TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2478343.2.1
(694 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subs... 112 7e-026
>gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200058E12F1, mRNA sequence
Length = 360
Score = 112 bits (239), Expect(2) = 7e-026
Identities = 43/58 (74%), Positives = 47/58 (81%)
Frame = -2 / +2
Query: 693 EVLAHPAVGGFFTHCGWNSTVEAVSEGVPMICHPRHGDQYGNARYVCHVWKVGTEVAG 520
EVL HPAV GF+TH GWNST E++ EGVPM+C P GDQ GNARYV HVWKVG EVAG
Sbjct: 44 EVLRHPAVAGFWTHGGWNSTTESMCEGVPMLCRPHFGDQMGNARYVEHVWKVGFEVAG 217
Score = 24.4 bits (47), Expect(2) = 7e-026
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = -2 / +2
Query: 513 LERGEIKAAIDRLMGGSEEGE 451
LER +++ AI RL+ GSE E
Sbjct: 221 LERLDVEKAIRRLVTGSEGAE 283
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 255,720,431
Number of Sequences: 312970
Number of extensions: 3636645
Number of successful extensions: 70090
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7592590911
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)