456 C*MDDVEDGEVKAERKHPRT*DDSQRRNDGIMCVEPVHYTSSFK 587
MD +EDGEV+A+ KHP *DD+QRR D ++CVEPV+Y +SFK
Sbjct: 133 GRMDHIEDGEVQAKCKHP*A*DDNQRRKDRVVCVEPVNYATSFK 2
Score = 111 bits (237), Expect = 8e-024
Identities = 59/133 (44%), Positives = 71/133 (53%)
Frame = -2 / +1
Query: 588 A*RRRCNELVLHT*FHRSCADYHPRFGGACVXXXXXXXXXXPFNNSSDVQRCRQP*SRXX 409
A*RR N LVLHT ADYHP+ GAC+ PF++ SD+Q C QP*S
Sbjct: 1 A*RRWRN*LVLHTRLCLFFADYHPKLRGACILPELLHLLCDPFDHRSDLQCCWQP*SCRC 180
Query: 408 XXXXXVDLPESNLCNECNRVWNHPGWLHFLWLCEASDLAAPGCSPRESRNSASKESDGDA 229
DLPE +L NECNR+ NH L+FL +CEAS L GC P + E G
Sbjct: 181 RVGFVADLPEPDLPNECNRMRNHARRLYFLRVCEASHLPTEGCCPSWEPRNELAEKSGGD 360
Query: 228 SPCCGREARKGLA 190
+P C + R GLA
Sbjct: 361 APPCR*QGR*GLA 399
Score = 34.1 bits (68), Expect(2) = 9e-062
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -3 / +2
Query: 293 QRQAAAPGSLGTAQARNQTEMLPLVVDEKQE 201
Q+ AA GS GT R++ EMLPLV D++ +
Sbjct: 299 QKAAAPLGSQGTNSPRSRVEMLPLVGDKEDK 391
Score = 27.2 bits (53), Expect(2) = 7e-040
Identities = 15/27 (55%), Positives = 15/27 (55%)
Frame = +1 / -2
Query: 214 STTRGSISV*FLACAVPRLPGAAAWRC 294
S TRGSIS L VP LP AA C
Sbjct: 378 SPTRGSISTRLLGEFVPWLPRGAAAFC 298
>gb|BM373085.2|BM373085 EBma04_SQ003_A16_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ003_A16 5', mRNA sequence
Length = 619
Score = 224 bits (483), Expect(2) = 9e-062
Identities = 92/105 (87%), Positives = 98/105 (93%)
Frame = -3 / +2
Query: 587 LEGGGVMNWFYTHDSIVPALTIILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVA 408
LEGGGV++WFYTHDS+ +L IIL SGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVA
Sbjct: 2 LEGGGVIDWFYTHDSVFSSLIIILSSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVA 181
Query: 407 VLVSWSIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQRQAAAP 273
VLVSW IFRNPIS MNAIGC ITLVGCTFYGYVRHLISQ++AAAP
Sbjct: 182 VLVSWLIFRNPISPMNAIGCAITLVGCTFYGYVRHLISQQKAAAP 316
Score = 157 bits (338), Expect(2) = 7e-040
Identities = 71/105 (67%), Positives = 76/105 (72%)
Frame = +1 / -2
Query: 274 GAAAWRCEIRCLT*P*KVQPTRVIPHPIAFIAEIGFRKIDHDXXXXXXXXXXXXXXNVTA 453
GAAA+ E+RCLT P*KVQPT VI HPIAFI EIGFRKI H+ VTA
Sbjct: 315 GAAAFCWEMRCLTYP*KVQPTSVIAHPIAFIGEIGFRKISHETNTATATLRLPATLKVTA 136
Query: 454 VVEWMT*KMEKLRQNASTPEPRMIVSAGTMESCV*NQFITPPPSS 588
VVEW+T*KMEK RQNASTPE RMI+S ESCV*NQ ITPPPSS
Sbjct: 135 VVEWIT*KMEKFRQNASTPELRMIISEEKTESCV*NQSITPPPSS 1
Score = 140 bits (301), Expect = 1e-032
Identities = 56/104 (53%), Positives = 75/104 (72%)
Frame = +3 / -1
Query: 276 GCSLALRDQMPHIAIESATNQGDSTPDCIHCRDWIPEDRPRH*HCHRDFKVACNVERHCC 455
G S L D+MPHI ++S ++ D DCIH D +PED+PR+ H + +FKVA N+E HC
Sbjct: 313 GSSFLLGDEMPHIPVKSTADERDCASDCIHWGDRVPEDQPRNQHGNGNFKVASNIEGHCG 134
Query: 456 C*MDDVEDGEVKAERKHPRT*DDSQRRNDGIMCVEPVHYTSSFK 587
MD +EDGEV+A+ KHP *DD+QRR D ++CVEPV+Y +SFK
Sbjct: 133 GRMDHIEDGEVQAKCKHP*A*DDNQRRKDRVVCVEPVNYATSFK 2
Score = 108 bits (231), Expect = 6e-023
Identities = 58/133 (43%), Positives = 70/133 (52%)
Frame = -2 / +1
Query: 588 A*RRRCNELVLHT*FHRSCADYHPRFGGACVXXXXXXXXXXPFNNSSDVQRCRQP*SRXX 409
A*RR N LVLHT ADYHP+ GAC+ PF++ SD+Q C QP*S
Sbjct: 1 A*RRWRN*LVLHTRLCLFFADYHPKLRGACILPELLHLLCDPFDHRSDLQCCWQP*SCRC 180
Query: 408 XXXXXVDLPESNLCNECNRVWNHPGWLHFLWLCEASDLAAPGCSPRESRNSASKESDGDA 229
DLPE +L NECNR+ NH L+FL +CEAS L C P + E G
Sbjct: 181 RVGFVADLPEPDLPNECNRMRNHARRLYFLRVCEASHLPTESCCPSWEPRNELAEKSGGD 360
Query: 228 SPCCGREARKGLA 190
+P C + R GLA
Sbjct: 361 APPCR*QGR*GLA 399
Score = 34.1 bits (68), Expect(2) = 9e-062
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -3 / +2
Query: 293 QRQAAAPGSLGTAQARNQTEMLPLVVDEKQE 201
Q+ AA GS GT R++ EMLPLV D++ +
Sbjct: 299 QKAAAPLGSQGTNSPRSRVEMLPLVGDKEDK 391
Score = 27.2 bits (53), Expect(2) = 7e-040
Identities = 15/27 (55%), Positives = 15/27 (55%)
Frame = +1 / -2
Query: 214 STTRGSISV*FLACAVPRLPGAAAWRC 294
S TRGSIS L VP LP AA C
Sbjct: 378 SPTRGSISTRLLGEFVPWLPRGAAAFC 298
>gb|BU987907.1|BU987907 HF16B16r HF Hordeum vulgare subsp. vulgare cDNA clone HF16B16
5-PRIME, mRNA sequence
Length = 641
Score = 214 bits (462), Expect(2) = 7e-059
Identities = 88/100 (88%), Positives = 94/100 (94%)
Frame = -3 / +1
Query: 572 VMNWFYTHDSIVPALTIILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVAVLVSW 393
V++WFYTHDS+ +L IILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVAVLVSW
Sbjct: 1 VIDWFYTHDSVFSSLIIILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVAVLVSW 180
Query: 392 SIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQRQAAAP 273
IFRNPIS MNAIGC ITLVGCTFYGYVRHLISQ++AAAP
Sbjct: 181 LIFRNPISPMNAIGCAITLVGCTFYGYVRHLISQQKAAAP 300
Score = 146 bits (314), Expect(2) = 1e-036
Identities = 66/99 (66%), Positives = 71/99 (71%)
Frame = +1 / -1
Query: 274 GAAAWRCEIRCLT*P*KVQPTRVIPHPIAFIAEIGFRKIDHDXXXXXXXXXXXXXXNVTA 453
GAAA+ E+RCLT P*KVQPT VI HPIAFI EIGFRKI H+ VTA
Sbjct: 299 GAAAFCWEMRCLTYP*KVQPTSVIAHPIAFIGEIGFRKISHETNTATATLRLPATLKVTA 120
Query: 454 VVEWMT*KMEKLRQNASTPEPRMIVSAGTMESCV*NQFI 570
VVEW+T*KMEK RQNASTPEPRMI+S ESCV*NQ I
Sbjct: 119 VVEWIT*KMEKFRQNASTPEPRMIISEEKTESCV*NQSI 3
Score = 135 bits (289), Expect = 6e-031
Identities = 54/99 (54%), Positives = 72/99 (72%)
Frame = +3 / -3
Query: 276 GCSLALRDQMPHIAIESATNQGDSTPDCIHCRDWIPEDRPRH*HCHRDFKVACNVERHCC 455
G SL L D+MPHI ++S ++ D DCIH D +PED+PR+ H + +FKVA N+E HC
Sbjct: 297 GSSLLLGDEMPHIPVKSTADERDCASDCIHWGDRVPEDQPRNQHGNGNFKVASNIEGHCG 118
Query: 456 C*MDDVEDGEVKAERKHPRT*DDSQRRNDGIMCVEPVHY 572
MD +EDGEV+A+ KHP *DD+QRR D ++CVEPV+Y
Sbjct: 117 GRMDHIEDGEVQAKCKHP*A*DDNQRRKDRVVCVEPVNY 1
Score = 108 bits (231), Expect = 6e-023
Identities = 56/127 (44%), Positives = 67/127 (52%)
Frame = -2 / +3
Query: 570 NELVLHT*FHRSCADYHPRFGGACVXXXXXXXXXXPFNNSSDVQRCRQP*SRXXXXXXXV 391
N LVLHT ADYHPR GAC+ PF++ SD+Q C QP*S
Sbjct: 3 N*LVLHTRLCLFFADYHPRLRGACILPELLHLLCDPFDHRSDLQCCWQP*SCRCRVGFVA 182
Query: 390 DLPESNLCNECNRVWNHPGWLHFLWLCEASDLAAPGCSPRESRNSASKESDGDASPCCGR 211
DLPE +L NECNR+ NH L+FL +CEAS L GC P + E G +P C
Sbjct: 183 DLPEPDLPNECNRMRNHARRLYFLRVCEASHLPTEGCCPSWEPRNELAEKSGGDAPPCR* 362
Query: 210 EARKGLA 190
+ R GLA
Sbjct: 363 QGR*GLA 383
Score = 34.1 bits (68), Expect(2) = 7e-059
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -3 / +1
Query: 293 QRQAAAPGSLGTAQARNQTEMLPLVVDEKQE 201
Q+ AA GS GT R++ EMLPLV D++ +
Sbjct: 283 QKAAAPLGSQGTNSPRSRVEMLPLVGDKEDK 375
Score = 27.2 bits (53), Expect(2) = 1e-036
Identities = 15/27 (55%), Positives = 15/27 (55%)
Frame = +1 / -1
Query: 214 STTRGSISV*FLACAVPRLPGAAAWRC 294
S TRGSIS L VP LP AA C
Sbjct: 362 SPTRGSISTRLLGEFVPWLPRGAAAFC 282
>gb|BU987948.1|BU987948 HF16D20r HF Hordeum vulgare subsp. vulgare cDNA clone HF16D20
5-PRIME, mRNA sequence
Length = 624
Score = 214 bits (462), Expect(2) = 7e-059
Identities = 88/100 (88%), Positives = 94/100 (94%)
Frame = -3 / +1
Query: 572 VMNWFYTHDSIVPALTIILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVAVLVSW 393
V++WFYTHDS+ +L IILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVAVLVSW
Sbjct: 1 VIDWFYTHDSVFSSLIIILGSGVLAFCLNFSIFYVIHSTTAVTFNVAGNLKVAVAVLVSW 180
Query: 392 SIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQRQAAAP 273
IFRNPIS MNAIGC ITLVGCTFYGYVRHLISQ++AAAP
Sbjct: 181 LIFRNPISPMNAIGCAITLVGCTFYGYVRHLISQQKAAAP 300
Score = 146 bits (314), Expect(2) = 1e-036
Identities = 66/99 (66%), Positives = 71/99 (71%)
Frame = +1 / -2
Query: 274 GAAAWRCEIRCLT*P*KVQPTRVIPHPIAFIAEIGFRKIDHDXXXXXXXXXXXXXXNVTA 453
GAAA+ E+RCLT P*KVQPT VI HPIAFI EIGFRKI H+ VTA
Sbjct: 299 GAAAFCWEMRCLTYP*KVQPTSVIAHPIAFIGEIGFRKISHETNTATATLRLPATLKVTA 120
Query: 454 VVEWMT*KMEKLRQNASTPEPRMIVSAGTMESCV*NQFI 570
VVEW+T*KMEK RQNASTPEPRMI+S ESCV*NQ I
Sbjct: 119 VVEWIT*KMEKFRQNASTPEPRMIISEEKTESCV*NQSI 3
Score = 135 bits (289), Expect = 6e-031
Identities = 54/99 (54%), Positives = 72/99 (72%)
Frame = +3 / -1
Query: 276 GCSLALRDQMPHIAIESATNQGDSTPDCIHCRDWIPEDRPRH*HCHRDFKVACNVERHCC 455
G SL L D+MPHI ++S ++ D DCIH D +PED+PR+ H + +FKVA N+E HC
Sbjct: 297 GSSLLLGDEMPHIPVKSTADERDCASDCIHWGDRVPEDQPRNQHGNGNFKVASNIEGHCG 118
Query: 456 C*MDDVEDGEVKAERKHPRT*DDSQRRNDGIMCVEPVHY 572
MD +EDGEV+A+ KHP *DD+QRR D ++CVEPV+Y
Sbjct: 117 GRMDHIEDGEVQAKCKHP*A*DDNQRRKDRVVCVEPVNY 1
Score = 108 bits (231), Expect = 6e-023
Identities = 56/127 (44%), Positives = 67/127 (52%)
Frame = -2 / +3
Query: 570 NELVLHT*FHRSCADYHPRFGGACVXXXXXXXXXXPFNNSSDVQRCRQP*SRXXXXXXXV 391
N LVLHT ADYHPR GAC+ PF++ SD+Q C QP*S
Sbjct: 3 N*LVLHTRLCLFFADYHPRLRGACILPELLHLLCDPFDHRSDLQCCWQP*SCRCRVGFVA 182
Query: 390 DLPESNLCNECNRVWNHPGWLHFLWLCEASDLAAPGCSPRESRNSASKESDGDASPCCGR 211
DLPE +L NECNR+ NH L+FL +CEAS L GC P + E G +P C
Sbjct: 183 DLPEPDLPNECNRMRNHARRLYFLRVCEASHLPTEGCCPSWEPRNELAEKSGGDAPPCR* 362
Query: 210 EARKGLA 190
+ R GLA
Sbjct: 363 QGR*GLA 383
Score = 34.1 bits (68), Expect(2) = 7e-059
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -3 / +1
Query: 293 QRQAAAPGSLGTAQARNQTEMLPLVVDEKQE 201
Q+ AA GS GT R++ EMLPLV D++ +
Sbjct: 283 QKAAAPLGSQGTNSPRSRVEMLPLVGDKEDK 375
Score = 27.2 bits (53), Expect(2) = 1e-036
Identities = 15/27 (55%), Positives = 15/27 (55%)
Frame = +1 / -2
Query: 214 STTRGSISV*FLACAVPRLPGAAAWRC 294
S TRGSIS L VP LP AA C
Sbjct: 362 SPTRGSISTRLLGEFVPWLPRGAAAFC 282
>gb|CA005867.1|CA005867 HU08H01u HU Hordeum vulgare subsp. vulgare cDNA clone HU08H01
3-PRIME, mRNA sequence
Length = 484
Score = 139 bits (298), Expect(2) = 2e-036
Identities = 59/66 (89%), Positives = 62/66 (93%)
Frame = -3 / -1
Query: 470 VIHSTTAVTFNVAGNLKVAVAVLVSWSIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQ 291
VIHSTTAVTFNVAGNLKVAVAVLVSW IFRNPIS MNAIGC ITLVGCTF GY+RHLISQ
Sbjct: 484 VIHSTTAVTFNVAGNLKVAVAVLVSWLIFRNPISPMNAIGCTITLVGCTFCGYLRHLISQ 305
Query: 290 RQAAAP 273
++AAAP
Sbjct: 304 QKAAAP 287
Score = 34.1 bits (68), Expect(2) = 2e-036
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -3 / -1
Query: 293 QRQAAAPGSLGTAQARNQTEMLPLVVDEKQE 201
Q+ AA GS GT R++ EMLPLV D++ +
Sbjct: 304 QKAAAPLGSQGTNSPRSRVEMLPLVGDKEDK 212
>gb|BE216718.2|BE216718 HV_CEb0011F09f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0011F09f, mRNA sequence
Length = 867
Score = 137 bits (293), Expect = 2e-031
Identities = 61/182 (33%), Positives = 101/182 (55%)
Frame = -3 / +2
Query: 1217 GGKAPAGLGTLRAVLAILQWWGFNVTVIIMNKWIFQKLDFKFPLTVSCVHFICSSIGAYI 1038
GG A G V + W+ N+ V+++NK++ FK+P+ ++ H ++ +Y
Sbjct: 122 GGAGAAANGRFFTVGLVTAWYSSNIGVLLLNKYLLSNYGFKYPIFLTMCHMSACALFSYA 301
Query: 1037 AIHVLKAKPLIQVEPEDRWKRIFPMSFVFCINIVLGNVSLRYIPVSFMQTIKSFTPATTV 858
AI L+ P+ V + +I +S VFC ++V GNVSLRY+PVSF Q + + TP T
Sbjct: 302 AIAWLRIVPMQLVRSRVQLAKISALSLVFCGSVVSGNVSLRYLPVSFNQAVGATTPFFTA 481
Query: 857 ILQWLVWSKHFEWRIWASLVPIVGGILLTSVTELSFNIFGFCAAMVGCLATSTKTILAES 678
+ +++ K W + +LVP+V G+++ S E SF++FGF + A + KT+L
Sbjct: 482 VFAYIMTVKRESWITYLTLVPVVTGVVIASGGEPSFHLFGFIMCIGTTAARALKTVLQGI 661
Query: 677 LL 672
LL
Sbjct: 662 LL 667
>gb|CA007353.1|CA007353 HU07K19r HU Hordeum vulgare subsp. vulgare cDNA clone HU07K19
5-PRIME, mRNA sequence
Length = 176
Score = 128 bits (274), Expect = 7e-029
Identities = 49/54 (90%), Positives = 51/54 (94%)
Frame = -3 / +1
Query: 1127 NKWIFQKLDFKFPLTVSCVHFICSSIGAYIAIHVLKAKPLIQVEPEDRWKRIFP 966
N WIFQKLDFKFPLTVSC+HFICSSI AY+AIHV KAKPLIQVEPEDRWKRIFP
Sbjct: 13 NTWIFQKLDFKFPLTVSCIHFICSSIRAYVAIHVFKAKPLIQVEPEDRWKRIFP 174
Score = 107 bits (228), Expect = 1e-022
Identities = 45/57 (78%), Positives = 49/57 (85%)
Frame = +1 / -1
Query: 964 IGNILFQRSSGST*IRGFALRTWIAM*APMEEQMKWTQDTVRGNLKSSFWKIHLFMM 1134
+G ILFQRSSGST*I GFAL TWIA *A MEE MKW QDTVRGNLKSSFWKI +F++
Sbjct: 176 MGKILFQRSSGST*ISGFALNTWIAT*ALMEEHMKWMQDTVRGNLKSSFWKIQVFLV 6
>gb|BG368245.2|BG368245 HVSMEi0017B05f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0017B05f, mRNA sequence
Length = 779
Score = 124 bits (266), Expect = 8e-028
Identities = 53/78 (67%), Positives = 59/78 (75%)
Frame = -3 / +2
Query: 1199 GLGTLRAVLAILQWWGFNVTVIIMNKWIFQKLDFKFPLTVSCVHFICSSIGAYIAIHVLK 1020
GLG +RAVLAILQ WG TVI +N WIFQKLD K PLT SCVHFIC SI Y IH+LK
Sbjct: 227 GLGRMRAVLAILQRWGLKRTVININNWIFQKLDSKVPLTGSCVHFICPSIRGYATIHLLK 406
Query: 1019 AKPLIQVEPEDRWKRIFP 966
AKPLI+ +P DR K+ FP
Sbjct: 407 AKPLIKAKP*DRRKKNFP 460
Score = 111 bits (238), Expect = 6e-024
Identities = 47/79 (59%), Positives = 54/79 (68%)
Frame = +1 / -3
Query: 964 IGNILFQRSSGST*IRGFALRTWIAM*APMEEQMKWTQDTVRGNLKSSFWKIHLFMMMTV 1143
+G F RS G I+GFAL WI * +E MKWTQD V+G L+SSFWKI L M+MTV
Sbjct: 462 LGKFFFLRS*GLALIKGFALSRWIVA*PLIEGHMKWTQDPVKGTLESSFWKIQLLMLMTV 283
Query: 1144 TLKPHHWRMASTARRVPRP 1200
KPH WRMASTAR +P P
Sbjct: 282 RFKPHLWRMASTARILPSP 226
>gb|AJ462714.1|AJ462714 AJ462714 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200017A12F1, mRNA sequence
Length = 360
Score = 106 bits (227), Expect(2) = 9e-027
Identities = 43/49 (87%), Positives = 46/49 (93%)
Frame = -3 / +3
Query: 419 VAVAVLVSWSIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQRQAAAP 273
VAVAVLVSW IFRNPIS MNAIGC ITL+GCTFYGYVRHLISQ++AAAP
Sbjct: 9 VAVAVLVSWLIFRNPISPMNAIGCAITLIGCTFYGYVRHLISQQKAAAP 155
Score = 34.1 bits (68), Expect(2) = 9e-027
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -3 / +3
Query: 293 QRQAAAPGSLGTAQARNQTEMLPLVVDEKQE 201
Q+ AA GS GT R++ EMLPLV D++ +
Sbjct: 138 QKAAAPLGSQGTNSPRSRVEMLPLVGDKEDK 230
>gb|BU999143.1|BU999143 HI13G13r HI Hordeum vulgare subsp. vulgare cDNA clone HI13G13
5-PRIME, mRNA sequence
Length = 522
Score = 114 bits (244), Expect = 9e-025
Identities = 48/147 (32%), Positives = 82/147 (55%)
Frame = -3 / +1
Query: 1217 GGKAPAGLGTLRAVLAILQWWGFNVTVIIMNKWIFQKLDFKFPLTVSCVHFICSSIGAYI 1038
GG A G V + W+ N+ V+++NK++ FK+P+ ++ H ++ +Y
Sbjct: 82 GGAGAAANGRFFTVGLVTAWYSSNIGVLLLNKYLLSNYGFKYPIFLTMCHMSACALFSYA 261
Query: 1037 AIHVLKAKPLIQVEPEDRWKRIFPMSFVFCINIVLGNVSLRYIPVSFMQTIKSFTPATTV 858
AI L+ P+ V + +I +S VFC ++V GNVSLRY+PVSF Q + + TP T
Sbjct: 262 AIAWLRIVPMQLVRSRVQLAKISALSLVFCGSVVSGNVSLRYLPVSFNQAVGATTPFFTA 441
Query: 857 ILQWLVWSKHFEWRIWASLVPIVGGIL 777
+ +++ K W + +LVP+V G++
Sbjct: 442 VFAYIMTVKRESWITYLTLVPVVTGVV 522
>gb|BE215201.1|BE215201 HV_CEb0006C07f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0006C07f, mRNA sequence
Length = 679
Score = 113 bits (241), Expect = 2e-024
Identities = 51/143 (35%), Positives = 83/143 (58%)
Frame = -3 / +2
Query: 1100 FKFPLTVSCVHFICSSIGAYIAIHVLKAKPLIQVEPEDRWKRIFPMSFVFCINIVLGNVS 921
FK+P+ ++ H ++ +Y AI L+ P+ V + +I +S VFC ++V GNVS
Sbjct: 38 FKYPIFLTMCHMSACALFSYAAIAWLRIVPMQLVRSRVQLAKISALSLVFCGSVVSGNVS 217
Query: 920 LRYIPVSFMQTIKSFTPATTVILQWLVWSKHFEWRIWASLVPIVGGILLTSVTELSFNIF 741
LRY+PVSF Q + + TP T + +++ K W + +LVP+V G+++ S E SF++F
Sbjct: 218 LRYLPVSFNQAVGATTPFFTAVFAYIMTVKRESWITYLTLVPVVTGVVIASGGEPSFHLF 397
Query: 740 GFCAAMVGCLATSTKTILAESLL 672
GF + A + KT+L LL
Sbjct: 398 GFIMCIGATAARALKTVLQGILL 466
>gb|BF255677.2|BF255677 HVSMEf0007I02f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEf0007I02f, mRNA sequence
Length = 838
Score = 106 bits (227), Expect = 2e-022
Identities = 49/140 (35%), Positives = 80/140 (57%)
Frame = -3 / +2
Query: 1091 PLTVSCVHFICSSIGAYIAIHVLKAKPLIQVEPEDRWKRIFPMSFVFCINIVLGNVSLRY 912
P+ ++ H ++ +Y AI L+ P+ V + +I +S VFC ++V GNVSLRY
Sbjct: 2 PIFLTMCHMSACALFSYAAIAWLRIVPMQLVRSRVQLAKISALSLVFCGSVVSGNVSLRY 181
Query: 911 IPVSFMQTIKSFTPATTVILQWLVWSKHFEWRIWASLVPIVGGILLTSVTELSFNIFGFC 732
+PVSF Q + + TP T + +++ K W + +LVP+V G+++ S E SF++FGF
Sbjct: 182 LPVSFNQAVGATTPFFTAVFAYIMTVKRESWITYLTLVPVVTGVVIASGGEPSFHLFGFI 361
Query: 731 AAMVGCLATSTKTILAESLL 672
+ A + KT+L LL
Sbjct: 362 MCIGATAARALKTVLQGILL 421
>gb|CA027915.1|CA027915 HZ60G23r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ60G23
5-PRIME, mRNA sequence
Length = 472
Score = 104 bits (221), Expect = 1e-021
Identities = 40/46 (86%), Positives = 45/46 (97%)
Frame = -3 / -2
Query: 425 LKVAVAVLVSWSIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQR 288
++VAVAVL+SW IFRNPISAMNA+GCGITLVGCTFYGYVRHLISQ+
Sbjct: 399 MQVAVAVLISWMIFRNPISAMNAVGCGITLVGCTFYGYVRHLISQQ 262
>gb|CA028055.1|CA028055 HZ60N16r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ60N16
5-PRIME, mRNA sequence
Length = 454
Score = 104 bits (221), Expect = 1e-021
Identities = 40/46 (86%), Positives = 45/46 (97%)
Frame = -3 / -3
Query: 425 LKVAVAVLVSWSIFRNPISAMNAIGCGITLVGCTFYGYVRHLISQR 288
++VAVAVL+SW IFRNPISAMNA+GCGITLVGCTFYGYVRHLISQ+
Sbjct: 413 MQVAVAVLISWMIFRNPISAMNAVGCGITLVGCTFYGYVRHLISQQ 276
>gb|CK124312.1|CK124312 BES1824108f09 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010F098 5-PRIME, mRNA sequence
Length = 309
Score = 88.6 bits (187), Expect(2) = 2e-021
Identities = 35/39 (89%), Positives = 37/39 (94%)
Frame = -3 / +2
Query: 389 IFRNPISAMNAIGCGITLVGCTFYGYVRHLISQRQAAAP 273
IFRNPIS MNAIGC ITLVGCTFYGYVRHLISQ++AAAP
Sbjct: 5 IFRNPISPMNAIGCAITLVGCTFYGYVRHLISQQKAAAP 121
Score = 34.1 bits (68), Expect(2) = 2e-021
Identities = 15/31 (48%), Positives = 21/31 (67%)
Frame = -3 / +2
Query: 293 QRQAAAPGSLGTAQARNQTEMLPLVVDEKQE 201
Q+ AA GS GT R++ EMLPLV D++ +
Sbjct: 104 QKAAAPLGSQGTNSPRSRVEMLPLVGDKEDK 196
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 659,937,345
Number of Sequences: 312970
Number of extensions: 11886830
Number of successful extensions: 76903
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 15546166068
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)