TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2448387.2.1
(919 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 ... 116 3e-040
gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedl... 129 3e-039
gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa... 134 4e-038
gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hyd... 107 2e-037
gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedl... 123 7e-037
gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgar... 100 1e-034
gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA li... 104 4e-034
gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. v... 132 2e-033
gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, dro... 88 1e-031
gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA li... 121 2e-031
gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedl... 85 8e-030
gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke devel... 119 9e-028
gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. v... 122 3e-027
gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke devel... 76 8e-026
gb|BI956935.1|BI956935 HVSMEn0006D04f Hordeum vulgare rachi... 68 3e-025
gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots... 86 5e-025
gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachi... 85 4e-023
gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. v... 73 4e-022
gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa... 81 3e-021
>gb|BE454671.3|BE454671 HVSMEh0094P16f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0094P16f, mRNA sequence
Length = 685
Score = 116 bits (249), Expect(2) = 3e-040
Identities = 49/90 (54%), Positives = 67/90 (74%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KE+IKYKGFQVAPAELEA+L++HP ++DAAV L D+ AGEVP + V+R G+ +E D+
Sbjct: 412 KEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDI 591
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGK 463
+VA V YK++ + F D+IPK+ SGK
Sbjct: 592 KKFVAKEVVFYKRIHKVFFTDSIPKNPSGK 681
Score = 68.9 bits (144), Expect(2) = 3e-040
Identities = 25/44 (56%), Positives = 31/44 (70%)
Frame = -2 / +1
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
DTG SL +N PGEIC+R + +M+GY E T+ TID GWLHT
Sbjct: 226 DTGASLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHT 357
>gb|BF064865.2|BF064865 HV_CEb0021D12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0021D12f, mRNA sequence
Length = 806
Score = 129 bits (277), Expect(2) = 3e-039
Identities = 55/96 (57%), Positives = 71/96 (73%)
Frame = -2 / +3
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KELIKYKG+QV PAELEA+LL+HP V D AV PD E G+ P + VVR++G+ S ++
Sbjct: 312 KELIKYKGYQVPPAELEALLLTHPEVSDVAVIPFPDREVGQFPMAYVVRKKGSNLSAQEV 491
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQL 445
M +VA +VA YKK+R + FV IPK+ SGKILR+ L
Sbjct: 492 MEFVAKQVAPYKKVRKVAFVTDIPKNASGKILRKDL 599
Score = 52.4 bits (108), Expect(2) = 3e-039
Identities = 19/44 (43%), Positives = 28/44 (63%)
Frame = -2 / +3
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
+TG +LP N GE+ +R VM+GY++ E T+ T+ GWL T
Sbjct: 126 ETGEALPVNRTGELWIRGPYVMKGYFKNTEATQSTVTPDGWLKT 257
>gb|BG414540.1|BG414540 HVSMEk0002N03f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0002N03f, mRNA sequence
Length = 876
Score = 134 bits (288), Expect(2) = 4e-038
Identities = 55/96 (57%), Positives = 73/96 (76%)
Frame = -2 / +3
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KELIKYKGFQ+APAELE +LLSHP + DA V PD+EAGEVP + VVR + +E D+
Sbjct: 366 KELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDV 545
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQL 445
++A +V YK+LR + FV+++PKS +GKILRR+L
Sbjct: 546 QKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRREL 653
Score = 43.7 bits (89), Expect(2) = 4e-038
Identities = 17/36 (47%), Positives = 23/36 (63%)
Frame = -2 / +3
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTI 811
+T + LP + GEIC+R +MQGY+ E TE TI
Sbjct: 183 ETQKHLPPSQLGEICIRGPHIMQGYFNNVEATEFTI 290
>gb|BQ763608.1|BQ763608 EBro02_SQ008_M23_R root, 3 week, hydroponic grown, low nitrogen, cv
Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone
EBro02_SQ008_M23 5', mRNA sequence
Length = 590
Score = 107 bits (228), Expect(2) = 2e-037
Identities = 45/85 (52%), Positives = 62/85 (72%)
Frame = -2 / +3
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KE+IKYKGFQVAPAELEA+L++HP ++DAAV L D+ AGEVP + V+R G+ +E D+
Sbjct: 336 KEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDI 515
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPK 478
+VA YK++ + F D+IPK
Sbjct: 516 KKFVAKEGVFYKRIHKVFFTDSIPK 590
Score = 68.9 bits (144), Expect(2) = 2e-037
Identities = 25/44 (56%), Positives = 31/44 (70%)
Frame = -2 / +3
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
DTG SL +N PGEIC+R + +M+GY E T+ TID GWLHT
Sbjct: 150 DTGASLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHT 281
>gb|BE213865.1|BE213865 HV_CEb0001H21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0001H21f, mRNA sequence
Length = 922
Score = 123 bits (263), Expect(2) = 7e-037
Identities = 51/97 (52%), Positives = 73/97 (75%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KE++KYKGFQVAPAELEA+L++HP ++DAAV L D+ AGEVP + V+R G+ +E D+
Sbjct: 172 KEIMKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDI 351
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQLR 442
+VA V YK++ + F D++PK+ SG+ILR+ LR
Sbjct: 352 KKFVAKEVGFYKRIHKVFFTDSMPKNPSGEILRKDLR 462
Score = 51.0 bits (105), Expect(2) = 7e-037
Identities = 20/39 (51%), Positives = 25/39 (64%)
Frame = -2 / +1
Query: 903 LPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
L +N PGE VR + +M+GY E T+ TID GWLHT
Sbjct: 1 LGRNQPGERGVRGEHIMKGYLNDPESTKNTIDKDGWLHT 117
>gb|CK124865.1|CK124865 BES1824110k15 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010K1510 5-PRIME, mRNA sequence
Length = 630
Score = 100 bits (212), Expect(3) = 1e-034
Identities = 44/85 (51%), Positives = 59/85 (69%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KELIK+KGFQV PAELEA+L++HPS+ DAAV D+ AGEVP + VVR + +E +
Sbjct: 307 KELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADSDIAEEAI 486
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPK 478
+V+ +V YK+L + F AIPK
Sbjct: 487 KEFVSKQVVFYKRLHKVYFTHAIPK 561
Score = 61.6 bits (128), Expect(3) = 1e-034
Identities = 24/44 (54%), Positives = 28/44 (63%)
Frame = -2 / +1
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
DTG SL +N PGEIC+R +M+GY T TID GWLHT
Sbjct: 121 DTGLSLARNLPGEICIRGPQIMKGYLDDPVATAATIDVEGWLHT 252
Score = 23.1 bits (44), Expect(3) = 1e-034
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = -2 / +2
Query: 483 PKSVSGKILRRQLRDE 436
P S SGKILR++ + +
Sbjct: 557 PSSASGKILRKRAQSQ 604
>gb|AV916926.1|AV916926 AV916926 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 5', mRNA sequence
Length = 547
Score = 104 bits (222), Expect(3) = 4e-034
Identities = 43/75 (57%), Positives = 55/75 (73%)
Frame = -2 / +2
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KELIKYKGFQ+APAELE +LLSHP + DA V PD+EAGEVP + VVR + +E D+
Sbjct: 248 KELIKYKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDV 427
Query: 552 MAYVAGRVASYKKLR 508
++A +V YK+LR
Sbjct: 428 QKFIAKQVTYYKRLR 472
Score = 43.7 bits (89), Expect(3) = 4e-034
Identities = 17/36 (47%), Positives = 23/36 (63%)
Frame = -2 / +2
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTI 811
+T + LP + GEIC+R +MQGY+ E TE TI
Sbjct: 65 ETQKHLPPSQLGEICIRGPHIMQGYFNNVEATEFTI 172
Score = 35.0 bits (70), Expect(3) = 4e-034
Identities = 13/20 (65%), Positives = 18/20 (90%)
Frame = -2 / +3
Query: 504 LRFVDAIPKSVSGKILRRQL 445
L FV+++PKS +GKILRR+L
Sbjct: 477 LPFVESVPKSAAGKILRREL 536
>gb|BU986484.1|BU986484 HF12A02r HF Hordeum vulgare subsp. vulgare cDNA clone HF12A02
5-PRIME, mRNA sequence
Length = 577
Score = 132 bits (282), Expect(2) = 2e-033
Identities = 57/96 (59%), Positives = 72/96 (75%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KELIKYKG+QV PAELE IL SHP + DAAV G PDE+ G++P + +VR+ G+ + +
Sbjct: 145 KELIKYKGYQVPPAELEHILQSHPEIADAAVIGYPDEDVGQLPMAFIVRQPGSSLTGKQV 324
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQL 445
M YVA VA YKK+R + FV AIPKS +GKILRR+L
Sbjct: 325 MDYVAKHVAPYKKVRRVAFVAAIPKSPAGKILRREL 432
Score = 30.4 bits (60), Expect(2) = 2e-033
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -2 / +1
Query: 858 VMQGYYRRKEETERTIDAAGWLHT 787
VM+GY + T T+D+ GWL T
Sbjct: 19 VMKGYVGDDKATAETVDSEGWLKT 90
>gb|BQ766207.1|BQ766207 EBro08_SQ005_H07_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ005_H07 5', mRNA sequence
Length = 552
Score = 87.7 bits (185), Expect(2) = 1e-031
Identities = 36/60 (60%), Positives = 48/60 (80%)
Frame = -2 / +3
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KE+IKYKGFQVAPAELEA+L++HP ++DAAV L D+ AGEVP + V+R G+ +E D+
Sbjct: 366 KEIIKYKGFQVAPAELEALLITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDI 545
Score = 68.9 bits (144), Expect(2) = 1e-031
Identities = 25/44 (56%), Positives = 31/44 (70%)
Frame = -2 / +3
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
DTG SL +N PGEIC+R + +M+GY E T+ TID GWLHT
Sbjct: 180 DTGASLGRNQPGEICIRGEQIMKGYLNDPESTKNTIDKDGWLHT 311
>gb|AV918175.1|AV918175 AV918175 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b24 3', mRNA sequence
Length = 664
Score = 121 bits (259), Expect(2) = 2e-031
Identities = 50/96 (52%), Positives = 69/96 (71%)
Frame = -2 / -3
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
K + KGFQ+APAELE +LLSHP + DA V PD+EAGEVP + VVR + +E D+
Sbjct: 518 KS*LSTKGFQIAPAELEGLLLSHPEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDV 339
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQL 445
++A +V YK+LR + FV+++PKS +GKILRR+L
Sbjct: 338 QKFIAKQVTYYKRLRRVTFVESVPKSAAGKILRREL 231
Score = 34.5 bits (69), Expect(2) = 2e-031
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = -2 / -1
Query: 879 ICVRSQAVMQGYYRRKEETERTIDA 805
IC+R +MQGY+ E TE TI A
Sbjct: 664 ICIRGPHIMQGYFNNVEATEFTIQA 590
>gb|BF623201.2|BF623201 HVSMEa0015K22f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0015K22f, mRNA sequence
Length = 817
Score = 85.4 bits (180), Expect(2) = 8e-030
Identities = 36/72 (50%), Positives = 50/72 (69%)
Frame = -2 / +3
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KE+IKYKGFQV PAELE +L++H ++DAAV + DE GEVP + +VR G+ SE+++
Sbjct: 543 KEIIKYKGFQVPPAELEPLLITHAEIKDAAVVSMQDELTGEVPVAFIVRIEGSEISESEI 722
Query: 552 MAYVAGRVASYK 517
+ A V S K
Sbjct: 723 KQFGAKEVVSTK 758
Score = 65.2 bits (136), Expect(2) = 8e-030
Identities = 24/44 (54%), Positives = 30/44 (68%)
Frame = -2 / +3
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
DTG SL +N PGEIC+R + +M+GY E T+ T D GWLHT
Sbjct: 357 DTGASLARNLPGEICIRGKQIMKGYLNDPEATKNTTDKDGWLHT 488
>gb|AL507037.1|AL507037 AL507037 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21T 5',
mRNA sequence
Length = 700
Score = 119 bits (254), Expect(2) = 9e-028
Identities = 53/97 (54%), Positives = 69/97 (71%)
Frame = -2 / -2
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KELIK+KGFQV PAELEA L++HPS+ DAAV D+ AGEVP + VVR + +E +
Sbjct: 603 KELIKFKGFQVPPAELEAXLIAHPSIADAAVVPQKDDAAGEVPVAFVVRAADSDIAEEAI 424
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQLR 442
+V+ +V YK+L + F AIPKS SGKILR++LR
Sbjct: 423 KEFVSKQVVFYKRLHKVYFTHAIPKSASGKILRKELR 313
Score = 24.4 bits (47), Expect(2) = 9e-028
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -2 / -3
Query: 819 RTIDAAGWLHT 787
R ID GWLHT
Sbjct: 692 RPIDVEGWLHT 660
>gb|CB882386.1|CB882386 HL01L15w HL Hordeum vulgare subsp. vulgare cDNA clone HL01L15
3-PRIME, mRNA sequence
Length = 490
Score = 122 bits (261), Expect = 3e-027
Identities = 50/97 (51%), Positives = 71/97 (73%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADM 553
KE+IKYKGFQV PAELEA+L++H ++DAAV + DE GEVP + +VR G+ SE+++
Sbjct: 79 KEIIKYKGFQVPPAELEALLITHAEIKDAAVVSMQDELTGEVPVAFIVRIEGSEISESEI 258
Query: 552 MAYVAGRVASYKKLRLLRFVDAIPKSVSGKILRRQLR 442
+VA V YK++ + F D++PKS +GKILR+ LR
Sbjct: 259 KQFVAKEVVFYKRIHKVFFADSVPKSPAGKILRKDLR 369
>gb|AL510457.1|AL510457 AL510457 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY04P21u 3',
mRNA sequence
Length = 508
Score = 75.8 bits (159), Expect(2) = 8e-026
Identities = 32/50 (64%), Positives = 39/50 (78%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRR 583
KELIK+KGFQV PAELEA+L++HPS+ DAAV D+ AGEVP + V R
Sbjct: 358 KELIKFKGFQVPPAELEALLIAHPSIADAAVVPQKDDAAGEVPVAFVGAR 507
Score = 61.6 bits (128), Expect(2) = 8e-026
Identities = 24/44 (54%), Positives = 28/44 (63%)
Frame = -2 / +1
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
DTG SL +N PGEIC+R +M+GY T TID GWLHT
Sbjct: 172 DTGLSLARNLPGEICIRGPQIMKGYLDDPVATAATIDVEGWLHT 303
>gb|BI956935.1|BI956935 HVSMEn0006D04f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0006D04f, mRNA sequence
Length = 654
Score = 68.4 bits (143), Expect(2) = 3e-025
Identities = 27/43 (62%), Positives = 35/43 (81%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVP 604
KE+IKYKGFQVAPA+LEA+L++HP ++D V L D+ A EVP
Sbjct: 505 KEIIKYKGFQVAPAKLEALLITHPEIQDXGVVFLKDDLACEVP 633
Score = 67.0 bits (140), Expect(2) = 3e-025
Identities = 24/44 (54%), Positives = 31/44 (70%)
Frame = -2 / +1
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
DTG SL +N PGEIC+R + +M+GY + T+ TID GWLHT
Sbjct: 319 DTGASLGRNQPGEICIRGEQIMKGYLNDPKSTKNTIDKDGWLHT 450
>gb|AL502418.1|AL502418 AL502418 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07I12u 3', mRNA sequence
Length = 592
Score = 86.3 bits (182), Expect(2) = 5e-025
Identities = 35/73 (47%), Positives = 51/73 (69%)
Frame = -2 / -1
Query: 663 PSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADMMAYVAGRVASYKKLRLLRFVDAI 484
P + DA V PD+EAGEVP + VVR + +E D+ ++A +V YK+LR + FV+++
Sbjct: 520 PEILDAVVIPFPDDEAGEVPIAYVVRSPASSLTEVDVQKFIAKQVTYYKRLRRVTFVESV 341
Query: 483 PKSVSGKILRRQL 445
PKS +GKILRR+L
Sbjct: 340 PKSAAGKILRREL 302
Score = 48.3 bits (99), Expect(2) = 5e-025
Identities = 19/24 (79%), Positives = 21/24 (87%)
Frame = -2 / -3
Query: 732 KELIKYKGFQVAPAELEAILLSHP 661
KELIK KGFQ+APAE E +LLSHP
Sbjct: 590 KELIKSKGFQIAPAEXEGLLLSHP 519
>gb|BI959839.1|BI959839 HVSMEn0021P10f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0021P10f, mRNA sequence
Length = 657
Score = 85.4 bits (180), Expect(2) = 4e-023
Identities = 36/77 (46%), Positives = 53/77 (68%)
Frame = -2 / -1
Query: 672 LSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGAPESEADMMAYVAGRVASYKKLRLLRFV 493
++HP ++DAAV L D+ AGEVP + V+R G+ +E D+ +VA V YK++ + F
Sbjct: 552 ITHPEIKDAAVVSLKDDLAGEVPIAFVMRIEGSEITEDDIKKFVAKEVVFYKRIHKVFFT 373
Query: 492 DAIPKSVSGKILRRQLR 442
D+IPK+ SGKILR+ LR
Sbjct: 372 DSIPKNPSGKILRKDLR 322
Score = 42.8 bits (87), Expect(2) = 4e-023
Identities = 17/18 (94%), Positives = 18/18 (100%)
Frame = -2 / -3
Query: 732 KELIKYKGFQVAPAELEA 679
KE+IKYKGFQVAPAELEA
Sbjct: 613 KEIIKYKGFQVAPAELEA 560
>gb|CA007491.1|CA007491 HU08C03r HU Hordeum vulgare subsp. vulgare cDNA clone HU08C03
5-PRIME, mRNA sequence
Length = 581
Score = 72.5 bits (152), Expect(2) = 4e-022
Identities = 28/43 (65%), Positives = 33/43 (76%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVP 604
KELIKYKG+Q+ PAELEA+LL+HP V D AV PD E G+ P
Sbjct: 442 KELIKYKGYQLPPAELEALLLTHPEVSDVAVIPFPDREVGQFP 570
Score = 52.4 bits (108), Expect(2) = 4e-022
Identities = 19/44 (43%), Positives = 28/44 (63%)
Frame = -2 / +1
Query: 918 DTGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
+TG +LP N GE+ +R VM+GY++ E T+ T+ GWL T
Sbjct: 256 ETGEALPVNRTGELWIRGPYVMKGYFKNTEATQSTVTPDGWLKT 387
>gb|BG418054.1|BG418054 HVSMEk0021B11f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0021B11f, mRNA sequence
Length = 491
Score = 80.8 bits (170), Expect(2) = 3e-021
Identities = 32/53 (60%), Positives = 41/53 (77%)
Frame = -2 / +1
Query: 732 KELIKYKGFQVAPAELEAILLSHPSVEDAAVFGLPDEEAGEVPASCVVRRRGA 574
KELIKYKG+Q PAELE IL SHP + DAAV G PDE+ G++P + +VR+ G+
Sbjct: 316 KELIKYKGYQGPPAELEHILQSHPEIADAAVIGYPDEDVGQLPMAFIVRQPGS 474
Score = 40.9 bits (83), Expect(2) = 3e-021
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = -2 / +1
Query: 915 TGRSLPKNPPGEICVRSQAVMQGYYRRKEETERTIDAAGWLHT 787
TG +L GE+ ++ VM+GY + T T+D+ GWL T
Sbjct: 133 TGEALWPGQRGELLIKGPLVMKGYVGDDKATAETVDSEGWLKT 261
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 399,830,247
Number of Sequences: 312970
Number of extensions: 5679522
Number of successful extensions: 64777
Number of sequences better than 1.0e-020: 25
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 10652245967
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)