TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440806.2.2
(1155 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU995294.1|BU995294 HM09M17r HM Hordeum vulgare subsp. v... 228 1e-096
gb|BU981349.1|BU981349 HA23F03r HA Hordeum vulgare subsp. v... 314 4e-085
gb|BI960587.1|BI960587 HVSMEn0025E05f Hordeum vulgare rachi... 271 4e-072
gb|CB881433.1|CB881433 HM09M17w HM Hordeum vulgare subsp. v... 222 3e-057
gb|BM442096.2|BM442096 EBan01_SQ002_C23_R anther, yellow st... 209 2e-053
gb|AV915392.1|AV915392 AV915392 K. Sato unpublished cDNA li... 171 9e-042
gb|CA001634.1|CA001634 HS05C08r HS Hordeum vulgare subsp. v... 165 4e-040
gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachi... 91 1e-032
>gb|BU995294.1|BU995294 HM09M17r HM Hordeum vulgare subsp. vulgare cDNA clone HM09M17
5-PRIME, mRNA sequence
Length = 585
Score = 228 bits (492), Expect(2) = 1e-096
Identities = 86/96 (89%), Positives = 92/96 (95%)
Frame = +2 / +1
Query: 428 QDAVMQVTVAFNHFGRGLVQRMPRCRYGFFHVVNNDYTHWIMYAIGGNMNPTIISQGNRF 607
QD +MQVTVAFNHFG+GLVQRMPRCR+GFFHVVNNDYTHW MYAIGGN +PTIISQGNRF
Sbjct: 298 QDRMMQVTVAFNHFGKGLVQRMPRCRFGFFHVVNNDYTHWKMYAIGGNRDPTIISQGNRF 477
Query: 608 IAPDDPNAKEVTKREYTPYKDYKEWVWKSQGDVMMN 715
IAPDDPNAKEVTKREYTPY +YK+WVWKSQGDVMMN
Sbjct: 478 IAPDDPNAKEVTKREYTPYNEYKDWVWKSQGDVMMN 585
Score = 193 bits (417), Expect(2) = 3e-089
Identities = 80/96 (83%), Positives = 82/96 (85%)
Frame = -1 / -2
Query: 714 FIMTSPCDFHTHSL*SLYGVYSRLVTSLALGSSGAMKRLPWLMMVGFMLPPMAYMIQCV* 535
FIMTSPCDFHT SL SL GVYSRLVTSLALGSSGAM R PWLMMVG LPPMAY+ QCV*
Sbjct: 584 FIMTSPCDFHTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSRLPPMAYIFQCV* 405
Query: 534 SLFTTWKKP*RQRGIRCTSPRPKWLNATVTCITASC 427
SLFTTWKKP RQRG+RCT P PKWL ATVTCI SC
Sbjct: 404 SLFTTWKKPKRQRGMRCTRPLPKWLKATVTCIILSC 297
Score = 155 bits (334), Expect(2) = 3e-089
Identities = 62/89 (69%), Positives = 71/89 (79%)
Frame = -1 / -2
Query: 441 ITASCGLSLLAPNSITWSWSVKWLLDTVMAVDPFTTSISPSEQLDMDTWSIHTLLDDSTE 262
I + C +LLAP S+TWSW VKWLL+TVMAV+P TTS+ PS Q+DMDTWSIHTLL++ E
Sbjct: 308 ILSCCSWALLAPKSMTWSWLVKWLLETVMAVEPLTTSMRPSAQVDMDTWSIHTLLEERME 129
Query: 261 TPSPSLRVRSP*CRFESRIMPPECALESW 175
TPSPSLRVR P* ESRIMPPEC E W
Sbjct: 128 TPSPSLRVRRP*WCLESRIMPPECGAEWW 42
Score = 146 bits (313), Expect(2) = 1e-096
Identities = 60/83 (72%), Positives = 64/83 (77%)
Frame = +2 / +1
Query: 182 SKAHSGGMIRDSKRHYGLRTRXXXXXXXXXXXXNVWIDHVSMSSCSDGLIDVVNGSTAIT 361
S HSGGMIRDSK HYG RTR NVWIDHVSMS+C+DGLIDVV+GSTAIT
Sbjct: 49 SAPHSGGMIRDSKHHYGRRTRSDGDGVSILSSSNVWIDHVSMSTCADGLIDVVSGSTAIT 228
Query: 362 VSNSHFTDHDHVMLFGASNDSPQ 430
VSNSHFT+HDHVMLFGASN Q
Sbjct: 229 VSNSHFTNHDHVMLFGASNAQEQ 297
Score = 87.2 bits (184), Expect(2) = 7e-027
Identities = 35/57 (61%), Positives = 44/57 (77%)
Frame = +1 / +3
Query: 271 VVQQRVDRPRVHVQLLRRADRRGERVDGHHRVQQPLHRPRPRDAVRRQQRQPAGRGD 441
+++QR+DRPRVHV L RR RR +R+ HHR+QQPLH+PRPR A+RRQQR A D
Sbjct: 138 LLKQRMDRPRVHVHLRRRPHRRRQRLHRHHRLQQPLHQPRPRHALRRQQRPGAAGQD 308
Score = 56.5 bits (117), Expect(2) = 7e-027
Identities = 22/27 (81%), Positives = 25/27 (92%)
Frame = +1 / +3
Query: 634 GGDQAGVHAVQGLQGVGVEVAGRRHDE 714
GGDQAGVHA+Q +QG+GVEVAG RHDE
Sbjct: 504 GGDQAGVHALQRVQGLGVEVAGGRHDE 584
>gb|BU981349.1|BU981349 HA23F03r HA Hordeum vulgare subsp. vulgare cDNA clone HA23F03
5-PRIME, mRNA sequence
Length = 509
Score = 314 bits (681), Expect = 4e-085
Identities = 121/144 (84%), Positives = 132/144 (91%)
Frame = +2 / +3
Query: 446 VTVAFNHFGRGLVQRMPRCRYGFFHVVNNDYTHWIMYAIGGNMNPTIISQGNRFIAPDDP 625
VTVAFNHFG+GLVQRMPRCR+GFFHVVNNDYTHW MYAIGGN +PTIISQGNRFIAPDDP
Sbjct: 12 VTVAFNHFGKGLVQRMPRCRFGFFHVVNNDYTHWKMYAIGGNRDPTIISQGNRFIAPDDP 191
Query: 626 NAKEVTKREYTPYKDYKEWVWKSQGDVMMNGAFFNESGGQNERKYDRFDFIPAKHGRYVG 805
NAKEVTKREYTPY +YK+WVWKSQGDVMMNGAFFN+SGGQNER Y DFIPAKHG+YVG
Sbjct: 192 NAKEVTKREYTPYNEYKDWVWKSQGDVMMNGAFFNQSGGQNERTYGNMDFIPAKHGKYVG 371
Query: 806 QLTRFAGPLKCIVGQPC**TAARS 877
QLT+FAG L+C VG+PC** + S
Sbjct: 372 QLTQFAGTLECRVGKPC**NSIAS 443
Score = 262 bits (567), Expect = 2e-069
Identities = 112/144 (77%), Positives = 116/144 (80%)
Frame = -1 / -2
Query: 876 DRAAVY*HGWPTMHLSGPAKRVS*PT*RPCLAGMKSNLSYLRSFWPPDSLKKAPFIMTSP 697
D +*HG PT H S PA VS PT PCLAGMKS L Y+RSFWPPD LKKAPFIMTSP
Sbjct: 442 DAIEFH*HGLPTRHSSVPANCVSCPTYLPCLAGMKSMLPYVRSFWPPDWLKKAPFIMTSP 263
Query: 696 CDFHTHSL*SLYGVYSRLVTSLALGSSGAMKRLPWLMMVGFMLPPMAYMIQCV*SLFTTW 517
CDFHT SL SL GVYSRLVTSLALGSSGAM R PWLMMVG LPPMAY+ QCV*SLFTTW
Sbjct: 262 CDFHTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSRLPPMAYIFQCV*SLFTTW 83
Query: 516 KKP*RQRGIRCTSPRPKWLNATVT 445
KKP RQRG+RCT P PKWL ATVT
Sbjct: 82 KKPKRQRGMRCTRPLPKWLKATVT 11
Score = 121 bits (258), Expect = 1e-026
Identities = 54/76 (71%), Positives = 62/76 (81%)
Frame = +1 / +2
Query: 634 GGDQAGVHAVQGLQGVGVEVAGRRHDEWRLLQRVRRPERAQVRQVRLHPGQARPLRRSAH 813
GGDQAGVHA+Q +QG+GVEVAG RHDE LLQ VRRPERA VRQ LHP QAR +RR+AH
Sbjct: 200 GGDQAGVHALQRVQGLGVEVAGGRHDERGLLQPVRRPERAHVRQHGLHPCQARQVRRAAH 379
Query: 814 ALRRTTQVHRRPAVLV 861
A+RR ++ RR AVLV
Sbjct: 380 AVRRHARMPRRQAVLV 427
>gb|BI960587.1|BI960587 HVSMEn0025E05f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025E05f, mRNA sequence
Length = 663
Score = 271 bits (587), Expect = 4e-072
Identities = 108/191 (56%), Positives = 135/191 (70%)
Frame = +2 / +1
Query: 281 NVWIDHVSMSSCSDGLIDVVNGSTAITVSNSHFTDHDHVMLFGASNDSPQDAVMQVTVAF 460
+VW+DH S+S+C+DGLID + GSTAITVSN++FT H+ VML G S+ +D MQVT+AF
Sbjct: 40 HVWVDHCSLSNCADGLIDAIMGSTAITVSNNYFTHHNEVMLLGHSDSYLKDKAMQVTIAF 219
Query: 461 NHFGRGLVQRMPRCRYGFFHVVNNDYTHWIMYAIGGNMNPTIISQGNRFIAPDDPNAKEV 640
NHFG GL+QRMPRCR+G+FHVVNNDYTHW MYAIGG+ PTI SQGNR++AP +P AKEV
Sbjct: 220 NHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEV 399
Query: 641 TKREYTPYKDYKEWVWKSQGDVMMNGAFFNESGGQNERKYDRFDFIPAKHGRYVGQLTRF 820
TKR T +K W W+S+GD+++NGAFF S Y R + K V +T
Sbjct: 400 TKRVETAQTTWKAWNWRSEGDMLLNGAFFTPSRAXASAIYSRAXSLGGKSPSMVATITSG 579
Query: 821 AGPLKCIVGQP 853
AG L C P
Sbjct: 580 AGALSCHKAPP 612
Score = 169 bits (363), Expect = 3e-041
Identities = 82/153 (53%), Positives = 93/153 (60%)
Frame = -1 / -2
Query: 735 DSLKKAPFIMTSPCDFHTHSL*SLYGVYSRLVTSLALGSSGAMKRLPWLMMVGFMLPPMA 556
D +KKAPF SP D + ++ V + VTSLA G GA R PWL+MVG LPPMA
Sbjct: 494 DGVKKAPFSSMSPSDLQFQAFHVVWAVSTLFVTSLAKGLVGAR*RFPWLLMVGSALPPMA 315
Query: 555 YMIQCV*SLFTTWKKP*RQRGIRCTSPRPKWLNATVTCITASCGLSLLAPNSITWSWSVK 376
Y+ Q V*SLFTTWK P GI SP PKWL A VTCI S L P SIT W VK
Sbjct: 314 YISQ*V*SLFTTWK*PCLHLGILWMSPSPKWLKAMVTCIALSLR*ESL*PRSITSLWWVK 135
Query: 375 WLLDTVMAVDPFTTSISPSEQLDMDTWSIHTLL 277
LLDTVMAV+P S+ PS QL+ + WS T L
Sbjct: 134 *LLDTVMAVEPMMASMRPSAQLEREQWSTQTWL 36
>gb|CB881433.1|CB881433 HM09M17w HM Hordeum vulgare subsp. vulgare cDNA clone HM09M17
3-PRIME, mRNA sequence
Length = 490
Score = 222 bits (479), Expect = 3e-057
Identities = 87/107 (81%), Positives = 96/107 (89%)
Frame = +2 / -1
Query: 557 AIGGNMNPTIISQGNRFIAPDDPNAKEVTKREYTPYKDYKEWVWKSQGDVMMNGAFFNES 736
AIGGN +PTIISQGNRFIAPDDPNAKEVTKREYTPY +YK+WVWKSQGDVMMNGAFFN+S
Sbjct: 490 AIGGNPDPTIISQGNRFIAPDDPNAKEVTKREYTPYNEYKDWVWKSQGDVMMNGAFFNQS 311
Query: 737 GGQNERKYDRFDFIPAKHGRYVGQLTRFAGPLKCIVGQPC**TAARS 877
GGQNER Y DFIPAKHG+YVGQLT+FAG L+C VG+PC** + S
Sbjct: 310 GGQNERTYGNMDFIPAKHGKYVGQLTQFAGTLECRVGKPC**NSIAS 170
Score = 184 bits (396), Expect = 9e-046
Identities = 81/106 (76%), Positives = 83/106 (78%)
Frame = -1 / +3
Query: 876 DRAAVY*HGWPTMHLSGPAKRVS*PT*RPCLAGMKSNLSYLRSFWPPDSLKKAPFIMTSP 697
D +*HG PT H S PA VS PT PCLAGMKS L Y+RSFWPPD LKKAPFIMTSP
Sbjct: 171 DAIEFH*HGLPTRHSSVPANCVSCPTYLPCLAGMKSMLPYVRSFWPPDWLKKAPFIMTSP 350
Query: 696 CDFHTHSL*SLYGVYSRLVTSLALGSSGAMKRLPWLMMVGFMLPPM 559
CDFHT SL SL GVYSRLVTSLALGSSGAM R PWLMMVG LPPM
Sbjct: 351 CDFHTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSGLPPM 488
Score = 121 bits (258), Expect = 1e-026
Identities = 54/76 (71%), Positives = 62/76 (81%)
Frame = +1 / -3
Query: 634 GGDQAGVHAVQGLQGVGVEVAGRRHDEWRLLQRVRRPERAQVRQVRLHPGQARPLRRSAH 813
GGDQAGVHA+Q +QG+GVEVAG RHDE LLQ VRRPERA VRQ LHP QAR +RR+AH
Sbjct: 413 GGDQAGVHALQRVQGLGVEVAGGRHDERGLLQPVRRPERAHVRQHGLHPCQARQVRRAAH 234
Query: 814 ALRRTTQVHRRPAVLV 861
A+RR ++ RR AVLV
Sbjct: 233 AVRRHARMPRRQAVLV 186
>gb|BM442096.2|BM442096 EBan01_SQ002_C23_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_C23 5', mRNA sequence
Length = 356
Score = 209 bits (451), Expect = 2e-053
Identities = 80/117 (68%), Positives = 97/117 (82%)
Frame = +2 / +2
Query: 422 SPQDAVMQVTVAFNHFGRGLVQRMPRCRYGFFHVVNNDYTHWIMYAIGGNMNPTIISQGN 601
+P+D +MQVTVAFNHFGRGLVQRMPRCR+GFFHVVNNDYTHW+MYAIGG+ NPTIISQGN
Sbjct: 2 NPKDQIMQVTVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSKNPTIISQGN 181
Query: 602 RFIAPDDPNAKEVTKREYTPYKDYKEWVWKSQGDVMMNGAFFNESGGQNERKYDRFD 772
R+IAP + AK +TKR ++K WVW SQ D+++N A F +SGG NERK+D+ D
Sbjct: 182 RYIAPPNLAAKLITKRLGASEAEWKNWVWHSQDDLLLNDAVFADSGGANERKFDKDD 352
Score = 147 bits (316), Expect = 1e-034
Identities = 69/118 (58%), Positives = 74/118 (62%)
Frame = -1 / -3
Query: 774 KSNLSYLRSFWPPDSLKKAPFIMTSPCDFHTHSL*SLYGVYSRLVTSLALGSSGAMKRLP 595
+S+LS S PP S K A F S C+ HT S SRLV S A GAM R P
Sbjct: 354 RSSLSNFLSLAPPLSAKTASFSSRSSCECHTQFFHSASDAPSRLVISFAARFGGAM*RFP 175
Query: 594 WLMMVGFMLPPMAYMIQCV*SLFTTWKKP*RQRGIRCTSPRPKWLNATVTCITASCGL 421
WLMMVGF+LPPMAYM Q V*SLFTTWK P R GI CT P PKWL ATVTC+ S GL
Sbjct: 174 WLMMVGFLLPPMAYMSQWV*SLFTTWKNPHRHLGILCTRPLPKWLKATVTCMIWSLGL 1
>gb|AV915392.1|AV915392 AV915392 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags14d11 5', mRNA sequence
Length = 554
Score = 171 bits (367), Expect = 9e-042
Identities = 69/127 (54%), Positives = 85/127 (66%)
Frame = +2 / +3
Query: 491 MPRCRYGFFHVVNNDYTHWIMYAIGGNMNPTIISQGNRFIAPDDPNAKEVTKREYTPYKD 670
MPRCR+G+FHVVNNDYTHW MYAIGG+ PTI SQGNR++AP +P AKEVTKR T
Sbjct: 21 MPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEVTKRVETAQTT 200
Query: 671 YKEWVWKSQGDVMMNGAFFNESGGQNERKYDRFDFIPAKHGRYVGQLTRFAGPLKCIVGQ 850
+K W W+S+GD+++NGAFF SG Y R + AK V +T AG L C G
Sbjct: 201 WKAWNWRSEGDMLLNGAFFTPSGAGASASYSRASSLGAKSSSMVATITSGAGALSCHKGS 380
Query: 851 PC**TAA 871
C* +A+
Sbjct: 381 SC*HSAS 401
>gb|CA001634.1|CA001634 HS05C08r HS Hordeum vulgare subsp. vulgare cDNA clone HS05C08
5-PRIME, mRNA sequence
Length = 521
Score = 165 bits (355), Expect = 4e-040
Identities = 67/125 (53%), Positives = 83/125 (66%)
Frame = +2 / +1
Query: 497 RCRYGFFHVVNNDYTHWIMYAIGGNMNPTIISQGNRFIAPDDPNAKEVTKREYTPYKDYK 676
RCR+G+FHVVNNDYTHW MYAIGG+ PTI SQGNR++AP +P AKEVTKR T +K
Sbjct: 13 RCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEVTKRVETAQTTWK 192
Query: 677 EWVWKSQGDVMMNGAFFNESGGQNERKYDRFDFIPAKHGRYVGQLTRFAGPLKCIVGQPC 856
W W+S+GD+++NGAFF SG Y R + AK V +T AG L C G C
Sbjct: 193 AWNWRSEGDMLLNGAFFTPSGAGASASYSRASSLGAKSSSMVATITSGAGALSCHKGSSC 372
Query: 857 **TAA 871
* +A+
Sbjct: 373 *HSAS 387
>gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018A17f, mRNA sequence
Length = 632
Score = 91.3 bits (193), Expect(2) = 1e-032
Identities = 37/77 (48%), Positives = 48/77 (62%)
Frame = +2 / +3
Query: 179 DSKAHSGGMIRDSKRHYGLRTRXXXXXXXXXXXXNVWIDHVSMSSCSDGLIDVVNGSTAI 358
D + M+R S HYG RT +VW+DH S+S+C+DGLID + GSTAI
Sbjct: 384 DCRPTGNAMVRSSPSHYGWRTIADGDAVXIFGASHVWVDHCSLSNCADGLIDAIMGSTAI 563
Query: 359 TVSNSHFTDHDHVMLFG 409
TVSN++FT H+ ML G
Sbjct: 564 TVSNNYFTHHNEGMLLG 614
Score = 78.0 bits (164), Expect(2) = 1e-022
Identities = 39/79 (49%), Positives = 44/79 (55%)
Frame = -1 / -2
Query: 408 PNSITWSWSVKWLLDTVMAVDPFTTSISPSEQLDMDTWSIHTLLDDSTETPSPSLRVRSP 229
P SI W VK LLDTVMAV+P S+SPS QL+ + WS T L T SPS VR P
Sbjct: 613 PRSIPSLWWVK*LLDTVMAVEPMMASMSPSAQLEREQWSTQTWLAPKMXTASPSAMVRHP 434
Query: 228 *CRFESRIMPPECALESWM 172
* E R M +SWM
Sbjct: 433 *WLGELRTMALPVGRQSWM 377
Score = 68.9 bits (144), Expect(2) = 1e-032
Identities = 28/37 (75%), Positives = 30/37 (81%)
Frame = +2 / +3
Query: 5 DRPLWIVFARDMVIELRQELIVNHNKTIDGRGAQVHI 115
D PLWI+F RDMVI L QELI+N KTIDGRGA VHI
Sbjct: 207 DEPLWIIFKRDMVITLSQELIMNSFKTIDGRGANVHI 317
Score = 49.2 bits (101), Expect(2) = 1e-022
Identities = 22/37 (59%), Positives = 25/37 (67%)
Frame = -3 / -1
Query: 115 DVHLRAPPVDRLVVVHDQLLPQLDHHVAREHDPQRPV 5
DVH+ A VD L VHD+LL + D HVA E DPQR V
Sbjct: 317 DVHVGAAAVDGLEAVHDELLAEGDDHVALEDDPQRLV 207
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 396,173,513
Number of Sequences: 312970
Number of extensions: 6287917
Number of successful extensions: 75176
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 13832744539
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)