TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2485885.2.1
(1063 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA023704.1|CA023704 HZ47C08r HZ Hordeum vulgare subsp. v... 216 1e-055
gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedl... 132 3e-046
gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. v... 95 3e-043
gb|BI956785.1|BI956785 HVSMEn0005E22f Hordeum vulgare rachi... 93 4e-037
gb|AL450622.1|AL450622 AL450622 Hordeum vulgare Barke etiol... 117 2e-036
gb|CV063917.1|CV063917 BNEL94f3 Barley EST endosperm librar... 99 1e-034
gb|CV059888.1|CV059888 BNEL52a6 Barley EST endosperm librar... 92 1e-032
gb|CV058275.1|CV058275 BNEL36c3 Barley EST endosperm librar... 92 1e-031
gb|BF623637.2|BF623637 HVSMEa0003E04f Hordeum vulgare seedl... 91 3e-031
gb|BM443110.2|BM443110 EBro02_SQ002_J16_R root, 3 week, hyd... 89 2e-030
gb|CA009114.1|CA009114 HU13D11r HU Hordeum vulgare subsp. v... 94 7e-030
emb|AJ496572.1|HVU496572 Hordeum vulgare partial mRNA for p... 88 1e-029
gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedl... 88 2e-028
gb|CA015634.1|CA015634 HT14O18r HT Hordeum vulgare subsp. v... 86 4e-028
gb|BF622731.2|BF622731 HVSMEa0008E02f Hordeum vulgare seedl... 75 2e-027
gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hyd... 122 3e-027
gb|BF624531.2|BF624531 HVSMEa0017A18f Hordeum vulgare seedl... 82 5e-026
gb|CA028126.1|CA028126 HZ61B05r HZ Hordeum vulgare subsp. v... 80 2e-025
gb|BM101456.2|BM101456 EBpi01_SQ003_O13_R pistil, 1 DPA, no... 67 7e-024
gb|AV927070.1|AV927070 AV927070 K. Sato unpublished cDNA li... 111 1e-023
gb|AV931900.1|AV931900 AV931900 K. Sato unpublished cDNA li... 111 1e-023
gb|AV929760.1|AV929760 AV929760 K. Sato unpublished cDNA li... 73 1e-023
gb|AV927784.1|AV927784 AV927784 K. Sato unpublished cDNA li... 73 1e-023
gb|BM816260.1|BM816260 HC113E04_SK.ab1 HC Hordeum vulgare s... 72 2e-022
gb|BM816841.1|BM816841 HB105A11_SK.ab1 HB Hordeum vulgare s... 74 4e-022
gb|BF256192.2|BF256192 HVSMEf0009B02f Hordeum vulgare seedl... 105 4e-022
gb|BI953418.1|BI953418 HVSMEm0012J18f Hordeum vulgare green... 82 1e-021
gb|BF622661.2|BF622661 HVSMEa0007K11f Hordeum vulgare seedl... 68 3e-021
gb|BQ466592.1|BQ466592 HT02P16r HT Hordeum vulgare subsp. v... 66 3e-021
gb|AJ462599.1|AJ462599 AJ462599 S00002 Hordeum vulgare subs... 102 3e-021
gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedl... 62 4e-021
gb|BF628779.2|BF628779 HVSMEb0008B22f Hordeum vulgare seedl... 63 4e-021
gb|AV833284.1|AV833284 AV833284 K. Sato unpublished cDNA li... 70 4e-021
gb|BJ463253.1|BJ463253 BJ463253 K. Sato unpublished cDNA li... 70 4e-021
gb|BJ450621.1|BJ450621 BJ450621 K. Sato unpublished cDNA li... 101 7e-021
gb|BJ458043.1|BJ458043 BJ458043 K. Sato unpublished cDNA li... 101 7e-021
gb|CA001229.1|CA001229 HS18N07u HS Hordeum vulgare subsp. v... 101 7e-021
>gb|CA023704.1|CA023704 HZ47C08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ47C08
5-PRIME, mRNA sequence
Length = 355
Score = 216 bits (467), Expect = 1e-055
Identities = 89/111 (80%), Positives = 97/111 (87%)
Frame = +1 / +2
Query: 280 PSDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGV 459
PSD +L ELLP FL RTK++G+VWPTKAPQKEILAHA+VGGFVTH GWNS LESLW GV
Sbjct: 20 PSDADLGELLPEGFLERTKEKGMVWPTKAPQKEILAHASVGGFVTHGGWNSTLESLWFGV 199
Query: 460 PMVPWPLGAEQHYNAFTLVADMGVAVALNVERKRKNFVEATELERAVKALM 612
PMVPWPL AEQH NAFTLVA MGVAVA+ V+RKR NFV A+ELERAVKALM
Sbjct: 200 PMVPWPLYAEQHLNAFTLVAYMGVAVAMEVDRKRNNFVAASELERAVKALM 352
Score = 154 bits (330), Expect = 1e-036
Identities = 66/111 (59%), Positives = 78/111 (70%)
Frame = -2 / -1
Query: 612 HQSFNRSLQLGRLHEILPLPFHVQRHGDAHVGHQRERVVVLLGAKRPWHHRHAMPEALED 433
H+ + LQL R E++PLP H+ HG AHVGHQRERV VLL +RPWHHRHA P+AL+
Sbjct: 352 HERLHGPLQLRRGDEVVPLPVHLHGHGHAHVGHQRERVQVLLRVQRPWHHRHAEPQALQG 173
Query: 432 RVPATVRDEASHGGVGEYLLLRRLGRPHEPSILRPRQEIRRKQLREVPVRG 280
RVP VR EA H GV + LLLR LGRPH +L P Q+ R+QL EV VRG
Sbjct: 172 RVPPAVRHEAPHRGVRQDLLLRSLGRPHHALLLCPLQKPLREQLPEVRVRG 20
Score = 152 bits (327), Expect = 3e-036
Identities = 70/109 (64%), Positives = 72/109 (66%)
Frame = -3 / -2
Query: 605 ALTARSSSVXXXXXXXXXXXXXXXXTPMSATSVNAL*CCSAPSGHGTIGTPCQRLSRTEF 426
A TARSSS TPM ATSVNA CCSA SGHGTIGTP RLSR EF
Sbjct: 345 AFTARSSSDAATKLFLFLSTSMATATPM*ATSVNAFRCCSAYSGHGTIGTPNHRLSRVEF 166
Query: 425 QPQCVTKPPTAAWASISFCGALVGHTSPLSFVLAKKSAGSSSARFPSEG 279
P CVTKPPT A A ISFCGALVGHT P SFVL+K +GSSS R SEG
Sbjct: 165 HPPCVTKPPTEACARISFCGALVGHTMPFSFVLSKNPSGSSSPRSASEG 19
>gb|BG300446.1|BG300446 HVSMEb0017B13f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017B13f, mRNA sequence
Length = 875
Score = 132 bits (283), Expect(2) = 3e-046
Identities = 56/101 (55%), Positives = 72/101 (71%)
Frame = +1 / +3
Query: 280 PSDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGV 459
P + +L LLP FLART++RGLV T APQ+++LAH AVGGFVTHCGWNSVLE++ GV
Sbjct: 171 PPEPDLDALLPPGFLARTQERGLVVKTWAPQRDVLAHGAVGGFVTHCGWNSVLEAVMAGV 350
Query: 460 PMVPWPLGAEQHYNAFTLVADMGVAVALNVERKRKNFVEAT 582
PM+ WPL AEQ N L ++G+A A++ K V+AT
Sbjct: 351 PMLAWPLYAEQRLNRVFLEKELGLAAAVDRYYHYKELVDAT 473
Score = 92.7 bits (196), Expect(2) = 1e-029
Identities = 45/84 (53%), Positives = 52/84 (61%)
Frame = -3 / -2
Query: 527 PMSATSVNAL*CCSAPSGHGTIGTPCQRLSRTEFQPQCVTKPPTAAWASISFCGALVGHT 348
P S + L CSA SGH +IGTP S TEF PQCVT PPTA AS S CGA V T
Sbjct: 418 PSSFSRNTRLSRCSAYSGHASIGTPAITASSTEFHPQCVTNPPTAPCASTSRCGAHVFTT 239
Query: 347 SPLSFVLAKKSAGSSSARFPSEGN 276
SPLS+V A+K G ++R S G+
Sbjct: 238 SPLSWVRARKPGGRRASRSGSGGS 167
Score = 73.5 bits (154), Expect(2) = 3e-046
Identities = 30/46 (65%), Positives = 35/46 (76%)
Frame = +1 / +3
Query: 124 DSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLRGTP 261
D+QP SVVFLCFGS G F+A Q EIA GL+ SG+RFLWV+R P
Sbjct: 3 DAQPEGSVVFLCFGSLGVFSAAQIREIAVGLEASGNRFLWVVRNPP 140
Score = 57.4 bits (119), Expect(2) = 1e-029
Identities = 25/43 (58%), Positives = 27/43 (62%)
Frame = -3 / -2
Query: 251 RSTHRNRCPLRSRPCAISCAWGAVKQPPLPKHRNTTELGGCES 123
R+THRNR PL S P AIS A+ P LPK RNTT GC S
Sbjct: 130 RTTHRNRLPLASSPTAISLICAALNTPRLPKQRNTTLPSGCAS 2
>gb|BU998386.1|BU998386 HI10O06r HI Hordeum vulgare subsp. vulgare cDNA clone HI10O06
5-PRIME, mRNA sequence
Length = 635
Score = 94.5 bits (200), Expect(3) = 3e-043
Identities = 37/66 (56%), Positives = 47/66 (71%)
Frame = +1 / +3
Query: 292 NLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVP 471
+L L+P FL RTKDRGLV + APQ ++L H A G F+THCGWNS LE + G+P++
Sbjct: 438 DLDALMPEGFLERTKDRGLVVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLPLLC 617
Query: 472 WPLGAE 489
WPL AE
Sbjct: 618 WPLYAE 635
Score = 85.4 bits (180), Expect(3) = 3e-043
Identities = 34/57 (59%), Positives = 41/57 (71%)
Frame = +1 / +3
Query: 106 ECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLRGTPEPGTK 276
+C+RWLD+QP SVVFL FGS G F++ Q EIA GLD+SG RFLWV+R P K
Sbjct: 243 DCLRWLDAQPDQSVVFLSFGSLGTFSSKQLEEIALGLDKSGERFLWVVRSPRSPDQK 413
Score = 66.1 bits (138), Expect(3) = 4e-024
Identities = 31/65 (47%), Positives = 36/65 (55%)
Frame = -3 / -2
Query: 488 SAPSGHGTIGTPCQRLSRTEFQPQCVTKPPTAAWASISFCGALVGHTSPLSFVLAKKSAG 309
SA G + G P S EFQPQCV P A W S CGA TSPLS VL+K +G
Sbjct: 634 SAYRGQHSSGNPAVMPSSVEFQPQCVMNAPVALWRRTSTCGAQDLTTSPLSLVLSKNPSG 455
Query: 308 SSSAR 294
S++R
Sbjct: 454 MSASR 440
Score = 57.0 bits (118), Expect(3) = 4e-024
Identities = 26/51 (50%), Positives = 30/51 (58%)
Frame = -3 / -2
Query: 251 RSTHRNRCPLRSRPCAISCAWGAVKQPPLPKHRNTTELGGCESSQRTHSCA 99
R+THRN P S+P AIS + P LPK RNTT GC SS R SC+
Sbjct: 388 RTTHRNLSPDLSKPSAISSSCLLENVPRLPKERNTTLWSGCASSHRRQSCS 236
Score = 34.5 bits (69), Expect(3) = 3e-043
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +1 / +3
Query: 4 AIADGRCTRGVPAPPVYSIGPVI 72
A+ DG C G PPVY IGP++
Sbjct: 150 ALNDGLCVTGRATPPVYCIGPLV 218
Score = 26.3 bits (51), Expect(3) = 4e-024
Identities = 12/37 (32%), Positives = 15/37 (40%)
Frame = -3 / -2
Query: 113 THSCACCSAGGVEGITGPIEYTGGAGTPRVHRPSAMA 3
+H CS GP++ TGG P PS A
Sbjct: 259 SHRRQSCSLSPPPETNGPMQ*TGGVARPVTQSPSLRA 149
>gb|BI956785.1|BI956785 HVSMEn0005E22f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0005E22f, mRNA sequence
Length = 846
Score = 92.7 bits (196), Expect(2) = 4e-037
Identities = 39/85 (45%), Positives = 49/85 (57%)
Frame = +1 / +2
Query: 16 GRCTRGVPAPPVYSIGPVIPSTPPAEQQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQA 195
G RG PPVY+IGP+I + C+ WLD QP SVVF+ FGSGG A
Sbjct: 14 GAGRRGPGRPPVYNIGPIIRTDAAGHAPRAACLDWLDRQPAKSVVFVSFGSGGSRAAEXM 193
Query: 196 HEIAHGLDRSGHRFLWVLRGTPEPG 270
E+A GL+ +G RFLWV+R + G
Sbjct: 194 QELALGLELNGQRFLWVVRSPSDEG 268
Score = 82.6 bits (174), Expect(2) = 4e-037
Identities = 35/58 (60%), Positives = 42/58 (72%)
Frame = +1 / +2
Query: 307 LPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPL 480
LPA F+ R+KD GL+ P+ APQ E+LAH A G V HC WNSVL+SL HGV +V PL
Sbjct: 323 LPAGFVERSKDAGLLVPSWAPQTEVLAHDATGCLVGHCRWNSVLDSLGHGVRIVA*PL 496
Score = 78.5 bits (165), Expect(2) = 5e-029
Identities = 35/88 (39%), Positives = 42/88 (47%)
Frame = -3 / -1
Query: 269 PGSGVPRSTHRNRCPLRSRPCAISCAWGAVKQPPLPKHRNTTELGGCESSQRTHSCACCS 90
P S R+THRNRCP S P A SC A + PP P TT+ GC SSQ +
Sbjct: 267 PSSLGLRTTHRNRCPFSSSPSASSCXCSAARDPPEPNETKTTDFAGCRSSQSRQAARGAW 88
Query: 89 AGGVEGITGPIEYTGGAGTPRVHRPSAM 6
+ GP+ YTGG PR P +
Sbjct: 87 PAASVRMIGPMLYTGGRPGPRRPAPGGV 4
Score = 69.8 bits (146), Expect(2) = 5e-029
Identities = 32/60 (53%), Positives = 37/60 (61%)
Frame = -3 / -1
Query: 488 SAPSGHGTIGTPCQRLSRTEFQPQCVTKPPTAAWASISFCGALVGHTSPLSFVLAKKSAG 309
S SG T+ TPC RLS TEF QC TK P A+ AS S CGA G +SP S +L+ AG
Sbjct: 504 SFKSGQATMRTPCPRLSSTEFHLQCPTKHPVASCASTSVCGAHDGTSSPASLLLSTNPAG 325
>gb|AL450622.1|AL450622 AL450622 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03H20u 5', mRNA sequence
Length = 475
Score = 117 bits (250), Expect(2) = 2e-036
Identities = 48/61 (78%), Positives = 52/61 (85%)
Frame = +1 / +2
Query: 430 SVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVALNVERKRKNFVEATELERAVKAL 609
SVLESLWHG+PM PWPL AEQH NAF LVADMGVAV L V+R+R NFVEA ELERAV+ L
Sbjct: 2 SVLESLWHGIPMAPWPLYAEQHLNAFELVADMGVAVPLKVDRRRGNFVEAAELERAVRCL 181
Query: 610 M 612
M
Sbjct: 182 M 184
Score = 89.5 bits (189), Expect(2) = 3e-026
Identities = 36/63 (57%), Positives = 48/63 (76%)
Frame = -2 / -1
Query: 618 VAHQSFNRSLQLGRLHEILPLPFHVQRHGDAHVGHQRERVVVLLGAKRPWHHRHAMPEAL 439
+AHQ+ +R LQL RLHE+ P P H++RHGDAHVG + ERV VLL + P HR+A+P+AL
Sbjct: 190 LAHQAPHRPLQLRRLHEVAPPPVHLERHGDAHVGDELERVEVLLRVEWPRRHRYAVPQAL 11
Query: 438 EDR 430
+ R
Sbjct: 10 QHR 2
Score = 55.6 bits (115), Expect(2) = 2e-036
Identities = 23/46 (50%), Positives = 32/46 (69%)
Frame = +1 / +2
Query: 619 GETARKVRDKVMEIKAACRKAMEEGGSSNMSLQRLCDALVEGAVHP 756
GE R R+K ++ CRKA+++GGSS+ +LQRL +AL GAV P
Sbjct: 185 GEEGRTAREKAAGMRDVCRKAVDKGGSSDAALQRLSEALHHGAVAP 322
Score = 49.6 bits (102), Expect(2) = 3e-026
Identities = 23/49 (46%), Positives = 27/49 (55%)
Frame = -3 / -2
Query: 764 HFPGCTAPSTSASQSLCNDMFDDPPSSIAFRHAAFISMTLSLTFLAVSP 618
H G TAP SAS+SLCN ++PP S A R + I SL L SP
Sbjct: 330 HMVGATAPWWSASESLCNAASEEPPLSTALRQTSRIPAAFSLAVLPSSP 184
>gb|CV063917.1|CV063917 BNEL94f3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL94f3 5' similar to Zea mays
cis-zeatin O-glucosyltransferase 2, mRNA sequence
Length = 640
Score = 99.1 bits (210), Expect(2) = 1e-034
Identities = 40/98 (40%), Positives = 59/98 (60%)
Frame = +1 / +1
Query: 307 LPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGA 486
L +DF RT+ RGLV APQ EILAH A FV+HCGWNS+LE L HG P++ WP+ +
Sbjct: 346 LMSDFTKRTEGRGLVITGWAPQLEILAHGATAFFVSHCGWNSLLEGLSHGKPILAWPMHS 525
Query: 487 EQHYNAFTLVADMGVAVALNVERKRKNFVEATELERAV 600
+Q +NA + + + + K + + A E++ +
Sbjct: 526 DQPWNAXYVCGHLKAGIVMRPWEKSRETLPAKEIQEVI 639
Score = 68.0 bits (142), Expect(2) = 1e-034
Identities = 26/53 (49%), Positives = 32/53 (60%)
Frame = +1 / +1
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
Q EC+ WLD QPPSSV++LCFG+ Q E+A L RF+WVLR
Sbjct: 133 QPRHECMDWLDKQPPSSVLYLCFGTMSSLPGKQIEELAGALQSCEQRFIWVLR 291
>gb|CV059888.1|CV059888 BNEL52a6 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL52a6 5' similar to Zea mays
cis-zeatin O-glucosyltransferase 2, mRNA sequence
Length = 603
Score = 92.3 bits (195), Expect(2) = 1e-032
Identities = 37/66 (56%), Positives = 47/66 (71%)
Frame = +1 / +1
Query: 307 LPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGA 486
L +DF RT+ RGLV APQ EILAH A FV+HCGWNS+LE L HG P++ WP+ +
Sbjct: 346 LMSDFTKRTEGRGLVITGWAPQLEILAHGATAFFVSHCGWNSLLEGLSHGKPILAWPMHS 525
Query: 487 EQHYNA 504
+Q +NA
Sbjct: 526 DQPWNA 543
Score = 68.0 bits (142), Expect(2) = 1e-032
Identities = 26/53 (49%), Positives = 32/53 (60%)
Frame = +1 / +1
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
Q EC+ WLD QPPSSV++LCFG+ Q E+A L RF+WVLR
Sbjct: 133 QPRHECMDWLDKQPPSSVLYLCFGTMSSLPGKQIEELAGALQSCEQRFIWVLR 291
>gb|CV058275.1|CV058275 BNEL36c3 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL36c3 5' similar to Zea mays
cis-zeatin O-glucosyltransferase 2, mRNA sequence
Length = 578
Score = 92.3 bits (195), Expect(2) = 1e-031
Identities = 37/66 (56%), Positives = 47/66 (71%)
Frame = +1 / +1
Query: 307 LPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGA 486
L +DF RT+ RGLV APQ EILAH A FV+HCGWNS+LE L HG P++ WP+ +
Sbjct: 346 LMSDFTKRTEGRGLVITGWAPQLEILAHGATAFFVSHCGWNSLLEGLSHGKPILAWPMHS 525
Query: 487 EQHYNA 504
+Q +NA
Sbjct: 526 DQPWNA 543
Score = 64.8 bits (135), Expect(2) = 1e-031
Identities = 25/53 (47%), Positives = 31/53 (58%)
Frame = +1 / +1
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
Q EC+ WLD QPPSS ++LCFG+ Q E+A L RF+WVLR
Sbjct: 133 QPRHECMDWLDKQPPSSGLYLCFGTMSSLPGKQIEELAGALQSCEQRFIWVLR 291
>gb|BF623637.2|BF623637 HVSMEa0003E04f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0003E04f, mRNA sequence
Length = 485
Score = 91.3 bits (193), Expect(2) = 3e-031
Identities = 38/74 (51%), Positives = 46/74 (62%)
Frame = +1 / +1
Query: 280 PSDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGV 459
P + E L F AR KDRG++ APQ IL H A+GGFVTHCGWNS +E + GV
Sbjct: 241 PKFPEVEEWLADGFEARVKDRGMILRGWAPQVMILWHQAIGGFVTHCGWNSTIEGICAGV 420
Query: 460 PMVPWPLGAEQHYN 501
PM+ WP +EQ N
Sbjct: 421 PMITWPHFSEQFVN 462
Score = 64.3 bits (134), Expect(2) = 3e-031
Identities = 24/59 (40%), Positives = 36/59 (61%)
Frame = +1 / +1
Query: 88 AEQQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLRGTPE 264
A +C++WLDS+ P SV+F+ FGS C T Q E+ GL+ S F+WV++ P+
Sbjct: 70 ASMDETQCLQWLDSRKPGSVIFVSFGSLACTTPQQLVELGLGLEASQKPFIWVIKAGPK 246
>gb|BM443110.2|BM443110 EBro02_SQ002_J16_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ002_J16 5', mRNA sequence
Length = 427
Score = 88.6 bits (187), Expect(2) = 2e-030
Identities = 35/66 (53%), Positives = 43/66 (65%)
Frame = +1 / +3
Query: 280 PSDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGV 459
P + E L F AR KDRG++ + APQ IL H A+GGFVTHCGWNS +E + GV
Sbjct: 228 PKFPEVEEWLADGFEARVKDRGMILRSWAPQVMILWHQAIGGFVTHCGWNSTIEGICAGV 407
Query: 460 PMVPWP 477
PM+ WP
Sbjct: 408 PMITWP 425
Score = 64.3 bits (134), Expect(2) = 2e-030
Identities = 24/59 (40%), Positives = 36/59 (61%)
Frame = +1 / +3
Query: 88 AEQQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLRGTPE 264
A +C++WLDS+ P SV+F+ FGS C T Q E+ GL+ S F+WV++ P+
Sbjct: 57 ASMDETQCLQWLDSRKPGSVIFVSFGSLACTTPQQLVELGLGLEASQKPFIWVIKAGPK 233
>gb|CA009114.1|CA009114 HU13D11r HU Hordeum vulgare subsp. vulgare cDNA clone HU13D11
5-PRIME, mRNA sequence
Length = 581
Score = 94.1 bits (199), Expect(2) = 7e-030
Identities = 40/72 (55%), Positives = 48/72 (66%)
Frame = +1 / +2
Query: 364 APQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVAL 543
APQ ILAH AVGGF+THCGW S LES+ GVPMV WPL AEQ N +V +GV V++
Sbjct: 290 APQVAILAHPAVGGFMTHCGWGSTLESVAAGVPMVTWPLFAEQFVNEKLIVDVLGVGVSV 469
Query: 544 NVERKRKNFVEA 579
V + N + A
Sbjct: 470 GVRKPTANVLTA 505
Score = 57.0 bits (118), Expect(2) = 7e-030
Identities = 22/61 (36%), Positives = 31/61 (50%)
Frame = +1 / +2
Query: 73 PSTPPAEQQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
PS P A+ C+ WLD + SVV++ FGS GC Q ++ L +WV+R
Sbjct: 23 PSLDPESDDARRCMSWLDGKKAESVVYVSFGSAGCIPPAQLMQLGMALVSCRWPVMWVMR 202
Query: 253 G 255
G
Sbjct: 203 G 205
>emb|AJ496572.1|HVU496572 Hordeum vulgare partial mRNA for putative glycosyltransferase (sf2
gene)
Length = 863
Score = 88.1 bits (186), Expect(2) = 1e-029
Identities = 38/72 (52%), Positives = 49/72 (68%)
Frame = +1 / +1
Query: 337 DRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLV 516
DRG + APQ IL+HAA+GGFVTHCGWNSVLE++ GVPMV WP A+Q N +V
Sbjct: 538 DRGFMVRGWAPQMLILSHAALGGFVTHCGWNSVLEAVSAGVPMVTWPRYADQFNNEKLVV 717
Query: 517 ADMGVAVALNVE 552
+ V V++ +
Sbjct: 718 ELLKVGVSIGAK 753
Score = 62.0 bits (129), Expect(2) = 1e-029
Identities = 23/60 (38%), Positives = 35/60 (58%)
Frame = +1 / +1
Query: 70 IPSTPPAEQQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVL 249
+ T A C+RWLD++P SVV++ FG+ F + H++A LD SG F+WV+
Sbjct: 289 VRGTDAPSPDADSCLRWLDAKPAGSVVYVSFGTLTKFAPAELHQLARALDLSGVNFVWVI 468
>gb|BF253477.2|BF253477 HVSMEf0001A18f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0001A18f, mRNA sequence
Length = 612
Score = 88.1 bits (186), Expect(2) = 2e-028
Identities = 38/73 (52%), Positives = 48/73 (65%)
Frame = +1 / +3
Query: 319 FLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHY 498
F R KDRGL+ APQ IL+H AVGGF+THCGWN+ LE++ HGVP + WP A+Q
Sbjct: 213 FEERVKDRGLLVRGWAPQVTILSHPAVGGFLTHCGWNATLEAISHGVPALTWPNFADQFC 392
Query: 499 NAFTLVADMGVAV 537
+ LV + V V
Sbjct: 393 SERLLVDVLRVGV 431
Score = 58.3 bits (121), Expect(2) = 2e-028
Identities = 22/51 (43%), Positives = 33/51 (64%)
Frame = +1 / +3
Query: 100 AQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
A V WLD++PP+SV+++ FGS A Q E+A GL+ SG F+W ++
Sbjct: 12 AGRIVSWLDARPPASVLYISFGSIAKLPAKQVAELARGLEASGRPFVWAIK 164
>gb|CA015634.1|CA015634 HT14O18r HT Hordeum vulgare subsp. vulgare cDNA clone HT14O18
5-PRIME, mRNA sequence
Length = 622
Score = 85.8 bits (181), Expect(2) = 4e-028
Identities = 37/72 (51%), Positives = 48/72 (66%)
Frame = +1 / +2
Query: 337 DRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLV 516
DRG + APQ IL+HAA+GGFVTHCGWNSVLE++ GVPMV WP A+Q N +V
Sbjct: 272 DRGFMVRGWAPQMLILSHAALGGFVTHCGWNSVLEAVSAGVPMVTWPRYADQFNNEKLVV 451
Query: 517 ADMGVAVALNVE 552
+ V ++ +
Sbjct: 452 ELLKVGFSIGAK 487
Score = 59.3 bits (123), Expect(2) = 4e-028
Identities = 21/47 (44%), Positives = 32/47 (68%)
Frame = +1 / +2
Query: 109 CVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVL 249
C+RWLD++P SVV++ FG+ F + H++A LD SG F+WV+
Sbjct: 62 CLRWLDAKPAGSVVYVSFGTLTKFAPAELHQLARALDLSGVNFVWVI 202
>gb|BF622731.2|BF622731 HVSMEa0008E02f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0008E02f, mRNA sequence
Length = 718
Score = 75.3 bits (158), Expect(3) = 2e-027
Identities = 29/65 (44%), Positives = 42/65 (64%)
Frame = +1 / +2
Query: 367 PQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVALN 546
PQ+E+L H AVG F+TH GWNS LESL+ GVP++ WP A+Q N + + G + ++
Sbjct: 482 PQQEVLNHPAVGAFLTHSGWNSALESLFGGVPVISWPFFADQQTNCRSQCNEWGRGMEID 661
Query: 547 VERKR 561
+R
Sbjct: 662 SNVRR 676
Score = 60.2 bits (125), Expect(3) = 2e-027
Identities = 23/53 (43%), Positives = 35/53 (66%)
Frame = +1 / +2
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
++ EC++WLD + P SVV++ FGS T Q E A GL +SG +F+W++R
Sbjct: 236 KEQDECLQWLDGRQPGSVVYVNFGSITVMTNAQMVEFAWGLAQSGKQFMWIVR 394
Score = 25.4 bits (49), Expect(3) = 2e-027
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +1 / +2
Query: 298 AELLPADFLARTKDRGLV 351
A +LP +FLA T RGL+
Sbjct: 416 AAVLPEEFLAETAGRGLM 469
>gb|BQ763397.1|BQ763397 EBro02_SQ008_B12_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_B12 5', mRNA sequence
Length = 552
Score = 122 bits (261), Expect = 3e-027
Identities = 53/88 (60%), Positives = 65/88 (73%)
Frame = +1 / +3
Query: 280 PSDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGV 459
P + +L +LLP FL RTK RGLV + APQ+++LAH AVG FVTHCGWNSVLES+ GV
Sbjct: 66 PPEPDLDDLLPECFLDRTKGRGLVVKSWAPQRDVLAHDAVGCFVTHCGWNSVLESVMAGV 245
Query: 460 PMVPWPLGAEQHYNAFTLVADMGVAVAL 543
PM+ WPL AEQ NA L +M +AVA+
Sbjct: 246 PMLAWPLYAEQRMNAVFLEKEMELAVAM 329
>gb|BF624531.2|BF624531 HVSMEa0017A18f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0017A18f, mRNA sequence
Length = 848
Score = 81.7 bits (172), Expect(2) = 5e-026
Identities = 34/82 (41%), Positives = 45/82 (54%)
Frame = +1 / +2
Query: 25 TRGVPAPPVYSIGPVIPSTPPAEQQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEI 204
+R AP + P+I + + C+ WLD QP SVVF+ FGSGG HE+
Sbjct: 122 SRSPAAPRCTTSAPIIRTDAAGHAPSAGCLDWLDRQPGKSVVFISFGSGGSLPTEHMHEL 301
Query: 205 AHGLDRSGHRFLWVLRGTPEPG 270
A GL+ SG RFLWV+R + G
Sbjct: 302 ALGLELSGQRFLWVVRSPSDEG 367
Score = 56.5 bits (117), Expect(2) = 5e-026
Identities = 22/41 (53%), Positives = 28/41 (68%)
Frame = +1 / +2
Query: 307 LPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWN 429
LPA F+ R+KD LV P+ AP+ E+L H A F+ HCGWN
Sbjct: 422 LPAGFVKRSKDARLVVPSWAPKTEVLVHDATVCFLMHCGWN 544
>gb|CA028126.1|CA028126 HZ61B05r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ61B05
5-PRIME, mRNA sequence
Length = 565
Score = 79.9 bits (168), Expect(2) = 2e-025
Identities = 32/47 (68%), Positives = 37/47 (78%)
Frame = +1 / +3
Query: 325 ARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPM 465
ART RGLV APQ +L+H +VGGFVTHCGWNSVLES+ HGVP+
Sbjct: 423 ARTSGRGLVVRGWAPQLAVLSHRSVGGFVTHCGWNSVLESIAHGVPV 563
Score = 56.5 bits (117), Expect(2) = 2e-025
Identities = 23/47 (48%), Positives = 28/47 (59%)
Frame = +1 / +3
Query: 112 VRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
V WLD Q P SVV++ FGS Q EI HGL+ G FLWV++
Sbjct: 231 VAWLDKQAPDSVVYVSFGSVARKLPKQQFEIGHGLEHCGRPFLWVVK 371
>gb|BM101456.2|BM101456 EBpi01_SQ003_O13_R pistil, 1 DPA, no treatment, cv Optic, EBpi01
Hordeum vulgare subsp. vulgare cDNA clone
EBpi01_SQ003_O13 5', mRNA sequence
Length = 355
Score = 67.0 bits (140), Expect(2) = 7e-024
Identities = 28/57 (49%), Positives = 36/57 (63%)
Frame = +1 / +1
Query: 319 FLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAE 489
F R RG+V PQ +LAH AVGGF+TH GW+S++ES G P+V PL A+
Sbjct: 184 FAERVAGRGVVRAGWVPQVRVLAHGAVGGFLTHAGWSSLMESFLFGHPLVMLPLFAD 354
Score = 63.8 bits (133), Expect(2) = 7e-024
Identities = 26/52 (50%), Positives = 32/52 (61%)
Frame = +1 / +1
Query: 112 VRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLRGTPEP 267
+RWLD QP SV+++ FGS TA E+A GL+ SG RFLW LR P
Sbjct: 10 MRWLDEQPARSVLYVAFGSEAPLTAENIRELAAGLELSGARFLWALREASAP 165
>gb|AV927070.1|AV927070 AV927070 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26k01 5', mRNA sequence
Length = 676
Score = 111 bits (236), Expect = 1e-023
Identities = 50/96 (52%), Positives = 63/96 (65%)
Frame = +1 / +3
Query: 256 TPEPGTKLPSDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSV 435
+P+ ++ + +L LLP FL RTK RGLV + APQ ++L H A G FVTHCGWNSV
Sbjct: 45 SPKKYLEVRPEPDLDALLPQGFLDRTKGRGLVVKSWAPQVDVLRHRATGAFVTHCGWNSV 224
Query: 436 LESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVAL 543
LE++ GVPM+ PL AEQ N + DMGVAV L
Sbjct: 225 LEAVAAGVPMLCLPLEAEQKMNKVCMTEDMGVAVEL 332
>gb|AV931900.1|AV931900 AV931900 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd26k01 3', mRNA sequence
Length = 631
Score = 111 bits (236), Expect = 1e-023
Identities = 50/96 (52%), Positives = 63/96 (65%)
Frame = +1 / -1
Query: 256 TPEPGTKLPSDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSV 435
+P+ ++ + +L LLP FL RTK RGLV + APQ ++L H A G FVTHCGWNSV
Sbjct: 598 SPKKYLEVRPEPDLDALLPQGFLDRTKGRGLVVKSWAPQVDVLRHRATGAFVTHCGWNSV 419
Query: 436 LESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVAL 543
LE++ GVPM+ PL AEQ N + DMGVAV L
Sbjct: 418 LEAVAAGVPMLCLPLEAEQKMNKVCMTEDMGVAVEL 311
>gb|AV929760.1|AV929760 AV929760 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd16h10 3', mRNA sequence
Length = 669
Score = 73.5 bits (154), Expect(2) = 1e-023
Identities = 27/65 (41%), Positives = 40/65 (61%)
Frame = +1 / -3
Query: 364 APQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVAL 543
APQ+ +L+H +V FV+HCGWNS +E L HGVP + WP A+Q N + G V +
Sbjct: 352 APQQRVLSHPSVACFVSHCGWNSTMEGLRHGVPFLCWPYFADQFCNQSYICNVWGTGVKI 173
Query: 544 NVERK 558
+ + +
Sbjct: 172 HADER 158
Score = 56.5 bits (117), Expect(2) = 1e-023
Identities = 22/53 (41%), Positives = 33/53 (62%)
Frame = +1 / -3
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
Q+ C+ WLD+Q SV+++ FGS F A + E+A GL+ +G FLW +R
Sbjct: 595 QEDSACLPWLDAQARGSVIYVAFGSFTVFDAARFLELADGLELTGRPFLWTVR 437
>gb|AV927784.1|AV927784 AV927784 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3k12 3', mRNA sequence
Length = 651
Score = 73.5 bits (154), Expect(2) = 1e-023
Identities = 27/65 (41%), Positives = 40/65 (61%)
Frame = +1 / -1
Query: 364 APQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVAL 543
APQ+ +L+H +V FV+HCGWNS +E L HGVP + WP A+Q N + G V +
Sbjct: 339 APQQRVLSHPSVACFVSHCGWNSTMEGLRHGVPFLCWPYFADQFCNQSYICNVWGTGVKI 160
Query: 544 NVERK 558
+ + +
Sbjct: 159 HADER 145
Score = 56.5 bits (117), Expect(2) = 1e-023
Identities = 22/53 (41%), Positives = 33/53 (62%)
Frame = +1 / -1
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
Q+ C+ WLD+Q SV+++ FGS F A + E+A GL+ +G FLW +R
Sbjct: 582 QEDSACLPWLDAQARGSVIYVAFGSFTVFDAARFLELADGLELTGRPFLWTVR 424
>gb|BM816260.1|BM816260 HC113E04_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113E04_SK.ab1 similar to
Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase, mRNA sequence
Length = 842
Score = 71.6 bits (150), Expect(2) = 2e-022
Identities = 29/68 (42%), Positives = 39/68 (57%)
Frame = +1 / +2
Query: 358 TKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAV 537
T PQ+++L H A+G F+TH GWNS LE + GVPMV WP EQ N + G+ +
Sbjct: 293 TWCPQEKVLEHEAIGVFLTHSGWNSSLEGICGGVPMVCWPFFTEQQTNCRYKCTEWGIGM 472
Query: 538 ALNVERKR 561
+ E R
Sbjct: 473 EIGEEVTR 496
Score = 54.7 bits (113), Expect(2) = 2e-022
Identities = 23/47 (48%), Positives = 30/47 (63%)
Frame = +1 / +2
Query: 112 VRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
+RWLD+QPP SVV++ FGS + E A GL SG+ FLW +R
Sbjct: 71 LRWLDNQPPGSVVYVNFGSITVMSNEHLLEFAWGLANSGYTFLWNVR 211
>gb|BM816841.1|BM816841 HB105A11_SK.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB105A11_SK.ab1 similar to
Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase, mRNA sequence
Length = 872
Score = 74.4 bits (156), Expect(2) = 4e-022
Identities = 28/64 (43%), Positives = 41/64 (64%)
Frame = +1 / +3
Query: 370 QKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVALNV 549
Q+ ++ H AVG F+THCGWNS++E L GVPM+ WP AEQ N+ + GV + +
Sbjct: 486 QEAVMRHEAVGAFLTHCGWNSMMEGLGAGVPMLCWPFFAEQQTNSRYACVEWGVGMEVGD 665
Query: 550 ERKR 561
+ +R
Sbjct: 666 DVRR 677
Score = 50.6 bits (104), Expect(2) = 4e-022
Identities = 19/53 (35%), Positives = 31/53 (58%)
Frame = +1 / +3
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
++ Q C+ WL + P SVV++ +GS + + E A GL G+ FLW++R
Sbjct: 237 REDQSCLEWLQGREPRSVVYVNYGSVTTMSKQELVEFAWGLANCGYDFLWIVR 395
>gb|BF256192.2|BF256192 HVSMEf0009B02f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0009B02f, mRNA sequence
Length = 782
Score = 105 bits (224), Expect = 4e-022
Identities = 42/60 (70%), Positives = 49/60 (81%)
Frame = +1 / +2
Query: 322 LARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYN 501
L RTKDRGLV + APQ+E+LAH +VGGFVTHCGWNSVLE++ GVPM+ WPL AEQ N
Sbjct: 2 LDRTKDRGLVVMSWAPQREVLAHGSVGGFVTHCGWNSVLEAVMAGVPMLGWPLYAEQRMN 181
>gb|BI953418.1|BI953418 HVSMEm0012J18f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0012J18f, mRNA sequence
Length = 855
Score = 81.7 bits (172), Expect(2) = 1e-021
Identities = 31/74 (41%), Positives = 48/74 (64%)
Frame = +1 / +1
Query: 283 SDGNLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVP 462
++G L ++F +T+ GLV APQ EILAH A F++HCGWNS +ES+ HG P
Sbjct: 163 AEGRRHARLASEFAEQTRGTGLVITGWAPQLEILAHPATAAFMSHCGWNSTVESMSHGKP 342
Query: 463 MVPWPLGAEQHYNA 504
++ WP+ ++ ++A
Sbjct: 343 ILAWPMHSDHPWDA 384
Score = 41.4 bits (84), Expect(2) = 1e-021
Identities = 18/43 (41%), Positives = 24/43 (55%)
Frame = +1 / +1
Query: 124 DSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
D Q +SV+++ FGS Q E+A L S RF+WVLR
Sbjct: 1 DEQAAASVLYVSFGSTSSLRGGQVAELAAALKGSKQRFIWVLR 129
>gb|BF622661.2|BF622661 HVSMEa0007K11f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0007K11f, mRNA sequence
Length = 697
Score = 68.0 bits (142), Expect(3) = 3e-021
Identities = 26/42 (61%), Positives = 32/42 (76%)
Frame = +1 / +2
Query: 367 PQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQ 492
PQ E+LAH A+G F+THCGWNS LE+L +GVP V P A+Q
Sbjct: 251 PQLEVLAHRAIGCFITHCGWNSTLEALVNGVPFVGIPHWADQ 376
Score = 52.8 bits (109), Expect(3) = 3e-021
Identities = 22/53 (41%), Positives = 31/53 (58%)
Frame = +1 / +2
Query: 106 ECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLRGTPE 264
EC+ WL+ Q SSVV + +G+ + A Q E+ +GL S FLWV+R E
Sbjct: 29 ECMDWLEIQMNSSVVLVSYGTVSNYDATQLEELGNGLCNSSKPFLWVVRSNEE 187
Score = 23.1 bits (44), Expect(3) = 3e-021
Identities = 10/27 (37%), Positives = 14/27 (51%)
Frame = +1 / +2
Query: 616 DGETARKVRDKVMEIKAACRKAMEEGG 696
DGE K + M ++AM+EGG
Sbjct: 491 DGERKDKYKKNAMNWMQKGQEAMQEGG 571
>gb|BQ466592.1|BQ466592 HT02P16r HT Hordeum vulgare subsp. vulgare cDNA clone HT02P16
5-PRIME, mRNA sequence
Length = 606
Score = 65.7 bits (137), Expect(2) = 3e-021
Identities = 21/38 (55%), Positives = 29/38 (76%)
Frame = +1 / +1
Query: 364 APQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWP 477
APQ+ +L+H ++ FV+HCGWNS +E L HGVP + WP
Sbjct: 487 APQQRVLSHPSIACFVSHCGWNSTMEGLRHGVPFLCWP 600
Score = 56.5 bits (117), Expect(2) = 3e-021
Identities = 22/48 (45%), Positives = 31/48 (64%)
Frame = +1 / +1
Query: 109 CVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
C+ WLD+Q SVV++ FGS F A + E+A GL+ +G FLW +R
Sbjct: 259 CLPWLDAQARGSVVYVAFGSFTIFDAARFQELADGLELTGRPFLWAVR 402
>gb|AJ462599.1|AJ462599 AJ462599 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200036E05F1, mRNA sequence
Length = 360
Score = 102 bits (218), Expect = 3e-021
Identities = 42/84 (50%), Positives = 55/84 (65%)
Frame = +1 / +1
Query: 292 NLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVP 471
+L L+P FL RTKDRGLV + APQ ++L H A G F+THCGWNS LE + G+P++
Sbjct: 37 DLDALMPEGFLERTKDRGLVVKSWAPQVDVLRHRATGAFMTHCGWNSTLEGITAGLPLLC 216
Query: 472 WPLGAEQHYNAFTLVADMGVAVAL 543
WPL AEQ N +V M + V +
Sbjct: 217 WPLYAEQRVNKVHIVDGMKLGVEM 288
>gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0012H19f, mRNA sequence
Length = 847
Score = 61.6 bits (128), Expect(2) = 4e-021
Identities = 25/69 (36%), Positives = 40/69 (57%)
Frame = +1 / +3
Query: 46 PVYSIGPVIPSTPPAEQQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRS 225
P++ P + +Q + C++WLD+QP +SV+++ FGS C +A E A G+ S
Sbjct: 105 PLHLFSPAAAAESSLLRQDRSCLKWLDAQPAASVLYVSFGSLACMSARDLVETAWGIAGS 284
Query: 226 GHRFLWVLR 252
FLWV+R
Sbjct: 285 RVPFLWVVR 311
Score = 60.2 bits (125), Expect(2) = 4e-021
Identities = 26/47 (55%), Positives = 31/47 (65%)
Frame = +1 / +3
Query: 364 APQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNA 504
APQ+E+L H AV GF TH GWNS ES+ GVPM+ P +Q NA
Sbjct: 399 APQEEVLRHPAVAGFWTHGGWNSTTESVCEGVPMLCRPHLXDQMGNA 539
>gb|BF628779.2|BF628779 HVSMEb0008B22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0008B22f, mRNA sequence
Length = 741
Score = 63.4 bits (132), Expect(2) = 4e-021
Identities = 28/77 (36%), Positives = 43/77 (55%)
Frame = +1 / +3
Query: 370 QKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAVALNV 549
Q+ +L H A+G F+THCGWNS ++ G+PM+ WP AEQ N+ D V L V
Sbjct: 423 QEAVLQHPALGVFLTHCGWNSTFVAISAGLPMLCWPFFAEQQTNSRYACLDDEWGVRLGV 602
Query: 550 ERKRKNFVEATELERAV 600
+ + V +++ A+
Sbjct: 603 GDEVRREVVESKIRAAM 653
Score = 58.3 bits (121), Expect(2) = 4e-021
Identities = 22/53 (41%), Positives = 33/53 (62%)
Frame = +1 / +3
Query: 94 QQAQECVRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
++ + C+ WLD + P SVV++ FGS + + E A GL SGH FLW++R
Sbjct: 159 REDRSCLEWLDGREPGSVVYVNFGSITTMSGEELVEFAWGLANSGHPFLWIVR 317
>gb|AV833284.1|AV833284 AV833284 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags5b03, mRNA sequence
Length = 671
Score = 69.8 bits (146), Expect(2) = 4e-021
Identities = 29/68 (42%), Positives = 40/68 (58%)
Frame = +1 / +1
Query: 358 TKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAV 537
T PQ+++L AVG F+TH GWNS LE + GVPMV WP A+Q N + G+ +
Sbjct: 325 TWCPQEKVLEQEAVGVFLTHSGWNSSLEGICGGVPMVCWPFFADQQTNCRYKCTEWGIGM 504
Query: 538 ALNVERKR 561
+ + KR
Sbjct: 505 EIGDDVKR 528
Score = 51.9 bits (107), Expect(2) = 4e-021
Identities = 21/47 (44%), Positives = 29/47 (61%)
Frame = +1 / +1
Query: 112 VRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
+RWLD +PP SVV++ FGS + E A GL +G+ FLW +R
Sbjct: 103 LRWLDGRPPRSVVYVNFGSIAVMSKEHMLEFAWGLANTGYAFLWNVR 243
>gb|BJ463253.1|BJ463253 BJ463253 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26i01 5', mRNA sequence
Length = 518
Score = 69.8 bits (146), Expect(2) = 4e-021
Identities = 29/68 (42%), Positives = 40/68 (58%)
Frame = +1 / +3
Query: 358 TKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVPWPLGAEQHYNAFTLVADMGVAV 537
T PQ+++L AVG F+TH GWNS LE + GVPMV WP A+Q N + G+ +
Sbjct: 288 TWCPQEKVLEQEAVGVFLTHSGWNSSLEGICGGVPMVCWPFFADQQTNCRYKCTEWGIGM 467
Query: 538 ALNVERKR 561
+ + KR
Sbjct: 468 EIGDDVKR 491
Score = 51.9 bits (107), Expect(2) = 4e-021
Identities = 21/47 (44%), Positives = 29/47 (61%)
Frame = +1 / +3
Query: 112 VRWLDSQPPSSVVFLCFGSGGCFTAPQAHEIAHGLDRSGHRFLWVLR 252
+RWLD +PP SVV++ FGS + E A GL +G+ FLW +R
Sbjct: 66 LRWLDGRPPRSVVYVNFGSIAVMSKEHMLEFAWGLANTGYAFLWNVR 206
>gb|BJ450621.1|BJ450621 BJ450621 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30g17 5', mRNA sequence
Length = 616
Score = 101 bits (215), Expect = 7e-021
Identities = 42/84 (50%), Positives = 54/84 (64%)
Frame = +1 / +1
Query: 292 NLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVP 471
+L L+P FL RTK RGLV + APQ E+L H A G F+THCGWNS LE + G+P++
Sbjct: 31 DLDALMPEGFLERTKGRGLVVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPLLC 210
Query: 472 WPLGAEQHYNAFTLVADMGVAVAL 543
WPL AEQ N +V M + V +
Sbjct: 211 WPLYAEQRVNKVHIVEGMKLGVEM 282
>gb|BJ458043.1|BJ458043 BJ458043 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak30g17 3', mRNA sequence
Length = 551
Score = 101 bits (215), Expect = 7e-021
Identities = 42/84 (50%), Positives = 54/84 (64%)
Frame = +1 / -3
Query: 292 NLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVP 471
+L L+P FL RTK RGLV + APQ E+L H A G F+THCGWNS LE + G+P++
Sbjct: 543 DLDALMPEGFLERTKGRGLVVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPLLC 364
Query: 472 WPLGAEQHYNAFTLVADMGVAVAL 543
WPL AEQ N +V M + V +
Sbjct: 363 WPLYAEQRVNKVHIVEGMKLGVEM 292
>gb|CA001229.1|CA001229 HS18N07u HS Hordeum vulgare subsp. vulgare cDNA clone HS18N07
3-PRIME, mRNA sequence
Length = 544
Score = 101 bits (215), Expect = 7e-021
Identities = 42/84 (50%), Positives = 54/84 (64%)
Frame = +1 / -2
Query: 292 NLAELLPADFLARTKDRGLVWPTKAPQKEILAHAAVGGFVTHCGWNSVLESLWHGVPMVP 471
+L L+P FL RTK RGLV + APQ E+L H A G F+THCGWNS LE + G+P++
Sbjct: 489 DLDALMPEGFLERTKGRGLVVKSWAPQVEVLRHRATGAFMTHCGWNSTLEGITAGLPLLC 310
Query: 472 WPLGAEQHYNAFTLVADMGVAVAL 543
WPL AEQ N +V M + V +
Sbjct: 309 WPLYAEQRVNKVHIVEGMKLGVEM 238
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 621,221,753
Number of Sequences: 312970
Number of extensions: 13450951
Number of successful extensions: 234839
Number of sequences better than 1.0e-020: 51
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 12673225439
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)