TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448637.2.1
(1190 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA li... 312 3e-084
gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. v... 298 3e-080
gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Li... 253 8e-079
gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA li... 226 3e-072
gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. v... 270 8e-072
gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedl... 237 7e-062
gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, n... 177 1e-047
gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-a... 138 2e-042
gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Le... 170 1e-041
gb|BF254204.2|BF254204 HVSMEf0003E24f Hordeum vulgare seedl... 77 7e-039
gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA li... 157 9e-038
gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA li... 150 1e-035
gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subs... 111 5e-028
gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiol... 85 2e-027
gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. v... 105 4e-026
gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachi... 109 3e-023
>gb|BJ459891.1|BJ459891 BJ459891 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak38k12 3', mRNA sequence
Length = 659
Score = 312 bits (675), Expect = 3e-084
Identities = 118/154 (76%), Positives = 140/154 (90%)
Frame = -3 / -2
Query: 837 GFPEAATLARWVCQSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFKEKPMFTFA 658
GFPEAATLARW+CQSY +HLG+EL Q+D AGE+LL+MLW H DA+LCCSFKEKP+FTFA
Sbjct: 658 GFPEAATLARWICQSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFA 479
Query: 657 NKAGLDMLETSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHV 478
N+ G++MLETS VALQDL+LDKIFDE+GRKAL+S+I KLMEQG+ YLP GVC+SGMGRHV
Sbjct: 478 NEMGINMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHV 299
Query: 477 SFDQAVAWKVLGEDSNIHCLAFCFVNWSFV*LKS 376
SF+ A+AWKV+GED+N+HCLAFCFVNWSFV* S
Sbjct: 298 SFENAIAWKVVGEDNNVHCLAFCFVNWSFV*SSS 197
Score = 231 bits (499), Expect = 6e-060
Identities = 106/151 (70%), Positives = 114/151 (75%)
Frame = +2 / +3
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENS 565
HTKDQLTKQ ARQ LLSSP+TFHA A+SKETWRPIPD HTP GR PCSI+F +SE S
Sbjct: 207 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 386
Query: 566 AFLPDSSKILSSVRSCSATRDVSNMSSPALFANVNIGFSLKEQHRTASG*CQSIFNNASP 745
AFLP SS ILS+ RS AT+DVSNM P FANVN GFSLKEQH+ AS * QSI N SP
Sbjct: 387 AFLPASSNILSNERS*RATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSP 566
Query: 746 APSD*LSNSIPRW***LWQTHLASVAASGKP 838
A S L+NS PRW *LW ASVAASGKP
Sbjct: 567 ASSACLNNSTPRWNR*LWHIQRASVAASGKP 659
Score = 149 bits (319), Expect = 4e-035
Identities = 73/151 (48%), Positives = 94/151 (62%)
Frame = +1 / +2
Query: 385 SHEGPVDEAERQAVDVAILAEHLPRYSLVERNMXXXXXXXXXXRQVRVALFH*FRDV*E* 564
SHE PVDEAE +AV + +L ++ P YS++E +M QV AL H D+ *
Sbjct: 206 SHERPVDEAEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV* 385
Query: 565 CFPS*LVEDLV*RQVLQRNKRCF*HVQPCLVCKRKHRFLLKGAAQNSIWMMPEHFQQCFT 744
C + ++ LV R+VL+ N+RCF HV P LVCKRK+R L+ AQNSI+M+PEH Q F
Sbjct: 386 CLSTSFIKYLVQREVLKGNERCFQHVNPHLVCKRKYRLFLERTAQNSIFMIPEHSQ*RFP 565
Query: 745 SSI*LIK*FHT*MVVITLANPSSKCGSFREA 837
S I L K* M + LA P+SKC SFREA
Sbjct: 566 SFICLSK*LDPQMEPVALAYPASKCCSFREA 658
Score = 104 bits (221), Expect = 1e-021
Identities = 60/139 (43%), Positives = 75/139 (53%)
Frame = +3 / +1
Query: 420 GSGCCYPRRAPSTLQLGRKKHGGPFLTCTRLTAGTRSPVPLVSRCLRIVLSFLTRRRSCL 599
GS C P R S L R++HGGP T T AG +SP P IV + + SC
Sbjct: 241 GSAHCCPHRLLSML*HSRRRHGGPSQTSTHHLAGKQSPAPSTLGSQSIVPFYQLHQISCP 420
Query: 600 ASGLAAQQEMFLTCPALPCLQT*T*VSP*RSSTEQHLDDARAFSTMLHQLHLID*VIPYL 779
GL Q++MF TC + LQT* P*++ST+ HL D RAFS + QLHL *+
Sbjct: 421 TRGLEGQRKMFPTC*SPSRLQT*IQAFP*KNSTK*HLHDPRAFSIKIPQLHLPV*ITRPP 600
Query: 780 DGSDNSGKPI*QVWQLQGS 836
DG+ + G QV QLQGS
Sbjct: 601 DGTGSFGISSEQVLQLQGS 657
Score = 80.8 bits (170), Expect(2) = 1e-025
Identities = 39/70 (55%), Positives = 49/70 (70%)
Frame = -1 / -3
Query: 836 ASLKLPHLLDGFARVITTI*VWNYLINQMELVKHC*KCSGIIQMLFCAAPLRRNLCLRLQ 657
ASLKL HLL G+A+ +I +YL QM+L *+CSGI++MLFCA R++L LRLQ
Sbjct: 657 ASLKLQHLLAGYAKATGSIWGSSYLDRQMKLGNLY*ECSGIMKMLFCAVLSRKSLYLRLQ 478
Query: 656 TRQGWTC*KH 627
TR G TC KH
Sbjct: 477 TRWGLTCWKH 448
Score = 56.1 bits (116), Expect(3) = 3e-022
Identities = 26/65 (40%), Positives = 33/65 (50%)
Frame = -1 / -3
Query: 569 KHYSQTSRN*WNRATRTCRQACACQEWAAMFLSTKL*RGRCXXXXXXXXXXXXXXXTGPS 390
+HY+ S++*W+RA TC+ CAC WAAM S L* G+ TG S
Sbjct: 390 RHYTLRSQS*WSRALFTCQVVCACLGWAAMSPSRML*HGK*SVRTTMCTALPSASSTGLS 211
Query: 389 CD*SP 375
CD P
Sbjct: 210 CDHPP 196
Score = 56.1 bits (116), Expect(2) = 1e-025
Identities = 30/62 (48%), Positives = 38/62 (61%)
Frame = -2 / -1
Query: 571 ESTILRHLETNGTGLRVPAVRRVHVRNGPPCFFRPSCSVEGARRG*QHPLPGVLLRQLVL 392
+ TIL + +G GL +PA V V +GPPC R S+E +R G Q LP +LLRQLV
Sbjct: 392 KGTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPCLLLRQLVF 213
Query: 391 RV 386
RV
Sbjct: 212 RV 207
Score = 50.6 bits (104), Expect(3) = 3e-022
Identities = 23/49 (46%), Positives = 29/49 (59%)
Frame = -2 / -1
Query: 733 VENALASSRCCSVLLL*GETYVYVCKQGRAGHVRNISCCAARPDARQDL 587
+ENAL S RC VL G+ +YVCK+ HV NI C +RP QD+
Sbjct: 554 IENALGS*RCYFVLFFQGKACIYVCKRDGD*HVGNIFRCPSRPLVGQDI 408
Score = 36.3 bits (73), Expect(3) = 3e-022
Identities = 12/20 (60%), Positives = 17/20 (85%)
Frame = -2 / -1
Query: 838 WLP*SCHTC*MGLPELSLPS 779
WLP*SC+TC + +P+L +PS
Sbjct: 659 WLP*SCNTCSLDMPKLPVPS 600
>gb|CB858305.1|CB858305 HI06I11w HI Hordeum vulgare subsp. vulgare cDNA clone HI06I11
3-PRIME, mRNA sequence
Length = 638
Score = 298 bits (646), Expect = 3e-080
Identities = 113/150 (75%), Positives = 135/150 (90%)
Frame = -3 / -1
Query: 825 AATLARWVCQSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFKEKPMFTFANKAG 646
AATLARW+C SY +HLG+EL Q+D AGE+LL+MLW H DA+LCCSFKEKP+FTFAN+ G
Sbjct: 638 AATLARWICHSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFANEMG 459
Query: 645 LDMLETSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQ 466
++MLETS VALQDL+LDKIFDE+GRKAL+S+I KLMEQG+ YLP GVC+SGMGRHVSF+
Sbjct: 458 INMLETSFVALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFEN 279
Query: 465 AVAWKVLGEDSNIHCLAFCFVNWSFV*LKS 376
A+AWKV+GED+N+HCLAFCFVNWSFV* S
Sbjct: 278 AIAWKVVGEDNNVHCLAFCFVNWSFV*SSS 189
Score = 219 bits (473), Expect = 2e-056
Identities = 101/146 (69%), Positives = 109/146 (74%)
Frame = +2 / +1
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENS 565
HTKDQLTKQ ARQ LLSSP+TFHA A+SKETWRPIPD HTP GR PCSI+F +SE S
Sbjct: 199 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 378
Query: 566 AFLPDSSKILSSVRSCSATRDVSNMSSPALFANVNIGFSLKEQHRTASG*CQSIFNNASP 745
AFLP SS ILS+ RS AT+DVSNM P FANVN GFSLKEQH+ AS * QSI N SP
Sbjct: 379 AFLPASSNILSNERS*RATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSP 558
Query: 746 APSD*LSNSIPRW***LWQTHLASVA 823
A S L+NS PRW *LW ASVA
Sbjct: 559 ASSACLNNSTPRWNR*LWHIQRASVA 636
Score = 138 bits (296), Expect = 6e-032
Identities = 68/147 (46%), Positives = 90/147 (61%)
Frame = +1 / +3
Query: 385 SHEGPVDEAERQAVDVAILAEHLPRYSLVERNMXXXXXXXXXXRQVRVALFH*FRDV*E* 564
SHE PVDEAE +AV + +L ++ P YS++E +M QV AL H D+ *
Sbjct: 198 SHERPVDEAEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV* 377
Query: 565 CFPS*LVEDLV*RQVLQRNKRCF*HVQPCLVCKRKHRFLLKGAAQNSIWMMPEHFQQCFT 744
C + ++ LV R+VL+ N+RCF HV P LVCKRK+R L+ AQNSI+M+PEH Q F
Sbjct: 378 CLSTSFIKYLVQREVLKGNERCFQHVNPHLVCKRKYRLFLERTAQNSIFMIPEHSQ*RFP 557
Query: 745 SSI*LIK*FHT*MVVITLANPSSKCGS 825
S I L K* M + +A P+SKC S
Sbjct: 558 SFICLSK*LDPQMEPVAVAYPASKCCS 638
Score = 71.6 bits (150), Expect(2) = 7e-023
Identities = 35/66 (53%), Positives = 44/66 (66%)
Frame = -1 / -2
Query: 824 LPHLLDGFARVITTI*VWNYLINQMELVKHC*KCSGIIQMLFCAAPLRRNLCLRLQTRQG 645
L HLL G+A +I +YL QM+L *+CSGI++MLFCA R++L LRLQTR G
Sbjct: 637 LQHLLAGYATATGSIWGSSYLDRQMKLGNLY*ECSGIMKMLFCAVLSRKSLYLRLQTRWG 458
Query: 644 WTC*KH 627
TC KH
Sbjct: 457 LTCWKH 440
Score = 56.1 bits (116), Expect(2) = 7e-023
Identities = 30/62 (48%), Positives = 38/62 (61%)
Frame = -2 / -3
Query: 571 ESTILRHLETNGTGLRVPAVRRVHVRNGPPCFFRPSCSVEGARRG*QHPLPGVLLRQLVL 392
+ TIL + +G GL +PA V V +GPPC R S+E +R G Q LP +LLRQLV
Sbjct: 384 KGTIL*DPKVDGAGLCLPARWCVLVWDGPPCLLRECYSMESSR*GQQCALPCLLLRQLVF 205
Query: 391 RV 386
RV
Sbjct: 204 RV 199
>gb|BE421582.1|BE421582 HWM011cD.08r ITEC HWM Barley Leaf Library Hordeum vulgare subsp.
vulgare cDNA clone HWM011cD.08, mRNA sequence
Length = 1501
Score = 253 bits (547), Expect(2) = 8e-079
Identities = 103/173 (59%), Positives = 135/173 (78%)
Frame = -3 / +2
Query: 1005 SVLTIAFQFPYEMHLQDSVAAMARQYVRSVISAVQRVSMAISPSQSGLNAGHRMLSGFPE 826
SVLTIAFQF YE HL++SVAAMARQYVR+V+++VQRV+MAI+PS+ G + G PE
Sbjct: 128 SVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSRPGGQIEMKNPPGSPE 307
Query: 825 AATLARWVCQSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFKEKPMFTFANKAG 646
A TLARW+ +SY +H G ELL + +A LK LW H D+++CCS K P+FTFAN+AG
Sbjct: 308 AHTLARWIGRSYRFHTGAELLCTESQSADASLKALWQHSDSIMCCSLKAAPVFTFANQAG 487
Query: 645 LDMLETSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMG 487
LDMLET+L+ALQD++L+KI D+ GRKAL S+ +M+QG+AYLP GVC+S +G
Sbjct: 488 LDMLETTLIALQDISLEKILDDDGRKALCSEFPXIMQQGFAYLPXGVCVSSIG 646
Score = 89.5 bits (189), Expect(3) = 1e-040
Identities = 43/85 (50%), Positives = 51/85 (60%)
Frame = +2 / -2
Query: 488 PIPDMHTPDGRYA*PCSISFEMSENSAFLPDSSKILSSVRSCSATRDVSNMSSPALFANV 667
P+ D HTP GRYA PC + SE+SA P SS+I SS S SA VS+MS PA A V
Sbjct: 645 PMLDTHTPXGRYAKPCCMIXGNSEHSALRPSSSRIFSSEMSWSAISVVSSMSRPAWLAKV 466
Query: 668 NIGFSLKEQHRTASG*CQSIFNNAS 742
N G + +EQH S C S F +AS
Sbjct: 465 NTGAAFREQHMIESECCHSAFRDAS 391
Score = 70.7 bits (148), Expect(3) = 1e-040
Identities = 40/72 (55%), Positives = 43/72 (59%)
Frame = +2 / -2
Query: 791 *LWQTHLASVAASGKPESILCPAFRPDWEGDIAIDTLCTAEMTLRTY*RAIAATLS*RCI 970
*L HLASV ASG P P EG IA+ TLCT T+ TY AIAATLS R
Sbjct: 342 *LLPIHLASV*ASGDPGGFFISICPPGREGAIAMATLCTDATTVLTYCLAIAATLSRRWF 163
Query: 971 SYGN*KAIVSTD 1006
SY N KA+VSTD
Sbjct: 162 SYENWKAMVSTD 127
Score = 61.6 bits (128), Expect(2) = 8e-079
Identities = 25/35 (71%), Positives = 28/35 (80%)
Frame = -3 / +3
Query: 489 GRHVSFDQAVAWKVLGEDSNIHCLAFCFVNWSFV* 385
GR VS++QAVAWKVL +D HCLAF VNWSFV*
Sbjct: 645 GRPVSYEQAVAWKVLXDDDAPHCLAFHVVNWSFV* 749
Score = 45.1 bits (92), Expect(3) = 1e-040
Identities = 22/35 (62%), Positives = 24/35 (68%)
Frame = +2 / -1
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRP 490
+TKDQLT ARQ SS STFHATA S +T RP
Sbjct: 748 YTKDQLTT*KARQCGASSSXSTFHATACSYDTGRP 644
>gb|BJ452342.1|BJ452342 BJ452342 K. Sato unpublished cDNA library, cv. Akashinriki vegetative
stage leaves Hordeum vulgare subsp. vulgare cDNA clone
baak38k12 5', mRNA sequence
Length = 633
Score = 226 bits (489), Expect(2) = 3e-072
Identities = 89/111 (80%), Positives = 102/111 (91%)
Frame = -3 / +1
Query: 1023 DDCNLRSVLTIAFQFPYEMHLQDSVAAMARQYVRSVISAVQRVSMAISPSQSGLNAGHRM 844
DDCNLRSVLTIAFQFPYEMHLQDSVA MARQYVRS++SAVQRVSMAISPS+SGLNA ++
Sbjct: 301 DDCNLRSVLTIAFQFPYEMHLQDSVATMARQYVRSIVSAVQRVSMAISPSRSGLNAEQKI 480
Query: 843 LSGFPEAATLARWVCQSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCC 691
+SGFPEAATLARW+CQSY +HLG+EL Q+D AGE+LL+MLW H DA+LCC
Sbjct: 481 ISGFPEAATLARWICQSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCC 633
Score = 158 bits (340), Expect(2) = 1e-050
Identities = 77/111 (69%), Positives = 83/111 (74%)
Frame = +2 / -2
Query: 692 QHRTASG*CQSIFNNASPAPSD*LSNSIPRW***LWQTHLASVAASGKPESILCPAFRPD 871
QH+ AS * QSI N SPA S L+NS PRW *LW ASVAASGKPE I C AF+PD
Sbjct: 632 QHKIASS*SQSILNKDSPASSACLNNSTPRWNR*LWHIQRASVAASGKPEIIFCSAFKPD 453
Query: 872 WEGDIAIDTLCTAEMTLRTY*RAIAATLS*RCISYGN*KAIVSTDLKLQSS 1024
EG+IAIDTL TAE LRTY RA+ ATLS RCIS GN*KAIVSTD +LQSS
Sbjct: 452 REGEIAIDTL*TAETMLRTYCRAMVATLSWRCIS*GN*KAIVSTDRRLQSS 300
Score = 133 bits (285), Expect(2) = 3e-039
Identities = 61/110 (55%), Positives = 78/110 (70%)
Frame = +1 / -1
Query: 694 AQNSIWMMPEHFQQCFTSSI*LIK*FHT*MVVITLANPSSKCGSFREARKHPMPSI*TRL 873
AQNSI+M+PEH Q F S I L K* M + LA P+SKC SFREAR + + SI TR
Sbjct: 630 AQNSIFMIPEHSQ*RFPSFICLSK*LDPQMEPVALAYPASKCCSFREARNYLLFSIQTRS 451
Query: 874 GGRYSHRHSLHSRNDTTNILTGHCCNAVLKVHLIRELKGDCQHRSQIAVI 1023
GR SHRHSL+ R++ +IL GH CN +L++HL+RELKG+CQH S I ++
Sbjct: 450 RGRDSHRHSLNGRDNAADILPGHGCNTILEMHLVRELKGNCQH*S*ITIV 301
Score = 115 bits (246), Expect(2) = 1e-033
Identities = 57/112 (50%), Positives = 71/112 (63%)
Frame = -1 / +2
Query: 1028 LQMTAI*DLC*QSPFNSRMRCTFRTALQQWPVNMFVVSFLLCKECRWLYLPPNLV*MLGI 849
++ I D C*Q PFNS RC + LQ WP +M L +ECRWL LP +LV*ML
Sbjct: 296 MRTIVIYDQC*QLPFNSLTRCISKIVLQPWPGSMSAALSLPFRECRWLSLPLDLV*MLNR 475
Query: 848 GCFLASLKLPHLLDGFARVITTI*VWNYLINQMELVKHC*KCSGIIQMLFCA 693
FLASLKL HLL G+A+ +I +YL QM+L *+CSGI++MLFCA
Sbjct: 476 R*FLASLKLQHLLAGYAKATGSIWGSSYLDRQMKLGNLY*ECSGIMKMLFCA 631
Score = 94.1 bits (199), Expect(2) = 4e-026
Identities = 39/76 (51%), Positives = 53/76 (69%)
Frame = -2 / +3
Query: 1006 ICADNRLSIPV*DAPSGQRCSNGPSICS*CHFCCAKSVDGYISLPIWSKCWA*DAFWLP* 827
I ADN LSIP+ DA C++GP++C C ++SVDGY+SL IW +C D FWLP*
Sbjct: 318 ISADNCLSIPLRDASPR*CCNHGPAVCPQHCLCRSESVDGYLSLSIWFEC*TEDNFWLP* 497
Query: 826 SCHTC*MGLPELSLPS 779
SC+TC + +P+L +PS
Sbjct: 498 SCNTCSLDMPKLPVPS 545
Score = 93.6 bits (198), Expect(2) = 1e-024
Identities = 54/105 (51%), Positives = 63/105 (60%)
Frame = +3 / -3
Query: 693 STEQHLDDARAFSTMLHQLHLID*VIPYLDGSDNSGKPI*QVWQLQGSQKASYAQHLDQI 872
ST+ HL D RAFS + QLHL *+ DG+ + G QV QLQGSQK S QH +QI
Sbjct: 631 STK*HLHDPRAFSIKIPQLHLPV*ITRPPDGTGSFGISSEQVLQLQGSQKLSSVQHSNQI 452
Query: 873 GREI*PSTLFAQQK*HYEHIDGPLLQRCPEGASHTGIERRLSAQI 1007
RE *PSTL +Q+ H GP LQ AS GIER+LSA I
Sbjct: 451 ERER*PSTLSERQRQCCGHTAGPWLQHYLGDASRKGIERQLSALI 317
Score = 66.1 bits (138), Expect(2) = 3e-072
Identities = 31/53 (58%), Positives = 34/53 (64%)
Frame = -3 / +1
Query: 1188 IDXXXXDDAPLIXXXXRVIXLDVXXDGVXXGXTLDLASSLDVGSAAPQASGDA 1030
ID DDAPLI RVI LD+ DG G TLDLASSL+ GS QASG+A
Sbjct: 142 IDELFPDDAPLISSGFRVIPLDMKTDGAPTGRTLDLASSLEAGSTTLQASGNA 300
Score = 62.5 bits (130), Expect(2) = 1e-050
Identities = 30/53 (56%), Positives = 32/53 (60%)
Frame = +2 / -2
Query: 1031 ASPEAWGAAEPTSRLDAKSSVXPXXTPSXFTSSXMTRXXXXINGASSXXXXSI 1189
A PEA EP SRL+AKSSV P PS F SS +TR INGASS SI
Sbjct: 299 ALPEACSVVEPASRLEAKSSVLPVGAPSVFMSSGITRKPEDINGASSGKSSSI 141
Score = 49.2 bits (101), Expect(2) = 3e-039
Identities = 25/53 (47%), Positives = 27/53 (50%)
Frame = +3 / -3
Query: 1032 HPLRLGVQQSPHQD*MPNLAXXXXIHHLXSHQVV*HXXXXXSMEHHLXXXHQL 1190
H LRL V + Q * PNL +HHL S VV*H MEHH HQL
Sbjct: 298 HCLRLAV*LNQLQG*RPNLVFYRWVHHLFSCPVV*HGSLKILMEHHQGKAHQL 140
Score = 48.3 bits (99), Expect(2) = 1e-033
Identities = 26/53 (49%), Positives = 27/53 (50%)
Frame = -1 / +2
Query: 1190 QLMXXXQMMLH*XXXXXVSYXLM*XQMVYXXVXR*IWHLVLMWALLHPKPQGM 1032
QLM MMLH* V Y * QMV+ V *IW L L LH KPQ M
Sbjct: 140 QLMSFSLMMLH*YLQASVLYHWT*KQMVHPPVEH*IWPLALKLVQLHCKPQAM 298
Score = 44.6 bits (91), Expect(2) = 4e-026
Identities = 22/46 (47%), Positives = 25/46 (54%)
Frame = -2 / +3
Query: 1168 *CSIDXXXLSCHTT*CEXRWCXXXXXARFGI*S*CGLCCTPSLRGC 1031
*CSI+ L C+TT E RWC RFG+* * T SLR C
Sbjct: 162 *CSINIFRLPCYTTGHENRWCTHR*NTRFGL*P*SWFNYTASLRQC 299
Score = 40.0 bits (81), Expect(2) = 1e-024
Identities = 23/52 (44%), Positives = 26/52 (50%)
Frame = +1 / -1
Query: 1033 IP*GLGCSRAHIKTRCQI*RXTXXYTIXXHIKXYDTXXXXNQWSIIXXXXIN 1188
I *GL CS K R QI* T TI H++ Y+T WSII IN
Sbjct: 297 IA*GLQCS*TSFKARGQI*CSTGGCTICFHVQWYNTEA*RY*WSIIREKLIN 142
>gb|BQ470070.1|BQ470070 HX01K23T HX Hordeum vulgare subsp. vulgare cDNA clone HX01K23
5-PRIME, mRNA sequence
Length = 633
Score = 270 bits (585), Expect = 8e-072
Identities = 103/141 (73%), Positives = 124/141 (87%)
Frame = -3 / +1
Query: 798 QSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFKEKPMFTFANKAGLDMLETSLV 619
QSY +HLG+EL Q+D AGE+LL+MLW H DA+LCCSFKEKP+FTFAN+ G++MLETS V
Sbjct: 10 QSYRFHLGVELFRQADEAGESLLRMLWDHEDAILCCSFKEKPVFTFANEMGINMLETSFV 189
Query: 618 ALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAVAWKVLGE 439
A QDL+LDKIFDE+GRKAL+S+I KLMEQG+ YLP GVC+SGMGRHVSF+ A+AWKV+GE
Sbjct: 190 ATQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGE 369
Query: 438 DSNIHCLAFCFVNWSFV*LKS 376
D+N+HCLAFC VNWSF * S
Sbjct: 370 DNNVHCLAFCLVNWSFE*SSS 432
Score = 207 bits (447), Expect = 8e-053
Identities = 93/133 (69%), Positives = 102/133 (76%)
Frame = +2 / -2
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENS 565
H+KDQLTKQ ARQ LLSSP+TFHA A+SKETWRPIPD HTP GR PCSI+F +SE S
Sbjct: 422 HSKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 243
Query: 566 AFLPDSSKILSSVRSCSATRDVSNMSSPALFANVNIGFSLKEQHRTASG*CQSIFNNASP 745
AFLP SS ILS+ RS AT+DVSNM P FANVN GFSLKEQH+ AS * QSI N SP
Sbjct: 242 AFLPASSNILSNERS*VATKDVSNMLIPISFANVNTGFSLKEQHKIASS*SQSILNKDSP 63
Query: 746 APSD*LSNSIPRW 784
A S L+NS PRW
Sbjct: 62 ASSACLNNSTPRW 24
Score = 116 bits (249), Expect = 2e-025
Identities = 61/138 (44%), Positives = 80/138 (57%)
Frame = +1 / -1
Query: 385 SHEGPVDEAERQAVDVAILAEHLPRYSLVERNMXXXXXXXXXXRQVRVALFH*FRDV*E* 564
S E PVD AE +AV + +L ++ P YS++E +M QV AL H D+ *
Sbjct: 423 SLERPVD*AEGKAVHIVVLTDYFPCYSILEGDMAAHPRQAHTTWQVNKALLHQLWDLRV* 244
Query: 565 CFPS*LVEDLV*RQVLQRNKRCF*HVQPCLVCKRKHRFLLKGAAQNSIWMMPEHFQQCFT 744
C + ++ LV R+VL N+RCF HV P LVCKRK+R L+ AQNSI+M+PEH Q F
Sbjct: 243 CLSTSFIKYLVQREVLSGNERCFQHVNPHLVCKRKYRLFLERTAQNSIFMIPEHSQ*RFP 64
Query: 745 SSI*LIK*FHT*MVVITL 798
S I L K* M + L
Sbjct: 63 SFICLSK*LDPQMEPVAL 10
>gb|BF626725.2|BF626725 HVSMEb0001C22f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001C22f, mRNA sequence
Length = 834
Score = 237 bits (513), Expect = 7e-062
Identities = 94/151 (62%), Positives = 118/151 (78%)
Frame = -3 / -3
Query: 837 GFPEAATLARWVCQSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFKEKPMFTFA 658
G EA TLARW+ +SY +H G ELL + +A LK LW H D+++CCS K P+FTFA
Sbjct: 724 GSLEAHTLARWIGRSYRFHTGAELLCTESQSADASLKALWQHSDSIMCCSLKAAPVFTFA 545
Query: 657 NKAGLDMLETSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHV 478
N+AGLDMLET+L+ALQD++L+KI D+ GRKAL S+ K+M+QG+AYLP GVC+S M R V
Sbjct: 544 NQAGLDMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGGVCVSSMRRPV 365
Query: 477 SFDQAVAWKVLGEDSNIHCLAFCFVNWSFV* 385
S++QAVAWKVLG+D HCLAF FVNWSFV*
Sbjct: 364 SYEQAVAWKVLGDDDAPHCLAFMFVNWSFV* 272
Score = 130 bits (278), Expect = 2e-029
Identities = 64/119 (53%), Positives = 73/119 (61%)
Frame = +2 / +3
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENS 565
+TKDQLT ARQ SSPSTFHATA S +T R + D HTP GRYA PC + SE+S
Sbjct: 273 YTKDQLTNMKARQCGASSSPSTFHATACSYDTGRLMLDTHTPPGRYAKPCCMILGNSEHS 452
Query: 566 AFLPDSSKILSSVRSCSATRDVSNMSSPALFANVNIGFSLKEQHRTASG*CQSIFNNAS 742
A P SS+I SS S SA VS+MS PA A VN G + +EQH S C S F AS
Sbjct: 453 ALRPSSSRIFSSEMSWSAISVVSSMSRPAWLAKVNTGAAFREQHMIESECCHSAFREAS 629
>gb|BM378002.2|BM378002 EBem04_SQ004_N18_R embryo, 12 DPA, no treatment, cv Optic, EBem04
Hordeum vulgare subsp. vulgare cDNA clone
EBem04_SQ004_N18 5', mRNA sequence
Length = 540
Score = 177 bits (381), Expect(2) = 1e-047
Identities = 69/121 (57%), Positives = 90/121 (74%)
Frame = -3 / +3
Query: 798 QSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFKEKPMFTFANKAGLDMLETSLV 619
+SY H G E+ ++ LK+LW+H DA+LCCS K PMFTF N A LDMLET+LV
Sbjct: 3 RSYRAHTGEEIRWSDTEEADSPLKLLWNHSDAILCCSLKPAPMFTFGNNAALDMLETTLV 182
Query: 618 ALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAVAWKVLGE 439
LQD++L+ I D+ GRKAL ++ SK+M+QG+AYLP GVC S MGR S++QAVAWKV+G+
Sbjct: 183 NLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAVAWKVVGD 362
Query: 438 D 436
D
Sbjct: 363 D 365
Score = 107 bits (228), Expect(2) = 3e-024
Identities = 49/91 (53%), Positives = 59/91 (64%)
Frame = +2 / -3
Query: 437 SSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENSAFLPDSSKILSSVRSCS 616
SSP+TFHATA S + RP+ D+HTP GRYA PC ++ E S +SA P SS+I SS SC
Sbjct: 364 SSPTTFHATACSYDACRPMLDLHTPPGRYAKPCCMTLENSAHSALRPSSSRIASSEMSCR 185
Query: 617 ATRDVSNMSSPALFANVNIGFSLKEQHRTAS 709
T VS+MS AL VNIG +EQ AS
Sbjct: 184 FTSVVSSMSRAALLPKVNIGAGFREQQSMAS 92
Score = 33.1 bits (66), Expect(2) = 1e-047
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / +3
Query: 426 HCLAFCFVNWSFV* 385
HCLAF FVNW+F+*
Sbjct: 381 HCLAFMFVNWTFL* 422
Score = 24.9 bits (48), Expect(2) = 3e-024
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +1 / -2
Query: 382 QSHEGPVDEAERQAV 426
+S EGPVDE E +AV
Sbjct: 425 RSQEGPVDEHEGKAV 381
>gb|BE231148.2|BE231148 HVSMEg0017A24f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017A24f, mRNA sequence
Length = 905
Score = 138 bits (295), Expect(2) = 2e-042
Identities = 61/111 (54%), Positives = 80/111 (72%)
Frame = -3 / +3
Query: 1014 NLRSVLTIAFQFPYEMHLQDSVAAMARQYVRSVISAVQRVSMAISPSQSGLNAGHRMLSG 835
N RSVLTIAFQF YE HL++SVAAMARQYVR+V+++VQRV+MAI+PS+ G + +G
Sbjct: 309 NTRSVLTIAFQFSYENHLRESVAAMARQYVRTVVASVQRVAMAIAPSRPGGQIEMKNPAG 488
Query: 834 FPEAATLARWVCQSYHYHLGMELLNQSDGAGEALLKMLWHHPDAVLCCSFK 682
PEA TLARW+ +SY +H G ELL + +A L L H D+++C S K
Sbjct: 489 SPEAHTLARWIGRSYMFHTGAELLCTESQSADAYL*ALGQHSDSIMC*SLK 641
Score = 83.1 bits (175), Expect(2) = 2e-021
Identities = 52/112 (46%), Positives = 60/112 (53%)
Frame = +2 / -2
Query: 680 SLKEQHRTASG*CQSIFNNASPAPSD*LSNSIPRW***LWQTHLASVAASGKPESILCPA 859
+ ++QH S C S + AS S+S P *L HLASV ASG P
Sbjct: 643 AFRDQHMIESECCPSAYR*ASALCDSVQSSSAPV*NM*LLPIHLASV*ASGDPAGFFISI 464
Query: 860 FRPDWEGDIAIDTLCTAEMTLRTY*RAIAATLS*RCISYGN*KAIVSTDLKL 1015
P EG IA+ TLCT T+ TY AIAATLS R SY N KA+VSTDL L
Sbjct: 463 CPPGREGAIAMATLCTDATTVLTYCLAIAATLSRRWFSYENWKAMVSTDLVL 308
Score = 55.1 bits (114), Expect(2) = 2e-042
Identities = 26/54 (48%), Positives = 30/54 (55%)
Frame = -3 / +3
Query: 1188 IDXXXXDDAPLIXXXXRVIXLDVXXDGVXXGXTLDLASSLDVGSAAPQASGDAS 1027
ID DDAPL+ RVI LD D TLDLAS+L+VG +A DAS
Sbjct: 138 IDESFADDAPLLPSGFRVIPLDAKTDAPSATRTLDLASTLEVGPGGTRAPSDAS 299
Score = 40.0 bits (81), Expect(2) = 2e-021
Identities = 24/54 (44%), Positives = 28/54 (51%)
Frame = +2 / -2
Query: 1028 DASPEAWGAAEPTSRLDAKSSVXPXXTPSXFTSSXMTRXXXXINGASSXXXXSI 1189
DAS A PTSR+DA+SSV S SS +TR +GASS SI
Sbjct: 298 DASLGARVPPGPTSRVDARSSVRVADGASVLASSGITRKPEGSSGASSANDSSI 137
>gb|BE438011.1|BE438011 SFR009.H06F990511 ITEC SFR Barley Leaf Epidermis Library Hordeum
vulgare subsp. vulgare cDNA clone SF, mRNA sequence
Length = 523
Score = 170 bits (366), Expect = 1e-041
Identities = 65/82 (79%), Positives = 76/82 (92%)
Frame = -3 / +3
Query: 621 VALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAVAWKVLG 442
VALQDL+LDKIFDE+GRKAL+S+I KLMEQG+ YLP GVC+SGMGRHVSF+ A+AWKV+G
Sbjct: 6 VALQDLSLDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVG 185
Query: 441 EDSNIHCLAFCFVNWSFV*LKS 376
ED+N+HCLAFCFVNWSFV* S
Sbjct: 186 EDNNVHCLAFCFVNWSFV*SSS 251
Score = 133 bits (284), Expect = 3e-030
Identities = 58/80 (72%), Positives = 64/80 (80%)
Frame = +2 / -1
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENS 565
HTKDQLTKQ ARQ LLSSP+TFHA A+SKETWRPIPD HTP GR PCSI+F +SE S
Sbjct: 241 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 62
Query: 566 AFLPDSSKILSSVRSCSATR 625
AFLP SS ILS+ RS AT+
Sbjct: 61 AFLPASSNILSNERS*RATK 2
>gb|BF254204.2|BF254204 HVSMEf0003E24f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEf0003E24f, mRNA sequence
Length = 860
Score = 76.7 bits (161), Expect(5) = 7e-039
Identities = 30/45 (66%), Positives = 41/45 (91%)
Frame = -3 / +3
Query: 1008 RSVLTIAFQFPYEMHLQDSVAAMARQYVRSVISAVQRVSMAISPS 874
++V+TIAFQF +E HLQDSVA MARQY+RS+IS+VQR+++A+S S
Sbjct: 375 KAVMTIAFQFAFESHLQDSVAVMARQYMRSIISSVQRIALALSSS 509
Score = 47.8 bits (98), Expect(5) = 7e-039
Identities = 16/29 (55%), Positives = 22/29 (75%)
Frame = -3 / +3
Query: 831 PEAATLARWVCQSYHYHLGMELLNQSDGA 745
PEAATL+RW+ QSY +H G EL+ +D +
Sbjct: 555 PEAATLSRWIVQSYRFHFGAELIKPADAS 641
Score = 43.2 bits (88), Expect(5) = 7e-039
Identities = 19/51 (37%), Positives = 29/51 (56%)
Frame = -3 / +3
Query: 1188 IDXXXXDDAPLIXXXXRVIXLDVXXDGVXXGXTLDLASSLDVGSAAPQASG 1036
ID DD+PL+ R+I ++ D TLDLAS+L+VG+ + +G
Sbjct: 204 IDASFSDDSPLLPSGFRIIPIESPLDTSSPNCTLDLASTLEVGTPGSRITG 356
Score = 39.6 bits (80), Expect(5) = 7e-039
Identities = 15/30 (50%), Positives = 23/30 (76%)
Frame = -3 / +3
Query: 666 TFANKAGLDMLETSLVALQDLTLDKIFDES 577
TFAN++GLDML T+L A + T+D++ D +
Sbjct: 720 TFANQSGLDMLXTTLAAPPEDTMDRVLDST 809
Score = 27.2 bits (53), Expect(5) = 7e-039
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = -3 / +2
Query: 717 HHPDAVLCCSFK 682
HH A+LCCS K
Sbjct: 671 HHTSAILCCSLK 706
>gb|BJ455677.1|BJ455677 BJ455677 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 3', mRNA sequence
Length = 420
Score = 157 bits (338), Expect = 9e-038
Identities = 59/75 (78%), Positives = 69/75 (92%)
Frame = -3 / -3
Query: 600 LDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAVAWKVLGEDSNIHC 421
LDKIFDE+GRKAL+S+I KLMEQG+ YLP GVC+SGMGRHVSF+ A+AWKV+GED+N+HC
Sbjct: 418 LDKIFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHC 239
Query: 420 LAFCFVNWSFV*LKS 376
LAFCFVNWSFV* S
Sbjct: 238 LAFCFVNWSFV*SSS 194
Score = 127 bits (271), Expect = 2e-028
Identities = 54/72 (75%), Positives = 59/72 (81%)
Frame = +2 / +3
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENS 565
HTKDQLTKQ ARQ LLSSP+TFHA A+SKETWRPIPD HTP GR PCSI+F +SE S
Sbjct: 204 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 383
Query: 566 AFLPDSSKILSS 601
AFLP SS ILS+
Sbjct: 384 AFLPASSNILSN 419
>gb|BJ447934.1|BJ447934 BJ447934 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak17m20 5', mRNA sequence
Length = 447
Score = 150 bits (323), Expect = 1e-035
Identities = 56/72 (77%), Positives = 66/72 (91%)
Frame = -3 / +1
Query: 591 IFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAVAWKVLGEDSNIHCLAF 412
IFDE+GRKAL+S+I KLMEQG+ YLP GVC+SGMGRHVSF+ A+AWKV+GED+N+HCLAF
Sbjct: 13 IFDEAGRKALYSEIPKLMEQGFVYLPGGVCLSGMGRHVSFENAIAWKVVGEDNNVHCLAF 192
Query: 411 CFVNWSFV*LKS 376
CFVNWSFV* S
Sbjct: 193 CFVNWSFV*SSS 228
Score = 122 bits (261), Expect = 4e-027
Identities = 52/70 (74%), Positives = 56/70 (80%)
Frame = +2 / -2
Query: 386 HTKDQLTKQNARQWMLLSSPSTFHATAWSKETWRPIPDMHTPDGRYA*PCSISFEMSENS 565
HTKDQLTKQ ARQ LLSSP+TFHA A+SKETWRPIPD HTP GR PCSI+F +SE S
Sbjct: 218 HTKDQLTKQKARQCTLLSSPTTFHAIAFSKETWRPIPDKHTPPGR*TKPCSINFGISEYS 39
Query: 566 AFLPDSSKIL 595
AFLP SS L
Sbjct: 38 AFLPASSNXL 9
>gb|AJ462332.1|AJ462332 AJ462332 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200065F09F1, mRNA sequence
Length = 360
Score = 111 bits (238), Expect(2) = 5e-028
Identities = 43/68 (63%), Positives = 57/68 (83%)
Frame = -3 / +3
Query: 639 MLETSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAV 460
MLET+LV LQD++L+ I D+ GRKAL ++ SK+M+QG+AYLP GVC S MGR S++QAV
Sbjct: 3 MLETTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAV 182
Query: 459 AWKVLGED 436
AWKV+G+D
Sbjct: 183 AWKVVGDD 206
Score = 33.1 bits (66), Expect(2) = 5e-028
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / +3
Query: 426 HCLAFCFVNWSFV* 385
HCLAF FVNW+F+*
Sbjct: 222 HCLAFMFVNWTFL* 263
>gb|AL499972.1|AL499972 AL499972 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK04F09r 3', mRNA sequence
Length = 573
Score = 85.4 bits (180), Expect(3) = 2e-027
Identities = 31/40 (77%), Positives = 36/40 (90%)
Frame = -3 / -3
Query: 495 GMGRHVSFDQAVAWKVLGEDSNIHCLAFCFVNWSFV*LKS 376
GMGRHVSF+ A+AWKV+GED+N+ CLAFCFVNWSFV* S
Sbjct: 364 GMGRHVSFENAIAWKVVGEDNNVXCLAFCFVNWSFV*SSS 245
Score = 40.5 bits (82), Expect(3) = 2e-027
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = -3 / -2
Query: 555 DISKLMEQGYAYLPSGVCMSGMG 487
+I KLMEQG+ YLP GVC+S G
Sbjct: 425 EIPKLMEQGFVYLPGGVCLSWDG 357
Score = 35.0 bits (70), Expect(3) = 2e-027
Identities = 16/31 (51%), Positives = 21/31 (67%)
Frame = -3 / -2
Query: 648 GLDMLETSLVALQDLTLDKIFDESGRKALFS 556
GL + VALQDL+LDKIFDE+ K ++
Sbjct: 521 GLTCWKHLFVALQDLSLDKIFDEAW*KGTYT 429
>gb|BQ664839.1|BQ664839 HX01B05w HX Hordeum vulgare subsp. vulgare cDNA clone HX01B05
3-PRIME, mRNA sequence
Length = 489
Score = 105 bits (224), Expect(2) = 4e-026
Identities = 40/65 (61%), Positives = 54/65 (83%)
Frame = -3 / -1
Query: 630 TSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQAVAWK 451
T+LV LQD++L+ I D+ GRKAL ++ SK+M+QG+AYLP GVC S MGR S++QAVAWK
Sbjct: 489 TTLVNLQDISLEAILDDEGRKALCAEFSKVMQQGFAYLPGGVCKSSMGRQASYEQAVAWK 310
Query: 450 VLGED 436
V+G+D
Sbjct: 309 VVGDD 295
Score = 33.1 bits (66), Expect(2) = 4e-026
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / -1
Query: 426 HCLAFCFVNWSFV* 385
HCLAF FVNW+F+*
Sbjct: 279 HCLAFMFVNWTFL* 238
>gb|BI957471.1|BI957471 HVSMEn0009M03f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0009M03f, mRNA sequence
Length = 718
Score = 109 bits (233), Expect = 3e-023
Identities = 44/68 (64%), Positives = 57/68 (83%)
Frame = -3 / +1
Query: 642 DMLETSLVALQDLTLDKIFDESGRKALFSDISKLMEQGYAYLPSGVCMSGMGRHVSFDQA 463
DMLET+L+ALQD++L+KI D+ GRKAL S+ K+M+QG+AYLP GVC+S MGR VS++ A
Sbjct: 25 DMLETTLIALQDISLEKILDDDGRKALCSEFPKIMQQGFAYLPGGVCVSSMGRPVSYEXA 204
Query: 462 VAWKVLGE 439
VA KVL E
Sbjct: 205 VAXKVLAE 228
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 594,214,406
Number of Sequences: 312970
Number of extensions: 10101594
Number of successful extensions: 58046
Number of sequences better than 1.0e-020: 23
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 14334233767
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)